Aldh5a1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aldh5a1
Entrez Gene ID	214579
Full Name	aldhehyde dehydrogenase family 5, subfamily A1
Synonyms	6330403E24Rik,Ahd-1,Ahd1,D630032B01Rik,SSADH,Ssdh1
General protein information	Preferred Names aldhehyde dehydrogenase family 5, subfamily A1 Names succinate-semialdehyde dehydrogenase, mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase aldehyde dehydrogenase 1, mitochondrial aldehyde dehydrogenase family 5 member A1 aldehyde dehydrogenase family 5, subfamily A1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 A3.1\|13 10.77 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_172532.3	NP_766120.1	succinate-semialdehyde dehydrogenase, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ALDH5A1	NP_001071.1
	ALDH5A1	XP_001101142.1
Bos taurus (cattle)	ALDH5A1	NP_001179664.1
Mus musculus (house mouse)	Aldh5a1	NP_766120.1
Drosophila melanogaster (fruit fly)	Ssadh	NP_001014665.1
Magnaporthe oryzae (rice blast fungus)	MGG_01230	XP_003714132.1
Canis lupus familiaris (dog)	ALDH5A1	XP_005640135.1
Rattus norvegicus (Norway rat)	Aldh5a1	NP_074042.1
Gallus gallus (chicken)	ALDH5A1	XP_418909.3
Caenorhabditis elegans (roundworm)	alh-7	NP_001254393.1
Saccharomyces cerevisiae (baker's yeast)	UGA2	NP_009560.1
Kluyveromyces lactis	KLLA0E17491g	XP_454738.1
Eremothecium gossypii	AGOS_AER007W	NP_984867.1
Neurospora crassa	NCU00936	XP_964762.2
Danio rerio (zebrafish)	aldh5a1	NP_001103938.2
Schizosaccharomyces pombe (fission yeast)	SPAC1002.12c	NP_593499.2
Xenopus tropicalis (tropical clawed frog)	aldh5a1	NP_001135578.1
Pan troglodytes (chimpanzee)	ALDH5A1	NP_001008991.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003165	XP_312856.4
Arabidopsis thaliana (thale cress)	ALDH5F1	NP_178062.1

Gene Ontology
Bibliography

Related articles in PubMed

In vitro modeling of experimental succinic semialdehyde dehydrogenase deficiency (SSADHD) using brain-derived neural stem cells.
Vogel KR, Ainslie GR, Jansen EE, Salomons GS, Roullet JB, Gibson KM
PloS one12(10)e0186919(2017)

Therapeutic relevance of mTOR inhibition in murine succinate semialdehyde dehydrogenase deficiency (SSADHD), a disorder of GABA metabolism.
Vogel KR, Ainslie GR, Jansen EE, Salomons GS, Gibson KM
Biochimica et biophysica acta1863(1)33-42(2017 Jan)

Defects in GABA metabolism affect selective autophagy pathways and are alleviated by mTOR inhibition.
Lakhani R, Vogel KR, Till A, Liu J, Burnett SF, Gibson KM, Subramani S
EMBO molecular medicine6(4)551-66(2014 04)

Aberrant GABA(A) receptor-mediated inhibition in cortico-thalamic networks of succinic semialdehyde dehydrogenase deficient mice.
Errington AC, Gibson KM, Crunelli V, Cope DW
PloS one6(4)e19021(2011 Apr)

Plasticity of postsynaptic, but not presynaptic, GABAB receptors in SSADH deficient mice.
Vardya I, Drasbek KR, Gibson KM, Jensen K
Experimental neurology225(1)114-22(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NSC model of SSADHD will be valuable in providing a first-tier screen for centrally-acting therapeutics and prioritizing therapeutic concepts of preclinical animal studies applicable to SSADHD
Title: In vitro modeling of experimental succinic semialdehyde dehydrogenase deficiency (SSADHD) using brain-derived neural stem cells.

These data show that enhanced, rather than compromised, GABA(A) receptor-mediated inhibition occurs in cortico-thalamic networks of SSADH(-/-) mice.
Title: Aberrant GABA(A) receptor-mediated inhibition in cortico-thalamic networks of succinic semialdehyde dehydrogenase deficient mice.

