SVIL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SVIL
Entrez Gene ID	6840
Full Name	supervillin
General protein information	Preferred Names supervillin Names supervillin archvillin membrane-associated F-actin binding protein p205 p205/p250
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p11.23
Summary	This gene encodes a bipartite protein with distinct amino- and carboxy-terminal domains. The amino-terminus contains nuclear localization signals and the carboxy-terminus contains numerous consecutive sequences with extensive similarity to proteins in the gelsolin family of actin-binding proteins, which cap, nucleate, and/or sever actin filaments. The gene product is tightly associated with both actin filaments and plasma membranes, suggesting a role as a high-affinity link between the actin cytoskeleton and the membrane. The encoded protein appears to aid in both myosin II assembly during cell spreading and disassembly of focal adhesions. Several transcript variants encoding different isoforms of supervillin have been described. [provided by RefSeq, Apr 2016].
Disorder MIM:	604126

mRNA and Protein(s)

mRNA	Protein	Name
XM_011519637.2	XP_011517939.2	supervillin isoform X13
XM_011519635.1	XP_011517937.1	supervillin isoform X8
XM_005252570.2	XP_005252627.1	supervillin isoform X7
XM_005252573.3	XP_005252630.1	supervillin isoform X7
XM_024448141.1	XP_024303909.1	supervillin isoform X4
NM_003174.3	NP_003165.2	supervillin isoform 1
XM_017016577.1	XP_016872066.1	supervillin isoform X5
XM_017016579.1	XP_016872068.1	supervillin isoform X11
XM_017016575.1	XP_016872064.1	supervillin isoform X1
XM_011519630.2	XP_011517932.2	supervillin isoform X2
XM_017016576.1	XP_016872065.1	supervillin isoform X3
XM_011519631.2	XP_011517933.2	supervillin isoform X4
XM_024448140.1	XP_024303908.1	supervillin isoform X3
XM_011519634.1	XP_011517936.1	supervillin isoform X7
XM_011519635.2	XP_011517937.1	supervillin isoform X7
XM_024448145.1	XP_024303913.1	supervillin isoform X9
XM_024448146.1	XP_024303914.1	supervillin isoform X10
XM_024448149.1	XP_024303917.1	supervillin isoform X13
XM_011519636.2	XP_011517938.2	supervillin isoform X9
XM_017016580.1	XP_016872069.1	supervillin isoform X12
XM_017016581.1	XP_016872070.1	supervillin isoform X15
NM_021738.3	NP_068506.2	supervillin isoform 2
NM_001323599.2	NP_001310528.1	supervillin isoform 3
XM_024448142.1	XP_024303910.1	supervillin isoform X5
XM_024448143.1	XP_024303911.1	supervillin isoform X6
XM_024448147.1	XP_024303915.1	supervillin isoform X11
XM_024448148.1	XP_024303916.1	supervillin isoform X12
XM_017016583.1	XP_016872072.1	supervillin isoform X17
XM_011519633.2	XP_011517935.2	supervillin isoform X7
XM_005252573.2	XP_005252630.1	supervillin isoform X8
XM_017016582.1	XP_016872071.1	supervillin isoform X16
XM_011519632.2	XP_011517934.2	supervillin isoform X6
NM_021738.2	NP_068506.2	supervillin isoform 2
NM_001323600.1	NP_001310529.1	supervillin isoform 4
NM_001323599.1	NP_001310528.1	supervillin isoform 3
XM_005252571.3	XP_005252628.1	supervillin isoform X8
XM_017016578.1	XP_016872067.1	supervillin isoform X10
XM_011519638.2	XP_011517940.2	supervillin isoform X14
XM_024448138.1	XP_024303906.1	supervillin isoform X1
XM_024448139.1	XP_024303907.1	supervillin isoform X2
XM_005252571.4	XP_005252628.1	supervillin isoform X7
XM_024448144.1	XP_024303912.1	supervillin isoform X8

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SVIL	NP_776615.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007181	XP_565017.3
Danio rerio (zebrafish)	svilb	XP_003197996.2
Xenopus tropicalis (tropical clawed frog)	svil	NP_001090765.1
Pan troglodytes (chimpanzee)	SVIL	XP_001136112.1
Canis lupus familiaris (dog)	SVIL	XP_005617106.1
Mus musculus (house mouse)	Svil	NP_694793.1
Rattus norvegicus (Norway rat)	Svil	NP_001101886.2
Gallus gallus (chicken)	SVIL	XP_004939254.1
	SVIL	XP_001083894.2
Danio rerio (zebrafish)	svila	NP_001030338.2
Homo sapiens (human)	SVIL	NP_068506.2
Drosophila melanogaster (fruit fly)	CG33232	NP_995968.1

Gene Ontology
Bibliography

Related articles in PubMed

Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle.
Spinazzola JM, Smith TC, Liu M, Luna EJ, Barton ER
Human molecular genetics24(9)2470-81(2015 May)

A specific LSD1/KDM1A isoform regulates neuronal differentiation through H3K9 demethylation.
Laurent B, Ruitu L, Murn J, Hempel K, Ferrao R, Xiang Y, Liu S, Garcia BA, Wu H, Wu F, Steen H, Shi Y
Molecular cell57(6)957-70(2015 Mar)

Supervillin binding to myosin II and synergism with anillin are required for cytokinesis.
Smith TC, Fridy PC, Li Y, Basil S, Arjun S, Friesen RM, Leszyk J, Chait BT, Rout MP, Luna EJ
Molecular biology of the cell24(23)3603-19(2013 Dec)

The role of PLK1-phosphorylated SVIL in myosin II activation and cytokinetic furrowing.
Hasegawa H, Hyodo T, Asano E, Ito S, Maeda M, Kuribayashi H, Natsume A, Wakabayashi T, Hamaguchi M, Senga T
Journal of cell science126(Pt 16)3627-37(2013 Aug)

Supervillin-mediated suppression of p53 protein enhances cell survival.
Fang Z, Luna EJ
The Journal of biological chemistry288(11)7918-29(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results position archvillin as a mechanically sensitive component of the dystrophin complex and demonstrate that signaling defects caused by loss of gamma-SG occur both at the sarcolemma and in the nucleus.
Title: Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle.

SVIL regulates neuronal maturation by controlling LSD1+8a mediated histone H3K9 demethylation.
Title: A specific LSD1/KDM1A isoform regulates neuronal differentiation through H3K9 demethylation.

Supervillin concentrates activated and total myosin II at the furrow, and simultaneous knockdown of supervillin and anillin additively increases cell division failure.
Title: Supervillin binding to myosin II and synergism with anillin are required for cytokinesis.

An actin/myosin-II-binding protein, supervillin (SVIL), is a substrate of PLK1.
Title: The role of PLK1-phosphorylated SVIL in myosin II activation and cytokinetic furrowing.

adhesion regulatory protein supervillin increases cell survival by decreasing levels of the tumor suppressor protein p53 and downstream target genes
Title: Supervillin-mediated suppression of p53 protein enhances cell survival.

The following SVIL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SVIL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu60830	XM_024448149.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu91612	XM_017016583.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu100647	NM_001323599.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu100703	NM_001323600.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu106557	XM_024448145.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu106558	XM_024448146.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu60826	XM_024448144.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu60827	XM_024448147.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu60828	XM_024448148.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91605	XM_017016578.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91606	XM_017016579.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91607	XM_017016580.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91608	XM_011519637.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91609	XM_011519638.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91610	XM_017016581.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu91611	XM_017016582.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu18368	NM_003174.3 Latest version!	Homo sapiens supervillin (SVIL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu106555	XM_024448138.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu106556	XM_024448140.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu60822	XM_024448139.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu60823	XM_024448141.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu60824	XM_024448142.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu60825	XM_024448143.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu91597	XM_017016575.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu91598	XM_011519630.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu91599	XM_017016576.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu91600	XM_011519631.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu91601	XM_017016577.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu91602	XM_011519632.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu91603	XM_011519633.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu91604	XM_011519636.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu13277	NM_021738.3 Latest version!	Homo sapiens supervillin (SVIL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu43844	XM_011519635.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu43844	XM_005252573.3 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu43844	XM_011519634.1 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu43844	XM_005252570.2 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu43844	XM_005252571.4 Latest version!	Homo sapiens supervillin (SVIL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu43844	XM_011519635.1	Homo sapiens supervillin (SVIL), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu43844	XM_005252573.2	Homo sapiens supervillin (SVIL), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu13277	NM_021738.2	Homo sapiens supervillin (SVIL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu100647	NM_001323599.1	Homo sapiens supervillin (SVIL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu43844	XM_005252571.3	Homo sapiens supervillin (SVIL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SVIL ( XM_024448149.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303917.1 Homo sapiens supervillin (SVIL), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu60830
Clone ID Related Accession (Same CDS sequence)	XM_024448149.1
Accession Version	XM_024448149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGTTT CACTGGAATG GCAATTTGAA 
TTCTCTTCTT TGAGACATAC ATGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303917.1
CDS	201..3770
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKVSLEWQFEFSSLRHTWC

Target ORF information:

RefSeq Version	XM_024448149.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448149.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGTTT CACTGGAATG GCAATTTGAA 
TTCTCTTCTT TGAGACATAC ATGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_017016583.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872072.1 Homo sapiens supervillin (SVIL), transcript variant X21, mRNA.

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CloneID	OHu91612
Clone ID Related Accession (Same CDS sequence)	XM_017016583.1
Accession Version	XM_017016583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CTGAACCTAT CCCCGCTTCG 
TGTTCTGGGG GCACCCACCC TGTAATGGCG AGACTTCCTA GCCCCACTGT AGCTAGGAGC 
GCTGTGCAGC CTGCCAGATT GCAGGCCTCT GCTCACCAAA AGGCCTTAGC CAAGGACCAG 
ACAAATGAGG GCAAAGAGCT TGCTGAGCAA GGAGAACCTG ATTCCTCCAC TCTAAGCTTG 
GCCGAAAAGT TGGCCTTGTT TAACAAATTG TCCCAGCCAG TCTCAAAAGC GATTTCTACC 
CGGAACAGAA TAGACACGAG ACAGAGGAGA ATGAACGCTC GCTATCAAAC TCAGCCAGTC 
ACACTGGGAG AGGTGGAGCA GGTGCAGAGT GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG 
AACACATCAG TGTCTACCGT AGCATCCACG GTTGCTCCAA TGTATGCCGG AGATCTTCGC 
ACAAAGCCAC CTCTTGACCA CAATGCAAGT GCCACTGACT ATAAGTTTTC TTCTTCAATA 
GAAAATTCGG ACTCTCCAGT TAGAAGCATT CTGAAATCGC AAGCTTGGCA GCCTTTGGTA 
GAGGGTAGCG AGAACAAGGG AATGTTGAGA GAATATGGAG AGACAGAAAG CAAGAGAGCT 
TTGACAGGTC GAGACAGTGG GATGGAGAAG TATGGGTCCT TTGAGGAAGC AGAAGCATCC 
TACCCCATCC TGAACCGAGC CAGGGAAGGA GACAGCCATA AGGAATCTAA ATATGCTGTT 
CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC CCTCCCATCA CCCACCTCGG GGATGAACCG 
AAGGAATTTT CCATGGCTAA AATGAATGCA CAAGGAAACT TGGACTTGAG GGACAGGCTG 
CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT GTTATGAAAA GGAAGTTTTC ACTAAGAGCG 
GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG ACGGGGACAG CTGCTGGGAA AACTATTGCT 
CAAACCACAG CCCCCGTGTC CTGGAAGCCC CAGGATTCTT CGGAACAGCC ACAGGAGAAG 
CTCTGCAAGA ATCCATGTGC GATGTTTGCT GCTGGAGAGA TCAAAACGCC GACAGGGGAG 
GGCCTTCTTG ACTCACCCAG CAAAACCATG TCTATTAAAG AAAGATTGGC ACTGTTGAAG 
AAAAGCGGGG AGGAAGATTG GAGAAACAGA CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG 
CCGGCCAGCA GCCTGCACAC CCAGGAAGCA GGGCGGTCCC TCATCAAGAA GGTTTCACTG 
GAATGGCAAT TTGAATTCTC TTCTTTGAGA CATACATGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872072.1
CDS	1241..5065
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKVSLEWQFEFSSLRHTWC

Target ORF information:

RefSeq Version	XM_017016583.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016583.1

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CTGAACCTAT CCCCGCTTCG 
TGTTCTGGGG GCACCCACCC TGTAATGGCG AGACTTCCTA GCCCCACTGT AGCTAGGAGC 
GCTGTGCAGC CTGCCAGATT GCAGGCCTCT GCTCACCAAA AGGCCTTAGC CAAGGACCAG 
ACAAATGAGG GCAAAGAGCT TGCTGAGCAA GGAGAACCTG ATTCCTCCAC TCTAAGCTTG 
GCCGAAAAGT TGGCCTTGTT TAACAAATTG TCCCAGCCAG TCTCAAAAGC GATTTCTACC 
CGGAACAGAA TAGACACGAG ACAGAGGAGA ATGAACGCTC GCTATCAAAC TCAGCCAGTC 
ACACTGGGAG AGGTGGAGCA GGTGCAGAGT GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG 
AACACATCAG TGTCTACCGT AGCATCCACG GTTGCTCCAA TGTATGCCGG AGATCTTCGC 
ACAAAGCCAC CTCTTGACCA CAATGCAAGT GCCACTGACT ATAAGTTTTC TTCTTCAATA 
GAAAATTCGG ACTCTCCAGT TAGAAGCATT CTGAAATCGC AAGCTTGGCA GCCTTTGGTA 
GAGGGTAGCG AGAACAAGGG AATGTTGAGA GAATATGGAG AGACAGAAAG CAAGAGAGCT 
TTGACAGGTC GAGACAGTGG GATGGAGAAG TATGGGTCCT TTGAGGAAGC AGAAGCATCC 
TACCCCATCC TGAACCGAGC CAGGGAAGGA GACAGCCATA AGGAATCTAA ATATGCTGTT 
CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC CCTCCCATCA CCCACCTCGG GGATGAACCG 
AAGGAATTTT CCATGGCTAA AATGAATGCA CAAGGAAACT TGGACTTGAG GGACAGGCTG 
CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT GTTATGAAAA GGAAGTTTTC ACTAAGAGCG 
GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG ACGGGGACAG CTGCTGGGAA AACTATTGCT 
CAAACCACAG CCCCCGTGTC CTGGAAGCCC CAGGATTCTT CGGAACAGCC ACAGGAGAAG 
CTCTGCAAGA ATCCATGTGC GATGTTTGCT GCTGGAGAGA TCAAAACGCC GACAGGGGAG 
GGCCTTCTTG ACTCACCCAG CAAAACCATG TCTATTAAAG AAAGATTGGC ACTGTTGAAG 
AAAAGCGGGG AGGAAGATTG GAGAAACAGA CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG 
CCGGCCAGCA GCCTGCACAC CCAGGAAGCA GGGCGGTCCC TCATCAAGAA GGTTTCACTG 
GAATGGCAAT TTGAATTCTC TTCTTTGAGA CATACATGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( NM_001323599.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310528.1 Homo sapiens supervillin (SVIL), transcript variant 3, mRNA.

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CloneID	OHu100647
Clone ID Related Accession (Same CDS sequence)	NM_001323599.2 , NM_001323599.1
Accession Version	NM_001323599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	supervillin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL159170.14, AL160060.11 and AL158167.15. On Jun 2, 2019 this sequence version replaced NM_001323599.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.58958.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGCA AATCACGGGT GGAGAGGTCG GCTGAAGGAC CTGGCTTGCC CACCGGTGTG 
GAACGGGAGA GAGGGTCCCG GAAACCAAGA CGCTATTTTT CTCCTGGTGA AAGTAGAAAA 
ACTTCCGAGA GATTTAGAAC CCAACCTATA ACTTCAGCAG AACGAAAGGA ATCGGATAGG 
TGCACTTCAC ATTCAGAAAC GCCAACTGTC GATGATGAAG AAAAGGTGGA TGAACGAGCC 
AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT TTCAGGGAGA TGGAAAAATC TTTTGATGAA 
CAAAATGTTC CAAAGCGACG CTCAAGAAAC ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG 
CAGGACAGGT CCCTCACCCA GCCCATCACC ACTGAAGAGG TGGTCATCGC AGCCACTGAA 
CCTATCCCCG CTTCGTGTTC TGGGGGCACC CACCCTGTAA TGGCGAGACT TCCTAGCCCC 
ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC AGATTGCAGG CCTCTGCTCA CCAAAAGGCC 
TTAGCCAAGG ACCAGACAAA TGAGGGCAAA GAGCTTGCTG AGCAAGGAGA ACCTGATTCC 
TCCACTCTAA GCTTGGCCGA AAAGTTGGCC TTGTTTAACA AATTGTCCCA GCCAGTCTCA 
AAAGCGATTT CTACCCGGAA CAGAATAGAC ACGAGACAGA GGAGAATGAA CGCTCGCTAT 
CAAACTCAGC CAGTCACACT GGGAGAGGTG GAGCAGGTGC AGAGTGGAAA GCTCATTCCT 
TTCTCACCTG CCGTGAACAC ATCAGTGTCT ACCGTAGCAT CCACGGTTGC TCCAATGTAT 
GCCGGAGATC TTCGCACAAA GCCACCTCTT GACCACAATG CAAGTGCCAC TGACTATAAG 
TTTTCTTCTT CAATAGAAAA TTCGGACTCT CCAGTTAGAA GCATTCTGAA ATCGCAAGCT 
TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC AAGGGAATGT TGAGAGAATA TGGAGAGACA 
GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC AGTGGGATGG AGAAGTATGG GTCCTTTGAG 
GAAGCAGAAG CATCCTACCC CATCCTGAAC CGAGCCAGGG AAGGAGACAG CCATAAGGAA 
TCTAAATATG CTGTTCCCAG AAGAGGAAGC CTGGAACGGG CGAACCCTCC CATCACCCAC 
CTCGGGGATG AACCGAAGGA ATTTTCCATG GCTAAAATGA ATGCACAAGG AAACTTGGAC 
TTGAGGGACA GGCTGCCCTT TGAAGAGAAG GTGGAGGTGG AGAATGTTAT GAAAAGGAAG 
TTTTCACTAA GAGCGGCAGA GTTCGGGGAG CCCACTTCCG AGCAGACGGG GACAGCTGCT 
GGGAAAACTA TTGCTCAAAC CACAGCCCCC GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA 
CAGCCACAGG AGAAGCTCTG CAAGAATCCA TGTGCGATGT TTGCTGCTGG AGAGATCAAA 
ACGCCGACAG GGGAGGGCCT TCTTGACTCA CCCAGCAAAA CCATGTCTAT TAAAGAAAGA 
TTGGCACTGT TGAAGAAAAG CGGGGAGGAA GATTGGAGAA ACAGACTCAG CAGGAGGCAG 
GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG CACACCCAGG AAGCAGGGCG GTCCCTCATC 
AAGAAGCGGG TCACAGAAAG TCGAGAGAGC CAAATGACGA TTGAGGAGAG GAAGCAGCTC 
ATCACTGTGA GAGAGGAGGC CTGGAAGACG AGAGGCAGAG GAGCGGCCAA CGACTCGACC 
CAGTTCACTG TGGCTGGCAG GATGGTGAAG AAAGGTTTGG CGTCACCTAC TGCCATAACC 
CCAGTAGCCT CACCCATTTG CGGTAAAACA AGAGGCACCA CACCCGTTTC CAAACCCCTG 
GAAGATATCG AAGCCAGACC AGATATGCAG TTAGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGCGGGA TGCACGAAAC GGTGCTCACT 
GTCACCGGCA AATCTGTGAA GGAGGTGATG AAGCCAGATG ATGATGAAAC CTTTGCCAAA 
TTTTACCGCA GCGTGGATTA TAATATGCCA AGAAGTCCTG TGGAGATGGA TGAGGACTTC 
GATGTCATTT TCGATCCTTA TGCACCCAAA TTGACGTCTT CCGTGGCCGA GCACAAGCGG 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC TCCAAAAACC CCCTGAAAAT GCTGGCGGCA 
AGAGAAGATC TCCTTCAGGA ATACACTGAG CAGAGATTAA ACGTTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCTTCC AACTCCAACT TCTCAGAAGT CACCCTGGCG 
GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAACTCTA ACAACAGCGC CGTGCCCTAC AAGAGGCTGA TGCTGTTGCA GATTAAAGGA 
AGAAGACATG TGCAGACCAG GCTGGTGGAA CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCTCC CCACTGCTGC TTCCTGTGGG TAGGAGAGTT TGCAAACGTC 
ATAGAAAAGG CGAAGGCCTC AGAACTTGCA ACTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CTTATATCCA AACCATTGAA GAAGGAATTA ATACACACAC TCATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AATCTGCTGG AGACCCAAAA 
GAAGATGAAC TCTATGAAGC AGCCATAATA GAAACTAACT GCATTTACCG TCTCATGGAT 
GACAAACTTG TTCCTGATGA CGACTACTGG GGGAAAATTC CGAAGTGCTC CCTTCTGCAA 
CCCAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTTT ACGTATGGCA TGGGAAAGAA 
GTCACATTAG CACAACGAAA AATAGCATTT CAGCTGGCAA AGCACTTATG GAATGGAACC 
TTTGACTATG AGAACTGTGA CATCAATCCC CTGGATCCTG GAGAATGCAA TCCGCTTATC 
CCCAGAAAAG GACAGGGGCG GCCCGACTGG GCGATATTTG GGAGACTTAC TGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAGTTTCTG GATTGGACGG AACTGAAGAG ATCGAATGAG 
AAGAACCCCG GGGAACTTGC CCAGCACAAG GAAGACCCCA GGACTGATGT CAAGGCATAC 
GATGTGACAC GGATGGTGTC CATGCCCCAG ACGACAGCAG GCACCATCCT GGACGGAGTG 
AACGTCGGCC GTGGCTATGG CCTGGTGGAA GGACACGACA GGAGGCAGTT TGAGATCACC 
AGCGTTTCCG TGGATGTCTG GCACATCCTG GAATTCGACT ATAGCAGGCT CCCCAAACAA 
AGCATCGGGC AGTTCCATGA GGGGGATGCC TATGTGGTCA AGTGGAAGTT CATGGTGAGC 
ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG CACTCGGTGA GGGCAGCCGG CAAAGAGAAG 
TGCGTCTACT TCTTCTGGCA AGGCCGGCAC TCCACCGTGA GTGAGAAGGG CACGTCGGCG 
CTGATGACGG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG 
GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG GGGGGGATGG TGGTGCACTC GGGGAGGCGG 
GAAGAGGAAG AAGAAAATGT GCAAAGTGAG TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG 
CCCGTGGAAG GGAATTTGCT GGAAGTGGCC TGTCACTGTA GCAGCCTGAG GTCCAGAACT 
TCCATGGTGG TGCTTAACGT CAACAAGGCC CTCATCTACC TGTGGCACGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGAAGGACC GCTGCGAACA AGATCAAGGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTCACAATAC ACGAGTGTGA TGAAGGCTCC 
GAGCCACTCG GATTCTGGGA TGCCTTAGGA AGGAGAGACA GGAAAGCCTA CGATTGCATG 
CTTCAAGATC CTGGAAGTTT TAACTTCGCG CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGGGATTTTG CAGCCACAGA GTTTGTGTAC CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC 
ATGCCCTTCC TGCAGGAAGA TCTGTACAGC GCGCCCCAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACTGGT 
TCCGCCCGCA TCCGCTGGGC CTCCGACCGG AAGAGTGCGA TGGAGACTGT GCTCCAGTAC 
TGCAAAGGAA AAAATCTCAA GAAACCAGCC CCCAAGTCTT ACCTTATCCA CGCTGGTCTG 
GAGCCCCTGA CATTCACCAA TATGTTTCCC AGCTGGGAGC ACAGAGAGGA CATCGCTGAG 
ATCACAGAGA TGGACACGGA AGTTTCCAAT CAGATCACCC TCGTGGAAGA CGTCTTAGCC 
AAGCTCTGTA AAACCATTTA CCCGCTGGCC GACCTCCTGG CCAGGCCACT CCCGGAGGGG 
GTCGATCCTC TGAAGCTTGA GATCTATCTC ACCGACGAAG ACTTCGAGTT TGCACTAGAC 
ATGACGAGGG ATGAATACAA CGCCCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310528.1
CDS	652..6366
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSSKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	NM_001323599.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323599.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGCA AATCACGGGT GGAGAGGTCG GCTGAAGGAC CTGGCTTGCC CACCGGTGTG 
GAACGGGAGA GAGGGTCCCG GAAACCAAGA CGCTATTTTT CTCCTGGTGA AAGTAGAAAA 
ACTTCCGAGA GATTTAGAAC CCAACCTATA ACTTCAGCAG AACGAAAGGA ATCGGATAGG 
TGCACTTCAC ATTCAGAAAC GCCAACTGTC GATGATGAAG AAAAGGTGGA TGAACGAGCC 
AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT TTCAGGGAGA TGGAAAAATC TTTTGATGAA 
CAAAATGTTC CAAAGCGACG CTCAAGAAAC ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG 
CAGGACAGGT CCCTCACCCA GCCCATCACC ACTGAAGAGG TGGTCATCGC AGCCACTGAA 
CCTATCCCCG CTTCGTGTTC TGGGGGCACC CACCCTGTAA TGGCGAGACT TCCTAGCCCC 
ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC AGATTGCAGG CCTCTGCTCA CCAAAAGGCC 
TTAGCCAAGG ACCAGACAAA TGAGGGCAAA GAGCTTGCTG AGCAAGGAGA ACCTGATTCC 
TCCACTCTAA GCTTGGCCGA AAAGTTGGCC TTGTTTAACA AATTGTCCCA GCCAGTCTCA 
AAAGCGATTT CTACCCGGAA CAGAATAGAC ACGAGACAGA GGAGAATGAA CGCTCGCTAT 
CAAACTCAGC CAGTCACACT GGGAGAGGTG GAGCAGGTGC AGAGTGGAAA GCTCATTCCT 
TTCTCACCTG CCGTGAACAC ATCAGTGTCT ACCGTAGCAT CCACGGTTGC TCCAATGTAT 
GCCGGAGATC TTCGCACAAA GCCACCTCTT GACCACAATG CAAGTGCCAC TGACTATAAG 
TTTTCTTCTT CAATAGAAAA TTCGGACTCT CCAGTTAGAA GCATTCTGAA ATCGCAAGCT 
TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC AAGGGAATGT TGAGAGAATA TGGAGAGACA 
GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC AGTGGGATGG AGAAGTATGG GTCCTTTGAG 
GAAGCAGAAG CATCCTACCC CATCCTGAAC CGAGCCAGGG AAGGAGACAG CCATAAGGAA 
TCTAAATATG CTGTTCCCAG AAGAGGAAGC CTGGAACGGG CGAACCCTCC CATCACCCAC 
CTCGGGGATG AACCGAAGGA ATTTTCCATG GCTAAAATGA ATGCACAAGG AAACTTGGAC 
TTGAGGGACA GGCTGCCCTT TGAAGAGAAG GTGGAGGTGG AGAATGTTAT GAAAAGGAAG 
TTTTCACTAA GAGCGGCAGA GTTCGGGGAG CCCACTTCCG AGCAGACGGG GACAGCTGCT 
GGGAAAACTA TTGCTCAAAC CACAGCCCCC GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA 
CAGCCACAGG AGAAGCTCTG CAAGAATCCA TGTGCGATGT TTGCTGCTGG AGAGATCAAA 
ACGCCGACAG GGGAGGGCCT TCTTGACTCA CCCAGCAAAA CCATGTCTAT TAAAGAAAGA 
TTGGCACTGT TGAAGAAAAG CGGGGAGGAA GATTGGAGAA ACAGACTCAG CAGGAGGCAG 
GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG CACACCCAGG AAGCAGGGCG GTCCCTCATC 
AAGAAGCGGG TCACAGAAAG TCGAGAGAGC CAAATGACGA TTGAGGAGAG GAAGCAGCTC 
ATCACTGTGA GAGAGGAGGC CTGGAAGACG AGAGGCAGAG GAGCGGCCAA CGACTCGACC 
CAGTTCACTG TGGCTGGCAG GATGGTGAAG AAAGGTTTGG CGTCACCTAC TGCCATAACC 
CCAGTAGCCT CACCCATTTG CGGTAAAACA AGAGGCACCA CACCCGTTTC CAAACCCCTG 
GAAGATATCG AAGCCAGACC AGATATGCAG TTAGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGCGGGA TGCACGAAAC GGTGCTCACT 
GTCACCGGCA AATCTGTGAA GGAGGTGATG AAGCCAGATG ATGATGAAAC CTTTGCCAAA 
TTTTACCGCA GCGTGGATTA TAATATGCCA AGAAGTCCTG TGGAGATGGA TGAGGACTTC 
GATGTCATTT TCGATCCTTA TGCACCCAAA TTGACGTCTT CCGTGGCCGA GCACAAGCGG 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC TCCAAAAACC CCCTGAAAAT GCTGGCGGCA 
AGAGAAGATC TCCTTCAGGA ATACACTGAG CAGAGATTAA ACGTTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCTTCC AACTCCAACT TCTCAGAAGT CACCCTGGCG 
GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAACTCTA ACAACAGCGC CGTGCCCTAC AAGAGGCTGA TGCTGTTGCA GATTAAAGGA 
AGAAGACATG TGCAGACCAG GCTGGTGGAA CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCTCC CCACTGCTGC TTCCTGTGGG TAGGAGAGTT TGCAAACGTC 
ATAGAAAAGG CGAAGGCCTC AGAACTTGCA ACTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CTTATATCCA AACCATTGAA GAAGGAATTA ATACACACAC TCATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AATCTGCTGG AGACCCAAAA 
GAAGATGAAC TCTATGAAGC AGCCATAATA GAAACTAACT GCATTTACCG TCTCATGGAT 
GACAAACTTG TTCCTGATGA CGACTACTGG GGGAAAATTC CGAAGTGCTC CCTTCTGCAA 
CCCAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTTT ACGTATGGCA TGGGAAAGAA 
GTCACATTAG CACAACGAAA AATAGCATTT CAGCTGGCAA AGCACTTATG GAATGGAACC 
TTTGACTATG AGAACTGTGA CATCAATCCC CTGGATCCTG GAGAATGCAA TCCGCTTATC 
CCCAGAAAAG GACAGGGGCG GCCCGACTGG GCGATATTTG GGAGACTTAC TGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAGTTTCTG GATTGGACGG AACTGAAGAG ATCGAATGAG 
AAGAACCCCG GGGAACTTGC CCAGCACAAG GAAGACCCCA GGACTGATGT CAAGGCATAC 
GATGTGACAC GGATGGTGTC CATGCCCCAG ACGACAGCAG GCACCATCCT GGACGGAGTG 
AACGTCGGCC GTGGCTATGG CCTGGTGGAA GGACACGACA GGAGGCAGTT TGAGATCACC 
AGCGTTTCCG TGGATGTCTG GCACATCCTG GAATTCGACT ATAGCAGGCT CCCCAAACAA 
AGCATCGGGC AGTTCCATGA GGGGGATGCC TATGTGGTCA AGTGGAAGTT CATGGTGAGC 
ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG CACTCGGTGA GGGCAGCCGG CAAAGAGAAG 
TGCGTCTACT TCTTCTGGCA AGGCCGGCAC TCCACCGTGA GTGAGAAGGG CACGTCGGCG 
CTGATGACGG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG 
GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG GGGGGGATGG TGGTGCACTC GGGGAGGCGG 
GAAGAGGAAG AAGAAAATGT GCAAAGTGAG TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG 
CCCGTGGAAG GGAATTTGCT GGAAGTGGCC TGTCACTGTA GCAGCCTGAG GTCCAGAACT 
TCCATGGTGG TGCTTAACGT CAACAAGGCC CTCATCTACC TGTGGCACGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGAAGGACC GCTGCGAACA AGATCAAGGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTCACAATAC ACGAGTGTGA TGAAGGCTCC 
GAGCCACTCG GATTCTGGGA TGCCTTAGGA AGGAGAGACA GGAAAGCCTA CGATTGCATG 
CTTCAAGATC CTGGAAGTTT TAACTTCGCG CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGGGATTTTG CAGCCACAGA GTTTGTGTAC CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC 
ATGCCCTTCC TGCAGGAAGA TCTGTACAGC GCGCCCCAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACTGGT 
TCCGCCCGCA TCCGCTGGGC CTCCGACCGG AAGAGTGCGA TGGAGACTGT GCTCCAGTAC 
TGCAAAGGAA AAAATCTCAA GAAACCAGCC CCCAAGTCTT ACCTTATCCA CGCTGGTCTG 
GAGCCCCTGA CATTCACCAA TATGTTTCCC AGCTGGGAGC ACAGAGAGGA CATCGCTGAG 
ATCACAGAGA TGGACACGGA AGTTTCCAAT CAGATCACCC TCGTGGAAGA CGTCTTAGCC 
AAGCTCTGTA AAACCATTTA CCCGCTGGCC GACCTCCTGG CCAGGCCACT CCCGGAGGGG 
GTCGATCCTC TGAAGCTTGA GATCTATCTC ACCGACGAAG ACTTCGAGTT TGCACTAGAC 
ATGACGAGGG ATGAATACAA CGCCCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( NM_001323599.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310528.1 Homo sapiens supervillin (SVIL), transcript variant 3, mRNA.

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CloneID	OHu100647
Clone ID Related Accession (Same CDS sequence)	NM_001323599.2 , NM_001323599.1
Accession Version	NM_001323599.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	supervillin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL159170.14, AL160060.11 and AL158167.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.58958.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGCA AATCACGGGT GGAGAGGTCG GCTGAAGGAC CTGGCTTGCC CACCGGTGTG 
GAACGGGAGA GAGGGTCCCG GAAACCAAGA CGCTATTTTT CTCCTGGTGA AAGTAGAAAA 
ACTTCCGAGA GATTTAGAAC CCAACCTATA ACTTCAGCAG AACGAAAGGA ATCGGATAGG 
TGCACTTCAC ATTCAGAAAC GCCAACTGTC GATGATGAAG AAAAGGTGGA TGAACGAGCC 
AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT TTCAGGGAGA TGGAAAAATC TTTTGATGAA 
CAAAATGTTC CAAAGCGACG CTCAAGAAAC ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG 
CAGGACAGGT CCCTCACCCA GCCCATCACC ACTGAAGAGG TGGTCATCGC AGCCACTGAA 
CCTATCCCCG CTTCGTGTTC TGGGGGCACC CACCCTGTAA TGGCGAGACT TCCTAGCCCC 
ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC AGATTGCAGG CCTCTGCTCA CCAAAAGGCC 
TTAGCCAAGG ACCAGACAAA TGAGGGCAAA GAGCTTGCTG AGCAAGGAGA ACCTGATTCC 
TCCACTCTAA GCTTGGCCGA AAAGTTGGCC TTGTTTAACA AATTGTCCCA GCCAGTCTCA 
AAAGCGATTT CTACCCGGAA CAGAATAGAC ACGAGACAGA GGAGAATGAA CGCTCGCTAT 
CAAACTCAGC CAGTCACACT GGGAGAGGTG GAGCAGGTGC AGAGTGGAAA GCTCATTCCT 
TTCTCACCTG CCGTGAACAC ATCAGTGTCT ACCGTAGCAT CCACGGTTGC TCCAATGTAT 
GCCGGAGATC TTCGCACAAA GCCACCTCTT GACCACAATG CAAGTGCCAC TGACTATAAG 
TTTTCTTCTT CAATAGAAAA TTCGGACTCT CCAGTTAGAA GCATTCTGAA ATCGCAAGCT 
TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC AAGGGAATGT TGAGAGAATA TGGAGAGACA 
GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC AGTGGGATGG AGAAGTATGG GTCCTTTGAG 
GAAGCAGAAG CATCCTACCC CATCCTGAAC CGAGCCAGGG AAGGAGACAG CCATAAGGAA 
TCTAAATATG CTGTTCCCAG AAGAGGAAGC CTGGAACGGG CGAACCCTCC CATCACCCAC 
CTCGGGGATG AACCGAAGGA ATTTTCCATG GCTAAAATGA ATGCACAAGG AAACTTGGAC 
TTGAGGGACA GGCTGCCCTT TGAAGAGAAG GTGGAGGTGG AGAATGTTAT GAAAAGGAAG 
TTTTCACTAA GAGCGGCAGA GTTCGGGGAG CCCACTTCCG AGCAGACGGG GACAGCTGCT 
GGGAAAACTA TTGCTCAAAC CACAGCCCCC GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA 
CAGCCACAGG AGAAGCTCTG CAAGAATCCA TGTGCGATGT TTGCTGCTGG AGAGATCAAA 
ACGCCGACAG GGGAGGGCCT TCTTGACTCA CCCAGCAAAA CCATGTCTAT TAAAGAAAGA 
TTGGCACTGT TGAAGAAAAG CGGGGAGGAA GATTGGAGAA ACAGACTCAG CAGGAGGCAG 
GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG CACACCCAGG AAGCAGGGCG GTCCCTCATC 
AAGAAGCGGG TCACAGAAAG TCGAGAGAGC CAAATGACGA TTGAGGAGAG GAAGCAGCTC 
ATCACTGTGA GAGAGGAGGC CTGGAAGACG AGAGGCAGAG GAGCGGCCAA CGACTCGACC 
CAGTTCACTG TGGCTGGCAG GATGGTGAAG AAAGGTTTGG CGTCACCTAC TGCCATAACC 
CCAGTAGCCT CACCCATTTG CGGTAAAACA AGAGGCACCA CACCCGTTTC CAAACCCCTG 
GAAGATATCG AAGCCAGACC AGATATGCAG TTAGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGCGGGA TGCACGAAAC GGTGCTCACT 
GTCACCGGCA AATCTGTGAA GGAGGTGATG AAGCCAGATG ATGATGAAAC CTTTGCCAAA 
TTTTACCGCA GCGTGGATTA TAATATGCCA AGAAGTCCTG TGGAGATGGA TGAGGACTTC 
GATGTCATTT TCGATCCTTA TGCACCCAAA TTGACGTCTT CCGTGGCCGA GCACAAGCGG 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC TCCAAAAACC CCCTGAAAAT GCTGGCGGCA 
AGAGAAGATC TCCTTCAGGA ATACACTGAG CAGAGATTAA ACGTTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCTTCC AACTCCAACT TCTCAGAAGT CACCCTGGCG 
GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAACTCTA ACAACAGCGC CGTGCCCTAC AAGAGGCTGA TGCTGTTGCA GATTAAAGGA 
AGAAGACATG TGCAGACCAG GCTGGTGGAA CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCTCC CCACTGCTGC TTCCTGTGGG TAGGAGAGTT TGCAAACGTC 
ATAGAAAAGG CGAAGGCCTC AGAACTTGCA ACTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CTTATATCCA AACCATTGAA GAAGGAATTA ATACACACAC TCATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AATCTGCTGG AGACCCAAAA 
GAAGATGAAC TCTATGAAGC AGCCATAATA GAAACTAACT GCATTTACCG TCTCATGGAT 
GACAAACTTG TTCCTGATGA CGACTACTGG GGGAAAATTC CGAAGTGCTC CCTTCTGCAA 
CCCAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTTT ACGTATGGCA TGGGAAAGAA 
GTCACATTAG CACAACGAAA AATAGCATTT CAGCTGGCAA AGCACTTATG GAATGGAACC 
TTTGACTATG AGAACTGTGA CATCAATCCC CTGGATCCTG GAGAATGCAA TCCGCTTATC 
CCCAGAAAAG GACAGGGGCG GCCCGACTGG GCGATATTTG GGAGACTTAC TGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAGTTTCTG GATTGGACGG AACTGAAGAG ATCGAATGAG 
AAGAACCCCG GGGAACTTGC CCAGCACAAG GAAGACCCCA GGACTGATGT CAAGGCATAC 
GATGTGACAC GGATGGTGTC CATGCCCCAG ACGACAGCAG GCACCATCCT GGACGGAGTG 
AACGTCGGCC GTGGCTATGG CCTGGTGGAA GGACACGACA GGAGGCAGTT TGAGATCACC 
AGCGTTTCCG TGGATGTCTG GCACATCCTG GAATTCGACT ATAGCAGGCT CCCCAAACAA 
AGCATCGGGC AGTTCCATGA GGGGGATGCC TATGTGGTCA AGTGGAAGTT CATGGTGAGC 
ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG CACTCGGTGA GGGCAGCCGG CAAAGAGAAG 
TGCGTCTACT TCTTCTGGCA AGGCCGGCAC TCCACCGTGA GTGAGAAGGG CACGTCGGCG 
CTGATGACGG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG 
GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG GGGGGGATGG TGGTGCACTC GGGGAGGCGG 
GAAGAGGAAG AAGAAAATGT GCAAAGTGAG TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG 
CCCGTGGAAG GGAATTTGCT GGAAGTGGCC TGTCACTGTA GCAGCCTGAG GTCCAGAACT 
TCCATGGTGG TGCTTAACGT CAACAAGGCC CTCATCTACC TGTGGCACGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGAAGGACC GCTGCGAACA AGATCAAGGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTCACAATAC ACGAGTGTGA TGAAGGCTCC 
GAGCCACTCG GATTCTGGGA TGCCTTAGGA AGGAGAGACA GGAAAGCCTA CGATTGCATG 
CTTCAAGATC CTGGAAGTTT TAACTTCGCG CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGGGATTTTG CAGCCACAGA GTTTGTGTAC CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC 
ATGCCCTTCC TGCAGGAAGA TCTGTACAGC GCGCCCCAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACTGGT 
TCCGCCCGCA TCCGCTGGGC CTCCGACCGG AAGAGTGCGA TGGAGACTGT GCTCCAGTAC 
TGCAAAGGAA AAAATCTCAA GAAACCAGCC CCCAAGTCTT ACCTTATCCA CGCTGGTCTG 
GAGCCCCTGA CATTCACCAA TATGTTTCCC AGCTGGGAGC ACAGAGAGGA CATCGCTGAG 
ATCACAGAGA TGGACACGGA AGTTTCCAAT CAGATCACCC TCGTGGAAGA CGTCTTAGCC 
AAGCTCTGTA AAACCATTTA CCCGCTGGCC GACCTCCTGG CCAGGCCACT CCCGGAGGGG 
GTCGATCCTC TGAAGCTTGA GATCTATCTC ACCGACGAAG ACTTCGAGTT TGCACTAGAC 
ATGACGAGGG ATGAATACAA CGCCCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310528.1
CDS	717..6431
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSSKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	NM_001323599.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323599.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGCA AATCACGGGT GGAGAGGTCG GCTGAAGGAC CTGGCTTGCC CACCGGTGTG 
GAACGGGAGA GAGGGTCCCG GAAACCAAGA CGCTATTTTT CTCCTGGTGA AAGTAGAAAA 
ACTTCCGAGA GATTTAGAAC CCAACCTATA ACTTCAGCAG AACGAAAGGA ATCGGATAGG 
TGCACTTCAC ATTCAGAAAC GCCAACTGTC GATGATGAAG AAAAGGTGGA TGAACGAGCC 
AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT TTCAGGGAGA TGGAAAAATC TTTTGATGAA 
CAAAATGTTC CAAAGCGACG CTCAAGAAAC ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG 
CAGGACAGGT CCCTCACCCA GCCCATCACC ACTGAAGAGG TGGTCATCGC AGCCACTGAA 
CCTATCCCCG CTTCGTGTTC TGGGGGCACC CACCCTGTAA TGGCGAGACT TCCTAGCCCC 
ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC AGATTGCAGG CCTCTGCTCA CCAAAAGGCC 
TTAGCCAAGG ACCAGACAAA TGAGGGCAAA GAGCTTGCTG AGCAAGGAGA ACCTGATTCC 
TCCACTCTAA GCTTGGCCGA AAAGTTGGCC TTGTTTAACA AATTGTCCCA GCCAGTCTCA 
AAAGCGATTT CTACCCGGAA CAGAATAGAC ACGAGACAGA GGAGAATGAA CGCTCGCTAT 
CAAACTCAGC CAGTCACACT GGGAGAGGTG GAGCAGGTGC AGAGTGGAAA GCTCATTCCT 
TTCTCACCTG CCGTGAACAC ATCAGTGTCT ACCGTAGCAT CCACGGTTGC TCCAATGTAT 
GCCGGAGATC TTCGCACAAA GCCACCTCTT GACCACAATG CAAGTGCCAC TGACTATAAG 
TTTTCTTCTT CAATAGAAAA TTCGGACTCT CCAGTTAGAA GCATTCTGAA ATCGCAAGCT 
TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC AAGGGAATGT TGAGAGAATA TGGAGAGACA 
GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC AGTGGGATGG AGAAGTATGG GTCCTTTGAG 
GAAGCAGAAG CATCCTACCC CATCCTGAAC CGAGCCAGGG AAGGAGACAG CCATAAGGAA 
TCTAAATATG CTGTTCCCAG AAGAGGAAGC CTGGAACGGG CGAACCCTCC CATCACCCAC 
CTCGGGGATG AACCGAAGGA ATTTTCCATG GCTAAAATGA ATGCACAAGG AAACTTGGAC 
TTGAGGGACA GGCTGCCCTT TGAAGAGAAG GTGGAGGTGG AGAATGTTAT GAAAAGGAAG 
TTTTCACTAA GAGCGGCAGA GTTCGGGGAG CCCACTTCCG AGCAGACGGG GACAGCTGCT 
GGGAAAACTA TTGCTCAAAC CACAGCCCCC GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA 
CAGCCACAGG AGAAGCTCTG CAAGAATCCA TGTGCGATGT TTGCTGCTGG AGAGATCAAA 
ACGCCGACAG GGGAGGGCCT TCTTGACTCA CCCAGCAAAA CCATGTCTAT TAAAGAAAGA 
TTGGCACTGT TGAAGAAAAG CGGGGAGGAA GATTGGAGAA ACAGACTCAG CAGGAGGCAG 
GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG CACACCCAGG AAGCAGGGCG GTCCCTCATC 
AAGAAGCGGG TCACAGAAAG TCGAGAGAGC CAAATGACGA TTGAGGAGAG GAAGCAGCTC 
ATCACTGTGA GAGAGGAGGC CTGGAAGACG AGAGGCAGAG GAGCGGCCAA CGACTCGACC 
CAGTTCACTG TGGCTGGCAG GATGGTGAAG AAAGGTTTGG CGTCACCTAC TGCCATAACC 
CCAGTAGCCT CACCCATTTG CGGTAAAACA AGAGGCACCA CACCCGTTTC CAAACCCCTG 
GAAGATATCG AAGCCAGACC AGATATGCAG TTAGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGCGGGA TGCACGAAAC GGTGCTCACT 
GTCACCGGCA AATCTGTGAA GGAGGTGATG AAGCCAGATG ATGATGAAAC CTTTGCCAAA 
TTTTACCGCA GCGTGGATTA TAATATGCCA AGAAGTCCTG TGGAGATGGA TGAGGACTTC 
GATGTCATTT TCGATCCTTA TGCACCCAAA TTGACGTCTT CCGTGGCCGA GCACAAGCGG 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC TCCAAAAACC CCCTGAAAAT GCTGGCGGCA 
AGAGAAGATC TCCTTCAGGA ATACACTGAG CAGAGATTAA ACGTTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCTTCC AACTCCAACT TCTCAGAAGT CACCCTGGCG 
GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAACTCTA ACAACAGCGC CGTGCCCTAC AAGAGGCTGA TGCTGTTGCA GATTAAAGGA 
AGAAGACATG TGCAGACCAG GCTGGTGGAA CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCTCC CCACTGCTGC TTCCTGTGGG TAGGAGAGTT TGCAAACGTC 
ATAGAAAAGG CGAAGGCCTC AGAACTTGCA ACTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CTTATATCCA AACCATTGAA GAAGGAATTA ATACACACAC TCATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AATCTGCTGG AGACCCAAAA 
GAAGATGAAC TCTATGAAGC AGCCATAATA GAAACTAACT GCATTTACCG TCTCATGGAT 
GACAAACTTG TTCCTGATGA CGACTACTGG GGGAAAATTC CGAAGTGCTC CCTTCTGCAA 
CCCAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTTT ACGTATGGCA TGGGAAAGAA 
GTCACATTAG CACAACGAAA AATAGCATTT CAGCTGGCAA AGCACTTATG GAATGGAACC 
TTTGACTATG AGAACTGTGA CATCAATCCC CTGGATCCTG GAGAATGCAA TCCGCTTATC 
CCCAGAAAAG GACAGGGGCG GCCCGACTGG GCGATATTTG GGAGACTTAC TGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAGTTTCTG GATTGGACGG AACTGAAGAG ATCGAATGAG 
AAGAACCCCG GGGAACTTGC CCAGCACAAG GAAGACCCCA GGACTGATGT CAAGGCATAC 
GATGTGACAC GGATGGTGTC CATGCCCCAG ACGACAGCAG GCACCATCCT GGACGGAGTG 
AACGTCGGCC GTGGCTATGG CCTGGTGGAA GGACACGACA GGAGGCAGTT TGAGATCACC 
AGCGTTTCCG TGGATGTCTG GCACATCCTG GAATTCGACT ATAGCAGGCT CCCCAAACAA 
AGCATCGGGC AGTTCCATGA GGGGGATGCC TATGTGGTCA AGTGGAAGTT CATGGTGAGC 
ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG CACTCGGTGA GGGCAGCCGG CAAAGAGAAG 
TGCGTCTACT TCTTCTGGCA AGGCCGGCAC TCCACCGTGA GTGAGAAGGG CACGTCGGCG 
CTGATGACGG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG 
GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG GGGGGGATGG TGGTGCACTC GGGGAGGCGG 
GAAGAGGAAG AAGAAAATGT GCAAAGTGAG TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG 
CCCGTGGAAG GGAATTTGCT GGAAGTGGCC TGTCACTGTA GCAGCCTGAG GTCCAGAACT 
TCCATGGTGG TGCTTAACGT CAACAAGGCC CTCATCTACC TGTGGCACGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGAAGGACC GCTGCGAACA AGATCAAGGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTCACAATAC ACGAGTGTGA TGAAGGCTCC 
GAGCCACTCG GATTCTGGGA TGCCTTAGGA AGGAGAGACA GGAAAGCCTA CGATTGCATG 
CTTCAAGATC CTGGAAGTTT TAACTTCGCG CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGGGATTTTG CAGCCACAGA GTTTGTGTAC CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC 
ATGCCCTTCC TGCAGGAAGA TCTGTACAGC GCGCCCCAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACTGGT 
TCCGCCCGCA TCCGCTGGGC CTCCGACCGG AAGAGTGCGA TGGAGACTGT GCTCCAGTAC 
TGCAAAGGAA AAAATCTCAA GAAACCAGCC CCCAAGTCTT ACCTTATCCA CGCTGGTCTG 
GAGCCCCTGA CATTCACCAA TATGTTTCCC AGCTGGGAGC ACAGAGAGGA CATCGCTGAG 
ATCACAGAGA TGGACACGGA AGTTTCCAAT CAGATCACCC TCGTGGAAGA CGTCTTAGCC 
AAGCTCTGTA AAACCATTTA CCCGCTGGCC GACCTCCTGG CCAGGCCACT CCCGGAGGGG 
GTCGATCCTC TGAAGCTTGA GATCTATCTC ACCGACGAAG ACTTCGAGTT TGCACTAGAC 
ATGACGAGGG ATGAATACAA CGCCCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( NM_001323600.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310529.1 Homo sapiens supervillin (SVIL), transcript variant 4, mRNA.

