RNF128 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF128
Entrez Gene ID	79589
Full Name	ring finger protein 128, E3 ubiquitin protein ligase
Synonyms	GRAIL
General protein information	Preferred Names ring finger protein 128, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase RNF128 RING-type E3 ubiquitin transferase RNF128 gene related to anergy in lymphocytes protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq22.3
Summary	The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].
Disorder MIM:	300439

mRNA and Protein(s)

mRNA	Protein	Name
NM_194463.2	NP_919445.1	E3 ubiquitin-protein ligase RNF128 isoform 1 precursor
NM_194463.1	NP_919445.1	E3 ubiquitin-protein ligase RNF128 isoform 1 precursor
NM_024539.3	NP_078815.3	E3 ubiquitin-protein ligase RNF128 isoform 2 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	rnf128a	NP_997780.1
Rattus norvegicus (Norway rat)	Rnf128	NP_001166820.1
Bos taurus (cattle)	RNF128	NP_001069539.1
Mus musculus (house mouse)	Rnf128	NP_075759.3
Gallus gallus (chicken)	RNF128	XP_420351.1
Homo sapiens (human)	RNF128	NP_919445.1
Canis lupus familiaris (dog)	RNF128	XP_549171.3

Gene Ontology
Bibliography

Related articles in PubMed

CD81 promotes both the degradation of transferrin receptor 2 (TfR2) and the Tfr2-mediated maintenance of hepcidin expression.
Chen J, Enns CA
The Journal of biological chemistry290(12)7841-50(2015 Mar)

Regulation of anergy-related ubiquitin E3 ligase, GRAIL, in murine models of colitis and patients with Crohn's disease.
Mukai A, Iijima H, Hiyama S, Fujii H, Shinzaki S, Inoue T, Shiraishi E, Kawai S, Araki M, Hayashi Y, Kondo J, Mizushima T, Kanto T, Egawa S, Nishida T, Tsujii M, Takehara T
Journal of gastroenterology49(12)1524-35(2014 Dec)

Grail as a molecular determinant for the functions of the tumor suppressor p53 in tumorigenesis.
Chen YC, Chan JY, Chiu YL, Liu ST, Lozano G, Wang SL, Ho CL, Huang SM
Cell death and differentiation20(5)732-43(2013 May)

The transmembrane E3 ligase GRAIL ubiquitinates and degrades CD83 on CD4 T cells.
Su LL, Iwai H, Lin JT, Fathman CG
Journal of immunology (Baltimore, Md. : 1950)183(1)438-44(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results show that expression of GRAIL is uniquely regulated by the specific miRNA in intestinal mucosa, and suggest that GRAIL may associate with the pathophysiology of Crohn's disease
Title: Regulation of anergy-related ubiquitin E3 ligase, GRAIL, in murine models of colitis and patients with Crohn's disease.

study shows that Grail has a novel, p53-dependent role in regulating the cell cycle and apoptosis
Title: Grail as a molecular determinant for the functions of the tumor suppressor p53 in tumorigenesis.

GRAIL can down-modulate the expression of CD83 antigen on CD4 T cells.
Title: The transmembrane E3 ligase GRAIL ubiquitinates and degrades CD83 on CD4 T cells.

endogenous GRAIL regulates general cell cycle and proliferation of primary naive CD4 T cells.
Title: Naive CD4 t cell proliferation is controlled by mammalian target of rapamycin regulation of GRAIL expression.

GRAIL captures and then ubiquitinates transmembrane proteins across the cell membrane
Title: The single subunit transmembrane E3 ligase gene related to anergy in lymphocytes (GRAIL) captures and then ubiquitinates transmembrane proteins across the cell membrane.

