ALDH5A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALDH5A1
Entrez Gene ID	7915
Full Name	aldehyde dehydrogenase 5 family member A1
Synonyms	SSADH,SSDH
General protein information	Preferred Names aldehyde dehydrogenase 5 family member A1 Names succinate-semialdehyde dehydrogenase, mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase mitochondrial succinate semialdehyde dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p22.3
Summary	This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	610045
Disorder Html:	Succinic semialdehyde dehydrogenase deficiency, 271980 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001368954.1	NP_001355883.1	succinate-semialdehyde dehydrogenase, mitochondrial isoform 3
NM_001080.3	NP_001071.1	succinate-semialdehyde dehydrogenase, mitochondrial isoform 2 precursor
NM_170740.1	NP_733936.1	succinate-semialdehyde dehydrogenase, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ALDH5A1	NP_001071.1
	ALDH5A1	XP_001101142.1
Bos taurus (cattle)	ALDH5A1	NP_001179664.1
Mus musculus (house mouse)	Aldh5a1	NP_766120.1
Drosophila melanogaster (fruit fly)	Ssadh	NP_001014665.1
Magnaporthe oryzae (rice blast fungus)	MGG_01230	XP_003714132.1
Canis lupus familiaris (dog)	ALDH5A1	XP_005640135.1
Rattus norvegicus (Norway rat)	Aldh5a1	NP_074042.1
Gallus gallus (chicken)	ALDH5A1	XP_418909.3
Caenorhabditis elegans (roundworm)	alh-7	NP_001254393.1
Saccharomyces cerevisiae (baker's yeast)	UGA2	NP_009560.1
Kluyveromyces lactis	KLLA0E17491g	XP_454738.1
Eremothecium gossypii	AGOS_AER007W	NP_984867.1
Neurospora crassa	NCU00936	XP_964762.2
Danio rerio (zebrafish)	aldh5a1	NP_001103938.2
Schizosaccharomyces pombe (fission yeast)	SPAC1002.12c	NP_593499.2
Xenopus tropicalis (tropical clawed frog)	aldh5a1	NP_001135578.1
Pan troglodytes (chimpanzee)	ALDH5A1	NP_001008991.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003165	XP_312856.4
Arabidopsis thaliana (thale cress)	ALDH5F1	NP_178062.1

Gene Ontology
Bibliography

Related articles in PubMed

SSADH deficiency in an Italian family: a novel ALDH5A1 gene mutation affecting the succinic semialdehyde substrate binding site.
Leo S, Capo C, Ciminelli BM, Iacovelli F, Menduti G, Funghini S, Donati MA, Falconi M, Rossi L, Malaspina P
Metabolic brain disease32(5)1383-1388(2017 Oct)

Decreased expression of ALDH5A1 predicts prognosis in patients with ovarian cancer.
Tian X, Han Y, Yu L, Luo B, Hu Z, Li X, Yang Z, Wang X, Huang W, Wang H, Zhang Q, Ma D
Cancer biology & therapy18(4)245-251(2017 Apr)

[Identification of ALDH5A1 gene mutations in a Chinese family affected with succinic semialdehyde dehydrogenase deficiency].
Shu J, Cai F, Fan W, Meng Y, Zhang C, Cai C, Zhang Y, Lin S
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(1)6-9(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The proband was found to have compound heterozygous mutations of the succinate-semialdehyde dehydrogenase (ALDH5A1) gene, namely c.398_399delAA (p.N134X) and c.638G>T (p.R213L), for which her parents were both heterozygous carriers.
Title: [Identification of ALDH5A1 gene mutations in a Chinese family affected with succinic semialdehyde dehydrogenase deficiency].

Two novel ALDH5A1 mutations likely responsible for SSADH deficiency were identified, and DNA sequencing provided an accurate diagnosis for an at-risk fetus whose sibling had SSADH deficiency. Current study and literature review identified nine additional novel mutations in eight unrelated families bringing the number of unique mutations of ALDH5A1 resulting in SSADH deficiency to 44, occurring from exon 1 to exon 10.
Title: Mutation analysis and prenatal diagnosis in a Chinese family with succinic semialdehyde dehydrogenase and a systematic review of the literature of reported ALDH5A1 mutations.

SSADH catalytic loop role in the SSADH redox-switch modulation
Title: Modeling conformational redox-switch modulation of human succinic semialdehyde dehydrogenase.