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CloneID	OHu100703
Clone ID Related Accession (Same CDS sequence)	NM_001323600.1
Accession Version	NM_001323600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	supervillin isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL159170.14, AL160060.11 and AL158167.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.193697.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGGTG CACTTCACAT 
TCAGAAACGC CAACTGTCGA TGATGAAGAA AAGGTGGATG AACGAGCCAA GCTGAGCGTC 
GCCGCCAAGA GGTTGCTTTT CAGGGAGATG GAAAAATCTT TTGATGAACA AAATGTTCCA 
AAGCGACGCT CAAGAAACAC AGCTGTGGAG CAGAGGCTAC GCCGTCTGCA GGACAGGTCC 
CTCACCCAGC CCATCACCAC TGAAGAGGTG GTCATCGCAG CCACTGAACC TATCCCCGCT 
TCGTGTTCTG GGGGCACCCA CCCTGTAATG GCGAGACTTC CTAGCCCCAC TGTAGCTAGG 
AGCGCTGTGC AGCCTGCCAG ATTGCAGGCC TCTGCTCACC AAAAGGCCTT AGCCAAGGAC 
CAGACAAATG AGGGCAAAGA GCTTGCTGAG CAAGGAGAAC CTGATTCCTC CACTCTAAGC 
TTGGCCGAAA AGTTGGCCTT GTTTAACAAA TTGTCCCAGC CAGTCTCAAA AGCGATTTCT 
ACCCGGAACA GAATAGACAC GAGACAGAGG AGAATGAACG CTCGCTATCA AACTCAGCCA 
GTCACACTGG GAGAGGTGGA GCAGGTGCAG AGTGGAAAGC TCATTCCTTT CTCACCTGCC 
GTGAACACAT CAGTGTCTAC CGTAGCATCC ACGGTTGCTC CAATGTATGC CGGAGATCTT 
CGCACAAAGC CACCTCTTGA CCACAATGCA AGTGCCACTG ACTATAAGTT TTCTTCTTCA 
ATAGAAAATT CGGACTCTCC AGTTAGAAGC ATTCTGAAAT CGCAAGCTTG GCAGCCTTTG 
GTAGAGGGTA GCGAGAACAA GGGAATGTTG AGAGAATATG GAGAGACAGA AAGCAAGAGA 
GCTTTGACAG GTCGAGACAG TGGGATGGAG AAGTATGGGT CCTTTGAGGA AGCAGAAGCA 
TCCTACCCCA TCCTGAACCG AGCCAGGGAA GGAGACAGCC ATAAGGAATC TAAATATGCT 
GTTCCCAGAA GAGGAAGCCT GGAACGGGCG AACCCTCCCA TCACCCACCT CGGGGATGAA 
CCGAAGGAAT TTTCCATGGC TAAAATGAAT GCACAAGGAA ACTTGGACTT GAGGGACAGG 
CTGCCCTTTG AAGAGAAGGT GGAGGTGGAG AATGTTATGA AAAGGAAGTT TTCACTAAGA 
GCGGCAGAGT TCGGGGAGCC CACTTCCGAG CAGACGGGGA CAGCTGCTGG GAAAACTATT 
GCTCAAACCA CAGCCCCCGT GTCCTGGAAG CCCCAGGATT CTTCGGAACA GCCACAGGAG 
AAGCTCTGCA AGAATCCATG TGCGATGTTT GCTGCTGGAG AGATCAAAAC GCCGACAGGG 
GAGGGCCTTC TTGACTCACC CAGCAAAACC ATGTCTATTA AAGAAAGATT GGCACTGTTG 
AAGAAAAGCG GGGAGGAAGA TTGGAGAAAC AGACTCAGCA GGAGGCAGGA GGGCGGCAAG 
GCGCCGGCCA GCAGCCTGCA CACCCAGGAA GCAGGGCGGT CCCTCATCAA GAAGCGGGTC 
ACAGAAAGTC GAGAGAGCCA AATGACGATT GAGGAGAGGA AGCAGCTCAT CACTGTGAGA 
GAGGAGGCCT GGAAGACGAG AGGCAGAGGA GCGGCCAACG ACTCGACCCA GTTCACTGTG 
GCTGGCAGGA TGGTGAAGAA AGGTTTGGCG TCACCTACTG CCATAACCCC AGTAGCCTCA 
CCCATTTGCG GTAAAACAAG AGGCACCACA CCCGTTTCCA AACCCCTGGA AGATATCGAA 
GCCAGACCAG ATATGCAGTT AGAATCGGAC CTGAAGTTGG ACAGGCTGGA AACCTTTCTA 
AGAAGGCTGA ATAACAAAGT TGGCGGGATG CACGAAACGG TGCTCACTGT CACCGGCAAA 
TCTGTGAAGG AGGTGATGAA GCCAGATGAT GATGAAACCT TTGCCAAATT TTACCGCAGC 
GTGGATTATA ATATGCCAAG AAGTCCTGTG GAGATGGATG AGGACTTCGA TGTCATTTTC 
GATCCTTATG CACCCAAATT GACGTCTTCC GTGGCCGAGC ACAAGCGGGC AGTTAGGCCC 
AAGCGCCGGG TTCAGGCCTC CAAAAACCCC CTGAAAATGC TGGCGGCAAG AGAAGATCTC 
CTTCAGGAAT ACACTGAGCA GAGATTAAAC GTTGCCTTCA TGGAGTCAAA GCGGATGAAA 
GTAGAAAAGA TGTCTTCCAA CTCCAACTTC TCAGAAGTCA CCCTGGCGGG TTTAGCCAGT 
AAAGAAAACT TCAGCAACGT CAGCCTGCGG AGCGTCAACC TGACGGAACA GAACTCTAAC 
AACAGCGCCG TGCCCTACAA GAGGCTGATG CTGTTGCAGA TTAAAGGAAG AAGACATGTG 
CAGACCAGGC TGGTGGAACC TCGAGCTTCG GCGCTCAACA GTGGGGACTG CTTCCTCCTG 
CTCTCTCCCC ACTGCTGCTT CCTGTGGGTA GGAGAGTTTG CAAACGTCAT AGAAAAGGCG 
AAGGCCTCAG AACTTGCAAC TTTAATTCAG ACAAAGAGGG AACTTGGTTG TAGAGCTACT 
TATATCCAAA CCATTGAAGA AGGAATTAAT ACACACACTC ATGCAGCCAA AGACTTCTGG 
AAGCTTCTGG GTGGCCAAAC CAGTTACCAA TCTGCTGGAG ACCCAAAAGA AGATGAACTC 
TATGAAGCAG CCATAATAGA AACTAACTGC ATTTACCGTC TCATGGATGA CAAACTTGTT 
CCTGATGACG ACTACTGGGG GAAAATTCCG AAGTGCTCCC TTCTGCAACC CAAAGAGGTA 
CTGGTGTTTG ATTTTGGTAG TGAAGTTTAC GTATGGCATG GGAAAGAAGT CACATTAGCA 
CAACGAAAAA TAGCATTTCA GCTGGCAAAG CACTTATGGA ATGGAACCTT TGACTATGAG 
AACTGTGACA TCAATCCCCT GGATCCTGGA GAATGCAATC CGCTTATCCC CAGAAAAGGA 
CAGGGGCGGC CCGACTGGGC GATATTTGGG AGACTTACTG AACACAATGA GACGATTTTG 
TTCAAAGAGA AGTTTCTGGA TTGGACGGAA CTGAAGAGAT CGAATGAGAA GAACCCCGGG 
GAACTTGCCC AGCACAAGGA AGACCCCAGG ACTGATGTCA AGGCATACGA TGTGACACGG 
ATGGTGTCCA TGCCCCAGAC GACAGCAGGC ACCATCCTGG ACGGAGTGAA CGTCGGCCGT 
GGCTATGGCC TGGTGGAAGG ACACGACAGG AGGCAGTTTG AGATCACCAG CGTTTCCGTG 
GATGTCTGGC ACATCCTGGA ATTCGACTAT AGCAGGCTCC CCAAACAAAG CATCGGGCAG 
TTCCATGAGG GGGATGCCTA TGTGGTCAAG TGGAAGTTCA TGGTGAGCAC GGCAGTGGGA 
AGTCGCCAGA AGGGAGAGCA CTCGGTGAGG GCAGCCGGCA AAGAGAAGTG CGTCTACTTC 
TTCTGGCAAG GCCGGCACTC CACCGTGAGT GAGAAGGGCA CGTCGGCGCT GATGACGGTG 
GAGCTGGACG AGGAAAGGGG GGCCCAGGTC CAGGTTCTCC AGGGAAAGGA GCCCCCCTGT 
TTCCTGCAGT GTTTCCAGGG GGGGATGGTG GTGCACTCGG GGAGGCGGGA AGAGGAAGAA 
GAAAATGTGC AAAGTGAGTG GCGGCTGTAC TGCGTGCGTG GAGAGGTGCC CGTGGAAGGG 
AATTTGCTGG AAGTGGCCTG TCACTGTAGC AGCCTGAGGT CCAGAACTTC CATGGTGGTG 
CTTAACGTCA ACAAGGCCCT CATCTACCTG TGGCACGGAT GCAAAGCCCA GGCCCACACG 
AAGGAGGTCG GAAGGACCGC TGCGAACAAG ATCAAGGAAC AATGTCCCCT GGAAGCAGGA 
CTGCATAGTA GCAGCAAAGT CACAATACAC GAGTGTGATG AAGGCTCCGA GCCACTCGGA 
TTCTGGGATG CCTTAGGAAG GAGAGACAGG AAAGCCTACG ATTGCATGCT TCAAGATCCT 
GGAAGTTTTA ACTTCGCGCC CCGCCTGTTC ATCCTCAGCA GCTCCTCTGG GGATTTTGCA 
GCCACAGAGT TTGTGTACCC TGCCCGAGCC CCCTCTGTGG TCAGTTCCAT GCCCTTCCTG 
CAGGAAGATC TGTACAGCGC GCCCCAGCCA GCACTTTTCC TTGTTGACAA TCACCACGAG 
GTGTACCTCT GGCAAGGCTG GTGGCCCATC GAGAACAAGA TCACTGGTTC CGCCCGCATC 
CGCTGGGCCT CCGACCGGAA GAGTGCGATG GAGACTGTGC TCCAGTACTG CAAAGGAAAA 
AATCTCAAGA AACCAGCCCC CAAGTCTTAC CTTATCCACG CTGGTCTGGA GCCCCTGACA 
TTCACCAATA TGTTTCCCAG CTGGGAGCAC AGAGAGGACA TCGCTGAGAT CACAGAGATG 
GACACGGAAG TTTCCAATCA GATCACCCTC GTGGAAGACG TCTTAGCCAA GCTCTGTAAA 
ACCATTTACC CGCTGGCCGA CCTCCTGGCC AGGCCACTCC CGGAGGGGGT CGATCCTCTG 
AAGCTTGAGA TCTATCTCAC CGACGAAGAC TTCGAGTTTG CACTAGACAT GACGAGGGAT 
GAATACAACG CCCTGCCCGC CTGGAAGCAG GTGAACCTGA AGAAAGCAAA AGGCCTGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310529.1
CDS	570..6032
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	NM_001323600.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323600.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGGTG CACTTCACAT 
TCAGAAACGC CAACTGTCGA TGATGAAGAA AAGGTGGATG AACGAGCCAA GCTGAGCGTC 
GCCGCCAAGA GGTTGCTTTT CAGGGAGATG GAAAAATCTT TTGATGAACA AAATGTTCCA 
AAGCGACGCT CAAGAAACAC AGCTGTGGAG CAGAGGCTAC GCCGTCTGCA GGACAGGTCC 
CTCACCCAGC CCATCACCAC TGAAGAGGTG GTCATCGCAG CCACTGAACC TATCCCCGCT 
TCGTGTTCTG GGGGCACCCA CCCTGTAATG GCGAGACTTC CTAGCCCCAC TGTAGCTAGG 
AGCGCTGTGC AGCCTGCCAG ATTGCAGGCC TCTGCTCACC AAAAGGCCTT AGCCAAGGAC 
CAGACAAATG AGGGCAAAGA GCTTGCTGAG CAAGGAGAAC CTGATTCCTC CACTCTAAGC 
TTGGCCGAAA AGTTGGCCTT GTTTAACAAA TTGTCCCAGC CAGTCTCAAA AGCGATTTCT 
ACCCGGAACA GAATAGACAC GAGACAGAGG AGAATGAACG CTCGCTATCA AACTCAGCCA 
GTCACACTGG GAGAGGTGGA GCAGGTGCAG AGTGGAAAGC TCATTCCTTT CTCACCTGCC 
GTGAACACAT CAGTGTCTAC CGTAGCATCC ACGGTTGCTC CAATGTATGC CGGAGATCTT 
CGCACAAAGC CACCTCTTGA CCACAATGCA AGTGCCACTG ACTATAAGTT TTCTTCTTCA 
ATAGAAAATT CGGACTCTCC AGTTAGAAGC ATTCTGAAAT CGCAAGCTTG GCAGCCTTTG 
GTAGAGGGTA GCGAGAACAA GGGAATGTTG AGAGAATATG GAGAGACAGA AAGCAAGAGA 
GCTTTGACAG GTCGAGACAG TGGGATGGAG AAGTATGGGT CCTTTGAGGA AGCAGAAGCA 
TCCTACCCCA TCCTGAACCG AGCCAGGGAA GGAGACAGCC ATAAGGAATC TAAATATGCT 
GTTCCCAGAA GAGGAAGCCT GGAACGGGCG AACCCTCCCA TCACCCACCT CGGGGATGAA 
CCGAAGGAAT TTTCCATGGC TAAAATGAAT GCACAAGGAA ACTTGGACTT GAGGGACAGG 
CTGCCCTTTG AAGAGAAGGT GGAGGTGGAG AATGTTATGA AAAGGAAGTT TTCACTAAGA 
GCGGCAGAGT TCGGGGAGCC CACTTCCGAG CAGACGGGGA CAGCTGCTGG GAAAACTATT 
GCTCAAACCA CAGCCCCCGT GTCCTGGAAG CCCCAGGATT CTTCGGAACA GCCACAGGAG 
AAGCTCTGCA AGAATCCATG TGCGATGTTT GCTGCTGGAG AGATCAAAAC GCCGACAGGG 
GAGGGCCTTC TTGACTCACC CAGCAAAACC ATGTCTATTA AAGAAAGATT GGCACTGTTG 
AAGAAAAGCG GGGAGGAAGA TTGGAGAAAC AGACTCAGCA GGAGGCAGGA GGGCGGCAAG 
GCGCCGGCCA GCAGCCTGCA CACCCAGGAA GCAGGGCGGT CCCTCATCAA GAAGCGGGTC 
ACAGAAAGTC GAGAGAGCCA AATGACGATT GAGGAGAGGA AGCAGCTCAT CACTGTGAGA 
GAGGAGGCCT GGAAGACGAG AGGCAGAGGA GCGGCCAACG ACTCGACCCA GTTCACTGTG 
GCTGGCAGGA TGGTGAAGAA AGGTTTGGCG TCACCTACTG CCATAACCCC AGTAGCCTCA 
CCCATTTGCG GTAAAACAAG AGGCACCACA CCCGTTTCCA AACCCCTGGA AGATATCGAA 
GCCAGACCAG ATATGCAGTT AGAATCGGAC CTGAAGTTGG ACAGGCTGGA AACCTTTCTA 
AGAAGGCTGA ATAACAAAGT TGGCGGGATG CACGAAACGG TGCTCACTGT CACCGGCAAA 
TCTGTGAAGG AGGTGATGAA GCCAGATGAT GATGAAACCT TTGCCAAATT TTACCGCAGC 
GTGGATTATA ATATGCCAAG AAGTCCTGTG GAGATGGATG AGGACTTCGA TGTCATTTTC 
GATCCTTATG CACCCAAATT GACGTCTTCC GTGGCCGAGC ACAAGCGGGC AGTTAGGCCC 
AAGCGCCGGG TTCAGGCCTC CAAAAACCCC CTGAAAATGC TGGCGGCAAG AGAAGATCTC 
CTTCAGGAAT ACACTGAGCA GAGATTAAAC GTTGCCTTCA TGGAGTCAAA GCGGATGAAA 
GTAGAAAAGA TGTCTTCCAA CTCCAACTTC TCAGAAGTCA CCCTGGCGGG TTTAGCCAGT 
AAAGAAAACT TCAGCAACGT CAGCCTGCGG AGCGTCAACC TGACGGAACA GAACTCTAAC 
AACAGCGCCG TGCCCTACAA GAGGCTGATG CTGTTGCAGA TTAAAGGAAG AAGACATGTG 
CAGACCAGGC TGGTGGAACC TCGAGCTTCG GCGCTCAACA GTGGGGACTG CTTCCTCCTG 
CTCTCTCCCC ACTGCTGCTT CCTGTGGGTA GGAGAGTTTG CAAACGTCAT AGAAAAGGCG 
AAGGCCTCAG AACTTGCAAC TTTAATTCAG ACAAAGAGGG AACTTGGTTG TAGAGCTACT 
TATATCCAAA CCATTGAAGA AGGAATTAAT ACACACACTC ATGCAGCCAA AGACTTCTGG 
AAGCTTCTGG GTGGCCAAAC CAGTTACCAA TCTGCTGGAG ACCCAAAAGA AGATGAACTC 
TATGAAGCAG CCATAATAGA AACTAACTGC ATTTACCGTC TCATGGATGA CAAACTTGTT 
CCTGATGACG ACTACTGGGG GAAAATTCCG AAGTGCTCCC TTCTGCAACC CAAAGAGGTA 
CTGGTGTTTG ATTTTGGTAG TGAAGTTTAC GTATGGCATG GGAAAGAAGT CACATTAGCA 
CAACGAAAAA TAGCATTTCA GCTGGCAAAG CACTTATGGA ATGGAACCTT TGACTATGAG 
AACTGTGACA TCAATCCCCT GGATCCTGGA GAATGCAATC CGCTTATCCC CAGAAAAGGA 
CAGGGGCGGC CCGACTGGGC GATATTTGGG AGACTTACTG AACACAATGA GACGATTTTG 
TTCAAAGAGA AGTTTCTGGA TTGGACGGAA CTGAAGAGAT CGAATGAGAA GAACCCCGGG 
GAACTTGCCC AGCACAAGGA AGACCCCAGG ACTGATGTCA AGGCATACGA TGTGACACGG 
ATGGTGTCCA TGCCCCAGAC GACAGCAGGC ACCATCCTGG ACGGAGTGAA CGTCGGCCGT 
GGCTATGGCC TGGTGGAAGG ACACGACAGG AGGCAGTTTG AGATCACCAG CGTTTCCGTG 
GATGTCTGGC ACATCCTGGA ATTCGACTAT AGCAGGCTCC CCAAACAAAG CATCGGGCAG 
TTCCATGAGG GGGATGCCTA TGTGGTCAAG TGGAAGTTCA TGGTGAGCAC GGCAGTGGGA 
AGTCGCCAGA AGGGAGAGCA CTCGGTGAGG GCAGCCGGCA AAGAGAAGTG CGTCTACTTC 
TTCTGGCAAG GCCGGCACTC CACCGTGAGT GAGAAGGGCA CGTCGGCGCT GATGACGGTG 
GAGCTGGACG AGGAAAGGGG GGCCCAGGTC CAGGTTCTCC AGGGAAAGGA GCCCCCCTGT 
TTCCTGCAGT GTTTCCAGGG GGGGATGGTG GTGCACTCGG GGAGGCGGGA AGAGGAAGAA 
GAAAATGTGC AAAGTGAGTG GCGGCTGTAC TGCGTGCGTG GAGAGGTGCC CGTGGAAGGG 
AATTTGCTGG AAGTGGCCTG TCACTGTAGC AGCCTGAGGT CCAGAACTTC CATGGTGGTG 
CTTAACGTCA ACAAGGCCCT CATCTACCTG TGGCACGGAT GCAAAGCCCA GGCCCACACG 
AAGGAGGTCG GAAGGACCGC TGCGAACAAG ATCAAGGAAC AATGTCCCCT GGAAGCAGGA 
CTGCATAGTA GCAGCAAAGT CACAATACAC GAGTGTGATG AAGGCTCCGA GCCACTCGGA 
TTCTGGGATG CCTTAGGAAG GAGAGACAGG AAAGCCTACG ATTGCATGCT TCAAGATCCT 
GGAAGTTTTA ACTTCGCGCC CCGCCTGTTC ATCCTCAGCA GCTCCTCTGG GGATTTTGCA 
GCCACAGAGT TTGTGTACCC TGCCCGAGCC CCCTCTGTGG TCAGTTCCAT GCCCTTCCTG 
CAGGAAGATC TGTACAGCGC GCCCCAGCCA GCACTTTTCC TTGTTGACAA TCACCACGAG 
GTGTACCTCT GGCAAGGCTG GTGGCCCATC GAGAACAAGA TCACTGGTTC CGCCCGCATC 
CGCTGGGCCT CCGACCGGAA GAGTGCGATG GAGACTGTGC TCCAGTACTG CAAAGGAAAA 
AATCTCAAGA AACCAGCCCC CAAGTCTTAC CTTATCCACG CTGGTCTGGA GCCCCTGACA 
TTCACCAATA TGTTTCCCAG CTGGGAGCAC AGAGAGGACA TCGCTGAGAT CACAGAGATG 
GACACGGAAG TTTCCAATCA GATCACCCTC GTGGAAGACG TCTTAGCCAA GCTCTGTAAA 
ACCATTTACC CGCTGGCCGA CCTCCTGGCC AGGCCACTCC CGGAGGGGGT CGATCCTCTG 
AAGCTTGAGA TCTATCTCAC CGACGAAGAC TTCGAGTTTG CACTAGACAT GACGAGGGAT 
GAATACAACG CCCTGCCCGC CTGGAAGCAG GTGAACCTGA AGAAAGCAAA AGGCCTGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448145.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303913.1 Homo sapiens supervillin (SVIL), transcript variant X13, mRNA.

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CloneID	OHu106557
Clone ID Related Accession (Same CDS sequence)	XM_024448145.1
Accession Version	XM_024448145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGCA AATCACGGGT GGAGAGGTCG GCTGAAGGAC CTGGCTTGCC CACCGGTGTG 
GAACGGGAGA GAGGGTCCCG GAAACCAAGA CGCTATTTTT CTCCTGGTGA AAGTAGAAAA 
ACTTCCGAGA GATTTAGAAC CCAACCTATA ACTTCAGCAG AACGAAAGGA ATCGGATAGG 
TGCACTTCAC ATTCAGAAAC GCCAACTGTC GATGATGAAG AAAAGGTGGA TGAACGAGCC 
AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT TTCAGGGAGA TGGAAAAATC TTTTGATGAA 
CAAAATGTTC CAAAGCGACG CTCAAGAAAC ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG 
CAGGACAGGT CCCTCACCCA GCCCATCACC ACTGAAGAGG TGGTCATCGC AGCCACATTG 
CAGGCCTCTG CTCACCAAAA GGCCTTAGCC AAGGACCAGA CAAATGAGGG CAAAGAGCTT 
GCTGAGCAAG GAGAACCTGA TTCCTCCACT CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT 
AACAAATTGT CCCAGCCAGT CTCAAAAGCG ATTTCTACCC GGAACAGAAT AGACACGAGA 
CAGAGGAGAA TGAACGCTCG CTATCAAACT CAGCCAGTCA CACTGGGAGA GGTGGAGCAG 
GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA CCTGCCGTGA ACACATCAGT GTCTACCGTA 
GCATCCACGG TTGCTCCAAT GTATGCCGGA GATCTTCGCA CAAAGCCACC TCTTGACCAC 
AATGCAAGTG CCACTGACTA TAAGTTTTCT TCTTCAATAG AAAATTCGGA CTCTCCAGTT 
AGAAGCATTC TGAAATCGCA AGCTTGGCAG CCTTTGGTAG AGGGTAGCGA GAACAAGGGA 
ATGTTGAGAG AATATGGAGA GACAGAAAGC AAGAGAGCTT TGACAGGTCG AGACAGTGGG 
ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA GAAGCATCCT ACCCCATCCT GAACCGAGCC 
AGGGAAGGAG ACAGCCATAA GGAATCTAAA TATGCTGTTC CCAGAAGAGG AAGCCTGGAA 
CGGGCGAACC CTCCCATCAC CCACCTCGGG GATGAACCGA AGGAATTTTC CATGGCTAAA 
ATGAATGCAC AAGGAAACTT GGACTTGAGG GACAGGCTGC CCTTTGAAGA GAAGGTGGAG 
GTGGAGAATG TTATGAAAAG GAAGTTTTCA CTAAGAGCGG CAGAGTTCGG GGAGCCCACT 
TCCGAGCAGA CGGGGACAGC TGCTGGGAAA ACTATTGCTC AAACCACAGC CCCCGTGTCC 
TGGAAGCCCC AGGATTCTTC GGAACAGCCA CAGGAGAAGC TCTGCAAGAA TCCATGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAACGCCG ACAGGGGAGG GCCTTCTTGA CTCACCCAGC 
AAAACCATGT CTATTAAAGA AAGATTGGCA CTGTTGAAGA AAAGCGGGGA GGAAGATTGG 
AGAAACAGAC TCAGCAGGAG GCAGGAGGGC GGCAAGGCGC CGGCCAGCAG CCTGCACACC 
CAGGAAGCAG GGCGGTCCCT CATCAAGAAG GAAGAAGGAG GAGTTGCGGA TGATAGTGCC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAT GCTTCTACTT ATTCTCCTGC TATTCCTGCT 
GCCCATAAAT ACCTGTCTTT TGTGTCGATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303913.1
CDS	201..5945
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSSKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448145.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448145.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGCA AATCACGGGT GGAGAGGTCG GCTGAAGGAC CTGGCTTGCC CACCGGTGTG 
GAACGGGAGA GAGGGTCCCG GAAACCAAGA CGCTATTTTT CTCCTGGTGA AAGTAGAAAA 
ACTTCCGAGA GATTTAGAAC CCAACCTATA ACTTCAGCAG AACGAAAGGA ATCGGATAGG 
TGCACTTCAC ATTCAGAAAC GCCAACTGTC GATGATGAAG AAAAGGTGGA TGAACGAGCC 
AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT TTCAGGGAGA TGGAAAAATC TTTTGATGAA 
CAAAATGTTC CAAAGCGACG CTCAAGAAAC ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG 
CAGGACAGGT CCCTCACCCA GCCCATCACC ACTGAAGAGG TGGTCATCGC AGCCACATTG 
CAGGCCTCTG CTCACCAAAA GGCCTTAGCC AAGGACCAGA CAAATGAGGG CAAAGAGCTT 
GCTGAGCAAG GAGAACCTGA TTCCTCCACT CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT 
AACAAATTGT CCCAGCCAGT CTCAAAAGCG ATTTCTACCC GGAACAGAAT AGACACGAGA 
CAGAGGAGAA TGAACGCTCG CTATCAAACT CAGCCAGTCA CACTGGGAGA GGTGGAGCAG 
GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA CCTGCCGTGA ACACATCAGT GTCTACCGTA 
GCATCCACGG TTGCTCCAAT GTATGCCGGA GATCTTCGCA CAAAGCCACC TCTTGACCAC 
AATGCAAGTG CCACTGACTA TAAGTTTTCT TCTTCAATAG AAAATTCGGA CTCTCCAGTT 
AGAAGCATTC TGAAATCGCA AGCTTGGCAG CCTTTGGTAG AGGGTAGCGA GAACAAGGGA 
ATGTTGAGAG AATATGGAGA GACAGAAAGC AAGAGAGCTT TGACAGGTCG AGACAGTGGG 
ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA GAAGCATCCT ACCCCATCCT GAACCGAGCC 
AGGGAAGGAG ACAGCCATAA GGAATCTAAA TATGCTGTTC CCAGAAGAGG AAGCCTGGAA 
CGGGCGAACC CTCCCATCAC CCACCTCGGG GATGAACCGA AGGAATTTTC CATGGCTAAA 
ATGAATGCAC AAGGAAACTT GGACTTGAGG GACAGGCTGC CCTTTGAAGA GAAGGTGGAG 
GTGGAGAATG TTATGAAAAG GAAGTTTTCA CTAAGAGCGG CAGAGTTCGG GGAGCCCACT 
TCCGAGCAGA CGGGGACAGC TGCTGGGAAA ACTATTGCTC AAACCACAGC CCCCGTGTCC 
TGGAAGCCCC AGGATTCTTC GGAACAGCCA CAGGAGAAGC TCTGCAAGAA TCCATGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAACGCCG ACAGGGGAGG GCCTTCTTGA CTCACCCAGC 
AAAACCATGT CTATTAAAGA AAGATTGGCA CTGTTGAAGA AAAGCGGGGA GGAAGATTGG 
AGAAACAGAC TCAGCAGGAG GCAGGAGGGC GGCAAGGCGC CGGCCAGCAG CCTGCACACC 
CAGGAAGCAG GGCGGTCCCT CATCAAGAAG GAAGAAGGAG GAGTTGCGGA TGATAGTGCC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAT GCTTCTACTT ATTCTCCTGC TATTCCTGCT 
GCCCATAAAT ACCTGTCTTT TGTGTCGATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448146.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303914.1 Homo sapiens supervillin (SVIL), transcript variant X14, mRNA.

