PLEKHF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLEKHF1
Entrez Gene ID	79156
Full Name	pleckstrin homology and FYVE domain containing 1
Synonyms	APPD,LAPF,PHAFIN1,ZFYVE15
General protein information	Preferred Names pleckstrin homology and FYVE domain containing 1 Names pleckstrin homology domain-containing family F member 1 PH and FYVE domain-containing protein 1 PH domain-containing family F member 1 apoptosis-inducing protein D lysosome-associated apoptosis-inducing protein containing PH and FYVE domains phafin 1 pleckstrin homology domain containing, family F (with FYVE domain) member 1 zinc finger FYVE domain-containing protein 15
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q12
Disorder MIM:	615200

mRNA and Protein(s)

mRNA	Protein	Name
XM_011527309.2	XP_011525611.1	pleckstrin homology domain-containing family F member 1 isoform X1
XM_005259256.3	XP_005259313.1	pleckstrin homology domain-containing family F member 1 isoform X2
XM_011527309.3	XP_011525611.1	pleckstrin homology domain-containing family F member 1 isoform X1
XM_006723378.1	XP_006723441.1	pleckstrin homology domain-containing family F member 1 isoform X1
XM_011527309.1	XP_011525611.1	pleckstrin homology domain-containing family F member 1 isoform X1
NM_024310.4	NP_077286.3	pleckstrin homology domain-containing family F member 1
NM_024310.5	NP_077286.3	pleckstrin homology domain-containing family F member 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PLEKHF1	NP_077286.3
Pan troglodytes (chimpanzee)	PLEKHF1	XP_001149430.1
	PLEKHF1	XP_001084522.1
Rattus norvegicus (Norway rat)	Plekhf1	NP_001013166.1
Bos taurus (cattle)	PLEKHF1	NP_001075937.1
Mus musculus (house mouse)	Plekhf1	NP_077724.2
Xenopus tropicalis (tropical clawed frog)	plekhf1	NP_001016010.1
Gallus gallus (chicken)	PLEKHF1	XP_004944264.1
Canis lupus familiaris (dog)	PLEKHF1	XP_005616858.1

Gene Ontology
Bibliography

Related articles in PubMed

Lysosomal targeting of phafin1 mediated by Rab7 induces autophagosome formation.
Lin WJ, Yang CY, Li LL, Yi YH, Chen KW, Lin YC, Liu CC, Lin CH
Biochemical and biophysical research communications417(1)35-42(2012 Jan)

The lysosome-associated apoptosis-inducing protein containing the pleckstrin homology (PH) and FYVE domains (LAPF), representative of a novel family of PH and FYVE domain-containing proteins, induces caspase-independent apoptosis via the lysosomal-mitochondrial pathway.
Chen W, Li N, Chen T, Han Y, Li C, Wang Y, He W, Zhang L, Wan T, Cao X
The Journal of biological chemistry280(49)40985-95(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that in addition to its role in endosome transport, phafin1 is also involved in lysosomal targeting and autophagosome formation.
Title: Lysosomal targeting of phafin1 mediated by Rab7 induces autophagosome formation.

functional characterization of LAPF, a novel lysosome-associated apoptosis-inducing protein containing PH and FYVE domains; LAPF may launch caspase-independent apoptosis through the lysosomal-mitochondrial pathway
Title: The lysosome-associated apoptosis-inducing protein containing the pleckstrin homology (PH) and FYVE domains (LAPF), representative of a novel family of PH and FYVE domain-containing proteins, induces caspase-independent apoptosis via the lysosomal-mitochondrial pathway.

The following PLEKHF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31082	NM_024310.5 Latest version!	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu31082	XM_006723378.1 Latest version!	Homo sapiens pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu31082	XM_011527309.3 Latest version!	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu52485	XM_005259256.3 Latest version!	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu31082	XM_011527309.2	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu31082	XM_011527309.1	Homo sapiens pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu31082	NM_024310.4	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLEKHF1 ( NM_024310.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_077286.3 Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), mRNA.