Pearl et al. identify 3 new pathogenic mutations in the ALDH5A1 gene previously unreported in the literature. EXON: 1 Nucleotide change: c.412 C>T Change in protein: p.L138F EXON: 4 Nucleotide change: c.754G>T Change in protein: p.Q252X EXON: 8 Nucleotide change: c.1360G>A Change in protein: p.A454T
Title: Inherited disorders of gamma-aminobutyric acid metabolism and advances in ALDH5A1 mutation identification.

The strongest association signal arose from an intronic region of the gene ALDH5A1, which encodes the mitochondrial enzyme succinic semialdehyde dehydrogenase (SSADH), an enzyme involved in gamma-aminobutyric acid metabolism.
Title: Suggestive association with ocular phoria at chromosome 6p22.

The following ALDH5A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALDH5A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17139	NM_001080.3 Latest version!	Homo sapiens aldehyde dehydrogenase 5 family member A1 (ALDH5A1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu17871	NM_170740.1 Latest version!	Homo sapiens aldehyde dehydrogenase 5 family member A1 (ALDH5A1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu107987	NM_001368954.1 Latest version!	Homo sapiens aldehyde dehydrogenase 5 family member A1 (ALDH5A1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALDH5A1 ( NM_001080.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001071.1 Homo sapiens aldehyde dehydrogenase 5 family member A1 (ALDH5A1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17139
Clone ID Related Accession (Same CDS sequence)	NM_001080.3
Accession Version	NM_001080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	succinate-semialdehyde dehydrogenase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL031230.1. On Nov 27, 2002 this sequence version replaced NM_001080.2. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (2), that is missing an internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ427355.2, SRR1803615.223234.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000357578.8/ ENSP00000350191.3 RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACCT GCATTTGGCT GCGGAGCTGT GGGGCCCGGC GCCTCGGGTC GACGTTTCCA 
GGCTGCCGCC TCCGCCCCCG CGCCGGCGGC CTGGTCCCTG CCTCCGGGCC TGCGCCCGGC 