SSADH deficiency leads to elevated extracellular GABA levels and increased GABAergic neurotransmission in the mouse cerebral cortex
Title: SSADH deficiency leads to elevated extracellular GABA levels and increased GABAergic neurotransmission in the mouse cerebral cortex.

Data showing an imbalance between tissue antioxidant defences and oxidative attack strongly indicate that oxidative stress is involved in the pathophysiology of Adlh5a1 deficiency in mice.
Title: Evidence for oxidative stress in tissues derived from succinate semialdehyde dehydrogenase-deficient mice.

Antiepileptic agents agents are studied ro their effectiveness in knockout mice.
Title: Therapeutic concepts in succinate semialdehyde dehydrogenase (SSADH; ALDH5a1) deficiency (gamma-hydroxybutyric aciduria). Hypotheses evolved from 25 years of patient evaluation, studies in Aldh5a1-/- mice and characterization of gamma-hydroxybutyric acid pharmacology.

The following Aldh5a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aldh5a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15588	NM_172532.3 Latest version!	Mus musculus aldhehyde dehydrogenase family 5, subfamily A1 (Aldh5a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aldh5a1 ( NM_172532.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766120.1 Mus musculus aldhehyde dehydrogenase family 5, subfamily A1 (Aldh5a1), mRNA.