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CloneID	OHu106558
Clone ID Related Accession (Same CDS sequence)	XM_024448146.1
Accession Version	XM_024448146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGGT GCACTTCACA TTCAGAAACG CCAACTGTCG ATGATGAAGA AAAGGTGGAT 
GAACGAGCCA AGCTGAGCGT CGCCGCCAAG AGGTTGCTTT TCAGGGAGAT GGAAAAATCT 
TTTGATGAAC AAAATGTTCC AAAGCGACGC TCAAGAAACA CAGCTGTGGA GCAGAGGCTA 
CGCCGTCTGC AGGACAGGTC CCTCACCCAG CCCATCACCA CTGAAGAGGT GGTCATCGCA 
GCCACTGAAC CTATCCCCGC TTCGTGTTCT GGGGGCACCC ACCCTGTAAT GGCGAGACTT 
CCTAGCCCCA CTGTAGCTAG GAGCGCTGTG CAGCCTGCCA GATTGCAGGC CTCTGCTCAC 
CAAAAGGCCT TAGCCAAGGA CCAGACAAAT GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA 
CCTGATTCCT CCACTCTAAG CTTGGCCGAA AAGTTGGCCT TGTTTAACAA ATTGTCCCAG 
CCAGTCTCAA AAGCGATTTC TACCCGGAAC AGAATAGACA CGAGACAGAG GAGAATGAAC 
GCTCGCTATC AAACTCAGCC AGTCACACTG GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG 
CTCATTCCTT TCTCACCTGC CGTGAACACA TCAGTGTCTA CCGTAGCATC CACGGTTGCT 
CCAATGTATG CCGGAGATCT TCGCACAAAG CCACCTCTTG ACCACAATGC AAGTGCCACT 
GACTATAAGT TTTCTTCTTC AATAGAAAAT TCGGACTCTC CAGTTAGAAG CATTCTGAAA 
TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT AGCGAGAACA AGGGAATGTT GAGAGAATAT 
GGAGAGACAG AAAGCAAGAG AGCTTTGACA GGTCGAGACA GTGGGATGGA GAAGTATGGG 
TCCTTTGAGG AAGCAGAAGC ATCCTACCCC ATCCTGAACC GAGCCAGGGA AGGAGACAGC 
CATAAGGAAT CTAAATATGC TGTTCCCAGA AGAGGAAGCC TGGAACGGGC GAACCCTCCC 
ATCACCCACC TCGGGGATGA ACCGAAGGAA TTTTCCATGG CTAAAATGAA TGCACAAGGA 
AACTTGGACT TGAGGGACAG GCTGCCCTTT GAAGAGAAGG TGGAGGTGGA GAATGTTATG 
AAAAGGAAGT TTTCACTAAG AGCGGCAGAG TTCGGGGAGC CCACTTCCGA GCAGACGGGG 
ACAGCTGCTG GGAAAACTAT TGCTCAAACC ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT 
TCTTCGGAAC AGCCACAGGA GAAGCTCTGC AAGAATCCAT GTGCGATGTT TGCTGCTGGA 
GAGATCAAAA CGCCGACAGG GGAGGGCCTT CTTGACTCAC CCAGCAAAAC CATGTCTATT 
AAAGAAAGAT TGGCACTGTT GAAGAAAAGC GGGGAGGAAG ATTGGAGAAA CAGACTCAGC 
AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC AGCAGCCTGC ACACCCAGGA AGCAGGGCGG 
TCCCTCATCA AGAAGGAAGA AGGAGGAGTT GCGGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATTC CTGCTGCCCA TAAATACCTG 
TCTTTTGTGT CGATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAAAT GACGATTGAG 
GAGAGGAAGC AGCTCATCAC TGTGAGAGAG GAGGCCTGGA AGACGAGAGG CAGAGGAGCG 
GCCAACGACT CGACCCAGTT CACTGTGGCT GGCAGGATGG TGAAGAAAGG TTTGGCGTCA 
CCTACTGCCA TAACCCCAGT AGCCTCACCC ATTTGCGGTA AAACAAGAGG CACCACACCC 
GTTTCCAAAC CCCTGGAAGA TATCGAAGCC AGACCAGATA TGCAGTTAGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG CGGGATGCAC 
GAAACGGTGC TCACTGTCAC CGGCAAATCT GTGAAGGAGG TGATGAAGCC AGATGATGAT 
GAAACCTTTG CCAAATTTTA CCGCAGCGTG GATTATAATA TGCCAAGAAG TCCTGTGGAG 
ATGGATGAGG ACTTCGATGT CATTTTCGAT CCTTATGCAC CCAAATTGAC GTCTTCCGTG 
GCCGAGCACA AGCGGGCAGT TAGGCCCAAG CGCCGGGTTC AGGCCTCCAA AAACCCCCTG 
AAAATGCTGG CGGCAAGAGA AGATCTCCTT CAGGAATACA CTGAGCAGAG ATTAAACGTT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CTTCCAACTC CAACTTCTCA 
GAAGTCACCC TGGCGGGTTT AGCCAGTAAA GAAAACTTCA GCAACGTCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA CTCTAACAAC AGCGCCGTGC CCTACAAGAG GCTGATGCTG 
TTGCAGATTA AAGGAAGAAG ACATGTGCAG ACCAGGCTGG TGGAACCTCG AGCTTCGGCG 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCTCCCCACT GCTGCTTCCT GTGGGTAGGA 
GAGTTTGCAA ACGTCATAGA AAAGGCGAAG GCCTCAGAAC TTGCAACTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACTTAT ATCCAAACCA TTGAAGAAGG AATTAATACA 
CACACTCATG CAGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAATCT 
GCTGGAGACC CAAAAGAAGA TGAACTCTAT GAAGCAGCCA TAATAGAAAC TAACTGCATT 
TACCGTCTCA TGGATGACAA ACTTGTTCCT GATGACGACT ACTGGGGGAA AATTCCGAAG 
TGCTCCCTTC TGCAACCCAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTTTACGTA 
TGGCATGGGA AAGAAGTCAC ATTAGCACAA CGAAAAATAG CATTTCAGCT GGCAAAGCAC 
TTATGGAATG GAACCTTTGA CTATGAGAAC TGTGACATCA ATCCCCTGGA TCCTGGAGAA 
TGCAATCCGC TTATCCCCAG AAAAGGACAG GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA 
CTTACTGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAGT TTCTGGATTG GACGGAACTG 
AAGAGATCGA ATGAGAAGAA CCCCGGGGAA CTTGCCCAGC ACAAGGAAGA CCCCAGGACT 
GATGTCAAGG CATACGATGT GACACGGATG GTGTCCATGC CCCAGACGAC AGCAGGCACC 
ATCCTGGACG GAGTGAACGT CGGCCGTGGC TATGGCCTGG TGGAAGGACA CGACAGGAGG 
CAGTTTGAGA TCACCAGCGT TTCCGTGGAT GTCTGGCACA TCCTGGAATT CGACTATAGC 
AGGCTCCCCA AACAAAGCAT CGGGCAGTTC CATGAGGGGG ATGCCTATGT GGTCAAGTGG 
AAGTTCATGG TGAGCACGGC AGTGGGAAGT CGCCAGAAGG GAGAGCACTC GGTGAGGGCA 
GCCGGCAAAG AGAAGTGCGT CTACTTCTTC TGGCAAGGCC GGCACTCCAC CGTGAGTGAG 
AAGGGCACGT CGGCGCTGAT GACGGTGGAG CTGGACGAGG AAAGGGGGGC CCAGGTCCAG 
GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC CTGCAGTGTT TCCAGGGGGG GATGGTGGTG 
CACTCGGGGA GGCGGGAAGA GGAAGAAGAA AATGTGCAAA GTGAGTGGCG GCTGTACTGC 
GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC 
CTGAGGTCCA GAACTTCCAT GGTGGTGCTT AACGTCAACA AGGCCCTCAT CTACCTGTGG 
CACGGATGCA AAGCCCAGGC CCACACGAAG GAGGTCGGAA GGACCGCTGC GAACAAGATC 
AAGGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTCAC AATACACGAG 
TGTGATGAAG GCTCCGAGCC ACTCGGATTC TGGGATGCCT TAGGAAGGAG AGACAGGAAA 
GCCTACGATT GCATGCTTCA AGATCCTGGA AGTTTTAACT TCGCGCCCCG CCTGTTCATC 
CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC ACAGAGTTTG TGTACCCTGC CCGAGCCCCC 
TCTGTGGTCA GTTCCATGCC CTTCCTGCAG GAAGATCTGT ACAGCGCGCC CCAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTG TACCTCTGGC AAGGCTGGTG GCCCATCGAG 
AACAAGATCA CTGGTTCCGC CCGCATCCGC TGGGCCTCCG ACCGGAAGAG TGCGATGGAG 
ACTGTGCTCC AGTACTGCAA AGGAAAAAAT CTCAAGAAAC CAGCCCCCAA GTCTTACCTT 
ATCCACGCTG GTCTGGAGCC CCTGACATTC ACCAATATGT TTCCCAGCTG GGAGCACAGA 
GAGGACATCG CTGAGATCAC AGAGATGGAC ACGGAAGTTT CCAATCAGAT CACCCTCGTG 
GAAGACGTCT TAGCCAAGCT CTGTAAAACC ATTTACCCGC TGGCCGACCT CCTGGCCAGG 
CCACTCCCGG AGGGGGTCGA TCCTCTGAAG CTTGAGATCT ATCTCACCGA CGAAGACTTC 
GAGTTTGCAC TAGACATGAC GAGGGATGAA TACAACGCCC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303914.1
CDS	201..5870
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448146.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448146.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGGT GCACTTCACA TTCAGAAACG CCAACTGTCG ATGATGAAGA AAAGGTGGAT 
GAACGAGCCA AGCTGAGCGT CGCCGCCAAG AGGTTGCTTT TCAGGGAGAT GGAAAAATCT 
TTTGATGAAC AAAATGTTCC AAAGCGACGC TCAAGAAACA CAGCTGTGGA GCAGAGGCTA 
CGCCGTCTGC AGGACAGGTC CCTCACCCAG CCCATCACCA CTGAAGAGGT GGTCATCGCA 
GCCACTGAAC CTATCCCCGC TTCGTGTTCT GGGGGCACCC ACCCTGTAAT GGCGAGACTT 
CCTAGCCCCA CTGTAGCTAG GAGCGCTGTG CAGCCTGCCA GATTGCAGGC CTCTGCTCAC 
CAAAAGGCCT TAGCCAAGGA CCAGACAAAT GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA 
CCTGATTCCT CCACTCTAAG CTTGGCCGAA AAGTTGGCCT TGTTTAACAA ATTGTCCCAG 
CCAGTCTCAA AAGCGATTTC TACCCGGAAC AGAATAGACA CGAGACAGAG GAGAATGAAC 
GCTCGCTATC AAACTCAGCC AGTCACACTG GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG 
CTCATTCCTT TCTCACCTGC CGTGAACACA TCAGTGTCTA CCGTAGCATC CACGGTTGCT 
CCAATGTATG CCGGAGATCT TCGCACAAAG CCACCTCTTG ACCACAATGC AAGTGCCACT 
GACTATAAGT TTTCTTCTTC AATAGAAAAT TCGGACTCTC CAGTTAGAAG CATTCTGAAA 
TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT AGCGAGAACA AGGGAATGTT GAGAGAATAT 
GGAGAGACAG AAAGCAAGAG AGCTTTGACA GGTCGAGACA GTGGGATGGA GAAGTATGGG 
TCCTTTGAGG AAGCAGAAGC ATCCTACCCC ATCCTGAACC GAGCCAGGGA AGGAGACAGC 
CATAAGGAAT CTAAATATGC TGTTCCCAGA AGAGGAAGCC TGGAACGGGC GAACCCTCCC 
ATCACCCACC TCGGGGATGA ACCGAAGGAA TTTTCCATGG CTAAAATGAA TGCACAAGGA 
AACTTGGACT TGAGGGACAG GCTGCCCTTT GAAGAGAAGG TGGAGGTGGA GAATGTTATG 
AAAAGGAAGT TTTCACTAAG AGCGGCAGAG TTCGGGGAGC CCACTTCCGA GCAGACGGGG 
ACAGCTGCTG GGAAAACTAT TGCTCAAACC ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT 
TCTTCGGAAC AGCCACAGGA GAAGCTCTGC AAGAATCCAT GTGCGATGTT TGCTGCTGGA 
GAGATCAAAA CGCCGACAGG GGAGGGCCTT CTTGACTCAC CCAGCAAAAC CATGTCTATT 
AAAGAAAGAT TGGCACTGTT GAAGAAAAGC GGGGAGGAAG ATTGGAGAAA CAGACTCAGC 
AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC AGCAGCCTGC ACACCCAGGA AGCAGGGCGG 
TCCCTCATCA AGAAGGAAGA AGGAGGAGTT GCGGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATTC CTGCTGCCCA TAAATACCTG 
TCTTTTGTGT CGATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAAAT GACGATTGAG 
GAGAGGAAGC AGCTCATCAC TGTGAGAGAG GAGGCCTGGA AGACGAGAGG CAGAGGAGCG 
GCCAACGACT CGACCCAGTT CACTGTGGCT GGCAGGATGG TGAAGAAAGG TTTGGCGTCA 
CCTACTGCCA TAACCCCAGT AGCCTCACCC ATTTGCGGTA AAACAAGAGG CACCACACCC 
GTTTCCAAAC CCCTGGAAGA TATCGAAGCC AGACCAGATA TGCAGTTAGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG CGGGATGCAC 
GAAACGGTGC TCACTGTCAC CGGCAAATCT GTGAAGGAGG TGATGAAGCC AGATGATGAT 
GAAACCTTTG CCAAATTTTA CCGCAGCGTG GATTATAATA TGCCAAGAAG TCCTGTGGAG 
ATGGATGAGG ACTTCGATGT CATTTTCGAT CCTTATGCAC CCAAATTGAC GTCTTCCGTG 
GCCGAGCACA AGCGGGCAGT TAGGCCCAAG CGCCGGGTTC AGGCCTCCAA AAACCCCCTG 
AAAATGCTGG CGGCAAGAGA AGATCTCCTT CAGGAATACA CTGAGCAGAG ATTAAACGTT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CTTCCAACTC CAACTTCTCA 
GAAGTCACCC TGGCGGGTTT AGCCAGTAAA GAAAACTTCA GCAACGTCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA CTCTAACAAC AGCGCCGTGC CCTACAAGAG GCTGATGCTG 
TTGCAGATTA AAGGAAGAAG ACATGTGCAG ACCAGGCTGG TGGAACCTCG AGCTTCGGCG 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCTCCCCACT GCTGCTTCCT GTGGGTAGGA 
GAGTTTGCAA ACGTCATAGA AAAGGCGAAG GCCTCAGAAC TTGCAACTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACTTAT ATCCAAACCA TTGAAGAAGG AATTAATACA 
CACACTCATG CAGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAATCT 
GCTGGAGACC CAAAAGAAGA TGAACTCTAT GAAGCAGCCA TAATAGAAAC TAACTGCATT 
TACCGTCTCA TGGATGACAA ACTTGTTCCT GATGACGACT ACTGGGGGAA AATTCCGAAG 
TGCTCCCTTC TGCAACCCAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTTTACGTA 
TGGCATGGGA AAGAAGTCAC ATTAGCACAA CGAAAAATAG CATTTCAGCT GGCAAAGCAC 
TTATGGAATG GAACCTTTGA CTATGAGAAC TGTGACATCA ATCCCCTGGA TCCTGGAGAA 
TGCAATCCGC TTATCCCCAG AAAAGGACAG GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA 
CTTACTGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAGT TTCTGGATTG GACGGAACTG 
AAGAGATCGA ATGAGAAGAA CCCCGGGGAA CTTGCCCAGC ACAAGGAAGA CCCCAGGACT 
GATGTCAAGG CATACGATGT GACACGGATG GTGTCCATGC CCCAGACGAC AGCAGGCACC 
ATCCTGGACG GAGTGAACGT CGGCCGTGGC TATGGCCTGG TGGAAGGACA CGACAGGAGG 
CAGTTTGAGA TCACCAGCGT TTCCGTGGAT GTCTGGCACA TCCTGGAATT CGACTATAGC 
AGGCTCCCCA AACAAAGCAT CGGGCAGTTC CATGAGGGGG ATGCCTATGT GGTCAAGTGG 
AAGTTCATGG TGAGCACGGC AGTGGGAAGT CGCCAGAAGG GAGAGCACTC GGTGAGGGCA 
GCCGGCAAAG AGAAGTGCGT CTACTTCTTC TGGCAAGGCC GGCACTCCAC CGTGAGTGAG 
AAGGGCACGT CGGCGCTGAT GACGGTGGAG CTGGACGAGG AAAGGGGGGC CCAGGTCCAG 
GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC CTGCAGTGTT TCCAGGGGGG GATGGTGGTG 
CACTCGGGGA GGCGGGAAGA GGAAGAAGAA AATGTGCAAA GTGAGTGGCG GCTGTACTGC 
GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC 
CTGAGGTCCA GAACTTCCAT GGTGGTGCTT AACGTCAACA AGGCCCTCAT CTACCTGTGG 
CACGGATGCA AAGCCCAGGC CCACACGAAG GAGGTCGGAA GGACCGCTGC GAACAAGATC 
AAGGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTCAC AATACACGAG 
TGTGATGAAG GCTCCGAGCC ACTCGGATTC TGGGATGCCT TAGGAAGGAG AGACAGGAAA 
GCCTACGATT GCATGCTTCA AGATCCTGGA AGTTTTAACT TCGCGCCCCG CCTGTTCATC 
CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC ACAGAGTTTG TGTACCCTGC CCGAGCCCCC 
TCTGTGGTCA GTTCCATGCC CTTCCTGCAG GAAGATCTGT ACAGCGCGCC CCAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTG TACCTCTGGC AAGGCTGGTG GCCCATCGAG 
AACAAGATCA CTGGTTCCGC CCGCATCCGC TGGGCCTCCG ACCGGAAGAG TGCGATGGAG 
ACTGTGCTCC AGTACTGCAA AGGAAAAAAT CTCAAGAAAC CAGCCCCCAA GTCTTACCTT 
ATCCACGCTG GTCTGGAGCC CCTGACATTC ACCAATATGT TTCCCAGCTG GGAGCACAGA 
GAGGACATCG CTGAGATCAC AGAGATGGAC ACGGAAGTTT CCAATCAGAT CACCCTCGTG 
GAAGACGTCT TAGCCAAGCT CTGTAAAACC ATTTACCCGC TGGCCGACCT CCTGGCCAGG 
CCACTCCCGG AGGGGGTCGA TCCTCTGAAG CTTGAGATCT ATCTCACCGA CGAAGACTTC 
GAGTTTGCAC TAGACATGAC GAGGGATGAA TACAACGCCC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448144.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303912.1 Homo sapiens supervillin (SVIL), transcript variant X12, mRNA.

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CloneID	OHu60826
Clone ID Related Accession (Same CDS sequence)	XM_024448144.1
Accession Version	XM_024448144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGCA AATCACGGGT GGAGAGGTCG GCTGAAGGAC CTGGCTTGCC CACCGGTGTG 
GAACGGGAGA GAGGGTCCCG GAAACCAAGA CGCTATTTTT CTCCTGGTGA AAGTAGAAAA 
ACTTCCGAGA GATTTAGAAC CCAACCTATA ACTTCAGCAG AACGAAAGGA ATCGGATAGG 
TGCACTTCAC ATTCAGAAAC GCCAACTGTC GATGATGAAG AAAAGGTGGA TGAACGAGCC 
AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT TTCAGGGAGA TGGAAAAATC TTTTGATGAA 
CAAAATGTTC CAAAGCGACG CTCAAGAAAC ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG 
CAGGACAGGT CCCTCACCCA GCCCATCACC ACTGAAGAGG TGGTCATCGC AGCCACTGAA 
CCTATCCCCG CTTCGTGTTC TGGGGGCACC CACCCTGTAA TGGCGAGACT TCCTAGCCCC 
ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC AGATTGCAGG CCTCTGCTCA CCAAAAGGCC 
TTAGCCAAGG ACCAGACAAA TGAGGGCAAA GAGCTTGCTG AGCAAGGAGA ACCTGATTCC 
TCCACTCTAA GCTTGGCCGA AAAGTTGGCC TTGTTTAACA AATTGTCCCA GCCAGTCTCA 
AAAGCGATTT CTACCCGGAA CAGAATAGAC ACGAGACAGA GGAGAATGAA CGCTCGCTAT 
CAAACTCAGC CAGTCACACT GGGAGAGGTG GAGCAGGTGC AGAGTGGAAA GCTCATTCCT 
TTCTCACCTG CCGTGAACAC ATCAGTGTCT ACCGTAGCAT CCACGGTTGC TCCAATGTAT 
GCCGGAGATC TTCGCACAAA GCCACCTCTT GACCACAATG CAAGTGCCAC TGACTATAAG 
TTTTCTTCTT CAATAGAAAA TTCGGACTCT CCAGTTAGAA GCATTCTGAA ATCGCAAGCT 
TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC AAGGGAATGT TGAGAGAATA TGGAGAGACA 
GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC AGTGGGATGG AGAAGTATGG GTCCTTTGAG 
GAAGCAGAAG CATCCTACCC CATCCTGAAC CGAGCCAGGG AAGGAGACAG CCATAAGGAA 
TCTAAATATG CTGTTCCCAG AAGAGGAAGC CTGGAACGGG CGAACCCTCC CATCACCCAC 
CTCGGGGATG AACCGAAGGA ATTTTCCATG GCTAAAATGA ATGCACAAGG AAACTTGGAC 
TTGAGGGACA GGCTGCCCTT TGAAGAGAAG GTGGAGGTGG AGAATGTTAT GAAAAGGAAG 
TTTTCACTAA GAGCGGCAGA GTTCGGGGAG CCCACTTCCG AGCAGACGGG GACAGCTGCT 
GGGAAAACTA TTGCTCAAAC CACAGCCCCC GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA 
CAGCCACAGG AGAAGCTCTG CAAGAATCCA TGTGCGATGT TTGCTGCTGG AGAGATCAAA 
ACGCCGACAG GGGAGGGCCT TCTTGACTCA CCCAGCAAAA CCATGTCTAT TAAAGAAAGA 
TTGGCACTGT TGAAGAAAAG CGGGGAGGAA GATTGGAGAA ACAGACTCAG CAGGAGGCAG 
GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG CACACCCAGG AAGCAGGGCG GTCCCTCATC 
AAGAAGGAAG AAGGAGGAGT TGCGGATGAT AGTGCCATTT CTAATCTGCT TTGGGAACCT 
GTATATGCTT CTACTTATTC TCCTGCTATT CCTGCTGCCC ATAAATACCT GTCTTTTGTG 
TCGATTAATC AGCGGGTCAC AGAAAGTCGA GAGAGCCAAA TGACGATTGA GGAGAGGAAG 
CAGCTCATCA CTGTGAGAGA GGAGGCCTGG AAGACGAGAG GCAGAGGAGC GGCCAACGAC 
TCGACCCAGT TCACTGTGGC TGGCAGGATG GTGAAGAAAG GTTTGGCGTC ACCTACTGCC 
ATAACCCCAG TAGCCTCACC CATTTGCGGT AAAACAAGAG GCACCACACC CGTTTCCAAA 
CCCCTGGAAG ATATCGAAGC CAGACCAGAT ATGCAGTTAG AATCGGACCT GAAGTTGGAC 
AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT AACAAAGTTG GCGGGATGCA CGAAACGGTG 
CTCACTGTCA CCGGCAAATC TGTGAAGGAG GTGATGAAGC CAGATGATGA TGAAACCTTT 
GCCAAATTTT ACCGCAGCGT GGATTATAAT ATGCCAAGAA GTCCTGTGGA GATGGATGAG 
GACTTCGATG TCATTTTCGA TCCTTATGCA CCCAAATTGA CGTCTTCCGT GGCCGAGCAC 
AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT CAGGCCTCCA AAAACCCCCT GAAAATGCTG 
GCGGCAAGAG AAGATCTCCT TCAGGAATAC ACTGAGCAGA GATTAAACGT TGCCTTCATG 
GAGTCAAAGC GGATGAAAGT AGAAAAGATG TCTTCCAACT CCAACTTCTC AGAAGTCACC 
CTGGCGGGTT TAGCCAGTAA AGAAAACTTC AGCAACGTCA GCCTGCGGAG CGTCAACCTG 
ACGGAACAGA ACTCTAACAA CAGCGCCGTG CCCTACAAGA GGCTGATGCT GTTGCAGATT 
AAAGGAAGAA GACATGTGCA GACCAGGCTG GTGGAACCTC GAGCTTCGGC GCTCAACAGT 
GGGGACTGCT TCCTCCTGCT CTCTCCCCAC TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA 
AACGTCATAG AAAAGGCGAA GGCCTCAGAA CTTGCAACTT TAATTCAGAC AAAGAGGGAA 
CTTGGTTGTA GAGCTACTTA TATCCAAACC ATTGAAGAAG GAATTAATAC ACACACTCAT 
GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT GGCCAAACCA GTTACCAATC TGCTGGAGAC 
CCAAAAGAAG ATGAACTCTA TGAAGCAGCC ATAATAGAAA CTAACTGCAT TTACCGTCTC 
ATGGATGACA AACTTGTTCC TGATGACGAC TACTGGGGGA AAATTCCGAA GTGCTCCCTT 
CTGCAACCCA AAGAGGTACT GGTGTTTGAT TTTGGTAGTG AAGTTTACGT ATGGCATGGG 
AAAGAAGTCA CATTAGCACA ACGAAAAATA GCATTTCAGC TGGCAAAGCA CTTATGGAAT 
GGAACCTTTG ACTATGAGAA CTGTGACATC AATCCCCTGG ATCCTGGAGA ATGCAATCCG 
CTTATCCCCA GAAAAGGACA GGGGCGGCCC GACTGGGCGA TATTTGGGAG ACTTACTGAA 
CACAATGAGA CGATTTTGTT CAAAGAGAAG TTTCTGGATT GGACGGAACT GAAGAGATCG 
AATGAGAAGA ACCCCGGGGA ACTTGCCCAG CACAAGGAAG ACCCCAGGAC TGATGTCAAG 
GCATACGATG TGACACGGAT GGTGTCCATG CCCCAGACGA CAGCAGGCAC CATCCTGGAC 
GGAGTGAACG TCGGCCGTGG CTATGGCCTG GTGGAAGGAC ACGACAGGAG GCAGTTTGAG 
ATCACCAGCG TTTCCGTGGA TGTCTGGCAC ATCCTGGAAT TCGACTATAG CAGGCTCCCC 
AAACAAAGCA TCGGGCAGTT CCATGAGGGG GATGCCTATG TGGTCAAGTG GAAGTTCATG 
GTGAGCACGG CAGTGGGAAG TCGCCAGAAG GGAGAGCACT CGGTGAGGGC AGCCGGCAAA 
GAGAAGTGCG TCTACTTCTT CTGGCAAGGC CGGCACTCCA CCGTGAGTGA GAAGGGCACG 
TCGGCGCTGA TGACGGTGGA GCTGGACGAG GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG 
GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT TTCCAGGGGG GGATGGTGGT GCACTCGGGG 
AGGCGGGAAG AGGAAGAAGA AAATGTGCAA AGTGAGTGGC GGCTGTACTG CGTGCGTGGA 
GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC 
AGAACTTCCA TGGTGGTGCT TAACGTCAAC AAGGCCCTCA TCTACCTGTG GCACGGATGC 
AAAGCCCAGG CCCACACGAA GGAGGTCGGA AGGACCGCTG CGAACAAGAT CAAGGAACAA 
TGTCCCCTGG AAGCAGGACT GCATAGTAGC AGCAAAGTCA CAATACACGA GTGTGATGAA 
GGCTCCGAGC CACTCGGATT CTGGGATGCC TTAGGAAGGA GAGACAGGAA AGCCTACGAT 
TGCATGCTTC AAGATCCTGG AAGTTTTAAC TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC 
TCCTCTGGGG ATTTTGCAGC CACAGAGTTT GTGTACCCTG CCCGAGCCCC CTCTGTGGTC 
AGTTCCATGC CCTTCCTGCA GGAAGATCTG TACAGCGCGC CCCAGCCAGC ACTTTTCCTT 
GTTGACAATC ACCACGAGGT GTACCTCTGG CAAGGCTGGT GGCCCATCGA GAACAAGATC 
ACTGGTTCCG CCCGCATCCG CTGGGCCTCC GACCGGAAGA GTGCGATGGA GACTGTGCTC 
CAGTACTGCA AAGGAAAAAA TCTCAAGAAA CCAGCCCCCA AGTCTTACCT TATCCACGCT 
GGTCTGGAGC CCCTGACATT CACCAATATG TTTCCCAGCT GGGAGCACAG AGAGGACATC 
GCTGAGATCA CAGAGATGGA CACGGAAGTT TCCAATCAGA TCACCCTCGT GGAAGACGTC 
TTAGCCAAGC TCTGTAAAAC CATTTACCCG CTGGCCGACC TCCTGGCCAG GCCACTCCCG 
GAGGGGGTCG ATCCTCTGAA GCTTGAGATC TATCTCACCG ACGAAGACTT CGAGTTTGCA 
CTAGACATGA CGAGGGATGA ATACAACGCC CTGCCCGCCT GGAAGCAGGT GAACCTGAAG 
AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303912.1
CDS	201..6041
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSSKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448144.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448144.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGCA AATCACGGGT GGAGAGGTCG GCTGAAGGAC CTGGCTTGCC CACCGGTGTG 
GAACGGGAGA GAGGGTCCCG GAAACCAAGA CGCTATTTTT CTCCTGGTGA AAGTAGAAAA 
ACTTCCGAGA GATTTAGAAC CCAACCTATA ACTTCAGCAG AACGAAAGGA ATCGGATAGG 
TGCACTTCAC ATTCAGAAAC GCCAACTGTC GATGATGAAG AAAAGGTGGA TGAACGAGCC 
AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT TTCAGGGAGA TGGAAAAATC TTTTGATGAA 
CAAAATGTTC CAAAGCGACG CTCAAGAAAC ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG 
CAGGACAGGT CCCTCACCCA GCCCATCACC ACTGAAGAGG TGGTCATCGC AGCCACTGAA 
CCTATCCCCG CTTCGTGTTC TGGGGGCACC CACCCTGTAA TGGCGAGACT TCCTAGCCCC 
ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC AGATTGCAGG CCTCTGCTCA CCAAAAGGCC 
TTAGCCAAGG ACCAGACAAA TGAGGGCAAA GAGCTTGCTG AGCAAGGAGA ACCTGATTCC 
TCCACTCTAA GCTTGGCCGA AAAGTTGGCC TTGTTTAACA AATTGTCCCA GCCAGTCTCA 
AAAGCGATTT CTACCCGGAA CAGAATAGAC ACGAGACAGA GGAGAATGAA CGCTCGCTAT 
CAAACTCAGC CAGTCACACT GGGAGAGGTG GAGCAGGTGC AGAGTGGAAA GCTCATTCCT 
TTCTCACCTG CCGTGAACAC ATCAGTGTCT ACCGTAGCAT CCACGGTTGC TCCAATGTAT 
GCCGGAGATC TTCGCACAAA GCCACCTCTT GACCACAATG CAAGTGCCAC TGACTATAAG 
TTTTCTTCTT CAATAGAAAA TTCGGACTCT CCAGTTAGAA GCATTCTGAA ATCGCAAGCT 
TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC AAGGGAATGT TGAGAGAATA TGGAGAGACA 
GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC AGTGGGATGG AGAAGTATGG GTCCTTTGAG 
GAAGCAGAAG CATCCTACCC CATCCTGAAC CGAGCCAGGG AAGGAGACAG CCATAAGGAA 
TCTAAATATG CTGTTCCCAG AAGAGGAAGC CTGGAACGGG CGAACCCTCC CATCACCCAC 
CTCGGGGATG AACCGAAGGA ATTTTCCATG GCTAAAATGA ATGCACAAGG AAACTTGGAC 
TTGAGGGACA GGCTGCCCTT TGAAGAGAAG GTGGAGGTGG AGAATGTTAT GAAAAGGAAG 
TTTTCACTAA GAGCGGCAGA GTTCGGGGAG CCCACTTCCG AGCAGACGGG GACAGCTGCT 
GGGAAAACTA TTGCTCAAAC CACAGCCCCC GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA 
CAGCCACAGG AGAAGCTCTG CAAGAATCCA TGTGCGATGT TTGCTGCTGG AGAGATCAAA 
ACGCCGACAG GGGAGGGCCT TCTTGACTCA CCCAGCAAAA CCATGTCTAT TAAAGAAAGA 
TTGGCACTGT TGAAGAAAAG CGGGGAGGAA GATTGGAGAA ACAGACTCAG CAGGAGGCAG 
GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG CACACCCAGG AAGCAGGGCG GTCCCTCATC 
AAGAAGGAAG AAGGAGGAGT TGCGGATGAT AGTGCCATTT CTAATCTGCT TTGGGAACCT 
GTATATGCTT CTACTTATTC TCCTGCTATT CCTGCTGCCC ATAAATACCT GTCTTTTGTG 
TCGATTAATC AGCGGGTCAC AGAAAGTCGA GAGAGCCAAA TGACGATTGA GGAGAGGAAG 
CAGCTCATCA CTGTGAGAGA GGAGGCCTGG AAGACGAGAG GCAGAGGAGC GGCCAACGAC 
TCGACCCAGT TCACTGTGGC TGGCAGGATG GTGAAGAAAG GTTTGGCGTC ACCTACTGCC 
ATAACCCCAG TAGCCTCACC CATTTGCGGT AAAACAAGAG GCACCACACC CGTTTCCAAA 
CCCCTGGAAG ATATCGAAGC CAGACCAGAT ATGCAGTTAG AATCGGACCT GAAGTTGGAC 
AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT AACAAAGTTG GCGGGATGCA CGAAACGGTG 
CTCACTGTCA CCGGCAAATC TGTGAAGGAG GTGATGAAGC CAGATGATGA TGAAACCTTT 
GCCAAATTTT ACCGCAGCGT GGATTATAAT ATGCCAAGAA GTCCTGTGGA GATGGATGAG 
GACTTCGATG TCATTTTCGA TCCTTATGCA CCCAAATTGA CGTCTTCCGT GGCCGAGCAC 
AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT CAGGCCTCCA AAAACCCCCT GAAAATGCTG 
GCGGCAAGAG AAGATCTCCT TCAGGAATAC ACTGAGCAGA GATTAAACGT TGCCTTCATG 
GAGTCAAAGC GGATGAAAGT AGAAAAGATG TCTTCCAACT CCAACTTCTC AGAAGTCACC 
CTGGCGGGTT TAGCCAGTAA AGAAAACTTC AGCAACGTCA GCCTGCGGAG CGTCAACCTG 
ACGGAACAGA ACTCTAACAA CAGCGCCGTG CCCTACAAGA GGCTGATGCT GTTGCAGATT 
AAAGGAAGAA GACATGTGCA GACCAGGCTG GTGGAACCTC GAGCTTCGGC GCTCAACAGT 
GGGGACTGCT TCCTCCTGCT CTCTCCCCAC TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA 
AACGTCATAG AAAAGGCGAA GGCCTCAGAA CTTGCAACTT TAATTCAGAC AAAGAGGGAA 
CTTGGTTGTA GAGCTACTTA TATCCAAACC ATTGAAGAAG GAATTAATAC ACACACTCAT 
GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT GGCCAAACCA GTTACCAATC TGCTGGAGAC 
CCAAAAGAAG ATGAACTCTA TGAAGCAGCC ATAATAGAAA CTAACTGCAT TTACCGTCTC 
ATGGATGACA AACTTGTTCC TGATGACGAC TACTGGGGGA AAATTCCGAA GTGCTCCCTT 
CTGCAACCCA AAGAGGTACT GGTGTTTGAT TTTGGTAGTG AAGTTTACGT ATGGCATGGG 
AAAGAAGTCA CATTAGCACA ACGAAAAATA GCATTTCAGC TGGCAAAGCA CTTATGGAAT 
GGAACCTTTG ACTATGAGAA CTGTGACATC AATCCCCTGG ATCCTGGAGA ATGCAATCCG 
CTTATCCCCA GAAAAGGACA GGGGCGGCCC GACTGGGCGA TATTTGGGAG ACTTACTGAA 
CACAATGAGA CGATTTTGTT CAAAGAGAAG TTTCTGGATT GGACGGAACT GAAGAGATCG 
AATGAGAAGA ACCCCGGGGA ACTTGCCCAG CACAAGGAAG ACCCCAGGAC TGATGTCAAG 
GCATACGATG TGACACGGAT GGTGTCCATG CCCCAGACGA CAGCAGGCAC CATCCTGGAC 
GGAGTGAACG TCGGCCGTGG CTATGGCCTG GTGGAAGGAC ACGACAGGAG GCAGTTTGAG 
ATCACCAGCG TTTCCGTGGA TGTCTGGCAC ATCCTGGAAT TCGACTATAG CAGGCTCCCC 
AAACAAAGCA TCGGGCAGTT CCATGAGGGG GATGCCTATG TGGTCAAGTG GAAGTTCATG 
GTGAGCACGG CAGTGGGAAG TCGCCAGAAG GGAGAGCACT CGGTGAGGGC AGCCGGCAAA 
GAGAAGTGCG TCTACTTCTT CTGGCAAGGC CGGCACTCCA CCGTGAGTGA GAAGGGCACG 
TCGGCGCTGA TGACGGTGGA GCTGGACGAG GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG 
GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT TTCCAGGGGG GGATGGTGGT GCACTCGGGG 
AGGCGGGAAG AGGAAGAAGA AAATGTGCAA AGTGAGTGGC GGCTGTACTG CGTGCGTGGA 
GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC 
AGAACTTCCA TGGTGGTGCT TAACGTCAAC AAGGCCCTCA TCTACCTGTG GCACGGATGC 
AAAGCCCAGG CCCACACGAA GGAGGTCGGA AGGACCGCTG CGAACAAGAT CAAGGAACAA 
TGTCCCCTGG AAGCAGGACT GCATAGTAGC AGCAAAGTCA CAATACACGA GTGTGATGAA 
GGCTCCGAGC CACTCGGATT CTGGGATGCC TTAGGAAGGA GAGACAGGAA AGCCTACGAT 
TGCATGCTTC AAGATCCTGG AAGTTTTAAC TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC 
TCCTCTGGGG ATTTTGCAGC CACAGAGTTT GTGTACCCTG CCCGAGCCCC CTCTGTGGTC 
AGTTCCATGC CCTTCCTGCA GGAAGATCTG TACAGCGCGC CCCAGCCAGC ACTTTTCCTT 
GTTGACAATC ACCACGAGGT GTACCTCTGG CAAGGCTGGT GGCCCATCGA GAACAAGATC 
ACTGGTTCCG CCCGCATCCG CTGGGCCTCC GACCGGAAGA GTGCGATGGA GACTGTGCTC 
CAGTACTGCA AAGGAAAAAA TCTCAAGAAA CCAGCCCCCA AGTCTTACCT TATCCACGCT 
GGTCTGGAGC CCCTGACATT CACCAATATG TTTCCCAGCT GGGAGCACAG AGAGGACATC 
GCTGAGATCA CAGAGATGGA CACGGAAGTT TCCAATCAGA TCACCCTCGT GGAAGACGTC 
TTAGCCAAGC TCTGTAAAAC CATTTACCCG CTGGCCGACC TCCTGGCCAG GCCACTCCCG 
GAGGGGGTCG ATCCTCTGAA GCTTGAGATC TATCTCACCG ACGAAGACTT CGAGTTTGCA 
CTAGACATGA CGAGGGATGA ATACAACGCC CTGCCCGCCT GGAAGCAGGT GAACCTGAAG 
AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448147.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303915.1 Homo sapiens supervillin (SVIL), transcript variant X15, mRNA.