CCGGCCCAGC TCCGCTGCTA CGCTGGGCGC CTGGCGGGCC TCTCTGCGGC GCTGCTGCGC 
ACCGACAGCT TCGTGGGCGG CCGCTGGCTC CCGGCCGCCG CCACCTTCCC CGTGCAAGAC 
CCGGCCAGCG GCGCCGCTCT GGGCATGGTA GCCGACTGCG GGGTGCGAGA GGCCCGCGCC 
GCCGTGCGCG CTGCCTACGA GGCTTTCTGC CGCTGGAGGG AGGTCTCCGC CAAGGAGAGG 
AGTTCATTAC TTCGGAAGTG GTACAATTTA ATGATACAAA ATAAGGATGA CCTTGCCAGA 
ATAATCACAG CTGAAAGTGG AAAGCCACTG AAGGAGGCAC ATGGAGAAAT TCTCTATTCC 
GCCTTTTTCC TAGAGTGGTT CTCTGAGGAA GCCCGCCGTG TTTACGGAGA CATTATCCAC 
ACCCCGGCAA AGGACAGGCG GGCCCTGGTC CTCAAGCAGC CCATAGGCGT GGCTGCAGTC 
ATCACCCCGT GGAATTTCCC CAGTGCCATG ATCACCCGGA AGGTGGGGGC CGCCCTGGCA 
GCCGGCTGTA CTGTCGTGGT GAAGCCTGCC GAAGACACGC CCTTCTCCGC CCTGGCCCTG 
GCTGAGCTTG CAAGCCAGGC TGGGATTCCT TCAGGTGTAT ACAATGTTAT TCCCTGTTCT 
CGAAAGAATG CCAAGGAAGT AGGGGAGGCA ATTTGTACTG ATCCTCTGGT GTCCAAAATT 
TCCTTTACTG GTTCAACAAC TACAGGAAAG ATCCTGTTGC ACCACGCAGC AAACTCTGTG 
AAAAGGGTCT CTATGGAGCT GGGCGGCCTT GCTCCATTTA TAGTATTTGA CAGTGCCAAC 
GTGGACCAGG CTGTAGCAGG GGCCATGGCA TCTAAATTTA GGAACACTGG ACAGACTTGT 
GTTTGCTCAA ACCAATTCTT GGTGCAAAGG GGCATCCATG ATGCCTTTGT AAAAGCATTC 
GCCGAGGCCA TGAAGAAGAA CCTGCGCGTA GGTAATGGAT TTGAGGAAGG AACTACTCAG 
GGCCCATTAA TTAATGAAAA AGCGGTAGAA AAGGTGGAGA AACAGGTGAA TGATGCCGTT 
TCTAAAGGTG CCACCGTTGT GACAGGTGGA AAACGACACC AACTTGGAAA AAATTTCTTT 
GAGCCTACCC TGCTGTGCAA TGTCACCCAG GACATGCTGT GCACTCATGA AGAGACTTTC 
GGGCCTCTGG CACCAGTTAT CAAGTTCGAT ACAGAGGAGG AGGCTATAGC AATCGCTAAC 
GCAGCTGATG TTGGGTTAGC AGGTTATTTT TACTCTCAAG ACCCAGCCCA GATCTGGAGA 
GTGGCAGAGC AGCTGGAAGT GGGCATGGTT GGCGTCAACG AAGGATTAAT TTCCTCTGTG 
GAGTGCCCTT TTGGTGGAGT GAAGCAGTCC GGCCTTGGGC GAGAGGGGTC CAAGTATGGC 
ATTGATGAGT ATCTGGAACT CAAGTATGTG TGTTACGGGG GCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071.1
CDS	29..1636
Translation	MATCIWLRSCGARRLGSTFPGCRLRPRAGGLVPASGPAPGPAQLRCYAGRLAGLSAALLRTDSFVGGRWLPAAATFPVQDPASGAALGMVADCGVREARAAVRAAYEAFCRWREVSAKERSSLLRKWYNLMIQNKDDLARIITAESGKPLKEAHGEILYSAFFLEWFSEEARRVYGDIIHTPAKDRRALVLKQPIGVAAVITPWNFPSAMITRKVGAALAAGCTVVVKPAEDTPFSALALAELASQAGIPSGVYNVIPCSRKNAKEVGEAICTDPLVSKISFTGSTTTGKILLHHAANSVKRVSMELGGLAPFIVFDSANVDQAVAGAMASKFRNTGQTCVCSNQFLVQRGIHDAFVKAFAEAMKKNLRVGNGFEEGTTQGPLINEKAVEKVEKQVNDAVSKGATVVTGGKRHQLGKNFFEPTLLCNVTQDMLCTHEETFGPLAPVIKFDTEEEAIAIANAADVGLAGYFYSQDPAQIWRVAEQLEVGMVGVNEGLISSVECPFGGVKQSGLGREGSKYGIDEYLELKYVCYGGL