Price & Availability↑

CloneID	OMu15588
Clone ID Related Accession (Same CDS sequence)	NM_172532.3
Accession Version	NM_172532.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	succinate-semialdehyde dehydrogenase, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK052703.1, AK144030.1, AK164287.1 and CB723582.1. On Dec 19, 2009 this sequence version replaced NM_172532.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK144030.1, AK166313.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGACCT GCTTTCTGCT GCGGAGCTTC TGGGCCGCGC GTCCTGCGCT GCCGCCTCCC 
GGCCGCTTCC GCCCGGAGCC CGCAGGAACC CCGCGGCGCA GCTACGCCAG CGGCCCCGGG 
GGCCTCCACG CCGACCTACT CCGCGGGGAC AGCTTCGTGG GCGGCCGCTG GCTCCCAGCC 
CCCGCCACCT TCCCGGTGTA CGACCCGGCC AGCGGCGCCA AGCTGGGCAC CGTGGCCGAC 
TGCGGGGTAC CCGAGGCCCG CGCTGCTGTG CGCGCCGCCT ACGATGCCTT CAACAGCTGG 
AAAGGGGTCT CGGTCAAGGA GAGGAGCTTA CTACTCCGTA AATGGTACGA CTTAATGATA 
CAAAATAAGG ACGACCTTGC CAAGATCATA ACAGCCGAGA GTGGAAAGCC ACTGAAAGAG 
GCCCAGGGAG AAATTCTGTA TTCAGCCCTT TTCCTGGAGT GGTTCTCAGA GGAAGCTCGC 
CGTATTTATG GAGACATCAT CTATACCTCT GCCAAAGATA AGCGAGGGCT AGTCCTCAAG 
CAGCCCGTTG GTGTGGCGGC CATCATCACA CCGTGGAATT TTCCCAGTGC GATGATCACC 
CGGAAAGTGG GGGCCGCCTT GGCAGCTGGC TGCACTGTGG TGGTGAAACC TGCTGAAGAC 
ACACCCTATT CCGCCCTGGC CCTGGCTCAG CTTGCAAACC AGGCGGGAAT CCCTGCAGGT 
GTCTACAACG TCATTCCCTG CTCTAGAAAC AAGGCCAAGG AAGTGGGAGA GGTCCTGTGT 
ACCGATCCAT TGGTGTCCAA AATTTCCTTT ACTGGCTCAA CAGCAACGGG AAAGATCCTG 
CTACATCACG CAGCAAATTC TGTGAAGCGT GTCTCCATGG AGTTGGGTGG CCTCGCCCCA 
TTCATTGTCT TTGACAGTGC CAACGTAGAC CAGGCCGTCG CGGGGGCAAT GGCGTCTAAA 
TTTAGGAATG CTGGGCAGAC TTGCGTTTGC TCAAACAGAT TCTTGGTACA GCGGGGTATC 
CACGATTCCT TTGTAACGAA ATTTGCGGAA GCCATGAAGA AAAGCCTGCG TGTAGGTAAC 
GGATTTGAGG AAGGAACGAC TCAAGGGCCG TTAATTAATG AGAAAGCTGT AGAAAAGGTA 
GAGAAGCAGG TGAATGACGC AGTTGCCAAA GGGGCCACGG TTGTGACGGG CGGAAAGCGA 
CACCAAAGTG GAGGAAATTT CTTTGAGCCC ACCCTGCTCA GCAATGTCAC CAGGGACATG 
CTCTGCATCA CTGAGGAGAC CTTTGGACCT CTGGCACCAG TCATCAAGTT TGATAAGGAG 
GAAGAGGCCG TCGCCATCGC AAATGCAGCT GAGGTTGGAT TGGCAGGTTA CTTTTACTCT 
CAAGACCCGG CCCAGATCTG GAGAGTGGCG GAGCAGCTGG AGGTCGGCAT GGTCGGTGTG 
AATGAAGGGC TCATCTCTTC AGTGGAATGT CCTTTTGGTG GGGTGAAACA GTCCGGCCTT 
GGGCGCGAGG GGTCCAAGTA TGGCATTGAC GAGTACCTAG AGGTCAAGTA TGTGTGTTAC 
GGAGGCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766120.1
CDS	94..1665
Translation	MATCFLLRSFWAARPALPPPGRFRPEPAGTPRRSYASGPGGLHADLLRGDSFVGGRWLPAPATFPVYDPASGAKLGTVADCGVPEARAAVRAAYDAFNSWKGVSVKERSLLLRKWYDLMIQNKDDLAKIITAESGKPLKEAQGEILYSALFLEWFSEEARRIYGDIIYTSAKDKRGLVLKQPVGVAAIITPWNFPSAMITRKVGAALAAGCTVVVKPAEDTPYSALALAQLANQAGIPAGVYNVIPCSRNKAKEVGEVLCTDPLVSKISFTGSTATGKILLHHAANSVKRVSMELGGLAPFIVFDSANVDQAVAGAMASKFRNAGQTCVCSNRFLVQRGIHDSFVTKFAEAMKKSLRVGNGFEEGTTQGPLINEKAVEKVEKQVNDAVAKGATVVTGGKRHQSGGNFFEPTLLSNVTRDMLCITEETFGPLAPVIKFDKEEEAVAIANAAEVGLAGYFYSQDPAQIWRVAEQLEVGMVGVNEGLISSVECPFGGVKQSGLGREGSKYGIDEYLEVKYVCYGGL

Target ORF information:

RefSeq Version	NM_172532.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aldhehyde dehydrogenase family 5, subfamily A1 (Aldh5a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172532.3

ORF Insert Sequence:

ATGGCGACCT GCTTTCTGCT GCGGAGCTTC TGGGCCGCGC GTCCTGCGCT GCCGCCTCCC 
GGCCGCTTCC GCCCGGAGCC CGCAGGAACC CCGCGGCGCA GCTACGCCAG CGGCCCCGGG 
GGCCTCCACG CCGACCTACT CCGCGGGGAC AGCTTCGTGG GCGGCCGCTG GCTCCCAGCC 
CCCGCCACCT TCCCGGTGTA CGACCCGGCC AGCGGCGCCA AGCTGGGCAC CGTGGCCGAC 
TGCGGGGTAC CCGAGGCCCG CGCTGCTGTG CGCGCCGCCT ACGATGCCTT CAACAGCTGG 
AAAGGGGTCT CGGTCAAGGA GAGGAGCTTA CTACTCCGTA AATGGTACGA CTTAATGATA 
CAAAATAAGG ACGACCTTGC CAAGATCATA ACAGCCGAGA GTGGAAAGCC ACTGAAAGAG 
GCCCAGGGAG AAATTCTGTA TTCAGCCCTT TTCCTGGAGT GGTTCTCAGA GGAAGCTCGC 
CGTATTTATG GAGACATCAT CTATACCTCT GCCAAAGATA AGCGAGGGCT AGTCCTCAAG 
CAGCCCGTTG GTGTGGCGGC CATCATCACA CCGTGGAATT TTCCCAGTGC GATGATCACC 
CGGAAAGTGG GGGCCGCCTT GGCAGCTGGC TGCACTGTGG TGGTGAAACC TGCTGAAGAC 
ACACCCTATT CCGCCCTGGC CCTGGCTCAG CTTGCAAACC AGGCGGGAAT CCCTGCAGGT 
GTCTACAACG TCATTCCCTG CTCTAGAAAC AAGGCCAAGG AAGTGGGAGA GGTCCTGTGT 
ACCGATCCAT TGGTGTCCAA AATTTCCTTT ACTGGCTCAA CAGCAACGGG AAAGATCCTG 
CTACATCACG CAGCAAATTC TGTGAAGCGT GTCTCCATGG AGTTGGGTGG CCTCGCCCCA 
TTCATTGTCT TTGACAGTGC CAACGTAGAC CAGGCCGTCG CGGGGGCAAT GGCGTCTAAA 
TTTAGGAATG CTGGGCAGAC TTGCGTTTGC TCAAACAGAT TCTTGGTACA GCGGGGTATC 
CACGATTCCT TTGTAACGAA ATTTGCGGAA GCCATGAAGA AAAGCCTGCG TGTAGGTAAC 
GGATTTGAGG AAGGAACGAC TCAAGGGCCG TTAATTAATG AGAAAGCTGT AGAAAAGGTA 
GAGAAGCAGG TGAATGACGC AGTTGCCAAA GGGGCCACGG TTGTGACGGG CGGAAAGCGA 
CACCAAAGTG GAGGAAATTT CTTTGAGCCC ACCCTGCTCA GCAATGTCAC CAGGGACATG 
CTCTGCATCA CTGAGGAGAC CTTTGGACCT CTGGCACCAG TCATCAAGTT TGATAAGGAG 
GAAGAGGCCG TCGCCATCGC AAATGCAGCT GAGGTTGGAT TGGCAGGTTA CTTTTACTCT 
CAAGACCCGG CCCAGATCTG GAGAGTGGCG GAGCAGCTGG AGGTCGGCAT GGTCGGTGTG 
AATGAAGGGC TCATCTCTTC AGTGGAATGT CCTTTTGGTG GGGTGAAACA GTCCGGCCTT 
GGGCGCGAGG GGTCCAAGTA TGGCATTGAC GAGTACCTAG AGGTCAAGTA TGTGTGTTAC 
GGAGGCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In vitro modeling of experimental succinic semialdehyde dehydrogenase deficiency (SSADHD) using brain-derived neural stem cells.
PloS one12(10)e0186919(2017)
Vogel KR,Ainslie GR,Jansen EE,Salomons GS,Roullet JB,Gibson KM

Therapeutic relevance of mTOR inhibition in murine succinate semialdehyde dehydrogenase deficiency (SSADHD), a disorder of GABA metabolism.
Biochimica et biophysica acta1863(1)33-42(2017 Jan)
Vogel KR,Ainslie GR,Jansen EE,Salomons GS,Gibson KM

Defects in GABA metabolism affect selective autophagy pathways and are alleviated by mTOR inhibition.
EMBO molecular medicine6(4)551-66(2014 04)
Lakhani R,Vogel KR,Till A,Liu J,Burnett SF,Gibson KM,Subramani S

Aberrant GABA(A) receptor-mediated inhibition in cortico-thalamic networks of succinic semialdehyde dehydrogenase deficient mice.
PloS one6(4)e19021(2011 Apr)
Errington AC,Gibson KM,Crunelli V,Cope DW

Plasticity of postsynaptic, but not presynaptic, GABAB receptors in SSADH deficient mice.
Experimental neurology225(1)114-22(2010 Sep)
Vardya I,Drasbek KR,Gibson KM,Jensen K

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Aldh5a1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報