RNF103 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF103
Entrez Gene ID	7844
Full Name	ring finger protein 103
Synonyms	HKF-1,KF-1,KF1,ZFP-103,ZFP103
General protein information	Preferred Names ring finger protein 103 Names E3 ubiquitin-protein ligase RNF103 RING-type E3 ubiquitin transferase RNF103 zinc finger protein 103 homolog zinc finger protein expressed in cerebellum
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p11.2
Summary	The protein encoded by this gene contains a RING-H2 finger, a motif known to be involved in protein-protein and protein-DNA interactions. This gene is highly expressed in normal cerebellum, but not in the cerebral cortex. The expression of the rat counterpart in the frontal cortex and hippocampus was shown to be induced by elctroconvulsive treatment (ECT) as well as chronic antidepressant treatment, suggesting that this gene may be a molecular target for ECT and antidepressants. The protein is a ubiquitin ligase that functions in the endoplasmic reticulum-associated degradation pathway. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream CHMP3 (charged multivesicular body protein 3) gene. [provided by RefSeq, Oct 2011].
Disorder MIM:	602507

mRNA and Protein(s)

mRNA	Protein	Name
NM_001198952.1	NP_001185881.1	E3 ubiquitin-protein ligase RNF103 isoform 3
NM_001198952.2	NP_001185881.1	E3 ubiquitin-protein ligase RNF103 isoform 3
NM_001198951.1	NP_001185880.1	E3 ubiquitin-protein ligase RNF103 isoform 2
NM_005667.3	NP_005658.1	E3 ubiquitin-protein ligase RNF103 isoform 1
NM_005667.4	NP_005658.1	E3 ubiquitin-protein ligase RNF103 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF103	NP_005658.1
Bos taurus (cattle)	RNF103	NP_001178337.1
	RNF103	XP_001092425.1
Gallus gallus (chicken)	RNF103	NP_001026340.1
Xenopus tropicalis (tropical clawed frog)	rnf103	XP_002937319.1
Pan troglodytes (chimpanzee)	RNF103	XP_515598.3
Mus musculus (house mouse)	Rnf103	NP_033569.2
Rattus norvegicus (Norway rat)	Rnf103	NP_445890.2
Danio rerio (zebrafish)	rnf103	NP_001004600.1
Canis lupus familiaris (dog)	RNF103	XP_532971.2

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes.
Scheper J, Oliva B, Vill?-Freixa J, Thomson TM
Proteins74(1)92-103(2009 Jan)

Ubiquitin ligase Kf-1 is involved in the endoplasmic reticulum-associated degradation pathway.
Maruyama Y, Yamada M, Takahashi K, Yamada M
Biochemical and biophysical research communications374(4)737-41(2008 Oct)

Expression and evaluation of RING finger proteins.
Yang Y, Lorick KL, Jensen JP, Weissman AM
Methods in enzymology398103-12(2005)

Induction of kf-1 after repeated electroconvulsive treatment and chronic antidepressant treatment in rat frontal cortex and hippocampus.
Nishioka G, Yamada M, Kudo K, Takahashi K, Kiuchi Y, Higuchi T, Momose K, Kamijima K, Yamada M
Journal of neural transmission (Vienna, Austria : 1996)110(3)277-85(2003 Mar)

Identification of a novel gene with RING-H2 finger motif induced after chronic antidepressant treatment in rat brain.
Yamada M, Yamada M, Yamazaki S, Takahashi K, Nishioka G, Kudo K, Ozawa H, Yamada S, Kiuchi Y, Kamijima K, Higuchi T, Momose K
Biochemical and biophysical research communications278(1)150-7(2000 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analysis reveals that the main determinants of selectivity between ubiquitin ligases RNF103 and E2 ubiquitin-conjugating enzymes resides on ring domains, rather than on the E2s.
Title: Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes.

Kf-1 is an ER ubiquitin ligase involved in the endoplasmic reticulum-associated degradation pathway.
Title: Ubiquitin ligase Kf-1 is involved in the endoplasmic reticulum-associated degradation pathway.