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CloneID	OHu60827
Clone ID Related Accession (Same CDS sequence)	XM_024448147.1
Accession Version	XM_024448147.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGGTG CACTTCACAT 
TCAGAAACGC CAACTGTCGA TGATGAAGAA AAGGTGGATG AACGAGCCAA GCTGAGCGTC 
GCCGCCAAGA GGTTGCTTTT CAGGGAGATG GAAAAATCTT TTGATGAACA AAATGTTCCA 
AAGCGACGCT CAAGAAACAC AGCTGTGGAG CAGAGGCTAC GCCGTCTGCA GGACAGGTCC 
CTCACCCAGC CCATCACCAC TGAAGAGGTG GTCATCGCAG CCACTGAACC TATCCCCGCT 
TCGTGTTCTG GGGGCACCCA CCCTGTAATG GCGAGACTTC CTAGCCCCAC TGTAGCTAGG 
AGCGCTGTGC AGCCTGCCAG ATTGCAGGCC TCTGCTCACC AAAAGGCCTT AGCCAAGGAC 
CAGACAAATG AGGGCAAAGA GCTTGCTGAG CAAGGAGAAC CTGATTCCTC CACTCTAAGC 
TTGGCCGAAA AGTTGGCCTT GTTTAACAAA TTGTCCCAGC CAGTCTCAAA AGCGATTTCT 
ACCCGGAACA GAATAGACAC GAGACAGAGG AGAATGAACG CTCGCTATCA AACTCAGCCA 
GTCACACTGG GAGAGGTGGA GCAGGTGCAG AGTGGAAAGC TCATTCCTTT CTCACCTGCC 
GTGAACACAT CAGTGTCTAC CGTAGCATCC ACGGTTGCTC CAATGTATGC CGGAGATCTT 
CGCACAAAGC CACCTCTTGA CCACAATGCA AGTGCCACTG ACTATAAGTT TTCTTCTTCA 
ATAGAAAATT CGGACTCTCC AGTTAGAAGC ATTCTGAAAT CGCAAGCTTG GCAGCCTTTG 
GTAGAGGGTA GCGAGAACAA GGGAATGTTG AGAGAATATG GAGAGACAGA AAGCAAGAGA 
GCTTTGACAG GTCGAGACAG TGGGATGGAG AAGTATGGGT CCTTTGAGGA AGCAGAAGCA 
TCCTACCCCA TCCTGAACCG AGCCAGGGAA GGAGACAGCC ATAAGGAATC TAAATATGCT 
GTTCCCAGAA GAGGAAGCCT GGAACGGGCG AACCCTCCCA TCACCCACCT CGGGGATGAA 
CCGAAGGAAT TTTCCATGGC TAAAATGAAT GCACAAGGAA ACTTGGACTT GAGGGACAGG 
CTGCCCTTTG AAGAGAAGGT GGAGGTGGAG AATGTTATGA AAAGGAAGTT TTCACTAAGA 
GCGGCAGAGT TCGGGGAGCC CACTTCCGAG CAGACGGGGA CAGCTGCTGG GAAAACTATT 
GCTCAAACCA CAGCCCCCGT GTCCTGGAAG CCCCAGGATT CTTCGGAACA GCCACAGGAG 
AAGCTCTGCA AGAATCCATG TGCGATGTTT GCTGCTGGAG AGATCAAAAC GCCGACAGGG 
GAGGGCCTTC TTGACTCACC CAGCAAAACC ATGTCTATTA AAGAAAGATT GGCACTGTTG 
AAGAAAAGCG GGGAGGAAGA TTGGAGAAAC AGACTCAGCA GGAGGCAGGA GGGCGGCAAG 
GCGCCGGCCA GCAGCCTGCA CACCCAGGAA GCAGGGCGGT CCCTCATCAA GAAGGAAGAA 
GGAGGAGTTG CGGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATTCC TGCTGCCCAT AAATACCTGT CTTTTGTGTC GATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAAATG ACGATTGAGG AGAGGAAGCA GCTCATCACT 
GTGAGAGAGG AGGCCTGGAA GACGAGAGGC AGAGGAGCGG CCAACGACTC GACCCAGTTC 
ACTGTGGCTG GCAGGATGGT GAAGAAAGGT TTGGCGTCAC CTACTGCCAT AACCCCAGTA 
GCCTCACCCA TTTGCGGTAA AACAAGAGGC ACCACACCCG TTTCCAAACC CCTGGAAGAT 
ATCGAAGCCA GACCAGATAT GCAGTTAGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGC GGGATGCACG AAACGGTGCT CACTGTCACC 
GGCAAATCTG TGAAGGAGGT GATGAAGCCA GATGATGATG AAACCTTTGC CAAATTTTAC 
CGCAGCGTGG ATTATAATAT GCCAAGAAGT CCTGTGGAGA TGGATGAGGA CTTCGATGTC 
ATTTTCGATC CTTATGCACC CAAATTGACG TCTTCCGTGG CCGAGCACAA GCGGGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCCTCCAAA AACCCCCTGA AAATGCTGGC GGCAAGAGAA 
GATCTCCTTC AGGAATACAC TGAGCAGAGA TTAAACGTTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC TTCCAACTCC AACTTCTCAG AAGTCACCCT GGCGGGTTTA 
GCCAGTAAAG AAAACTTCAG CAACGTCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAC 
TCTAACAACA GCGCCGTGCC CTACAAGAGG CTGATGCTGT TGCAGATTAA AGGAAGAAGA 
CATGTGCAGA CCAGGCTGGT GGAACCTCGA GCTTCGGCGC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CTCCCCACTG CTGCTTCCTG TGGGTAGGAG AGTTTGCAAA CGTCATAGAA 
AAGGCGAAGG CCTCAGAACT TGCAACTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACTTATA TCCAAACCAT TGAAGAAGGA ATTAATACAC ACACTCATGC AGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAATCTG CTGGAGACCC AAAAGAAGAT 
GAACTCTATG AAGCAGCCAT AATAGAAACT AACTGCATTT ACCGTCTCAT GGATGACAAA 
CTTGTTCCTG ATGACGACTA CTGGGGGAAA ATTCCGAAGT GCTCCCTTCT GCAACCCAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTTTACGTAT GGCATGGGAA AGAAGTCACA 
TTAGCACAAC GAAAAATAGC ATTTCAGCTG GCAAAGCACT TATGGAATGG AACCTTTGAC 
TATGAGAACT GTGACATCAA TCCCCTGGAT CCTGGAGAAT GCAATCCGCT TATCCCCAGA 
AAAGGACAGG GGCGGCCCGA CTGGGCGATA TTTGGGAGAC TTACTGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAGTT TCTGGATTGG ACGGAACTGA AGAGATCGAA TGAGAAGAAC 
CCCGGGGAAC TTGCCCAGCA CAAGGAAGAC CCCAGGACTG ATGTCAAGGC ATACGATGTG 
ACACGGATGG TGTCCATGCC CCAGACGACA GCAGGCACCA TCCTGGACGG AGTGAACGTC 
GGCCGTGGCT ATGGCCTGGT GGAAGGACAC GACAGGAGGC AGTTTGAGAT CACCAGCGTT 
TCCGTGGATG TCTGGCACAT CCTGGAATTC GACTATAGCA GGCTCCCCAA ACAAAGCATC 
GGGCAGTTCC ATGAGGGGGA TGCCTATGTG GTCAAGTGGA AGTTCATGGT GAGCACGGCA 
GTGGGAAGTC GCCAGAAGGG AGAGCACTCG GTGAGGGCAG CCGGCAAAGA GAAGTGCGTC 
TACTTCTTCT GGCAAGGCCG GCACTCCACC GTGAGTGAGA AGGGCACGTC GGCGCTGATG 
ACGGTGGAGC TGGACGAGGA AAGGGGGGCC CAGGTCCAGG TTCTCCAGGG AAAGGAGCCC 
CCCTGTTTCC TGCAGTGTTT CCAGGGGGGG ATGGTGGTGC ACTCGGGGAG GCGGGAAGAG 
GAAGAAGAAA ATGTGCAAAG TGAGTGGCGG CTGTACTGCG TGCGTGGAGA GGTGCCCGTG 
GAAGGGAATT TGCTGGAAGT GGCCTGTCAC TGTAGCAGCC TGAGGTCCAG AACTTCCATG 
GTGGTGCTTA ACGTCAACAA GGCCCTCATC TACCTGTGGC ACGGATGCAA AGCCCAGGCC 
CACACGAAGG AGGTCGGAAG GACCGCTGCG AACAAGATCA AGGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTCACA ATACACGAGT GTGATGAAGG CTCCGAGCCA 
CTCGGATTCT GGGATGCCTT AGGAAGGAGA GACAGGAAAG CCTACGATTG CATGCTTCAA 
GATCCTGGAA GTTTTAACTT CGCGCCCCGC CTGTTCATCC TCAGCAGCTC CTCTGGGGAT 
TTTGCAGCCA CAGAGTTTGT GTACCCTGCC CGAGCCCCCT CTGTGGTCAG TTCCATGCCC 
TTCCTGCAGG AAGATCTGTA CAGCGCGCCC CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTGT ACCTCTGGCA AGGCTGGTGG CCCATCGAGA ACAAGATCAC TGGTTCCGCC 
CGCATCCGCT GGGCCTCCGA CCGGAAGAGT GCGATGGAGA CTGTGCTCCA GTACTGCAAA 
GGAAAAAATC TCAAGAAACC AGCCCCCAAG TCTTACCTTA TCCACGCTGG TCTGGAGCCC 
CTGACATTCA CCAATATGTT TCCCAGCTGG GAGCACAGAG AGGACATCGC TGAGATCACA 
GAGATGGACA CGGAAGTTTC CAATCAGATC ACCCTCGTGG AAGACGTCTT AGCCAAGCTC 
TGTAAAACCA TTTACCCGCT GGCCGACCTC CTGGCCAGGC CACTCCCGGA GGGGGTCGAT 
CCTCTGAAGC TTGAGATCTA TCTCACCGAC GAAGACTTCG AGTTTGCACT AGACATGACG 
AGGGATGAAT ACAACGCCCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAAGGC 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303915.1
CDS	201..5789
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448147.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448147.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGGTG CACTTCACAT 
TCAGAAACGC CAACTGTCGA TGATGAAGAA AAGGTGGATG AACGAGCCAA GCTGAGCGTC 
GCCGCCAAGA GGTTGCTTTT CAGGGAGATG GAAAAATCTT TTGATGAACA AAATGTTCCA 
AAGCGACGCT CAAGAAACAC AGCTGTGGAG CAGAGGCTAC GCCGTCTGCA GGACAGGTCC 
CTCACCCAGC CCATCACCAC TGAAGAGGTG GTCATCGCAG CCACTGAACC TATCCCCGCT 
TCGTGTTCTG GGGGCACCCA CCCTGTAATG GCGAGACTTC CTAGCCCCAC TGTAGCTAGG 
AGCGCTGTGC AGCCTGCCAG ATTGCAGGCC TCTGCTCACC AAAAGGCCTT AGCCAAGGAC 
CAGACAAATG AGGGCAAAGA GCTTGCTGAG CAAGGAGAAC CTGATTCCTC CACTCTAAGC 
TTGGCCGAAA AGTTGGCCTT GTTTAACAAA TTGTCCCAGC CAGTCTCAAA AGCGATTTCT 
ACCCGGAACA GAATAGACAC GAGACAGAGG AGAATGAACG CTCGCTATCA AACTCAGCCA 
GTCACACTGG GAGAGGTGGA GCAGGTGCAG AGTGGAAAGC TCATTCCTTT CTCACCTGCC 
GTGAACACAT CAGTGTCTAC CGTAGCATCC ACGGTTGCTC CAATGTATGC CGGAGATCTT 
CGCACAAAGC CACCTCTTGA CCACAATGCA AGTGCCACTG ACTATAAGTT TTCTTCTTCA 
ATAGAAAATT CGGACTCTCC AGTTAGAAGC ATTCTGAAAT CGCAAGCTTG GCAGCCTTTG 
GTAGAGGGTA GCGAGAACAA GGGAATGTTG AGAGAATATG GAGAGACAGA AAGCAAGAGA 
GCTTTGACAG GTCGAGACAG TGGGATGGAG AAGTATGGGT CCTTTGAGGA AGCAGAAGCA 
TCCTACCCCA TCCTGAACCG AGCCAGGGAA GGAGACAGCC ATAAGGAATC TAAATATGCT 
GTTCCCAGAA GAGGAAGCCT GGAACGGGCG AACCCTCCCA TCACCCACCT CGGGGATGAA 
CCGAAGGAAT TTTCCATGGC TAAAATGAAT GCACAAGGAA ACTTGGACTT GAGGGACAGG 
CTGCCCTTTG AAGAGAAGGT GGAGGTGGAG AATGTTATGA AAAGGAAGTT TTCACTAAGA 
GCGGCAGAGT TCGGGGAGCC CACTTCCGAG CAGACGGGGA CAGCTGCTGG GAAAACTATT 
GCTCAAACCA CAGCCCCCGT GTCCTGGAAG CCCCAGGATT CTTCGGAACA GCCACAGGAG 
AAGCTCTGCA AGAATCCATG TGCGATGTTT GCTGCTGGAG AGATCAAAAC GCCGACAGGG 
GAGGGCCTTC TTGACTCACC CAGCAAAACC ATGTCTATTA AAGAAAGATT GGCACTGTTG 
AAGAAAAGCG GGGAGGAAGA TTGGAGAAAC AGACTCAGCA GGAGGCAGGA GGGCGGCAAG 
GCGCCGGCCA GCAGCCTGCA CACCCAGGAA GCAGGGCGGT CCCTCATCAA GAAGGAAGAA 
GGAGGAGTTG CGGATGATAG TGCCATTTCT AATCTGCTTT GGGAACCTGT ATATGCTTCT 
ACTTATTCTC CTGCTATTCC TGCTGCCCAT AAATACCTGT CTTTTGTGTC GATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAAATG ACGATTGAGG AGAGGAAGCA GCTCATCACT 
GTGAGAGAGG AGGCCTGGAA GACGAGAGGC AGAGGAGCGG CCAACGACTC GACCCAGTTC 
ACTGTGGCTG GCAGGATGGT GAAGAAAGGT TTGGCGTCAC CTACTGCCAT AACCCCAGTA 
GCCTCACCCA TTTGCGGTAA AACAAGAGGC ACCACACCCG TTTCCAAACC CCTGGAAGAT 
ATCGAAGCCA GACCAGATAT GCAGTTAGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGC GGGATGCACG AAACGGTGCT CACTGTCACC 
GGCAAATCTG TGAAGGAGGT GATGAAGCCA GATGATGATG AAACCTTTGC CAAATTTTAC 
CGCAGCGTGG ATTATAATAT GCCAAGAAGT CCTGTGGAGA TGGATGAGGA CTTCGATGTC 
ATTTTCGATC CTTATGCACC CAAATTGACG TCTTCCGTGG CCGAGCACAA GCGGGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCCTCCAAA AACCCCCTGA AAATGCTGGC GGCAAGAGAA 
GATCTCCTTC AGGAATACAC TGAGCAGAGA TTAAACGTTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC TTCCAACTCC AACTTCTCAG AAGTCACCCT GGCGGGTTTA 
GCCAGTAAAG AAAACTTCAG CAACGTCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAC 
TCTAACAACA GCGCCGTGCC CTACAAGAGG CTGATGCTGT TGCAGATTAA AGGAAGAAGA 
CATGTGCAGA CCAGGCTGGT GGAACCTCGA GCTTCGGCGC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CTCCCCACTG CTGCTTCCTG TGGGTAGGAG AGTTTGCAAA CGTCATAGAA 
AAGGCGAAGG CCTCAGAACT TGCAACTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACTTATA TCCAAACCAT TGAAGAAGGA ATTAATACAC ACACTCATGC AGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAATCTG CTGGAGACCC AAAAGAAGAT 
GAACTCTATG AAGCAGCCAT AATAGAAACT AACTGCATTT ACCGTCTCAT GGATGACAAA 
CTTGTTCCTG ATGACGACTA CTGGGGGAAA ATTCCGAAGT GCTCCCTTCT GCAACCCAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTTTACGTAT GGCATGGGAA AGAAGTCACA 
TTAGCACAAC GAAAAATAGC ATTTCAGCTG GCAAAGCACT TATGGAATGG AACCTTTGAC 
TATGAGAACT GTGACATCAA TCCCCTGGAT CCTGGAGAAT GCAATCCGCT TATCCCCAGA 
AAAGGACAGG GGCGGCCCGA CTGGGCGATA TTTGGGAGAC TTACTGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAGTT TCTGGATTGG ACGGAACTGA AGAGATCGAA TGAGAAGAAC 
CCCGGGGAAC TTGCCCAGCA CAAGGAAGAC CCCAGGACTG ATGTCAAGGC ATACGATGTG 
ACACGGATGG TGTCCATGCC CCAGACGACA GCAGGCACCA TCCTGGACGG AGTGAACGTC 
GGCCGTGGCT ATGGCCTGGT GGAAGGACAC GACAGGAGGC AGTTTGAGAT CACCAGCGTT 
TCCGTGGATG TCTGGCACAT CCTGGAATTC GACTATAGCA GGCTCCCCAA ACAAAGCATC 
GGGCAGTTCC ATGAGGGGGA TGCCTATGTG GTCAAGTGGA AGTTCATGGT GAGCACGGCA 
GTGGGAAGTC GCCAGAAGGG AGAGCACTCG GTGAGGGCAG CCGGCAAAGA GAAGTGCGTC 
TACTTCTTCT GGCAAGGCCG GCACTCCACC GTGAGTGAGA AGGGCACGTC GGCGCTGATG 
ACGGTGGAGC TGGACGAGGA AAGGGGGGCC CAGGTCCAGG TTCTCCAGGG AAAGGAGCCC 
CCCTGTTTCC TGCAGTGTTT CCAGGGGGGG ATGGTGGTGC ACTCGGGGAG GCGGGAAGAG 
GAAGAAGAAA ATGTGCAAAG TGAGTGGCGG CTGTACTGCG TGCGTGGAGA GGTGCCCGTG 
GAAGGGAATT TGCTGGAAGT GGCCTGTCAC TGTAGCAGCC TGAGGTCCAG AACTTCCATG 
GTGGTGCTTA ACGTCAACAA GGCCCTCATC TACCTGTGGC ACGGATGCAA AGCCCAGGCC 
CACACGAAGG AGGTCGGAAG GACCGCTGCG AACAAGATCA AGGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTCACA ATACACGAGT GTGATGAAGG CTCCGAGCCA 
CTCGGATTCT GGGATGCCTT AGGAAGGAGA GACAGGAAAG CCTACGATTG CATGCTTCAA 
GATCCTGGAA GTTTTAACTT CGCGCCCCGC CTGTTCATCC TCAGCAGCTC CTCTGGGGAT 
TTTGCAGCCA CAGAGTTTGT GTACCCTGCC CGAGCCCCCT CTGTGGTCAG TTCCATGCCC 
TTCCTGCAGG AAGATCTGTA CAGCGCGCCC CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTGT ACCTCTGGCA AGGCTGGTGG CCCATCGAGA ACAAGATCAC TGGTTCCGCC 
CGCATCCGCT GGGCCTCCGA CCGGAAGAGT GCGATGGAGA CTGTGCTCCA GTACTGCAAA 
GGAAAAAATC TCAAGAAACC AGCCCCCAAG TCTTACCTTA TCCACGCTGG TCTGGAGCCC 
CTGACATTCA CCAATATGTT TCCCAGCTGG GAGCACAGAG AGGACATCGC TGAGATCACA 
GAGATGGACA CGGAAGTTTC CAATCAGATC ACCCTCGTGG AAGACGTCTT AGCCAAGCTC 
TGTAAAACCA TTTACCCGCT GGCCGACCTC CTGGCCAGGC CACTCCCGGA GGGGGTCGAT 
CCTCTGAAGC TTGAGATCTA TCTCACCGAC GAAGACTTCG AGTTTGCACT AGACATGACG 
AGGGATGAAT ACAACGCCCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAAGGC 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448148.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303916.1 Homo sapiens supervillin (SVIL), transcript variant X16, mRNA.

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CloneID	OHu60828
Clone ID Related Accession (Same CDS sequence)	XM_024448148.1
Accession Version	XM_024448148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGGTG CACTTCACAT 
TCAGAAACGC CAACTGTCGA TGATGAAGAA AAGGTGGATG AACGAGCCAA GCTGAGCGTC 
GCCGCCAAGA GGTTGCTTTT CAGGGAGATG GAAAAATCTT TTGATGAACA AAATGTTCCA 
AAGCGACGCT CAAGAAACAC AGCTGTGGAG CAGAGGCTAC GCCGTCTGCA GGACAGGTCC 
CTCACCCAGC CCATCACCAC TGAAGAGGTG GTCATCGCAG CCACATTGCA GGCCTCTGCT 
CACCAAAAGG CCTTAGCCAA GGACCAGACA AATGAGGGCA AAGAGCTTGC TGAGCAAGGA 
GAACCTGATT CCTCCACTCT AAGCTTGGCC GAAAAGTTGG CCTTGTTTAA CAAATTGTCC 
CAGCCAGTCT CAAAAGCGAT TTCTACCCGG AACAGAATAG ACACGAGACA GAGGAGAATG 
AACGCTCGCT ATCAAACTCA GCCAGTCACA CTGGGAGAGG TGGAGCAGGT GCAGAGTGGA 
AAGCTCATTC CTTTCTCACC TGCCGTGAAC ACATCAGTGT CTACCGTAGC ATCCACGGTT 
GCTCCAATGT ATGCCGGAGA TCTTCGCACA AAGCCACCTC TTGACCACAA TGCAAGTGCC 
ACTGACTATA AGTTTTCTTC TTCAATAGAA AATTCGGACT CTCCAGTTAG AAGCATTCTG 
AAATCGCAAG CTTGGCAGCC TTTGGTAGAG GGTAGCGAGA ACAAGGGAAT GTTGAGAGAA 
TATGGAGAGA CAGAAAGCAA GAGAGCTTTG ACAGGTCGAG ACAGTGGGAT GGAGAAGTAT 
GGGTCCTTTG AGGAAGCAGA AGCATCCTAC CCCATCCTGA ACCGAGCCAG GGAAGGAGAC 
AGCCATAAGG AATCTAAATA TGCTGTTCCC AGAAGAGGAA GCCTGGAACG GGCGAACCCT 
CCCATCACCC ACCTCGGGGA TGAACCGAAG GAATTTTCCA TGGCTAAAAT GAATGCACAA 
GGAAACTTGG ACTTGAGGGA CAGGCTGCCC TTTGAAGAGA AGGTGGAGGT GGAGAATGTT 
ATGAAAAGGA AGTTTTCACT AAGAGCGGCA GAGTTCGGGG AGCCCACTTC CGAGCAGACG 
GGGACAGCTG CTGGGAAAAC TATTGCTCAA ACCACAGCCC CCGTGTCCTG GAAGCCCCAG 
GATTCTTCGG AACAGCCACA GGAGAAGCTC TGCAAGAATC CATGTGCGAT GTTTGCTGCT 
GGAGAGATCA AAACGCCGAC AGGGGAGGGC CTTCTTGACT CACCCAGCAA AACCATGTCT 
ATTAAAGAAA GATTGGCACT GTTGAAGAAA AGCGGGGAGG AAGATTGGAG AAACAGACTC 
AGCAGGAGGC AGGAGGGCGG CAAGGCGCCG GCCAGCAGCC TGCACACCCA GGAAGCAGGG 
CGGTCCCTCA TCAAGAAGGA AGAAGGAGGA GTTGCGGATG ATAGTGCCAT TTCTAATCTG 
CTTTGGGAAC CTGTATATGC TTCTACTTAT TCTCCTGCTA TTCCTGCTGC CCATAAATAC 
CTGTCTTTTG TGTCGATTAA TCAGCGGGTC ACAGAAAGTC GAGAGAGCCA AATGACGATT 
GAGGAGAGGA AGCAGCTCAT CACTGTGAGA GAGGAGGCCT GGAAGACGAG AGGCAGAGGA 
GCGGCCAACG ACTCGACCCA GTTCACTGTG GCTGGCAGGA TGGTGAAGAA AGGTTTGGCG 
TCACCTACTG CCATAACCCC AGTAGCCTCA CCCATTTGCG GTAAAACAAG AGGCACCACA 
CCCGTTTCCA AACCCCTGGA AGATATCGAA GCCAGACCAG ATATGCAGTT AGAATCGGAC 
CTGAAGTTGG ACAGGCTGGA AACCTTTCTA AGAAGGCTGA ATAACAAAGT TGGCGGGATG 
CACGAAACGG TGCTCACTGT CACCGGCAAA TCTGTGAAGG AGGTGATGAA GCCAGATGAT 
GATGAAACCT TTGCCAAATT TTACCGCAGC GTGGATTATA ATATGCCAAG AAGTCCTGTG 
GAGATGGATG AGGACTTCGA TGTCATTTTC GATCCTTATG CACCCAAATT GACGTCTTCC 
GTGGCCGAGC ACAAGCGGGC AGTTAGGCCC AAGCGCCGGG TTCAGGCCTC CAAAAACCCC 
CTGAAAATGC TGGCGGCAAG AGAAGATCTC CTTCAGGAAT ACACTGAGCA GAGATTAAAC 
GTTGCCTTCA TGGAGTCAAA GCGGATGAAA GTAGAAAAGA TGTCTTCCAA CTCCAACTTC 
TCAGAAGTCA CCCTGGCGGG TTTAGCCAGT AAAGAAAACT TCAGCAACGT CAGCCTGCGG 
AGCGTCAACC TGACGGAACA GAACTCTAAC AACAGCGCCG TGCCCTACAA GAGGCTGATG 
CTGTTGCAGA TTAAAGGAAG AAGACATGTG CAGACCAGGC TGGTGGAACC TCGAGCTTCG 
GCGCTCAACA GTGGGGACTG CTTCCTCCTG CTCTCTCCCC ACTGCTGCTT CCTGTGGGTA 
GGAGAGTTTG CAAACGTCAT AGAAAAGGCG AAGGCCTCAG AACTTGCAAC TTTAATTCAG 
ACAAAGAGGG AACTTGGTTG TAGAGCTACT TATATCCAAA CCATTGAAGA AGGAATTAAT 
ACACACACTC ATGCAGCCAA AGACTTCTGG AAGCTTCTGG GTGGCCAAAC CAGTTACCAA 
TCTGCTGGAG ACCCAAAAGA AGATGAACTC TATGAAGCAG CCATAATAGA AACTAACTGC 
ATTTACCGTC TCATGGATGA CAAACTTGTT CCTGATGACG ACTACTGGGG GAAAATTCCG 
AAGTGCTCCC TTCTGCAACC CAAAGAGGTA CTGGTGTTTG ATTTTGGTAG TGAAGTTTAC 
GTATGGCATG GGAAAGAAGT CACATTAGCA CAACGAAAAA TAGCATTTCA GCTGGCAAAG 
CACTTATGGA ATGGAACCTT TGACTATGAG AACTGTGACA TCAATCCCCT GGATCCTGGA 
GAATGCAATC CGCTTATCCC CAGAAAAGGA CAGGGGCGGC CCGACTGGGC GATATTTGGG 
AGACTTACTG AACACAATGA GACGATTTTG TTCAAAGAGA AGTTTCTGGA TTGGACGGAA 
CTGAAGAGAT CGAATGAGAA GAACCCCGGG GAACTTGCCC AGCACAAGGA AGACCCCAGG 
ACTGATGTCA AGGCATACGA TGTGACACGG ATGGTGTCCA TGCCCCAGAC GACAGCAGGC 
ACCATCCTGG ACGGAGTGAA CGTCGGCCGT GGCTATGGCC TGGTGGAAGG ACACGACAGG 
AGGCAGTTTG AGATCACCAG CGTTTCCGTG GATGTCTGGC ACATCCTGGA ATTCGACTAT 
AGCAGGCTCC CCAAACAAAG CATCGGGCAG TTCCATGAGG GGGATGCCTA TGTGGTCAAG 
TGGAAGTTCA TGGTGAGCAC GGCAGTGGGA AGTCGCCAGA AGGGAGAGCA CTCGGTGAGG 
GCAGCCGGCA AAGAGAAGTG CGTCTACTTC TTCTGGCAAG GCCGGCACTC CACCGTGAGT 
GAGAAGGGCA CGTCGGCGCT GATGACGGTG GAGCTGGACG AGGAAAGGGG GGCCCAGGTC 
CAGGTTCTCC AGGGAAAGGA GCCCCCCTGT TTCCTGCAGT GTTTCCAGGG GGGGATGGTG 
GTGCACTCGG GGAGGCGGGA AGAGGAAGAA GAAAATGTGC AAAGTGAGTG GCGGCTGTAC 
TGCGTGCGTG GAGAGGTGCC CGTGGAAGGG AATTTGCTGG AAGTGGCCTG TCACTGTAGC 
AGCCTGAGGT CCAGAACTTC CATGGTGGTG CTTAACGTCA ACAAGGCCCT CATCTACCTG 
TGGCACGGAT GCAAAGCCCA GGCCCACACG AAGGAGGTCG GAAGGACCGC TGCGAACAAG 
ATCAAGGAAC AATGTCCCCT GGAAGCAGGA CTGCATAGTA GCAGCAAAGT CACAATACAC 
GAGTGTGATG AAGGCTCCGA GCCACTCGGA TTCTGGGATG CCTTAGGAAG GAGAGACAGG 
AAAGCCTACG ATTGCATGCT TCAAGATCCT GGAAGTTTTA ACTTCGCGCC CCGCCTGTTC 
ATCCTCAGCA GCTCCTCTGG GGATTTTGCA GCCACAGAGT TTGTGTACCC TGCCCGAGCC 
CCCTCTGTGG TCAGTTCCAT GCCCTTCCTG CAGGAAGATC TGTACAGCGC GCCCCAGCCA 
GCACTTTTCC TTGTTGACAA TCACCACGAG GTGTACCTCT GGCAAGGCTG GTGGCCCATC 
GAGAACAAGA TCACTGGTTC CGCCCGCATC CGCTGGGCCT CCGACCGGAA GAGTGCGATG 
GAGACTGTGC TCCAGTACTG CAAAGGAAAA AATCTCAAGA AACCAGCCCC CAAGTCTTAC 
CTTATCCACG CTGGTCTGGA GCCCCTGACA TTCACCAATA TGTTTCCCAG CTGGGAGCAC 
AGAGAGGACA TCGCTGAGAT CACAGAGATG GACACGGAAG TTTCCAATCA GATCACCCTC 
GTGGAAGACG TCTTAGCCAA GCTCTGTAAA ACCATTTACC CGCTGGCCGA CCTCCTGGCC 
AGGCCACTCC CGGAGGGGGT CGATCCTCTG AAGCTTGAGA TCTATCTCAC CGACGAAGAC 
TTCGAGTTTG CACTAGACAT GACGAGGGAT GAATACAACG CCCTGCCCGC CTGGAAGCAG 
GTGAACCTGA AGAAAGCAAA AGGCCTGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303916.1
CDS	201..5693
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448148.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448148.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGGTG CACTTCACAT 
TCAGAAACGC CAACTGTCGA TGATGAAGAA AAGGTGGATG AACGAGCCAA GCTGAGCGTC 
GCCGCCAAGA GGTTGCTTTT CAGGGAGATG GAAAAATCTT TTGATGAACA AAATGTTCCA 
AAGCGACGCT CAAGAAACAC AGCTGTGGAG CAGAGGCTAC GCCGTCTGCA GGACAGGTCC 
CTCACCCAGC CCATCACCAC TGAAGAGGTG GTCATCGCAG CCACATTGCA GGCCTCTGCT 
CACCAAAAGG CCTTAGCCAA GGACCAGACA AATGAGGGCA AAGAGCTTGC TGAGCAAGGA 
GAACCTGATT CCTCCACTCT AAGCTTGGCC GAAAAGTTGG CCTTGTTTAA CAAATTGTCC 
CAGCCAGTCT CAAAAGCGAT TTCTACCCGG AACAGAATAG ACACGAGACA GAGGAGAATG 
AACGCTCGCT ATCAAACTCA GCCAGTCACA CTGGGAGAGG TGGAGCAGGT GCAGAGTGGA 
AAGCTCATTC CTTTCTCACC TGCCGTGAAC ACATCAGTGT CTACCGTAGC ATCCACGGTT 
GCTCCAATGT ATGCCGGAGA TCTTCGCACA AAGCCACCTC TTGACCACAA TGCAAGTGCC 
ACTGACTATA AGTTTTCTTC TTCAATAGAA AATTCGGACT CTCCAGTTAG AAGCATTCTG 
AAATCGCAAG CTTGGCAGCC TTTGGTAGAG GGTAGCGAGA ACAAGGGAAT GTTGAGAGAA 
TATGGAGAGA CAGAAAGCAA GAGAGCTTTG ACAGGTCGAG ACAGTGGGAT GGAGAAGTAT 
GGGTCCTTTG AGGAAGCAGA AGCATCCTAC CCCATCCTGA ACCGAGCCAG GGAAGGAGAC 
AGCCATAAGG AATCTAAATA TGCTGTTCCC AGAAGAGGAA GCCTGGAACG GGCGAACCCT 
CCCATCACCC ACCTCGGGGA TGAACCGAAG GAATTTTCCA TGGCTAAAAT GAATGCACAA 
GGAAACTTGG ACTTGAGGGA CAGGCTGCCC TTTGAAGAGA AGGTGGAGGT GGAGAATGTT 
ATGAAAAGGA AGTTTTCACT AAGAGCGGCA GAGTTCGGGG AGCCCACTTC CGAGCAGACG 
GGGACAGCTG CTGGGAAAAC TATTGCTCAA ACCACAGCCC CCGTGTCCTG GAAGCCCCAG 
GATTCTTCGG AACAGCCACA GGAGAAGCTC TGCAAGAATC CATGTGCGAT GTTTGCTGCT 
GGAGAGATCA AAACGCCGAC AGGGGAGGGC CTTCTTGACT CACCCAGCAA AACCATGTCT 
ATTAAAGAAA GATTGGCACT GTTGAAGAAA AGCGGGGAGG AAGATTGGAG AAACAGACTC 
AGCAGGAGGC AGGAGGGCGG CAAGGCGCCG GCCAGCAGCC TGCACACCCA GGAAGCAGGG 
CGGTCCCTCA TCAAGAAGGA AGAAGGAGGA GTTGCGGATG ATAGTGCCAT TTCTAATCTG 
CTTTGGGAAC CTGTATATGC TTCTACTTAT TCTCCTGCTA TTCCTGCTGC CCATAAATAC 
CTGTCTTTTG TGTCGATTAA TCAGCGGGTC ACAGAAAGTC GAGAGAGCCA AATGACGATT 
GAGGAGAGGA AGCAGCTCAT CACTGTGAGA GAGGAGGCCT GGAAGACGAG AGGCAGAGGA 
GCGGCCAACG ACTCGACCCA GTTCACTGTG GCTGGCAGGA TGGTGAAGAA AGGTTTGGCG 
TCACCTACTG CCATAACCCC AGTAGCCTCA CCCATTTGCG GTAAAACAAG AGGCACCACA 
CCCGTTTCCA AACCCCTGGA AGATATCGAA GCCAGACCAG ATATGCAGTT AGAATCGGAC 
CTGAAGTTGG ACAGGCTGGA AACCTTTCTA AGAAGGCTGA ATAACAAAGT TGGCGGGATG 
CACGAAACGG TGCTCACTGT CACCGGCAAA TCTGTGAAGG AGGTGATGAA GCCAGATGAT 
GATGAAACCT TTGCCAAATT TTACCGCAGC GTGGATTATA ATATGCCAAG AAGTCCTGTG 
GAGATGGATG AGGACTTCGA TGTCATTTTC GATCCTTATG CACCCAAATT GACGTCTTCC 
GTGGCCGAGC ACAAGCGGGC AGTTAGGCCC AAGCGCCGGG TTCAGGCCTC CAAAAACCCC 
CTGAAAATGC TGGCGGCAAG AGAAGATCTC CTTCAGGAAT ACACTGAGCA GAGATTAAAC 
GTTGCCTTCA TGGAGTCAAA GCGGATGAAA GTAGAAAAGA TGTCTTCCAA CTCCAACTTC 
TCAGAAGTCA CCCTGGCGGG TTTAGCCAGT AAAGAAAACT TCAGCAACGT CAGCCTGCGG 
AGCGTCAACC TGACGGAACA GAACTCTAAC AACAGCGCCG TGCCCTACAA GAGGCTGATG 
CTGTTGCAGA TTAAAGGAAG AAGACATGTG CAGACCAGGC TGGTGGAACC TCGAGCTTCG 
GCGCTCAACA GTGGGGACTG CTTCCTCCTG CTCTCTCCCC ACTGCTGCTT CCTGTGGGTA 
GGAGAGTTTG CAAACGTCAT AGAAAAGGCG AAGGCCTCAG AACTTGCAAC TTTAATTCAG 
ACAAAGAGGG AACTTGGTTG TAGAGCTACT TATATCCAAA CCATTGAAGA AGGAATTAAT 
ACACACACTC ATGCAGCCAA AGACTTCTGG AAGCTTCTGG GTGGCCAAAC CAGTTACCAA 
TCTGCTGGAG ACCCAAAAGA AGATGAACTC TATGAAGCAG CCATAATAGA AACTAACTGC 
ATTTACCGTC TCATGGATGA CAAACTTGTT CCTGATGACG ACTACTGGGG GAAAATTCCG 
AAGTGCTCCC TTCTGCAACC CAAAGAGGTA CTGGTGTTTG ATTTTGGTAG TGAAGTTTAC 
GTATGGCATG GGAAAGAAGT CACATTAGCA CAACGAAAAA TAGCATTTCA GCTGGCAAAG 
CACTTATGGA ATGGAACCTT TGACTATGAG AACTGTGACA TCAATCCCCT GGATCCTGGA 
GAATGCAATC CGCTTATCCC CAGAAAAGGA CAGGGGCGGC CCGACTGGGC GATATTTGGG 
AGACTTACTG AACACAATGA GACGATTTTG TTCAAAGAGA AGTTTCTGGA TTGGACGGAA 
CTGAAGAGAT CGAATGAGAA GAACCCCGGG GAACTTGCCC AGCACAAGGA AGACCCCAGG 
ACTGATGTCA AGGCATACGA TGTGACACGG ATGGTGTCCA TGCCCCAGAC GACAGCAGGC 
ACCATCCTGG ACGGAGTGAA CGTCGGCCGT GGCTATGGCC TGGTGGAAGG ACACGACAGG 
AGGCAGTTTG AGATCACCAG CGTTTCCGTG GATGTCTGGC ACATCCTGGA ATTCGACTAT 
AGCAGGCTCC CCAAACAAAG CATCGGGCAG TTCCATGAGG GGGATGCCTA TGTGGTCAAG 
TGGAAGTTCA TGGTGAGCAC GGCAGTGGGA AGTCGCCAGA AGGGAGAGCA CTCGGTGAGG 
GCAGCCGGCA AAGAGAAGTG CGTCTACTTC TTCTGGCAAG GCCGGCACTC CACCGTGAGT 
GAGAAGGGCA CGTCGGCGCT GATGACGGTG GAGCTGGACG AGGAAAGGGG GGCCCAGGTC 
CAGGTTCTCC AGGGAAAGGA GCCCCCCTGT TTCCTGCAGT GTTTCCAGGG GGGGATGGTG 
GTGCACTCGG GGAGGCGGGA AGAGGAAGAA GAAAATGTGC AAAGTGAGTG GCGGCTGTAC 
TGCGTGCGTG GAGAGGTGCC CGTGGAAGGG AATTTGCTGG AAGTGGCCTG TCACTGTAGC 
AGCCTGAGGT CCAGAACTTC CATGGTGGTG CTTAACGTCA ACAAGGCCCT CATCTACCTG 
TGGCACGGAT GCAAAGCCCA GGCCCACACG AAGGAGGTCG GAAGGACCGC TGCGAACAAG 
ATCAAGGAAC AATGTCCCCT GGAAGCAGGA CTGCATAGTA GCAGCAAAGT CACAATACAC 
GAGTGTGATG AAGGCTCCGA GCCACTCGGA TTCTGGGATG CCTTAGGAAG GAGAGACAGG 
AAAGCCTACG ATTGCATGCT TCAAGATCCT GGAAGTTTTA ACTTCGCGCC CCGCCTGTTC 
ATCCTCAGCA GCTCCTCTGG GGATTTTGCA GCCACAGAGT TTGTGTACCC TGCCCGAGCC 
CCCTCTGTGG TCAGTTCCAT GCCCTTCCTG CAGGAAGATC TGTACAGCGC GCCCCAGCCA 
GCACTTTTCC TTGTTGACAA TCACCACGAG GTGTACCTCT GGCAAGGCTG GTGGCCCATC 
GAGAACAAGA TCACTGGTTC CGCCCGCATC CGCTGGGCCT CCGACCGGAA GAGTGCGATG 
GAGACTGTGC TCCAGTACTG CAAAGGAAAA AATCTCAAGA AACCAGCCCC CAAGTCTTAC 
CTTATCCACG CTGGTCTGGA GCCCCTGACA TTCACCAATA TGTTTCCCAG CTGGGAGCAC 
AGAGAGGACA TCGCTGAGAT CACAGAGATG GACACGGAAG TTTCCAATCA GATCACCCTC 
GTGGAAGACG TCTTAGCCAA GCTCTGTAAA ACCATTTACC CGCTGGCCGA CCTCCTGGCC 
AGGCCACTCC CGGAGGGGGT CGATCCTCTG AAGCTTGAGA TCTATCTCAC CGACGAAGAC 
TTCGAGTTTG CACTAGACAT GACGAGGGAT GAATACAACG CCCTGCCCGC CTGGAAGCAG 
GTGAACCTGA AGAAAGCAAA AGGCCTGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_017016578.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872067.1 Homo sapiens supervillin (SVIL), transcript variant X14, mRNA.

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CloneID	OHu91605
Clone ID Related Accession (Same CDS sequence)	XM_017016578.1
Accession Version	XM_017016578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGCAAATCA CGGGTGGAGA GGTCGGCTGA AGGACCTGGC 
TTGCCCACCG GTGTGGAACG GGAGAGAGGG TCCCGGAAAC CAAGACGCTA TTTTTCTCCT 
GGTGAAAGTA GAAAAACTTC CGAGAGATTT AGAACCCAAC CTATAACTTC AGCAGAACGA 
AAGGAATCGG ATAGGTGCAC TTCACATTCA GAAACGCCAA CTGTCGATGA TGAAGAAAAG 
GTGGATGAAC GAGCCAAGCT GAGCGTCGCC GCCAAGAGGT TGCTTTTCAG GGAGATGGAA 
AAATCTTTTG ATGAACAAAA TGTTCCAAAG CGACGCTCAA GAAACACAGC TGTGGAGCAG 
AGGCTACGCC GTCTGCAGGA CAGGTCCCTC ACCCAGCCCA TCACCACTGA AGAGGTGGTC 
ATCGCAGCCA CATTGCAGGC CTCTGCTCAC CAAAAGGCCT TAGCCAAGGA CCAGACAAAT 
GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA CCTGATTCCT CCACTCTAAG CTTGGCCGAA 
AAGTTGGCCT TGTTTAACAA ATTGTCCCAG CCAGTCTCAA AAGCGATTTC TACCCGGAAC 
AGAATAGACA CGAGACAGAG GAGAATGAAC GCTCGCTATC AAACTCAGCC AGTCACACTG 
GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG CTCATTCCTT TCTCACCTGC CGTGAACACA 
TCAGTGTCTA CCGTAGCATC CACGGTTGCT CCAATGTATG CCGGAGATCT TCGCACAAAG 
CCACCTCTTG ACCACAATGC AAGTGCCACT GACTATAAGT TTTCTTCTTC AATAGAAAAT 
TCGGACTCTC CAGTTAGAAG CATTCTGAAA TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT 
AGCGAGAACA AGGGAATGTT GAGAGAATAT GGAGAGACAG AAAGCAAGAG AGCTTTGACA 
GGTCGAGACA GTGGGATGGA GAAGTATGGG TCCTTTGAGG AAGCAGAAGC ATCCTACCCC 
ATCCTGAACC GAGCCAGGGA AGGAGACAGC CATAAGGAAT CTAAATATGC TGTTCCCAGA 
AGAGGAAGCC TGGAACGGGC GAACCCTCCC ATCACCCACC TCGGGGATGA ACCGAAGGAA 
TTTTCCATGG CTAAAATGAA TGCACAAGGA AACTTGGACT TGAGGGACAG GCTGCCCTTT 
GAAGAGAAGG TGGAGGTGGA GAATGTTATG AAAAGGAAGT TTTCACTAAG AGCGGCAGAG 
TTCGGGGAGC CCACTTCCGA GCAGACGGGG ACAGCTGCTG GGAAAACTAT TGCTCAAACC 
ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT TCTTCGGAAC AGCCACAGGA GAAGCTCTGC 
AAGAATCCAT GTGCGATGTT TGCTGCTGGA GAGATCAAAA CGCCGACAGG GGAGGGCCTT 
CTTGACTCAC CCAGCAAAAC CATGTCTATT AAAGAAAGAT TGGCACTGTT GAAGAAAAGC 
GGGGAGGAAG ATTGGAGAAA CAGACTCAGC AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC 
AGCAGCCTGC ACACCCAGGA AGCAGGGCGG TCCCTCATCA AGAAGGAAGA AGGAGGAGTT 
GCGGATGATA GTGCCATTTC TAATCTGCTT TGGGAACCTG TATATGCTTC TACTTATTCT 
CCTGCTATTC CTGCTGCCCA TAAATACCTG TCTTTTGTGT CGATTAATCA GCGGGTCACA 
GAAAGTCGAG AGAGCCAAAT GACGATTGAG GAGAGGAAGC AGCTCATCAC TGTGAGAGAG 
GAGGCCTGGA AGACGAGAGG CAGAGGAGCG GCCAACGACT CGACCCAGTT CACTGTGGCT 
GGCAGGATGG TGAAGAAAGG TTTGGCGTCA CCTACTGCCA TAACCCCAGT AGCCTCACCC 
ATTTGCGGTA AAACAAGAGG CACCACACCC GTTTCCAAAC CCCTGGAAGA TATCGAAGCC 
AGACCAGATA TGCAGTTAGA ATCGGACCTG AAGTTGGACA GGCTGGAAAC CTTTCTAAGA 
AGGCTGAATA ACAAAGTTGG CGGGATGCAC GAAACGGTGC TCACTGTCAC CGGCAAATCT 
GTGAAGGAGG TGATGAAGCC AGATGATGAT GAAACCTTTG CCAAATTTTA CCGCAGCGTG 
GATTATAATA TGCCAAGAAG TCCTGTGGAG ATGGATGAGG ACTTCGATGT CATTTTCGAT 
CCTTATGCAC CCAAATTGAC GTCTTCCGTG GCCGAGCACA AGCGGGCAGT TAGGCCCAAG 
CGCCGGGTTC AGGCCTCCAA AAACCCCCTG AAAATGCTGG CGGCAAGAGA AGATCTCCTT 
CAGGAATACA CTGAGCAGAG ATTAAACGTT GCCTTCATGG AGTCAAAGCG GATGAAAGTA 
GAAAAGATGT CTTCCAACTC CAACTTCTCA GAAGTCACCC TGGCGGGTTT AGCCAGTAAA 
GAAAACTTCA GCAACGTCAG CCTGCGGAGC GTCAACCTGA CGGAACAGAA CTCTAACAAC 
AGCGCCGTGC CCTACAAGAG GCTGATGCTG TTGCAGATTA AAGGAAGAAG ACATGTGCAG 
ACCAGGCTGG TGGAACCTCG AGCTTCGGCG CTCAACAGTG GGGACTGCTT CCTCCTGCTC 
TCTCCCCACT GCTGCTTCCT GTGGGTAGGA GAGTTTGCAA ACGTCATAGA AAAGGCGAAG 
GCCTCAGAAC TTGCAACTTT AATTCAGACA AAGAGGGAAC TTGGTTGTAG AGCTACTTAT 
ATCCAAACCA TTGAAGAAGG AATTAATACA CACACTCATG CAGCCAAAGA CTTCTGGAAG 
CTTCTGGGTG GCCAAACCAG TTACCAATCT GCTGGAGACC CAAAAGAAGA TGAACTCTAT 
GAAGCAGCCA TAATAGAAAC TAACTGCATT TACCGTCTCA TGGATGACAA ACTTGTTCCT 
GATGACGACT ACTGGGGGAA AATTCCGAAG TGCTCCCTTC TGCAACCCAA AGAGGTACTG 
GTGTTTGATT TTGGTAGTGA AGTTTACGTA TGGCATGGGA AAGAAGTCAC ATTAGCACAA 
CGAAAAATAG CATTTCAGCT GGCAAAGCAC TTATGGAATG GAACCTTTGA CTATGAGAAC 
TGTGACATCA ATCCCCTGGA TCCTGGAGAA TGCAATCCGC TTATCCCCAG AAAAGGACAG 
GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA CTTACTGAAC ACAATGAGAC GATTTTGTTC 
AAAGAGAAGT TTCTGGATTG GACGGAACTG AAGAGATCGA ATGAGAAGAA CCCCGGGGAA 
CTTGCCCAGC ACAAGGAAGA CCCCAGGACT GATGTCAAGG CATACGATGT GACACGGATG 
GTGTCCATGC CCCAGACGAC AGCAGGCACC ATCCTGGACG GAGTGAACGT CGGCCGTGGC 
TATGGCCTGG TGGAAGGACA CGACAGGAGG CAGTTTGAGA TCACCAGCGT TTCCGTGGAT 
GTCTGGCACA TCCTGGAATT CGACTATAGC AGGCTCCCCA AACAAAGCAT CGGGCAGTTC 
CATGAGGGGG ATGCCTATGT GGTCAAGTGG AAGTTCATGG TGAGCACGGC AGTGGGAAGT 
CGCCAGAAGG GAGAGCACTC GGTGAGGGCA GCCGGCAAAG AGAAGTGCGT CTACTTCTTC 
TGGCAAGGCC GGCACTCCAC CGTGAGTGAG AAGGGCACGT CGGCGCTGAT GACGGTGGAG 
CTGGACGAGG AAAGGGGGGC CCAGGTCCAG GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC 
CTGCAGTGTT TCCAGGGGGG GATGGTGGTG CACTCGGGGA GGCGGGAAGA GGAAGAAGAA 
AATGTGCAAA GTGAGTGGCG GCTGTACTGC GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT 
TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC CTGAGGTCCA GAACTTCCAT GGTGGTGCTT 
AACGTCAACA AGGCCCTCAT CTACCTGTGG CACGGATGCA AAGCCCAGGC CCACACGAAG 
GAGGTCGGAA GGACCGCTGC GAACAAGATC AAGGAACAAT GTCCCCTGGA AGCAGGACTG 
CATAGTAGCA GCAAAGTCAC AATACACGAG TGTGATGAAG GCTCCGAGCC ACTCGGATTC 
TGGGATGCCT TAGGAAGGAG AGACAGGAAA GCCTACGATT GCATGCTTCA AGATCCTGGA 
AGTTTTAACT TCGCGCCCCG CCTGTTCATC CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC 
ACAGAGTTTG TGTACCCTGC CCGAGCCCCC TCTGTGGTCA GTTCCATGCC CTTCCTGCAG 
GAAGATCTGT ACAGCGCGCC CCAGCCAGCA CTTTTCCTTG TTGACAATCA CCACGAGGTG 
TACCTCTGGC AAGGCTGGTG GCCCATCGAG AACAAGATCA CTGGTTCCGC CCGCATCCGC 
TGGGCCTCCG ACCGGAAGAG TGCGATGGAG ACTGTGCTCC AGTACTGCAA AGGAAAAAAT 
CTCAAGAAAC CAGCCCCCAA GTCTTACCTT ATCCACGCTG GTCTGGAGCC CCTGACATTC 
ACCAATATGT TTCCCAGCTG GGAGCACAGA GAGGACATCG CTGAGATCAC AGAGATGGAC 
ACGGAAGTTT CCAATCAGAT CACCCTCGTG GAAGACGTCT TAGCCAAGCT CTGTAAAACC 
ATTTACCCGC TGGCCGACCT CCTGGCCAGG CCACTCCCGG AGGGGGTCGA TCCTCTGAAG 
CTTGAGATCT ATCTCACCGA CGAAGACTTC GAGTTTGCAC TAGACATGAC GAGGGATGAA 
TACAACGCCC TGCCCGCCTG GAAGCAGGTG AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872067.1
CDS	1241..7240
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSSKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_017016578.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016578.1