Price & Availability↑

CloneID	OHu31082
Clone ID Related Accession (Same CDS sequence)	XM_011527309.2 , XM_011527309.3 , XM_006723378.1 , XM_011527309.1 , NM_024310.4 , NM_024310.5
Accession Version	NM_024310.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	pleckstrin homology domain-containing family F member 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY037145.2 and BC018876.2. On Jul 25, 2007 this sequence version replaced NM_024310.3. ##Evidence-Data-START## Transcript exon combination :: BC018876.2, SRR1163658.182596.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077286.3
CDS	103..942
Translation	MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

Target ORF information:

RefSeq Version	NM_024310.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024310.4

ORF Insert Sequence:

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHF1 ( NM_024310.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_077286.3 Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), mRNA.

Price & Availability↑

CloneID	OHu31082
Clone ID Related Accession (Same CDS sequence)	XM_011527309.2 , XM_011527309.3 , XM_006723378.1 , XM_011527309.1 , NM_024310.4 , NM_024310.5
Accession Version	NM_024310.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	pleckstrin homology domain-containing family F member 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY037145.2 and BC018876.2. On Nov 22, 2018 this sequence version replaced NM_024310.4. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.593749.1, SRR3476690.974448.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000436066.4/ ENSP00000389787.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077286.3
CDS	62..901
Translation	MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

Target ORF information:

RefSeq Version	NM_024310.5
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024310.5

ORF Insert Sequence:

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHF1 ( XM_011527309.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525611.1 Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu31082
Clone ID Related Accession (Same CDS sequence)	XM_011527309.2 , XM_011527309.3 , XM_006723378.1 , XM_011527309.1 , NM_024310.4 , NM_024310.5
Accession Version	XM_011527309.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pleckstrin homology domain-containing family F member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525611.1
CDS	1377..2216
Translation	MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

Target ORF information:

RefSeq Version	XM_011527309.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527309.2

ORF Insert Sequence:

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHF1 ( XM_011527309.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525611.1 Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu31082
Clone ID Related Accession (Same CDS sequence)	XM_011527309.2 , XM_011527309.3 , XM_006723378.1 , XM_011527309.1 , NM_024310.4 , NM_024310.5
Accession Version	XM_011527309.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pleckstrin homology domain-containing family F member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011527309.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525611.1
CDS	1747..2586
Translation	MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

Target ORF information:

RefSeq Version	XM_011527309.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527309.3

ORF Insert Sequence:

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHF1 ( XM_006723378.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723441.1 Homo sapiens pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), transcript variant X2, mRNA.

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CloneID	OHu31082
Clone ID Related Accession (Same CDS sequence)	XM_011527309.2 , XM_011527309.3 , XM_006723378.1 , XM_011527309.1 , NM_024310.4 , NM_024310.5
Accession Version	XM_006723378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	pleckstrin homology domain-containing family F member 1 isoform X1
Comment

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723441.1
Translation	MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

Target ORF information:

RefSeq Version	XM_006723378.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723378.1

ORF Insert Sequence:

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHF1 ( XM_011527309.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525611.1 Homo sapiens pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), transcript variant X1, mRNA.

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CloneID	OHu31082
Clone ID Related Accession (Same CDS sequence)	XM_011527309.2 , XM_011527309.3 , XM_006723378.1 , XM_011527309.1 , NM_024310.4 , NM_024310.5
Accession Version	XM_011527309.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	pleckstrin homology domain-containing family F member 1 isoform X1
Comment

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525611.1
Translation	MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

Target ORF information:

RefSeq Version	XM_011527309.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527309.1

ORF Insert Sequence:

ATGGTGGACC ACTTGGCCAA CACGGAGATC AACAGCCAGC GCATCGCGGC AGTGGAGAGC 
TGCTTCGGGG CCTCGGGGCA GCCGCTGGCG CTGCCAGGCC GAGTGCTGCT GGGCGAGGGC 
GTGCTGACCA AAGAGTGCCG CAAGAAGGCC AAGCCGCGCA TCTTCTTCCT CTTTAACGAC 
ATCCTGGTGT ATGGCAGCAT CGTGCTCAAC AAGCGCAAGT ACCGCAGCCA GCACATCATC 
CCCCTGGAGG AGGTCACACT GGAGCTGTTG CCGGAGACGC TGCAGGCCAA GAACCGCTGG 
ATGATCAAGA CGGCCAAGAA GTCCTTTGTG GTGTCGGCCG CCTCCGCTAC GGAGCGCCAG 
GAATGGATTA GCCACATCGA GGAGTGCGTG CGGCGGCAAC TGAGGGCCAC GGGCCGCCCG 
CCCAGCACGG AGCACGCGGC ACCCTGGATC CCCGACAAGG CCACGGACAT CTGCATGCGC 
TGCACGCAGA CGCGCTTCTC TGCCCTCACG AGGCGCCACC ACTGCCGCAA GTGCGGCTTC 
GTGGTCTGCG CTGAGTGCTC GCGCCAGCGC TTCCTGCTCC CGCGCCTGTC CCCCAAGCCC 
GTGCGCGTCT GCAGCCTCTG CTACCGCGAA CTGGCCGCCC AGCAGCGGCA GGAGGAGGCG 
GAGGAGCAGG GCGCGGGGTC CCCAGGGCAG CCAGCCCACC TGGCCCGGCC CATCTGCGGA 
GCGTCCAGTG GAGATGACGA TGACTCCGAC GAGGACAAGG AGGGCAGCAG GGACGGCGAC 
TGGCCCAGCA GCGTGGAGTT CTACGCCTCG GGGGTGGCCT GGTCTGCCTT CCACAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHF1 ( XM_005259256.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005259313.1 Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), transcript variant X2, mRNA.

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CloneID	OHu52485
Clone ID Related Accession (Same CDS sequence)	XM_005259256.3
Accession Version	XM_005259256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pleckstrin homology domain-containing family F member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005259256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCGAG TCTCCCATCA CCACCCCCGG ACAAGCGAGC TCTCTCCCGC CGCGCGGTCT 
TGGCGGCTCC TGGGCCGGCG GGGCGCAGTG CCGCTGACTC CAGCCCAAGA AGAGGCCTGG 
GAAGGGCGCT CCTGGGCGGC TTGCGGGGTG AACCTGCAGG GAGCCTGGGG AGAGCGGTCA 
GGGGTCAGAG CGTCCGAGGC GGAATCCCCG GGGAAACGCG CAGATGTAAG CTGGTGGAGC 
CGCCAGCTGG AGACGATGGT GGACCACTTG GCCAACACGG AGATCAACAG CCAGCGCATC 
GCGGCAGTGG AGAGCTGCTT CGGGGCCTCG GGGCAGCCGC TGGCGCTGCC AGGCCGAGTG 
CTGCTGGGCG AGGGCGTGCT GACCAAAGAG TGCCGCAAGA AGGCCAAGCC GCGCATCTTC 
TTCCTCTTTA ACGACATCCT GGTGTATGGC AGCATCGTGC TCAACAAGCG CAAGTACCGC 
AGCCAGCACA TCATCCCCCT GGAGGAGGTC ACACTGGAGC TGTTGCCGGA GACGCTGCAG 
GCCAAGAACC GCTGGATGAT CAAGACGGCC AAGAAGTCCT TTGTGGTGTC GGCCGCCTCC 
GCTACGGAGC GCCAGGAATG GATTAGCCAC ATCGAGGAGT GCGTGCGGCG GCAACTGAGG 
GCCACGGGCC GCCCGCCCAG CACGGAGCAC GCGGCACCCT GGATCCCCGA CAAGGCCACG 
GACATCTGCA TGCGCTGCAC GCAGACGCGC TTCTCTGCCC TCACGAGGCG CCACCACTGC 
CGCAAGTGCG GCTTCGTGGT CTGCGCTGAG TGCTCGCGCC AGCGCTTCCT GCTCCCGCGC 
CTGTCCCCCA AGCCCGTGCG CGTCTGCAGC CTCTGCTACC GCGAACTGGC CGCCCAGCAG 
CGGCAGGAGG AGGCGGAGGA GCAGGGCGCG GGGTCCCCAG GGCAGCCAGC CCACCTGGCC 
CGGCCCATCT GCGGAGCGTC CAGTGGAGAT GACGATGACT CCGACGAGGA CAAGGAGGGC 
AGCAGGGACG GCGACTGGCC CAGCAGCGTG GAGTTCTACG CCTCGGGGGT GGCCTGGTCT 
GCCTTCCACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005259313.1
CDS	136..1230
Translation	MRRVSHHHPRTSELSPAARSWRLLGRRGAVPLTPAQEEAWEGRSWAACGVNLQGAWGERSGVRASEAESPGKRADVSWWSRQLETMVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS

Target ORF information:

RefSeq Version	XM_005259256.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pleckstrin homology and FYVE domain containing 1 (PLEKHF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259256.3

ORF Insert Sequence:

ATGAGGCGAG TCTCCCATCA CCACCCCCGG ACAAGCGAGC TCTCTCCCGC CGCGCGGTCT 
TGGCGGCTCC TGGGCCGGCG GGGCGCAGTG CCGCTGACTC CAGCCCAAGA AGAGGCCTGG 
GAAGGGCGCT CCTGGGCGGC TTGCGGGGTG AACCTGCAGG GAGCCTGGGG AGAGCGGTCA 
GGGGTCAGAG CGTCCGAGGC GGAATCCCCG GGGAAACGCG CAGATGTAAG CTGGTGGAGC 
CGCCAGCTGG AGACGATGGT GGACCACTTG GCCAACACGG AGATCAACAG CCAGCGCATC 
GCGGCAGTGG AGAGCTGCTT CGGGGCCTCG GGGCAGCCGC TGGCGCTGCC AGGCCGAGTG 
CTGCTGGGCG AGGGCGTGCT GACCAAAGAG TGCCGCAAGA AGGCCAAGCC GCGCATCTTC 
TTCCTCTTTA ACGACATCCT GGTGTATGGC AGCATCGTGC TCAACAAGCG CAAGTACCGC 
AGCCAGCACA TCATCCCCCT GGAGGAGGTC ACACTGGAGC TGTTGCCGGA GACGCTGCAG 
GCCAAGAACC GCTGGATGAT CAAGACGGCC AAGAAGTCCT TTGTGGTGTC GGCCGCCTCC 
GCTACGGAGC GCCAGGAATG GATTAGCCAC ATCGAGGAGT GCGTGCGGCG GCAACTGAGG 
GCCACGGGCC GCCCGCCCAG CACGGAGCAC GCGGCACCCT GGATCCCCGA CAAGGCCACG 
GACATCTGCA TGCGCTGCAC GCAGACGCGC TTCTCTGCCC TCACGAGGCG CCACCACTGC 
CGCAAGTGCG GCTTCGTGGT CTGCGCTGAG TGCTCGCGCC AGCGCTTCCT GCTCCCGCGC 
CTGTCCCCCA AGCCCGTGCG CGTCTGCAGC CTCTGCTACC GCGAACTGGC CGCCCAGCAG 
CGGCAGGAGG AGGCGGAGGA GCAGGGCGCG GGGTCCCCAG GGCAGCCAGC CCACCTGGCC 
CGGCCCATCT GCGGAGCGTC CAGTGGAGAT GACGATGACT CCGACGAGGA CAAGGAGGGC 
AGCAGGGACG GCGACTGGCC CAGCAGCGTG GAGTTCTACG CCTCGGGGGT GGCCTGGTCT 
GCCTTCCACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lysosomal targeting of phafin1 mediated by Rab7 induces autophagosome formation.
Biochemical and biophysical research communications417(1)35-42(2012 Jan)
Lin WJ,Yang CY,Li LL,Yi YH,Chen KW,Lin YC,Liu CC,Lin CH

The lysosome-associated apoptosis-inducing protein containing the pleckstrin homology (PH) and FYVE domains (LAPF), representative of a novel family of PH and FYVE domain-containing proteins, induces caspase-independent apoptosis via the lysosomal-mitochondrial pathway.
The Journal of biological chemistry280(49)40985-95(2005 Dec)
Chen W,Li N,Chen T,Han Y,Li C,Wang Y,He W,Zhang L,Wan T,Cao X

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PLEKHF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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