The following RNF103 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF103 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05055	NM_005667.4 Latest version!	Homo sapiens ring finger protein 103 (RNF103), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu05899	NM_001198952.2 Latest version!	Homo sapiens ring finger protein 103 (RNF103), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu05898	NM_001198951.1 Latest version!	Homo sapiens ring finger protein 103 (RNF103), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$482.30 $689.00
View Old Accession Versions >>
OHu05899	NM_001198952.1	Homo sapiens ring finger protein 103 (RNF103), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu05055	NM_005667.3	Homo sapiens ring finger protein 103 (RNF103), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF103 ( NM_005667.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005658.1 Homo sapiens ring finger protein 103 (RNF103), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05055
Clone ID Related Accession (Same CDS sequence)	NM_005667.3 , NM_005667.4
Accession Version	NM_005667.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-14
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF103 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA816879.1, BC035053.2, AW964704.1 and BC110333.1. On Nov 22, 2018 this sequence version replaced NM_005667.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC035053.2, D76444.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2467147 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000237455.5/ ENSP00000237455.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGCTGA AGCTTTTTTT CTTGCTCCTC TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTATGAAACT GGCATCTTTG CCACCCAGCT GGTGGATCCG 
GTGGCGCTGA GCTTCAAGAA GCTGAAGACC ATTTTGGAGT GCCGGGGGTT GGGCTACTCA 
GGGTTGCCCG AGAAGAAGGA TGTCCGGGAG CTGGTGGAAA AGTCAGGTGA CTTGATGGAG 
GGTGAGCTCT ATTCTGCTCT CAAGGAAGAA GAAGCATCCG AATCGGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCACTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCC TTGGTGGGCA AAATTCACTG GGAGAAAATG 
GTTAAAAAGG TGTCAAGATT TGGAATACGT ACAGGCACAT TTAACTGTTC CAGTGATCCC 
AGATATTGCA GGAGAAGAGG CTGGGTCCGA TCCACACTCA TTATGTCTGT TCCACAAACA 
AGTACTTCAA AAGGGAAAGT CATGCTTAAA GAATACAGTG GACGCAAGAT TGAAGTAGAG 
CACATTTTTA AATGGATAAC TGCTCATGCA GCTTCTCGGA TCAAAACCAT TTATAATGCT 
GAACACTTGA AAGAAGAATG GAATAAAAGT GATCAGTATT GGTTAAAAAT ATACCTATTT 
GCAAACCTTG ACCAGCCCCC AGCTTTCTTC TCTGCACTAA GTATAAAGTT TACTGGAAGA 
GTTGAGTTTA TTTTTGTTAA TGTAGAAAAT TGGGACAACA AGAGTTATAT GACAGATATT 
GGCATATATA ATATGCCATC ATACATACTT AGAACTCCTG AAGGAATTTA CAGGTATGGA 
AACCACACAG GCGAATTTAT ATCCCTTCAG GCCATGGATT CATTTTTGCG CTCATTACAA 
CCCGAGGTAA ATGATCTGTT TGTTTTGAGC TTGGTTCTAG TTAATCTTAT GGCTTGGATG 
GACTTATTTA TTACACAAGG AGCTACCATA AAGCGATTTG TGGTTCTCAT AAGCACTTTA 
GGGACATATA ATTCTCTATT AATTATTTCC TGGCTACCTG TGTTGGGCTT TTTACAGCTA 
CCTTACTTAG ATAGCTTTTA TGAATATAGC TTAAAATTGT TGAGATATTC CAATACAACC 
ACACTGGCTT CATGGGTAAG GGCAGACTGG ATGTTTTACT CTTCACACCC AGCCCTGTTT 
CTCAGTACAT ACCTTGGTCA TGGTTTACTA ATTGATTACT TTGAGAAGAA GAGAAGGCGC 
AACAACAACA ATGATGAAGT CAATGCCAAT AACTTAGAAT GGTTATCAAG TCTGTGGGAC 
TGGTACACCA GCTACCTCTT CCACCCGATT GCTTCTTTTC AGAACTTTCC TGTAGAATCT 
GATTGGGACG AAGACCCTGA CTTATTCTTG GAGCGCTTAG CTTTCCCTGA CCTTTGGCTT 
CACCCTCTGA TACCAACTGA TTATATTAAA AACTTACCAA TGTGGCGATT TAAATGTCTT 
GGAGTCCAGT CTGAAGAGGA AATGTCGGAG GGGTCTCAAG ATACTGAAAA TGACTCGGAA 
AGTGAGAACA CAGACACTTT GAGTAGTGAG AAGGAAGTAT TTGAAGATAA GCAAAGCGTA 
CTTCACAATT CTCCAGGAAC AGCAAGTCAC TGTGATGCTG AGGCTTGTTC ATGTGCCAAT 
AAATATTGTC AGACCAGCCC ATGTGAAAGG AAGGGGAGGT CATATGGATC ATATAACACT 
AATGAAGATA TGGAACCTGA TTGGTTAACT TGGCCTGCTG ATATGCTGCA CTGTACTGAA 
TGTGTTGTTT GCCTAGAGAA TTTTGAAAAT GGATGTTTGC TAATGGGGTT GCCTTGTGGT 
CATGTGTTTC ATCAGAATTG CATTGTGATG TGGTTGGCTG GGGGCCGACA TTGTTGCCCT 
GTTTGCCGGT GGCCTTCTTA TAAAAAAAAG CAGCCATATG CACAACACCA GCCCTTGTCA 
AATGATGTCC CATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005658.1
CDS	980..3037
Translation	MWLKLFFLLLYFLVLFVLARFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRELVEKSGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQVIANDRSPLVGKIHWEKMVKKVSRFGIRTGTFNCSSDPRYCRRRGWVRSTLIMSVPQTSTSKGKVMLKEYSGRKIEVEHIFKWITAHAASRIKTIYNAEHLKEEWNKSDQYWLKIYLFANLDQPPAFFSALSIKFTGRVEFIFVNVENWDNKSYMTDIGIYNMPSYILRTPEGIYRYGNHTGEFISLQAMDSFLRSLQPEVNDLFVLSLVLVNLMAWMDLFITQGATIKRFVVLISTLGTYNSLLIISWLPVLGFLQLPYLDSFYEYSLKLLRYSNTTTLASWVRADWMFYSSHPALFLSTYLGHGLLIDYFEKKRRRNNNNDEVNANNLEWLSSLWDWYTSYLFHPIASFQNFPVESDWDEDPDLFLERLAFPDLWLHPLIPTDYIKNLPMWRFKCLGVQSEEEMSEGSQDTENDSESENTDTLSSEKEVFEDKQSVLHNSPGTASHCDAEACSCANKYCQTSPCERKGRSYGSYNTNEDMEPDWLTWPADMLHCTECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCRWPSYKKKQPYAQHQPLSNDVPS

Target ORF information:

RefSeq Version	NM_005667.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 103 (RNF103), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005667.4