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGCAAATCA CGGGTGGAGA GGTCGGCTGA AGGACCTGGC 
TTGCCCACCG GTGTGGAACG GGAGAGAGGG TCCCGGAAAC CAAGACGCTA TTTTTCTCCT 
GGTGAAAGTA GAAAAACTTC CGAGAGATTT AGAACCCAAC CTATAACTTC AGCAGAACGA 
AAGGAATCGG ATAGGTGCAC TTCACATTCA GAAACGCCAA CTGTCGATGA TGAAGAAAAG 
GTGGATGAAC GAGCCAAGCT GAGCGTCGCC GCCAAGAGGT TGCTTTTCAG GGAGATGGAA 
AAATCTTTTG ATGAACAAAA TGTTCCAAAG CGACGCTCAA GAAACACAGC TGTGGAGCAG 
AGGCTACGCC GTCTGCAGGA CAGGTCCCTC ACCCAGCCCA TCACCACTGA AGAGGTGGTC 
ATCGCAGCCA CATTGCAGGC CTCTGCTCAC CAAAAGGCCT TAGCCAAGGA CCAGACAAAT 
GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA CCTGATTCCT CCACTCTAAG CTTGGCCGAA 
AAGTTGGCCT TGTTTAACAA ATTGTCCCAG CCAGTCTCAA AAGCGATTTC TACCCGGAAC 
AGAATAGACA CGAGACAGAG GAGAATGAAC GCTCGCTATC AAACTCAGCC AGTCACACTG 
GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG CTCATTCCTT TCTCACCTGC CGTGAACACA 
TCAGTGTCTA CCGTAGCATC CACGGTTGCT CCAATGTATG CCGGAGATCT TCGCACAAAG 
CCACCTCTTG ACCACAATGC AAGTGCCACT GACTATAAGT TTTCTTCTTC AATAGAAAAT 
TCGGACTCTC CAGTTAGAAG CATTCTGAAA TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT 
AGCGAGAACA AGGGAATGTT GAGAGAATAT GGAGAGACAG AAAGCAAGAG AGCTTTGACA 
GGTCGAGACA GTGGGATGGA GAAGTATGGG TCCTTTGAGG AAGCAGAAGC ATCCTACCCC 
ATCCTGAACC GAGCCAGGGA AGGAGACAGC CATAAGGAAT CTAAATATGC TGTTCCCAGA 
AGAGGAAGCC TGGAACGGGC GAACCCTCCC ATCACCCACC TCGGGGATGA ACCGAAGGAA 
TTTTCCATGG CTAAAATGAA TGCACAAGGA AACTTGGACT TGAGGGACAG GCTGCCCTTT 
GAAGAGAAGG TGGAGGTGGA GAATGTTATG AAAAGGAAGT TTTCACTAAG AGCGGCAGAG 
TTCGGGGAGC CCACTTCCGA GCAGACGGGG ACAGCTGCTG GGAAAACTAT TGCTCAAACC 
ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT TCTTCGGAAC AGCCACAGGA GAAGCTCTGC 
AAGAATCCAT GTGCGATGTT TGCTGCTGGA GAGATCAAAA CGCCGACAGG GGAGGGCCTT 
CTTGACTCAC CCAGCAAAAC CATGTCTATT AAAGAAAGAT TGGCACTGTT GAAGAAAAGC 
GGGGAGGAAG ATTGGAGAAA CAGACTCAGC AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC 
AGCAGCCTGC ACACCCAGGA AGCAGGGCGG TCCCTCATCA AGAAGGAAGA AGGAGGAGTT 
GCGGATGATA GTGCCATTTC TAATCTGCTT TGGGAACCTG TATATGCTTC TACTTATTCT 
CCTGCTATTC CTGCTGCCCA TAAATACCTG TCTTTTGTGT CGATTAATCA GCGGGTCACA 
GAAAGTCGAG AGAGCCAAAT GACGATTGAG GAGAGGAAGC AGCTCATCAC TGTGAGAGAG 
GAGGCCTGGA AGACGAGAGG CAGAGGAGCG GCCAACGACT CGACCCAGTT CACTGTGGCT 
GGCAGGATGG TGAAGAAAGG TTTGGCGTCA CCTACTGCCA TAACCCCAGT AGCCTCACCC 
ATTTGCGGTA AAACAAGAGG CACCACACCC GTTTCCAAAC CCCTGGAAGA TATCGAAGCC 
AGACCAGATA TGCAGTTAGA ATCGGACCTG AAGTTGGACA GGCTGGAAAC CTTTCTAAGA 
AGGCTGAATA ACAAAGTTGG CGGGATGCAC GAAACGGTGC TCACTGTCAC CGGCAAATCT 
GTGAAGGAGG TGATGAAGCC AGATGATGAT GAAACCTTTG CCAAATTTTA CCGCAGCGTG 
GATTATAATA TGCCAAGAAG TCCTGTGGAG ATGGATGAGG ACTTCGATGT CATTTTCGAT 
CCTTATGCAC CCAAATTGAC GTCTTCCGTG GCCGAGCACA AGCGGGCAGT TAGGCCCAAG 
CGCCGGGTTC AGGCCTCCAA AAACCCCCTG AAAATGCTGG CGGCAAGAGA AGATCTCCTT 
CAGGAATACA CTGAGCAGAG ATTAAACGTT GCCTTCATGG AGTCAAAGCG GATGAAAGTA 
GAAAAGATGT CTTCCAACTC CAACTTCTCA GAAGTCACCC TGGCGGGTTT AGCCAGTAAA 
GAAAACTTCA GCAACGTCAG CCTGCGGAGC GTCAACCTGA CGGAACAGAA CTCTAACAAC 
AGCGCCGTGC CCTACAAGAG GCTGATGCTG TTGCAGATTA AAGGAAGAAG ACATGTGCAG 
ACCAGGCTGG TGGAACCTCG AGCTTCGGCG CTCAACAGTG GGGACTGCTT CCTCCTGCTC 
TCTCCCCACT GCTGCTTCCT GTGGGTAGGA GAGTTTGCAA ACGTCATAGA AAAGGCGAAG 
GCCTCAGAAC TTGCAACTTT AATTCAGACA AAGAGGGAAC TTGGTTGTAG AGCTACTTAT 
ATCCAAACCA TTGAAGAAGG AATTAATACA CACACTCATG CAGCCAAAGA CTTCTGGAAG 
CTTCTGGGTG GCCAAACCAG TTACCAATCT GCTGGAGACC CAAAAGAAGA TGAACTCTAT 
GAAGCAGCCA TAATAGAAAC TAACTGCATT TACCGTCTCA TGGATGACAA ACTTGTTCCT 
GATGACGACT ACTGGGGGAA AATTCCGAAG TGCTCCCTTC TGCAACCCAA AGAGGTACTG 
GTGTTTGATT TTGGTAGTGA AGTTTACGTA TGGCATGGGA AAGAAGTCAC ATTAGCACAA 
CGAAAAATAG CATTTCAGCT GGCAAAGCAC TTATGGAATG GAACCTTTGA CTATGAGAAC 
TGTGACATCA ATCCCCTGGA TCCTGGAGAA TGCAATCCGC TTATCCCCAG AAAAGGACAG 
GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA CTTACTGAAC ACAATGAGAC GATTTTGTTC 
AAAGAGAAGT TTCTGGATTG GACGGAACTG AAGAGATCGA ATGAGAAGAA CCCCGGGGAA 
CTTGCCCAGC ACAAGGAAGA CCCCAGGACT GATGTCAAGG CATACGATGT GACACGGATG 
GTGTCCATGC CCCAGACGAC AGCAGGCACC ATCCTGGACG GAGTGAACGT CGGCCGTGGC 
TATGGCCTGG TGGAAGGACA CGACAGGAGG CAGTTTGAGA TCACCAGCGT TTCCGTGGAT 
GTCTGGCACA TCCTGGAATT CGACTATAGC AGGCTCCCCA AACAAAGCAT CGGGCAGTTC 
CATGAGGGGG ATGCCTATGT GGTCAAGTGG AAGTTCATGG TGAGCACGGC AGTGGGAAGT 
CGCCAGAAGG GAGAGCACTC GGTGAGGGCA GCCGGCAAAG AGAAGTGCGT CTACTTCTTC 
TGGCAAGGCC GGCACTCCAC CGTGAGTGAG AAGGGCACGT CGGCGCTGAT GACGGTGGAG 
CTGGACGAGG AAAGGGGGGC CCAGGTCCAG GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC 
CTGCAGTGTT TCCAGGGGGG GATGGTGGTG CACTCGGGGA GGCGGGAAGA GGAAGAAGAA 
AATGTGCAAA GTGAGTGGCG GCTGTACTGC GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT 
TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC CTGAGGTCCA GAACTTCCAT GGTGGTGCTT 
AACGTCAACA AGGCCCTCAT CTACCTGTGG CACGGATGCA AAGCCCAGGC CCACACGAAG 
GAGGTCGGAA GGACCGCTGC GAACAAGATC AAGGAACAAT GTCCCCTGGA AGCAGGACTG 
CATAGTAGCA GCAAAGTCAC AATACACGAG TGTGATGAAG GCTCCGAGCC ACTCGGATTC 
TGGGATGCCT TAGGAAGGAG AGACAGGAAA GCCTACGATT GCATGCTTCA AGATCCTGGA 
AGTTTTAACT TCGCGCCCCG CCTGTTCATC CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC 
ACAGAGTTTG TGTACCCTGC CCGAGCCCCC TCTGTGGTCA GTTCCATGCC CTTCCTGCAG 
GAAGATCTGT ACAGCGCGCC CCAGCCAGCA CTTTTCCTTG TTGACAATCA CCACGAGGTG 
TACCTCTGGC AAGGCTGGTG GCCCATCGAG AACAAGATCA CTGGTTCCGC CCGCATCCGC 
TGGGCCTCCG ACCGGAAGAG TGCGATGGAG ACTGTGCTCC AGTACTGCAA AGGAAAAAAT 
CTCAAGAAAC CAGCCCCCAA GTCTTACCTT ATCCACGCTG GTCTGGAGCC CCTGACATTC 
ACCAATATGT TTCCCAGCTG GGAGCACAGA GAGGACATCG CTGAGATCAC AGAGATGGAC 
ACGGAAGTTT CCAATCAGAT CACCCTCGTG GAAGACGTCT TAGCCAAGCT CTGTAAAACC 
ATTTACCCGC TGGCCGACCT CCTGGCCAGG CCACTCCCGG AGGGGGTCGA TCCTCTGAAG 
CTTGAGATCT ATCTCACCGA CGAAGACTTC GAGTTTGCAC TAGACATGAC GAGGGATGAA 
TACAACGCCC TGCCCGCCTG GAAGCAGGTG AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_017016579.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872068.1 Homo sapiens supervillin (SVIL), transcript variant X15, mRNA.

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CloneID	OHu91606
Clone ID Related Accession (Same CDS sequence)	XM_017016579.1
Accession Version	XM_017016579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGCAAATCA CGGGTGGAGA GGTCGGCTGA AGGACCTGGC 
TTGCCCACCG GTGTGGAACG GGAGAGAGGG TCCCGGAAAC CAAGACGCTA TTTTTCTCCT 
GGTGAAAGTA GAAAAACTTC CGAGAGATTT AGAACCCAAC CTATAACTTC AGCAGAACGA 
AAGGAATCGG ATAGGTGCAC TTCACATTCA GAAACGCCAA CTGTCGATGA TGAAGAAAAG 
GTGGATGAAC GAGCCAAGCT GAGCGTCGCC GCCAAGAGGT TGCTTTTCAG GGAGATGGAA 
AAATCTTTTG ATGAACAAAA TGTTCCAAAG CGACGCTCAA GAAACACAGC TGTGGAGCAG 
AGGCTACGCC GTCTGCAGGA CAGGTCCCTC ACCCAGCCCA TCACCACTGA AGAGGTGGTC 
ATCGCAGCCA CTGAACCTAT CCCCGCTTCG TGTTCTGGGG GCACCCACCC TGTAATGGCG 
AGACTTCCTA GCCCCACTGT AGCTAGGAGC GCTGTGCAGC CTGCCAGATT GCAGGCCTCT 
GCTCACCAAA AGGCCTTAGC CAAGGACCAG ACAAATGAGG GCAAAGAGCT TGCTGAGCAA 
GGAGAACCTG ATTCCTCCAC TCTAAGCTTG GCCGAAAAGT TGGCCTTGTT TAACAAATTG 
TCCCAGCCAG TCTCAAAAGC GATTTCTACC CGGAACAGAA TAGACACGAG ACAGAGGAGA 
ATGAACGCTC GCTATCAAAC TCAGCCAGTC ACACTGGGAG AGGTGGAGCA GGTGCAGAGT 
GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG AACACATCAG TGTCTACCGT AGCATCCACG 
GTTGCTCCAA TGTATGCCGG AGATCTTCGC ACAAAGCCAC CTCTTGACCA CAATGCAAGT 
GCCACTGACT ATAAGTTTTC TTCTTCAATA GAAAATTCGG ACTCTCCAGT TAGAAGCATT 
CTGAAATCGC AAGCTTGGCA GCCTTTGGTA GAGGGTAGCG AGAACAAGGG AATGTTGAGA 
GAATATGGAG AGACAGAAAG CAAGAGAGCT TTGACAGGTC GAGACAGTGG GATGGAGAAG 
TATGGGTCCT TTGAGGAAGC AGAAGCATCC TACCCCATCC TGAACCGAGC CAGGGAAGGA 
GACAGCCATA AGGAATCTAA ATATGCTGTT CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC 
CCTCCCATCA CCCACCTCGG GGATGAACCG AAGGAATTTT CCATGGCTAA AATGAATGCA 
CAAGGAAACT TGGACTTGAG GGACAGGCTG CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT 
GTTATGAAAA GGAAGTTTTC ACTAAGAGCG GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG 
ACGGGGACAG CTGCTGGGAA AACTATTGCT CAAACCACAG CCCCCGTGTC CTGGAAGCCC 
CAGGATTCTT CGGAACAGCC ACAGGAGAAG CTCTGCAAGA ATCCATGTGC GATGTTTGCT 
GCTGGAGAGA TCAAAACGCC GACAGGGGAG GGCCTTCTTG ACTCACCCAG CAAAACCATG 
TCTATTAAAG AAAGATTGGC ACTGTTGAAG AAAAGCGGGG AGGAAGATTG GAGAAACAGA 
CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG CCGGCCAGCA GCCTGCACAC CCAGGAAGCA 
GGGCGGTCCC TCATCAAGAA GCGGGTCACA GAAAGTCGAG AGAGCCAAAT GACGATTGAG 
GAGAGGAAGC AGCTCATCAC TGTGAGAGAG GAGGCCTGGA AGACGAGAGG CAGAGGAGCG 
GCCAACGACT CGACCCAGTT CACTGTGGCT GGCAGGATGG TGAAGAAAGG TTTGGCGTCA 
CCTACTGCCA TAACCCCAGT AGCCTCACCC ATTTGCGGTA AAACAAGAGG CACCACACCC 
GTTTCCAAAC CCCTGGAAGA TATCGAAGCC AGACCAGATA TGCAGTTAGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG CGGGATGCAC 
GAAACGGTGC TCACTGTCAC CGGCAAATCT GTGAAGGAGG TGATGAAGCC AGATGATGAT 
GAAACCTTTG CCAAATTTTA CCGCAGCGTG GATTATAATA TGCCAAGAAG TCCTGTGGAG 
ATGGATGAGG ACTTCGATGT CATTTTCGAT CCTTATGCAC CCAAATTGAC GTCTTCCGTG 
GCCGAGCACA AGCGGGCAGT TAGGCCCAAG CGCCGGGTTC AGGCCTCCAA AAACCCCCTG 
AAAATGCTGG CGGCAAGAGA AGATCTCCTT CAGGAATACA CTGAGCAGAG ATTAAACGTT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CTTCCAACTC CAACTTCTCA 
GAAGTCACCC TGGCGGGTTT AGCCAGTAAA GAAAACTTCA GCAACGTCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA CTCTAACAAC AGCGCCGTGC CCTACAAGAG GCTGATGCTG 
TTGCAGATTA AAGGAAGAAG ACATGTGCAG ACCAGGCTGG TGGAACCTCG AGCTTCGGCG 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCTCCCCACT GCTGCTTCCT GTGGGTAGGA 
GAGTTTGCAA ACGTCATAGA AAAGGCGAAG GCCTCAGAAC TTGCAACTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACTTAT ATCCAAACCA TTGAAGAAGG AATTAATACA 
CACACTCATG CAGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAATCT 
GCTGGAGACC CAAAAGAAGA TGAACTCTAT GAAGCAGCCA TAATAGAAAC TAACTGCATT 
TACCGTCTCA TGGATGACAA ACTTGTTCCT GATGACGACT ACTGGGGGAA AATTCCGAAG 
TGCTCCCTTC TGCAACCCAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTTTACGTA 
TGGCATGGGA AAGAAGTCAC ATTAGCACAA CGAAAAATAG CATTTCAGCT GGCAAAGCAC 
TTATGGAATG GAACCTTTGA CTATGAGAAC TGTGACATCA ATCCCCTGGA TCCTGGAGAA 
TGCAATCCGC TTATCCCCAG AAAAGGACAG GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA 
CTTACTGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAGT TTCTGGATTG GACGGAACTG 
AAGAGATCGA ATGAGAAGAA CCCCGGGGAA CTTGCCCAGC ACAAGGAAGA CCCCAGGACT 
GATGTCAAGG CATACGATGT GACACGGATG GTGTCCATGC CCCAGACGAC AGCAGGCACC 
ATCCTGGACG GAGTGAACGT CGGCCGTGGC TATGGCCTGG TGGAAGGACA CGACAGGAGG 
CAGTTTGAGA TCACCAGCGT TTCCGTGGAT GTCTGGCACA TCCTGGAATT CGACTATAGC 
AGGCTCCCCA AACAAAGCAT CGGGCAGTTC CATGAGGGGG ATGCCTATGT GGTCAAGTGG 
AAGTTCATGG TGAGCACGGC AGTGGGAAGT CGCCAGAAGG GAGAGCACTC GGTGAGGGCA 
GCCGGCAAAG AGAAGTGCGT CTACTTCTTC TGGCAAGGCC GGCACTCCAC CGTGAGTGAG 
AAGGGCACGT CGGCGCTGAT GACGGTGGAG CTGGACGAGG AAAGGGGGGC CCAGGTCCAG 
GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC CTGCAGTGTT TCCAGGGGGG GATGGTGGTG 
CACTCGGGGA GGCGGGAAGA GGAAGAAGAA AATGTGCAAA GTGAGTGGCG GCTGTACTGC 
GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC 
CTGAGGTCCA GAACTTCCAT GGTGGTGCTT AACGTCAACA AGGCCCTCAT CTACCTGTGG 
CACGGATGCA AAGCCCAGGC CCACACGAAG GAGGTCGGAA GGACCGCTGC GAACAAGATC 
AAGGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTCAC AATACACGAG 
TGTGATGAAG GCTCCGAGCC ACTCGGATTC TGGGATGCCT TAGGAAGGAG AGACAGGAAA 
GCCTACGATT GCATGCTTCA AGATCCTGGA AGTTTTAACT TCGCGCCCCG CCTGTTCATC 
CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC ACAGAGTTTG TGTACCCTGC CCGAGCCCCC 
TCTGTGGTCA GTTCCATGCC CTTCCTGCAG GAAGATCTGT ACAGCGCGCC CCAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTG TACCTCTGGC AAGGCTGGTG GCCCATCGAG 
AACAAGATCA CTGGTTCCGC CCGCATCCGC TGGGCCTCCG ACCGGAAGAG TGCGATGGAG 
ACTGTGCTCC AGTACTGCAA AGGAAAAAAT CTCAAGAAAC CAGCCCCCAA GTCTTACCTT 
ATCCACGCTG GTCTGGAGCC CCTGACATTC ACCAATATGT TTCCCAGCTG GGAGCACAGA 
GAGGACATCG CTGAGATCAC AGAGATGGAC ACGGAAGTTT CCAATCAGAT CACCCTCGTG 
GAAGACGTCT TAGCCAAGCT CTGTAAAACC ATTTACCCGC TGGCCGACCT CCTGGCCAGG 
CCACTCCCGG AGGGGGTCGA TCCTCTGAAG CTTGAGATCT ATCTCACCGA CGAAGACTTC 
GAGTTTGCAC TAGACATGAC GAGGGATGAA TACAACGCCC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872068.1
CDS	1241..7210
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSSKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_017016579.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016579.1

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGCAAATCA CGGGTGGAGA GGTCGGCTGA AGGACCTGGC 
TTGCCCACCG GTGTGGAACG GGAGAGAGGG TCCCGGAAAC CAAGACGCTA TTTTTCTCCT 
GGTGAAAGTA GAAAAACTTC CGAGAGATTT AGAACCCAAC CTATAACTTC AGCAGAACGA 
AAGGAATCGG ATAGGTGCAC TTCACATTCA GAAACGCCAA CTGTCGATGA TGAAGAAAAG 
GTGGATGAAC GAGCCAAGCT GAGCGTCGCC GCCAAGAGGT TGCTTTTCAG GGAGATGGAA 
AAATCTTTTG ATGAACAAAA TGTTCCAAAG CGACGCTCAA GAAACACAGC TGTGGAGCAG 
AGGCTACGCC GTCTGCAGGA CAGGTCCCTC ACCCAGCCCA TCACCACTGA AGAGGTGGTC 
ATCGCAGCCA CTGAACCTAT CCCCGCTTCG TGTTCTGGGG GCACCCACCC TGTAATGGCG 
AGACTTCCTA GCCCCACTGT AGCTAGGAGC GCTGTGCAGC CTGCCAGATT GCAGGCCTCT 
GCTCACCAAA AGGCCTTAGC CAAGGACCAG ACAAATGAGG GCAAAGAGCT TGCTGAGCAA 
GGAGAACCTG ATTCCTCCAC TCTAAGCTTG GCCGAAAAGT TGGCCTTGTT TAACAAATTG 
TCCCAGCCAG TCTCAAAAGC GATTTCTACC CGGAACAGAA TAGACACGAG ACAGAGGAGA 
ATGAACGCTC GCTATCAAAC TCAGCCAGTC ACACTGGGAG AGGTGGAGCA GGTGCAGAGT 
GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG AACACATCAG TGTCTACCGT AGCATCCACG 
GTTGCTCCAA TGTATGCCGG AGATCTTCGC ACAAAGCCAC CTCTTGACCA CAATGCAAGT 
GCCACTGACT ATAAGTTTTC TTCTTCAATA GAAAATTCGG ACTCTCCAGT TAGAAGCATT 
CTGAAATCGC AAGCTTGGCA GCCTTTGGTA GAGGGTAGCG AGAACAAGGG AATGTTGAGA 
GAATATGGAG AGACAGAAAG CAAGAGAGCT TTGACAGGTC GAGACAGTGG GATGGAGAAG 
TATGGGTCCT TTGAGGAAGC AGAAGCATCC TACCCCATCC TGAACCGAGC CAGGGAAGGA 
GACAGCCATA AGGAATCTAA ATATGCTGTT CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC 
CCTCCCATCA CCCACCTCGG GGATGAACCG AAGGAATTTT CCATGGCTAA AATGAATGCA 
CAAGGAAACT TGGACTTGAG GGACAGGCTG CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT 
GTTATGAAAA GGAAGTTTTC ACTAAGAGCG GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG 
ACGGGGACAG CTGCTGGGAA AACTATTGCT CAAACCACAG CCCCCGTGTC CTGGAAGCCC 
CAGGATTCTT CGGAACAGCC ACAGGAGAAG CTCTGCAAGA ATCCATGTGC GATGTTTGCT 
GCTGGAGAGA TCAAAACGCC GACAGGGGAG GGCCTTCTTG ACTCACCCAG CAAAACCATG 
TCTATTAAAG AAAGATTGGC ACTGTTGAAG AAAAGCGGGG AGGAAGATTG GAGAAACAGA 
CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG CCGGCCAGCA GCCTGCACAC CCAGGAAGCA 
GGGCGGTCCC TCATCAAGAA GCGGGTCACA GAAAGTCGAG AGAGCCAAAT GACGATTGAG 
GAGAGGAAGC AGCTCATCAC TGTGAGAGAG GAGGCCTGGA AGACGAGAGG CAGAGGAGCG 
GCCAACGACT CGACCCAGTT CACTGTGGCT GGCAGGATGG TGAAGAAAGG TTTGGCGTCA 
CCTACTGCCA TAACCCCAGT AGCCTCACCC ATTTGCGGTA AAACAAGAGG CACCACACCC 
GTTTCCAAAC CCCTGGAAGA TATCGAAGCC AGACCAGATA TGCAGTTAGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG CGGGATGCAC 
GAAACGGTGC TCACTGTCAC CGGCAAATCT GTGAAGGAGG TGATGAAGCC AGATGATGAT 
GAAACCTTTG CCAAATTTTA CCGCAGCGTG GATTATAATA TGCCAAGAAG TCCTGTGGAG 
ATGGATGAGG ACTTCGATGT CATTTTCGAT CCTTATGCAC CCAAATTGAC GTCTTCCGTG 
GCCGAGCACA AGCGGGCAGT TAGGCCCAAG CGCCGGGTTC AGGCCTCCAA AAACCCCCTG 
AAAATGCTGG CGGCAAGAGA AGATCTCCTT CAGGAATACA CTGAGCAGAG ATTAAACGTT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CTTCCAACTC CAACTTCTCA 
GAAGTCACCC TGGCGGGTTT AGCCAGTAAA GAAAACTTCA GCAACGTCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA CTCTAACAAC AGCGCCGTGC CCTACAAGAG GCTGATGCTG 
TTGCAGATTA AAGGAAGAAG ACATGTGCAG ACCAGGCTGG TGGAACCTCG AGCTTCGGCG 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCTCCCCACT GCTGCTTCCT GTGGGTAGGA 
GAGTTTGCAA ACGTCATAGA AAAGGCGAAG GCCTCAGAAC TTGCAACTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACTTAT ATCCAAACCA TTGAAGAAGG AATTAATACA 
CACACTCATG CAGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAATCT 
GCTGGAGACC CAAAAGAAGA TGAACTCTAT GAAGCAGCCA TAATAGAAAC TAACTGCATT 
TACCGTCTCA TGGATGACAA ACTTGTTCCT GATGACGACT ACTGGGGGAA AATTCCGAAG 
TGCTCCCTTC TGCAACCCAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTTTACGTA 
TGGCATGGGA AAGAAGTCAC ATTAGCACAA CGAAAAATAG CATTTCAGCT GGCAAAGCAC 
TTATGGAATG GAACCTTTGA CTATGAGAAC TGTGACATCA ATCCCCTGGA TCCTGGAGAA 
TGCAATCCGC TTATCCCCAG AAAAGGACAG GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA 
CTTACTGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAGT TTCTGGATTG GACGGAACTG 
AAGAGATCGA ATGAGAAGAA CCCCGGGGAA CTTGCCCAGC ACAAGGAAGA CCCCAGGACT 
GATGTCAAGG CATACGATGT GACACGGATG GTGTCCATGC CCCAGACGAC AGCAGGCACC 
ATCCTGGACG GAGTGAACGT CGGCCGTGGC TATGGCCTGG TGGAAGGACA CGACAGGAGG 
CAGTTTGAGA TCACCAGCGT TTCCGTGGAT GTCTGGCACA TCCTGGAATT CGACTATAGC 
AGGCTCCCCA AACAAAGCAT CGGGCAGTTC CATGAGGGGG ATGCCTATGT GGTCAAGTGG 
AAGTTCATGG TGAGCACGGC AGTGGGAAGT CGCCAGAAGG GAGAGCACTC GGTGAGGGCA 
GCCGGCAAAG AGAAGTGCGT CTACTTCTTC TGGCAAGGCC GGCACTCCAC CGTGAGTGAG 
AAGGGCACGT CGGCGCTGAT GACGGTGGAG CTGGACGAGG AAAGGGGGGC CCAGGTCCAG 
GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC CTGCAGTGTT TCCAGGGGGG GATGGTGGTG 
CACTCGGGGA GGCGGGAAGA GGAAGAAGAA AATGTGCAAA GTGAGTGGCG GCTGTACTGC 
GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC 
CTGAGGTCCA GAACTTCCAT GGTGGTGCTT AACGTCAACA AGGCCCTCAT CTACCTGTGG 
CACGGATGCA AAGCCCAGGC CCACACGAAG GAGGTCGGAA GGACCGCTGC GAACAAGATC 
AAGGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTCAC AATACACGAG 
TGTGATGAAG GCTCCGAGCC ACTCGGATTC TGGGATGCCT TAGGAAGGAG AGACAGGAAA 
GCCTACGATT GCATGCTTCA AGATCCTGGA AGTTTTAACT TCGCGCCCCG CCTGTTCATC 
CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC ACAGAGTTTG TGTACCCTGC CCGAGCCCCC 
TCTGTGGTCA GTTCCATGCC CTTCCTGCAG GAAGATCTGT ACAGCGCGCC CCAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTG TACCTCTGGC AAGGCTGGTG GCCCATCGAG 
AACAAGATCA CTGGTTCCGC CCGCATCCGC TGGGCCTCCG ACCGGAAGAG TGCGATGGAG 
ACTGTGCTCC AGTACTGCAA AGGAAAAAAT CTCAAGAAAC CAGCCCCCAA GTCTTACCTT 
ATCCACGCTG GTCTGGAGCC CCTGACATTC ACCAATATGT TTCCCAGCTG GGAGCACAGA 
GAGGACATCG CTGAGATCAC AGAGATGGAC ACGGAAGTTT CCAATCAGAT CACCCTCGTG 
GAAGACGTCT TAGCCAAGCT CTGTAAAACC ATTTACCCGC TGGCCGACCT CCTGGCCAGG 
CCACTCCCGG AGGGGGTCGA TCCTCTGAAG CTTGAGATCT ATCTCACCGA CGAAGACTTC 
GAGTTTGCAC TAGACATGAC GAGGGATGAA TACAACGCCC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_017016580.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872069.1 Homo sapiens supervillin (SVIL), transcript variant X16, mRNA.

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CloneID	OHu91607
Clone ID Related Accession (Same CDS sequence)	XM_017016580.1
Accession Version	XM_017016580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGGTGCACT TCACATTCAG AAACGCCAAC TGTCGATGAT 
GAAGAAAAGG TGGATGAACG AGCCAAGCTG AGCGTCGCCG CCAAGAGGTT GCTTTTCAGG 
GAGATGGAAA AATCTTTTGA TGAACAAAAT GTTCCAAAGC GACGCTCAAG AAACACAGCT 
GTGGAGCAGA GGCTACGCCG TCTGCAGGAC AGGTCCCTCA CCCAGCCCAT CACCACTGAA 
GAGGTGGTCA TCGCAGCCAC TGAACCTATC CCCGCTTCGT GTTCTGGGGG CACCCACCCT 
GTAATGGCGA GACTTCCTAG CCCCACTGTA GCTAGGAGCG CTGTGCAGCC TGCCAGATTG 
CAGGCCTCTG CTCACCAAAA GGCCTTAGCC AAGGACCAGA CAAATGAGGG CAAAGAGCTT 
GCTGAGCAAG GAGAACCTGA TTCCTCCACT CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT 
AACAAATTGT CCCAGCCAGT CTCAAAAGCG ATTTCTACCC GGAACAGAAT AGACACGAGA 
CAGAGGAGAA TGAACGCTCG CTATCAAACT CAGCCAGTCA CACTGGGAGA GGTGGAGCAG 
GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA CCTGCCGTGA ACACATCAGT GTCTACCGTA 
GCATCCACGG TTGCTCCAAT GTATGCCGGA GATCTTCGCA CAAAGCCACC TCTTGACCAC 
AATGCAAGTG CCACTGACTA TAAGTTTTCT TCTTCAATAG AAAATTCGGA CTCTCCAGTT 
AGAAGCATTC TGAAATCGCA AGCTTGGCAG CCTTTGGTAG AGGGTAGCGA GAACAAGGGA 
ATGTTGAGAG AATATGGAGA GACAGAAAGC AAGAGAGCTT TGACAGGTCG AGACAGTGGG 
ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA GAAGCATCCT ACCCCATCCT GAACCGAGCC 
AGGGAAGGAG ACAGCCATAA GGAATCTAAA TATGCTGTTC CCAGAAGAGG AAGCCTGGAA 
CGGGCGAACC CTCCCATCAC CCACCTCGGG GATGAACCGA AGGAATTTTC CATGGCTAAA 
ATGAATGCAC AAGGAAACTT GGACTTGAGG GACAGGCTGC CCTTTGAAGA GAAGGTGGAG 
GTGGAGAATG TTATGAAAAG GAAGTTTTCA CTAAGAGCGG CAGAGTTCGG GGAGCCCACT 
TCCGAGCAGA CGGGGACAGC TGCTGGGAAA ACTATTGCTC AAACCACAGC CCCCGTGTCC 
TGGAAGCCCC AGGATTCTTC GGAACAGCCA CAGGAGAAGC TCTGCAAGAA TCCATGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAACGCCG ACAGGGGAGG GCCTTCTTGA CTCACCCAGC 
AAAACCATGT CTATTAAAGA AAGATTGGCA CTGTTGAAGA AAAGCGGGGA GGAAGATTGG 
AGAAACAGAC TCAGCAGGAG GCAGGAGGGC GGCAAGGCGC CGGCCAGCAG CCTGCACACC 
CAGGAAGCAG GGCGGTCCCT CATCAAGAAG GAAGAAGGAG GAGTTGCGGA TGATAGTGCC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAT GCTTCTACTT ATTCTCCTGC TATTCCTGCT 
GCCCATAAAT ACCTGTCTTT TGTGTCGATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872069.1
CDS	1241..7165
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_017016580.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016580.1

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGGTGCACT TCACATTCAG AAACGCCAAC TGTCGATGAT 
GAAGAAAAGG TGGATGAACG AGCCAAGCTG AGCGTCGCCG CCAAGAGGTT GCTTTTCAGG 
GAGATGGAAA AATCTTTTGA TGAACAAAAT GTTCCAAAGC GACGCTCAAG AAACACAGCT 
GTGGAGCAGA GGCTACGCCG TCTGCAGGAC AGGTCCCTCA CCCAGCCCAT CACCACTGAA 
GAGGTGGTCA TCGCAGCCAC TGAACCTATC CCCGCTTCGT GTTCTGGGGG CACCCACCCT 
GTAATGGCGA GACTTCCTAG CCCCACTGTA GCTAGGAGCG CTGTGCAGCC TGCCAGATTG 
CAGGCCTCTG CTCACCAAAA GGCCTTAGCC AAGGACCAGA CAAATGAGGG CAAAGAGCTT 
GCTGAGCAAG GAGAACCTGA TTCCTCCACT CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT 
AACAAATTGT CCCAGCCAGT CTCAAAAGCG ATTTCTACCC GGAACAGAAT AGACACGAGA 
CAGAGGAGAA TGAACGCTCG CTATCAAACT CAGCCAGTCA CACTGGGAGA GGTGGAGCAG 
GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA CCTGCCGTGA ACACATCAGT GTCTACCGTA 
GCATCCACGG TTGCTCCAAT GTATGCCGGA GATCTTCGCA CAAAGCCACC TCTTGACCAC 
AATGCAAGTG CCACTGACTA TAAGTTTTCT TCTTCAATAG AAAATTCGGA CTCTCCAGTT 
AGAAGCATTC TGAAATCGCA AGCTTGGCAG CCTTTGGTAG AGGGTAGCGA GAACAAGGGA 
ATGTTGAGAG AATATGGAGA GACAGAAAGC AAGAGAGCTT TGACAGGTCG AGACAGTGGG 
ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA GAAGCATCCT ACCCCATCCT GAACCGAGCC 
AGGGAAGGAG ACAGCCATAA GGAATCTAAA TATGCTGTTC CCAGAAGAGG AAGCCTGGAA 
CGGGCGAACC CTCCCATCAC CCACCTCGGG GATGAACCGA AGGAATTTTC CATGGCTAAA 
ATGAATGCAC AAGGAAACTT GGACTTGAGG GACAGGCTGC CCTTTGAAGA GAAGGTGGAG 
GTGGAGAATG TTATGAAAAG GAAGTTTTCA CTAAGAGCGG CAGAGTTCGG GGAGCCCACT 
TCCGAGCAGA CGGGGACAGC TGCTGGGAAA ACTATTGCTC AAACCACAGC CCCCGTGTCC 
TGGAAGCCCC AGGATTCTTC GGAACAGCCA CAGGAGAAGC TCTGCAAGAA TCCATGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAACGCCG ACAGGGGAGG GCCTTCTTGA CTCACCCAGC 
AAAACCATGT CTATTAAAGA AAGATTGGCA CTGTTGAAGA AAAGCGGGGA GGAAGATTGG 
AGAAACAGAC TCAGCAGGAG GCAGGAGGGC GGCAAGGCGC CGGCCAGCAG CCTGCACACC 
CAGGAAGCAG GGCGGTCCCT CATCAAGAAG GAAGAAGGAG GAGTTGCGGA TGATAGTGCC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAT GCTTCTACTT ATTCTCCTGC TATTCCTGCT 
GCCCATAAAT ACCTGTCTTT TGTGTCGATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519637.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517939.2 Homo sapiens supervillin (SVIL), transcript variant X17, mRNA.

