ALPK1 cDNA ORF clone, Bos taurus(cattle)

The following ALPK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235495 XM_005207668.4
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235495 XM_010805924.3
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235495 XM_005207667.4
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235495 XM_010805923.3
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235495 XM_024993503.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235495 XM_010805922.3
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235496 XM_015471513.2
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235497 XM_024993504.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235498 XM_024993505.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa235499 XM_024993506.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa90061 XM_015471515.2
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa90061 XM_015463701.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa20201 XM_010805924.2
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20201 XM_005207667.3
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20201 XM_010805922.2
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20201 XM_005207668.3
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20201 XM_010805923.2
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20201 XM_015463695.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20201 XM_015463696.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20201 XM_015463697.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20201 XM_015463693.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20201 XM_015463694.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20202 XM_015463699.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20202 XM_015471513.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20203 XM_015463700.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20203 XM_015471514.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20205 XM_015471516.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20205 XM_015463702.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa50880 XM_015471512.2
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa50880 XM_015463698.1
Latest version!
Bos taurus alpha kinase 1 (ALPK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa90061 XM_015471515.1 Bos taurus alpha kinase 1 (ALPK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa50880 XM_015471512.1 Bos taurus alpha kinase 1 (ALPK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235495
    Clone ID Related Accession (Same CDS sequence) XM_005207667.4 , XM_024993503.1 , XM_010805922.3 , XM_010805924.3 , XM_005207668.4 , XM_010805923.3
    Accession Version XM_005207667.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207667.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207724.1
    CDS238..3966
    Translation

    Target ORF information:

    RefSeq Version XM_005207667.4
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207667.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235495
    Clone ID Related Accession (Same CDS sequence) XM_005207667.4 , XM_024993503.1 , XM_010805922.3 , XM_010805924.3 , XM_005207668.4 , XM_010805923.3
    Accession Version XM_024993503.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849271.1
    CDS426..4154
    Translation

    Target ORF information:

    RefSeq Version XM_024993503.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235495
    Clone ID Related Accession (Same CDS sequence) XM_005207667.4 , XM_024993503.1 , XM_010805922.3 , XM_010805924.3 , XM_005207668.4 , XM_010805923.3
    Accession Version XM_010805922.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805922.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804224.1
    CDS290..4018
    Translation

    Target ORF information:

    RefSeq Version XM_010805922.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805922.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235495
    Clone ID Related Accession (Same CDS sequence) XM_005207667.4 , XM_024993503.1 , XM_010805922.3 , XM_010805924.3 , XM_005207668.4 , XM_010805923.3
    Accession Version XM_010805924.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804226.1
    CDS374..4102
    Translation

    Target ORF information:

    RefSeq Version XM_010805924.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805924.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235495
    Clone ID Related Accession (Same CDS sequence) XM_005207667.4 , XM_024993503.1 , XM_010805922.3 , XM_010805924.3 , XM_005207668.4 , XM_010805923.3
    Accession Version XM_005207668.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207668.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207725.1
    CDS120..3848
    Translation

    Target ORF information:

    RefSeq Version XM_005207668.4
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207668.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235495
    Clone ID Related Accession (Same CDS sequence) XM_005207667.4 , XM_024993503.1 , XM_010805922.3 , XM_010805924.3 , XM_005207668.4 , XM_010805923.3
    Accession Version XM_010805923.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804225.1
    CDS171..3899
    Translation

    Target ORF information:

    RefSeq Version XM_010805923.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805923.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235496
    Clone ID Related Accession (Same CDS sequence) XM_015471513.2
    Accession Version XM_015471513.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGGATG
    TGGTATGAAG CAGCTGAGTT AATATGGGCC TCAATCATGG GATACCTGAC ACTTCCTCAG
    CCAGACAAAA AGGGCATTTC CACATCACTA GGTATATTGG CAGACATCTT CGTCTCCATG
    AGCAAGAAAG ATTATGAAAA GTTTAAAAGC AATCCTGACA TTAACCTGGG CTTGCTGAAG
    GAGTTTGACC ATCACTTGCT GTCAGCCGCT GAGGCCTGCA AGCTGGCGGC TGCCTTCAGC
    CCCTACACGC CCCTGTTCGT GCTCACGGCT ATGAATATCC GTGGCACGTG CCTCTTGTCA
    TATAGTAGTT CTGAGGACTG TCCTCCAGGA ATGAAAAATT CATATTTATA TGAAGCCAAA
    GAAGCCTTTG AAATTGGCCT CCTCACCAAG AAACGTGATG AACCAGTTAC TGGAAGACAG
    GAGCTCCATA GTTTTCTTAA AGCTGCTTTT GGTCTCACCA CTGTGCACCA AAGACTCTAT
    GGGGAGATGG AGACCGTCCG AACAGCAAGT CAGCTCTGTA GTGAAGCTAT GGGAAAGCTG
    TATACTTTCA GCAATTCCTC CAGAAGTCAG GAGAGAGAAG CTGTCTCTCA GGAAATCATG
    TCTGTTATCA CCCGGGTGAA GGAACAGTTA CAAGTTCAAA GCTTCTCAAA TTTAGATGAC
    AAGTCTTACG TTCCAGAGGG ATTCAAGCGT GGGTTAGAGA AGCCCATCTT ACATGGGCAA
    GTGGATTTCC CAAAAATCCT TGAAGCCTAT TCACAGCACC ACACTTCAGT TTGTGAACTA
    TTTGAAAGCA CTTGTGGGAA CAACAAAAAT AAACAGAAAG GTATGAAAAC AGGAGTCTGT
    ATCACAGATC TAAAGACAGA AACACAAAAC ACAGATACCG TGAGTGCCAC GGAGGACAAA
    GCACATTTTG AAAAAGGCAG GGTGATATCT TCTTCCCGAA TGGCGATGAA CAGTCAGGAG
    AAACTCAGGA GGGCGGGAAG GAGAAACTGG GCCGGTTCTG ATGCGTTTCG AGTCTCCGTG
    GATGAAGATG TGGAGACCGA AGCTGAGTCA ACAGACCACA GCAATGGTGG GGGAGCTGTC
    CTGAACAAGT CTCTGAGTGA CAGCCTGAGC TCCAGTTCTT GGAGCCAGTT ATCAGGGTAC
    AAATCTTCTA CAAGCTGGGA GGAAGTGAAT TATCATGTTG ATGATATGTC TGCCGGAAGA
    GAGCTGAGCA AGGAGGGGCA TCTTGTGGAC ACGCAGTGTT CCACTGCTCT GTCAGACGAG
    CAGGAGGTGA ATGGGGTGAG CAGAGCTGAG CACCCACTGT CTTCAGAGCT GCATGGTCTC
    TCTCTCCAGG TGCCCAGGGA TGACAGGTTA GAGTCTTCTC AATGTCAGCT GCACAAGTCC
    ACGCCCTTGG CAACCTTCCC ACCTCACAGC ACAACAGGCA CTTCATTGGC CTCTGGTGGA
    GGGTGGTTTA AAGAAGGCAT GCAGGAAGTT GAAAATGTGG GGTCCAGAAA TACTTCTGCT
    CATTCCAGAC CCTTATTTTG TTCTGCTTCT TGGACTTCTG ATTCTGGTTG GCCTAAGAAC
    ATGACCACGT ATCCTTCCAT CCAAGAAGAA GAAACCTTTG AAACAATTGG TGAGTTTCAA
    GAAATCAACG GAGATGCCAA GGATGGGGAT GAAGAAGAGA AGAGAGAAGA AATCAAAGGA
    GGCACAGGTC CTCCATTTAA AGATAGCCCC TGCTGGGTTG ACCCAGAAGG AAAAATAGCA
    GAAAGAGCAG AAGATGTTCC CGTAGGCTTC CACTCAGTCG TGGACGAGTC TCGGGACAAA
    AGCTGCATGG TAGCATGTAA CTCCTACACT CCTCATTGGC TTGTTGAAAA CCCTAACTCG
    GGAAAAAATG GTCTCTCAGT CAAAGAGCAG GGCATTGACC CTGATGCCTC CACGGTGGAT
    GAGGAGAGCC CACTGCTGGG CAGCGCAGAT GTTCACACCA CGAGCACACG TGGCTCCCCG
    AGACCGTGCA CTTGGAAGCA GCTGCACGTT CAGGGAGCCC AGATCCCCAG TTCCTCAGTA
    AGCAGAAAGA CTTCCACCCC TGTCCTCAGC GAGGACTGCA CTACCACGGA GGAAGCAAAT
    GAACCTGGAA ACCTGCTAAA CTGCAGCCAG AACTCCAGCT TATCCTCATC TTGGTGGCTG
    AAATCACGGG CAATCTCCAG TGGTTCTTCT GAGGGAGAAA ATCCATGGTC TTTTCTGAAT
    TCCAGTGGAA GTTCTTTTGT TTCATTGCCA GGAATGACAA AGCAGGCGAT CCTTGAGGCC
    CGCACCCTGC AGCCTGATGA CTTTGAAAAA CTCTTGGCAG GGGTGAGGCA TGATTGGTTG
    TTCCAGAGAC TGGAGCATAC AGGCCTTTTT AAATCCAGAC AACTCCACGG AGCACATAGT
    GCTCTTTTGT TAAAATATTC CAAGAAATCT GAACTGTGGA CAGCCCAAGA AACTGTTGTA
    TATTTGGGGG ACTACCTACA TGTGACAAAG AAAGGCAGAC AAAGAAATGC ATTTTGGGTA
    CATCATCTTC ATCAAGAAGA AACTCTGGGC AGATATGTTG GGAAAGAATA TAAGGAGCAA
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGAGGCAGA TGACCGCACA GCACTACGTG
    ACAGAATTTA ACAAGAGACT TTACGAGCAG AAGATTCACA CGCAGATATT CTACATACCG
    TCCTCAGTAT TGCTGATTTT AGAAGGCAAG ACAATAAAAG GATGTGTCAG TGTGGAACCT
    TACATGCTAG GGGAATTTGT AAAATTATCA AATAACACAA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATACGGCTT GGCGTATGGC CACTTTTCTT ATGAATTTTC CAATCATAGA
    GATGTTGTGG TTGATTTACA AGGTTGGGTG ACTGGGAATG GAAAAGGGCT CATCTACCTC
    ACAGATCCCC AGATTCACTC TGTTGATCAG AAAGATGTCA CTACCAATTT TGGAAAGAAA
    GGAATTTTCT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATATG CCATCGTCTT
    TCTTTGACAA GACCTTCGAT TGAGAAACCA AACAATTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326999.1
    CDS290..3931
    Translation

    Target ORF information:

    RefSeq Version XM_015471513.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471513.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGGATG
    TGGTATGAAG CAGCTGAGTT AATATGGGCC TCAATCATGG GATACCTGAC ACTTCCTCAG
    CCAGACAAAA AGGGCATTTC CACATCACTA GGTATATTGG CAGACATCTT CGTCTCCATG
    AGCAAGAAAG ATTATGAAAA GTTTAAAAGC AATCCTGACA TTAACCTGGG CTTGCTGAAG
    GAGTTTGACC ATCACTTGCT GTCAGCCGCT GAGGCCTGCA AGCTGGCGGC TGCCTTCAGC
    CCCTACACGC CCCTGTTCGT GCTCACGGCT ATGAATATCC GTGGCACGTG CCTCTTGTCA
    TATAGTAGTT CTGAGGACTG TCCTCCAGGA ATGAAAAATT CATATTTATA TGAAGCCAAA
    GAAGCCTTTG AAATTGGCCT CCTCACCAAG AAACGTGATG AACCAGTTAC TGGAAGACAG
    GAGCTCCATA GTTTTCTTAA AGCTGCTTTT GGTCTCACCA CTGTGCACCA AAGACTCTAT
    GGGGAGATGG AGACCGTCCG AACAGCAAGT CAGCTCTGTA GTGAAGCTAT GGGAAAGCTG
    TATACTTTCA GCAATTCCTC CAGAAGTCAG GAGAGAGAAG CTGTCTCTCA GGAAATCATG
    TCTGTTATCA CCCGGGTGAA GGAACAGTTA CAAGTTCAAA GCTTCTCAAA TTTAGATGAC
    AAGTCTTACG TTCCAGAGGG ATTCAAGCGT GGGTTAGAGA AGCCCATCTT ACATGGGCAA
    GTGGATTTCC CAAAAATCCT TGAAGCCTAT TCACAGCACC ACACTTCAGT TTGTGAACTA
    TTTGAAAGCA CTTGTGGGAA CAACAAAAAT AAACAGAAAG GTATGAAAAC AGGAGTCTGT
    ATCACAGATC TAAAGACAGA AACACAAAAC ACAGATACCG TGAGTGCCAC GGAGGACAAA
    GCACATTTTG AAAAAGGCAG GGTGATATCT TCTTCCCGAA TGGCGATGAA CAGTCAGGAG
    AAACTCAGGA GGGCGGGAAG GAGAAACTGG GCCGGTTCTG ATGCGTTTCG AGTCTCCGTG
    GATGAAGATG TGGAGACCGA AGCTGAGTCA ACAGACCACA GCAATGGTGG GGGAGCTGTC
    CTGAACAAGT CTCTGAGTGA CAGCCTGAGC TCCAGTTCTT GGAGCCAGTT ATCAGGGTAC
    AAATCTTCTA CAAGCTGGGA GGAAGTGAAT TATCATGTTG ATGATATGTC TGCCGGAAGA
    GAGCTGAGCA AGGAGGGGCA TCTTGTGGAC ACGCAGTGTT CCACTGCTCT GTCAGACGAG
    CAGGAGGTGA ATGGGGTGAG CAGAGCTGAG CACCCACTGT CTTCAGAGCT GCATGGTCTC
    TCTCTCCAGG TGCCCAGGGA TGACAGGTTA GAGTCTTCTC AATGTCAGCT GCACAAGTCC
    ACGCCCTTGG CAACCTTCCC ACCTCACAGC ACAACAGGCA CTTCATTGGC CTCTGGTGGA
    GGGTGGTTTA AAGAAGGCAT GCAGGAAGTT GAAAATGTGG GGTCCAGAAA TACTTCTGCT
    CATTCCAGAC CCTTATTTTG TTCTGCTTCT TGGACTTCTG ATTCTGGTTG GCCTAAGAAC
    ATGACCACGT ATCCTTCCAT CCAAGAAGAA GAAACCTTTG AAACAATTGG TGAGTTTCAA
    GAAATCAACG GAGATGCCAA GGATGGGGAT GAAGAAGAGA AGAGAGAAGA AATCAAAGGA
    GGCACAGGTC CTCCATTTAA AGATAGCCCC TGCTGGGTTG ACCCAGAAGG AAAAATAGCA
    GAAAGAGCAG AAGATGTTCC CGTAGGCTTC CACTCAGTCG TGGACGAGTC TCGGGACAAA
    AGCTGCATGG TAGCATGTAA CTCCTACACT CCTCATTGGC TTGTTGAAAA CCCTAACTCG
    GGAAAAAATG GTCTCTCAGT CAAAGAGCAG GGCATTGACC CTGATGCCTC CACGGTGGAT
    GAGGAGAGCC CACTGCTGGG CAGCGCAGAT GTTCACACCA CGAGCACACG TGGCTCCCCG
    AGACCGTGCA CTTGGAAGCA GCTGCACGTT CAGGGAGCCC AGATCCCCAG TTCCTCAGTA
    AGCAGAAAGA CTTCCACCCC TGTCCTCAGC GAGGACTGCA CTACCACGGA GGAAGCAAAT
    GAACCTGGAA ACCTGCTAAA CTGCAGCCAG AACTCCAGCT TATCCTCATC TTGGTGGCTG
    AAATCACGGG CAATCTCCAG TGGTTCTTCT GAGGGAGAAA ATCCATGGTC TTTTCTGAAT
    TCCAGTGGAA GTTCTTTTGT TTCATTGCCA GGAATGACAA AGCAGGCGAT CCTTGAGGCC
    CGCACCCTGC AGCCTGATGA CTTTGAAAAA CTCTTGGCAG GGGTGAGGCA TGATTGGTTG
    TTCCAGAGAC TGGAGCATAC AGGCCTTTTT AAATCCAGAC AACTCCACGG AGCACATAGT
    GCTCTTTTGT TAAAATATTC CAAGAAATCT GAACTGTGGA CAGCCCAAGA AACTGTTGTA
    TATTTGGGGG ACTACCTACA TGTGACAAAG AAAGGCAGAC AAAGAAATGC ATTTTGGGTA
    CATCATCTTC ATCAAGAAGA AACTCTGGGC AGATATGTTG GGAAAGAATA TAAGGAGCAA
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGAGGCAGA TGACCGCACA GCACTACGTG
    ACAGAATTTA ACAAGAGACT TTACGAGCAG AAGATTCACA CGCAGATATT CTACATACCG
    TCCTCAGTAT TGCTGATTTT AGAAGGCAAG ACAATAAAAG GATGTGTCAG TGTGGAACCT
    TACATGCTAG GGGAATTTGT AAAATTATCA AATAACACAA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATACGGCTT GGCGTATGGC CACTTTTCTT ATGAATTTTC CAATCATAGA
    GATGTTGTGG TTGATTTACA AGGTTGGGTG ACTGGGAATG GAAAAGGGCT CATCTACCTC
    ACAGATCCCC AGATTCACTC TGTTGATCAG AAAGATGTCA CTACCAATTT TGGAAAGAAA
    GGAATTTTCT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATATG CCATCGTCTT
    TCTTTGACAA GACCTTCGAT TGAGAAACCA AACAATTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235497
    Clone ID Related Accession (Same CDS sequence) XM_024993504.1
    Accession Version XM_024993504.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG GCTTCCCTTG
    TGGCTCAGCT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849272.1
    CDS290..3844
    Translation

    Target ORF information:

    RefSeq Version XM_024993504.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG GCTTCCCTTG
    TGGCTCAGCT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235498
    Clone ID Related Accession (Same CDS sequence) XM_024993505.1
    Accession Version XM_024993505.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCAGA GGAGGACAAG AGGGAGGACC AGAGATGCAG AGGCGTCCCT GAAAGCCTCC 
    ATCCTCGCCC GGGACTGCGC CACGGCCGCC GCCATCGTGT TCCTGAGCGA CCGTCTCCTG
    TACGGGCTCG ACGTCTCGGG CCAGCTGCTG CAGGTCGCCA AAGCGCTGCA CAGGCTGCAG
    CCCGCGACGC CCATCGCCCC GCAGGTGGTC ATCCGCCAGG CCCGAATCTC GATGCATGCA
    GGGAAACTTT TAAAAGCAGA GTATATCCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACA
    GGTACCTGGC TATACAGAAA TGAAAGCGAC AAGGTCCTGG TGCAGTCAGT CTGCATACAG
    ATAAGAGGGC AAATTCTGCA GAAGTTGGGG ATGTGGTATG AAGCAGCTGA GTTAATATGG
    GCCTCAATCA TGGGATACCT GACACTTCCT CAGCCAGACA AAAAGGGCAT TTCCACATCA
    CTAGGTATAT TGGCAGACAT CTTCGTCTCC ATGAGCAAGA AAGATTATGA AAAGTTTAAA
    AGCAATCCTG ACATTAACCT GGGCTTGCTG AAGGAGTTTG ACCATCACTT GCTGTCAGCC
    GCTGAGGCCT GCAAGCTGGC GGCTGCCTTC AGCCCCTACA CGCCCCTGTT CGTGCTCACG
    GCTATGAATA TCCGTGGCAC GTGCCTCTTG TCATATAGTA GTTCTGAGGA CTGTCCTCCA
    GGAATGAAAA ATTCATATTT ATATGAAGCC AAAGAAGCCT TTGAAATTGG CCTCCTCACC
    AAGAAACGTG ATGAACCAGT TACTGGAAGA CAGGAGCTCC ATAGTTTTCT TAAAGCTGCT
    TTTGGTCTCA CCACTGTGCA CCAAAGACTC TATGGGGAGA TGGAGACCGT CCGAACAGCA
    AGTCAGCTCT GTAGTGAAGC TATGGGAAAG CTGTATACTT TCAGCAATTC CTCCAGAAGT
    CAGGAGAGAG AAGCTGTCTC TCAGGAAATC ATGTCTGTTA TCACCCGGGT GAAGGAACAG
    TTACAAGTTC AAAGCTTCTC AAATTTAGAT GACAAGTCTT ACGTTCCAGA GGGATTCAAG
    CGTGGGTTAG AGAAGCCCAT CTTACATGGG CAAGTGGATT TCCCAAAAAT CCTTGAAGCC
    TATTCACAGC ACCACACTTC AGTTTGTGAA CTATTTGAAA GCACTTGTGG GAACAACAAA
    AATAAACAGA AAGGTATGAA AACAGGAGTC TGTATCACAG ATCTAAAGAC AGAAACACAA
    AACACAGATA CCGTGAGTGC CACGGAGGAC AAAGCACATT TTGAAAAAGG CAGGGTGATA
    TCTTCTTCCC GAATGGCGAT GAACAGTCAG GAGAAACTCA GGAGGGCGGG AAGGAGAAAC
    TGGGCCGGTT CTGATGCGTT TCGAGTCTCC GTGGATGAAG ATGTGGAGAC CGAAGCTGAG
    TCAACAGACC ACAGCAATGG TGGGGGAGCT GTCCTGAACA AGTCTCTGAG TGACAGCCTG
    AGCTCCAGTT CTTGGAGCCA GTTATCAGGG TACAAATCTT CTACAAGCTG GGAGGAAGTG
    AATTATCATG TTGATGATAT GTCTGCCGGA AGAGAGCTGA GCAAGGAGGG GCATCTTGTG
    GACACGCAGT GTTCCACTGC TCTGTCAGAC GAGCAGGAGG TGAATGGGGT GAGCAGAGCT
    GAGCACCCAC TGTCTTCAGA GCTGCATGGT CTCTCTCTCC AGGTGCCCAG GGATGACAGG
    TTAGAGTCTT CTCAATGTCA GCTGCACAAG TCCACGCCCT TGGCAACCTT CCCACCTCAC
    AGCACAACAG GCACTTCATT GGCCTCTGGT GGAGGGTGGT TTAAAGAAGG CATGCAGGAA
    GTTGAAAATG TGGGGTCCAG AAATACTTCT GCTCATTCCA GACCCTTATT TTGTTCTGCT
    TCTTGGACTT CTGATTCTGG TTGGCCTAAG AACATGACCA CGTATCCTTC CATCCAAGAA
    GAAGAAACCT TTGAAACAAT TGGTGAGTTT CAAGAAATCA ACGGAGATGC CAAGGATGGG
    GATGAAGAAG AGAAGAGAGA AGAAATCAAA GGAGGCACAG GTCCTCCATT TAAAGATAGC
    CCCTGCTGGG TTGACCCAGA AGGAAAAATA GCAGAAAGAG CAGAAGATGT TCCCGTAGGC
    TTCCACTCAG TCGTGGACGA GTCTCGGGAC AAAAGCTGCA TGGTAGCATG TAACTCCTAC
    ACTCCTCATT GGCTTGTTGA AAACCCTAAC TCGGGAAAAA ATGGTCTCTC AGTCAAAGAG
    CAGGGCATTG ACCCTGATGC CTCCACGGTG GATGAGGAGA GCCCACTGCT GGGCAGCGCA
    GATGTTCACA CCACGAGCAC ACGTGGCTCC CCGAGACCGT GCACTTGGAA GCAGCTGCAC
    GTTCAGGGAG CCCAGATCCC CAGTTCCTCA GTAAGCAGAA AGACTTCCAC CCCTGTCCTC
    AGCGAGGACT GCACTACCAC GGAGGAAGCA AATGAACCTG GAAACCTGCT AAACTGCAGC
    CAGAACTCCA GCTTATCCTC ATCTTGGTGG CTGAAATCAC GGGCAATCTC CAGTGGTTCT
    TCTGAGGGAG AAAATCCATG GTCTTTTCTG AATTCCAGTG GAAGTTCTTT TGTTTCATTG
    CCAGGAATGA CAAAGCAGGC GATCCTTGAG GCCCGCACCC TGCAGCCTGA TGACTTTGAA
    AAACTCTTGG CAGGGGTGAG GCATGATTGG TTGTTCCAGA GACTGGAGCA TACAGGCCTT
    TTTAAATCCA GACAACTCCA CGGAGCACAT AGTGCTCTTT TGTTAAAATA TTCCAAGAAA
    TCTGAACTGT GGACAGCCCA AGAAACTGTT GTATATTTGG GGGACTACCT ACATGTGACA
    AAGAAAGGCA GACAAAGAAA TGCATTTTGG GTACATCATC TTCATCAAGA AGAAACTCTG
    GGCAGATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC TCTGGCACCA CTTCACTGAT
    GTGGAGAGGC AGATGACCGC ACAGCACTAC GTGACAGAAT TTAACAAGAG ACTTTACGAG
    CAGAAGATTC ACACGCAGAT ATTCTACATA CCGTCCTCAG TATTGCTGAT TTTAGAAGGC
    AAGACAATAA AAGGATGTGT CAGTGTGGAA CCTTACATGC TAGGGGAATT TGTAAAATTA
    TCAAATAACA CAAAAGTGGT GAAAACAGAA TACAAAGCCA CAGAATACGG CTTGGCGTAT
    GGCCACTTTT CTTATGAATT TTCCAATCAT AGAGATGTTG TGGTTGATTT ACAAGGTTGG
    GTGACTGGGA ATGGAAAAGG GCTCATCTAC CTCACAGATC CCCAGATTCA CTCTGTTGAT
    CAGAAAGATG TCACTACCAA TTTTGGAAAG AAAGGAATTT TCTACTTCTT TAATAACCAG
    CATGTGGAAT GTAATGAAAT ATGCCATCGT CTTTCTTTGA CAAGACCTTC GATTGAGAAA
    CCAAACAATT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849273.1
    CDS505..3999
    Translation

    Target ORF information:

    RefSeq Version XM_024993505.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993505.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCAGA GGAGGACAAG AGGGAGGACC AGAGATGCAG AGGCGTCCCT GAAAGCCTCC 
    ATCCTCGCCC GGGACTGCGC CACGGCCGCC GCCATCGTGT TCCTGAGCGA CCGTCTCCTG
    TACGGGCTCG ACGTCTCGGG CCAGCTGCTG CAGGTCGCCA AAGCGCTGCA CAGGCTGCAG
    CCCGCGACGC CCATCGCCCC GCAGGTGGTC ATCCGCCAGG CCCGAATCTC GATGCATGCA
    GGGAAACTTT TAAAAGCAGA GTATATCCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACA
    GGTACCTGGC TATACAGAAA TGAAAGCGAC AAGGTCCTGG TGCAGTCAGT CTGCATACAG
    ATAAGAGGGC AAATTCTGCA GAAGTTGGGG ATGTGGTATG AAGCAGCTGA GTTAATATGG
    GCCTCAATCA TGGGATACCT GACACTTCCT CAGCCAGACA AAAAGGGCAT TTCCACATCA
    CTAGGTATAT TGGCAGACAT CTTCGTCTCC ATGAGCAAGA AAGATTATGA AAAGTTTAAA
    AGCAATCCTG ACATTAACCT GGGCTTGCTG AAGGAGTTTG ACCATCACTT GCTGTCAGCC
    GCTGAGGCCT GCAAGCTGGC GGCTGCCTTC AGCCCCTACA CGCCCCTGTT CGTGCTCACG
    GCTATGAATA TCCGTGGCAC GTGCCTCTTG TCATATAGTA GTTCTGAGGA CTGTCCTCCA
    GGAATGAAAA ATTCATATTT ATATGAAGCC AAAGAAGCCT TTGAAATTGG CCTCCTCACC
    AAGAAACGTG ATGAACCAGT TACTGGAAGA CAGGAGCTCC ATAGTTTTCT TAAAGCTGCT
    TTTGGTCTCA CCACTGTGCA CCAAAGACTC TATGGGGAGA TGGAGACCGT CCGAACAGCA
    AGTCAGCTCT GTAGTGAAGC TATGGGAAAG CTGTATACTT TCAGCAATTC CTCCAGAAGT
    CAGGAGAGAG AAGCTGTCTC TCAGGAAATC ATGTCTGTTA TCACCCGGGT GAAGGAACAG
    TTACAAGTTC AAAGCTTCTC AAATTTAGAT GACAAGTCTT ACGTTCCAGA GGGATTCAAG
    CGTGGGTTAG AGAAGCCCAT CTTACATGGG CAAGTGGATT TCCCAAAAAT CCTTGAAGCC
    TATTCACAGC ACCACACTTC AGTTTGTGAA CTATTTGAAA GCACTTGTGG GAACAACAAA
    AATAAACAGA AAGGTATGAA AACAGGAGTC TGTATCACAG ATCTAAAGAC AGAAACACAA
    AACACAGATA CCGTGAGTGC CACGGAGGAC AAAGCACATT TTGAAAAAGG CAGGGTGATA
    TCTTCTTCCC GAATGGCGAT GAACAGTCAG GAGAAACTCA GGAGGGCGGG AAGGAGAAAC
    TGGGCCGGTT CTGATGCGTT TCGAGTCTCC GTGGATGAAG ATGTGGAGAC CGAAGCTGAG
    TCAACAGACC ACAGCAATGG TGGGGGAGCT GTCCTGAACA AGTCTCTGAG TGACAGCCTG
    AGCTCCAGTT CTTGGAGCCA GTTATCAGGG TACAAATCTT CTACAAGCTG GGAGGAAGTG
    AATTATCATG TTGATGATAT GTCTGCCGGA AGAGAGCTGA GCAAGGAGGG GCATCTTGTG
    GACACGCAGT GTTCCACTGC TCTGTCAGAC GAGCAGGAGG TGAATGGGGT GAGCAGAGCT
    GAGCACCCAC TGTCTTCAGA GCTGCATGGT CTCTCTCTCC AGGTGCCCAG GGATGACAGG
    TTAGAGTCTT CTCAATGTCA GCTGCACAAG TCCACGCCCT TGGCAACCTT CCCACCTCAC
    AGCACAACAG GCACTTCATT GGCCTCTGGT GGAGGGTGGT TTAAAGAAGG CATGCAGGAA
    GTTGAAAATG TGGGGTCCAG AAATACTTCT GCTCATTCCA GACCCTTATT TTGTTCTGCT
    TCTTGGACTT CTGATTCTGG TTGGCCTAAG AACATGACCA CGTATCCTTC CATCCAAGAA
    GAAGAAACCT TTGAAACAAT TGGTGAGTTT CAAGAAATCA ACGGAGATGC CAAGGATGGG
    GATGAAGAAG AGAAGAGAGA AGAAATCAAA GGAGGCACAG GTCCTCCATT TAAAGATAGC
    CCCTGCTGGG TTGACCCAGA AGGAAAAATA GCAGAAAGAG CAGAAGATGT TCCCGTAGGC
    TTCCACTCAG TCGTGGACGA GTCTCGGGAC AAAAGCTGCA TGGTAGCATG TAACTCCTAC
    ACTCCTCATT GGCTTGTTGA AAACCCTAAC TCGGGAAAAA ATGGTCTCTC AGTCAAAGAG
    CAGGGCATTG ACCCTGATGC CTCCACGGTG GATGAGGAGA GCCCACTGCT GGGCAGCGCA
    GATGTTCACA CCACGAGCAC ACGTGGCTCC CCGAGACCGT GCACTTGGAA GCAGCTGCAC
    GTTCAGGGAG CCCAGATCCC CAGTTCCTCA GTAAGCAGAA AGACTTCCAC CCCTGTCCTC
    AGCGAGGACT GCACTACCAC GGAGGAAGCA AATGAACCTG GAAACCTGCT AAACTGCAGC
    CAGAACTCCA GCTTATCCTC ATCTTGGTGG CTGAAATCAC GGGCAATCTC CAGTGGTTCT
    TCTGAGGGAG AAAATCCATG GTCTTTTCTG AATTCCAGTG GAAGTTCTTT TGTTTCATTG
    CCAGGAATGA CAAAGCAGGC GATCCTTGAG GCCCGCACCC TGCAGCCTGA TGACTTTGAA
    AAACTCTTGG CAGGGGTGAG GCATGATTGG TTGTTCCAGA GACTGGAGCA TACAGGCCTT
    TTTAAATCCA GACAACTCCA CGGAGCACAT AGTGCTCTTT TGTTAAAATA TTCCAAGAAA
    TCTGAACTGT GGACAGCCCA AGAAACTGTT GTATATTTGG GGGACTACCT ACATGTGACA
    AAGAAAGGCA GACAAAGAAA TGCATTTTGG GTACATCATC TTCATCAAGA AGAAACTCTG
    GGCAGATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC TCTGGCACCA CTTCACTGAT
    GTGGAGAGGC AGATGACCGC ACAGCACTAC GTGACAGAAT TTAACAAGAG ACTTTACGAG
    CAGAAGATTC ACACGCAGAT ATTCTACATA CCGTCCTCAG TATTGCTGAT TTTAGAAGGC
    AAGACAATAA AAGGATGTGT CAGTGTGGAA CCTTACATGC TAGGGGAATT TGTAAAATTA
    TCAAATAACA CAAAAGTGGT GAAAACAGAA TACAAAGCCA CAGAATACGG CTTGGCGTAT
    GGCCACTTTT CTTATGAATT TTCCAATCAT AGAGATGTTG TGGTTGATTT ACAAGGTTGG
    GTGACTGGGA ATGGAAAAGG GCTCATCTAC CTCACAGATC CCCAGATTCA CTCTGTTGAT
    CAGAAAGATG TCACTACCAA TTTTGGAAAG AAAGGAATTT TCTACTTCTT TAATAACCAG
    CATGTGGAAT GTAATGAAAT ATGCCATCGT CTTTCTTTGA CAAGACCTTC GATTGAGAAA
    CCAAACAATT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235499
    Clone ID Related Accession (Same CDS sequence) XM_024993506.1
    Accession Version XM_024993506.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849274.1
    CDS290..3475
    Translation

    Target ORF information:

    RefSeq Version XM_024993506.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90061
    Clone ID Related Accession (Same CDS sequence) XM_015471515.2 , XM_015471515.1 , XM_015463701.1
    Accession Version XM_015471515.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCCTG GAGTAGAAGG AGGAGGGAGC AGAATCCCAA GTAGTGGGAA TGACAAGTGC 
    AGATACCATG AAGCAAGTAC CTGGCTATAC AGAAATGAAA GCGACAAGGT CCTGGTGCAG
    TCAGTCTGCA TACAGATAAG AGGGCAAATT CTGCAGAAGT TGGGGATGTG GTATGAAGCA
    GCTGAGTTAA TATGGGCCTC AATCATGGGA TACCTGACAC TTCCTCAGCC AGACAAAAAG
    GGCATTTCCA CATCACTAGG TATATTGGCA GACATCTTCG TCTCCATGAG CAAGAAAGAT
    TATGAAAAGT TTAAAAGCAA TCCTGACATT AACCTGGGCT TGCTGAAGGA GTTTGACCAT
    CACTTGCTGT CAGCCGCTGA GGCCTGCAAG CTGGCGGCTG CCTTCAGCCC CTACACGCCC
    CTGTTCGTGC TCACGGCTAT GAATATCCGT GGCACGTGCC TCTTGTCATA TAGTAGTTCT
    GAGGACTGTC CTCCAGGAAT GAAAAATTCA TATTTATATG AAGCCAAAGA AGCCTTTGAA
    ATTGGCCTCC TCACCAAGAA ACGTGATGAA CCAGTTACTG GAAGACAGGA GCTCCATAGT
    TTTCTTAAAG CTGCTTTTGG TCTCACCACT GTGCACCAAA GACTCTATGG GGAGATGGAG
    ACCGTCCGAA CAGCAAGTCA GCTCTGTAGT GAAGCTATGG GAAAGCTGTA TACTTTCAGC
    AATTCCTCCA GAAGTCAGGA GAGAGAAGCT GTCTCTCAGG AAATCATGTC TGTTATCACC
    CGGGTGAAGG AACAGTTACA AGTTCAAAGC TTCTCAAATT TAGATGACAA GTCTTACGTT
    CCAGAGGGAT TCAAGCGTGG GTTAGAGAAG CCCATCTTAC ATGGGCAAGT GGATTTCCCA
    AAAATCCTTG AAGCCTATTC ACAGCACCAC ACTTCAGTTT GTGAACTATT TGAAAGCACT
    TGTGGGAACA ACAAAAATAA ACAGAAAGGT ATGAAAACAG GAGTCTGTAT CACAGATCTA
    AAGACAGAAA CACAAAACAC AGATACCGTG AGTGCCACGG AGGACAAAGC ACATTTTGAA
    AAAGGCAGGG TGATATCTTC TTCCCGAATG GCGATGAACA GTCAGGAGAA ACTCAGGAGG
    GCGGGAAGGA GAAACTGGGC CGGTTCTGAT GCGTTTCGAG TCTCCGTGGA TGAAGATGTG
    GAGACCGAAG CTGAGTCAAC AGACCACAGC AATGGTGGGG GAGCTGTCCT GAACAAGTCT
    CTGAGTGACA GCCTGAGCTC CAGTTCTTGG AGCCAGTTAT CAGGGTACAA ATCTTCTACA
    AGCTGGGAGG AAGTGAATTA TCATGTTGAT GATATGTCTG CCGGAAGAGA GCTGAGCAAG
    GAGGGGCATC TTGTGGACAC GCAGTGTTCC ACTGCTCTGT CAGACGAGCA GGAGGTGAAT
    GGGGTGAGCA GAGCTGAGCA CCCACTGTCT TCAGAGCTGC ATGGTCTCTC TCTCCAGGTG
    CCCAGGGATG ACAGGTTAGA GTCTTCTCAA TGTCAGCTGC ACAAGTCCAC GCCCTTGGCA
    ACCTTCCCAC CTCACAGCAC AACAGGCACT TCATTGGCCT CTGGTGGAGG GTGGTTTAAA
    GAAGGCATGC AGGAAGTTGA AAATGTGGGG TCCAGAAATA CTTCTGCTCA TTCCAGACCC
    TTATTTTGTT CTGCTTCTTG GACTTCTGAT TCTGGTTGGC CTAAGAACAT GACCACGTAT
    CCTTCCATCC AAGAAGAAGA AACCTTTGAA ACAATTGGTG AGTTTCAAGA AATCAACGGA
    GATGCCAAGG ATGGGGATGA AGAAGAGAAG AGAGAAGAAA TCAAAGGAGG CACAGGTCCT
    CCATTTAAAG ATAGCCCCTG CTGGGTTGAC CCAGAAGGAA AAATAGCAGA AAGAGCAGAA
    GATGTTCCCG TAGGCTTCCA CTCAGTCGTG GACGAGTCTC GGGACAAAAG CTGCATGGTA
    GCATGTAACT CCTACACTCC TCATTGGCTT GTTGAAAACC CTAACTCGGG AAAAAATGGT
    CTCTCAGTCA AAGAGCAGGG CATTGACCCT GATGCCTCCA CGGTGGATGA GGAGAGCCCA
    CTGCTGGGCA GCGCAGATGT TCACACCACG AGCACACGTG GCTCCCCGAG ACCGTGCACT
    TGGAAGCAGC TGCACGTTCA GGGAGCCCAG ATCCCCAGTT CCTCAGTAAG CAGAAAGACT
    TCCACCCCTG TCCTCAGCGA GGACTGCACT ACCACGGAGG AAGCAAATGA ACCTGGAAAC
    CTGCTAAACT GCAGCCAGAA CTCCAGCTTA TCCTCATCTT GGTGGCTGAA ATCACGGGCA
    ATCTCCAGTG GTTCTTCTGA GGGAGAAAAT CCATGGTCTT TTCTGAATTC CAGTGGAAGT
    TCTTTTGTTT CATTGCCAGG AATGACAAAG CAGGCGATCC TTGAGGCCCG CACCCTGCAG
    CCTGATGACT TTGAAAAACT CTTGGCAGGG GTGAGGCATG ATTGGTTGTT CCAGAGACTG
    GAGCATACAG GCCTTTTTAA ATCCAGACAA CTCCACGGAG CACATAGTGC TCTTTTGTTA
    AAATATTCCA AGAAATCTGA ACTGTGGACA GCCCAAGAAA CTGTTGTATA TTTGGGGGAC
    TACCTACATG TGACAAAGAA AGGCAGACAA AGAAATGCAT TTTGGGTACA TCATCTTCAT
    CAAGAAGAAA CTCTGGGCAG ATATGTTGGG AAAGAATATA AGGAGCAAAA GGGGCTCTGG
    CACCACTTCA CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAATTTAAC
    AAGAGACTTT ACGAGCAGAA GATTCACACG CAGATATTCT ACATACCGTC CTCAGTATTG
    CTGATTTTAG AAGGCAAGAC AATAAAAGGA TGTGTCAGTG TGGAACCTTA CATGCTAGGG
    GAATTTGTAA AATTATCAAA TAACACAAAA GTGGTGAAAA CAGAATACAA AGCCACAGAA
    TACGGCTTGG CGTATGGCCA CTTTTCTTAT GAATTTTCCA ATCATAGAGA TGTTGTGGTT
    GATTTACAAG GTTGGGTGAC TGGGAATGGA AAAGGGCTCA TCTACCTCAC AGATCCCCAG
    ATTCACTCTG TTGATCAGAA AGATGTCACT ACCAATTTTG GAAAGAAAGG AATTTTCTAC
    TTCTTTAATA ACCAGCATGT GGAATGTAAT GAAATATGCC ATCGTCTTTC TTTGACAAGA
    CCTTCGATTG AGAAACCAAA CAATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327001.1
    CDS109..3378
    Translation