ORF Insert Sequence:

ATGTGGCTGA AGCTTTTTTT CTTGCTCCTC TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTATGAAACT GGCATCTTTG CCACCCAGCT GGTGGATCCG 
GTGGCGCTGA GCTTCAAGAA GCTGAAGACC ATTTTGGAGT GCCGGGGGTT GGGCTACTCA 
GGGTTGCCCG AGAAGAAGGA TGTCCGGGAG CTGGTGGAAA AGTCAGGTGA CTTGATGGAG 
GGTGAGCTCT ATTCTGCTCT CAAGGAAGAA GAAGCATCCG AATCGGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCACTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCC TTGGTGGGCA AAATTCACTG GGAGAAAATG 
GTTAAAAAGG TGTCAAGATT TGGAATACGT ACAGGCACAT TTAACTGTTC CAGTGATCCC 
AGATATTGCA GGAGAAGAGG CTGGGTCCGA TCCACACTCA TTATGTCTGT TCCACAAACA 
AGTACTTCAA AAGGGAAAGT CATGCTTAAA GAATACAGTG GACGCAAGAT TGAAGTAGAG 
CACATTTTTA AATGGATAAC TGCTCATGCA GCTTCTCGGA TCAAAACCAT TTATAATGCT 
GAACACTTGA AAGAAGAATG GAATAAAAGT GATCAGTATT GGTTAAAAAT ATACCTATTT 
GCAAACCTTG ACCAGCCCCC AGCTTTCTTC TCTGCACTAA GTATAAAGTT TACTGGAAGA 
GTTGAGTTTA TTTTTGTTAA TGTAGAAAAT TGGGACAACA AGAGTTATAT GACAGATATT 
GGCATATATA ATATGCCATC ATACATACTT AGAACTCCTG AAGGAATTTA CAGGTATGGA 
AACCACACAG GCGAATTTAT ATCCCTTCAG GCCATGGATT CATTTTTGCG CTCATTACAA 
CCCGAGGTAA ATGATCTGTT TGTTTTGAGC TTGGTTCTAG TTAATCTTAT GGCTTGGATG 
GACTTATTTA TTACACAAGG AGCTACCATA AAGCGATTTG TGGTTCTCAT AAGCACTTTA 
GGGACATATA ATTCTCTATT AATTATTTCC TGGCTACCTG TGTTGGGCTT TTTACAGCTA 
CCTTACTTAG ATAGCTTTTA TGAATATAGC TTAAAATTGT TGAGATATTC CAATACAACC 
ACACTGGCTT CATGGGTAAG GGCAGACTGG ATGTTTTACT CTTCACACCC AGCCCTGTTT 
CTCAGTACAT ACCTTGGTCA TGGTTTACTA ATTGATTACT TTGAGAAGAA GAGAAGGCGC 
AACAACAACA ATGATGAAGT CAATGCCAAT AACTTAGAAT GGTTATCAAG TCTGTGGGAC 
TGGTACACCA GCTACCTCTT CCACCCGATT GCTTCTTTTC AGAACTTTCC TGTAGAATCT 
GATTGGGACG AAGACCCTGA CTTATTCTTG GAGCGCTTAG CTTTCCCTGA CCTTTGGCTT 
CACCCTCTGA TACCAACTGA TTATATTAAA AACTTACCAA TGTGGCGATT TAAATGTCTT 
GGAGTCCAGT CTGAAGAGGA AATGTCGGAG GGGTCTCAAG ATACTGAAAA TGACTCGGAA 
AGTGAGAACA CAGACACTTT GAGTAGTGAG AAGGAAGTAT TTGAAGATAA GCAAAGCGTA 
CTTCACAATT CTCCAGGAAC AGCAAGTCAC TGTGATGCTG AGGCTTGTTC ATGTGCCAAT 
AAATATTGTC AGACCAGCCC ATGTGAAAGG AAGGGGAGGT CATATGGATC ATATAACACT 
AATGAAGATA TGGAACCTGA TTGGTTAACT TGGCCTGCTG ATATGCTGCA CTGTACTGAA 
TGTGTTGTTT GCCTAGAGAA TTTTGAAAAT GGATGTTTGC TAATGGGGTT GCCTTGTGGT 
CATGTGTTTC ATCAGAATTG CATTGTGATG TGGTTGGCTG GGGGCCGACA TTGTTGCCCT 
GTTTGCCGGT GGCCTTCTTA TAAAAAAAAG CAGCCATATG CACAACACCA GCCCTTGTCA 
AATGATGTCC CATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF103 ( NM_001198952.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185881.1 Homo sapiens ring finger protein 103 (RNF103), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu05899
Clone ID Related Accession (Same CDS sequence)	NM_001198952.1 , NM_001198952.2
Accession Version	NM_001198952.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF103 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA816879.1, BC035053.2, AW964704.1, BC022477.1, AI150469.1 and CA312650.1. Transcript Variant: This variant (3) contains alternate 3' exon structure and uses an early stop codon, compared to variant 1. The encoded isoform (3) is shorter at the C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC022477.1, BG715491.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGCTGA AGCTTTTTTT CTTGCTCCTC TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTATGAAACT GGCATCTTTG CCACCCAGCT GGTGGATCCG 
GTGGCGCTGA GCTTCAAGAA GCTGAAGACC ATTTTGGAGT GCCGGGGGTT GGGCTACTCA 
GGGTTGCCCG AGAAGAAGGA TGTCCGGGAG CTGGTGGAAA AGTCAGGTGA CTTGATGGAG 
GGTGAGCTCT ATTCTGCTCT CAAGGAAGAA GAAGCATCCG AATCGGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCACTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185881.1
CDS	992..1360
Translation	MWLKLFFLLLYFLVLFVLARFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRELVEKSGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQ

Target ORF information:

RefSeq Version	NM_001198952.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 103 (RNF103), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198952.1

ORF Insert Sequence:

ATGTGGCTGA AGCTTTTTTT CTTGCTCCTC TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTATGAAACT GGCATCTTTG CCACCCAGCT GGTGGATCCG 
GTGGCGCTGA GCTTCAAGAA GCTGAAGACC ATTTTGGAGT GCCGGGGGTT GGGCTACTCA 
GGGTTGCCCG AGAAGAAGGA TGTCCGGGAG CTGGTGGAAA AGTCAGGTGA CTTGATGGAG 
GGTGAGCTCT ATTCTGCTCT CAAGGAAGAA GAAGCATCCG AATCGGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCACTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF103 ( NM_001198952.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185881.1 Homo sapiens ring finger protein 103 (RNF103), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu05899
Clone ID Related Accession (Same CDS sequence)	NM_001198952.1 , NM_001198952.2
Accession Version	NM_001198952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-14
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF103 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA816879.1, BC035053.2, AW964704.1, BC022477.1, AI150469.1 and CA312650.1. On May 31, 2019 this sequence version replaced NM_001198952.1. Transcript Variant: This variant (3) contains alternate 3' exon structure and uses an early stop codon, compared to variant 1. The encoded isoform (3) is shorter at the C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC022477.1, BG715491.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGCTGA AGCTTTTTTT CTTGCTCCTC TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTATGAAACT GGCATCTTTG CCACCCAGCT GGTGGATCCG 
GTGGCGCTGA GCTTCAAGAA GCTGAAGACC ATTTTGGAGT GCCGGGGGTT GGGCTACTCA 
GGGTTGCCCG AGAAGAAGGA TGTCCGGGAG CTGGTGGAAA AGTCAGGTGA CTTGATGGAG 
GGTGAGCTCT ATTCTGCTCT CAAGGAAGAA GAAGCATCCG AATCGGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCACTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185881.1
CDS	980..1348
Translation	MWLKLFFLLLYFLVLFVLARFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRELVEKSGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQ

Target ORF information:

RefSeq Version	NM_001198952.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 103 (RNF103), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198952.2

ORF Insert Sequence:

ATGTGGCTGA AGCTTTTTTT CTTGCTCCTC TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTATGAAACT GGCATCTTTG CCACCCAGCT GGTGGATCCG 
GTGGCGCTGA GCTTCAAGAA GCTGAAGACC ATTTTGGAGT GCCGGGGGTT GGGCTACTCA 
GGGTTGCCCG AGAAGAAGGA TGTCCGGGAG CTGGTGGAAA AGTCAGGTGA CTTGATGGAG 
GGTGAGCTCT ATTCTGCTCT CAAGGAAGAA GAAGCATCCG AATCGGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCACTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF103 ( NM_005667.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005658.1 Homo sapiens ring finger protein 103 (RNF103), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu05055
Clone ID Related Accession (Same CDS sequence)	NM_005667.3 , NM_005667.4
Accession Version	NM_005667.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF103 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA816879.1, BC035053.2, AW964704.1, BC110333.1 and BQ018198.1. On Nov 16, 2010 this sequence version replaced NM_005667.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC035053.2, D76444.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2467147 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGCTGA AGCTTTTTTT CTTGCTCCTC TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTATGAAACT GGCATCTTTG CCACCCAGCT GGTGGATCCG 
GTGGCGCTGA GCTTCAAGAA GCTGAAGACC ATTTTGGAGT GCCGGGGGTT GGGCTACTCA 
GGGTTGCCCG AGAAGAAGGA TGTCCGGGAG CTGGTGGAAA AGTCAGGTGA CTTGATGGAG 
GGTGAGCTCT ATTCTGCTCT CAAGGAAGAA GAAGCATCCG AATCGGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCACTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCC TTGGTGGGCA AAATTCACTG GGAGAAAATG 
GTTAAAAAGG TGTCAAGATT TGGAATACGT ACAGGCACAT TTAACTGTTC CAGTGATCCC 
AGATATTGCA GGAGAAGAGG CTGGGTCCGA TCCACACTCA TTATGTCTGT TCCACAAACA 
AGTACTTCAA AAGGGAAAGT CATGCTTAAA GAATACAGTG GACGCAAGAT TGAAGTAGAG 
CACATTTTTA AATGGATAAC TGCTCATGCA GCTTCTCGGA TCAAAACCAT TTATAATGCT 
GAACACTTGA AAGAAGAATG GAATAAAAGT GATCAGTATT GGTTAAAAAT ATACCTATTT 
GCAAACCTTG ACCAGCCCCC AGCTTTCTTC TCTGCACTAA GTATAAAGTT TACTGGAAGA 
GTTGAGTTTA TTTTTGTTAA TGTAGAAAAT TGGGACAACA AGAGTTATAT GACAGATATT 
GGCATATATA ATATGCCATC ATACATACTT AGAACTCCTG AAGGAATTTA CAGGTATGGA 
AACCACACAG GCGAATTTAT ATCCCTTCAG GCCATGGATT CATTTTTGCG CTCATTACAA 
CCCGAGGTAA ATGATCTGTT TGTTTTGAGC TTGGTTCTAG TTAATCTTAT GGCTTGGATG 
GACTTATTTA TTACACAAGG AGCTACCATA AAGCGATTTG TGGTTCTCAT AAGCACTTTA 
GGGACATATA ATTCTCTATT AATTATTTCC TGGCTACCTG TGTTGGGCTT TTTACAGCTA 
CCTTACTTAG ATAGCTTTTA TGAATATAGC TTAAAATTGT TGAGATATTC CAATACAACC 
ACACTGGCTT CATGGGTAAG GGCAGACTGG ATGTTTTACT CTTCACACCC AGCCCTGTTT 
CTCAGTACAT ACCTTGGTCA TGGTTTACTA ATTGATTACT TTGAGAAGAA GAGAAGGCGC 
AACAACAACA ATGATGAAGT CAATGCCAAT AACTTAGAAT GGTTATCAAG TCTGTGGGAC 
TGGTACACCA GCTACCTCTT CCACCCGATT GCTTCTTTTC AGAACTTTCC TGTAGAATCT 
GATTGGGACG AAGACCCTGA CTTATTCTTG GAGCGCTTAG CTTTCCCTGA CCTTTGGCTT 
CACCCTCTGA TACCAACTGA TTATATTAAA AACTTACCAA TGTGGCGATT TAAATGTCTT 
GGAGTCCAGT CTGAAGAGGA AATGTCGGAG GGGTCTCAAG ATACTGAAAA TGACTCGGAA 
AGTGAGAACA CAGACACTTT GAGTAGTGAG AAGGAAGTAT TTGAAGATAA GCAAAGCGTA 
CTTCACAATT CTCCAGGAAC AGCAAGTCAC TGTGATGCTG AGGCTTGTTC ATGTGCCAAT 
AAATATTGTC AGACCAGCCC ATGTGAAAGG AAGGGGAGGT CATATGGATC ATATAACACT 
AATGAAGATA TGGAACCTGA TTGGTTAACT TGGCCTGCTG ATATGCTGCA CTGTACTGAA 
TGTGTTGTTT GCCTAGAGAA TTTTGAAAAT GGATGTTTGC TAATGGGGTT GCCTTGTGGT 
CATGTGTTTC ATCAGAATTG CATTGTGATG TGGTTGGCTG GGGGCCGACA TTGTTGCCCT 
GTTTGCCGGT GGCCTTCTTA TAAAAAAAAG CAGCCATATG CACAACACCA GCCCTTGTCA 
AATGATGTCC CATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005658.1
CDS	992..3049
Translation	MWLKLFFLLLYFLVLFVLARFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRELVEKSGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQVIANDRSPLVGKIHWEKMVKKVSRFGIRTGTFNCSSDPRYCRRRGWVRSTLIMSVPQTSTSKGKVMLKEYSGRKIEVEHIFKWITAHAASRIKTIYNAEHLKEEWNKSDQYWLKIYLFANLDQPPAFFSALSIKFTGRVEFIFVNVENWDNKSYMTDIGIYNMPSYILRTPEGIYRYGNHTGEFISLQAMDSFLRSLQPEVNDLFVLSLVLVNLMAWMDLFITQGATIKRFVVLISTLGTYNSLLIISWLPVLGFLQLPYLDSFYEYSLKLLRYSNTTTLASWVRADWMFYSSHPALFLSTYLGHGLLIDYFEKKRRRNNNNDEVNANNLEWLSSLWDWYTSYLFHPIASFQNFPVESDWDEDPDLFLERLAFPDLWLHPLIPTDYIKNLPMWRFKCLGVQSEEEMSEGSQDTENDSESENTDTLSSEKEVFEDKQSVLHNSPGTASHCDAEACSCANKYCQTSPCERKGRSYGSYNTNEDMEPDWLTWPADMLHCTECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCRWPSYKKKQPYAQHQPLSNDVPS

Target ORF information:

RefSeq Version	NM_005667.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 103 (RNF103), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005667.3

ORF Insert Sequence:

ATGTGGCTGA AGCTTTTTTT CTTGCTCCTC TATTTCCTGG TCCTGTTCGT CCTGGCCAGG 
TTTTTTGAGG CCATTGTGTG GTATGAAACT GGCATCTTTG CCACCCAGCT GGTGGATCCG 
GTGGCGCTGA GCTTCAAGAA GCTGAAGACC ATTTTGGAGT GCCGGGGGTT GGGCTACTCA 
GGGTTGCCCG AGAAGAAGGA TGTCCGGGAG CTGGTGGAAA AGTCAGGTGA CTTGATGGAG 
GGTGAGCTCT ATTCTGCTCT CAAGGAAGAA GAAGCATCCG AATCGGTTTC TAGTACCAAT 
TTCAGTGGTG AAATGCACTT CTATGAGCTT GTGGAAGACA CAAAAGATGG CATCTGGCTG 
GTTCAGGTCA TAGCAAATGA CAGAAGTCCC TTGGTGGGCA AAATTCACTG GGAGAAAATG 
GTTAAAAAGG TGTCAAGATT TGGAATACGT ACAGGCACAT TTAACTGTTC CAGTGATCCC 
AGATATTGCA GGAGAAGAGG CTGGGTCCGA TCCACACTCA TTATGTCTGT TCCACAAACA 
AGTACTTCAA AAGGGAAAGT CATGCTTAAA GAATACAGTG GACGCAAGAT TGAAGTAGAG 
CACATTTTTA AATGGATAAC TGCTCATGCA GCTTCTCGGA TCAAAACCAT TTATAATGCT 
GAACACTTGA AAGAAGAATG GAATAAAAGT GATCAGTATT GGTTAAAAAT ATACCTATTT 
GCAAACCTTG ACCAGCCCCC AGCTTTCTTC TCTGCACTAA GTATAAAGTT TACTGGAAGA 
GTTGAGTTTA TTTTTGTTAA TGTAGAAAAT TGGGACAACA AGAGTTATAT GACAGATATT 
GGCATATATA ATATGCCATC ATACATACTT AGAACTCCTG AAGGAATTTA CAGGTATGGA 
AACCACACAG GCGAATTTAT ATCCCTTCAG GCCATGGATT CATTTTTGCG CTCATTACAA 
CCCGAGGTAA ATGATCTGTT TGTTTTGAGC TTGGTTCTAG TTAATCTTAT GGCTTGGATG 
GACTTATTTA TTACACAAGG AGCTACCATA AAGCGATTTG TGGTTCTCAT AAGCACTTTA 
GGGACATATA ATTCTCTATT AATTATTTCC TGGCTACCTG TGTTGGGCTT TTTACAGCTA 
CCTTACTTAG ATAGCTTTTA TGAATATAGC TTAAAATTGT TGAGATATTC CAATACAACC 
ACACTGGCTT CATGGGTAAG GGCAGACTGG ATGTTTTACT CTTCACACCC AGCCCTGTTT 
CTCAGTACAT ACCTTGGTCA TGGTTTACTA ATTGATTACT TTGAGAAGAA GAGAAGGCGC 
AACAACAACA ATGATGAAGT CAATGCCAAT AACTTAGAAT GGTTATCAAG TCTGTGGGAC 
TGGTACACCA GCTACCTCTT CCACCCGATT GCTTCTTTTC AGAACTTTCC TGTAGAATCT 
GATTGGGACG AAGACCCTGA CTTATTCTTG GAGCGCTTAG CTTTCCCTGA CCTTTGGCTT 
CACCCTCTGA TACCAACTGA TTATATTAAA AACTTACCAA TGTGGCGATT TAAATGTCTT 
GGAGTCCAGT CTGAAGAGGA AATGTCGGAG GGGTCTCAAG ATACTGAAAA TGACTCGGAA 
AGTGAGAACA CAGACACTTT GAGTAGTGAG AAGGAAGTAT TTGAAGATAA GCAAAGCGTA 
CTTCACAATT CTCCAGGAAC AGCAAGTCAC TGTGATGCTG AGGCTTGTTC ATGTGCCAAT 
AAATATTGTC AGACCAGCCC ATGTGAAAGG AAGGGGAGGT CATATGGATC ATATAACACT 
AATGAAGATA TGGAACCTGA TTGGTTAACT TGGCCTGCTG ATATGCTGCA CTGTACTGAA 
TGTGTTGTTT GCCTAGAGAA TTTTGAAAAT GGATGTTTGC TAATGGGGTT GCCTTGTGGT 
CATGTGTTTC ATCAGAATTG CATTGTGATG TGGTTGGCTG GGGGCCGACA TTGTTGCCCT 
GTTTGCCGGT GGCCTTCTTA TAAAAAAAAG CAGCCATATG CACAACACCA GCCCTTGTCA 
AATGATGTCC CATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF103 ( NM_001198951.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185880.1 Homo sapiens ring finger protein 103 (RNF103), transcript variant 2, mRNA.