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CloneID	OHu91608
Clone ID Related Accession (Same CDS sequence)	XM_011519637.2
Accession Version	XM_011519637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGGTGCACTT CACATTCAGA AACGCCAACT GTCGATGATG AAGAAAAGGT GGATGAACGA 
GCCAAGCTGA GCGTCGCCGC CAAGAGGTTG CTTTTCAGGG AGATGGAAAA ATCTTTTGAT 
GAACAAAATG TTCCAAAGCG ACGCTCAAGA AACACAGCTG TGGAGCAGAG GCTACGCCGT 
CTGCAGGACA GGTCCCTCAC CCAGCCCATC ACCACTGAAG AGGTGGTCAT CGCAGCCACT 
GAACCTATCC CCGCTTCGTG TTCTGGGGGC ACCCACCCTG TAATGGCGAG ACTTCCTAGC 
CCCACTGTAG CTAGGAGCGC TGTGCAGCCT GCCAGATTGC AGGCCTCTGC TCACCAAAAG 
GCCTTAGCCA AGGACCAGAC AAATGAGGGC AAAGAGCTTG CTGAGCAAGG AGAACCTGAT 
TCCTCCACTC TAAGCTTGGC CGAAAAGTTG GCCTTGTTTA ACAAATTGTC CCAGCCAGTC 
TCAAAAGCGA TTTCTACCCG GAACAGAATA GACACGAGAC AGAGGAGAAT GAACGCTCGC 
TATCAAACTC AGCCAGTCAC ACTGGGAGAG GTGGAGCAGG TGCAGAGTGG AAAGCTCATT 
CCTTTCTCAC CTGCCGTGAA CACATCAGTG TCTACCGTAG CATCCACGGT TGCTCCAATG 
TATGCCGGAG ATCTTCGCAC AAAGCCACCT CTTGACCACA ATGCAAGTGC CACTGACTAT 
AAGTTTTCTT CTTCAATAGA AAATTCGGAC TCTCCAGTTA GAAGCATTCT GAAATCGCAA 
GCTTGGCAGC CTTTGGTAGA GGGTAGCGAG AACAAGGGAA TGTTGAGAGA ATATGGAGAG 
ACAGAAAGCA AGAGAGCTTT GACAGGTCGA GACAGTGGGA TGGAGAAGTA TGGGTCCTTT 
GAGGAAGCAG AAGCATCCTA CCCCATCCTG AACCGAGCCA GGGAAGGAGA CAGCCATAAG 
GAATCTAAAT ATGCTGTTCC CAGAAGAGGA AGCCTGGAAC GGGCGAACCC TCCCATCACC 
CACCTCGGGG ATGAACCGAA GGAATTTTCC ATGGCTAAAA TGAATGCACA AGGAAACTTG 
GACTTGAGGG ACAGGCTGCC CTTTGAAGAG AAGGTGGAGG TGGAGAATGT TATGAAAAGG 
AAGTTTTCAC TAAGAGCGGC AGAGTTCGGG GAGCCCACTT CCGAGCAGAC GGGGACAGCT 
GCTGGGAAAA CTATTGCTCA AACCACAGCC CCCGTGTCCT GGAAGCCCCA GGATTCTTCG 
GAACAGCCAC AGGAGAAGCT CTGCAAGAAT CCATGTGCGA TGTTTGCTGC TGGAGAGATC 
AAAACGCCGA CAGGGGAGGG CCTTCTTGAC TCACCCAGCA AAACCATGTC TATTAAAGAA 
AGATTGGCAC TGTTGAAGAA AAGCGGGGAG GAAGATTGGA GAAACAGACT CAGCAGGAGG 
CAGGAGGGCG GCAAGGCGCC GGCCAGCAGC CTGCACACCC AGGAAGCAGG GCGGTCCCTC 
ATCAAGAAGG AAGAAGGAGG AGTTGCGGAT GATAGTGCCA TTTCTAATCT GCTTTGGGAA 
CCTGTATATG CTTCTACTTA TTCTCCTGCT ATTCCTGCTG CCCATAAATA CCTGTCTTTT 
GTGTCGATTA ATCAGCGGGT CACAGAAAGT CGAGAGAGCC AAATGACGAT TGAGGAGAGG 
AAGCAGCTCA TCACTGTGAG AGAGGAGGCC TGGAAGACGA GAGGCAGAGG AGCGGCCAAC 
GACTCGACCC AGTTCACTGT GGCTGGCAGG ATGGTGAAGA AAGGTTTGGC GTCACCTACT 
GCCATAACCC CAGTAGCCTC ACCCATTTGC GGTAAAACAA GAGGCACCAC ACCCGTTTCC 
AAACCCCTGG AAGATATCGA AGCCAGACCA GATATGCAGT TAGAATCGGA CCTGAAGTTG 
GACAGGCTGG AAACCTTTCT AAGAAGGCTG AATAACAAAG TTGGCGGGAT GCACGAAACG 
GTGCTCACTG TCACCGGCAA ATCTGTGAAG GAGGTGATGA AGCCAGATGA TGATGAAACC 
TTTGCCAAAT TTTACCGCAG CGTGGATTAT AATATGCCAA GAAGTCCTGT GGAGATGGAT 
GAGGACTTCG ATGTCATTTT CGATCCTTAT GCACCCAAAT TGACGTCTTC CGTGGCCGAG 
CACAAGCGGG CAGTTAGGCC CAAGCGCCGG GTTCAGGCCT CCAAAAACCC CCTGAAAATG 
CTGGCGGCAA GAGAAGATCT CCTTCAGGAA TACACTGAGC AGAGATTAAA CGTTGCCTTC 
ATGGAGTCAA AGCGGATGAA AGTAGAAAAG ATGTCTTCCA ACTCCAACTT CTCAGAAGTC 
ACCCTGGCGG GTTTAGCCAG TAAAGAAAAC TTCAGCAACG TCAGCCTGCG GAGCGTCAAC 
CTGACGGAAC AGAACTCTAA CAACAGCGCC GTGCCCTACA AGAGGCTGAT GCTGTTGCAG 
ATTAAAGGAA GAAGACATGT GCAGACCAGG CTGGTGGAAC CTCGAGCTTC GGCGCTCAAC 
AGTGGGGACT GCTTCCTCCT GCTCTCTCCC CACTGCTGCT TCCTGTGGGT AGGAGAGTTT 
GCAAACGTCA TAGAAAAGGC GAAGGCCTCA GAACTTGCAA CTTTAATTCA GACAAAGAGG 
GAACTTGGTT GTAGAGCTAC TTATATCCAA ACCATTGAAG AAGGAATTAA TACACACACT 
CATGCAGCCA AAGACTTCTG GAAGCTTCTG GGTGGCCAAA CCAGTTACCA ATCTGCTGGA 
GACCCAAAAG AAGATGAACT CTATGAAGCA GCCATAATAG AAACTAACTG CATTTACCGT 
CTCATGGATG ACAAACTTGT TCCTGATGAC GACTACTGGG GGAAAATTCC GAAGTGCTCC 
CTTCTGCAAC CCAAAGAGGT ACTGGTGTTT GATTTTGGTA GTGAAGTTTA CGTATGGCAT 
GGGAAAGAAG TCACATTAGC ACAACGAAAA ATAGCATTTC AGCTGGCAAA GCACTTATGG 
AATGGAACCT TTGACTATGA GAACTGTGAC ATCAATCCCC TGGATCCTGG AGAATGCAAT 
CCGCTTATCC CCAGAAAAGG ACAGGGGCGG CCCGACTGGG CGATATTTGG GAGACTTACT 
GAACACAATG AGACGATTTT GTTCAAAGAG AAGTTTCTGG ATTGGACGGA ACTGAAGAGA 
TCGAATGAGA AGAACCCCGG GGAACTTGCC CAGCACAAGG AAGACCCCAG GACTGATGTC 
AAGGCATACG ATGTGACACG GATGGTGTCC ATGCCCCAGA CGACAGCAGG CACCATCCTG 
GACGGAGTGA ACGTCGGCCG TGGCTATGGC CTGGTGGAAG GACACGACAG GAGGCAGTTT 
GAGATCACCA GCGTTTCCGT GGATGTCTGG CACATCCTGG AATTCGACTA TAGCAGGCTC 
CCCAAACAAA GCATCGGGCA GTTCCATGAG GGGGATGCCT ATGTGGTCAA GTGGAAGTTC 
ATGGTGAGCA CGGCAGTGGG AAGTCGCCAG AAGGGAGAGC ACTCGGTGAG GGCAGCCGGC 
AAAGAGAAGT GCGTCTACTT CTTCTGGCAA GGCCGGCACT CCACCGTGAG TGAGAAGGGC 
ACGTCGGCGC TGATGACGGT GGAGCTGGAC GAGGAAAGGG GGGCCCAGGT CCAGGTTCTC 
CAGGGAAAGG AGCCCCCCTG TTTCCTGCAG TGTTTCCAGG GGGGGATGGT GGTGCACTCG 
GGGAGGCGGG AAGAGGAAGA AGAAAATGTG CAAAGTGAGT GGCGGCTGTA CTGCGTGCGT 
GGAGAGGTGC CCGTGGAAGG GAATTTGCTG GAAGTGGCCT GTCACTGTAG CAGCCTGAGG 
TCCAGAACTT CCATGGTGGT GCTTAACGTC AACAAGGCCC TCATCTACCT GTGGCACGGA 
TGCAAAGCCC AGGCCCACAC GAAGGAGGTC GGAAGGACCG CTGCGAACAA GATCAAGGAA 
CAATGTCCCC TGGAAGCAGG ACTGCATAGT AGCAGCAAAG TCACAATACA CGAGTGTGAT 
GAAGGCTCCG AGCCACTCGG ATTCTGGGAT GCCTTAGGAA GGAGAGACAG GAAAGCCTAC 
GATTGCATGC TTCAAGATCC TGGAAGTTTT AACTTCGCGC CCCGCCTGTT CATCCTCAGC 
AGCTCCTCTG GGGATTTTGC AGCCACAGAG TTTGTGTACC CTGCCCGAGC CCCCTCTGTG 
GTCAGTTCCA TGCCCTTCCT GCAGGAAGAT CTGTACAGCG CGCCCCAGCC AGCACTTTTC 
CTTGTTGACA ATCACCACGA GGTGTACCTC TGGCAAGGCT GGTGGCCCAT CGAGAACAAG 
ATCACTGGTT CCGCCCGCAT CCGCTGGGCC TCCGACCGGA AGAGTGCGAT GGAGACTGTG 
CTCCAGTACT GCAAAGGAAA AAATCTCAAG AAACCAGCCC CCAAGTCTTA CCTTATCCAC 
GCTGGTCTGG AGCCCCTGAC ATTCACCAAT ATGTTTCCCA GCTGGGAGCA CAGAGAGGAC 
ATCGCTGAGA TCACAGAGAT GGACACGGAA GTTTCCAATC AGATCACCCT CGTGGAAGAC 
GTCTTAGCCA AGCTCTGTAA AACCATTTAC CCGCTGGCCG ACCTCCTGGC CAGGCCACTC 
CCGGAGGGGG TCGATCCTCT GAAGCTTGAG ATCTATCTCA CCGACGAAGA CTTCGAGTTT 
GCACTAGACA TGACGAGGGA TGAATACAAC GCCCTGCCCG CCTGGAAGCA GGTGAACCTG 
AAGAAAGCAA AAGGCCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517939.2
CDS	1241..7084
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519637.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519637.2

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGGTGCACTT CACATTCAGA AACGCCAACT GTCGATGATG AAGAAAAGGT GGATGAACGA 
GCCAAGCTGA GCGTCGCCGC CAAGAGGTTG CTTTTCAGGG AGATGGAAAA ATCTTTTGAT 
GAACAAAATG TTCCAAAGCG ACGCTCAAGA AACACAGCTG TGGAGCAGAG GCTACGCCGT 
CTGCAGGACA GGTCCCTCAC CCAGCCCATC ACCACTGAAG AGGTGGTCAT CGCAGCCACT 
GAACCTATCC CCGCTTCGTG TTCTGGGGGC ACCCACCCTG TAATGGCGAG ACTTCCTAGC 
CCCACTGTAG CTAGGAGCGC TGTGCAGCCT GCCAGATTGC AGGCCTCTGC TCACCAAAAG 
GCCTTAGCCA AGGACCAGAC AAATGAGGGC AAAGAGCTTG CTGAGCAAGG AGAACCTGAT 
TCCTCCACTC TAAGCTTGGC CGAAAAGTTG GCCTTGTTTA ACAAATTGTC CCAGCCAGTC 
TCAAAAGCGA TTTCTACCCG GAACAGAATA GACACGAGAC AGAGGAGAAT GAACGCTCGC 
TATCAAACTC AGCCAGTCAC ACTGGGAGAG GTGGAGCAGG TGCAGAGTGG AAAGCTCATT 
CCTTTCTCAC CTGCCGTGAA CACATCAGTG TCTACCGTAG CATCCACGGT TGCTCCAATG 
TATGCCGGAG ATCTTCGCAC AAAGCCACCT CTTGACCACA ATGCAAGTGC CACTGACTAT 
AAGTTTTCTT CTTCAATAGA AAATTCGGAC TCTCCAGTTA GAAGCATTCT GAAATCGCAA 
GCTTGGCAGC CTTTGGTAGA GGGTAGCGAG AACAAGGGAA TGTTGAGAGA ATATGGAGAG 
ACAGAAAGCA AGAGAGCTTT GACAGGTCGA GACAGTGGGA TGGAGAAGTA TGGGTCCTTT 
GAGGAAGCAG AAGCATCCTA CCCCATCCTG AACCGAGCCA GGGAAGGAGA CAGCCATAAG 
GAATCTAAAT ATGCTGTTCC CAGAAGAGGA AGCCTGGAAC GGGCGAACCC TCCCATCACC 
CACCTCGGGG ATGAACCGAA GGAATTTTCC ATGGCTAAAA TGAATGCACA AGGAAACTTG 
GACTTGAGGG ACAGGCTGCC CTTTGAAGAG AAGGTGGAGG TGGAGAATGT TATGAAAAGG 
AAGTTTTCAC TAAGAGCGGC AGAGTTCGGG GAGCCCACTT CCGAGCAGAC GGGGACAGCT 
GCTGGGAAAA CTATTGCTCA AACCACAGCC CCCGTGTCCT GGAAGCCCCA GGATTCTTCG 
GAACAGCCAC AGGAGAAGCT CTGCAAGAAT CCATGTGCGA TGTTTGCTGC TGGAGAGATC 
AAAACGCCGA CAGGGGAGGG CCTTCTTGAC TCACCCAGCA AAACCATGTC TATTAAAGAA 
AGATTGGCAC TGTTGAAGAA AAGCGGGGAG GAAGATTGGA GAAACAGACT CAGCAGGAGG 
CAGGAGGGCG GCAAGGCGCC GGCCAGCAGC CTGCACACCC AGGAAGCAGG GCGGTCCCTC 
ATCAAGAAGG AAGAAGGAGG AGTTGCGGAT GATAGTGCCA TTTCTAATCT GCTTTGGGAA 
CCTGTATATG CTTCTACTTA TTCTCCTGCT ATTCCTGCTG CCCATAAATA CCTGTCTTTT 
GTGTCGATTA ATCAGCGGGT CACAGAAAGT CGAGAGAGCC AAATGACGAT TGAGGAGAGG 
AAGCAGCTCA TCACTGTGAG AGAGGAGGCC TGGAAGACGA GAGGCAGAGG AGCGGCCAAC 
GACTCGACCC AGTTCACTGT GGCTGGCAGG ATGGTGAAGA AAGGTTTGGC GTCACCTACT 
GCCATAACCC CAGTAGCCTC ACCCATTTGC GGTAAAACAA GAGGCACCAC ACCCGTTTCC 
AAACCCCTGG AAGATATCGA AGCCAGACCA GATATGCAGT TAGAATCGGA CCTGAAGTTG 
GACAGGCTGG AAACCTTTCT AAGAAGGCTG AATAACAAAG TTGGCGGGAT GCACGAAACG 
GTGCTCACTG TCACCGGCAA ATCTGTGAAG GAGGTGATGA AGCCAGATGA TGATGAAACC 
TTTGCCAAAT TTTACCGCAG CGTGGATTAT AATATGCCAA GAAGTCCTGT GGAGATGGAT 
GAGGACTTCG ATGTCATTTT CGATCCTTAT GCACCCAAAT TGACGTCTTC CGTGGCCGAG 
CACAAGCGGG CAGTTAGGCC CAAGCGCCGG GTTCAGGCCT CCAAAAACCC CCTGAAAATG 
CTGGCGGCAA GAGAAGATCT CCTTCAGGAA TACACTGAGC AGAGATTAAA CGTTGCCTTC 
ATGGAGTCAA AGCGGATGAA AGTAGAAAAG ATGTCTTCCA ACTCCAACTT CTCAGAAGTC 
ACCCTGGCGG GTTTAGCCAG TAAAGAAAAC TTCAGCAACG TCAGCCTGCG GAGCGTCAAC 
CTGACGGAAC AGAACTCTAA CAACAGCGCC GTGCCCTACA AGAGGCTGAT GCTGTTGCAG 
ATTAAAGGAA GAAGACATGT GCAGACCAGG CTGGTGGAAC CTCGAGCTTC GGCGCTCAAC 
AGTGGGGACT GCTTCCTCCT GCTCTCTCCC CACTGCTGCT TCCTGTGGGT AGGAGAGTTT 
GCAAACGTCA TAGAAAAGGC GAAGGCCTCA GAACTTGCAA CTTTAATTCA GACAAAGAGG 
GAACTTGGTT GTAGAGCTAC TTATATCCAA ACCATTGAAG AAGGAATTAA TACACACACT 
CATGCAGCCA AAGACTTCTG GAAGCTTCTG GGTGGCCAAA CCAGTTACCA ATCTGCTGGA 
GACCCAAAAG AAGATGAACT CTATGAAGCA GCCATAATAG AAACTAACTG CATTTACCGT 
CTCATGGATG ACAAACTTGT TCCTGATGAC GACTACTGGG GGAAAATTCC GAAGTGCTCC 
CTTCTGCAAC CCAAAGAGGT ACTGGTGTTT GATTTTGGTA GTGAAGTTTA CGTATGGCAT 
GGGAAAGAAG TCACATTAGC ACAACGAAAA ATAGCATTTC AGCTGGCAAA GCACTTATGG 
AATGGAACCT TTGACTATGA GAACTGTGAC ATCAATCCCC TGGATCCTGG AGAATGCAAT 
CCGCTTATCC CCAGAAAAGG ACAGGGGCGG CCCGACTGGG CGATATTTGG GAGACTTACT 
GAACACAATG AGACGATTTT GTTCAAAGAG AAGTTTCTGG ATTGGACGGA ACTGAAGAGA 
TCGAATGAGA AGAACCCCGG GGAACTTGCC CAGCACAAGG AAGACCCCAG GACTGATGTC 
AAGGCATACG ATGTGACACG GATGGTGTCC ATGCCCCAGA CGACAGCAGG CACCATCCTG 
GACGGAGTGA ACGTCGGCCG TGGCTATGGC CTGGTGGAAG GACACGACAG GAGGCAGTTT 
GAGATCACCA GCGTTTCCGT GGATGTCTGG CACATCCTGG AATTCGACTA TAGCAGGCTC 
CCCAAACAAA GCATCGGGCA GTTCCATGAG GGGGATGCCT ATGTGGTCAA GTGGAAGTTC 
ATGGTGAGCA CGGCAGTGGG AAGTCGCCAG AAGGGAGAGC ACTCGGTGAG GGCAGCCGGC 
AAAGAGAAGT GCGTCTACTT CTTCTGGCAA GGCCGGCACT CCACCGTGAG TGAGAAGGGC 
ACGTCGGCGC TGATGACGGT GGAGCTGGAC GAGGAAAGGG GGGCCCAGGT CCAGGTTCTC 
CAGGGAAAGG AGCCCCCCTG TTTCCTGCAG TGTTTCCAGG GGGGGATGGT GGTGCACTCG 
GGGAGGCGGG AAGAGGAAGA AGAAAATGTG CAAAGTGAGT GGCGGCTGTA CTGCGTGCGT 
GGAGAGGTGC CCGTGGAAGG GAATTTGCTG GAAGTGGCCT GTCACTGTAG CAGCCTGAGG 
TCCAGAACTT CCATGGTGGT GCTTAACGTC AACAAGGCCC TCATCTACCT GTGGCACGGA 
TGCAAAGCCC AGGCCCACAC GAAGGAGGTC GGAAGGACCG CTGCGAACAA GATCAAGGAA 
CAATGTCCCC TGGAAGCAGG ACTGCATAGT AGCAGCAAAG TCACAATACA CGAGTGTGAT 
GAAGGCTCCG AGCCACTCGG ATTCTGGGAT GCCTTAGGAA GGAGAGACAG GAAAGCCTAC 
GATTGCATGC TTCAAGATCC TGGAAGTTTT AACTTCGCGC CCCGCCTGTT CATCCTCAGC 
AGCTCCTCTG GGGATTTTGC AGCCACAGAG TTTGTGTACC CTGCCCGAGC CCCCTCTGTG 
GTCAGTTCCA TGCCCTTCCT GCAGGAAGAT CTGTACAGCG CGCCCCAGCC AGCACTTTTC 
CTTGTTGACA ATCACCACGA GGTGTACCTC TGGCAAGGCT GGTGGCCCAT CGAGAACAAG 
ATCACTGGTT CCGCCCGCAT CCGCTGGGCC TCCGACCGGA AGAGTGCGAT GGAGACTGTG 
CTCCAGTACT GCAAAGGAAA AAATCTCAAG AAACCAGCCC CCAAGTCTTA CCTTATCCAC 
GCTGGTCTGG AGCCCCTGAC ATTCACCAAT ATGTTTCCCA GCTGGGAGCA CAGAGAGGAC 
ATCGCTGAGA TCACAGAGAT GGACACGGAA GTTTCCAATC AGATCACCCT CGTGGAAGAC 
GTCTTAGCCA AGCTCTGTAA AACCATTTAC CCGCTGGCCG ACCTCCTGGC CAGGCCACTC 
CCGGAGGGGG TCGATCCTCT GAAGCTTGAG ATCTATCTCA CCGACGAAGA CTTCGAGTTT 
GCACTAGACA TGACGAGGGA TGAATACAAC GCCCTGCCCG CCTGGAAGCA GGTGAACCTG 
AAGAAAGCAA AAGGCCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519638.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517940.2 Homo sapiens supervillin (SVIL), transcript variant X18, mRNA.

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CloneID	OHu91609
Clone ID Related Accession (Same CDS sequence)	XM_011519638.2
Accession Version	XM_011519638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGGTGCACTT CACATTCAGA AACGCCAACT GTCGATGATG AAGAAAAGGT GGATGAACGA 
GCCAAGCTGA GCGTCGCCGC CAAGAGGTTG CTTTTCAGGG AGATGGAAAA ATCTTTTGAT 
GAACAAAATG TTCCAAAGCG ACGCTCAAGA AACACAGCTG TGGAGCAGAG GCTACGCCGT 
CTGCAGGACA GGTCCCTCAC CCAGCCCATC ACCACTGAAG AGGTGGTCAT CGCAGCCACA 
TTGCAGGCCT CTGCTCACCA AAAGGCCTTA GCCAAGGACC AGACAAATGA GGGCAAAGAG 
CTTGCTGAGC AAGGAGAACC TGATTCCTCC ACTCTAAGCT TGGCCGAAAA GTTGGCCTTG 
TTTAACAAAT TGTCCCAGCC AGTCTCAAAA GCGATTTCTA CCCGGAACAG AATAGACACG 
AGACAGAGGA GAATGAACGC TCGCTATCAA ACTCAGCCAG TCACACTGGG AGAGGTGGAG 
CAGGTGCAGA GTGGAAAGCT CATTCCTTTC TCACCTGCCG TGAACACATC AGTGTCTACC 
GTAGCATCCA CGGTTGCTCC AATGTATGCC GGAGATCTTC GCACAAAGCC ACCTCTTGAC 
CACAATGCAA GTGCCACTGA CTATAAGTTT TCTTCTTCAA TAGAAAATTC GGACTCTCCA 
GTTAGAAGCA TTCTGAAATC GCAAGCTTGG CAGCCTTTGG TAGAGGGTAG CGAGAACAAG 
GGAATGTTGA GAGAATATGG AGAGACAGAA AGCAAGAGAG CTTTGACAGG TCGAGACAGT 
GGGATGGAGA AGTATGGGTC CTTTGAGGAA GCAGAAGCAT CCTACCCCAT CCTGAACCGA 
GCCAGGGAAG GAGACAGCCA TAAGGAATCT AAATATGCTG TTCCCAGAAG AGGAAGCCTG 
GAACGGGCGA ACCCTCCCAT CACCCACCTC GGGGATGAAC CGAAGGAATT TTCCATGGCT 
AAAATGAATG CACAAGGAAA CTTGGACTTG AGGGACAGGC TGCCCTTTGA AGAGAAGGTG 
GAGGTGGAGA ATGTTATGAA AAGGAAGTTT TCACTAAGAG CGGCAGAGTT CGGGGAGCCC 
ACTTCCGAGC AGACGGGGAC AGCTGCTGGG AAAACTATTG CTCAAACCAC AGCCCCCGTG 
TCCTGGAAGC CCCAGGATTC TTCGGAACAG CCACAGGAGA AGCTCTGCAA GAATCCATGT 
GCGATGTTTG CTGCTGGAGA GATCAAAACG CCGACAGGGG AGGGCCTTCT TGACTCACCC 
AGCAAAACCA TGTCTATTAA AGAAAGATTG GCACTGTTGA AGAAAAGCGG GGAGGAAGAT 
TGGAGAAACA GACTCAGCAG GAGGCAGGAG GGCGGCAAGG CGCCGGCCAG CAGCCTGCAC 
ACCCAGGAAG CAGGGCGGTC CCTCATCAAG AAGGAAGAAG GAGGAGTTGC GGATGATAGT 
GCCATTTCTA ATCTGCTTTG GGAACCTGTA TATGCTTCTA CTTATTCTCC TGCTATTCCT 
GCTGCCCATA AATACCTGTC TTTTGTGTCG ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAAATGA CGATTGAGGA GAGGAAGCAG CTCATCACTG TGAGAGAGGA GGCCTGGAAG 
ACGAGAGGCA GAGGAGCGGC CAACGACTCG ACCCAGTTCA CTGTGGCTGG CAGGATGGTG 
AAGAAAGGTT TGGCGTCACC TACTGCCATA ACCCCAGTAG CCTCACCCAT TTGCGGTAAA 
ACAAGAGGCA CCACACCCGT TTCCAAACCC CTGGAAGATA TCGAAGCCAG ACCAGATATG 
CAGTTAGAAT CGGACCTGAA GTTGGACAGG CTGGAAACCT TTCTAAGAAG GCTGAATAAC 
AAAGTTGGCG GGATGCACGA AACGGTGCTC ACTGTCACCG GCAAATCTGT GAAGGAGGTG 
ATGAAGCCAG ATGATGATGA AACCTTTGCC AAATTTTACC GCAGCGTGGA TTATAATATG 
CCAAGAAGTC CTGTGGAGAT GGATGAGGAC TTCGATGTCA TTTTCGATCC TTATGCACCC 
AAATTGACGT CTTCCGTGGC CGAGCACAAG CGGGCAGTTA GGCCCAAGCG CCGGGTTCAG 
GCCTCCAAAA ACCCCCTGAA AATGCTGGCG GCAAGAGAAG ATCTCCTTCA GGAATACACT 
GAGCAGAGAT TAAACGTTGC CTTCATGGAG TCAAAGCGGA TGAAAGTAGA AAAGATGTCT 
TCCAACTCCA ACTTCTCAGA AGTCACCCTG GCGGGTTTAG CCAGTAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAACT CTAACAACAG CGCCGTGCCC 
TACAAGAGGC TGATGCTGTT GCAGATTAAA GGAAGAAGAC ATGTGCAGAC CAGGCTGGTG 
GAACCTCGAG CTTCGGCGCT CAACAGTGGG GACTGCTTCC TCCTGCTCTC TCCCCACTGC 
TGCTTCCTGT GGGTAGGAGA GTTTGCAAAC GTCATAGAAA AGGCGAAGGC CTCAGAACTT 
GCAACTTTAA TTCAGACAAA GAGGGAACTT GGTTGTAGAG CTACTTATAT CCAAACCATT 
GAAGAAGGAA TTAATACACA CACTCATGCA GCCAAAGACT TCTGGAAGCT TCTGGGTGGC 
CAAACCAGTT ACCAATCTGC TGGAGACCCA AAAGAAGATG AACTCTATGA AGCAGCCATA 
ATAGAAACTA ACTGCATTTA CCGTCTCATG GATGACAAAC TTGTTCCTGA TGACGACTAC 
TGGGGGAAAA TTCCGAAGTG CTCCCTTCTG CAACCCAAAG AGGTACTGGT GTTTGATTTT 
GGTAGTGAAG TTTACGTATG GCATGGGAAA GAAGTCACAT TAGCACAACG AAAAATAGCA 
TTTCAGCTGG CAAAGCACTT ATGGAATGGA ACCTTTGACT ATGAGAACTG TGACATCAAT 
CCCCTGGATC CTGGAGAATG CAATCCGCTT ATCCCCAGAA AAGGACAGGG GCGGCCCGAC 
TGGGCGATAT TTGGGAGACT TACTGAACAC AATGAGACGA TTTTGTTCAA AGAGAAGTTT 
CTGGATTGGA CGGAACTGAA GAGATCGAAT GAGAAGAACC CCGGGGAACT TGCCCAGCAC 
AAGGAAGACC CCAGGACTGA TGTCAAGGCA TACGATGTGA CACGGATGGT GTCCATGCCC 
CAGACGACAG CAGGCACCAT CCTGGACGGA GTGAACGTCG GCCGTGGCTA TGGCCTGGTG 
GAAGGACACG ACAGGAGGCA GTTTGAGATC ACCAGCGTTT CCGTGGATGT CTGGCACATC 
CTGGAATTCG ACTATAGCAG GCTCCCCAAA CAAAGCATCG GGCAGTTCCA TGAGGGGGAT 
GCCTATGTGG TCAAGTGGAA GTTCATGGTG AGCACGGCAG TGGGAAGTCG CCAGAAGGGA 
GAGCACTCGG TGAGGGCAGC CGGCAAAGAG AAGTGCGTCT ACTTCTTCTG GCAAGGCCGG 
CACTCCACCG TGAGTGAGAA GGGCACGTCG GCGCTGATGA CGGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT TCTCCAGGGA AAGGAGCCCC CCTGTTTCCT GCAGTGTTTC 
CAGGGGGGGA TGGTGGTGCA CTCGGGGAGG CGGGAAGAGG AAGAAGAAAA TGTGCAAAGT 
GAGTGGCGGC TGTACTGCGT GCGTGGAGAG GTGCCCGTGG AAGGGAATTT GCTGGAAGTG 
GCCTGTCACT GTAGCAGCCT GAGGTCCAGA ACTTCCATGG TGGTGCTTAA CGTCAACAAG 
GCCCTCATCT ACCTGTGGCA CGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGAAGG 
ACCGCTGCGA ACAAGATCAA GGAACAATGT CCCCTGGAAG CAGGACTGCA TAGTAGCAGC 
AAAGTCACAA TACACGAGTG TGATGAAGGC TCCGAGCCAC TCGGATTCTG GGATGCCTTA 
GGAAGGAGAG ACAGGAAAGC CTACGATTGC ATGCTTCAAG ATCCTGGAAG TTTTAACTTC 
GCGCCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGGGATT TTGCAGCCAC AGAGTTTGTG 
TACCCTGCCC GAGCCCCCTC TGTGGTCAGT TCCATGCCCT TCCTGCAGGA AGATCTGTAC 
AGCGCGCCCC AGCCAGCACT TTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACT GGTTCCGCCC GCATCCGCTG GGCCTCCGAC 
CGGAAGAGTG CGATGGAGAC TGTGCTCCAG TACTGCAAAG GAAAAAATCT CAAGAAACCA 
GCCCCCAAGT CTTACCTTAT CCACGCTGGT CTGGAGCCCC TGACATTCAC CAATATGTTT 
CCCAGCTGGG AGCACAGAGA GGACATCGCT GAGATCACAG AGATGGACAC GGAAGTTTCC 
AATCAGATCA CCCTCGTGGA AGACGTCTTA GCCAAGCTCT GTAAAACCAT TTACCCGCTG 
GCCGACCTCC TGGCCAGGCC ACTCCCGGAG GGGGTCGATC CTCTGAAGCT TGAGATCTAT 
CTCACCGACG AAGACTTCGA GTTTGCACTA GACATGACGA GGGATGAATA CAACGCCCTG 
CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA GCAAAAGGCC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517940.2
CDS	1241..6988
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519638.2

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGGTGCACTT CACATTCAGA AACGCCAACT GTCGATGATG AAGAAAAGGT GGATGAACGA 
GCCAAGCTGA GCGTCGCCGC CAAGAGGTTG CTTTTCAGGG AGATGGAAAA ATCTTTTGAT 
GAACAAAATG TTCCAAAGCG ACGCTCAAGA AACACAGCTG TGGAGCAGAG GCTACGCCGT 
CTGCAGGACA GGTCCCTCAC CCAGCCCATC ACCACTGAAG AGGTGGTCAT CGCAGCCACA 
TTGCAGGCCT CTGCTCACCA AAAGGCCTTA GCCAAGGACC AGACAAATGA GGGCAAAGAG 
CTTGCTGAGC AAGGAGAACC TGATTCCTCC ACTCTAAGCT TGGCCGAAAA GTTGGCCTTG 
TTTAACAAAT TGTCCCAGCC AGTCTCAAAA GCGATTTCTA CCCGGAACAG AATAGACACG 
AGACAGAGGA GAATGAACGC TCGCTATCAA ACTCAGCCAG TCACACTGGG AGAGGTGGAG 
CAGGTGCAGA GTGGAAAGCT CATTCCTTTC TCACCTGCCG TGAACACATC AGTGTCTACC 
GTAGCATCCA CGGTTGCTCC AATGTATGCC GGAGATCTTC GCACAAAGCC ACCTCTTGAC 
CACAATGCAA GTGCCACTGA CTATAAGTTT TCTTCTTCAA TAGAAAATTC GGACTCTCCA 
GTTAGAAGCA TTCTGAAATC GCAAGCTTGG CAGCCTTTGG TAGAGGGTAG CGAGAACAAG 
GGAATGTTGA GAGAATATGG AGAGACAGAA AGCAAGAGAG CTTTGACAGG TCGAGACAGT 
GGGATGGAGA AGTATGGGTC CTTTGAGGAA GCAGAAGCAT CCTACCCCAT CCTGAACCGA 
GCCAGGGAAG GAGACAGCCA TAAGGAATCT AAATATGCTG TTCCCAGAAG AGGAAGCCTG 
GAACGGGCGA ACCCTCCCAT CACCCACCTC GGGGATGAAC CGAAGGAATT TTCCATGGCT 
AAAATGAATG CACAAGGAAA CTTGGACTTG AGGGACAGGC TGCCCTTTGA AGAGAAGGTG 
GAGGTGGAGA ATGTTATGAA AAGGAAGTTT TCACTAAGAG CGGCAGAGTT CGGGGAGCCC 
ACTTCCGAGC AGACGGGGAC AGCTGCTGGG AAAACTATTG CTCAAACCAC AGCCCCCGTG 
TCCTGGAAGC CCCAGGATTC TTCGGAACAG CCACAGGAGA AGCTCTGCAA GAATCCATGT 
GCGATGTTTG CTGCTGGAGA GATCAAAACG CCGACAGGGG AGGGCCTTCT TGACTCACCC 
AGCAAAACCA TGTCTATTAA AGAAAGATTG GCACTGTTGA AGAAAAGCGG GGAGGAAGAT 
TGGAGAAACA GACTCAGCAG GAGGCAGGAG GGCGGCAAGG CGCCGGCCAG CAGCCTGCAC 
ACCCAGGAAG CAGGGCGGTC CCTCATCAAG AAGGAAGAAG GAGGAGTTGC GGATGATAGT 
GCCATTTCTA ATCTGCTTTG GGAACCTGTA TATGCTTCTA CTTATTCTCC TGCTATTCCT 
GCTGCCCATA AATACCTGTC TTTTGTGTCG ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAAATGA CGATTGAGGA GAGGAAGCAG CTCATCACTG TGAGAGAGGA GGCCTGGAAG 
ACGAGAGGCA GAGGAGCGGC CAACGACTCG ACCCAGTTCA CTGTGGCTGG CAGGATGGTG 
AAGAAAGGTT TGGCGTCACC TACTGCCATA ACCCCAGTAG CCTCACCCAT TTGCGGTAAA 
ACAAGAGGCA CCACACCCGT TTCCAAACCC CTGGAAGATA TCGAAGCCAG ACCAGATATG 
CAGTTAGAAT CGGACCTGAA GTTGGACAGG CTGGAAACCT TTCTAAGAAG GCTGAATAAC 
AAAGTTGGCG GGATGCACGA AACGGTGCTC ACTGTCACCG GCAAATCTGT GAAGGAGGTG 
ATGAAGCCAG ATGATGATGA AACCTTTGCC AAATTTTACC GCAGCGTGGA TTATAATATG 
CCAAGAAGTC CTGTGGAGAT GGATGAGGAC TTCGATGTCA TTTTCGATCC TTATGCACCC 
AAATTGACGT CTTCCGTGGC CGAGCACAAG CGGGCAGTTA GGCCCAAGCG CCGGGTTCAG 
GCCTCCAAAA ACCCCCTGAA AATGCTGGCG GCAAGAGAAG ATCTCCTTCA GGAATACACT 
GAGCAGAGAT TAAACGTTGC CTTCATGGAG TCAAAGCGGA TGAAAGTAGA AAAGATGTCT 
TCCAACTCCA ACTTCTCAGA AGTCACCCTG GCGGGTTTAG CCAGTAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAACT CTAACAACAG CGCCGTGCCC 
TACAAGAGGC TGATGCTGTT GCAGATTAAA GGAAGAAGAC ATGTGCAGAC CAGGCTGGTG 
GAACCTCGAG CTTCGGCGCT CAACAGTGGG GACTGCTTCC TCCTGCTCTC TCCCCACTGC 
TGCTTCCTGT GGGTAGGAGA GTTTGCAAAC GTCATAGAAA AGGCGAAGGC CTCAGAACTT 
GCAACTTTAA TTCAGACAAA GAGGGAACTT GGTTGTAGAG CTACTTATAT CCAAACCATT 
GAAGAAGGAA TTAATACACA CACTCATGCA GCCAAAGACT TCTGGAAGCT TCTGGGTGGC 
CAAACCAGTT ACCAATCTGC TGGAGACCCA AAAGAAGATG AACTCTATGA AGCAGCCATA 
ATAGAAACTA ACTGCATTTA CCGTCTCATG GATGACAAAC TTGTTCCTGA TGACGACTAC 
TGGGGGAAAA TTCCGAAGTG CTCCCTTCTG CAACCCAAAG AGGTACTGGT GTTTGATTTT 
GGTAGTGAAG TTTACGTATG GCATGGGAAA GAAGTCACAT TAGCACAACG AAAAATAGCA 
TTTCAGCTGG CAAAGCACTT ATGGAATGGA ACCTTTGACT ATGAGAACTG TGACATCAAT 
CCCCTGGATC CTGGAGAATG CAATCCGCTT ATCCCCAGAA AAGGACAGGG GCGGCCCGAC 
TGGGCGATAT TTGGGAGACT TACTGAACAC AATGAGACGA TTTTGTTCAA AGAGAAGTTT 
CTGGATTGGA CGGAACTGAA GAGATCGAAT GAGAAGAACC CCGGGGAACT TGCCCAGCAC 
AAGGAAGACC CCAGGACTGA TGTCAAGGCA TACGATGTGA CACGGATGGT GTCCATGCCC 
CAGACGACAG CAGGCACCAT CCTGGACGGA GTGAACGTCG GCCGTGGCTA TGGCCTGGTG 
GAAGGACACG ACAGGAGGCA GTTTGAGATC ACCAGCGTTT CCGTGGATGT CTGGCACATC 
CTGGAATTCG ACTATAGCAG GCTCCCCAAA CAAAGCATCG GGCAGTTCCA TGAGGGGGAT 
GCCTATGTGG TCAAGTGGAA GTTCATGGTG AGCACGGCAG TGGGAAGTCG CCAGAAGGGA 
GAGCACTCGG TGAGGGCAGC CGGCAAAGAG AAGTGCGTCT ACTTCTTCTG GCAAGGCCGG 
CACTCCACCG TGAGTGAGAA GGGCACGTCG GCGCTGATGA CGGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT TCTCCAGGGA AAGGAGCCCC CCTGTTTCCT GCAGTGTTTC 
CAGGGGGGGA TGGTGGTGCA CTCGGGGAGG CGGGAAGAGG AAGAAGAAAA TGTGCAAAGT 
GAGTGGCGGC TGTACTGCGT GCGTGGAGAG GTGCCCGTGG AAGGGAATTT GCTGGAAGTG 
GCCTGTCACT GTAGCAGCCT GAGGTCCAGA ACTTCCATGG TGGTGCTTAA CGTCAACAAG 
GCCCTCATCT ACCTGTGGCA CGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGAAGG 
ACCGCTGCGA ACAAGATCAA GGAACAATGT CCCCTGGAAG CAGGACTGCA TAGTAGCAGC 
AAAGTCACAA TACACGAGTG TGATGAAGGC TCCGAGCCAC TCGGATTCTG GGATGCCTTA 
GGAAGGAGAG ACAGGAAAGC CTACGATTGC ATGCTTCAAG ATCCTGGAAG TTTTAACTTC 
GCGCCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGGGATT TTGCAGCCAC AGAGTTTGTG 
TACCCTGCCC GAGCCCCCTC TGTGGTCAGT TCCATGCCCT TCCTGCAGGA AGATCTGTAC 
AGCGCGCCCC AGCCAGCACT TTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACT GGTTCCGCCC GCATCCGCTG GGCCTCCGAC 
CGGAAGAGTG CGATGGAGAC TGTGCTCCAG TACTGCAAAG GAAAAAATCT CAAGAAACCA 
GCCCCCAAGT CTTACCTTAT CCACGCTGGT CTGGAGCCCC TGACATTCAC CAATATGTTT 
CCCAGCTGGG AGCACAGAGA GGACATCGCT GAGATCACAG AGATGGACAC GGAAGTTTCC 
AATCAGATCA CCCTCGTGGA AGACGTCTTA GCCAAGCTCT GTAAAACCAT TTACCCGCTG 
GCCGACCTCC TGGCCAGGCC ACTCCCGGAG GGGGTCGATC CTCTGAAGCT TGAGATCTAT 
CTCACCGACG AAGACTTCGA GTTTGCACTA GACATGACGA GGGATGAATA CAACGCCCTG 
CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA GCAAAAGGCC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_017016581.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872070.1 Homo sapiens supervillin (SVIL), transcript variant X19, mRNA.