Target ORF information:

RefSeq Version	NM_001080.3
Organism	Homo sapiens(human)
Definition	Homo sapiens aldehyde dehydrogenase 5 family member A1 (ALDH5A1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080.3

ORF Insert Sequence:

ATGGCGACCT GCATTTGGCT GCGGAGCTGT GGGGCCCGGC GCCTCGGGTC GACGTTTCCA 
GGCTGCCGCC TCCGCCCCCG CGCCGGCGGC CTGGTCCCTG CCTCCGGGCC TGCGCCCGGC 
CCGGCCCAGC TCCGCTGCTA CGCTGGGCGC CTGGCGGGCC TCTCTGCGGC GCTGCTGCGC 
ACCGACAGCT TCGTGGGCGG CCGCTGGCTC CCGGCCGCCG CCACCTTCCC CGTGCAAGAC 
CCGGCCAGCG GCGCCGCTCT GGGCATGGTA GCCGACTGCG GGGTGCGAGA GGCCCGCGCC 
GCCGTGCGCG CTGCCTACGA GGCTTTCTGC CGCTGGAGGG AGGTCTCCGC CAAGGAGAGG 
AGTTCATTAC TTCGGAAGTG GTACAATTTA ATGATACAAA ATAAGGATGA CCTTGCCAGA 
ATAATCACAG CTGAAAGTGG AAAGCCACTG AAGGAGGCAC ATGGAGAAAT TCTCTATTCC 
GCCTTTTTCC TAGAGTGGTT CTCTGAGGAA GCCCGCCGTG TTTACGGAGA CATTATCCAC 
ACCCCGGCAA AGGACAGGCG GGCCCTGGTC CTCAAGCAGC CCATAGGCGT GGCTGCAGTC 
ATCACCCCGT GGAATTTCCC CAGTGCCATG ATCACCCGGA AGGTGGGGGC CGCCCTGGCA 
GCCGGCTGTA CTGTCGTGGT GAAGCCTGCC GAAGACACGC CCTTCTCCGC CCTGGCCCTG 
GCTGAGCTTG CAAGCCAGGC TGGGATTCCT TCAGGTGTAT ACAATGTTAT TCCCTGTTCT 
CGAAAGAATG CCAAGGAAGT AGGGGAGGCA ATTTGTACTG ATCCTCTGGT GTCCAAAATT 
TCCTTTACTG GTTCAACAAC TACAGGAAAG ATCCTGTTGC ACCACGCAGC AAACTCTGTG 
AAAAGGGTCT CTATGGAGCT GGGCGGCCTT GCTCCATTTA TAGTATTTGA CAGTGCCAAC 
GTGGACCAGG CTGTAGCAGG GGCCATGGCA TCTAAATTTA GGAACACTGG ACAGACTTGT 
GTTTGCTCAA ACCAATTCTT GGTGCAAAGG GGCATCCATG ATGCCTTTGT AAAAGCATTC 
GCCGAGGCCA TGAAGAAGAA CCTGCGCGTA GGTAATGGAT TTGAGGAAGG AACTACTCAG 
GGCCCATTAA TTAATGAAAA AGCGGTAGAA AAGGTGGAGA AACAGGTGAA TGATGCCGTT 
TCTAAAGGTG CCACCGTTGT GACAGGTGGA AAACGACACC AACTTGGAAA AAATTTCTTT 
GAGCCTACCC TGCTGTGCAA TGTCACCCAG GACATGCTGT GCACTCATGA AGAGACTTTC 
GGGCCTCTGG CACCAGTTAT CAAGTTCGAT ACAGAGGAGG AGGCTATAGC AATCGCTAAC 
GCAGCTGATG TTGGGTTAGC AGGTTATTTT TACTCTCAAG ACCCAGCCCA GATCTGGAGA 
GTGGCAGAGC AGCTGGAAGT GGGCATGGTT GGCGTCAACG AAGGATTAAT TTCCTCTGTG 
GAGTGCCCTT TTGGTGGAGT GAAGCAGTCC GGCCTTGGGC GAGAGGGGTC CAAGTATGGC 
ATTGATGAGT ATCTGGAACT CAAGTATGTG TGTTACGGGG GCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALDH5A1 ( NM_170740.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_733936.1 Homo sapiens aldehyde dehydrogenase 5 family member A1 (ALDH5A1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17871
Clone ID Related Accession (Same CDS sequence)	NM_170740.1
Accession Version	NM_170740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	succinate-semialdehyde dehydrogenase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL031230.1. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ427354.2, KJ534769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACCT GCATTTGGCT GCGGAGCTGT GGGGCCCGGC GCCTCGGGTC GACGTTTCCA 