The following RNF128 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF128 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25413	NM_194463.2 Latest version!	Homo sapiens ring finger protein 128 (RNF128), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25622	NM_024539.3 Latest version!	Homo sapiens ring finger protein 128 (RNF128), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu25413	NM_194463.1	Homo sapiens ring finger protein 128, E3 ubiquitin protein ligase (RNF128), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF128 ( NM_194463.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_919445.1 Homo sapiens ring finger protein 128 (RNF128), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25413
Clone ID Related Accession (Same CDS sequence)	NM_194463.2 , NM_194463.1
Accession Version	NM_194463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF128 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK074264.1, BI600046.1 and BC012931.1. On Nov 23, 2018 this sequence version replaced NM_194463.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.179438.1, AK074264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000255499.3/ ENSP00000255499.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGCCGC CGCCTGGGGC CGGGGTCTCC TGCCGCGGTG GCTGCGGCTT TTCCAGATTG 
CTGGCATGGT GCTTCCTGCT GGCCCTGAGT CCGCAGGCAC CCGGTTCCCG GGGGGCTGAA 
GCAGTGTGGA CCGCGTACCT CAACGTGTCC TGGCGGGTTC CGCACACGGG AGTGAACCGT 
ACGGTGTGGG AGCTGAGCGA GGAGGGCGTG TACGGCCAGG ACTCGCCGCT GGAGCCTGTG 
GCTGGGGTCC TGGTACCGCC CGACGGGCCC GGGGCGCTTA ACGCCTGTAA CCCGCACACG 
AATTTCACGG TGCCCACGGT TTGGGGAAGC ACCGTGCAAG TCTCTTGGTT GGCCCTCATC 
CAACGCGGCG GGGGCTGCAC CTTCGCAGAC AAGATCCATC TGGCTTATGA GAGAGGGGCG 
TCTGGAGCCG TCATCTTTAA CTTCCCCGGG ACCCGCAATG AGGTCATCCC CATGTCTCAC 
CCGGGTGCAG TAGACATTGT TGCAATCATG ATCGGCAATC TGAAAGGCAC AAAAATTCTG 
CAATCTATTC AAAGAGGCAT ACAAGTGACA ATGGTCATAG AAGTAGGGAA AAAACATGGC 
CCTTGGGTGA ATCACTATTC AATTTTTTTC GTTTCTGTGT CCTTTTTTAT TATTACGGCG 
GCAACTGTGG GCTATTTTAT CTTTTATTCT GCTCGAAGGC TACGGAATGC AAGAGCTCAA 
AGCAGGAAGC AGAGGCAATT AAAGGCAGAT GCTAAAAAAG CTATTGGAAG GCTTCAACTA 
CGCACACTGA AACAAGGAGA CAAGGAAATT GGCCCTGATG GAGATAGTTG TGCTGTGTGC 
ATTGAATTGT ATAAACCAAA TGATTTGGTA CGCATCTTAA CGTGCAACCA TATTTTCCAT 
AAGACATGTG TTGACCCATG GCTGTTAGAA CACAGGACTT GCCCCATGTG CAAATGTGAC 
ATACTCAAAG CTTTGGGAAT TGAGGTGGAT GTTGAAGATG GATCAGTGTC TTTACAAGTC 
CCTGTATCCA ATGAAATATC TAATAGTGCC TCCTCCCATG AAGAGGATAA TCGCAGCGAG 
ACCGCATCAT CTGGATATGC TTCAGTACAG GGAACAGATG AACCGCCTCT GGAGGAACAC 
GTGCAGTCAA CAAATGAAAG TCTACAGCTG GTAAACCATG AAGCAAATTC TGTGGCAGTG 
GATGTTATTC CTCATGTTGA CAACCCAACC TTTGAAGAAG ACGAAACTCC TAATCAAGAG 
ACTGCTGTTC GAGAAATTAA ATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919445.1
CDS	214..1500
Translation	MGPPPGAGVSCRGGCGFSRLLAWCFLLALSPQAPGSRGAEAVWTAYLNVSWRVPHTGVNRTVWELSEEGVYGQDSPLEPVAGVLVPPDGPGALNACNPHTNFTVPTVWGSTVQVSWLALIQRGGGCTFADKIHLAYERGASGAVIFNFPGTRNEVIPMSHPGAVDIVAIMIGNLKGTKILQSIQRGIQVTMVIEVGKKHGPWVNHYSIFFVSVSFFIITAATVGYFIFYSARRLRNARAQSRKQRQLKADAKKAIGRLQLRTLKQGDKEIGPDGDSCAVCIELYKPNDLVRILTCNHIFHKTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDGSVSLQVPVSNEISNSASSHEEDNRSETASSGYASVQGTDEPPLEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQETAVREIKS

Target ORF information:

RefSeq Version	NM_194463.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 128 (RNF128), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194463.2

ORF Insert Sequence:

ATGGGGCCGC CGCCTGGGGC CGGGGTCTCC TGCCGCGGTG GCTGCGGCTT TTCCAGATTG 
CTGGCATGGT GCTTCCTGCT GGCCCTGAGT CCGCAGGCAC CCGGTTCCCG GGGGGCTGAA 
GCAGTGTGGA CCGCGTACCT CAACGTGTCC TGGCGGGTTC CGCACACGGG AGTGAACCGT 
ACGGTGTGGG AGCTGAGCGA GGAGGGCGTG TACGGCCAGG ACTCGCCGCT GGAGCCTGTG 
GCTGGGGTCC TGGTACCGCC CGACGGGCCC GGGGCGCTTA ACGCCTGTAA CCCGCACACG 
AATTTCACGG TGCCCACGGT TTGGGGAAGC ACCGTGCAAG TCTCTTGGTT GGCCCTCATC 
CAACGCGGCG GGGGCTGCAC CTTCGCAGAC AAGATCCATC TGGCTTATGA GAGAGGGGCG 
TCTGGAGCCG TCATCTTTAA CTTCCCCGGG ACCCGCAATG AGGTCATCCC CATGTCTCAC 
CCGGGTGCAG TAGACATTGT TGCAATCATG ATCGGCAATC TGAAAGGCAC AAAAATTCTG 
CAATCTATTC AAAGAGGCAT ACAAGTGACA ATGGTCATAG AAGTAGGGAA AAAACATGGC 
CCTTGGGTGA ATCACTATTC AATTTTTTTC GTTTCTGTGT CCTTTTTTAT TATTACGGCG 
GCAACTGTGG GCTATTTTAT CTTTTATTCT GCTCGAAGGC TACGGAATGC AAGAGCTCAA 
AGCAGGAAGC AGAGGCAATT AAAGGCAGAT GCTAAAAAAG CTATTGGAAG GCTTCAACTA 
CGCACACTGA AACAAGGAGA CAAGGAAATT GGCCCTGATG GAGATAGTTG TGCTGTGTGC 
ATTGAATTGT ATAAACCAAA TGATTTGGTA CGCATCTTAA CGTGCAACCA TATTTTCCAT 
AAGACATGTG TTGACCCATG GCTGTTAGAA CACAGGACTT GCCCCATGTG CAAATGTGAC 
ATACTCAAAG CTTTGGGAAT TGAGGTGGAT GTTGAAGATG GATCAGTGTC TTTACAAGTC 
CCTGTATCCA ATGAAATATC TAATAGTGCC TCCTCCCATG AAGAGGATAA TCGCAGCGAG 
ACCGCATCAT CTGGATATGC TTCAGTACAG GGAACAGATG AACCGCCTCT GGAGGAACAC 
GTGCAGTCAA CAAATGAAAG TCTACAGCTG GTAAACCATG AAGCAAATTC TGTGGCAGTG 
GATGTTATTC CTCATGTTGA CAACCCAACC TTTGAAGAAG ACGAAACTCC TAATCAAGAG 
ACTGCTGTTC GAGAAATTAA ATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF128 ( NM_194463.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_919445.1 Homo sapiens ring finger protein 128, E3 ubiquitin protein ligase (RNF128), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25413
Clone ID Related Accession (Same CDS sequence)	NM_194463.2 , NM_194463.1