    Target ORF information:

    RefSeq Version XM_015471515.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471515.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCCTG GAGTAGAAGG AGGAGGGAGC AGAATCCCAA GTAGTGGGAA TGACAAGTGC 
    AGATACCATG AAGCAAGTAC CTGGCTATAC AGAAATGAAA GCGACAAGGT CCTGGTGCAG
    TCAGTCTGCA TACAGATAAG AGGGCAAATT CTGCAGAAGT TGGGGATGTG GTATGAAGCA
    GCTGAGTTAA TATGGGCCTC AATCATGGGA TACCTGACAC TTCCTCAGCC AGACAAAAAG
    GGCATTTCCA CATCACTAGG TATATTGGCA GACATCTTCG TCTCCATGAG CAAGAAAGAT
    TATGAAAAGT TTAAAAGCAA TCCTGACATT AACCTGGGCT TGCTGAAGGA GTTTGACCAT
    CACTTGCTGT CAGCCGCTGA GGCCTGCAAG CTGGCGGCTG CCTTCAGCCC CTACACGCCC
    CTGTTCGTGC TCACGGCTAT GAATATCCGT GGCACGTGCC TCTTGTCATA TAGTAGTTCT
    GAGGACTGTC CTCCAGGAAT GAAAAATTCA TATTTATATG AAGCCAAAGA AGCCTTTGAA
    ATTGGCCTCC TCACCAAGAA ACGTGATGAA CCAGTTACTG GAAGACAGGA GCTCCATAGT
    TTTCTTAAAG CTGCTTTTGG TCTCACCACT GTGCACCAAA GACTCTATGG GGAGATGGAG
    ACCGTCCGAA CAGCAAGTCA GCTCTGTAGT GAAGCTATGG GAAAGCTGTA TACTTTCAGC
    AATTCCTCCA GAAGTCAGGA GAGAGAAGCT GTCTCTCAGG AAATCATGTC TGTTATCACC
    CGGGTGAAGG AACAGTTACA AGTTCAAAGC TTCTCAAATT TAGATGACAA GTCTTACGTT
    CCAGAGGGAT TCAAGCGTGG GTTAGAGAAG CCCATCTTAC ATGGGCAAGT GGATTTCCCA
    AAAATCCTTG AAGCCTATTC ACAGCACCAC ACTTCAGTTT GTGAACTATT TGAAAGCACT
    TGTGGGAACA ACAAAAATAA ACAGAAAGGT ATGAAAACAG GAGTCTGTAT CACAGATCTA
    AAGACAGAAA CACAAAACAC AGATACCGTG AGTGCCACGG AGGACAAAGC ACATTTTGAA
    AAAGGCAGGG TGATATCTTC TTCCCGAATG GCGATGAACA GTCAGGAGAA ACTCAGGAGG
    GCGGGAAGGA GAAACTGGGC CGGTTCTGAT GCGTTTCGAG TCTCCGTGGA TGAAGATGTG
    GAGACCGAAG CTGAGTCAAC AGACCACAGC AATGGTGGGG GAGCTGTCCT GAACAAGTCT
    CTGAGTGACA GCCTGAGCTC CAGTTCTTGG AGCCAGTTAT CAGGGTACAA ATCTTCTACA
    AGCTGGGAGG AAGTGAATTA TCATGTTGAT GATATGTCTG CCGGAAGAGA GCTGAGCAAG
    GAGGGGCATC TTGTGGACAC GCAGTGTTCC ACTGCTCTGT CAGACGAGCA GGAGGTGAAT
    GGGGTGAGCA GAGCTGAGCA CCCACTGTCT TCAGAGCTGC ATGGTCTCTC TCTCCAGGTG
    CCCAGGGATG ACAGGTTAGA GTCTTCTCAA TGTCAGCTGC ACAAGTCCAC GCCCTTGGCA
    ACCTTCCCAC CTCACAGCAC AACAGGCACT TCATTGGCCT CTGGTGGAGG GTGGTTTAAA
    GAAGGCATGC AGGAAGTTGA AAATGTGGGG TCCAGAAATA CTTCTGCTCA TTCCAGACCC
    TTATTTTGTT CTGCTTCTTG GACTTCTGAT TCTGGTTGGC CTAAGAACAT GACCACGTAT
    CCTTCCATCC AAGAAGAAGA AACCTTTGAA ACAATTGGTG AGTTTCAAGA AATCAACGGA
    GATGCCAAGG ATGGGGATGA AGAAGAGAAG AGAGAAGAAA TCAAAGGAGG CACAGGTCCT
    CCATTTAAAG ATAGCCCCTG CTGGGTTGAC CCAGAAGGAA AAATAGCAGA AAGAGCAGAA
    GATGTTCCCG TAGGCTTCCA CTCAGTCGTG GACGAGTCTC GGGACAAAAG CTGCATGGTA
    GCATGTAACT CCTACACTCC TCATTGGCTT GTTGAAAACC CTAACTCGGG AAAAAATGGT
    CTCTCAGTCA AAGAGCAGGG CATTGACCCT GATGCCTCCA CGGTGGATGA GGAGAGCCCA
    CTGCTGGGCA GCGCAGATGT TCACACCACG AGCACACGTG GCTCCCCGAG ACCGTGCACT
    TGGAAGCAGC TGCACGTTCA GGGAGCCCAG ATCCCCAGTT CCTCAGTAAG CAGAAAGACT
    TCCACCCCTG TCCTCAGCGA GGACTGCACT ACCACGGAGG AAGCAAATGA ACCTGGAAAC
    CTGCTAAACT GCAGCCAGAA CTCCAGCTTA TCCTCATCTT GGTGGCTGAA ATCACGGGCA
    ATCTCCAGTG GTTCTTCTGA GGGAGAAAAT CCATGGTCTT TTCTGAATTC CAGTGGAAGT
    TCTTTTGTTT CATTGCCAGG AATGACAAAG CAGGCGATCC TTGAGGCCCG CACCCTGCAG
    CCTGATGACT TTGAAAAACT CTTGGCAGGG GTGAGGCATG ATTGGTTGTT CCAGAGACTG
    GAGCATACAG GCCTTTTTAA ATCCAGACAA CTCCACGGAG CACATAGTGC TCTTTTGTTA
    AAATATTCCA AGAAATCTGA ACTGTGGACA GCCCAAGAAA CTGTTGTATA TTTGGGGGAC
    TACCTACATG TGACAAAGAA AGGCAGACAA AGAAATGCAT TTTGGGTACA TCATCTTCAT
    CAAGAAGAAA CTCTGGGCAG ATATGTTGGG AAAGAATATA AGGAGCAAAA GGGGCTCTGG
    CACCACTTCA CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAATTTAAC
    AAGAGACTTT ACGAGCAGAA GATTCACACG CAGATATTCT ACATACCGTC CTCAGTATTG
    CTGATTTTAG AAGGCAAGAC AATAAAAGGA TGTGTCAGTG TGGAACCTTA CATGCTAGGG
    GAATTTGTAA AATTATCAAA TAACACAAAA GTGGTGAAAA CAGAATACAA AGCCACAGAA
    TACGGCTTGG CGTATGGCCA CTTTTCTTAT GAATTTTCCA ATCATAGAGA TGTTGTGGTT
    GATTTACAAG GTTGGGTGAC TGGGAATGGA AAAGGGCTCA TCTACCTCAC AGATCCCCAG
    ATTCACTCTG TTGATCAGAA AGATGTCACT ACCAATTTTG GAAAGAAAGG AATTTTCTAC
    TTCTTTAATA ACCAGCATGT GGAATGTAAT GAAATATGCC ATCGTCTTTC TTTGACAAGA
    CCTTCGATTG AGAAACCAAA CAATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90061
    Clone ID Related Accession (Same CDS sequence) XM_015471515.2 , XM_015471515.1 , XM_015463701.1
    Accession Version XM_015471515.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCCTG GAGTAGAAGG AGGAGGGAGC AGAATCCCAA GTAGTGGGAA TGACAAGTGC 
    AGATACCATG AAGCAAGTAC CTGGCTATAC AGAAATGAAA GCGACAAGGT CCTGGTGCAG
    TCAGTCTGCA TACAGATAAG AGGGCAAATT CTGCAGAAGT TGGGGATGTG GTATGAAGCA
    GCTGAGTTAA TATGGGCCTC AATCATGGGA TACCTGACAC TTCCTCAGCC AGACAAAAAG
    GGCATTTCCA CATCACTAGG TATATTGGCA GACATCTTCG TCTCCATGAG CAAGAAAGAT
    TATGAAAAGT TTAAAAGCAA TCCTGACATT AACCTGGGCT TGCTGAAGGA GTTTGACCAT
    CACTTGCTGT CAGCCGCTGA GGCCTGCAAG CTGGCGGCTG CCTTCAGCCC CTACACGCCC
    CTGTTCGTGC TCACGGCTAT GAATATCCGT GGCACGTGCC TCTTGTCATA TAGTAGTTCT
    GAGGACTGTC CTCCAGGAAT GAAAAATTCA TATTTATATG AAGCCAAAGA AGCCTTTGAA
    ATTGGCCTCC TCACCAAGAA ACGTGATGAA CCAGTTACTG GAAGACAGGA GCTCCATAGT
    TTTCTTAAAG CTGCTTTTGG TCTCACCACT GTGCACCAAA GACTCTATGG GGAGATGGAG
    ACCGTCCGAA CAGCAAGTCA GCTCTGTAGT GAAGCTATGG GAAAGCTGTA TACTTTCAGC
    AATTCCTCCA GAAGTCAGGA GAGAGAAGCT GTCTCTCAGG AAATCATGTC TGTTATCACC
    CGGGTGAAGG AACAGTTACA AGTTCAAAGC TTCTCAAATT TAGATGACAA GTCTTACGTT
    CCAGAGGGAT TCAAGCGTGG GTTAGAGAAG CCCATCTTAC ATGGGCAAGT GGATTTCCCA
    AAAATCCTTG AAGCCTATTC ACAGCACCAC ACTTCAGTTT GTGAACTATT TGAAAGCACT
    TGTGGGAACA ACAAAAATAA ACAGAAAGGT ATGAAAACAG GAGTCTGTAT CACAGATCTA
    AAGACAGAAA CACAAAACAC AGATACCGTG AGTGCCACGG AGGACAAAGC ACATTTTGAA
    AAAGGCAGGG TGATATCTTC TTCCCGAATG GCGATGAACA GTCAGGAGAA ACTCAGGAGG
    GCGGGAAGGA GAAACTGGGC CGGTTCTGAT GCGTTTCGAG TCTCCGTGGA TGAAGATGTG
    GAGACCGAAG CTGAGTCAAC AGACCACAGC AATGGTGGGG GAGCTGTCCT GAACAAGTCT
    CTGAGTGACA GCCTGAGCTC CAGTTCTTGG AGCCAGTTAT CAGGGTACAA ATCTTCTACA
    AGCTGGGAGG AAGTGAATTA TCATGTTGAT GATATGTCTG CCGGAAGAGA GCTGAGCAAG
    GAGGGGCATC TTGTGGACAC GCAGTGTTCC ACTGCTCTGT CAGACGAGCA GGAGGTGAAT
    GGGGTGAGCA GAGCTGAGCA CCCACTGTCT TCAGAGCTGC ATGGTCTCTC TCTCCAGGTG
    CCCAGGGATG ACAGGTTAGA GTCTTCTCAA TGTCAGCTGC ACAAGTCCAC GCCCTTGGCA
    ACCTTCCCAC CTCACAGCAC AACAGGCACT TCATTGGCCT CTGGTGGAGG GTGGTTTAAA
    GAAGGCATGC AGGAAGTTGA AAATGTGGGG TCCAGAAATA CTTCTGCTCA TTCCAGACCC
    TTATTTTGTT CTGCTTCTTG GACTTCTGAT TCTGGTTGGC CTAAGAACAT GACCACGTAT
    CCTTCCATCC AAGAAGAAGA AACCTTTGAA ACAATTGGTG AGTTTCAAGA AATCAACGGA
    GATGCCAAGG ATGGGGATGA AGAAGAGAAG AGAGAAGAAA TCAAAGGAGG CACAGGTCCT
    CCATTTAAAG ATAGCCCCTG CTGGGTTGAC CCAGAAGGAA AAATAGCAGA AAGAGCAGAA
    GATGTTCCCG TAGGCTTCCA CTCAGTCGTG GACGAGTCTC GGGACAAAAG CTGCATGGTA
    GCATGTAACT CCTACACTCC TCATTGGCTT GTTGAAAACC CTAACTCGGG AAAAAATGGT
    CTCTCAGTCA AAGAGCAGGG CATTGACCCT GATGCCTCCA CGGTGGATGA GGAGAGCCCA
    CTGCTGGGCA GCGCAGATGT TCACACCACG AGCACACGTG GCTCCCCGAG ACCGTGCACT
    TGGAAGCAGC TGCACGTTCA GGGAGCCCAG ATCCCCAGTT CCTCAGTAAG CAGAAAGACT
    TCCACCCCTG TCCTCAGCGA GGACTGCACT ACCACGGAGG AAGCAAATGA ACCTGGAAAC
    CTGCTAAACT GCAGCCAGAA CTCCAGCTTA TCCTCATCTT GGTGGCTGAA ATCACGGGCA
    ATCTCCAGTG GTTCTTCTGA GGGAGAAAAT CCATGGTCTT TTCTGAATTC CAGTGGAAGT
    TCTTTTGTTT CATTGCCAGG AATGACAAAG CAGGCGATCC TTGAGGCCCG CACCCTGCAG
    CCTGATGACT TTGAAAAACT CTTGGCAGGG GTGAGGCATG ATTGGTTGTT CCAGAGACTG
    GAGCATACAG GCCTTTTTAA ATCCAGACAA CTCCACGGAG CACATAGTGC TCTTTTGTTA
    AAATATTCCA AGAAATCTGA ACTGTGGACA GCCCAAGAAA CTGTTGTATA TTTGGGGGAC
    TACCTACATG TGACAAAGAA AGGCAGACAA AGAAATGCAT TTTGGGTACA TCATCTTCAT
    CAAGAAGAAA CTCTGGGCAG ATATGTTGGG AAAGAATATA AGGAGCAAAA GGGGCTCTGG
    CACCACTTCA CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAATTTAAC
    AAGAGACTTT ACGAGCAGAA GATTCACACG CAGATATTCT ACATACCGTC CTCAGTATTG
    CTGATTTTAG AAGGCAAGAC AATAAAAGGA TGTGTCAGTG TGGAACCTTA CATGCTAGGG
    GAATTTGTAA AATTATCAAA TAACACAAAA GTGGTGAAAA CAGAATACAA AGCCACAGAA
    TACGGCTTGG CGTATGGCCA CTTTTCTTAT GAATTTTCCA ATCATAGAGA TGTTGTGGTT
    GATTTACAAG GTTGGGTGAC TGGGAATGGA AAAGGGCTCA TCTACCTCAC AGATCCCCAG
    ATTCACTCTG TTGATCAGAA AGATGTCACT ACCAATTTTG GAAAGAAAGG AATTTTCTAC
    TTCTTTAATA ACCAGCATGT GGAATGTAAT GAAATATGCC ATCGTCTTTC TTTGACAAGA
    CCTTCGATTG AGAAACCAAA CAATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327001.1
    CDS102..3371
    Translation

    Target ORF information:

    RefSeq Version XM_015471515.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471515.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCCTG GAGTAGAAGG AGGAGGGAGC AGAATCCCAA GTAGTGGGAA TGACAAGTGC 
    AGATACCATG AAGCAAGTAC CTGGCTATAC AGAAATGAAA GCGACAAGGT CCTGGTGCAG
    TCAGTCTGCA TACAGATAAG AGGGCAAATT CTGCAGAAGT TGGGGATGTG GTATGAAGCA
    GCTGAGTTAA TATGGGCCTC AATCATGGGA TACCTGACAC TTCCTCAGCC AGACAAAAAG
    GGCATTTCCA CATCACTAGG TATATTGGCA GACATCTTCG TCTCCATGAG CAAGAAAGAT
    TATGAAAAGT TTAAAAGCAA TCCTGACATT AACCTGGGCT TGCTGAAGGA GTTTGACCAT
    CACTTGCTGT CAGCCGCTGA GGCCTGCAAG CTGGCGGCTG CCTTCAGCCC CTACACGCCC
    CTGTTCGTGC TCACGGCTAT GAATATCCGT GGCACGTGCC TCTTGTCATA TAGTAGTTCT
    GAGGACTGTC CTCCAGGAAT GAAAAATTCA TATTTATATG AAGCCAAAGA AGCCTTTGAA
    ATTGGCCTCC TCACCAAGAA ACGTGATGAA CCAGTTACTG GAAGACAGGA GCTCCATAGT
    TTTCTTAAAG CTGCTTTTGG TCTCACCACT GTGCACCAAA GACTCTATGG GGAGATGGAG
    ACCGTCCGAA CAGCAAGTCA GCTCTGTAGT GAAGCTATGG GAAAGCTGTA TACTTTCAGC
    AATTCCTCCA GAAGTCAGGA GAGAGAAGCT GTCTCTCAGG AAATCATGTC TGTTATCACC
    CGGGTGAAGG AACAGTTACA AGTTCAAAGC TTCTCAAATT TAGATGACAA GTCTTACGTT
    CCAGAGGGAT TCAAGCGTGG GTTAGAGAAG CCCATCTTAC ATGGGCAAGT GGATTTCCCA
    AAAATCCTTG AAGCCTATTC ACAGCACCAC ACTTCAGTTT GTGAACTATT TGAAAGCACT
    TGTGGGAACA ACAAAAATAA ACAGAAAGGT ATGAAAACAG GAGTCTGTAT CACAGATCTA
    AAGACAGAAA CACAAAACAC AGATACCGTG AGTGCCACGG AGGACAAAGC ACATTTTGAA
    AAAGGCAGGG TGATATCTTC TTCCCGAATG GCGATGAACA GTCAGGAGAA ACTCAGGAGG
    GCGGGAAGGA GAAACTGGGC CGGTTCTGAT GCGTTTCGAG TCTCCGTGGA TGAAGATGTG
    GAGACCGAAG CTGAGTCAAC AGACCACAGC AATGGTGGGG GAGCTGTCCT GAACAAGTCT
    CTGAGTGACA GCCTGAGCTC CAGTTCTTGG AGCCAGTTAT CAGGGTACAA ATCTTCTACA
    AGCTGGGAGG AAGTGAATTA TCATGTTGAT GATATGTCTG CCGGAAGAGA GCTGAGCAAG
    GAGGGGCATC TTGTGGACAC GCAGTGTTCC ACTGCTCTGT CAGACGAGCA GGAGGTGAAT
    GGGGTGAGCA GAGCTGAGCA CCCACTGTCT TCAGAGCTGC ATGGTCTCTC TCTCCAGGTG
    CCCAGGGATG ACAGGTTAGA GTCTTCTCAA TGTCAGCTGC ACAAGTCCAC GCCCTTGGCA
    ACCTTCCCAC CTCACAGCAC AACAGGCACT TCATTGGCCT CTGGTGGAGG GTGGTTTAAA
    GAAGGCATGC AGGAAGTTGA AAATGTGGGG TCCAGAAATA CTTCTGCTCA TTCCAGACCC
    TTATTTTGTT CTGCTTCTTG GACTTCTGAT TCTGGTTGGC CTAAGAACAT GACCACGTAT
    CCTTCCATCC AAGAAGAAGA AACCTTTGAA ACAATTGGTG AGTTTCAAGA AATCAACGGA
    GATGCCAAGG ATGGGGATGA AGAAGAGAAG AGAGAAGAAA TCAAAGGAGG CACAGGTCCT
    CCATTTAAAG ATAGCCCCTG CTGGGTTGAC CCAGAAGGAA AAATAGCAGA AAGAGCAGAA
    GATGTTCCCG TAGGCTTCCA CTCAGTCGTG GACGAGTCTC GGGACAAAAG CTGCATGGTA
    GCATGTAACT CCTACACTCC TCATTGGCTT GTTGAAAACC CTAACTCGGG AAAAAATGGT
    CTCTCAGTCA AAGAGCAGGG CATTGACCCT GATGCCTCCA CGGTGGATGA GGAGAGCCCA
    CTGCTGGGCA GCGCAGATGT TCACACCACG AGCACACGTG GCTCCCCGAG ACCGTGCACT
    TGGAAGCAGC TGCACGTTCA GGGAGCCCAG ATCCCCAGTT CCTCAGTAAG CAGAAAGACT
    TCCACCCCTG TCCTCAGCGA GGACTGCACT ACCACGGAGG AAGCAAATGA ACCTGGAAAC
    CTGCTAAACT GCAGCCAGAA CTCCAGCTTA TCCTCATCTT GGTGGCTGAA ATCACGGGCA
    ATCTCCAGTG GTTCTTCTGA GGGAGAAAAT CCATGGTCTT TTCTGAATTC CAGTGGAAGT
    TCTTTTGTTT CATTGCCAGG AATGACAAAG CAGGCGATCC TTGAGGCCCG CACCCTGCAG
    CCTGATGACT TTGAAAAACT CTTGGCAGGG GTGAGGCATG ATTGGTTGTT CCAGAGACTG
    GAGCATACAG GCCTTTTTAA ATCCAGACAA CTCCACGGAG CACATAGTGC TCTTTTGTTA
    AAATATTCCA AGAAATCTGA ACTGTGGACA GCCCAAGAAA CTGTTGTATA TTTGGGGGAC
    TACCTACATG TGACAAAGAA AGGCAGACAA AGAAATGCAT TTTGGGTACA TCATCTTCAT
    CAAGAAGAAA CTCTGGGCAG ATATGTTGGG AAAGAATATA AGGAGCAAAA GGGGCTCTGG
    CACCACTTCA CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAATTTAAC
    AAGAGACTTT ACGAGCAGAA GATTCACACG CAGATATTCT ACATACCGTC CTCAGTATTG
    CTGATTTTAG AAGGCAAGAC AATAAAAGGA TGTGTCAGTG TGGAACCTTA CATGCTAGGG
    GAATTTGTAA AATTATCAAA TAACACAAAA GTGGTGAAAA CAGAATACAA AGCCACAGAA
    TACGGCTTGG CGTATGGCCA CTTTTCTTAT GAATTTTCCA ATCATAGAGA TGTTGTGGTT
    GATTTACAAG GTTGGGTGAC TGGGAATGGA AAAGGGCTCA TCTACCTCAC AGATCCCCAG
    ATTCACTCTG TTGATCAGAA AGATGTCACT ACCAATTTTG GAAAGAAAGG AATTTTCTAC
    TTCTTTAATA ACCAGCATGT GGAATGTAAT GAAATATGCC ATCGTCTTTC TTTGACAAGA
    CCTTCGATTG AGAAACCAAA CAATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90061
    Clone ID Related Accession (Same CDS sequence) XM_015471515.2 , XM_015471515.1 , XM_015463701.1
    Accession Version XM_015463701.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCCTG GAGTAGAAGG AGGAGGGAGC AGAATCCCAA GTAGTGGGAA TGACAAGTGC 
    AGATACCATG AAGCAAGTAC CTGGCTATAC AGAAATGAAA GCGACAAGGT CCTGGTGCAG
    TCAGTCTGCA TACAGATAAG AGGGCAAATT CTGCAGAAGT TGGGGATGTG GTATGAAGCA
    GCTGAGTTAA TATGGGCCTC AATCATGGGA TACCTGACAC TTCCTCAGCC AGACAAAAAG
    GGCATTTCCA CATCACTAGG TATATTGGCA GACATCTTCG TCTCCATGAG CAAGAAAGAT
    TATGAAAAGT TTAAAAGCAA TCCTGACATT AACCTGGGCT TGCTGAAGGA GTTTGACCAT
    CACTTGCTGT CAGCCGCTGA GGCCTGCAAG CTGGCGGCTG CCTTCAGCCC CTACACGCCC
    CTGTTCGTGC TCACGGCTAT GAATATCCGT GGCACGTGCC TCTTGTCATA TAGTAGTTCT
    GAGGACTGTC CTCCAGGAAT GAAAAATTCA TATTTATATG AAGCCAAAGA AGCCTTTGAA
    ATTGGCCTCC TCACCAAGAA ACGTGATGAA CCAGTTACTG GAAGACAGGA GCTCCATAGT
    TTTCTTAAAG CTGCTTTTGG TCTCACCACT GTGCACCAAA GACTCTATGG GGAGATGGAG
    ACCGTCCGAA CAGCAAGTCA GCTCTGTAGT GAAGCTATGG GAAAGCTGTA TACTTTCAGC
    AATTCCTCCA GAAGTCAGGA GAGAGAAGCT GTCTCTCAGG AAATCATGTC TGTTATCACC
    CGGGTGAAGG AACAGTTACA AGTTCAAAGC TTCTCAAATT TAGATGACAA GTCTTACGTT
    CCAGAGGGAT TCAAGCGTGG GTTAGAGAAG CCCATCTTAC ATGGGCAAGT GGATTTCCCA
    AAAATCCTTG AAGCCTATTC ACAGCACCAC ACTTCAGTTT GTGAACTATT TGAAAGCACT
    TGTGGGAACA ACAAAAATAA ACAGAAAGGT ATGAAAACAG GAGTCTGTAT CACAGATCTA
    AAGACAGAAA CACAAAACAC AGATACCGTG AGTGCCACGG AGGACAAAGC ACATTTTGAA
    AAAGGCAGGG TGATATCTTC TTCCCGAATG GCGATGAACA GTCAGGAGAA ACTCAGGAGG
    GCGGGAAGGA GAAACTGGGC CGGTTCTGAT GCGTTTCGAG TCTCCGTGGA TGAAGATGTG
    GAGACCGAAG CTGAGTCAAC AGACCACAGC AATGGTGGGG GAGCTGTCCT GAACAAGTCT
    CTGAGTGACA GCCTGAGCTC CAGTTCTTGG AGCCAGTTAT CAGGGTACAA ATCTTCTACA
    AGCTGGGAGG AAGTGAATTA TCATGTTGAT GATATGTCTG CCGGAAGAGA GCTGAGCAAG
    GAGGGGCATC TTGTGGACAC GCAGTGTTCC ACTGCTCTGT CAGACGAGCA GGAGGTGAAT
    GGGGTGAGCA GAGCTGAGCA CCCACTGTCT TCAGAGCTGC ATGGTCTCTC TCTCCAGGTG
    CCCAGGGATG ACAGGTTAGA GTCTTCTCAA TGTCAGCTGC ACAAGTCCAC GCCCTTGGCA
    ACCTTCCCAC CTCACAGCAC AACAGGCACT TCATTGGCCT CTGGTGGAGG GTGGTTTAAA
    GAAGGCATGC AGGAAGTTGA AAATGTGGGG TCCAGAAATA CTTCTGCTCA TTCCAGACCC
    TTATTTTGTT CTGCTTCTTG GACTTCTGAT TCTGGTTGGC CTAAGAACAT GACCACGTAT
    CCTTCCATCC AAGAAGAAGA AACCTTTGAA ACAATTGGTG AGTTTCAAGA AATCAACGGA
    GATGCCAAGG ATGGGGATGA AGAAGAGAAG AGAGAAGAAA TCAAAGGAGG CACAGGTCCT
    CCATTTAAAG ATAGCCCCTG CTGGGTTGAC CCAGAAGGAA AAATAGCAGA AAGAGCAGAA
    GATGTTCCCG TAGGCTTCCA CTCAGTCGTG GACGAGTCTC GGGACAAAAG CTGCATGGTA
    GCATGTAACT CCTACACTCC TCATTGGCTT GTTGAAAACC CTAACTCGGG AAAAAATGGT
    CTCTCAGTCA AAGAGCAGGG CATTGACCCT GATGCCTCCA CGGTGGATGA GGAGAGCCCA
    CTGCTGGGCA GCGCAGATGT TCACACCACG AGCACACGTG GCTCCCCGAG ACCGTGCACT
    TGGAAGCAGC TGCACGTTCA GGGAGCCCAG ATCCCCAGTT CCTCAGTAAG CAGAAAGACT
    TCCACCCCTG TCCTCAGCGA GGACTGCACT ACCACGGAGG AAGCAAATGA ACCTGGAAAC
    CTGCTAAACT GCAGCCAGAA CTCCAGCTTA TCCTCATCTT GGTGGCTGAA ATCACGGGCA
    ATCTCCAGTG GTTCTTCTGA GGGAGAAAAT CCATGGTCTT TTCTGAATTC CAGTGGAAGT
    TCTTTTGTTT CATTGCCAGG AATGACAAAG CAGGCGATCC TTGAGGCCCG CACCCTGCAG
    CCTGATGACT TTGAAAAACT CTTGGCAGGG GTGAGGCATG ATTGGTTGTT CCAGAGACTG
    GAGCATACAG GCCTTTTTAA ATCCAGACAA CTCCACGGAG CACATAGTGC TCTTTTGTTA
    AAATATTCCA AGAAATCTGA ACTGTGGACA GCCCAAGAAA CTGTTGTATA TTTGGGGGAC
    TACCTACATG TGACAAAGAA AGGCAGACAA AGAAATGCAT TTTGGGTACA TCATCTTCAT
    CAAGAAGAAA CTCTGGGCAG ATATGTTGGG AAAGAATATA AGGAGCAAAA GGGGCTCTGG
    CACCACTTCA CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAATTTAAC
    AAGAGACTTT ACGAGCAGAA GATTCACACG CAGATATTCT ACATACCGTC CTCAGTATTG
    CTGATTTTAG AAGGCAAGAC AATAAAAGGA TGTGTCAGTG TGGAACCTTA CATGCTAGGG
    GAATTTGTAA AATTATCAAA TAACACAAAA GTGGTGAAAA CAGAATACAA AGCCACAGAA
    TACGGCTTGG CGTATGGCCA CTTTTCTTAT GAATTTTCCA ATCATAGAGA TGTTGTGGTT
    GATTTACAAG GTTGGGTGAC TGGGAATGGA AAAGGGCTCA TCTACCTCAC AGATCCCCAG
    ATTCACTCTG TTGATCAGAA AGATGTCACT ACCAATTTTG GAAAGAAAGG AATTTTCTAC
    TTCTTTAATA ACCAGCATGT GGAATGTAAT GAAATATGCC ATCGTCTTTC TTTGACAAGA
    CCTTCGATTG AGAAACCAAA CAATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319187.1
    CDS102..3371
    Translation

    Target ORF information:

    RefSeq Version XM_015463701.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463701.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGAGCCCTG GAGTAGAAGG AGGAGGGAGC AGAATCCCAA GTAGTGGGAA TGACAAGTGC 
    AGATACCATG AAGCAAGTAC CTGGCTATAC AGAAATGAAA GCGACAAGGT CCTGGTGCAG
    TCAGTCTGCA TACAGATAAG AGGGCAAATT CTGCAGAAGT TGGGGATGTG GTATGAAGCA
    GCTGAGTTAA TATGGGCCTC AATCATGGGA TACCTGACAC TTCCTCAGCC AGACAAAAAG
    GGCATTTCCA CATCACTAGG TATATTGGCA GACATCTTCG TCTCCATGAG CAAGAAAGAT
    TATGAAAAGT TTAAAAGCAA TCCTGACATT AACCTGGGCT TGCTGAAGGA GTTTGACCAT
    CACTTGCTGT CAGCCGCTGA GGCCTGCAAG CTGGCGGCTG CCTTCAGCCC CTACACGCCC
    CTGTTCGTGC TCACGGCTAT GAATATCCGT GGCACGTGCC TCTTGTCATA TAGTAGTTCT
    GAGGACTGTC CTCCAGGAAT GAAAAATTCA TATTTATATG AAGCCAAAGA AGCCTTTGAA
    ATTGGCCTCC TCACCAAGAA ACGTGATGAA CCAGTTACTG GAAGACAGGA GCTCCATAGT
    TTTCTTAAAG CTGCTTTTGG TCTCACCACT GTGCACCAAA GACTCTATGG GGAGATGGAG
    ACCGTCCGAA CAGCAAGTCA GCTCTGTAGT GAAGCTATGG GAAAGCTGTA TACTTTCAGC
    AATTCCTCCA GAAGTCAGGA GAGAGAAGCT GTCTCTCAGG AAATCATGTC TGTTATCACC
    CGGGTGAAGG AACAGTTACA AGTTCAAAGC TTCTCAAATT TAGATGACAA GTCTTACGTT
    CCAGAGGGAT TCAAGCGTGG GTTAGAGAAG CCCATCTTAC ATGGGCAAGT GGATTTCCCA
    AAAATCCTTG AAGCCTATTC ACAGCACCAC ACTTCAGTTT GTGAACTATT TGAAAGCACT
    TGTGGGAACA ACAAAAATAA ACAGAAAGGT ATGAAAACAG GAGTCTGTAT CACAGATCTA
    AAGACAGAAA CACAAAACAC AGATACCGTG AGTGCCACGG AGGACAAAGC ACATTTTGAA
    AAAGGCAGGG TGATATCTTC TTCCCGAATG GCGATGAACA GTCAGGAGAA ACTCAGGAGG
    GCGGGAAGGA GAAACTGGGC CGGTTCTGAT GCGTTTCGAG TCTCCGTGGA TGAAGATGTG
    GAGACCGAAG CTGAGTCAAC AGACCACAGC AATGGTGGGG GAGCTGTCCT GAACAAGTCT
    CTGAGTGACA GCCTGAGCTC CAGTTCTTGG AGCCAGTTAT CAGGGTACAA ATCTTCTACA
    AGCTGGGAGG AAGTGAATTA TCATGTTGAT GATATGTCTG CCGGAAGAGA GCTGAGCAAG
    GAGGGGCATC TTGTGGACAC GCAGTGTTCC ACTGCTCTGT CAGACGAGCA GGAGGTGAAT
    GGGGTGAGCA GAGCTGAGCA CCCACTGTCT TCAGAGCTGC ATGGTCTCTC TCTCCAGGTG
    CCCAGGGATG ACAGGTTAGA GTCTTCTCAA TGTCAGCTGC ACAAGTCCAC GCCCTTGGCA
    ACCTTCCCAC CTCACAGCAC AACAGGCACT TCATTGGCCT CTGGTGGAGG GTGGTTTAAA
    GAAGGCATGC AGGAAGTTGA AAATGTGGGG TCCAGAAATA CTTCTGCTCA TTCCAGACCC
    TTATTTTGTT CTGCTTCTTG GACTTCTGAT TCTGGTTGGC CTAAGAACAT GACCACGTAT
    CCTTCCATCC AAGAAGAAGA AACCTTTGAA ACAATTGGTG AGTTTCAAGA AATCAACGGA
    GATGCCAAGG ATGGGGATGA AGAAGAGAAG AGAGAAGAAA TCAAAGGAGG CACAGGTCCT
    CCATTTAAAG ATAGCCCCTG CTGGGTTGAC CCAGAAGGAA AAATAGCAGA AAGAGCAGAA
    GATGTTCCCG TAGGCTTCCA CTCAGTCGTG GACGAGTCTC GGGACAAAAG CTGCATGGTA
    GCATGTAACT CCTACACTCC TCATTGGCTT GTTGAAAACC CTAACTCGGG AAAAAATGGT
    CTCTCAGTCA AAGAGCAGGG CATTGACCCT GATGCCTCCA CGGTGGATGA GGAGAGCCCA
    CTGCTGGGCA GCGCAGATGT TCACACCACG AGCACACGTG GCTCCCCGAG ACCGTGCACT
    TGGAAGCAGC TGCACGTTCA GGGAGCCCAG ATCCCCAGTT CCTCAGTAAG CAGAAAGACT
    TCCACCCCTG TCCTCAGCGA GGACTGCACT ACCACGGAGG AAGCAAATGA ACCTGGAAAC
    CTGCTAAACT GCAGCCAGAA CTCCAGCTTA TCCTCATCTT GGTGGCTGAA ATCACGGGCA
    ATCTCCAGTG GTTCTTCTGA GGGAGAAAAT CCATGGTCTT TTCTGAATTC CAGTGGAAGT
    TCTTTTGTTT CATTGCCAGG AATGACAAAG CAGGCGATCC TTGAGGCCCG CACCCTGCAG
    CCTGATGACT TTGAAAAACT CTTGGCAGGG GTGAGGCATG ATTGGTTGTT CCAGAGACTG
    GAGCATACAG GCCTTTTTAA ATCCAGACAA CTCCACGGAG CACATAGTGC TCTTTTGTTA
    AAATATTCCA AGAAATCTGA ACTGTGGACA GCCCAAGAAA CTGTTGTATA TTTGGGGGAC
    TACCTACATG TGACAAAGAA AGGCAGACAA AGAAATGCAT TTTGGGTACA TCATCTTCAT
    CAAGAAGAAA CTCTGGGCAG ATATGTTGGG AAAGAATATA AGGAGCAAAA GGGGCTCTGG
    CACCACTTCA CTGATGTGGA GAGGCAGATG ACCGCACAGC ACTACGTGAC AGAATTTAAC
    AAGAGACTTT ACGAGCAGAA GATTCACACG CAGATATTCT ACATACCGTC CTCAGTATTG
    CTGATTTTAG AAGGCAAGAC AATAAAAGGA TGTGTCAGTG TGGAACCTTA CATGCTAGGG
    GAATTTGTAA AATTATCAAA TAACACAAAA GTGGTGAAAA CAGAATACAA AGCCACAGAA
    TACGGCTTGG CGTATGGCCA CTTTTCTTAT GAATTTTCCA ATCATAGAGA TGTTGTGGTT
    GATTTACAAG GTTGGGTGAC TGGGAATGGA AAAGGGCTCA TCTACCTCAC AGATCCCCAG
    ATTCACTCTG TTGATCAGAA AGATGTCACT ACCAATTTTG GAAAGAAAGG AATTTTCTAC
    TTCTTTAATA ACCAGCATGT GGAATGTAAT GAAATATGCC ATCGTCTTTC TTTGACAAGA
    CCTTCGATTG AGAAACCAAA CAATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_015463693.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319179.1
    CDS321..4049
    Translation

    Target ORF information:

    RefSeq Version XM_015463693.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_015463696.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319182.1
    CDS405..4133
    Translation

    Target ORF information:

    RefSeq Version XM_015463696.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463696.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_005207667.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207724.1
    CDS269..3997
    Translation

    Target ORF information:

    RefSeq Version XM_005207667.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207667.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_015463694.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319180.1
    CDS269..3997
    Translation

    Target ORF information:

    RefSeq Version XM_015463694.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463694.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_010805922.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804224.1
    CDS321..4049
    Translation

    Target ORF information:

    RefSeq Version XM_010805922.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805922.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_010805923.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804225.1
    CDS141..3869
    Translation

    Target ORF information:

    RefSeq Version XM_010805923.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805923.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_005207668.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207725.1
    CDS92..3820
    Translation

    Target ORF information:

    RefSeq Version XM_005207668.3
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207668.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_015463695.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319181.1
    CDS141..3869
    Translation

    Target ORF information:

    RefSeq Version XM_015463695.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463695.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_015463697.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319183.1
    CDS92..3820
    Translation

    Target ORF information:

    RefSeq Version XM_015463697.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463697.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20201
    Clone ID Related Accession (Same CDS sequence) XM_015463693.1 , XM_015463696.1 , XM_005207667.3 , XM_015463694.1 , XM_010805922.2 , XM_010805923.2 , XM_005207668.3 , XM_015463695.1 , XM_015463697.1 , XM_010805924.2
    Accession Version XM_010805924.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804226.1
    CDS405..4133
    Translation

    Target ORF information:

    RefSeq Version XM_010805924.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805924.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20202
    Clone ID Related Accession (Same CDS sequence) XM_015471513.1 , XM_015463699.1
    Accession Version XM_015471513.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGGATG
    TGGTATGAAG CAGCTGAGTT AATATGGGCC TCAATCATGG GATACCTGAC ACTTCCTCAG
    CCAGACAAAA AGGGCATTTC CACATCACTA GGTATATTGG CAGACATCTT CGTCTCCATG
    AGCAAGAAAG ATTATGAAAA GTTTAAAAGC AATCCTGACA TTAACCTGGG CTTGCTGAAG
    GAGTTTGACC ATCACTTGCT GTCAGCCGCT GAGGCCTGCA AGCTGGCGGC TGCCTTCAGC
    CCCTACACGC CCCTGTTCGT GCTCACGGCT ATGAATATCC GTGGCACGTG CCTCTTGTCA
    TATAGTAGTT CTGAGGACTG TCCTCCAGGA ATGAAAAATT CATATTTATA TGAAGCCAAA
    GAAGCCTTTG AAATTGGCCT CCTCACCAAG AAACGTGATG AACCAGTTAC TGGAAGACAG
    GAGCTCCATA GTTTTCTTAA AGCTGCTTTT GGTCTCACCA CTGTGCACCA AAGACTCTAT
    GGGGAGATGG AGACCGTCCG AACAGCAAGT CAGCTCTGTA GTGAAGCTAT GGGAAAGCTG
    TATACTTTCA GCAATTCCTC CAGAAGTCAG GAGAGAGAAG CTGTCTCTCA GGAAATCATG
    TCTGTTATCA CCCGGGTGAA GGAACAGTTA CAAGTTCAAA GCTTCTCAAA TTTAGATGAC
    AAGTCTTACG TTCCAGAGGG ATTCAAGCGT GGGTTAGAGA AGCCCATCTT ACATGGGCAA
    GTGGATTTCC CAAAAATCCT TGAAGCCTAT TCACAGCACC ACACTTCAGT TTGTGAACTA
    TTTGAAAGCA CTTGTGGGAA CAACAAAAAT AAACAGAAAG GTATGAAAAC AGGAGTCTGT
    ATCACAGATC TAAAGACAGA AACACAAAAC ACAGATACCG TGAGTGCCAC GGAGGACAAA
    GCACATTTTG AAAAAGGCAG GGTGATATCT TCTTCCCGAA TGGCGATGAA CAGTCAGGAG
    AAACTCAGGA GGGCGGGAAG GAGAAACTGG GCCGGTTCTG ATGCGTTTCG AGTCTCCGTG
    GATGAAGATG TGGAGACCGA AGCTGAGTCA ACAGACCACA GCAATGGTGG GGGAGCTGTC
    CTGAACAAGT CTCTGAGTGA CAGCCTGAGC TCCAGTTCTT GGAGCCAGTT ATCAGGGTAC
    AAATCTTCTA CAAGCTGGGA GGAAGTGAAT TATCATGTTG ATGATATGTC TGCCGGAAGA
    GAGCTGAGCA AGGAGGGGCA TCTTGTGGAC ACGCAGTGTT CCACTGCTCT GTCAGACGAG
    CAGGAGGTGA ATGGGGTGAG CAGAGCTGAG CACCCACTGT CTTCAGAGCT GCATGGTCTC
    TCTCTCCAGG TGCCCAGGGA TGACAGGTTA GAGTCTTCTC AATGTCAGCT GCACAAGTCC
    ACGCCCTTGG CAACCTTCCC ACCTCACAGC ACAACAGGCA CTTCATTGGC CTCTGGTGGA
    GGGTGGTTTA AAGAAGGCAT GCAGGAAGTT GAAAATGTGG GGTCCAGAAA TACTTCTGCT
    CATTCCAGAC CCTTATTTTG TTCTGCTTCT TGGACTTCTG ATTCTGGTTG GCCTAAGAAC
    ATGACCACGT ATCCTTCCAT CCAAGAAGAA GAAACCTTTG AAACAATTGG TGAGTTTCAA
    GAAATCAACG GAGATGCCAA GGATGGGGAT GAAGAAGAGA AGAGAGAAGA AATCAAAGGA
    GGCACAGGTC CTCCATTTAA AGATAGCCCC TGCTGGGTTG ACCCAGAAGG AAAAATAGCA
    GAAAGAGCAG AAGATGTTCC CGTAGGCTTC CACTCAGTCG TGGACGAGTC TCGGGACAAA
    AGCTGCATGG TAGCATGTAA CTCCTACACT CCTCATTGGC TTGTTGAAAA CCCTAACTCG
    GGAAAAAATG GTCTCTCAGT CAAAGAGCAG GGCATTGACC CTGATGCCTC CACGGTGGAT
    GAGGAGAGCC CACTGCTGGG CAGCGCAGAT GTTCACACCA CGAGCACACG TGGCTCCCCG
    AGACCGTGCA CTTGGAAGCA GCTGCACGTT CAGGGAGCCC AGATCCCCAG TTCCTCAGTA
    AGCAGAAAGA CTTCCACCCC TGTCCTCAGC GAGGACTGCA CTACCACGGA GGAAGCAAAT
    GAACCTGGAA ACCTGCTAAA CTGCAGCCAG AACTCCAGCT TATCCTCATC TTGGTGGCTG
    AAATCACGGG CAATCTCCAG TGGTTCTTCT GAGGGAGAAA ATCCATGGTC TTTTCTGAAT
    TCCAGTGGAA GTTCTTTTGT TTCATTGCCA GGAATGACAA AGCAGGCGAT CCTTGAGGCC
    CGCACCCTGC AGCCTGATGA CTTTGAAAAA CTCTTGGCAG GGGTGAGGCA TGATTGGTTG
    TTCCAGAGAC TGGAGCATAC AGGCCTTTTT AAATCCAGAC AACTCCACGG AGCACATAGT
    GCTCTTTTGT TAAAATATTC CAAGAAATCT GAACTGTGGA CAGCCCAAGA AACTGTTGTA
    TATTTGGGGG ACTACCTACA TGTGACAAAG AAAGGCAGAC AAAGAAATGC ATTTTGGGTA
    CATCATCTTC ATCAAGAAGA AACTCTGGGC AGATATGTTG GGAAAGAATA TAAGGAGCAA
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGAGGCAGA TGACCGCACA GCACTACGTG
    ACAGAATTTA ACAAGAGACT TTACGAGCAG AAGATTCACA CGCAGATATT CTACATACCG
    TCCTCAGTAT TGCTGATTTT AGAAGGCAAG ACAATAAAAG GATGTGTCAG TGTGGAACCT
    TACATGCTAG GGGAATTTGT AAAATTATCA AATAACACAA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATACGGCTT GGCGTATGGC CACTTTTCTT ATGAATTTTC CAATCATAGA
    GATGTTGTGG TTGATTTACA AGGTTGGGTG ACTGGGAATG GAAAAGGGCT CATCTACCTC
    ACAGATCCCC AGATTCACTC TGTTGATCAG AAAGATGTCA CTACCAATTT TGGAAAGAAA
    GGAATTTTCT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATATG CCATCGTCTT
    TCTTTGACAA GACCTTCGAT TGAGAAACCA AACAATTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326999.1
    CDS322..3963
    Translation

    Target ORF information:

    RefSeq Version XM_015471513.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471513.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGGATG
    TGGTATGAAG CAGCTGAGTT AATATGGGCC TCAATCATGG GATACCTGAC ACTTCCTCAG
    CCAGACAAAA AGGGCATTTC CACATCACTA GGTATATTGG CAGACATCTT CGTCTCCATG
    AGCAAGAAAG ATTATGAAAA GTTTAAAAGC AATCCTGACA TTAACCTGGG CTTGCTGAAG
    GAGTTTGACC ATCACTTGCT GTCAGCCGCT GAGGCCTGCA AGCTGGCGGC TGCCTTCAGC
    CCCTACACGC CCCTGTTCGT GCTCACGGCT ATGAATATCC GTGGCACGTG CCTCTTGTCA
    TATAGTAGTT CTGAGGACTG TCCTCCAGGA ATGAAAAATT CATATTTATA TGAAGCCAAA
    GAAGCCTTTG AAATTGGCCT CCTCACCAAG AAACGTGATG AACCAGTTAC TGGAAGACAG
    GAGCTCCATA GTTTTCTTAA AGCTGCTTTT GGTCTCACCA CTGTGCACCA AAGACTCTAT
    GGGGAGATGG AGACCGTCCG AACAGCAAGT CAGCTCTGTA GTGAAGCTAT GGGAAAGCTG
    TATACTTTCA GCAATTCCTC CAGAAGTCAG GAGAGAGAAG CTGTCTCTCA GGAAATCATG
    TCTGTTATCA CCCGGGTGAA GGAACAGTTA CAAGTTCAAA GCTTCTCAAA TTTAGATGAC
    AAGTCTTACG TTCCAGAGGG ATTCAAGCGT GGGTTAGAGA AGCCCATCTT ACATGGGCAA
    GTGGATTTCC CAAAAATCCT TGAAGCCTAT TCACAGCACC ACACTTCAGT TTGTGAACTA
    TTTGAAAGCA CTTGTGGGAA CAACAAAAAT AAACAGAAAG GTATGAAAAC AGGAGTCTGT
    ATCACAGATC TAAAGACAGA AACACAAAAC ACAGATACCG TGAGTGCCAC GGAGGACAAA
    GCACATTTTG AAAAAGGCAG GGTGATATCT TCTTCCCGAA TGGCGATGAA CAGTCAGGAG
    AAACTCAGGA GGGCGGGAAG GAGAAACTGG GCCGGTTCTG ATGCGTTTCG AGTCTCCGTG
    GATGAAGATG TGGAGACCGA AGCTGAGTCA ACAGACCACA GCAATGGTGG GGGAGCTGTC
    CTGAACAAGT CTCTGAGTGA CAGCCTGAGC TCCAGTTCTT GGAGCCAGTT ATCAGGGTAC
    AAATCTTCTA CAAGCTGGGA GGAAGTGAAT TATCATGTTG ATGATATGTC TGCCGGAAGA
    GAGCTGAGCA AGGAGGGGCA TCTTGTGGAC ACGCAGTGTT CCACTGCTCT GTCAGACGAG
    CAGGAGGTGA ATGGGGTGAG CAGAGCTGAG CACCCACTGT CTTCAGAGCT GCATGGTCTC
    TCTCTCCAGG TGCCCAGGGA TGACAGGTTA GAGTCTTCTC AATGTCAGCT GCACAAGTCC
    ACGCCCTTGG CAACCTTCCC ACCTCACAGC ACAACAGGCA CTTCATTGGC CTCTGGTGGA
    GGGTGGTTTA AAGAAGGCAT GCAGGAAGTT GAAAATGTGG GGTCCAGAAA TACTTCTGCT
    CATTCCAGAC CCTTATTTTG TTCTGCTTCT TGGACTTCTG ATTCTGGTTG GCCTAAGAAC
    ATGACCACGT ATCCTTCCAT CCAAGAAGAA GAAACCTTTG AAACAATTGG TGAGTTTCAA
    GAAATCAACG GAGATGCCAA GGATGGGGAT GAAGAAGAGA AGAGAGAAGA AATCAAAGGA
    GGCACAGGTC CTCCATTTAA AGATAGCCCC TGCTGGGTTG ACCCAGAAGG AAAAATAGCA
    GAAAGAGCAG AAGATGTTCC CGTAGGCTTC CACTCAGTCG TGGACGAGTC TCGGGACAAA
    AGCTGCATGG TAGCATGTAA CTCCTACACT CCTCATTGGC TTGTTGAAAA CCCTAACTCG
    GGAAAAAATG GTCTCTCAGT CAAAGAGCAG GGCATTGACC CTGATGCCTC CACGGTGGAT
    GAGGAGAGCC CACTGCTGGG CAGCGCAGAT GTTCACACCA CGAGCACACG TGGCTCCCCG
    AGACCGTGCA CTTGGAAGCA GCTGCACGTT CAGGGAGCCC AGATCCCCAG TTCCTCAGTA
    AGCAGAAAGA CTTCCACCCC TGTCCTCAGC GAGGACTGCA CTACCACGGA GGAAGCAAAT
    GAACCTGGAA ACCTGCTAAA CTGCAGCCAG AACTCCAGCT TATCCTCATC TTGGTGGCTG
    AAATCACGGG CAATCTCCAG TGGTTCTTCT GAGGGAGAAA ATCCATGGTC TTTTCTGAAT
    TCCAGTGGAA GTTCTTTTGT TTCATTGCCA GGAATGACAA AGCAGGCGAT CCTTGAGGCC
    CGCACCCTGC AGCCTGATGA CTTTGAAAAA CTCTTGGCAG GGGTGAGGCA TGATTGGTTG
    TTCCAGAGAC TGGAGCATAC AGGCCTTTTT AAATCCAGAC AACTCCACGG AGCACATAGT
    GCTCTTTTGT TAAAATATTC CAAGAAATCT GAACTGTGGA CAGCCCAAGA AACTGTTGTA
    TATTTGGGGG ACTACCTACA TGTGACAAAG AAAGGCAGAC AAAGAAATGC ATTTTGGGTA
    CATCATCTTC ATCAAGAAGA AACTCTGGGC AGATATGTTG GGAAAGAATA TAAGGAGCAA
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGAGGCAGA TGACCGCACA GCACTACGTG
    ACAGAATTTA ACAAGAGACT TTACGAGCAG AAGATTCACA CGCAGATATT CTACATACCG
    TCCTCAGTAT TGCTGATTTT AGAAGGCAAG ACAATAAAAG GATGTGTCAG TGTGGAACCT
    TACATGCTAG GGGAATTTGT AAAATTATCA AATAACACAA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATACGGCTT GGCGTATGGC CACTTTTCTT ATGAATTTTC CAATCATAGA
    GATGTTGTGG TTGATTTACA AGGTTGGGTG ACTGGGAATG GAAAAGGGCT CATCTACCTC
    ACAGATCCCC AGATTCACTC TGTTGATCAG AAAGATGTCA CTACCAATTT TGGAAAGAAA
    GGAATTTTCT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATATG CCATCGTCTT
    TCTTTGACAA GACCTTCGAT TGAGAAACCA AACAATTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20202
    Clone ID Related Accession (Same CDS sequence) XM_015471513.1 , XM_015463699.1
    Accession Version XM_015463699.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGGATG
    TGGTATGAAG CAGCTGAGTT AATATGGGCC TCAATCATGG GATACCTGAC ACTTCCTCAG
    CCAGACAAAA AGGGCATTTC CACATCACTA GGTATATTGG CAGACATCTT CGTCTCCATG
    AGCAAGAAAG ATTATGAAAA GTTTAAAAGC AATCCTGACA TTAACCTGGG CTTGCTGAAG
    GAGTTTGACC ATCACTTGCT GTCAGCCGCT GAGGCCTGCA AGCTGGCGGC TGCCTTCAGC
    CCCTACACGC CCCTGTTCGT GCTCACGGCT ATGAATATCC GTGGCACGTG CCTCTTGTCA
    TATAGTAGTT CTGAGGACTG TCCTCCAGGA ATGAAAAATT CATATTTATA TGAAGCCAAA
    GAAGCCTTTG AAATTGGCCT CCTCACCAAG AAACGTGATG AACCAGTTAC TGGAAGACAG
    GAGCTCCATA GTTTTCTTAA AGCTGCTTTT GGTCTCACCA CTGTGCACCA AAGACTCTAT
    GGGGAGATGG AGACCGTCCG AACAGCAAGT CAGCTCTGTA GTGAAGCTAT GGGAAAGCTG
    TATACTTTCA GCAATTCCTC CAGAAGTCAG GAGAGAGAAG CTGTCTCTCA GGAAATCATG
    TCTGTTATCA CCCGGGTGAA GGAACAGTTA CAAGTTCAAA GCTTCTCAAA TTTAGATGAC
    AAGTCTTACG TTCCAGAGGG ATTCAAGCGT GGGTTAGAGA AGCCCATCTT ACATGGGCAA
    GTGGATTTCC CAAAAATCCT TGAAGCCTAT TCACAGCACC ACACTTCAGT TTGTGAACTA
    TTTGAAAGCA CTTGTGGGAA CAACAAAAAT AAACAGAAAG GTATGAAAAC AGGAGTCTGT
    ATCACAGATC TAAAGACAGA AACACAAAAC ACAGATACCG TGAGTGCCAC GGAGGACAAA
    GCACATTTTG AAAAAGGCAG GGTGATATCT TCTTCCCGAA TGGCGATGAA CAGTCAGGAG
    AAACTCAGGA GGGCGGGAAG GAGAAACTGG GCCGGTTCTG ATGCGTTTCG AGTCTCCGTG
    GATGAAGATG TGGAGACCGA AGCTGAGTCA ACAGACCACA GCAATGGTGG GGGAGCTGTC
    CTGAACAAGT CTCTGAGTGA CAGCCTGAGC TCCAGTTCTT GGAGCCAGTT ATCAGGGTAC
    AAATCTTCTA CAAGCTGGGA GGAAGTGAAT TATCATGTTG ATGATATGTC TGCCGGAAGA
    GAGCTGAGCA AGGAGGGGCA TCTTGTGGAC ACGCAGTGTT CCACTGCTCT GTCAGACGAG
    CAGGAGGTGA ATGGGGTGAG CAGAGCTGAG CACCCACTGT CTTCAGAGCT GCATGGTCTC
    TCTCTCCAGG TGCCCAGGGA TGACAGGTTA GAGTCTTCTC AATGTCAGCT GCACAAGTCC
    ACGCCCTTGG CAACCTTCCC ACCTCACAGC ACAACAGGCA CTTCATTGGC CTCTGGTGGA
    GGGTGGTTTA AAGAAGGCAT GCAGGAAGTT GAAAATGTGG GGTCCAGAAA TACTTCTGCT
    CATTCCAGAC CCTTATTTTG TTCTGCTTCT TGGACTTCTG ATTCTGGTTG GCCTAAGAAC
    ATGACCACGT ATCCTTCCAT CCAAGAAGAA GAAACCTTTG AAACAATTGG TGAGTTTCAA
    GAAATCAACG GAGATGCCAA GGATGGGGAT GAAGAAGAGA AGAGAGAAGA AATCAAAGGA
    GGCACAGGTC CTCCATTTAA AGATAGCCCC TGCTGGGTTG ACCCAGAAGG AAAAATAGCA
    GAAAGAGCAG AAGATGTTCC CGTAGGCTTC CACTCAGTCG TGGACGAGTC TCGGGACAAA
    AGCTGCATGG TAGCATGTAA CTCCTACACT CCTCATTGGC TTGTTGAAAA CCCTAACTCG
    GGAAAAAATG GTCTCTCAGT CAAAGAGCAG GGCATTGACC CTGATGCCTC CACGGTGGAT
    GAGGAGAGCC CACTGCTGGG CAGCGCAGAT GTTCACACCA CGAGCACACG TGGCTCCCCG
    AGACCGTGCA CTTGGAAGCA GCTGCACGTT CAGGGAGCCC AGATCCCCAG TTCCTCAGTA
    AGCAGAAAGA CTTCCACCCC TGTCCTCAGC GAGGACTGCA CTACCACGGA GGAAGCAAAT
    GAACCTGGAA ACCTGCTAAA CTGCAGCCAG AACTCCAGCT TATCCTCATC TTGGTGGCTG
    AAATCACGGG CAATCTCCAG TGGTTCTTCT GAGGGAGAAA ATCCATGGTC TTTTCTGAAT
    TCCAGTGGAA GTTCTTTTGT TTCATTGCCA GGAATGACAA AGCAGGCGAT CCTTGAGGCC
    CGCACCCTGC AGCCTGATGA CTTTGAAAAA CTCTTGGCAG GGGTGAGGCA TGATTGGTTG
    TTCCAGAGAC TGGAGCATAC AGGCCTTTTT AAATCCAGAC AACTCCACGG AGCACATAGT
    GCTCTTTTGT TAAAATATTC CAAGAAATCT GAACTGTGGA CAGCCCAAGA AACTGTTGTA
    TATTTGGGGG ACTACCTACA TGTGACAAAG AAAGGCAGAC AAAGAAATGC ATTTTGGGTA
    CATCATCTTC ATCAAGAAGA AACTCTGGGC AGATATGTTG GGAAAGAATA TAAGGAGCAA
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGAGGCAGA TGACCGCACA GCACTACGTG
    ACAGAATTTA ACAAGAGACT TTACGAGCAG AAGATTCACA CGCAGATATT CTACATACCG
    TCCTCAGTAT TGCTGATTTT AGAAGGCAAG ACAATAAAAG GATGTGTCAG TGTGGAACCT
    TACATGCTAG GGGAATTTGT AAAATTATCA AATAACACAA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATACGGCTT GGCGTATGGC CACTTTTCTT ATGAATTTTC CAATCATAGA
    GATGTTGTGG TTGATTTACA AGGTTGGGTG ACTGGGAATG GAAAAGGGCT CATCTACCTC
    ACAGATCCCC AGATTCACTC TGTTGATCAG AAAGATGTCA CTACCAATTT TGGAAAGAAA
    GGAATTTTCT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATATG CCATCGTCTT
    TCTTTGACAA GACCTTCGAT TGAGAAACCA AACAATTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319185.1
    CDS322..3963
    Translation

    Target ORF information:

    RefSeq Version XM_015463699.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463699.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGGATG
    TGGTATGAAG CAGCTGAGTT AATATGGGCC TCAATCATGG GATACCTGAC ACTTCCTCAG
    CCAGACAAAA AGGGCATTTC CACATCACTA GGTATATTGG CAGACATCTT CGTCTCCATG
    AGCAAGAAAG ATTATGAAAA GTTTAAAAGC AATCCTGACA TTAACCTGGG CTTGCTGAAG
    GAGTTTGACC ATCACTTGCT GTCAGCCGCT GAGGCCTGCA AGCTGGCGGC TGCCTTCAGC
    CCCTACACGC CCCTGTTCGT GCTCACGGCT ATGAATATCC GTGGCACGTG CCTCTTGTCA
    TATAGTAGTT CTGAGGACTG TCCTCCAGGA ATGAAAAATT CATATTTATA TGAAGCCAAA
    GAAGCCTTTG AAATTGGCCT CCTCACCAAG AAACGTGATG AACCAGTTAC TGGAAGACAG
    GAGCTCCATA GTTTTCTTAA AGCTGCTTTT GGTCTCACCA CTGTGCACCA AAGACTCTAT
    GGGGAGATGG AGACCGTCCG AACAGCAAGT CAGCTCTGTA GTGAAGCTAT GGGAAAGCTG
    TATACTTTCA GCAATTCCTC CAGAAGTCAG GAGAGAGAAG CTGTCTCTCA GGAAATCATG
    TCTGTTATCA CCCGGGTGAA GGAACAGTTA CAAGTTCAAA GCTTCTCAAA TTTAGATGAC
    AAGTCTTACG TTCCAGAGGG ATTCAAGCGT GGGTTAGAGA AGCCCATCTT ACATGGGCAA
    GTGGATTTCC CAAAAATCCT TGAAGCCTAT TCACAGCACC ACACTTCAGT TTGTGAACTA
    TTTGAAAGCA CTTGTGGGAA CAACAAAAAT AAACAGAAAG GTATGAAAAC AGGAGTCTGT
    ATCACAGATC TAAAGACAGA AACACAAAAC ACAGATACCG TGAGTGCCAC GGAGGACAAA
    GCACATTTTG AAAAAGGCAG GGTGATATCT TCTTCCCGAA TGGCGATGAA CAGTCAGGAG
    AAACTCAGGA GGGCGGGAAG GAGAAACTGG GCCGGTTCTG ATGCGTTTCG AGTCTCCGTG
    GATGAAGATG TGGAGACCGA AGCTGAGTCA ACAGACCACA GCAATGGTGG GGGAGCTGTC
    CTGAACAAGT CTCTGAGTGA CAGCCTGAGC TCCAGTTCTT GGAGCCAGTT ATCAGGGTAC
    AAATCTTCTA CAAGCTGGGA GGAAGTGAAT TATCATGTTG ATGATATGTC TGCCGGAAGA
    GAGCTGAGCA AGGAGGGGCA TCTTGTGGAC ACGCAGTGTT CCACTGCTCT GTCAGACGAG
    CAGGAGGTGA ATGGGGTGAG CAGAGCTGAG CACCCACTGT CTTCAGAGCT GCATGGTCTC
    TCTCTCCAGG TGCCCAGGGA TGACAGGTTA GAGTCTTCTC AATGTCAGCT GCACAAGTCC
    ACGCCCTTGG CAACCTTCCC ACCTCACAGC ACAACAGGCA CTTCATTGGC CTCTGGTGGA
    GGGTGGTTTA AAGAAGGCAT GCAGGAAGTT GAAAATGTGG GGTCCAGAAA TACTTCTGCT
    CATTCCAGAC CCTTATTTTG TTCTGCTTCT TGGACTTCTG ATTCTGGTTG GCCTAAGAAC
    ATGACCACGT ATCCTTCCAT CCAAGAAGAA GAAACCTTTG AAACAATTGG TGAGTTTCAA
    GAAATCAACG GAGATGCCAA GGATGGGGAT GAAGAAGAGA AGAGAGAAGA AATCAAAGGA
    GGCACAGGTC CTCCATTTAA AGATAGCCCC TGCTGGGTTG ACCCAGAAGG AAAAATAGCA
    GAAAGAGCAG AAGATGTTCC CGTAGGCTTC CACTCAGTCG TGGACGAGTC TCGGGACAAA
    AGCTGCATGG TAGCATGTAA CTCCTACACT CCTCATTGGC TTGTTGAAAA CCCTAACTCG
    GGAAAAAATG GTCTCTCAGT CAAAGAGCAG GGCATTGACC CTGATGCCTC CACGGTGGAT
    GAGGAGAGCC CACTGCTGGG CAGCGCAGAT GTTCACACCA CGAGCACACG TGGCTCCCCG
    AGACCGTGCA CTTGGAAGCA GCTGCACGTT CAGGGAGCCC AGATCCCCAG TTCCTCAGTA
    AGCAGAAAGA CTTCCACCCC TGTCCTCAGC GAGGACTGCA CTACCACGGA GGAAGCAAAT
    GAACCTGGAA ACCTGCTAAA CTGCAGCCAG AACTCCAGCT TATCCTCATC TTGGTGGCTG
    AAATCACGGG CAATCTCCAG TGGTTCTTCT GAGGGAGAAA ATCCATGGTC TTTTCTGAAT
    TCCAGTGGAA GTTCTTTTGT TTCATTGCCA GGAATGACAA AGCAGGCGAT CCTTGAGGCC
    CGCACCCTGC AGCCTGATGA CTTTGAAAAA CTCTTGGCAG GGGTGAGGCA TGATTGGTTG
    TTCCAGAGAC TGGAGCATAC AGGCCTTTTT AAATCCAGAC AACTCCACGG AGCACATAGT
    GCTCTTTTGT TAAAATATTC CAAGAAATCT GAACTGTGGA CAGCCCAAGA AACTGTTGTA
    TATTTGGGGG ACTACCTACA TGTGACAAAG AAAGGCAGAC AAAGAAATGC ATTTTGGGTA
    CATCATCTTC ATCAAGAAGA AACTCTGGGC AGATATGTTG GGAAAGAATA TAAGGAGCAA
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGAGGCAGA TGACCGCACA GCACTACGTG
    ACAGAATTTA ACAAGAGACT TTACGAGCAG AAGATTCACA CGCAGATATT CTACATACCG
    TCCTCAGTAT TGCTGATTTT AGAAGGCAAG ACAATAAAAG GATGTGTCAG TGTGGAACCT
    TACATGCTAG GGGAATTTGT AAAATTATCA AATAACACAA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATACGGCTT GGCGTATGGC CACTTTTCTT ATGAATTTTC CAATCATAGA
    GATGTTGTGG TTGATTTACA AGGTTGGGTG ACTGGGAATG GAAAAGGGCT CATCTACCTC
    ACAGATCCCC AGATTCACTC TGTTGATCAG AAAGATGTCA CTACCAATTT TGGAAAGAAA
    GGAATTTTCT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATATG CCATCGTCTT
    TCTTTGACAA GACCTTCGAT TGAGAAACCA AACAATTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20203
    Clone ID Related Accession (Same CDS sequence) XM_015471514.1 , XM_015463700.1
    Accession Version XM_015471514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCAGA GGAGGACAAG AGGGAGGACC AGAGATGCAG AGGCGTCCCT GAAAGCCTCC 
    ATCCTCGCCC GGGACTGCGC CACGGCCGCC GCCATCGTGT TCCTGAGCGA CCGTCTCCTG
    TACGGGCTCG ACGTCTCGGG CCAGCTGCTG CAGGTCGCCA AAGCGCTGCA CAGGCTGCAG
    CCCGCGACGC CCATCGCCCC GCAGGTGGTC ATCCGCCAGG CCCGAATCTC GATGCATGCA
    GGGAAACTTT TAAAAGCAGA GTATATCCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TATACAGAAA TGAAAGCGAC AAGGTCCTGG TGCAGTCAGT CTGCATACAG
    ATAAGAGGGC AAATTCTGCA GAAGTTGGGG ATGTGGTATG AAGCAGCTGA GTTAATATGG
    GCCTCAATCA TGGGATACCT GACACTTCCT CAGCCAGACA AAAAGGGCAT TTCCACATCA
    CTAGGTATAT TGGCAGACAT CTTCGTCTCC ATGAGCAAGA AAGATTATGA AAAGTTTAAA
    AGCAATCCTG ACATTAACCT GGGCTTGCTG AAGGAGTTTG ACCATCACTT GCTGTCAGCC
    GCTGAGGCCT GCAAGCTGGC GGCTGCCTTC AGCCCCTACA CGCCCCTGTT CGTGCTCACG
    GCTATGAATA TCCGTGGCAC GTGCCTCTTG TCATATAGTA GTTCTGAGGA CTGTCCTCCA
    GGAATGAAAA ATTCATATTT ATATGAAGCC AAAGAAGCCT TTGAAATTGG CCTCCTCACC
    AAGAAACGTG ATGAACCAGT TACTGGAAGA CAGGAGCTCC ATAGTTTTCT TAAAGCTGCT
    TTTGGTCTCA CCACTGTGCA CCAAAGACTC TATGGGGAGA TGGAGACCGT CCGAACAGCA
    AGTCAGCTCT GTAGTGAAGC TATGGGAAAG CTGTATACTT TCAGCAATTC CTCCAGAAGT
    CAGGAGAGAG AAGCTGTCTC TCAGGAAATC ATGTCTGTTA TCACCCGGGT GAAGGAACAG
    TTACAAGTTC AAAGCTTCTC AAATTTAGAT GACAAGTCTT ACGTTCCAGA GGGATTCAAG
    CGTGGGTTAG AGAAGCCCAT CTTACATGGG CAAGTGGATT TCCCAAAAAT CCTTGAAGCC
    TATTCACAGC ACCACACTTC AGTTTGTGAA CTATTTGAAA GCACTTGTGG GAACAACAAA
    AATAAACAGA AAGGTATGAA AACAGGAGTC TGTATCACAG ATCTAAAGAC AGAAACACAA
    AACACAGATA CCGTGAGTGC CACGGAGGAC AAAGCACATT TTGAAAAAGG CAGGGTGATA
    TCTTCTTCCC GAATGGCGAT GAACAGTCAG GAGAAACTCA GGAGGGCGGG AAGGAGAAAC
    TGGGCCGGTT CTGATGCGTT TCGAGTCTCC GTGGATGAAG ATGTGGAGAC CGAAGCTGAG
    TCAACAGACC ACAGCAATGG TGGGGGAGCT GTCCTGAACA AGTCTCTGAG TGACAGCCTG
    AGCTCCAGTT CTTGGAGCCA GTTATCAGGG TACAAATCTT CTACAAGCTG GGAGGAAGTG
    AATTATCATG TTGATGATAT GTCTGCCGGA AGAGAGCTGA GCAAGGAGGG GCATCTTGTG
    GACACGCAGT GTTCCACTGC TCTGTCAGAC GAGCAGGAGG TGAATGGGGT GAGCAGAGCT
    GAGCACCCAC TGTCTTCAGA GCTGCATGGT CTCTCTCTCC AGGTGCCCAG GGATGACAGG
    TTAGAGTCTT CTCAATGTCA GCTGCACAAG TCCACGCCCT TGGCAACCTT CCCACCTCAC
    AGCACAACAG GCACTTCATT GGCCTCTGGT GGAGGGTGGT TTAAAGAAGG CATGCAGGAA
    GTTGAAAATG TGGGGTCCAG AAATACTTCT GCTCATTCCA GACCCTTATT TTGTTCTGCT
    TCTTGGACTT CTGATTCTGG TTGGCCTAAG AACATGACCA CGTATCCTTC CATCCAAGAA
    GAAGAAACCT TTGAAACAAT TGGTGAGTTT CAAGAAATCA ACGGAGATGC CAAGGATGGG
    GATGAAGAAG AGAAGAGAGA AGAAATCAAA GGAGGCACAG GTCCTCCATT TAAAGATAGC
    CCCTGCTGGG TTGACCCAGA AGGAAAAATA GCAGAAAGAG CAGAAGATGT TCCCGTAGGC
    TTCCACTCAG TCGTGGACGA GTCTCGGGAC AAAAGCTGCA TGGTAGCATG TAACTCCTAC
    ACTCCTCATT GGCTTGTTGA AAACCCTAAC TCGGGAAAAA ATGGTCTCTC AGTCAAAGAG
    CAGGGCATTG ACCCTGATGC CTCCACGGTG GATGAGGAGA GCCCACTGCT GGGCAGCGCA
    GATGTTCACA CCACGAGCAC ACGTGGCTCC CCGAGACCGT GCACTTGGAA GCAGCTGCAC
    GTTCAGGGAG CCCAGATCCC CAGTTCCTCA GTAAGCAGAA AGACTTCCAC CCCTGTCCTC
    AGCGAGGACT GCACTACCAC GGAGGAAGCA AATGAACCTG GAAACCTGCT AAACTGCAGC
    CAGAACTCCA GCTTATCCTC ATCTTGGTGG CTGAAATCAC GGGCAATCTC CAGTGGTTCT
    TCTGAGGGAG AAAATCCATG GTCTTTTCTG AATTCCAGTG GAAGTTCTTT TGTTTCATTG
    CCAGGAATGA CAAAGCAGGC GATCCTTGAG GCCCGCACCC TGCAGCCTGA TGACTTTGAA
    AAACTCTTGG CAGGGGTGAG GCATGATTGG TTGTTCCAGA GACTGGAGCA TACAGGCCTT
    TTTAAATCCA GACAACTCCA CGGAGCACAT AGTGCTCTTT TGTTAAAATA TTCCAAGAAA
    TCTGAACTGT GGACAGCCCA AGAAACTGTT GTATATTTGG GGGACTACCT ACATGTGACA
    AAGAAAGGCA GACAAAGAAA TGCATTTTGG GTACATCATC TTCATCAAGA AGAAACTCTG
    GGCAGATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC TCTGGCACCA CTTCACTGAT
    GTGGAGAGGC AGATGACCGC ACAGCACTAC GTGACAGAAT TTAACAAGAG ACTTTACGAG
    CAGAAGATTC ACACGCAGAT ATTCTACATA CCGTCCTCAG TATTGCTGAT TTTAGAAGGC
    AAGACAATAA AAGGATGTGT CAGTGTGGAA CCTTACATGC TAGGGGAATT TGTAAAATTA
    TCAAATAACA CAAAAGTGGT GAAAACAGAA TACAAAGCCA CAGAATACGG CTTGGCGTAT
    GGCCACTTTT CTTATGAATT TTCCAATCAT AGAGATGTTG TGGTTGATTT ACAAGGTTGG
    GTGACTGGGA ATGGAAAAGG GCTCATCTAC CTCACAGATC CCCAGATTCA CTCTGTTGAT
    CAGAAAGATG TCACTACCAA TTTTGGAAAG AAAGGAATTT TCTACTTCTT TAATAACCAG
    CATGTGGAAT GTAATGAAAT ATGCCATCGT CTTTCTTTGA CAAGACCTTC GATTGAGAAA
    CCAAACAATT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327000.1
    CDS400..3894
    Translation