GGCTGCCGCC TCCGCCCCCG CGCCGGCGGC CTGGTCCCTG CCTCCGGGCC TGCGCCCGGC 
CCGGCCCAGC TCCGCTGCTA CGCTGGGCGC CTGGCGGGCC TCTCTGCGGC GCTGCTGCGC 
ACCGACAGCT TCGTGGGCGG CCGCTGGCTC CCGGCCGCCG CCACCTTCCC CGTGCAAGAC 
CCGGCCAGCG GCGCCGCTCT GGGCATGGTA GCCGACTGCG GGGTGCGAGA GGCCCGCGCC 
GCCGTGCGCG CTGCCTACGA GGCTTTCTGC CGCTGGAGGG AGGTCTCCGC CAAGGAGAGG 
AGTTCATTAC TTCGGAAGTG GTACAATTTA ATGATACAAA ATAAGGATGA CCTTGCCAGA 
ATAATCACAG CTGAAAGTGG AAAGCCACTG AAGGAGGCAC ATGGAGAAAT TCTCTATTCC 
GCCTTTTTCC TAGAGTGGTT CTCTGAGGAA GCCCGCCGTG TTTACGGAGA CATTATCCAC 
ACCCCGGCAA AGGACAGGCG GGCCCTGGTC CTCAAGCAGC CCATAGGCGT GGCTGCAGTC 
ATCACCCCGT GGAATTTCCC CAGTGCCATG ATCACCCGGA AGGTGGGGGC CGCCCTGGCA 
GCCGGCTGTA CTGTCGTGGT GAAGCCTGCC GAAGACACGC CCTTCTCCGC CCTGGCCCTG 
GCTGAGGTAA ATCAGGGATT TCTTCTTGAT TTGGATCCAT TGTTGCTTGC AAGCCAGGCT 
GGGATTCCTT CAGGTGTATA CAATGTTATT CCCTGTTCTC GAAAGAATGC CAAGGAAGTA 
GGGGAGGCAA TTTGTACTGA TCCTCTGGTG TCCAAAATTT CCTTTACTGG TTCAACAACT 
ACAGGAAAGA TCCTGTTGCA CCACGCAGCA AACTCTGTGA AAAGGGTCTC TATGGAGCTG 
GGCGGCCTTG CTCCATTTAT AGTATTTGAC AGTGCCAACG TGGACCAGGC TGTAGCAGGG 
GCCATGGCAT CTAAATTTAG GAACACTGGA CAGACTTGTG TTTGCTCAAA CCAATTCTTG 
GTGCAAAGGG GCATCCATGA TGCCTTTGTA AAAGCATTCG CCGAGGCCAT GAAGAAGAAC 
CTGCGCGTAG GTAATGGATT TGAGGAAGGA ACTACTCAGG GCCCATTAAT TAATGAAAAA 
GCGGTAGAAA AGGTGGAGAA ACAGGTGAAT GATGCCGTTT CTAAAGGTGC CACCGTTGTG 
ACAGGTGGAA AACGACACCA ACTTGGAAAA AATTTCTTTG AGCCTACCCT GCTGTGCAAT 
GTCACCCAGG ACATGCTGTG CACTCATGAA GAGACTTTCG GGCCTCTGGC ACCAGTTATC 
AAGTTCGATA CAGAGGAGGA GGCTATAGCA ATCGCTAACG CAGCTGATGT TGGGTTAGCA 
GGTTATTTTT ACTCTCAAGA CCCAGCCCAG ATCTGGAGAG TGGCAGAGCA GCTGGAAGTG 
GGCATGGTTG GCGTCAACGA AGGATTAATT TCCTCTGTGG AGTGCCCTTT TGGTGGAGTG 
AAGCAGTCCG GCCTTGGGCG AGAGGGGTCC AAGTATGGCA TTGATGAGTA TCTGGAACTC 
AAGTATGTGT GTTACGGGGG CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733936.1
CDS	29..1675
Translation	MATCIWLRSCGARRLGSTFPGCRLRPRAGGLVPASGPAPGPAQLRCYAGRLAGLSAALLRTDSFVGGRWLPAAATFPVQDPASGAALGMVADCGVREARAAVRAAYEAFCRWREVSAKERSSLLRKWYNLMIQNKDDLARIITAESGKPLKEAHGEILYSAFFLEWFSEEARRVYGDIIHTPAKDRRALVLKQPIGVAAVITPWNFPSAMITRKVGAALAAGCTVVVKPAEDTPFSALALAEVNQGFLLDLDPLLLASQAGIPSGVYNVIPCSRKNAKEVGEAICTDPLVSKISFTGSTTTGKILLHHAANSVKRVSMELGGLAPFIVFDSANVDQAVAGAMASKFRNTGQTCVCSNQFLVQRGIHDAFVKAFAEAMKKNLRVGNGFEEGTTQGPLINEKAVEKVEKQVNDAVSKGATVVTGGKRHQLGKNFFEPTLLCNVTQDMLCTHEETFGPLAPVIKFDTEEEAIAIANAADVGLAGYFYSQDPAQIWRVAEQLEVGMVGVNEGLISSVECPFGGVKQSGLGREGSKYGIDEYLELKYVCYGGL