Accession Version	NM_194463.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF128 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK074264.1, BI600046.1, BC012931.1 and AK027169.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK074264.1, SRR1660805.66680.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCCGC CGCCTGGGGC CGGGGTCTCC TGCCGCGGTG GCTGCGGCTT TTCCAGATTG 
CTGGCATGGT GCTTCCTGCT GGCCCTGAGT CCGCAGGCAC CCGGTTCCCG GGGGGCTGAA 
GCAGTGTGGA CCGCGTACCT CAACGTGTCC TGGCGGGTTC CGCACACGGG AGTGAACCGT 
ACGGTGTGGG AGCTGAGCGA GGAGGGCGTG TACGGCCAGG ACTCGCCGCT GGAGCCTGTG 
GCTGGGGTCC TGGTACCGCC CGACGGGCCC GGGGCGCTTA ACGCCTGTAA CCCGCACACG 
AATTTCACGG TGCCCACGGT TTGGGGAAGC ACCGTGCAAG TCTCTTGGTT GGCCCTCATC 
CAACGCGGCG GGGGCTGCAC CTTCGCAGAC AAGATCCATC TGGCTTATGA GAGAGGGGCG 
TCTGGAGCCG TCATCTTTAA CTTCCCCGGG ACCCGCAATG AGGTCATCCC CATGTCTCAC 
CCGGGTGCAG TAGACATTGT TGCAATCATG ATCGGCAATC TGAAAGGCAC AAAAATTCTG 
CAATCTATTC AAAGAGGCAT ACAAGTGACA ATGGTCATAG AAGTAGGGAA AAAACATGGC 
CCTTGGGTGA ATCACTATTC AATTTTTTTC GTTTCTGTGT CCTTTTTTAT TATTACGGCG 
GCAACTGTGG GCTATTTTAT CTTTTATTCT GCTCGAAGGC TACGGAATGC AAGAGCTCAA 
AGCAGGAAGC AGAGGCAATT AAAGGCAGAT GCTAAAAAAG CTATTGGAAG GCTTCAACTA 
CGCACACTGA AACAAGGAGA CAAGGAAATT GGCCCTGATG GAGATAGTTG TGCTGTGTGC 
ATTGAATTGT ATAAACCAAA TGATTTGGTA CGCATCTTAA CGTGCAACCA TATTTTCCAT 
AAGACATGTG TTGACCCATG GCTGTTAGAA CACAGGACTT GCCCCATGTG CAAATGTGAC 
ATACTCAAAG CTTTGGGAAT TGAGGTGGAT GTTGAAGATG GATCAGTGTC TTTACAAGTC 
CCTGTATCCA ATGAAATATC TAATAGTGCC TCCTCCCATG AAGAGGATAA TCGCAGCGAG 
ACCGCATCAT CTGGATATGC TTCAGTACAG GGAACAGATG AACCGCCTCT GGAGGAACAC 
GTGCAGTCAA CAAATGAAAG TCTACAGCTG GTAAACCATG AAGCAAATTC TGTGGCAGTG 
GATGTTATTC CTCATGTTGA CAACCCAACC TTTGAAGAAG ACGAAACTCC TAATCAAGAG 
ACTGCTGTTC GAGAAATTAA ATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919445.1
CDS	251..1537
Translation	MGPPPGAGVSCRGGCGFSRLLAWCFLLALSPQAPGSRGAEAVWTAYLNVSWRVPHTGVNRTVWELSEEGVYGQDSPLEPVAGVLVPPDGPGALNACNPHTNFTVPTVWGSTVQVSWLALIQRGGGCTFADKIHLAYERGASGAVIFNFPGTRNEVIPMSHPGAVDIVAIMIGNLKGTKILQSIQRGIQVTMVIEVGKKHGPWVNHYSIFFVSVSFFIITAATVGYFIFYSARRLRNARAQSRKQRQLKADAKKAIGRLQLRTLKQGDKEIGPDGDSCAVCIELYKPNDLVRILTCNHIFHKTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDGSVSLQVPVSNEISNSASSHEEDNRSETASSGYASVQGTDEPPLEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQETAVREIKS

Target ORF information:

RefSeq Version	NM_194463.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 128, E3 ubiquitin protein ligase (RNF128), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194463.1

ORF Insert Sequence:

ATGGGGCCGC CGCCTGGGGC CGGGGTCTCC TGCCGCGGTG GCTGCGGCTT TTCCAGATTG 
CTGGCATGGT GCTTCCTGCT GGCCCTGAGT CCGCAGGCAC CCGGTTCCCG GGGGGCTGAA 
GCAGTGTGGA CCGCGTACCT CAACGTGTCC TGGCGGGTTC CGCACACGGG AGTGAACCGT 
ACGGTGTGGG AGCTGAGCGA GGAGGGCGTG TACGGCCAGG ACTCGCCGCT GGAGCCTGTG 
GCTGGGGTCC TGGTACCGCC CGACGGGCCC GGGGCGCTTA ACGCCTGTAA CCCGCACACG 
AATTTCACGG TGCCCACGGT TTGGGGAAGC ACCGTGCAAG TCTCTTGGTT GGCCCTCATC 
CAACGCGGCG GGGGCTGCAC CTTCGCAGAC AAGATCCATC TGGCTTATGA GAGAGGGGCG 
TCTGGAGCCG TCATCTTTAA CTTCCCCGGG ACCCGCAATG AGGTCATCCC CATGTCTCAC 
CCGGGTGCAG TAGACATTGT TGCAATCATG ATCGGCAATC TGAAAGGCAC AAAAATTCTG 
CAATCTATTC AAAGAGGCAT ACAAGTGACA ATGGTCATAG AAGTAGGGAA AAAACATGGC 
CCTTGGGTGA ATCACTATTC AATTTTTTTC GTTTCTGTGT CCTTTTTTAT TATTACGGCG 
GCAACTGTGG GCTATTTTAT CTTTTATTCT GCTCGAAGGC TACGGAATGC AAGAGCTCAA 
AGCAGGAAGC AGAGGCAATT AAAGGCAGAT GCTAAAAAAG CTATTGGAAG GCTTCAACTA 
CGCACACTGA AACAAGGAGA CAAGGAAATT GGCCCTGATG GAGATAGTTG TGCTGTGTGC 
ATTGAATTGT ATAAACCAAA TGATTTGGTA CGCATCTTAA CGTGCAACCA TATTTTCCAT 
AAGACATGTG TTGACCCATG GCTGTTAGAA CACAGGACTT GCCCCATGTG CAAATGTGAC 
ATACTCAAAG CTTTGGGAAT TGAGGTGGAT GTTGAAGATG GATCAGTGTC TTTACAAGTC 
CCTGTATCCA ATGAAATATC TAATAGTGCC TCCTCCCATG AAGAGGATAA TCGCAGCGAG 
ACCGCATCAT CTGGATATGC TTCAGTACAG GGAACAGATG AACCGCCTCT GGAGGAACAC 
GTGCAGTCAA CAAATGAAAG TCTACAGCTG GTAAACCATG AAGCAAATTC TGTGGCAGTG 
GATGTTATTC CTCATGTTGA CAACCCAACC TTTGAAGAAG ACGAAACTCC TAATCAAGAG 
ACTGCTGTTC GAGAAATTAA ATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF128 ( NM_024539.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_078815.3 Homo sapiens ring finger protein 128 (RNF128), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25622
Clone ID Related Accession (Same CDS sequence)	NM_024539.3
Accession Version	NM_024539.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF128 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC036901.1, BC012931.1 and AK027169.1. This sequence is a reference standard in the RefSeqGene project. On Oct 8, 2003 this sequence version replaced NM_024539.2. Transcript Variant: This variant (2) has an alternate 5' end exon compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036901.1, AK126553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCAGG AGAATAGGTC CAGTTTTTTT TGGCTCCTTG TAATTTTTAC CTTTTTACTT 