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CloneID	OHu05898
Clone ID Related Accession (Same CDS sequence)	NM_001198951.1
Accession Version	NM_001198951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-14
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF103 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA816879.1, BC110333.1 and BQ018198.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: DA816879.1, SRR1803613.199040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467147 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGGC TCCGGCCGCG GCCGCCGCTG ATACACGGTT GTGGAGGGTT TTTTGAGGCC 
ATTGTGTGGT ATGAAACTGG CATCTTTGCC ACCCAGCTGG TGGATCCGGT GGCGCTGAGC 
TTCAAGAAGC TGAAGACCAT TTTGGAGTGC CGGGGGTTGG GCTACTCAGG GTTGCCCGAG 
AAGAAGGATG TCCGGGAGCT GGTGGAAAAG TCAGGTGACT TGATGGAGGG TGAGCTCTAT 
TCTGCTCTCA AGGAAGAAGA AGCATCCGAA TCGGTTTCTA GTACCAATTT CAGTGGTGAA 
ATGCACTTCT ATGAGCTTGT GGAAGACACA AAAGATGGCA TCTGGCTGGT TCAGGTCATA 
GCAAATGACA GAAGTCCCTT GGTGGGCAAA ATTCACTGGG AGAAAATGGT TAAAAAGGTG 
TCAAGATTTG GAATACGTAC AGGCACATTT AACTGTTCCA GTGATCCCAG ATATTGCAGG 
AGAAGAGGCT GGGTCCGATC CACACTCATT ATGTCTGTTC CACAAACAAG TACTTCAAAA 
GGGAAAGTCA TGCTTAAAGA ATACAGTGGA CGCAAGATTG AAGTAGAGCA CATTTTTAAA 
TGGATAACTG CTCATGCAGC TTCTCGGATC AAAACCATTT ATAATGCTGA ACACTTGAAA 
GAAGAATGGA ATAAAAGTGA TCAGTATTGG TTAAAAATAT ACCTATTTGC AAACCTTGAC 
CAGCCCCCAG CTTTCTTCTC TGCACTAAGT ATAAAGTTTA CTGGAAGAGT TGAGTTTATT 
TTTGTTAATG TAGAAAATTG GGACAACAAG AGTTATATGA CAGATATTGG CATATATAAT 
ATGCCATCAT ACATACTTAG AACTCCTGAA GGAATTTACA GGTATGGAAA CCACACAGGC 
GAATTTATAT CCCTTCAGGC CATGGATTCA TTTTTGCGCT CATTACAACC CGAGGTAAAT 
GATCTGTTTG TTTTGAGCTT GGTTCTAGTT AATCTTATGG CTTGGATGGA CTTATTTATT 
ACACAAGGAG CTACCATAAA GCGATTTGTG GTTCTCATAA GCACTTTAGG GACATATAAT 
TCTCTATTAA TTATTTCCTG GCTACCTGTG TTGGGCTTTT TACAGCTACC TTACTTAGAT 
AGCTTTTATG AATATAGCTT AAAATTGTTG AGATATTCCA ATACAACCAC ACTGGCTTCA 
TGGGTAAGGG CAGACTGGAT GTTTTACTCT TCACACCCAG CCCTGTTTCT CAGTACATAC 
CTTGGTCATG GTTTACTAAT TGATTACTTT GAGAAGAAGA GAAGGCGCAA CAACAACAAT 
GATGAAGTCA ATGCCAATAA CTTAGAATGG TTATCAAGTC TGTGGGACTG GTACACCAGC 
TACCTCTTCC ACCCGATTGC TTCTTTTCAG AACTTTCCTG TAGAATCTGA TTGGGACGAA 
GACCCTGACT TATTCTTGGA GCGCTTAGCT TTCCCTGACC TTTGGCTTCA CCCTCTGATA 
CCAACTGATT ATATTAAAAA CTTACCAATG TGGCGATTTA AATGTCTTGG AGTCCAGTCT 
GAAGAGGAAA TGTCGGAGGG GTCTCAAGAT ACTGAAAATG ACTCGGAAAG TGAGAACACA 
GACACTTTGA GTAGTGAGAA GGAAGTATTT GAAGATAAGC AAAGCGTACT TCACAATTCT 
CCAGGAACAG CAAGTCACTG TGATGCTGAG GCTTGTTCAT GTGCCAATAA ATATTGTCAG 
ACCAGCCCAT GTGAAAGGAA GGGGAGGTCA TATGGATCAT ATAACACTAA TGAAGATATG 
GAACCTGATT GGTTAACTTG GCCTGCTGAT ATGCTGCACT GTACTGAATG TGTTGTTTGC 
CTAGAGAATT TTGAAAATGG ATGTTTGCTA ATGGGGTTGC CTTGTGGTCA TGTGTTTCAT 
CAGAATTGCA TTGTGATGTG GTTGGCTGGG GGCCGACATT GTTGCCCTGT TTGCCGGTGG 
CCTTCTTATA AAAAAAAGCA GCCATATGCA CAACACCAGC CCTTGTCAAA TGATGTCCCA 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185880.1
CDS	16..2061
Translation	MSRLRPRPPLIHGCGGFFEAIVWYETGIFATQLVDPVALSFKKLKTILECRGLGYSGLPEKKDVRELVEKSGDLMEGELYSALKEEEASESVSSTNFSGEMHFYELVEDTKDGIWLVQVIANDRSPLVGKIHWEKMVKKVSRFGIRTGTFNCSSDPRYCRRRGWVRSTLIMSVPQTSTSKGKVMLKEYSGRKIEVEHIFKWITAHAASRIKTIYNAEHLKEEWNKSDQYWLKIYLFANLDQPPAFFSALSIKFTGRVEFIFVNVENWDNKSYMTDIGIYNMPSYILRTPEGIYRYGNHTGEFISLQAMDSFLRSLQPEVNDLFVLSLVLVNLMAWMDLFITQGATIKRFVVLISTLGTYNSLLIISWLPVLGFLQLPYLDSFYEYSLKLLRYSNTTTLASWVRADWMFYSSHPALFLSTYLGHGLLIDYFEKKRRRNNNNDEVNANNLEWLSSLWDWYTSYLFHPIASFQNFPVESDWDEDPDLFLERLAFPDLWLHPLIPTDYIKNLPMWRFKCLGVQSEEEMSEGSQDTENDSESENTDTLSSEKEVFEDKQSVLHNSPGTASHCDAEACSCANKYCQTSPCERKGRSYGSYNTNEDMEPDWLTWPADMLHCTECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCRWPSYKKKQPYAQHQPLSNDVPS

Target ORF information:

RefSeq Version	NM_001198951.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 103 (RNF103), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198951.1

ORF Insert Sequence:

ATGTCTCGGC TCCGGCCGCG GCCGCCGCTG ATACACGGTT GTGGAGGGTT TTTTGAGGCC 
ATTGTGTGGT ATGAAACTGG CATCTTTGCC ACCCAGCTGG TGGATCCGGT GGCGCTGAGC 
TTCAAGAAGC TGAAGACCAT TTTGGAGTGC CGGGGGTTGG GCTACTCAGG GTTGCCCGAG 
AAGAAGGATG TCCGGGAGCT GGTGGAAAAG TCAGGTGACT TGATGGAGGG TGAGCTCTAT 
TCTGCTCTCA AGGAAGAAGA AGCATCCGAA TCGGTTTCTA GTACCAATTT CAGTGGTGAA 
ATGCACTTCT ATGAGCTTGT GGAAGACACA AAAGATGGCA TCTGGCTGGT TCAGGTCATA 
GCAAATGACA GAAGTCCCTT GGTGGGCAAA ATTCACTGGG AGAAAATGGT TAAAAAGGTG 
TCAAGATTTG GAATACGTAC AGGCACATTT AACTGTTCCA GTGATCCCAG ATATTGCAGG 
AGAAGAGGCT GGGTCCGATC CACACTCATT ATGTCTGTTC CACAAACAAG TACTTCAAAA 
GGGAAAGTCA TGCTTAAAGA ATACAGTGGA CGCAAGATTG AAGTAGAGCA CATTTTTAAA 
TGGATAACTG CTCATGCAGC TTCTCGGATC AAAACCATTT ATAATGCTGA ACACTTGAAA 
GAAGAATGGA ATAAAAGTGA TCAGTATTGG TTAAAAATAT ACCTATTTGC AAACCTTGAC 
CAGCCCCCAG CTTTCTTCTC TGCACTAAGT ATAAAGTTTA CTGGAAGAGT TGAGTTTATT 
TTTGTTAATG TAGAAAATTG GGACAACAAG AGTTATATGA CAGATATTGG CATATATAAT 
ATGCCATCAT ACATACTTAG AACTCCTGAA GGAATTTACA GGTATGGAAA CCACACAGGC 
GAATTTATAT CCCTTCAGGC CATGGATTCA TTTTTGCGCT CATTACAACC CGAGGTAAAT 
GATCTGTTTG TTTTGAGCTT GGTTCTAGTT AATCTTATGG CTTGGATGGA CTTATTTATT 
ACACAAGGAG CTACCATAAA GCGATTTGTG GTTCTCATAA GCACTTTAGG GACATATAAT 
TCTCTATTAA TTATTTCCTG GCTACCTGTG TTGGGCTTTT TACAGCTACC TTACTTAGAT 
AGCTTTTATG AATATAGCTT AAAATTGTTG AGATATTCCA ATACAACCAC ACTGGCTTCA 
TGGGTAAGGG CAGACTGGAT GTTTTACTCT TCACACCCAG CCCTGTTTCT CAGTACATAC 
CTTGGTCATG GTTTACTAAT TGATTACTTT GAGAAGAAGA GAAGGCGCAA CAACAACAAT 
GATGAAGTCA ATGCCAATAA CTTAGAATGG TTATCAAGTC TGTGGGACTG GTACACCAGC 
TACCTCTTCC ACCCGATTGC TTCTTTTCAG AACTTTCCTG TAGAATCTGA TTGGGACGAA 
GACCCTGACT TATTCTTGGA GCGCTTAGCT TTCCCTGACC TTTGGCTTCA CCCTCTGATA 
CCAACTGATT ATATTAAAAA CTTACCAATG TGGCGATTTA AATGTCTTGG AGTCCAGTCT 
GAAGAGGAAA TGTCGGAGGG GTCTCAAGAT ACTGAAAATG ACTCGGAAAG TGAGAACACA 
GACACTTTGA GTAGTGAGAA GGAAGTATTT GAAGATAAGC AAAGCGTACT TCACAATTCT 
CCAGGAACAG CAAGTCACTG TGATGCTGAG GCTTGTTCAT GTGCCAATAA ATATTGTCAG 
ACCAGCCCAT GTGAAAGGAA GGGGAGGTCA TATGGATCAT ATAACACTAA TGAAGATATG 
GAACCTGATT GGTTAACTTG GCCTGCTGAT ATGCTGCACT GTACTGAATG TGTTGTTTGC 
CTAGAGAATT TTGAAAATGG ATGTTTGCTA ATGGGGTTGC CTTGTGGTCA TGTGTTTCAT 
CAGAATTGCA TTGTGATGTG GTTGGCTGGG GGCCGACATT GTTGCCCTGT TTGCCGGTGG 
CCTTCTTATA AAAAAAAGCA GCCATATGCA CAACACCAGC CCTTGTCAAA TGATGTCCCA 
TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of electrostatic contributions to the selectivity of interactions between RING-finger domains and ubiquitin-conjugating enzymes.
Proteins74(1)92-103(2009 Jan)
Scheper J,Oliva B,Vill?-Freixa J,Thomson TM

Ubiquitin ligase Kf-1 is involved in the endoplasmic reticulum-associated degradation pathway.
Biochemical and biophysical research communications374(4)737-41(2008 Oct)
Maruyama Y,Yamada M,Takahashi K,Yamada M

Expression and evaluation of RING finger proteins.
Methods in enzymology398103-12(2005)
Yang Y,Lorick KL,Jensen JP,Weissman AM

Induction of kf-1 after repeated electroconvulsive treatment and chronic antidepressant treatment in rat frontal cortex and hippocampus.
Journal of neural transmission (Vienna, Austria : 1996)110(3)277-85(2003 Mar)
Nishioka G,Yamada M,Kudo K,Takahashi K,Kiuchi Y,Higuchi T,Momose K,Kamijima K,Yamada M

Identification of a novel gene with RING-H2 finger motif induced after chronic antidepressant treatment in rat brain.
Biochemical and biophysical research communications278(1)150-7(2000 Nov)
Yamada M,Yamada M,Yamazaki S,Takahashi K,Nishioka G,Kudo K,Ozawa H,Yamada S,Kiuchi Y,Kamijima K,Higuchi T,Momose K

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RNF103 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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