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CloneID	OHu91610
Clone ID Related Accession (Same CDS sequence)	XM_017016581.1
Accession Version	XM_017016581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGGTGCACTT CACATTCAGA AACGCCAACT GTCGATGATG AAGAAAAGGT GGATGAACGA 
GCCAAGCTGA GCGTCGCCGC CAAGAGGTTG CTTTTCAGGG AGATGGAAAA ATCTTTTGAT 
GAACAAAATG TTCCAAAGCG ACGCTCAAGA AACACAGCTG TGGAGCAGAG GCTACGCCGT 
CTGCAGGACA GGTCCCTCAC CCAGCCCATC ACCACTGAAG AGGTGGTCAT CGCAGCCACT 
GAACCTATCC CCGCTTCGTG TTCTGGGGGC ACCCACCCTG TAATGGCGAG ACTTCCTAGC 
CCCACTGTAG CTAGGAGCGC TGTGCAGCCT GCCAGATTGC AGGCCTCTGC TCACCAAAAG 
GCCTTAGCCA AGGACCAGAC AAATGAGGGC AAAGAGCTTG CTGAGCAAGG AGAACCTGAT 
TCCTCCACTC TAAGCTTGGC CGAAAAGTTG GCCTTGTTTA ACAAATTGTC CCAGCCAGTC 
TCAAAAGCGA TTTCTACCCG GAACAGAATA GACACGAGAC AGAGGAGAAT GAACGCTCGC 
TATCAAACTC AGCCAGTCAC ACTGGGAGAG GTGGAGCAGG TGCAGAGTGG AAAGCTCATT 
CCTTTCTCAC CTGCCGTGAA CACATCAGTG TCTACCGTAG CATCCACGGT TGCTCCAATG 
TATGCCGGAG ATCTTCGCAC AAAGCCACCT CTTGACCACA ATGCAAGTGC CACTGACTAT 
AAGTTTTCTT CTTCAATAGA AAATTCGGAC TCTCCAGTTA GAAGCATTCT GAAATCGCAA 
GCTTGGCAGC CTTTGGTAGA GGGTAGCGAG AACAAGGGAA TGTTGAGAGA ATATGGAGAG 
ACAGAAAGCA AGAGAGCTTT GACAGGTCGA GACAGTGGGA TGGAGAAGTA TGGGTCCTTT 
GAGGAAGCAG AAGCATCCTA CCCCATCCTG AACCGAGCCA GGGAAGGAGA CAGCCATAAG 
GAATCTAAAT ATGCTGTTCC CAGAAGAGGA AGCCTGGAAC GGGCGAACCC TCCCATCACC 
CACCTCGGGG ATGAACCGAA GGAATTTTCC ATGGCTAAAA TGAATGCACA AGGAAACTTG 
GACTTGAGGG ACAGGCTGCC CTTTGAAGAG AAGGTGGAGG TGGAGAATGT TATGAAAAGG 
AAGTTTTCAC TAAGAGCGGC AGAGTTCGGG GAGCCCACTT CCGAGCAGAC GGGGACAGCT 
GCTGGGAAAA CTATTGCTCA AACCACAGCC CCCGTGTCCT GGAAGCCCCA GGATTCTTCG 
GAACAGCCAC AGGAGAAGCT CTGCAAGAAT CCATGTGCGA TGTTTGCTGC TGGAGAGATC 
AAAACGCCGA CAGGGGAGGG CCTTCTTGAC TCACCCAGCA AAACCATGTC TATTAAAGAA 
AGATTGGCAC TGTTGAAGAA AAGCGGGGAG GAAGATTGGA GAAACAGACT CAGCAGGAGG 
CAGGAGGGCG GCAAGGCGCC GGCCAGCAGC CTGCACACCC AGGAAGCAGG GCGGTCCCTC 
ATCAAGAAGC GGGTCACAGA AAGTCGAGAG AGCCAAATGA CGATTGAGGA GAGGAAGCAG 
CTCATCACTG TGAGAGAGGA GGCCTGGAAG ACGAGAGGCA GAGGAGCGGC CAACGACTCG 
ACCCAGTTCA CTGTGGCTGG CAGGATGGTG AAGAAAGGTT TGGCGTCACC TACTGCCATA 
ACCCCAGTAG CCTCACCCAT TTGCGGTAAA ACAAGAGGCA CCACACCCGT TTCCAAACCC 
CTGGAAGATA TCGAAGCCAG ACCAGATATG CAGTTAGAAT CGGACCTGAA GTTGGACAGG 
CTGGAAACCT TTCTAAGAAG GCTGAATAAC AAAGTTGGCG GGATGCACGA AACGGTGCTC 
ACTGTCACCG GCAAATCTGT GAAGGAGGTG ATGAAGCCAG ATGATGATGA AACCTTTGCC 
AAATTTTACC GCAGCGTGGA TTATAATATG CCAAGAAGTC CTGTGGAGAT GGATGAGGAC 
TTCGATGTCA TTTTCGATCC TTATGCACCC AAATTGACGT CTTCCGTGGC CGAGCACAAG 
CGGGCAGTTA GGCCCAAGCG CCGGGTTCAG GCCTCCAAAA ACCCCCTGAA AATGCTGGCG 
GCAAGAGAAG ATCTCCTTCA GGAATACACT GAGCAGAGAT TAAACGTTGC CTTCATGGAG 
TCAAAGCGGA TGAAAGTAGA AAAGATGTCT TCCAACTCCA ACTTCTCAGA AGTCACCCTG 
GCGGGTTTAG CCAGTAAAGA AAACTTCAGC AACGTCAGCC TGCGGAGCGT CAACCTGACG 
GAACAGAACT CTAACAACAG CGCCGTGCCC TACAAGAGGC TGATGCTGTT GCAGATTAAA 
GGAAGAAGAC ATGTGCAGAC CAGGCTGGTG GAACCTCGAG CTTCGGCGCT CAACAGTGGG 
GACTGCTTCC TCCTGCTCTC TCCCCACTGC TGCTTCCTGT GGGTAGGAGA GTTTGCAAAC 
GTCATAGAAA AGGCGAAGGC CTCAGAACTT GCAACTTTAA TTCAGACAAA GAGGGAACTT 
GGTTGTAGAG CTACTTATAT CCAAACCATT GAAGAAGGAA TTAATACACA CACTCATGCA 
GCCAAAGACT TCTGGAAGCT TCTGGGTGGC CAAACCAGTT ACCAATCTGC TGGAGACCCA 
AAAGAAGATG AACTCTATGA AGCAGCCATA ATAGAAACTA ACTGCATTTA CCGTCTCATG 
GATGACAAAC TTGTTCCTGA TGACGACTAC TGGGGGAAAA TTCCGAAGTG CTCCCTTCTG 
CAACCCAAAG AGGTACTGGT GTTTGATTTT GGTAGTGAAG TTTACGTATG GCATGGGAAA 
GAAGTCACAT TAGCACAACG AAAAATAGCA TTTCAGCTGG CAAAGCACTT ATGGAATGGA 
ACCTTTGACT ATGAGAACTG TGACATCAAT CCCCTGGATC CTGGAGAATG CAATCCGCTT 
ATCCCCAGAA AAGGACAGGG GCGGCCCGAC TGGGCGATAT TTGGGAGACT TACTGAACAC 
AATGAGACGA TTTTGTTCAA AGAGAAGTTT CTGGATTGGA CGGAACTGAA GAGATCGAAT 
GAGAAGAACC CCGGGGAACT TGCCCAGCAC AAGGAAGACC CCAGGACTGA TGTCAAGGCA 
TACGATGTGA CACGGATGGT GTCCATGCCC CAGACGACAG CAGGCACCAT CCTGGACGGA 
GTGAACGTCG GCCGTGGCTA TGGCCTGGTG GAAGGACACG ACAGGAGGCA GTTTGAGATC 
ACCAGCGTTT CCGTGGATGT CTGGCACATC CTGGAATTCG ACTATAGCAG GCTCCCCAAA 
CAAAGCATCG GGCAGTTCCA TGAGGGGGAT GCCTATGTGG TCAAGTGGAA GTTCATGGTG 
AGCACGGCAG TGGGAAGTCG CCAGAAGGGA GAGCACTCGG TGAGGGCAGC CGGCAAAGAG 
AAGTGCGTCT ACTTCTTCTG GCAAGGCCGG CACTCCACCG TGAGTGAGAA GGGCACGTCG 
GCGCTGATGA CGGTGGAGCT GGACGAGGAA AGGGGGGCCC AGGTCCAGGT TCTCCAGGGA 
AAGGAGCCCC CCTGTTTCCT GCAGTGTTTC CAGGGGGGGA TGGTGGTGCA CTCGGGGAGG 
CGGGAAGAGG AAGAAGAAAA TGTGCAAAGT GAGTGGCGGC TGTACTGCGT GCGTGGAGAG 
GTGCCCGTGG AAGGGAATTT GCTGGAAGTG GCCTGTCACT GTAGCAGCCT GAGGTCCAGA 
ACTTCCATGG TGGTGCTTAA CGTCAACAAG GCCCTCATCT ACCTGTGGCA CGGATGCAAA 
GCCCAGGCCC ACACGAAGGA GGTCGGAAGG ACCGCTGCGA ACAAGATCAA GGAACAATGT 
CCCCTGGAAG CAGGACTGCA TAGTAGCAGC AAAGTCACAA TACACGAGTG TGATGAAGGC 
TCCGAGCCAC TCGGATTCTG GGATGCCTTA GGAAGGAGAG ACAGGAAAGC CTACGATTGC 
ATGCTTCAAG ATCCTGGAAG TTTTAACTTC GCGCCCCGCC TGTTCATCCT CAGCAGCTCC 
TCTGGGGATT TTGCAGCCAC AGAGTTTGTG TACCCTGCCC GAGCCCCCTC TGTGGTCAGT 
TCCATGCCCT TCCTGCAGGA AGATCTGTAC AGCGCGCCCC AGCCAGCACT TTTCCTTGTT 
GACAATCACC ACGAGGTGTA CCTCTGGCAA GGCTGGTGGC CCATCGAGAA CAAGATCACT 
GGTTCCGCCC GCATCCGCTG GGCCTCCGAC CGGAAGAGTG CGATGGAGAC TGTGCTCCAG 
TACTGCAAAG GAAAAAATCT CAAGAAACCA GCCCCCAAGT CTTACCTTAT CCACGCTGGT 
CTGGAGCCCC TGACATTCAC CAATATGTTT CCCAGCTGGG AGCACAGAGA GGACATCGCT 
GAGATCACAG AGATGGACAC GGAAGTTTCC AATCAGATCA CCCTCGTGGA AGACGTCTTA 
GCCAAGCTCT GTAAAACCAT TTACCCGCTG GCCGACCTCC TGGCCAGGCC ACTCCCGGAG 
GGGGTCGATC CTCTGAAGCT TGAGATCTAT CTCACCGACG AAGACTTCGA GTTTGCACTA 
GACATGACGA GGGATGAATA CAACGCCCTG CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA 
GCAAAAGGCC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872070.1
CDS	1241..6958
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_017016581.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016581.1

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGGTGCACTT CACATTCAGA AACGCCAACT GTCGATGATG AAGAAAAGGT GGATGAACGA 
GCCAAGCTGA GCGTCGCCGC CAAGAGGTTG CTTTTCAGGG AGATGGAAAA ATCTTTTGAT 
GAACAAAATG TTCCAAAGCG ACGCTCAAGA AACACAGCTG TGGAGCAGAG GCTACGCCGT 
CTGCAGGACA GGTCCCTCAC CCAGCCCATC ACCACTGAAG AGGTGGTCAT CGCAGCCACT 
GAACCTATCC CCGCTTCGTG TTCTGGGGGC ACCCACCCTG TAATGGCGAG ACTTCCTAGC 
CCCACTGTAG CTAGGAGCGC TGTGCAGCCT GCCAGATTGC AGGCCTCTGC TCACCAAAAG 
GCCTTAGCCA AGGACCAGAC AAATGAGGGC AAAGAGCTTG CTGAGCAAGG AGAACCTGAT 
TCCTCCACTC TAAGCTTGGC CGAAAAGTTG GCCTTGTTTA ACAAATTGTC CCAGCCAGTC 
TCAAAAGCGA TTTCTACCCG GAACAGAATA GACACGAGAC AGAGGAGAAT GAACGCTCGC 
TATCAAACTC AGCCAGTCAC ACTGGGAGAG GTGGAGCAGG TGCAGAGTGG AAAGCTCATT 
CCTTTCTCAC CTGCCGTGAA CACATCAGTG TCTACCGTAG CATCCACGGT TGCTCCAATG 
TATGCCGGAG ATCTTCGCAC AAAGCCACCT CTTGACCACA ATGCAAGTGC CACTGACTAT 
AAGTTTTCTT CTTCAATAGA AAATTCGGAC TCTCCAGTTA GAAGCATTCT GAAATCGCAA 
GCTTGGCAGC CTTTGGTAGA GGGTAGCGAG AACAAGGGAA TGTTGAGAGA ATATGGAGAG 
ACAGAAAGCA AGAGAGCTTT GACAGGTCGA GACAGTGGGA TGGAGAAGTA TGGGTCCTTT 
GAGGAAGCAG AAGCATCCTA CCCCATCCTG AACCGAGCCA GGGAAGGAGA CAGCCATAAG 
GAATCTAAAT ATGCTGTTCC CAGAAGAGGA AGCCTGGAAC GGGCGAACCC TCCCATCACC 
CACCTCGGGG ATGAACCGAA GGAATTTTCC ATGGCTAAAA TGAATGCACA AGGAAACTTG 
GACTTGAGGG ACAGGCTGCC CTTTGAAGAG AAGGTGGAGG TGGAGAATGT TATGAAAAGG 
AAGTTTTCAC TAAGAGCGGC AGAGTTCGGG GAGCCCACTT CCGAGCAGAC GGGGACAGCT 
GCTGGGAAAA CTATTGCTCA AACCACAGCC CCCGTGTCCT GGAAGCCCCA GGATTCTTCG 
GAACAGCCAC AGGAGAAGCT CTGCAAGAAT CCATGTGCGA TGTTTGCTGC TGGAGAGATC 
AAAACGCCGA CAGGGGAGGG CCTTCTTGAC TCACCCAGCA AAACCATGTC TATTAAAGAA 
AGATTGGCAC TGTTGAAGAA AAGCGGGGAG GAAGATTGGA GAAACAGACT CAGCAGGAGG 
CAGGAGGGCG GCAAGGCGCC GGCCAGCAGC CTGCACACCC AGGAAGCAGG GCGGTCCCTC 
ATCAAGAAGC GGGTCACAGA AAGTCGAGAG AGCCAAATGA CGATTGAGGA GAGGAAGCAG 
CTCATCACTG TGAGAGAGGA GGCCTGGAAG ACGAGAGGCA GAGGAGCGGC CAACGACTCG 
ACCCAGTTCA CTGTGGCTGG CAGGATGGTG AAGAAAGGTT TGGCGTCACC TACTGCCATA 
ACCCCAGTAG CCTCACCCAT TTGCGGTAAA ACAAGAGGCA CCACACCCGT TTCCAAACCC 
CTGGAAGATA TCGAAGCCAG ACCAGATATG CAGTTAGAAT CGGACCTGAA GTTGGACAGG 
CTGGAAACCT TTCTAAGAAG GCTGAATAAC AAAGTTGGCG GGATGCACGA AACGGTGCTC 
ACTGTCACCG GCAAATCTGT GAAGGAGGTG ATGAAGCCAG ATGATGATGA AACCTTTGCC 
AAATTTTACC GCAGCGTGGA TTATAATATG CCAAGAAGTC CTGTGGAGAT GGATGAGGAC 
TTCGATGTCA TTTTCGATCC TTATGCACCC AAATTGACGT CTTCCGTGGC CGAGCACAAG 
CGGGCAGTTA GGCCCAAGCG CCGGGTTCAG GCCTCCAAAA ACCCCCTGAA AATGCTGGCG 
GCAAGAGAAG ATCTCCTTCA GGAATACACT GAGCAGAGAT TAAACGTTGC CTTCATGGAG 
TCAAAGCGGA TGAAAGTAGA AAAGATGTCT TCCAACTCCA ACTTCTCAGA AGTCACCCTG 
GCGGGTTTAG CCAGTAAAGA AAACTTCAGC AACGTCAGCC TGCGGAGCGT CAACCTGACG 
GAACAGAACT CTAACAACAG CGCCGTGCCC TACAAGAGGC TGATGCTGTT GCAGATTAAA 
GGAAGAAGAC ATGTGCAGAC CAGGCTGGTG GAACCTCGAG CTTCGGCGCT CAACAGTGGG 
GACTGCTTCC TCCTGCTCTC TCCCCACTGC TGCTTCCTGT GGGTAGGAGA GTTTGCAAAC 
GTCATAGAAA AGGCGAAGGC CTCAGAACTT GCAACTTTAA TTCAGACAAA GAGGGAACTT 
GGTTGTAGAG CTACTTATAT CCAAACCATT GAAGAAGGAA TTAATACACA CACTCATGCA 
GCCAAAGACT TCTGGAAGCT TCTGGGTGGC CAAACCAGTT ACCAATCTGC TGGAGACCCA 
AAAGAAGATG AACTCTATGA AGCAGCCATA ATAGAAACTA ACTGCATTTA CCGTCTCATG 
GATGACAAAC TTGTTCCTGA TGACGACTAC TGGGGGAAAA TTCCGAAGTG CTCCCTTCTG 
CAACCCAAAG AGGTACTGGT GTTTGATTTT GGTAGTGAAG TTTACGTATG GCATGGGAAA 
GAAGTCACAT TAGCACAACG AAAAATAGCA TTTCAGCTGG CAAAGCACTT ATGGAATGGA 
ACCTTTGACT ATGAGAACTG TGACATCAAT CCCCTGGATC CTGGAGAATG CAATCCGCTT 
ATCCCCAGAA AAGGACAGGG GCGGCCCGAC TGGGCGATAT TTGGGAGACT TACTGAACAC 
AATGAGACGA TTTTGTTCAA AGAGAAGTTT CTGGATTGGA CGGAACTGAA GAGATCGAAT 
GAGAAGAACC CCGGGGAACT TGCCCAGCAC AAGGAAGACC CCAGGACTGA TGTCAAGGCA 
TACGATGTGA CACGGATGGT GTCCATGCCC CAGACGACAG CAGGCACCAT CCTGGACGGA 
GTGAACGTCG GCCGTGGCTA TGGCCTGGTG GAAGGACACG ACAGGAGGCA GTTTGAGATC 
ACCAGCGTTT CCGTGGATGT CTGGCACATC CTGGAATTCG ACTATAGCAG GCTCCCCAAA 
CAAAGCATCG GGCAGTTCCA TGAGGGGGAT GCCTATGTGG TCAAGTGGAA GTTCATGGTG 
AGCACGGCAG TGGGAAGTCG CCAGAAGGGA GAGCACTCGG TGAGGGCAGC CGGCAAAGAG 
AAGTGCGTCT ACTTCTTCTG GCAAGGCCGG CACTCCACCG TGAGTGAGAA GGGCACGTCG 
GCGCTGATGA CGGTGGAGCT GGACGAGGAA AGGGGGGCCC AGGTCCAGGT TCTCCAGGGA 
AAGGAGCCCC CCTGTTTCCT GCAGTGTTTC CAGGGGGGGA TGGTGGTGCA CTCGGGGAGG 
CGGGAAGAGG AAGAAGAAAA TGTGCAAAGT GAGTGGCGGC TGTACTGCGT GCGTGGAGAG 
GTGCCCGTGG AAGGGAATTT GCTGGAAGTG GCCTGTCACT GTAGCAGCCT GAGGTCCAGA 
ACTTCCATGG TGGTGCTTAA CGTCAACAAG GCCCTCATCT ACCTGTGGCA CGGATGCAAA 
GCCCAGGCCC ACACGAAGGA GGTCGGAAGG ACCGCTGCGA ACAAGATCAA GGAACAATGT 
CCCCTGGAAG CAGGACTGCA TAGTAGCAGC AAAGTCACAA TACACGAGTG TGATGAAGGC 
TCCGAGCCAC TCGGATTCTG GGATGCCTTA GGAAGGAGAG ACAGGAAAGC CTACGATTGC 
ATGCTTCAAG ATCCTGGAAG TTTTAACTTC GCGCCCCGCC TGTTCATCCT CAGCAGCTCC 
TCTGGGGATT TTGCAGCCAC AGAGTTTGTG TACCCTGCCC GAGCCCCCTC TGTGGTCAGT 
TCCATGCCCT TCCTGCAGGA AGATCTGTAC AGCGCGCCCC AGCCAGCACT TTTCCTTGTT 
GACAATCACC ACGAGGTGTA CCTCTGGCAA GGCTGGTGGC CCATCGAGAA CAAGATCACT 
GGTTCCGCCC GCATCCGCTG GGCCTCCGAC CGGAAGAGTG CGATGGAGAC TGTGCTCCAG 
TACTGCAAAG GAAAAAATCT CAAGAAACCA GCCCCCAAGT CTTACCTTAT CCACGCTGGT 
CTGGAGCCCC TGACATTCAC CAATATGTTT CCCAGCTGGG AGCACAGAGA GGACATCGCT 
GAGATCACAG AGATGGACAC GGAAGTTTCC AATCAGATCA CCCTCGTGGA AGACGTCTTA 
GCCAAGCTCT GTAAAACCAT TTACCCGCTG GCCGACCTCC TGGCCAGGCC ACTCCCGGAG 
GGGGTCGATC CTCTGAAGCT TGAGATCTAT CTCACCGACG AAGACTTCGA GTTTGCACTA 
GACATGACGA GGGATGAATA CAACGCCCTG CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA 
GCAAAAGGCC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_017016582.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872071.1 Homo sapiens supervillin (SVIL), transcript variant X20, mRNA.

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CloneID	OHu91611
Clone ID Related Accession (Same CDS sequence)	XM_017016582.1
Accession Version	XM_017016582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGGTGCACTT CACATTCAGA AACGCCAACT GTCGATGATG AAGAAAAGGT GGATGAACGA 
GCCAAGCTGA GCGTCGCCGC CAAGAGGTTG CTTTTCAGGG AGATGGAAAA ATCTTTTGAT 
GAACAAAATG TTCCAAAGCG ACGCTCAAGA AACACAGCTG TGGAGCAGAG GCTACGCCGT 
CTGCAGGACA GGTCCCTCAC CCAGCCCATC ACCACTGAAG AGGTGGTCAT CGCAGCCACA 
TTGCAGGCCT CTGCTCACCA AAAGGCCTTA GCCAAGGACC AGACAAATGA GGGCAAAGAG 
CTTGCTGAGC AAGGAGAACC TGATTCCTCC ACTCTAAGCT TGGCCGAAAA GTTGGCCTTG 
TTTAACAAAT TGTCCCAGCC AGTCTCAAAA GCGATTTCTA CCCGGAACAG AATAGACACG 
AGACAGAGGA GAATGAACGC TCGCTATCAA ACTCAGCCAG TCACACTGGG AGAGGTGGAG 
CAGGTGCAGA GTGGAAAGCT CATTCCTTTC TCACCTGCCG TGAACACATC AGTGTCTACC 
GTAGCATCCA CGGTTGCTCC AATGTATGCC GGAGATCTTC GCACAAAGCC ACCTCTTGAC 
CACAATGCAA GTGCCACTGA CTATAAGTTT TCTTCTTCAA TAGAAAATTC GGACTCTCCA 
GTTAGAAGCA TTCTGAAATC GCAAGCTTGG CAGCCTTTGG TAGAGGGTAG CGAGAACAAG 
GGAATGTTGA GAGAATATGG AGAGACAGAA AGCAAGAGAG CTTTGACAGG TCGAGACAGT 
GGGATGGAGA AGTATGGGTC CTTTGAGGAA GCAGAAGCAT CCTACCCCAT CCTGAACCGA 
GCCAGGGAAG GAGACAGCCA TAAGGAATCT AAATATGCTG TTCCCAGAAG AGGAAGCCTG 
GAACGGGCGA ACCCTCCCAT CACCCACCTC GGGGATGAAC CGAAGGAATT TTCCATGGCT 
AAAATGAATG CACAAGGAAA CTTGGACTTG AGGGACAGGC TGCCCTTTGA AGAGAAGGTG 
GAGGTGGAGA ATGTTATGAA AAGGAAGTTT TCACTAAGAG CGGCAGAGTT CGGGGAGCCC 
ACTTCCGAGC AGACGGGGAC AGCTGCTGGG AAAACTATTG CTCAAACCAC AGCCCCCGTG 
TCCTGGAAGC CCCAGGATTC TTCGGAACAG CCACAGGAGA AGCTCTGCAA GAATCCATGT 
GCGATGTTTG CTGCTGGAGA GATCAAAACG CCGACAGGGG AGGGCCTTCT TGACTCACCC 
AGCAAAACCA TGTCTATTAA AGAAAGATTG GCACTGTTGA AGAAAAGCGG GGAGGAAGAT 
TGGAGAAACA GACTCAGCAG GAGGCAGGAG GGCGGCAAGG CGCCGGCCAG CAGCCTGCAC 
ACCCAGGAAG CAGGGCGGTC CCTCATCAAG AAGCGGGTCA CAGAAAGTCG AGAGAGCCAA 
ATGACGATTG AGGAGAGGAA GCAGCTCATC ACTGTGAGAG AGGAGGCCTG GAAGACGAGA 
GGCAGAGGAG CGGCCAACGA CTCGACCCAG TTCACTGTGG CTGGCAGGAT GGTGAAGAAA 
GGTTTGGCGT CACCTACTGC CATAACCCCA GTAGCCTCAC CCATTTGCGG TAAAACAAGA 
GGCACCACAC CCGTTTCCAA ACCCCTGGAA GATATCGAAG CCAGACCAGA TATGCAGTTA 
GAATCGGACC TGAAGTTGGA CAGGCTGGAA ACCTTTCTAA GAAGGCTGAA TAACAAAGTT 
GGCGGGATGC ACGAAACGGT GCTCACTGTC ACCGGCAAAT CTGTGAAGGA GGTGATGAAG 
CCAGATGATG ATGAAACCTT TGCCAAATTT TACCGCAGCG TGGATTATAA TATGCCAAGA 
AGTCCTGTGG AGATGGATGA GGACTTCGAT GTCATTTTCG ATCCTTATGC ACCCAAATTG 
ACGTCTTCCG TGGCCGAGCA CAAGCGGGCA GTTAGGCCCA AGCGCCGGGT TCAGGCCTCC 
AAAAACCCCC TGAAAATGCT GGCGGCAAGA GAAGATCTCC TTCAGGAATA CACTGAGCAG 
AGATTAAACG TTGCCTTCAT GGAGTCAAAG CGGATGAAAG TAGAAAAGAT GTCTTCCAAC 
TCCAACTTCT CAGAAGTCAC CCTGGCGGGT TTAGCCAGTA AAGAAAACTT CAGCAACGTC 
AGCCTGCGGA GCGTCAACCT GACGGAACAG AACTCTAACA ACAGCGCCGT GCCCTACAAG 
AGGCTGATGC TGTTGCAGAT TAAAGGAAGA AGACATGTGC AGACCAGGCT GGTGGAACCT 
CGAGCTTCGG CGCTCAACAG TGGGGACTGC TTCCTCCTGC TCTCTCCCCA CTGCTGCTTC 
CTGTGGGTAG GAGAGTTTGC AAACGTCATA GAAAAGGCGA AGGCCTCAGA ACTTGCAACT 
TTAATTCAGA CAAAGAGGGA ACTTGGTTGT AGAGCTACTT ATATCCAAAC CATTGAAGAA 
GGAATTAATA CACACACTCA TGCAGCCAAA GACTTCTGGA AGCTTCTGGG TGGCCAAACC 
AGTTACCAAT CTGCTGGAGA CCCAAAAGAA GATGAACTCT ATGAAGCAGC CATAATAGAA 
ACTAACTGCA TTTACCGTCT CATGGATGAC AAACTTGTTC CTGATGACGA CTACTGGGGG 
AAAATTCCGA AGTGCTCCCT TCTGCAACCC AAAGAGGTAC TGGTGTTTGA TTTTGGTAGT 
GAAGTTTACG TATGGCATGG GAAAGAAGTC ACATTAGCAC AACGAAAAAT AGCATTTCAG 
CTGGCAAAGC ACTTATGGAA TGGAACCTTT GACTATGAGA ACTGTGACAT CAATCCCCTG 
GATCCTGGAG AATGCAATCC GCTTATCCCC AGAAAAGGAC AGGGGCGGCC CGACTGGGCG 
ATATTTGGGA GACTTACTGA ACACAATGAG ACGATTTTGT TCAAAGAGAA GTTTCTGGAT 
TGGACGGAAC TGAAGAGATC GAATGAGAAG AACCCCGGGG AACTTGCCCA GCACAAGGAA 
GACCCCAGGA CTGATGTCAA GGCATACGAT GTGACACGGA TGGTGTCCAT GCCCCAGACG 
ACAGCAGGCA CCATCCTGGA CGGAGTGAAC GTCGGCCGTG GCTATGGCCT GGTGGAAGGA 
CACGACAGGA GGCAGTTTGA GATCACCAGC GTTTCCGTGG ATGTCTGGCA CATCCTGGAA 
TTCGACTATA GCAGGCTCCC CAAACAAAGC ATCGGGCAGT TCCATGAGGG GGATGCCTAT 
GTGGTCAAGT GGAAGTTCAT GGTGAGCACG GCAGTGGGAA GTCGCCAGAA GGGAGAGCAC 
TCGGTGAGGG CAGCCGGCAA AGAGAAGTGC GTCTACTTCT TCTGGCAAGG CCGGCACTCC 
ACCGTGAGTG AGAAGGGCAC GTCGGCGCTG ATGACGGTGG AGCTGGACGA GGAAAGGGGG 
GCCCAGGTCC AGGTTCTCCA GGGAAAGGAG CCCCCCTGTT TCCTGCAGTG TTTCCAGGGG 
GGGATGGTGG TGCACTCGGG GAGGCGGGAA GAGGAAGAAG AAAATGTGCA AAGTGAGTGG 
CGGCTGTACT GCGTGCGTGG AGAGGTGCCC GTGGAAGGGA ATTTGCTGGA AGTGGCCTGT 
CACTGTAGCA GCCTGAGGTC CAGAACTTCC ATGGTGGTGC TTAACGTCAA CAAGGCCCTC 
ATCTACCTGT GGCACGGATG CAAAGCCCAG GCCCACACGA AGGAGGTCGG AAGGACCGCT 
GCGAACAAGA TCAAGGAACA ATGTCCCCTG GAAGCAGGAC TGCATAGTAG CAGCAAAGTC 
ACAATACACG AGTGTGATGA AGGCTCCGAG CCACTCGGAT TCTGGGATGC CTTAGGAAGG 
AGAGACAGGA AAGCCTACGA TTGCATGCTT CAAGATCCTG GAAGTTTTAA CTTCGCGCCC 
CGCCTGTTCA TCCTCAGCAG CTCCTCTGGG GATTTTGCAG CCACAGAGTT TGTGTACCCT 
GCCCGAGCCC CCTCTGTGGT CAGTTCCATG CCCTTCCTGC AGGAAGATCT GTACAGCGCG 
CCCCAGCCAG CACTTTTCCT TGTTGACAAT CACCACGAGG TGTACCTCTG GCAAGGCTGG 
TGGCCCATCG AGAACAAGAT CACTGGTTCC GCCCGCATCC GCTGGGCCTC CGACCGGAAG 
AGTGCGATGG AGACTGTGCT CCAGTACTGC AAAGGAAAAA ATCTCAAGAA ACCAGCCCCC 
AAGTCTTACC TTATCCACGC TGGTCTGGAG CCCCTGACAT TCACCAATAT GTTTCCCAGC 
TGGGAGCACA GAGAGGACAT CGCTGAGATC ACAGAGATGG ACACGGAAGT TTCCAATCAG 
ATCACCCTCG TGGAAGACGT CTTAGCCAAG CTCTGTAAAA CCATTTACCC GCTGGCCGAC 
CTCCTGGCCA GGCCACTCCC GGAGGGGGTC GATCCTCTGA AGCTTGAGAT CTATCTCACC 
GACGAAGACT TCGAGTTTGC ACTAGACATG ACGAGGGATG AATACAACGC CCTGCCCGCC 
TGGAAGCAGG TGAACCTGAA GAAAGCAAAA GGCCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872071.1
CDS	1241..6862
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_017016582.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016582.1

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGGTGCACTT CACATTCAGA AACGCCAACT GTCGATGATG AAGAAAAGGT GGATGAACGA 
GCCAAGCTGA GCGTCGCCGC CAAGAGGTTG CTTTTCAGGG AGATGGAAAA ATCTTTTGAT 
GAACAAAATG TTCCAAAGCG ACGCTCAAGA AACACAGCTG TGGAGCAGAG GCTACGCCGT 
CTGCAGGACA GGTCCCTCAC CCAGCCCATC ACCACTGAAG AGGTGGTCAT CGCAGCCACA 
TTGCAGGCCT CTGCTCACCA AAAGGCCTTA GCCAAGGACC AGACAAATGA GGGCAAAGAG 
CTTGCTGAGC AAGGAGAACC TGATTCCTCC ACTCTAAGCT TGGCCGAAAA GTTGGCCTTG 
TTTAACAAAT TGTCCCAGCC AGTCTCAAAA GCGATTTCTA CCCGGAACAG AATAGACACG 
AGACAGAGGA GAATGAACGC TCGCTATCAA ACTCAGCCAG TCACACTGGG AGAGGTGGAG 
CAGGTGCAGA GTGGAAAGCT CATTCCTTTC TCACCTGCCG TGAACACATC AGTGTCTACC 
GTAGCATCCA CGGTTGCTCC AATGTATGCC GGAGATCTTC GCACAAAGCC ACCTCTTGAC 
CACAATGCAA GTGCCACTGA CTATAAGTTT TCTTCTTCAA TAGAAAATTC GGACTCTCCA 
GTTAGAAGCA TTCTGAAATC GCAAGCTTGG CAGCCTTTGG TAGAGGGTAG CGAGAACAAG 
GGAATGTTGA GAGAATATGG AGAGACAGAA AGCAAGAGAG CTTTGACAGG TCGAGACAGT 
GGGATGGAGA AGTATGGGTC CTTTGAGGAA GCAGAAGCAT CCTACCCCAT CCTGAACCGA 
GCCAGGGAAG GAGACAGCCA TAAGGAATCT AAATATGCTG TTCCCAGAAG AGGAAGCCTG 
GAACGGGCGA ACCCTCCCAT CACCCACCTC GGGGATGAAC CGAAGGAATT TTCCATGGCT 
AAAATGAATG CACAAGGAAA CTTGGACTTG AGGGACAGGC TGCCCTTTGA AGAGAAGGTG 
GAGGTGGAGA ATGTTATGAA AAGGAAGTTT TCACTAAGAG CGGCAGAGTT CGGGGAGCCC 
ACTTCCGAGC AGACGGGGAC AGCTGCTGGG AAAACTATTG CTCAAACCAC AGCCCCCGTG 
TCCTGGAAGC CCCAGGATTC TTCGGAACAG CCACAGGAGA AGCTCTGCAA GAATCCATGT 
GCGATGTTTG CTGCTGGAGA GATCAAAACG CCGACAGGGG AGGGCCTTCT TGACTCACCC 
AGCAAAACCA TGTCTATTAA AGAAAGATTG GCACTGTTGA AGAAAAGCGG GGAGGAAGAT 
TGGAGAAACA GACTCAGCAG GAGGCAGGAG GGCGGCAAGG CGCCGGCCAG CAGCCTGCAC 
ACCCAGGAAG CAGGGCGGTC CCTCATCAAG AAGCGGGTCA CAGAAAGTCG AGAGAGCCAA 
ATGACGATTG AGGAGAGGAA GCAGCTCATC ACTGTGAGAG AGGAGGCCTG GAAGACGAGA 
GGCAGAGGAG CGGCCAACGA CTCGACCCAG TTCACTGTGG CTGGCAGGAT GGTGAAGAAA 
GGTTTGGCGT CACCTACTGC CATAACCCCA GTAGCCTCAC CCATTTGCGG TAAAACAAGA 
GGCACCACAC CCGTTTCCAA ACCCCTGGAA GATATCGAAG CCAGACCAGA TATGCAGTTA 
GAATCGGACC TGAAGTTGGA CAGGCTGGAA ACCTTTCTAA GAAGGCTGAA TAACAAAGTT 
GGCGGGATGC ACGAAACGGT GCTCACTGTC ACCGGCAAAT CTGTGAAGGA GGTGATGAAG 
CCAGATGATG ATGAAACCTT TGCCAAATTT TACCGCAGCG TGGATTATAA TATGCCAAGA 
AGTCCTGTGG AGATGGATGA GGACTTCGAT GTCATTTTCG ATCCTTATGC ACCCAAATTG 
ACGTCTTCCG TGGCCGAGCA CAAGCGGGCA GTTAGGCCCA AGCGCCGGGT TCAGGCCTCC 
AAAAACCCCC TGAAAATGCT GGCGGCAAGA GAAGATCTCC TTCAGGAATA CACTGAGCAG 
AGATTAAACG TTGCCTTCAT GGAGTCAAAG CGGATGAAAG TAGAAAAGAT GTCTTCCAAC 
TCCAACTTCT CAGAAGTCAC CCTGGCGGGT TTAGCCAGTA AAGAAAACTT CAGCAACGTC 
AGCCTGCGGA GCGTCAACCT GACGGAACAG AACTCTAACA ACAGCGCCGT GCCCTACAAG 
AGGCTGATGC TGTTGCAGAT TAAAGGAAGA AGACATGTGC AGACCAGGCT GGTGGAACCT 
CGAGCTTCGG CGCTCAACAG TGGGGACTGC TTCCTCCTGC TCTCTCCCCA CTGCTGCTTC 
CTGTGGGTAG GAGAGTTTGC AAACGTCATA GAAAAGGCGA AGGCCTCAGA ACTTGCAACT 
TTAATTCAGA CAAAGAGGGA ACTTGGTTGT AGAGCTACTT ATATCCAAAC CATTGAAGAA 
GGAATTAATA CACACACTCA TGCAGCCAAA GACTTCTGGA AGCTTCTGGG TGGCCAAACC 
AGTTACCAAT CTGCTGGAGA CCCAAAAGAA GATGAACTCT ATGAAGCAGC CATAATAGAA 
ACTAACTGCA TTTACCGTCT CATGGATGAC AAACTTGTTC CTGATGACGA CTACTGGGGG 
AAAATTCCGA AGTGCTCCCT TCTGCAACCC AAAGAGGTAC TGGTGTTTGA TTTTGGTAGT 
GAAGTTTACG TATGGCATGG GAAAGAAGTC ACATTAGCAC AACGAAAAAT AGCATTTCAG 
CTGGCAAAGC ACTTATGGAA TGGAACCTTT GACTATGAGA ACTGTGACAT CAATCCCCTG 
GATCCTGGAG AATGCAATCC GCTTATCCCC AGAAAAGGAC AGGGGCGGCC CGACTGGGCG 
ATATTTGGGA GACTTACTGA ACACAATGAG ACGATTTTGT TCAAAGAGAA GTTTCTGGAT 
TGGACGGAAC TGAAGAGATC GAATGAGAAG AACCCCGGGG AACTTGCCCA GCACAAGGAA 
GACCCCAGGA CTGATGTCAA GGCATACGAT GTGACACGGA TGGTGTCCAT GCCCCAGACG 
ACAGCAGGCA CCATCCTGGA CGGAGTGAAC GTCGGCCGTG GCTATGGCCT GGTGGAAGGA 
CACGACAGGA GGCAGTTTGA GATCACCAGC GTTTCCGTGG ATGTCTGGCA CATCCTGGAA 
TTCGACTATA GCAGGCTCCC CAAACAAAGC ATCGGGCAGT TCCATGAGGG GGATGCCTAT 
GTGGTCAAGT GGAAGTTCAT GGTGAGCACG GCAGTGGGAA GTCGCCAGAA GGGAGAGCAC 
TCGGTGAGGG CAGCCGGCAA AGAGAAGTGC GTCTACTTCT TCTGGCAAGG CCGGCACTCC 
ACCGTGAGTG AGAAGGGCAC GTCGGCGCTG ATGACGGTGG AGCTGGACGA GGAAAGGGGG 
GCCCAGGTCC AGGTTCTCCA GGGAAAGGAG CCCCCCTGTT TCCTGCAGTG TTTCCAGGGG 
GGGATGGTGG TGCACTCGGG GAGGCGGGAA GAGGAAGAAG AAAATGTGCA AAGTGAGTGG 
CGGCTGTACT GCGTGCGTGG AGAGGTGCCC GTGGAAGGGA ATTTGCTGGA AGTGGCCTGT 
CACTGTAGCA GCCTGAGGTC CAGAACTTCC ATGGTGGTGC TTAACGTCAA CAAGGCCCTC 
ATCTACCTGT GGCACGGATG CAAAGCCCAG GCCCACACGA AGGAGGTCGG AAGGACCGCT 
GCGAACAAGA TCAAGGAACA ATGTCCCCTG GAAGCAGGAC TGCATAGTAG CAGCAAAGTC 
ACAATACACG AGTGTGATGA AGGCTCCGAG CCACTCGGAT TCTGGGATGC CTTAGGAAGG 
AGAGACAGGA AAGCCTACGA TTGCATGCTT CAAGATCCTG GAAGTTTTAA CTTCGCGCCC 
CGCCTGTTCA TCCTCAGCAG CTCCTCTGGG GATTTTGCAG CCACAGAGTT TGTGTACCCT 
GCCCGAGCCC CCTCTGTGGT CAGTTCCATG CCCTTCCTGC AGGAAGATCT GTACAGCGCG 
CCCCAGCCAG CACTTTTCCT TGTTGACAAT CACCACGAGG TGTACCTCTG GCAAGGCTGG 
TGGCCCATCG AGAACAAGAT CACTGGTTCC GCCCGCATCC GCTGGGCCTC CGACCGGAAG 
AGTGCGATGG AGACTGTGCT CCAGTACTGC AAAGGAAAAA ATCTCAAGAA ACCAGCCCCC 
AAGTCTTACC TTATCCACGC TGGTCTGGAG CCCCTGACAT TCACCAATAT GTTTCCCAGC 
TGGGAGCACA GAGAGGACAT CGCTGAGATC ACAGAGATGG ACACGGAAGT TTCCAATCAG 
ATCACCCTCG TGGAAGACGT CTTAGCCAAG CTCTGTAAAA CCATTTACCC GCTGGCCGAC 
CTCCTGGCCA GGCCACTCCC GGAGGGGGTC GATCCTCTGA AGCTTGAGAT CTATCTCACC 
GACGAAGACT TCGAGTTTGC ACTAGACATG ACGAGGGATG AATACAACGC CCTGCCCGCC 
TGGAAGCAGG TGAACCTGAA GAAAGCAAAA GGCCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( NM_003174.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003165.2 Homo sapiens supervillin (SVIL), transcript variant 1, mRNA.

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CloneID	OHu18368
Clone ID Related Accession (Same CDS sequence)	NM_003174.3
Accession Version	NM_003174.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	supervillin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF051850.1, AL160060.11, AF051851.1, BX457194.2, BC092440.1 and AI028650.1. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2007 this sequence version replaced NM_003174.2. Transcript Variant: This variant (1) is alternatively spliced in the 5' portion of the gene, resulting in the shorter isoform (1) which has a different amino-terminus compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF051850.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGGTG CACTTCACAT 
TCAGAAACGC CAACTGTCGA TGATGAAGAA AAGGTGGATG AACGAGCCAA GCTGAGCGTC 
GCCGCCAAGA GGTTGCTTTT CAGGGAGATG GAAAAATCTT TTGATGAACA AAATGTTCCA 
AAGCGACGCT CAAGAAACAC AGCTGTGGAG CAGAGGCTAC GCCGTCTGCA GGACAGGTCC 
CTCACCCAGC CCATCACCAC TGAAGAGGTG GTCATCGCAG CCACATTGCA GGCCTCTGCT 
CACCAAAAGG CCTTAGCCAA GGACCAGACA AATGAGGGCA AAGAGCTTGC TGAGCAAGGA 
GAACCTGATT CCTCCACTCT AAGCTTGGCC GAAAAGTTGG CCTTGTTTAA CAAATTGTCC 
CAGCCAGTCT CAAAAGCGAT TTCTACCCGG AACAGAATAG ACACGAGACA GAGGAGAATG 
AACGCTCGCT ATCAAACTCA GCCAGTCACA CTGGGAGAGG TGGAGCAGGT GCAGAGTGGA 
AAGCTCATTC CTTTCTCACC TGCCGTGAAC ACATCAGTGT CTACCGTAGC ATCCACGGTT 
GCTCCAATGT ATGCCGGAGA TCTTCGCACA AAGCCACCTC TTGACCACAA TGCAAGTGCC 
ACTGACTATA AGTTTTCTTC TTCAATAGAA AATTCGGACT CTCCAGTTAG AAGCATTCTG 
AAATCGCAAG CTTGGCAGCC TTTGGTAGAG GGTAGCGAGA ACAAGGGAAT GTTGAGAGAA 
TATGGAGAGA CAGAAAGCAA GAGAGCTTTG ACAGGTCGAG ACAGTGGGAT GGAGAAGTAT 
GGGTCCTTTG AGGAAGCAGA AGCATCCTAC CCCATCCTGA ACCGAGCCAG GGAAGGAGAC 
AGCCATAAGG AATCTAAATA TGCTGTTCCC AGAAGAGGAA GCCTGGAACG GGCGAACCCT 
CCCATCACCC ACCTCGGGGA TGAACCGAAG GAATTTTCCA TGGCTAAAAT GAATGCACAA 
GGAAACTTGG ACTTGAGGGA CAGGCTGCCC TTTGAAGAGA AGGTGGAGGT GGAGAATGTT 
ATGAAAAGGA AGTTTTCACT AAGAGCGGCA GAGTTCGGGG AGCCCACTTC CGAGCAGACG 
GGGACAGCTG CTGGGAAAAC TATTGCTCAA ACCACAGCCC CCGTGTCCTG GAAGCCCCAG 
GATTCTTCGG AACAGCCACA GGAGAAGCTC TGCAAGAATC CATGTGCGAT GTTTGCTGCT 
GGAGAGATCA AAACGCCGAC AGGGGAGGGC CTTCTTGACT CACCCAGCAA AACCATGTCT 
ATTAAAGAAA GATTGGCACT GTTGAAGAAA AGCGGGGAGG AAGATTGGAG AAACAGACTC 
AGCAGGAGGC AGGAGGGCGG CAAGGCGCCG GCCAGCAGCC TGCACACCCA GGAAGCAGGG 
CGGTCCCTCA TCAAGAAGCG GGTCACAGAA AGTCGAGAGA GCCAAATGAC GATTGAGGAG 
AGGAAGCAGC TCATCACTGT GAGAGAGGAG GCCTGGAAGA CGAGAGGCAG AGGAGCGGCC 
AACGACTCGA CCCAGTTCAC TGTGGCTGGC AGGATGGTGA AGAAAGGTTT GGCGTCACCT 
ACTGCCATAA CCCCAGTAGC CTCACCCATT TGCGGTAAAA CAAGAGGCAC CACACCCGTT 
TCCAAACCCC TGGAAGATAT CGAAGCCAGA CCAGATATGC AGTTAGAATC GGACCTGAAG 
TTGGACAGGC TGGAAACCTT TCTAAGAAGG CTGAATAACA AAGTTGGCGG GATGCACGAA 
ACGGTGCTCA CTGTCACCGG CAAATCTGTG AAGGAGGTGA TGAAGCCAGA TGATGATGAA 
ACCTTTGCCA AATTTTACCG CAGCGTGGAT TATAATATGC CAAGAAGTCC TGTGGAGATG 
GATGAGGACT TCGATGTCAT TTTCGATCCT TATGCACCCA AATTGACGTC TTCCGTGGCC 
GAGCACAAGC GGGCAGTTAG GCCCAAGCGC CGGGTTCAGG CCTCCAAAAA CCCCCTGAAA 
ATGCTGGCGG CAAGAGAAGA TCTCCTTCAG GAATACACTG AGCAGAGATT AAACGTTGCC 
TTCATGGAGT CAAAGCGGAT GAAAGTAGAA AAGATGTCTT CCAACTCCAA CTTCTCAGAA 
GTCACCCTGG CGGGTTTAGC CAGTAAAGAA AACTTCAGCA ACGTCAGCCT GCGGAGCGTC 
AACCTGACGG AACAGAACTC TAACAACAGC GCCGTGCCCT ACAAGAGGCT GATGCTGTTG 
CAGATTAAAG GAAGAAGACA TGTGCAGACC AGGCTGGTGG AACCTCGAGC TTCGGCGCTC 
AACAGTGGGG ACTGCTTCCT CCTGCTCTCT CCCCACTGCT GCTTCCTGTG GGTAGGAGAG 
TTTGCAAACG TCATAGAAAA GGCGAAGGCC TCAGAACTTG CAACTTTAAT TCAGACAAAG 
AGGGAACTTG GTTGTAGAGC TACTTATATC CAAACCATTG AAGAAGGAAT TAATACACAC 
ACTCATGCAG CCAAAGACTT CTGGAAGCTT CTGGGTGGCC AAACCAGTTA CCAATCTGCT 
GGAGACCCAA AAGAAGATGA ACTCTATGAA GCAGCCATAA TAGAAACTAA CTGCATTTAC 
CGTCTCATGG ATGACAAACT TGTTCCTGAT GACGACTACT GGGGGAAAAT TCCGAAGTGC 
TCCCTTCTGC AACCCAAAGA GGTACTGGTG TTTGATTTTG GTAGTGAAGT TTACGTATGG 
CATGGGAAAG AAGTCACATT AGCACAACGA AAAATAGCAT TTCAGCTGGC AAAGCACTTA 
TGGAATGGAA CCTTTGACTA TGAGAACTGT GACATCAATC CCCTGGATCC TGGAGAATGC 
AATCCGCTTA TCCCCAGAAA AGGACAGGGG CGGCCCGACT GGGCGATATT TGGGAGACTT 
ACTGAACACA ATGAGACGAT TTTGTTCAAA GAGAAGTTTC TGGATTGGAC GGAACTGAAG 
AGATCGAATG AGAAGAACCC CGGGGAACTT GCCCAGCACA AGGAAGACCC CAGGACTGAT 
GTCAAGGCAT ACGATGTGAC ACGGATGGTG TCCATGCCCC AGACGACAGC AGGCACCATC 
CTGGACGGAG TGAACGTCGG CCGTGGCTAT GGCCTGGTGG AAGGACACGA CAGGAGGCAG 
TTTGAGATCA CCAGCGTTTC CGTGGATGTC TGGCACATCC TGGAATTCGA CTATAGCAGG 
CTCCCCAAAC AAAGCATCGG GCAGTTCCAT GAGGGGGATG CCTATGTGGT CAAGTGGAAG 
TTCATGGTGA GCACGGCAGT GGGAAGTCGC CAGAAGGGAG AGCACTCGGT GAGGGCAGCC 
GGCAAAGAGA AGTGCGTCTA CTTCTTCTGG CAAGGCCGGC ACTCCACCGT GAGTGAGAAG 
GGCACGTCGG CGCTGATGAC GGTGGAGCTG GACGAGGAAA GGGGGGCCCA GGTCCAGGTT 
CTCCAGGGAA AGGAGCCCCC CTGTTTCCTG CAGTGTTTCC AGGGGGGGAT GGTGGTGCAC 
TCGGGGAGGC GGGAAGAGGA AGAAGAAAAT GTGCAAAGTG AGTGGCGGCT GTACTGCGTG 
CGTGGAGAGG TGCCCGTGGA AGGGAATTTG CTGGAAGTGG CCTGTCACTG TAGCAGCCTG 
AGGTCCAGAA CTTCCATGGT GGTGCTTAAC GTCAACAAGG CCCTCATCTA CCTGTGGCAC 
GGATGCAAAG CCCAGGCCCA CACGAAGGAG GTCGGAAGGA CCGCTGCGAA CAAGATCAAG 
GAACAATGTC CCCTGGAAGC AGGACTGCAT AGTAGCAGCA AAGTCACAAT ACACGAGTGT 
GATGAAGGCT CCGAGCCACT CGGATTCTGG GATGCCTTAG GAAGGAGAGA CAGGAAAGCC 
TACGATTGCA TGCTTCAAGA TCCTGGAAGT TTTAACTTCG CGCCCCGCCT GTTCATCCTC 
AGCAGCTCCT CTGGGGATTT TGCAGCCACA GAGTTTGTGT ACCCTGCCCG AGCCCCCTCT 
GTGGTCAGTT CCATGCCCTT CCTGCAGGAA GATCTGTACA GCGCGCCCCA GCCAGCACTT 
TTCCTTGTTG ACAATCACCA CGAGGTGTAC CTCTGGCAAG GCTGGTGGCC CATCGAGAAC 
AAGATCACTG GTTCCGCCCG CATCCGCTGG GCCTCCGACC GGAAGAGTGC GATGGAGACT 
GTGCTCCAGT ACTGCAAAGG AAAAAATCTC AAGAAACCAG CCCCCAAGTC TTACCTTATC 
CACGCTGGTC TGGAGCCCCT GACATTCACC AATATGTTTC CCAGCTGGGA GCACAGAGAG 
GACATCGCTG AGATCACAGA GATGGACACG GAAGTTTCCA ATCAGATCAC CCTCGTGGAA 
GACGTCTTAG CCAAGCTCTG TAAAACCATT TACCCGCTGG CCGACCTCCT GGCCAGGCCA 
CTCCCGGAGG GGGTCGATCC TCTGAAGCTT GAGATCTATC TCACCGACGA AGACTTCGAG 
TTTGCACTAG ACATGACGAG GGATGAATAC AACGCCCTGC CCGCCTGGAA GCAGGTGAAC 
CTGAAGAAAG CAAAAGGCCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003165.2
CDS	451..5817
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	NM_003174.3
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003174.3