AAAATTACAG CATCTTTTTC AATGAGTGCC TATGTGACTG TGACTTATTA CAATGAAACC 
AGCAACTACA CTGCAATAGA GACATGTGAA TGTGGCGTTT ATGGATTAGC TTCACCAGTG 
GCTAATGCTA TGGGAGTGGT AGGCATCCCT AAGAACAATA ACTACCAAGC TTGTGACCAC 
AACACTGAGT TTAGTAATAC TAAGAAGCCC TGGATTGCGC TGATAGAAAG AGGTAATTGT 
ACATTTTCAG AAAAAATTCA AACAGCGGGC AGAAGAAATG CTGATGCTGT TGTGATTTAC 
AATGCTCCAG AGACTGGCAA TCAGACGATA CAGATGGCAA ATTTTGGTGC AGTAGACATT 
GTTGCAATCA TGATCGGCAA TCTGAAAGGC ACAAAAATTC TGCAATCTAT TCAAAGAGGC 
ATACAAGTGA CAATGGTCAT AGAAGTAGGG AAAAAACATG GCCCTTGGGT GAATCACTAT 
TCAATTTTTT TCGTTTCTGT GTCCTTTTTT ATTATTACGG CGGCAACTGT GGGCTATTTT 
ATCTTTTATT CTGCTCGAAG GCTACGGAAT GCAAGAGCTC AAAGCAGGAA GCAGAGGCAA 
TTAAAGGCAG ATGCTAAAAA AGCTATTGGA AGGCTTCAAC TACGCACACT GAAACAAGGA 
GACAAGGAAA TTGGCCCTGA TGGAGATAGT TGTGCTGTGT GCATTGAATT GTATAAACCA 
AATGATTTGG TACGCATCTT AACGTGCAAC CATATTTTCC ATAAGACATG TGTTGACCCA 
TGGCTGTTAG AACACAGGAC TTGCCCCATG TGCAAATGTG ACATACTCAA AGCTTTGGGA 
ATTGAGGTGG ATGTTGAAGA TGGATCAGTG TCTTTACAAG TCCCTGTATC CAATGAAATA 
TCTAATAGTG CCTCCTCCCA TGAAGAGGAT AATCGCAGCG AGACCGCATC ATCTGGATAT 
GCTTCAGTAC AGGGAACAGA TGAACCGCCT CTGGAGGAAC ACGTGCAGTC AACAAATGAA 
AGTCTACAGC TGGTAAACCA TGAAGCAAAT TCTGTGGCAG TGGATGTTAT TCCTCATGTT 
GACAACCCAA CCTTTGAAGA AGACGAAACT CCTAATCAAG AGACTGCTGT TCGAGAAATT 
AAATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_078815.3
CDS	166..1374
Translation	MNQENRSSFFWLLVIFTFLLKITASFSMSAYVTVTYYNETSNYTAIETCECGVYGLASPVANAMGVVGIPKNNNYQACDHNTEFSNTKKPWIALIERGNCTFSEKIQTAGRRNADAVVIYNAPETGNQTIQMANFGAVDIVAIMIGNLKGTKILQSIQRGIQVTMVIEVGKKHGPWVNHYSIFFVSVSFFIITAATVGYFIFYSARRLRNARAQSRKQRQLKADAKKAIGRLQLRTLKQGDKEIGPDGDSCAVCIELYKPNDLVRILTCNHIFHKTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDGSVSLQVPVSNEISNSASSHEEDNRSETASSGYASVQGTDEPPLEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQETAVREIKS

Target ORF information:

RefSeq Version	NM_024539.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 128 (RNF128), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024539.3

ORF Insert Sequence:

ATGAACCAGG AGAATAGGTC CAGTTTTTTT TGGCTCCTTG TAATTTTTAC CTTTTTACTT 
AAAATTACAG CATCTTTTTC AATGAGTGCC TATGTGACTG TGACTTATTA CAATGAAACC 
AGCAACTACA CTGCAATAGA GACATGTGAA TGTGGCGTTT ATGGATTAGC TTCACCAGTG 
GCTAATGCTA TGGGAGTGGT AGGCATCCCT AAGAACAATA ACTACCAAGC TTGTGACCAC 
AACACTGAGT TTAGTAATAC TAAGAAGCCC TGGATTGCGC TGATAGAAAG AGGTAATTGT 
ACATTTTCAG AAAAAATTCA AACAGCGGGC AGAAGAAATG CTGATGCTGT TGTGATTTAC 
AATGCTCCAG AGACTGGCAA TCAGACGATA CAGATGGCAA ATTTTGGTGC AGTAGACATT 
GTTGCAATCA TGATCGGCAA TCTGAAAGGC ACAAAAATTC TGCAATCTAT TCAAAGAGGC 
ATACAAGTGA CAATGGTCAT AGAAGTAGGG AAAAAACATG GCCCTTGGGT GAATCACTAT 
TCAATTTTTT TCGTTTCTGT GTCCTTTTTT ATTATTACGG CGGCAACTGT GGGCTATTTT 
ATCTTTTATT CTGCTCGAAG GCTACGGAAT GCAAGAGCTC AAAGCAGGAA GCAGAGGCAA 
TTAAAGGCAG ATGCTAAAAA AGCTATTGGA AGGCTTCAAC TACGCACACT GAAACAAGGA 
GACAAGGAAA TTGGCCCTGA TGGAGATAGT TGTGCTGTGT GCATTGAATT GTATAAACCA 
AATGATTTGG TACGCATCTT AACGTGCAAC CATATTTTCC ATAAGACATG TGTTGACCCA 
TGGCTGTTAG AACACAGGAC TTGCCCCATG TGCAAATGTG ACATACTCAA AGCTTTGGGA 
ATTGAGGTGG ATGTTGAAGA TGGATCAGTG TCTTTACAAG TCCCTGTATC CAATGAAATA 
TCTAATAGTG CCTCCTCCCA TGAAGAGGAT AATCGCAGCG AGACCGCATC ATCTGGATAT 
GCTTCAGTAC AGGGAACAGA TGAACCGCCT CTGGAGGAAC ACGTGCAGTC AACAAATGAA 
AGTCTACAGC TGGTAAACCA TGAAGCAAAT TCTGTGGCAG TGGATGTTAT TCCTCATGTT 
GACAACCCAA CCTTTGAAGA AGACGAAACT CCTAATCAAG AGACTGCTGT TCGAGAAATT 
AAATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CD81 promotes both the degradation of transferrin receptor 2 (TfR2) and the Tfr2-mediated maintenance of hepcidin expression.
The Journal of biological chemistry290(12)7841-50(2015 Mar)
Chen J,Enns CA

Regulation of anergy-related ubiquitin E3 ligase, GRAIL, in murine models of colitis and patients with Crohn's disease.
Journal of gastroenterology49(12)1524-35(2014 Dec)
Mukai A,Iijima H,Hiyama S,Fujii H,Shinzaki S,Inoue T,Shiraishi E,Kawai S,Araki M,Hayashi Y,Kondo J,Mizushima T,Kanto T,Egawa S,Nishida T,Tsujii M,Takehara T

Grail as a molecular determinant for the functions of the tumor suppressor p53 in tumorigenesis.
Cell death and differentiation20(5)732-43(2013 May)
Chen YC,Chan JY,Chiu YL,Liu ST,Lozano G,Wang SL,Ho CL,Huang SM

The transmembrane E3 ligase GRAIL ubiquitinates and degrades CD83 on CD4 T cells.
Journal of immunology (Baltimore, Md. : 1950)183(1)438-44(2009 Jul)
Su LL,Iwai H,Lin JT,Fathman CG

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RNF128 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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