    Target ORF information:

    RefSeq Version XM_015471514.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCAGA GGAGGACAAG AGGGAGGACC AGAGATGCAG AGGCGTCCCT GAAAGCCTCC 
    ATCCTCGCCC GGGACTGCGC CACGGCCGCC GCCATCGTGT TCCTGAGCGA CCGTCTCCTG
    TACGGGCTCG ACGTCTCGGG CCAGCTGCTG CAGGTCGCCA AAGCGCTGCA CAGGCTGCAG
    CCCGCGACGC CCATCGCCCC GCAGGTGGTC ATCCGCCAGG CCCGAATCTC GATGCATGCA
    GGGAAACTTT TAAAAGCAGA GTATATCCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TATACAGAAA TGAAAGCGAC AAGGTCCTGG TGCAGTCAGT CTGCATACAG
    ATAAGAGGGC AAATTCTGCA GAAGTTGGGG ATGTGGTATG AAGCAGCTGA GTTAATATGG
    GCCTCAATCA TGGGATACCT GACACTTCCT CAGCCAGACA AAAAGGGCAT TTCCACATCA
    CTAGGTATAT TGGCAGACAT CTTCGTCTCC ATGAGCAAGA AAGATTATGA AAAGTTTAAA
    AGCAATCCTG ACATTAACCT GGGCTTGCTG AAGGAGTTTG ACCATCACTT GCTGTCAGCC
    GCTGAGGCCT GCAAGCTGGC GGCTGCCTTC AGCCCCTACA CGCCCCTGTT CGTGCTCACG
    GCTATGAATA TCCGTGGCAC GTGCCTCTTG TCATATAGTA GTTCTGAGGA CTGTCCTCCA
    GGAATGAAAA ATTCATATTT ATATGAAGCC AAAGAAGCCT TTGAAATTGG CCTCCTCACC
    AAGAAACGTG ATGAACCAGT TACTGGAAGA CAGGAGCTCC ATAGTTTTCT TAAAGCTGCT
    TTTGGTCTCA CCACTGTGCA CCAAAGACTC TATGGGGAGA TGGAGACCGT CCGAACAGCA
    AGTCAGCTCT GTAGTGAAGC TATGGGAAAG CTGTATACTT TCAGCAATTC CTCCAGAAGT
    CAGGAGAGAG AAGCTGTCTC TCAGGAAATC ATGTCTGTTA TCACCCGGGT GAAGGAACAG
    TTACAAGTTC AAAGCTTCTC AAATTTAGAT GACAAGTCTT ACGTTCCAGA GGGATTCAAG
    CGTGGGTTAG AGAAGCCCAT CTTACATGGG CAAGTGGATT TCCCAAAAAT CCTTGAAGCC
    TATTCACAGC ACCACACTTC AGTTTGTGAA CTATTTGAAA GCACTTGTGG GAACAACAAA
    AATAAACAGA AAGGTATGAA AACAGGAGTC TGTATCACAG ATCTAAAGAC AGAAACACAA
    AACACAGATA CCGTGAGTGC CACGGAGGAC AAAGCACATT TTGAAAAAGG CAGGGTGATA
    TCTTCTTCCC GAATGGCGAT GAACAGTCAG GAGAAACTCA GGAGGGCGGG AAGGAGAAAC
    TGGGCCGGTT CTGATGCGTT TCGAGTCTCC GTGGATGAAG ATGTGGAGAC CGAAGCTGAG
    TCAACAGACC ACAGCAATGG TGGGGGAGCT GTCCTGAACA AGTCTCTGAG TGACAGCCTG
    AGCTCCAGTT CTTGGAGCCA GTTATCAGGG TACAAATCTT CTACAAGCTG GGAGGAAGTG
    AATTATCATG TTGATGATAT GTCTGCCGGA AGAGAGCTGA GCAAGGAGGG GCATCTTGTG
    GACACGCAGT GTTCCACTGC TCTGTCAGAC GAGCAGGAGG TGAATGGGGT GAGCAGAGCT
    GAGCACCCAC TGTCTTCAGA GCTGCATGGT CTCTCTCTCC AGGTGCCCAG GGATGACAGG
    TTAGAGTCTT CTCAATGTCA GCTGCACAAG TCCACGCCCT TGGCAACCTT CCCACCTCAC
    AGCACAACAG GCACTTCATT GGCCTCTGGT GGAGGGTGGT TTAAAGAAGG CATGCAGGAA
    GTTGAAAATG TGGGGTCCAG AAATACTTCT GCTCATTCCA GACCCTTATT TTGTTCTGCT
    TCTTGGACTT CTGATTCTGG TTGGCCTAAG AACATGACCA CGTATCCTTC CATCCAAGAA
    GAAGAAACCT TTGAAACAAT TGGTGAGTTT CAAGAAATCA ACGGAGATGC CAAGGATGGG
    GATGAAGAAG AGAAGAGAGA AGAAATCAAA GGAGGCACAG GTCCTCCATT TAAAGATAGC
    CCCTGCTGGG TTGACCCAGA AGGAAAAATA GCAGAAAGAG CAGAAGATGT TCCCGTAGGC
    TTCCACTCAG TCGTGGACGA GTCTCGGGAC AAAAGCTGCA TGGTAGCATG TAACTCCTAC
    ACTCCTCATT GGCTTGTTGA AAACCCTAAC TCGGGAAAAA ATGGTCTCTC AGTCAAAGAG
    CAGGGCATTG ACCCTGATGC CTCCACGGTG GATGAGGAGA GCCCACTGCT GGGCAGCGCA
    GATGTTCACA CCACGAGCAC ACGTGGCTCC CCGAGACCGT GCACTTGGAA GCAGCTGCAC
    GTTCAGGGAG CCCAGATCCC CAGTTCCTCA GTAAGCAGAA AGACTTCCAC CCCTGTCCTC
    AGCGAGGACT GCACTACCAC GGAGGAAGCA AATGAACCTG GAAACCTGCT AAACTGCAGC
    CAGAACTCCA GCTTATCCTC ATCTTGGTGG CTGAAATCAC GGGCAATCTC CAGTGGTTCT
    TCTGAGGGAG AAAATCCATG GTCTTTTCTG AATTCCAGTG GAAGTTCTTT TGTTTCATTG
    CCAGGAATGA CAAAGCAGGC GATCCTTGAG GCCCGCACCC TGCAGCCTGA TGACTTTGAA
    AAACTCTTGG CAGGGGTGAG GCATGATTGG TTGTTCCAGA GACTGGAGCA TACAGGCCTT
    TTTAAATCCA GACAACTCCA CGGAGCACAT AGTGCTCTTT TGTTAAAATA TTCCAAGAAA
    TCTGAACTGT GGACAGCCCA AGAAACTGTT GTATATTTGG GGGACTACCT ACATGTGACA
    AAGAAAGGCA GACAAAGAAA TGCATTTTGG GTACATCATC TTCATCAAGA AGAAACTCTG
    GGCAGATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC TCTGGCACCA CTTCACTGAT
    GTGGAGAGGC AGATGACCGC ACAGCACTAC GTGACAGAAT TTAACAAGAG ACTTTACGAG
    CAGAAGATTC ACACGCAGAT ATTCTACATA CCGTCCTCAG TATTGCTGAT TTTAGAAGGC
    AAGACAATAA AAGGATGTGT CAGTGTGGAA CCTTACATGC TAGGGGAATT TGTAAAATTA
    TCAAATAACA CAAAAGTGGT GAAAACAGAA TACAAAGCCA CAGAATACGG CTTGGCGTAT
    GGCCACTTTT CTTATGAATT TTCCAATCAT AGAGATGTTG TGGTTGATTT ACAAGGTTGG
    GTGACTGGGA ATGGAAAAGG GCTCATCTAC CTCACAGATC CCCAGATTCA CTCTGTTGAT
    CAGAAAGATG TCACTACCAA TTTTGGAAAG AAAGGAATTT TCTACTTCTT TAATAACCAG
    CATGTGGAAT GTAATGAAAT ATGCCATCGT CTTTCTTTGA CAAGACCTTC GATTGAGAAA
    CCAAACAATT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20203
    Clone ID Related Accession (Same CDS sequence) XM_015471514.1 , XM_015463700.1
    Accession Version XM_015463700.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCAGA GGAGGACAAG AGGGAGGACC AGAGATGCAG AGGCGTCCCT GAAAGCCTCC 
    ATCCTCGCCC GGGACTGCGC CACGGCCGCC GCCATCGTGT TCCTGAGCGA CCGTCTCCTG
    TACGGGCTCG ACGTCTCGGG CCAGCTGCTG CAGGTCGCCA AAGCGCTGCA CAGGCTGCAG
    CCCGCGACGC CCATCGCCCC GCAGGTGGTC ATCCGCCAGG CCCGAATCTC GATGCATGCA
    GGGAAACTTT TAAAAGCAGA GTATATCCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TATACAGAAA TGAAAGCGAC AAGGTCCTGG TGCAGTCAGT CTGCATACAG
    ATAAGAGGGC AAATTCTGCA GAAGTTGGGG ATGTGGTATG AAGCAGCTGA GTTAATATGG
    GCCTCAATCA TGGGATACCT GACACTTCCT CAGCCAGACA AAAAGGGCAT TTCCACATCA
    CTAGGTATAT TGGCAGACAT CTTCGTCTCC ATGAGCAAGA AAGATTATGA AAAGTTTAAA
    AGCAATCCTG ACATTAACCT GGGCTTGCTG AAGGAGTTTG ACCATCACTT GCTGTCAGCC
    GCTGAGGCCT GCAAGCTGGC GGCTGCCTTC AGCCCCTACA CGCCCCTGTT CGTGCTCACG
    GCTATGAATA TCCGTGGCAC GTGCCTCTTG TCATATAGTA GTTCTGAGGA CTGTCCTCCA
    GGAATGAAAA ATTCATATTT ATATGAAGCC AAAGAAGCCT TTGAAATTGG CCTCCTCACC
    AAGAAACGTG ATGAACCAGT TACTGGAAGA CAGGAGCTCC ATAGTTTTCT TAAAGCTGCT
    TTTGGTCTCA CCACTGTGCA CCAAAGACTC TATGGGGAGA TGGAGACCGT CCGAACAGCA
    AGTCAGCTCT GTAGTGAAGC TATGGGAAAG CTGTATACTT TCAGCAATTC CTCCAGAAGT
    CAGGAGAGAG AAGCTGTCTC TCAGGAAATC ATGTCTGTTA TCACCCGGGT GAAGGAACAG
    TTACAAGTTC AAAGCTTCTC AAATTTAGAT GACAAGTCTT ACGTTCCAGA GGGATTCAAG
    CGTGGGTTAG AGAAGCCCAT CTTACATGGG CAAGTGGATT TCCCAAAAAT CCTTGAAGCC
    TATTCACAGC ACCACACTTC AGTTTGTGAA CTATTTGAAA GCACTTGTGG GAACAACAAA
    AATAAACAGA AAGGTATGAA AACAGGAGTC TGTATCACAG ATCTAAAGAC AGAAACACAA
    AACACAGATA CCGTGAGTGC CACGGAGGAC AAAGCACATT TTGAAAAAGG CAGGGTGATA
    TCTTCTTCCC GAATGGCGAT GAACAGTCAG GAGAAACTCA GGAGGGCGGG AAGGAGAAAC
    TGGGCCGGTT CTGATGCGTT TCGAGTCTCC GTGGATGAAG ATGTGGAGAC CGAAGCTGAG
    TCAACAGACC ACAGCAATGG TGGGGGAGCT GTCCTGAACA AGTCTCTGAG TGACAGCCTG
    AGCTCCAGTT CTTGGAGCCA GTTATCAGGG TACAAATCTT CTACAAGCTG GGAGGAAGTG
    AATTATCATG TTGATGATAT GTCTGCCGGA AGAGAGCTGA GCAAGGAGGG GCATCTTGTG
    GACACGCAGT GTTCCACTGC TCTGTCAGAC GAGCAGGAGG TGAATGGGGT GAGCAGAGCT
    GAGCACCCAC TGTCTTCAGA GCTGCATGGT CTCTCTCTCC AGGTGCCCAG GGATGACAGG
    TTAGAGTCTT CTCAATGTCA GCTGCACAAG TCCACGCCCT TGGCAACCTT CCCACCTCAC
    AGCACAACAG GCACTTCATT GGCCTCTGGT GGAGGGTGGT TTAAAGAAGG CATGCAGGAA
    GTTGAAAATG TGGGGTCCAG AAATACTTCT GCTCATTCCA GACCCTTATT TTGTTCTGCT
    TCTTGGACTT CTGATTCTGG TTGGCCTAAG AACATGACCA CGTATCCTTC CATCCAAGAA
    GAAGAAACCT TTGAAACAAT TGGTGAGTTT CAAGAAATCA ACGGAGATGC CAAGGATGGG
    GATGAAGAAG AGAAGAGAGA AGAAATCAAA GGAGGCACAG GTCCTCCATT TAAAGATAGC
    CCCTGCTGGG TTGACCCAGA AGGAAAAATA GCAGAAAGAG CAGAAGATGT TCCCGTAGGC
    TTCCACTCAG TCGTGGACGA GTCTCGGGAC AAAAGCTGCA TGGTAGCATG TAACTCCTAC
    ACTCCTCATT GGCTTGTTGA AAACCCTAAC TCGGGAAAAA ATGGTCTCTC AGTCAAAGAG
    CAGGGCATTG ACCCTGATGC CTCCACGGTG GATGAGGAGA GCCCACTGCT GGGCAGCGCA
    GATGTTCACA CCACGAGCAC ACGTGGCTCC CCGAGACCGT GCACTTGGAA GCAGCTGCAC
    GTTCAGGGAG CCCAGATCCC CAGTTCCTCA GTAAGCAGAA AGACTTCCAC CCCTGTCCTC
    AGCGAGGACT GCACTACCAC GGAGGAAGCA AATGAACCTG GAAACCTGCT AAACTGCAGC
    CAGAACTCCA GCTTATCCTC ATCTTGGTGG CTGAAATCAC GGGCAATCTC CAGTGGTTCT
    TCTGAGGGAG AAAATCCATG GTCTTTTCTG AATTCCAGTG GAAGTTCTTT TGTTTCATTG
    CCAGGAATGA CAAAGCAGGC GATCCTTGAG GCCCGCACCC TGCAGCCTGA TGACTTTGAA
    AAACTCTTGG CAGGGGTGAG GCATGATTGG TTGTTCCAGA GACTGGAGCA TACAGGCCTT
    TTTAAATCCA GACAACTCCA CGGAGCACAT AGTGCTCTTT TGTTAAAATA TTCCAAGAAA
    TCTGAACTGT GGACAGCCCA AGAAACTGTT GTATATTTGG GGGACTACCT ACATGTGACA
    AAGAAAGGCA GACAAAGAAA TGCATTTTGG GTACATCATC TTCATCAAGA AGAAACTCTG
    GGCAGATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC TCTGGCACCA CTTCACTGAT
    GTGGAGAGGC AGATGACCGC ACAGCACTAC GTGACAGAAT TTAACAAGAG ACTTTACGAG
    CAGAAGATTC ACACGCAGAT ATTCTACATA CCGTCCTCAG TATTGCTGAT TTTAGAAGGC
    AAGACAATAA AAGGATGTGT CAGTGTGGAA CCTTACATGC TAGGGGAATT TGTAAAATTA
    TCAAATAACA CAAAAGTGGT GAAAACAGAA TACAAAGCCA CAGAATACGG CTTGGCGTAT
    GGCCACTTTT CTTATGAATT TTCCAATCAT AGAGATGTTG TGGTTGATTT ACAAGGTTGG
    GTGACTGGGA ATGGAAAAGG GCTCATCTAC CTCACAGATC CCCAGATTCA CTCTGTTGAT
    CAGAAAGATG TCACTACCAA TTTTGGAAAG AAAGGAATTT TCTACTTCTT TAATAACCAG
    CATGTGGAAT GTAATGAAAT ATGCCATCGT CTTTCTTTGA CAAGACCTTC GATTGAGAAA
    CCAAACAATT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319186.1
    CDS400..3894
    Translation