Target ORF information:

RefSeq Version	NM_170740.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aldehyde dehydrogenase 5 family member A1 (ALDH5A1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170740.1

ORF Insert Sequence:

ATGGCGACCT GCATTTGGCT GCGGAGCTGT GGGGCCCGGC GCCTCGGGTC GACGTTTCCA 
GGCTGCCGCC TCCGCCCCCG CGCCGGCGGC CTGGTCCCTG CCTCCGGGCC TGCGCCCGGC 
CCGGCCCAGC TCCGCTGCTA CGCTGGGCGC CTGGCGGGCC TCTCTGCGGC GCTGCTGCGC 
ACCGACAGCT TCGTGGGCGG CCGCTGGCTC CCGGCCGCCG CCACCTTCCC CGTGCAAGAC 
CCGGCCAGCG GCGCCGCTCT GGGCATGGTA GCCGACTGCG GGGTGCGAGA GGCCCGCGCC 
GCCGTGCGCG CTGCCTACGA GGCTTTCTGC CGCTGGAGGG AGGTCTCCGC CAAGGAGAGG 
AGTTCATTAC TTCGGAAGTG GTACAATTTA ATGATACAAA ATAAGGATGA CCTTGCCAGA 
ATAATCACAG CTGAAAGTGG AAAGCCACTG AAGGAGGCAC ATGGAGAAAT TCTCTATTCC 
GCCTTTTTCC TAGAGTGGTT CTCTGAGGAA GCCCGCCGTG TTTACGGAGA CATTATCCAC 
ACCCCGGCAA AGGACAGGCG GGCCCTGGTC CTCAAGCAGC CCATAGGCGT GGCTGCAGTC 
ATCACCCCGT GGAATTTCCC CAGTGCCATG ATCACCCGGA AGGTGGGGGC CGCCCTGGCA 
GCCGGCTGTA CTGTCGTGGT GAAGCCTGCC GAAGACACGC CCTTCTCCGC CCTGGCCCTG 
GCTGAGGTAA ATCAGGGATT TCTTCTTGAT TTGGATCCAT TGTTGCTTGC AAGCCAGGCT 
GGGATTCCTT CAGGTGTATA CAATGTTATT CCCTGTTCTC GAAAGAATGC CAAGGAAGTA 
GGGGAGGCAA TTTGTACTGA TCCTCTGGTG TCCAAAATTT CCTTTACTGG TTCAACAACT 
ACAGGAAAGA TCCTGTTGCA CCACGCAGCA AACTCTGTGA AAAGGGTCTC TATGGAGCTG 
GGCGGCCTTG CTCCATTTAT AGTATTTGAC AGTGCCAACG TGGACCAGGC TGTAGCAGGG 
GCCATGGCAT CTAAATTTAG GAACACTGGA CAGACTTGTG TTTGCTCAAA CCAATTCTTG 
GTGCAAAGGG GCATCCATGA TGCCTTTGTA AAAGCATTCG CCGAGGCCAT GAAGAAGAAC 
CTGCGCGTAG GTAATGGATT TGAGGAAGGA ACTACTCAGG GCCCATTAAT TAATGAAAAA 
GCGGTAGAAA AGGTGGAGAA ACAGGTGAAT GATGCCGTTT CTAAAGGTGC CACCGTTGTG 
ACAGGTGGAA AACGACACCA ACTTGGAAAA AATTTCTTTG AGCCTACCCT GCTGTGCAAT 
GTCACCCAGG ACATGCTGTG CACTCATGAA GAGACTTTCG GGCCTCTGGC ACCAGTTATC 
AAGTTCGATA CAGAGGAGGA GGCTATAGCA ATCGCTAACG CAGCTGATGT TGGGTTAGCA 
GGTTATTTTT ACTCTCAAGA CCCAGCCCAG ATCTGGAGAG TGGCAGAGCA GCTGGAAGTG 
GGCATGGTTG GCGTCAACGA AGGATTAATT TCCTCTGTGG AGTGCCCTTT TGGTGGAGTG 
AAGCAGTCCG GCCTTGGGCG AGAGGGGTCC AAGTATGGCA TTGATGAGTA TCTGGAACTC 
AAGTATGTGT GTTACGGGGG CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALDH5A1 ( NM_001368954.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001355883.1 Homo sapiens aldehyde dehydrogenase 5 family member A1 (ALDH5A1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu107987
Clone ID Related Accession (Same CDS sequence)	NM_001368954.1