    Target ORF information:

    RefSeq Version XM_015463700.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463700.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCAGA GGAGGACAAG AGGGAGGACC AGAGATGCAG AGGCGTCCCT GAAAGCCTCC 
    ATCCTCGCCC GGGACTGCGC CACGGCCGCC GCCATCGTGT TCCTGAGCGA CCGTCTCCTG
    TACGGGCTCG ACGTCTCGGG CCAGCTGCTG CAGGTCGCCA AAGCGCTGCA CAGGCTGCAG
    CCCGCGACGC CCATCGCCCC GCAGGTGGTC ATCCGCCAGG CCCGAATCTC GATGCATGCA
    GGGAAACTTT TAAAAGCAGA GTATATCCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TATACAGAAA TGAAAGCGAC AAGGTCCTGG TGCAGTCAGT CTGCATACAG
    ATAAGAGGGC AAATTCTGCA GAAGTTGGGG ATGTGGTATG AAGCAGCTGA GTTAATATGG
    GCCTCAATCA TGGGATACCT GACACTTCCT CAGCCAGACA AAAAGGGCAT TTCCACATCA
    CTAGGTATAT TGGCAGACAT CTTCGTCTCC ATGAGCAAGA AAGATTATGA AAAGTTTAAA
    AGCAATCCTG ACATTAACCT GGGCTTGCTG AAGGAGTTTG ACCATCACTT GCTGTCAGCC
    GCTGAGGCCT GCAAGCTGGC GGCTGCCTTC AGCCCCTACA CGCCCCTGTT CGTGCTCACG
    GCTATGAATA TCCGTGGCAC GTGCCTCTTG TCATATAGTA GTTCTGAGGA CTGTCCTCCA
    GGAATGAAAA ATTCATATTT ATATGAAGCC AAAGAAGCCT TTGAAATTGG CCTCCTCACC
    AAGAAACGTG ATGAACCAGT TACTGGAAGA CAGGAGCTCC ATAGTTTTCT TAAAGCTGCT
    TTTGGTCTCA CCACTGTGCA CCAAAGACTC TATGGGGAGA TGGAGACCGT CCGAACAGCA
    AGTCAGCTCT GTAGTGAAGC TATGGGAAAG CTGTATACTT TCAGCAATTC CTCCAGAAGT
    CAGGAGAGAG AAGCTGTCTC TCAGGAAATC ATGTCTGTTA TCACCCGGGT GAAGGAACAG
    TTACAAGTTC AAAGCTTCTC AAATTTAGAT GACAAGTCTT ACGTTCCAGA GGGATTCAAG
    CGTGGGTTAG AGAAGCCCAT CTTACATGGG CAAGTGGATT TCCCAAAAAT CCTTGAAGCC
    TATTCACAGC ACCACACTTC AGTTTGTGAA CTATTTGAAA GCACTTGTGG GAACAACAAA
    AATAAACAGA AAGGTATGAA AACAGGAGTC TGTATCACAG ATCTAAAGAC AGAAACACAA
    AACACAGATA CCGTGAGTGC CACGGAGGAC AAAGCACATT TTGAAAAAGG CAGGGTGATA
    TCTTCTTCCC GAATGGCGAT GAACAGTCAG GAGAAACTCA GGAGGGCGGG AAGGAGAAAC
    TGGGCCGGTT CTGATGCGTT TCGAGTCTCC GTGGATGAAG ATGTGGAGAC CGAAGCTGAG
    TCAACAGACC ACAGCAATGG TGGGGGAGCT GTCCTGAACA AGTCTCTGAG TGACAGCCTG
    AGCTCCAGTT CTTGGAGCCA GTTATCAGGG TACAAATCTT CTACAAGCTG GGAGGAAGTG
    AATTATCATG TTGATGATAT GTCTGCCGGA AGAGAGCTGA GCAAGGAGGG GCATCTTGTG
    GACACGCAGT GTTCCACTGC TCTGTCAGAC GAGCAGGAGG TGAATGGGGT GAGCAGAGCT
    GAGCACCCAC TGTCTTCAGA GCTGCATGGT CTCTCTCTCC AGGTGCCCAG GGATGACAGG
    TTAGAGTCTT CTCAATGTCA GCTGCACAAG TCCACGCCCT TGGCAACCTT CCCACCTCAC
    AGCACAACAG GCACTTCATT GGCCTCTGGT GGAGGGTGGT TTAAAGAAGG CATGCAGGAA
    GTTGAAAATG TGGGGTCCAG AAATACTTCT GCTCATTCCA GACCCTTATT TTGTTCTGCT
    TCTTGGACTT CTGATTCTGG TTGGCCTAAG AACATGACCA CGTATCCTTC CATCCAAGAA
    GAAGAAACCT TTGAAACAAT TGGTGAGTTT CAAGAAATCA ACGGAGATGC CAAGGATGGG
    GATGAAGAAG AGAAGAGAGA AGAAATCAAA GGAGGCACAG GTCCTCCATT TAAAGATAGC
    CCCTGCTGGG TTGACCCAGA AGGAAAAATA GCAGAAAGAG CAGAAGATGT TCCCGTAGGC
    TTCCACTCAG TCGTGGACGA GTCTCGGGAC AAAAGCTGCA TGGTAGCATG TAACTCCTAC
    ACTCCTCATT GGCTTGTTGA AAACCCTAAC TCGGGAAAAA ATGGTCTCTC AGTCAAAGAG
    CAGGGCATTG ACCCTGATGC CTCCACGGTG GATGAGGAGA GCCCACTGCT GGGCAGCGCA
    GATGTTCACA CCACGAGCAC ACGTGGCTCC CCGAGACCGT GCACTTGGAA GCAGCTGCAC
    GTTCAGGGAG CCCAGATCCC CAGTTCCTCA GTAAGCAGAA AGACTTCCAC CCCTGTCCTC
    AGCGAGGACT GCACTACCAC GGAGGAAGCA AATGAACCTG GAAACCTGCT AAACTGCAGC
    CAGAACTCCA GCTTATCCTC ATCTTGGTGG CTGAAATCAC GGGCAATCTC CAGTGGTTCT
    TCTGAGGGAG AAAATCCATG GTCTTTTCTG AATTCCAGTG GAAGTTCTTT TGTTTCATTG
    CCAGGAATGA CAAAGCAGGC GATCCTTGAG GCCCGCACCC TGCAGCCTGA TGACTTTGAA
    AAACTCTTGG CAGGGGTGAG GCATGATTGG TTGTTCCAGA GACTGGAGCA TACAGGCCTT
    TTTAAATCCA GACAACTCCA CGGAGCACAT AGTGCTCTTT TGTTAAAATA TTCCAAGAAA
    TCTGAACTGT GGACAGCCCA AGAAACTGTT GTATATTTGG GGGACTACCT ACATGTGACA
    AAGAAAGGCA GACAAAGAAA TGCATTTTGG GTACATCATC TTCATCAAGA AGAAACTCTG
    GGCAGATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC TCTGGCACCA CTTCACTGAT
    GTGGAGAGGC AGATGACCGC ACAGCACTAC GTGACAGAAT TTAACAAGAG ACTTTACGAG
    CAGAAGATTC ACACGCAGAT ATTCTACATA CCGTCCTCAG TATTGCTGAT TTTAGAAGGC
    AAGACAATAA AAGGATGTGT CAGTGTGGAA CCTTACATGC TAGGGGAATT TGTAAAATTA
    TCAAATAACA CAAAAGTGGT GAAAACAGAA TACAAAGCCA CAGAATACGG CTTGGCGTAT
    GGCCACTTTT CTTATGAATT TTCCAATCAT AGAGATGTTG TGGTTGATTT ACAAGGTTGG
    GTGACTGGGA ATGGAAAAGG GCTCATCTAC CTCACAGATC CCCAGATTCA CTCTGTTGAT
    CAGAAAGATG TCACTACCAA TTTTGGAAAG AAAGGAATTT TCTACTTCTT TAATAACCAG
    CATGTGGAAT GTAATGAAAT ATGCCATCGT CTTTCTTTGA CAAGACCTTC GATTGAGAAA
    CCAAACAATT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20205
    Clone ID Related Accession (Same CDS sequence) XM_015463702.1 , XM_015471516.1
    Accession Version XM_015463702.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC
    TCTGGCACCA CTTCACTGAT GTGGAGAGGC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319188.1
    CDS325..3420
    Translation

    Target ORF information:

    RefSeq Version XM_015463702.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463702.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC
    TCTGGCACCA CTTCACTGAT GTGGAGAGGC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20205
    Clone ID Related Accession (Same CDS sequence) XM_015463702.1 , XM_015471516.1
    Accession Version XM_015471516.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC
    TCTGGCACCA CTTCACTGAT GTGGAGAGGC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327002.1
    CDS325..3420
    Translation

    Target ORF information:

    RefSeq Version XM_015471516.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471516.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGGAAA
    CTTTTAAAAG CAGAGTATAT CCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAATATG TTGGGAAAGA ATATAAGGAG CAAAAGGGGC
    TCTGGCACCA CTTCACTGAT GTGGAGAGGC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50880
    Clone ID Related Accession (Same CDS sequence) XM_015463698.1 , XM_015471512.1 , XM_015471512.2
    Accession Version XM_015463698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319184.1
    CDS322..3990
    Translation

    Target ORF information:

    RefSeq Version XM_015463698.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50880
    Clone ID Related Accession (Same CDS sequence) XM_015463698.1 , XM_015471512.1 , XM_015471512.2
    Accession Version XM_015471512.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326998.1
    CDS322..3990
    Translation

    Target ORF information:

    RefSeq Version XM_015471512.1
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa50880
    Clone ID Related Accession (Same CDS sequence) XM_015463698.1 , XM_015471512.1 , XM_015471512.2
    Accession Version XM_015471512.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471512.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326998.1
    CDS290..3958
    Translation

    Target ORF information:

    RefSeq Version XM_015471512.2
    Organism Bos taurus(cattle)
    Definition Bos taurus alpha kinase 1 (ALPK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471512.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGCTGT AGCTACGCTG CTGCAAGAGT GCAAGCAAGT GCTGGACCAG 
    CTCCTGTTGG AGGCGTCAGA TGTGTCAGAG GAGGACAAGA GGGAGGACCA GAGATGCAGA
    GCCTCACTCC CCAGCGAGTT AAGAACCTTG ATCCAGGAGG CAAAGGAAAT GAAATGGCCC
    TTCGTGCCTG AAAAGTGGCA ATACAAACAA GCTGTAGGCC CAGAGGACAA AACAAACCTG
    CAGGATGTGA TTGGCGCCAG CTTGCAACAG TTACTGGCGT CCCTGAAAGC CTCCATCCTC
    GCCCGGGACT GCGCCACGGC CGCCGCCATC GTGTTCCTGA GCGACCGTCT CCTGTACGGG
    CTCGACGTCT CGGGCCAGCT GCTGCAGGTC GCCAAAGCGC TGCACAGGCT GCAGCCCGCG
    ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAGGCCCGAA TCTCGATGCA TGCAGGTACC
    TGGCTATACA GAAATGAAAG CGACAAGGTC CTGGTGCAGT CAGTCTGCAT ACAGATAAGA
    GGGCAAATTC TGCAGAAGTT GGGGATGTGG TATGAAGCAG CTGAGTTAAT ATGGGCCTCA
    ATCATGGGAT ACCTGACACT TCCTCAGCCA GACAAAAAGG GCATTTCCAC ATCACTAGGT
    ATATTGGCAG ACATCTTCGT CTCCATGAGC AAGAAAGATT ATGAAAAGTT TAAAAGCAAT
    CCTGACATTA ACCTGGGCTT GCTGAAGGAG TTTGACCATC ACTTGCTGTC AGCCGCTGAG
    GCCTGCAAGC TGGCGGCTGC CTTCAGCCCC TACACGCCCC TGTTCGTGCT CACGGCTATG
    AATATCCGTG GCACGTGCCT CTTGTCATAT AGTAGTTCTG AGGACTGTCC TCCAGGAATG
    AAAAATTCAT ATTTATATGA AGCCAAAGAA GCCTTTGAAA TTGGCCTCCT CACCAAGAAA
    CGTGATGAAC CAGTTACTGG AAGACAGGAG CTCCATAGTT TTCTTAAAGC TGCTTTTGGT
    CTCACCACTG TGCACCAAAG ACTCTATGGG GAGATGGAGA CCGTCCGAAC AGCAAGTCAG
    CTCTGTAGTG AAGCTATGGG AAAGCTGTAT ACTTTCAGCA ATTCCTCCAG AAGTCAGGAG
    AGAGAAGCTG TCTCTCAGGA AATCATGTCT GTTATCACCC GGGTGAAGGA ACAGTTACAA
    GTTCAAAGCT TCTCAAATTT AGATGACAAG TCTTACGTTC CAGAGGGATT CAAGCGTGGG
    TTAGAGAAGC CCATCTTACA TGGGCAAGTG GATTTCCCAA AAATCCTTGA AGCCTATTCA
    CAGCACCACA CTTCAGTTTG TGAACTATTT GAAAGCACTT GTGGGAACAA CAAAAATAAA
    CAGAAAGGTA TGAAAACAGG AGTCTGTATC ACAGATCTAA AGACAGAAAC ACAAAACACA
    GATACCGTGA GTGCCACGGA GGACAAAGCA CATTTTGAAA AAGGCAGGGT GATATCTTCT
    TCCCGAATGG CGATGAACAG TCAGGAGAAA CTCAGGAGGG CGGGAAGGAG AAACTGGGCC
    GGTTCTGATG CGTTTCGAGT CTCCGTGGAT GAAGATGTGG AGACCGAAGC TGAGTCAACA
    GACCACAGCA ATGGTGGGGG AGCTGTCCTG AACAAGTCTC TGAGTGACAG CCTGAGCTCC
    AGTTCTTGGA GCCAGTTATC AGGGTACAAA TCTTCTACAA GCTGGGAGGA AGTGAATTAT
    CATGTTGATG ATATGTCTGC CGGAAGAGAG CTGAGCAAGG AGGGGCATCT TGTGGACACG
    CAGTGTTCCA CTGCTCTGTC AGACGAGCAG GAGGTGAATG GGGTGAGCAG AGCTGAGCAC
    CCACTGTCTT CAGAGCTGCA TGGTCTCTCT CTCCAGGTGC CCAGGGATGA CAGGTTAGAG
    TCTTCTCAAT GTCAGCTGCA CAAGTCCACG CCCTTGGCAA CCTTCCCACC TCACAGCACA
    ACAGGCACTT CATTGGCCTC TGGTGGAGGG TGGTTTAAAG AAGGCATGCA GGAAGTTGAA
    AATGTGGGGT CCAGAAATAC TTCTGCTCAT TCCAGACCCT TATTTTGTTC TGCTTCTTGG
    ACTTCTGATT CTGGTTGGCC TAAGAACATG ACCACGTATC CTTCCATCCA AGAAGAAGAA
    ACCTTTGAAA CAATTGGTGA GTTTCAAGAA ATCAACGGAG ATGCCAAGGA TGGGGATGAA
    GAAGAGAAGA GAGAAGAAAT CAAAGGAGGC ACAGGTCCTC CATTTAAAGA TAGCCCCTGC
    TGGGTTGACC CAGAAGGAAA AATAGCAGAA AGAGCAGAAG ATGTTCCCGT AGGCTTCCAC
    TCAGTCGTGG ACGAGTCTCG GGACAAAAGC TGCATGGTAG CATGTAACTC CTACACTCCT
    CATTGGCTTG TTGAAAACCC TAACTCGGGA AAAAATGGTC TCTCAGTCAA AGAGCAGGGC
    ATTGACCCTG ATGCCTCCAC GGTGGATGAG GAGAGCCCAC TGCTGGGCAG CGCAGATGTT
    CACACCACGA GCACACGTGG CTCCCCGAGA CCGTGCACTT GGAAGCAGCT GCACGTTCAG
    GGAGCCCAGA TCCCCAGTTC CTCAGTAAGC AGAAAGACTT CCACCCCTGT CCTCAGCGAG
    GACTGCACTA CCACGGAGGA AGCAAATGAA CCTGGAAACC TGCTAAACTG CAGCCAGAAC
    TCCAGCTTAT CCTCATCTTG GTGGCTGAAA TCACGGGCAA TCTCCAGTGG TTCTTCTGAG
    GGAGAAAATC CATGGTCTTT TCTGAATTCC AGTGGAAGTT CTTTTGTTTC ATTGCCAGGA
    ATGACAAAGC AGGCGATCCT TGAGGCCCGC ACCCTGCAGC CTGATGACTT TGAAAAACTC
    TTGGCAGGGG TGAGGCATGA TTGGTTGTTC CAGAGACTGG AGCATACAGG CCTTTTTAAA
    TCCAGACAAC TCCACGGAGC ACATAGTGCT CTTTTGTTAA AATATTCCAA GAAATCTGAA
    CTGTGGACAG CCCAAGAAAC TGTTGTATAT TTGGGGGACT ACCTACATGT GACAAAGAAA
    GGCAGACAAA GAAATGCATT TTGGGTACAT CATCTTCATC AAGAAGAAAC TCTGGGCAGA
    TATGTTGGGA AAGAATATAA GGAGCAAAAG GGGCTCTGGC ACCACTTCAC TGATGTGGAG
    AGGCAGATGA CCGCACAGCA CTACGTGACA GAATTTAACA AGAGACTTTA CGAGCAGAAG
    ATTCACACGC AGATATTCTA CATACCGTCC TCAGTATTGC TGATTTTAGA AGGCAAGACA
    ATAAAAGGAT GTGTCAGTGT GGAACCTTAC ATGCTAGGGG AATTTGTAAA ATTATCAAAT
    AACACAAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT ACGGCTTGGC GTATGGCCAC
    TTTTCTTATG AATTTTCCAA TCATAGAGAT GTTGTGGTTG ATTTACAAGG TTGGGTGACT
    GGGAATGGAA AAGGGCTCAT CTACCTCACA GATCCCCAGA TTCACTCTGT TGATCAGAAA
    GATGTCACTA CCAATTTTGG AAAGAAAGGA ATTTTCTACT TCTTTAATAA CCAGCATGTG
    GAATGTAATG AAATATGCCA TCGTCTTTCT TTGACAAGAC CTTCGATTGA GAAACCAAAC
    AATTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.