Accession Version	NM_001368954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	succinate-semialdehyde dehydrogenase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL031230.1. Transcript Variant: This variant (3) lacks two consecutive, in-frame, alternate exons, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGACCT GCATTTGGCT GCGGAGCTGT GGGGCCCGGC GCCTCGGGTC GACGTTTCCA 
GGCTGCCGCC TCCGCCCCCG CGCCGGCGGC CTGGTCCCTG CCTCCGGGCC TGCGCCCGGC 
CCGGCCCAGC TCCGCTGCTA CGCTGGGCGC CTGGCGGGCC TCTCTGCGGC GCTGCTGCGC 
ACCGACAGCT TCGTGGGCGG CCGCTGGCTC CCGGCCGCCG CCACCTTCCC CGTGCAAGAC 
CCGGCCAGCG GCGCCGCTCT GGGCATGGTA GCCGACTGCG GGGTGCGAGA GGCCCGCGCC 
GCCGTGCGCG CTGCCTACGA GGCTTTCTGC CGCTGGAGGG AGGTCTCCGC CAAGGAGAGG 
AGTTCATTAC TTCGGAAGTG GTACAATTTA ATGATACAAA ATAAGGATGA CCTTGCCAGA 
ATAATCACAG CTGAAAGTGG AAAGCCACTG AAGGAGGCAC ATGGAGAAAT TCTCTATTCC 
GCCTTTTTCC TAGAGTGGTT CTCTGAGGAA GCCCGCCGTG TTTACGGAGA CATTATCCAC 
ACCCCGGCAA AGGACAGGCG GGCCCTGGTC CTCAAGCAGC CCATAGGCGT GGCTGCAGTC 
ATCACCCCGT GGAATTTCCC CAGTGCCATG ATCACCCGGA AGGTGGGGGC CGCCCTGGCA 
GCCGGCTGTA CTGTCGTGGT GAAGCCTGCC GAAGACACGC CCTTCTCCGC CCTGGCCCTG 
GCTGAGATCC TGTTGCACCA CGCAGCAAAC TCTGTGAAAA GGGTCTCTAT GGAGCTGGGC 
GGCCTTGCTC CATTTATAGT ATTTGACAGT GCCAACGTGG ACCAGGCTGT AGCAGGGGCC 
ATGGCATCTA AATTTAGGAA CACTGGACAG ACTTGTGTTT GCTCAAACCA ATTCTTGGTG 
CAAAGGGGCA TCCATGATGC CTTTGTAAAA GCATTCGCCG AGGCCATGAA GAAGAACCTG 
CGCGTAGGTA ATGGATTTGA GGAAGGAACT ACTCAGGGCC CATTAATTAA TGAAAAAGCG 
GTAGAAAAGG TGGAGAAACA GGTGAATGAT GCCGTTTCTA AAGGTGCCAC CGTTGTGACA 
GGTGGAAAAC GACACCAACT TGGAAAAAAT TTCTTTGAGC CTACCCTGCT GTGCAATGTC 
ACCCAGGACA TGCTGTGCAC TCATGAAGAG ACTTTCGGGC CTCTGGCACC AGTTATCAAG 
TTCGATACAG AGGAGGAGGC TATAGCAATC GCTAACGCAG CTGATGTTGG GTTAGCAGGT 
TATTTTTACT CTCAAGACCC AGCCCAGATC TGGAGAGTGG CAGAGCAGCT GGAAGTGGGC 
ATGGTTGGCG TCAACGAAGG ATTAATTTCC TCTGTGGAGT GCCCTTTTGG TGGAGTGAAG 
CAGTCCGGCC TTGGGCGAGA GGGGTCCAAG TATGGCATTG ATGAGTATCT GGAACTCAAG 
TATGTGTGTT ACGGGGGCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355883.1
CDS	29..1492
Translation	MATCIWLRSCGARRLGSTFPGCRLRPRAGGLVPASGPAPGPAQLRCYAGRLAGLSAALLRTDSFVGGRWLPAAATFPVQDPASGAALGMVADCGVREARAAVRAAYEAFCRWREVSAKERSSLLRKWYNLMIQNKDDLARIITAESGKPLKEAHGEILYSAFFLEWFSEEARRVYGDIIHTPAKDRRALVLKQPIGVAAVITPWNFPSAMITRKVGAALAAGCTVVVKPAEDTPFSALALAEILLHHAANSVKRVSMELGGLAPFIVFDSANVDQAVAGAMASKFRNTGQTCVCSNQFLVQRGIHDAFVKAFAEAMKKNLRVGNGFEEGTTQGPLINEKAVEKVEKQVNDAVSKGATVVTGGKRHQLGKNFFEPTLLCNVTQDMLCTHEETFGPLAPVIKFDTEEEAIAIANAADVGLAGYFYSQDPAQIWRVAEQLEVGMVGVNEGLISSVECPFGGVKQSGLGREGSKYGIDEYLELKYVCYGGL

Target ORF information:

RefSeq Version	NM_001368954.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aldehyde dehydrogenase 5 family member A1 (ALDH5A1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368954.1

ORF Insert Sequence:

ATGGCGACCT GCATTTGGCT GCGGAGCTGT GGGGCCCGGC GCCTCGGGTC GACGTTTCCA 
GGCTGCCGCC TCCGCCCCCG CGCCGGCGGC CTGGTCCCTG CCTCCGGGCC TGCGCCCGGC 
CCGGCCCAGC TCCGCTGCTA CGCTGGGCGC CTGGCGGGCC TCTCTGCGGC GCTGCTGCGC 
ACCGACAGCT TCGTGGGCGG CCGCTGGCTC CCGGCCGCCG CCACCTTCCC CGTGCAAGAC 
CCGGCCAGCG GCGCCGCTCT GGGCATGGTA GCCGACTGCG GGGTGCGAGA GGCCCGCGCC 
GCCGTGCGCG CTGCCTACGA GGCTTTCTGC CGCTGGAGGG AGGTCTCCGC CAAGGAGAGG 
AGTTCATTAC TTCGGAAGTG GTACAATTTA ATGATACAAA ATAAGGATGA CCTTGCCAGA 
ATAATCACAG CTGAAAGTGG AAAGCCACTG AAGGAGGCAC ATGGAGAAAT TCTCTATTCC 
GCCTTTTTCC TAGAGTGGTT CTCTGAGGAA GCCCGCCGTG TTTACGGAGA CATTATCCAC 
ACCCCGGCAA AGGACAGGCG GGCCCTGGTC CTCAAGCAGC CCATAGGCGT GGCTGCAGTC 
ATCACCCCGT GGAATTTCCC CAGTGCCATG ATCACCCGGA AGGTGGGGGC CGCCCTGGCA 
GCCGGCTGTA CTGTCGTGGT GAAGCCTGCC GAAGACACGC CCTTCTCCGC CCTGGCCCTG 
GCTGAGATCC TGTTGCACCA CGCAGCAAAC TCTGTGAAAA GGGTCTCTAT GGAGCTGGGC 
GGCCTTGCTC CATTTATAGT ATTTGACAGT GCCAACGTGG ACCAGGCTGT AGCAGGGGCC 
ATGGCATCTA AATTTAGGAA CACTGGACAG ACTTGTGTTT GCTCAAACCA ATTCTTGGTG 
CAAAGGGGCA TCCATGATGC CTTTGTAAAA GCATTCGCCG AGGCCATGAA GAAGAACCTG 
CGCGTAGGTA ATGGATTTGA GGAAGGAACT ACTCAGGGCC CATTAATTAA TGAAAAAGCG 
GTAGAAAAGG TGGAGAAACA GGTGAATGAT GCCGTTTCTA AAGGTGCCAC CGTTGTGACA 
GGTGGAAAAC GACACCAACT TGGAAAAAAT TTCTTTGAGC CTACCCTGCT GTGCAATGTC 
ACCCAGGACA TGCTGTGCAC TCATGAAGAG ACTTTCGGGC CTCTGGCACC AGTTATCAAG 
TTCGATACAG AGGAGGAGGC TATAGCAATC GCTAACGCAG CTGATGTTGG GTTAGCAGGT 
TATTTTTACT CTCAAGACCC AGCCCAGATC TGGAGAGTGG CAGAGCAGCT GGAAGTGGGC 
ATGGTTGGCG TCAACGAAGG ATTAATTTCC TCTGTGGAGT GCCCTTTTGG TGGAGTGAAG 
CAGTCCGGCC TTGGGCGAGA GGGGTCCAAG TATGGCATTG ATGAGTATCT GGAACTCAAG 
TATGTGTGTT ACGGGGGCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SSADH deficiency in an Italian family: a novel ALDH5A1 gene mutation affecting the succinic semialdehyde substrate binding site.
Metabolic brain disease32(5)1383-1388(2017 Oct)
Leo S,Capo C,Ciminelli BM,Iacovelli F,Menduti G,Funghini S,Donati MA,Falconi M,Rossi L,Malaspina P

Decreased expression of ALDH5A1 predicts prognosis in patients with ovarian cancer.
Cancer biology & therapy18(4)245-251(2017 Apr)
Tian X,Han Y,Yu L,Luo B,Hu Z,Li X,Yang Z,Wang X,Huang W,Wang H,Zhang Q,Ma D

[Identification of ALDH5A1 gene mutations in a Chinese family affected with succinic semialdehyde dehydrogenase deficiency].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(1)6-9(2017 Feb)
Shu J,Cai F,Fan W,Meng Y,Zhang C,Cai C,Zhang Y,Lin S

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ALDH5A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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