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGGTG CACTTCACAT 
TCAGAAACGC CAACTGTCGA TGATGAAGAA AAGGTGGATG AACGAGCCAA GCTGAGCGTC 
GCCGCCAAGA GGTTGCTTTT CAGGGAGATG GAAAAATCTT TTGATGAACA AAATGTTCCA 
AAGCGACGCT CAAGAAACAC AGCTGTGGAG CAGAGGCTAC GCCGTCTGCA GGACAGGTCC 
CTCACCCAGC CCATCACCAC TGAAGAGGTG GTCATCGCAG CCACATTGCA GGCCTCTGCT 
CACCAAAAGG CCTTAGCCAA GGACCAGACA AATGAGGGCA AAGAGCTTGC TGAGCAAGGA 
GAACCTGATT CCTCCACTCT AAGCTTGGCC GAAAAGTTGG CCTTGTTTAA CAAATTGTCC 
CAGCCAGTCT CAAAAGCGAT TTCTACCCGG AACAGAATAG ACACGAGACA GAGGAGAATG 
AACGCTCGCT ATCAAACTCA GCCAGTCACA CTGGGAGAGG TGGAGCAGGT GCAGAGTGGA 
AAGCTCATTC CTTTCTCACC TGCCGTGAAC ACATCAGTGT CTACCGTAGC ATCCACGGTT 
GCTCCAATGT ATGCCGGAGA TCTTCGCACA AAGCCACCTC TTGACCACAA TGCAAGTGCC 
ACTGACTATA AGTTTTCTTC TTCAATAGAA AATTCGGACT CTCCAGTTAG AAGCATTCTG 
AAATCGCAAG CTTGGCAGCC TTTGGTAGAG GGTAGCGAGA ACAAGGGAAT GTTGAGAGAA 
TATGGAGAGA CAGAAAGCAA GAGAGCTTTG ACAGGTCGAG ACAGTGGGAT GGAGAAGTAT 
GGGTCCTTTG AGGAAGCAGA AGCATCCTAC CCCATCCTGA ACCGAGCCAG GGAAGGAGAC 
AGCCATAAGG AATCTAAATA TGCTGTTCCC AGAAGAGGAA GCCTGGAACG GGCGAACCCT 
CCCATCACCC ACCTCGGGGA TGAACCGAAG GAATTTTCCA TGGCTAAAAT GAATGCACAA 
GGAAACTTGG ACTTGAGGGA CAGGCTGCCC TTTGAAGAGA AGGTGGAGGT GGAGAATGTT 
ATGAAAAGGA AGTTTTCACT AAGAGCGGCA GAGTTCGGGG AGCCCACTTC CGAGCAGACG 
GGGACAGCTG CTGGGAAAAC TATTGCTCAA ACCACAGCCC CCGTGTCCTG GAAGCCCCAG 
GATTCTTCGG AACAGCCACA GGAGAAGCTC TGCAAGAATC CATGTGCGAT GTTTGCTGCT 
GGAGAGATCA AAACGCCGAC AGGGGAGGGC CTTCTTGACT CACCCAGCAA AACCATGTCT 
ATTAAAGAAA GATTGGCACT GTTGAAGAAA AGCGGGGAGG AAGATTGGAG AAACAGACTC 
AGCAGGAGGC AGGAGGGCGG CAAGGCGCCG GCCAGCAGCC TGCACACCCA GGAAGCAGGG 
CGGTCCCTCA TCAAGAAGCG GGTCACAGAA AGTCGAGAGA GCCAAATGAC GATTGAGGAG 
AGGAAGCAGC TCATCACTGT GAGAGAGGAG GCCTGGAAGA CGAGAGGCAG AGGAGCGGCC 
AACGACTCGA CCCAGTTCAC TGTGGCTGGC AGGATGGTGA AGAAAGGTTT GGCGTCACCT 
ACTGCCATAA CCCCAGTAGC CTCACCCATT TGCGGTAAAA CAAGAGGCAC CACACCCGTT 
TCCAAACCCC TGGAAGATAT CGAAGCCAGA CCAGATATGC AGTTAGAATC GGACCTGAAG 
TTGGACAGGC TGGAAACCTT TCTAAGAAGG CTGAATAACA AAGTTGGCGG GATGCACGAA 
ACGGTGCTCA CTGTCACCGG CAAATCTGTG AAGGAGGTGA TGAAGCCAGA TGATGATGAA 
ACCTTTGCCA AATTTTACCG CAGCGTGGAT TATAATATGC CAAGAAGTCC TGTGGAGATG 
GATGAGGACT TCGATGTCAT TTTCGATCCT TATGCACCCA AATTGACGTC TTCCGTGGCC 
GAGCACAAGC GGGCAGTTAG GCCCAAGCGC CGGGTTCAGG CCTCCAAAAA CCCCCTGAAA 
ATGCTGGCGG CAAGAGAAGA TCTCCTTCAG GAATACACTG AGCAGAGATT AAACGTTGCC 
TTCATGGAGT CAAAGCGGAT GAAAGTAGAA AAGATGTCTT CCAACTCCAA CTTCTCAGAA 
GTCACCCTGG CGGGTTTAGC CAGTAAAGAA AACTTCAGCA ACGTCAGCCT GCGGAGCGTC 
AACCTGACGG AACAGAACTC TAACAACAGC GCCGTGCCCT ACAAGAGGCT GATGCTGTTG 
CAGATTAAAG GAAGAAGACA TGTGCAGACC AGGCTGGTGG AACCTCGAGC TTCGGCGCTC 
AACAGTGGGG ACTGCTTCCT CCTGCTCTCT CCCCACTGCT GCTTCCTGTG GGTAGGAGAG 
TTTGCAAACG TCATAGAAAA GGCGAAGGCC TCAGAACTTG CAACTTTAAT TCAGACAAAG 
AGGGAACTTG GTTGTAGAGC TACTTATATC CAAACCATTG AAGAAGGAAT TAATACACAC 
ACTCATGCAG CCAAAGACTT CTGGAAGCTT CTGGGTGGCC AAACCAGTTA CCAATCTGCT 
GGAGACCCAA AAGAAGATGA ACTCTATGAA GCAGCCATAA TAGAAACTAA CTGCATTTAC 
CGTCTCATGG ATGACAAACT TGTTCCTGAT GACGACTACT GGGGGAAAAT TCCGAAGTGC 
TCCCTTCTGC AACCCAAAGA GGTACTGGTG TTTGATTTTG GTAGTGAAGT TTACGTATGG 
CATGGGAAAG AAGTCACATT AGCACAACGA AAAATAGCAT TTCAGCTGGC AAAGCACTTA 
TGGAATGGAA CCTTTGACTA TGAGAACTGT GACATCAATC CCCTGGATCC TGGAGAATGC 
AATCCGCTTA TCCCCAGAAA AGGACAGGGG CGGCCCGACT GGGCGATATT TGGGAGACTT 
ACTGAACACA ATGAGACGAT TTTGTTCAAA GAGAAGTTTC TGGATTGGAC GGAACTGAAG 
AGATCGAATG AGAAGAACCC CGGGGAACTT GCCCAGCACA AGGAAGACCC CAGGACTGAT 
GTCAAGGCAT ACGATGTGAC ACGGATGGTG TCCATGCCCC AGACGACAGC AGGCACCATC 
CTGGACGGAG TGAACGTCGG CCGTGGCTAT GGCCTGGTGG AAGGACACGA CAGGAGGCAG 
TTTGAGATCA CCAGCGTTTC CGTGGATGTC TGGCACATCC TGGAATTCGA CTATAGCAGG 
CTCCCCAAAC AAAGCATCGG GCAGTTCCAT GAGGGGGATG CCTATGTGGT CAAGTGGAAG 
TTCATGGTGA GCACGGCAGT GGGAAGTCGC CAGAAGGGAG AGCACTCGGT GAGGGCAGCC 
GGCAAAGAGA AGTGCGTCTA CTTCTTCTGG CAAGGCCGGC ACTCCACCGT GAGTGAGAAG 
GGCACGTCGG CGCTGATGAC GGTGGAGCTG GACGAGGAAA GGGGGGCCCA GGTCCAGGTT 
CTCCAGGGAA AGGAGCCCCC CTGTTTCCTG CAGTGTTTCC AGGGGGGGAT GGTGGTGCAC 
TCGGGGAGGC GGGAAGAGGA AGAAGAAAAT GTGCAAAGTG AGTGGCGGCT GTACTGCGTG 
CGTGGAGAGG TGCCCGTGGA AGGGAATTTG CTGGAAGTGG CCTGTCACTG TAGCAGCCTG 
AGGTCCAGAA CTTCCATGGT GGTGCTTAAC GTCAACAAGG CCCTCATCTA CCTGTGGCAC 
GGATGCAAAG CCCAGGCCCA CACGAAGGAG GTCGGAAGGA CCGCTGCGAA CAAGATCAAG 
GAACAATGTC CCCTGGAAGC AGGACTGCAT AGTAGCAGCA AAGTCACAAT ACACGAGTGT 
GATGAAGGCT CCGAGCCACT CGGATTCTGG GATGCCTTAG GAAGGAGAGA CAGGAAAGCC 
TACGATTGCA TGCTTCAAGA TCCTGGAAGT TTTAACTTCG CGCCCCGCCT GTTCATCCTC 
AGCAGCTCCT CTGGGGATTT TGCAGCCACA GAGTTTGTGT ACCCTGCCCG AGCCCCCTCT 
GTGGTCAGTT CCATGCCCTT CCTGCAGGAA GATCTGTACA GCGCGCCCCA GCCAGCACTT 
TTCCTTGTTG ACAATCACCA CGAGGTGTAC CTCTGGCAAG GCTGGTGGCC CATCGAGAAC 
AAGATCACTG GTTCCGCCCG CATCCGCTGG GCCTCCGACC GGAAGAGTGC GATGGAGACT 
GTGCTCCAGT ACTGCAAAGG AAAAAATCTC AAGAAACCAG CCCCCAAGTC TTACCTTATC 
CACGCTGGTC TGGAGCCCCT GACATTCACC AATATGTTTC CCAGCTGGGA GCACAGAGAG 
GACATCGCTG AGATCACAGA GATGGACACG GAAGTTTCCA ATCAGATCAC CCTCGTGGAA 
GACGTCTTAG CCAAGCTCTG TAAAACCATT TACCCGCTGG CCGACCTCCT GGCCAGGCCA 
CTCCCGGAGG GGGTCGATCC TCTGAAGCTT GAGATCTATC TCACCGACGA AGACTTCGAG 
TTTGCACTAG ACATGACGAG GGATGAATAC AACGCCCTGC CCGCCTGGAA GCAGGTGAAC 
CTGAAGAAAG CAAAAGGCCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448138.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303906.1 Homo sapiens supervillin (SVIL), transcript variant X1, mRNA.

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CloneID	OHu106555
Clone ID Related Accession (Same CDS sequence)	XM_024448138.1
Accession Version	XM_024448138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCAA ACGTATTCGG GAGAGAAAAG TCAGATTTCA ACAAGAAAAA CTTAAGCTTC 
CTTGGATTCC CACGTCAAAG GAAAGTTTCA AGCTTTCAGA AGGAGTTCTC ACTCGAAGAT 
AAAGAACAGC TCGCTAACCA CGAAAGAGGA ATCGATGCTC AGCTTTTAGT TGCACTTCCT 
AAAGTTGCAG AATTAAGACA AATCTTTGAA CCAAAGAAGA AAGAATTCTT AGAAATGAAA 
AGAAAAGAAA GAATTGCCAG GCGCCTGGAA GGGATTGAAA ATGACACTCA GCCCATCCTC 
TTGCAGAGCT GCACAGGATT GGTGACTCAC CGCCTGCTGG AGGAAGACAC CCCTCGATAC 
ATGAGAGCCA GCGACCCTGC CAGCCCCCAC ATCGGCCGAT CAAATGAAGA GGAGGAAACT 
TCTGATTCTT CTCTAGAAAA GCAAACTCGA TCCAAATACT GCACAGAAAC CTCCGGTGTC 
CACGGTGACT CACCCTATGG TTCGGGTACC ATGGACACCC ACAGTCTGGA GTCCAAAGCC 
GAAAGAATTG CAAGGTACAA AGCAGAAAGA AGGCGACAGC TGGCAGAGAA GTATGGGCTG 
ACTCTGGATC CCGAGGCCGA CTCCGAGTAT TTATCCCGCT ATACCAAGTC CAGGAAGGAG 
CCTGATGCTG TCGAGAAGCG GGGAGGAAAA AGTGACAAAC AGGAAGAGTC AAGCAGAGAT 
GCTAGTTCTC TGTACCCCGG GACCGAGACG ATGGGGCTCA GGACCTGTGC CGGTGAATCC 
AAGGACTATG CCCTCCATGT GGGTGACGGC TCTTCCGACC CGGAGGTGCT GCTGAACATA 
GAAAACCAAA GACGAGGTCA AGAGCTGAGT GCCACCCGGC AGGCCCATGA CCTGTCCCCA 
GCAGCCGAGA GTTCCTCGAC CTTCTCTTTC TCTGGGCGAG ACTCCTCCTT CACTGAAGTG 
CCACGGTCCC CCAAGCACGC CCACAGCTCC TCCCTGCAGC AGGCAGCCTC CCGGAGCCCC 
TCCTTTGGTG ACCCACAGCT ATCCCCTGAG GCCCGACCCA GTACAGGGAA ACCCAAACAT 
GAGTGGTTTC TCCAGAAAGA TTCCGAAGGG GACACACCTT CACTTATCAA CTGGCCTTCC 
AGAGTTAAAG TTAGAGAAAA ATTGGTGAAA GAGGAAAGTG CTCGAAACAG CCCTGAACTC 
GCCTCAGAGT CCGTAACTCA GAGGAGACAC CAGCCAGCGC CAGTCCATTA CGTGTCATTT 
CAGTCTGAGC ACTCAGCCTT TGATAGGGTC CCCAGCAAGG CAGCAGGCTC TACACGACAG 
CCAATCCGTG GCTATGTCCA ACCCGCAGAT ACCGGTCACA CCGCCAAGCT AGTGACGCCA 
GAAACCCCAG AAAATGCATC TGAGTGTAGC TGGGTAGCAT CAGCCACCCA GAATGTCCCC 
AAACCTCCCA GCTTGACGGT TCTAGAAGGT GACGGAAGGG ATAGCCCAGT TCTCCATGTC 
TGCGAGTCAA AAGCAGAAGA AGAAGAAGGG GAAGGAGAAG GAGAAGAAAA AGAAGAAGAT 
GTGTGCTTCA CTGAAGCTCT CGAGCAAAGC AAGAAAACCC TACTGGCTTT GGAGGGTGAT 
GGGCTAGTGA GAAGCCCAGA AGATCCCTCT AGAAATGAGG ACTTTGGTAA GCCTGCTGTG 
AGCACAGTCA CCTTAGAGCA TCAGAAGGAA CTGGAAAACG TGGCACAACC CCCTCAAGCT 
CCGCACCAGC CCACTGAGAG GACAGGCAGG AGCGAGATGG TTCTCTACAT TCAAAGTGAG 
CCTGTGTCCC AAGACGCCAA ACCAACTGGT CACAACAGGG AAGCCTCGAA AAAGCGCAAG 
GTCCGTACCC GCTCTCTGTC AGATTTCACA GGCCCCCCTC AGCTCCAGGC CTTGAAGTAT 
AAGGACCCAG CTTCCAGGAG AGAGCTGGAG CTGCCCAGCT CCAAGACCGA AGGGCCTTAT 
GGGGAGATCA GCATGCTGGA CACAAAAGTC TCTGTCGCCC AGCTCCGAAG TGCGTTCCTG 
GCATCTGCCA ACGCCTGCAG GAGACCTGAA CTCAAATCAC GGGTGGAGAG GTCGGCTGAA 
GGACCTGGCT TGCCCACCGG TGTGGAACGG GAGAGAGGGT CCCGGAAACC AAGACGCTAT 
TTTTCTCCTG GTGAAAGTAG AAAAACTTCC GAGAGATTTA GAACCCAACC TATAACTTCA 
GCAGAACGAA AGGAATCGGA TAGGTGCACT TCACATTCAG AAACGCCAAC TGTCGATGAT 
GAAGAAAAGG TGGATGAACG AGCCAAGCTG AGCGTCGCCG CCAAGAGGTT GCTTTTCAGG 
GAGATGGAAA AATCTTTTGA TGAACAAAAT GTTCCAAAGC GACGCTCAAG AAACACAGCT 
GTGGAGCAGA GGCTACGCCG TCTGCAGGAC AGGTCCCTCA CCCAGCCCAT CACCACTGAA 
GAGGTGGTCA TCGCAGCCAC TGAACCTATC CCCGCTTCGT GTTCTGGGGG CACCCACCCT 
GTAATGGCGA GACTTCCTAG CCCCACTGTA GCTAGGAGCG CTGTGCAGCC TGCCAGATTG 
CAGGCCTCTG CTCACCAAAA GGCCTTAGCC AAGGACCAGA CAAATGAGGG CAAAGAGCTT 
GCTGAGCAAG GAGAACCTGA TTCCTCCACT CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT 
AACAAATTGT CCCAGCCAGT CTCAAAAGCG ATTTCTACCC GGAACAGAAT AGACACGAGA 
CAGAGGAGAA TGAACGCTCG CTATCAAACT CAGCCAGTCA CACTGGGAGA GGTGGAGCAG 
GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA CCTGCCGTGA ACACATCAGT GTCTACCGTA 
GCATCCACGG TTGCTCCAAT GTATGCCGGA GATCTTCGCA CAAAGCCACC TCTTGACCAC 
AATGCAAGTG CCACTGACTA TAAGTTTTCT TCTTCAATAG AAAATTCGGA CTCTCCAGTT 
AGAAGCATTC TGAAATCGCA AGCTTGGCAG CCTTTGGTAG AGGGTAGCGA GAACAAGGGA 
ATGTTGAGAG AATATGGAGA GACAGAAAGC AAGAGAGCTT TGACAGGTCG AGACAGTGGG 
ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA GAAGCATCCT ACCCCATCCT GAACCGAGCC 
AGGGAAGGAG ACAGCCATAA GGAATCTAAA TATGCTGTTC CCAGAAGAGG AAGCCTGGAA 
CGGGCGAACC CTCCCATCAC CCACCTCGGG GATGAACCGA AGGAATTTTC CATGGCTAAA 
ATGAATGCAC AAGGAAACTT GGACTTGAGG GACAGGCTGC CCTTTGAAGA GAAGGTGGAG 
GTGGAGAATG TTATGAAAAG GAAGTTTTCA CTAAGAGCGG CAGAGTTCGG GGAGCCCACT 
TCCGAGCAGA CGGGGACAGC TGCTGGGAAA ACTATTGCTC AAACCACAGC CCCCGTGTCC 
TGGAAGCCCC AGGATTCTTC GGAACAGCCA CAGGAGAAGC TCTGCAAGAA TCCATGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAACGCCG ACAGGGGAGG GCCTTCTTGA CTCACCCAGC 
AAAACCATGT CTATTAAAGA AAGATTGGCA CTGTTGAAGA AAAGCGGGGA GGAAGATTGG 
AGAAACAGAC TCAGCAGGAG GCAGGAGGGC GGCAAGGCGC CGGCCAGCAG CCTGCACACC 
CAGGAAGCAG GGCGGTCCCT CATCAAGAAG GAAGAAGGAG GAGTTGCGGA TGATAGTGCC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAT GCTTCTACTT ATTCTCCTGC TATTCCTGCT 
GCCCATAAAT ACCTGTCTTT TGTGTCGATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303906.1
CDS	406..7410
Translation	MKPNVFGREKSDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448138.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448138.1

ORF Insert Sequence:

ATGAAGCCAA ACGTATTCGG GAGAGAAAAG TCAGATTTCA ACAAGAAAAA CTTAAGCTTC 
CTTGGATTCC CACGTCAAAG GAAAGTTTCA AGCTTTCAGA AGGAGTTCTC ACTCGAAGAT 
AAAGAACAGC TCGCTAACCA CGAAAGAGGA ATCGATGCTC AGCTTTTAGT TGCACTTCCT 
AAAGTTGCAG AATTAAGACA AATCTTTGAA CCAAAGAAGA AAGAATTCTT AGAAATGAAA 
AGAAAAGAAA GAATTGCCAG GCGCCTGGAA GGGATTGAAA ATGACACTCA GCCCATCCTC 
TTGCAGAGCT GCACAGGATT GGTGACTCAC CGCCTGCTGG AGGAAGACAC CCCTCGATAC 
ATGAGAGCCA GCGACCCTGC CAGCCCCCAC ATCGGCCGAT CAAATGAAGA GGAGGAAACT 
TCTGATTCTT CTCTAGAAAA GCAAACTCGA TCCAAATACT GCACAGAAAC CTCCGGTGTC 
CACGGTGACT CACCCTATGG TTCGGGTACC ATGGACACCC ACAGTCTGGA GTCCAAAGCC 
GAAAGAATTG CAAGGTACAA AGCAGAAAGA AGGCGACAGC TGGCAGAGAA GTATGGGCTG 
ACTCTGGATC CCGAGGCCGA CTCCGAGTAT TTATCCCGCT ATACCAAGTC CAGGAAGGAG 
CCTGATGCTG TCGAGAAGCG GGGAGGAAAA AGTGACAAAC AGGAAGAGTC AAGCAGAGAT 
GCTAGTTCTC TGTACCCCGG GACCGAGACG ATGGGGCTCA GGACCTGTGC CGGTGAATCC 
AAGGACTATG CCCTCCATGT GGGTGACGGC TCTTCCGACC CGGAGGTGCT GCTGAACATA 
GAAAACCAAA GACGAGGTCA AGAGCTGAGT GCCACCCGGC AGGCCCATGA CCTGTCCCCA 
GCAGCCGAGA GTTCCTCGAC CTTCTCTTTC TCTGGGCGAG ACTCCTCCTT CACTGAAGTG 
CCACGGTCCC CCAAGCACGC CCACAGCTCC TCCCTGCAGC AGGCAGCCTC CCGGAGCCCC 
TCCTTTGGTG ACCCACAGCT ATCCCCTGAG GCCCGACCCA GTACAGGGAA ACCCAAACAT 
GAGTGGTTTC TCCAGAAAGA TTCCGAAGGG GACACACCTT CACTTATCAA CTGGCCTTCC 
AGAGTTAAAG TTAGAGAAAA ATTGGTGAAA GAGGAAAGTG CTCGAAACAG CCCTGAACTC 
GCCTCAGAGT CCGTAACTCA GAGGAGACAC CAGCCAGCGC CAGTCCATTA CGTGTCATTT 
CAGTCTGAGC ACTCAGCCTT TGATAGGGTC CCCAGCAAGG CAGCAGGCTC TACACGACAG 
CCAATCCGTG GCTATGTCCA ACCCGCAGAT ACCGGTCACA CCGCCAAGCT AGTGACGCCA 
GAAACCCCAG AAAATGCATC TGAGTGTAGC TGGGTAGCAT CAGCCACCCA GAATGTCCCC 
AAACCTCCCA GCTTGACGGT TCTAGAAGGT GACGGAAGGG ATAGCCCAGT TCTCCATGTC 
TGCGAGTCAA AAGCAGAAGA AGAAGAAGGG GAAGGAGAAG GAGAAGAAAA AGAAGAAGAT 
GTGTGCTTCA CTGAAGCTCT CGAGCAAAGC AAGAAAACCC TACTGGCTTT GGAGGGTGAT 
GGGCTAGTGA GAAGCCCAGA AGATCCCTCT AGAAATGAGG ACTTTGGTAA GCCTGCTGTG 
AGCACAGTCA CCTTAGAGCA TCAGAAGGAA CTGGAAAACG TGGCACAACC CCCTCAAGCT 
CCGCACCAGC CCACTGAGAG GACAGGCAGG AGCGAGATGG TTCTCTACAT TCAAAGTGAG 
CCTGTGTCCC AAGACGCCAA ACCAACTGGT CACAACAGGG AAGCCTCGAA AAAGCGCAAG 
GTCCGTACCC GCTCTCTGTC AGATTTCACA GGCCCCCCTC AGCTCCAGGC CTTGAAGTAT 
AAGGACCCAG CTTCCAGGAG AGAGCTGGAG CTGCCCAGCT CCAAGACCGA AGGGCCTTAT 
GGGGAGATCA GCATGCTGGA CACAAAAGTC TCTGTCGCCC AGCTCCGAAG TGCGTTCCTG 
GCATCTGCCA ACGCCTGCAG GAGACCTGAA CTCAAATCAC GGGTGGAGAG GTCGGCTGAA 
GGACCTGGCT TGCCCACCGG TGTGGAACGG GAGAGAGGGT CCCGGAAACC AAGACGCTAT 
TTTTCTCCTG GTGAAAGTAG AAAAACTTCC GAGAGATTTA GAACCCAACC TATAACTTCA 
GCAGAACGAA AGGAATCGGA TAGGTGCACT TCACATTCAG AAACGCCAAC TGTCGATGAT 
GAAGAAAAGG TGGATGAACG AGCCAAGCTG AGCGTCGCCG CCAAGAGGTT GCTTTTCAGG 
GAGATGGAAA AATCTTTTGA TGAACAAAAT GTTCCAAAGC GACGCTCAAG AAACACAGCT 
GTGGAGCAGA GGCTACGCCG TCTGCAGGAC AGGTCCCTCA CCCAGCCCAT CACCACTGAA 
GAGGTGGTCA TCGCAGCCAC TGAACCTATC CCCGCTTCGT GTTCTGGGGG CACCCACCCT 
GTAATGGCGA GACTTCCTAG CCCCACTGTA GCTAGGAGCG CTGTGCAGCC TGCCAGATTG 
CAGGCCTCTG CTCACCAAAA GGCCTTAGCC AAGGACCAGA CAAATGAGGG CAAAGAGCTT 
GCTGAGCAAG GAGAACCTGA TTCCTCCACT CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT 
AACAAATTGT CCCAGCCAGT CTCAAAAGCG ATTTCTACCC GGAACAGAAT AGACACGAGA 
CAGAGGAGAA TGAACGCTCG CTATCAAACT CAGCCAGTCA CACTGGGAGA GGTGGAGCAG 
GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA CCTGCCGTGA ACACATCAGT GTCTACCGTA 
GCATCCACGG TTGCTCCAAT GTATGCCGGA GATCTTCGCA CAAAGCCACC TCTTGACCAC 
AATGCAAGTG CCACTGACTA TAAGTTTTCT TCTTCAATAG AAAATTCGGA CTCTCCAGTT 
AGAAGCATTC TGAAATCGCA AGCTTGGCAG CCTTTGGTAG AGGGTAGCGA GAACAAGGGA 
ATGTTGAGAG AATATGGAGA GACAGAAAGC AAGAGAGCTT TGACAGGTCG AGACAGTGGG 
ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA GAAGCATCCT ACCCCATCCT GAACCGAGCC 
AGGGAAGGAG ACAGCCATAA GGAATCTAAA TATGCTGTTC CCAGAAGAGG AAGCCTGGAA 
CGGGCGAACC CTCCCATCAC CCACCTCGGG GATGAACCGA AGGAATTTTC CATGGCTAAA 
ATGAATGCAC AAGGAAACTT GGACTTGAGG GACAGGCTGC CCTTTGAAGA GAAGGTGGAG 
GTGGAGAATG TTATGAAAAG GAAGTTTTCA CTAAGAGCGG CAGAGTTCGG GGAGCCCACT 
TCCGAGCAGA CGGGGACAGC TGCTGGGAAA ACTATTGCTC AAACCACAGC CCCCGTGTCC 
TGGAAGCCCC AGGATTCTTC GGAACAGCCA CAGGAGAAGC TCTGCAAGAA TCCATGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAACGCCG ACAGGGGAGG GCCTTCTTGA CTCACCCAGC 
AAAACCATGT CTATTAAAGA AAGATTGGCA CTGTTGAAGA AAAGCGGGGA GGAAGATTGG 
AGAAACAGAC TCAGCAGGAG GCAGGAGGGC GGCAAGGCGC CGGCCAGCAG CCTGCACACC 
CAGGAAGCAG GGCGGTCCCT CATCAAGAAG GAAGAAGGAG GAGTTGCGGA TGATAGTGCC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAT GCTTCTACTT ATTCTCCTGC TATTCCTGCT 
GCCCATAAAT ACCTGTCTTT TGTGTCGATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448140.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303908.1 Homo sapiens supervillin (SVIL), transcript variant X3, mRNA.

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CloneID	OHu106556
Clone ID Related Accession (Same CDS sequence)	XM_024448140.1
Accession Version	XM_024448140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGAGT TAAAGTTAGA 
GAAAAATTGG TGAAAGAGGA AAGTGCTCGA AACAGCCCTG AACTCGCCTC AGAGTCCGTA 
ACTCAGAGGA GACACCAGCC AGCGCCAGTC CATTACGTGT CATTTCAGTC TGAGCACTCA 
GCCTTTGATA GGGTCCCCAG CAAGGCAGCA GGCTCTACAC GACAGCCAAT CCGTGGCTAT 
GTCCAACCCG CAGATACCGG TCACACCGCC AAGCTAGTGA CGCCAGAAAC CCCAGAAAAT 
GCATCTGAGT GTAGCTGGGT AGCATCAGCC ACCCAGAATG TCCCCAAACC TCCCAGCTTG 
ACGGTTCTAG AAGGTGACGG AAGGGATAGC CCAGTTCTCC ATGTCTGCGA GTCAAAAGCA 
GAAGAAGAAG AAGGGGAAGG AGAAGGAGAA GAAAAAGAAG AAGATGTGTG CTTCACTGAA 
GCTCTCGAGC AAAGCAAGAA AACCCTACTG GCTTTGGAGG GTGATGGGCT AGTGAGAAGC 
CCAGAAGATC CCTCTAGAAA TGAGGACTTT GGTAAGCCTG CTGTGAGCAC AGTCACCTTA 
GAGCATCAGA AGGAACTGGA AAACGTGGCA CAACCCCCTC AAGCTCCGCA CCAGCCCACT 
GAGAGGACAG GCAGGAGCGA GATGGTTCTC TACATTCAAA GTGAGCCTGT GTCCCAAGAC 
GCCAAACCAA CTGGTCACAA CAGGGAAGCC TCGAAAAAGC GCAAGGTCCG TACCCGCTCT 
CTGTCAGATT TCACAGGCCC CCCTCAGCTC CAGGCCTTGA AGTATAAGGA CCCAGCTTCC 
AGGAGAGAGC TGGAGCTGCC CAGCTCCAAG ACCGAAGGGC CTTATGGGGA GATCAGCATG 
CTGGACACAA AAGTCTCTGT CGCCCAGCTC CGAAGTGCGT TCCTGGCATC TGCCAACGCC 
TGCAGGAGAC CTGAACTCAA ATCACGGGTG GAGAGGTCGG CTGAAGGACC TGGCTTGCCC 
ACCGGTGTGG AACGGGAGAG AGGGTCCCGG AAACCAAGAC GCTATTTTTC TCCTGGTGAA 
AGTAGAAAAA CTTCCGAGAG ATTTAGAACC CAACCTATAA CTTCAGCAGA ACGAAAGGAA 
TCGGATAGGT GCACTTCACA TTCAGAAACG CCAACTGTCG ATGATGAAGA AAAGGTGGAT 
GAACGAGCCA AGCTGAGCGT CGCCGCCAAG AGGTTGCTTT TCAGGGAGAT GGAAAAATCT 
TTTGATGAAC AAAATGTTCC AAAGCGACGC TCAAGAAACA CAGCTGTGGA GCAGAGGCTA 
CGCCGTCTGC AGGACAGGTC CCTCACCCAG CCCATCACCA CTGAAGAGGT GGTCATCGCA 
GCCACTGAAC CTATCCCCGC TTCGTGTTCT GGGGGCACCC ACCCTGTAAT GGCGAGACTT 
CCTAGCCCCA CTGTAGCTAG GAGCGCTGTG CAGCCTGCCA GATTGCAGGC CTCTGCTCAC 
CAAAAGGCCT TAGCCAAGGA CCAGACAAAT GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA 
CCTGATTCCT CCACTCTAAG CTTGGCCGAA AAGTTGGCCT TGTTTAACAA ATTGTCCCAG 
CCAGTCTCAA AAGCGATTTC TACCCGGAAC AGAATAGACA CGAGACAGAG GAGAATGAAC 
GCTCGCTATC AAACTCAGCC AGTCACACTG GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG 
CTCATTCCTT TCTCACCTGC CGTGAACACA TCAGTGTCTA CCGTAGCATC CACGGTTGCT 
CCAATGTATG CCGGAGATCT TCGCACAAAG CCACCTCTTG ACCACAATGC AAGTGCCACT 
GACTATAAGT TTTCTTCTTC AATAGAAAAT TCGGACTCTC CAGTTAGAAG CATTCTGAAA 
TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT AGCGAGAACA AGGGAATGTT GAGAGAATAT 
GGAGAGACAG AAAGCAAGAG AGCTTTGACA GGTCGAGACA GTGGGATGGA GAAGTATGGG 
TCCTTTGAGG AAGCAGAAGC ATCCTACCCC ATCCTGAACC GAGCCAGGGA AGGAGACAGC 
CATAAGGAAT CTAAATATGC TGTTCCCAGA AGAGGAAGCC TGGAACGGGC GAACCCTCCC 
ATCACCCACC TCGGGGATGA ACCGAAGGAA TTTTCCATGG CTAAAATGAA TGCACAAGGA 
AACTTGGACT TGAGGGACAG GCTGCCCTTT GAAGAGAAGG TGGAGGTGGA GAATGTTATG 
AAAAGGAAGT TTTCACTAAG AGCGGCAGAG TTCGGGGAGC CCACTTCCGA GCAGACGGGG 
ACAGCTGCTG GGAAAACTAT TGCTCAAACC ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT 
TCTTCGGAAC AGCCACAGGA GAAGCTCTGC AAGAATCCAT GTGCGATGTT TGCTGCTGGA 
GAGATCAAAA CGCCGACAGG GGAGGGCCTT CTTGACTCAC CCAGCAAAAC CATGTCTATT 
AAAGAAAGAT TGGCACTGTT GAAGAAAAGC GGGGAGGAAG ATTGGAGAAA CAGACTCAGC 
AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC AGCAGCCTGC ACACCCAGGA AGCAGGGCGG 
TCCCTCATCA AGAAGGAAGA AGGAGGAGTT GCGGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATTC CTGCTGCCCA TAAATACCTG 
TCTTTTGTGT CGATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAAAT GACGATTGAG 
GAGAGGAAGC AGCTCATCAC TGTGAGAGAG GAGGCCTGGA AGACGAGAGG CAGAGGAGCG 
GCCAACGACT CGACCCAGTT CACTGTGGCT GGCAGGATGG TGAAGAAAGG TTTGGCGTCA 
CCTACTGCCA TAACCCCAGT AGCCTCACCC ATTTGCGGTA AAACAAGAGG CACCACACCC 
GTTTCCAAAC CCCTGGAAGA TATCGAAGCC AGACCAGATA TGCAGTTAGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG CGGGATGCAC 
GAAACGGTGC TCACTGTCAC CGGCAAATCT GTGAAGGAGG TGATGAAGCC AGATGATGAT 
GAAACCTTTG CCAAATTTTA CCGCAGCGTG GATTATAATA TGCCAAGAAG TCCTGTGGAG 
ATGGATGAGG ACTTCGATGT CATTTTCGAT CCTTATGCAC CCAAATTGAC GTCTTCCGTG 
GCCGAGCACA AGCGGGCAGT TAGGCCCAAG CGCCGGGTTC AGGCCTCCAA AAACCCCCTG 
AAAATGCTGG CGGCAAGAGA AGATCTCCTT CAGGAATACA CTGAGCAGAG ATTAAACGTT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CTTCCAACTC CAACTTCTCA 
GAAGTCACCC TGGCGGGTTT AGCCAGTAAA GAAAACTTCA GCAACGTCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA CTCTAACAAC AGCGCCGTGC CCTACAAGAG GCTGATGCTG 
TTGCAGATTA AAGGAAGAAG ACATGTGCAG ACCAGGCTGG TGGAACCTCG AGCTTCGGCG 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCTCCCCACT GCTGCTTCCT GTGGGTAGGA 
GAGTTTGCAA ACGTCATAGA AAAGGCGAAG GCCTCAGAAC TTGCAACTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACTTAT ATCCAAACCA TTGAAGAAGG AATTAATACA 
CACACTCATG CAGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAATCT 
GCTGGAGACC CAAAAGAAGA TGAACTCTAT GAAGCAGCCA TAATAGAAAC TAACTGCATT 
TACCGTCTCA TGGATGACAA ACTTGTTCCT GATGACGACT ACTGGGGGAA AATTCCGAAG 
TGCTCCCTTC TGCAACCCAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTTTACGTA 
TGGCATGGGA AAGAAGTCAC ATTAGCACAA CGAAAAATAG CATTTCAGCT GGCAAAGCAC 
TTATGGAATG GAACCTTTGA CTATGAGAAC TGTGACATCA ATCCCCTGGA TCCTGGAGAA 
TGCAATCCGC TTATCCCCAG AAAAGGACAG GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA 
CTTACTGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAGT TTCTGGATTG GACGGAACTG 
AAGAGATCGA ATGAGAAGAA CCCCGGGGAA CTTGCCCAGC ACAAGGAAGA CCCCAGGACT 
GATGTCAAGG CATACGATGT GACACGGATG GTGTCCATGC CCCAGACGAC AGCAGGCACC 
ATCCTGGACG GAGTGAACGT CGGCCGTGGC TATGGCCTGG TGGAAGGACA CGACAGGAGG 
CAGTTTGAGA TCACCAGCGT TTCCGTGGAT GTCTGGCACA TCCTGGAATT CGACTATAGC 
AGGCTCCCCA AACAAAGCAT CGGGCAGTTC CATGAGGGGG ATGCCTATGT GGTCAAGTGG 
AAGTTCATGG TGAGCACGGC AGTGGGAAGT CGCCAGAAGG GAGAGCACTC GGTGAGGGCA 
GCCGGCAAAG AGAAGTGCGT CTACTTCTTC TGGCAAGGCC GGCACTCCAC CGTGAGTGAG 
AAGGGCACGT CGGCGCTGAT GACGGTGGAG CTGGACGAGG AAAGGGGGGC CCAGGTCCAG 
GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC CTGCAGTGTT TCCAGGGGGG GATGGTGGTG 
CACTCGGGGA GGCGGGAAGA GGAAGAAGAA AATGTGCAAA GTGAGTGGCG GCTGTACTGC 
GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC 
CTGAGGTCCA GAACTTCCAT GGTGGTGCTT AACGTCAACA AGGCCCTCAT CTACCTGTGG 
CACGGATGCA AAGCCCAGGC CCACACGAAG GAGGTCGGAA GGACCGCTGC GAACAAGATC 
AAGGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTCAC AATACACGAG 
TGTGATGAAG GCTCCGAGCC ACTCGGATTC TGGGATGCCT TAGGAAGGAG AGACAGGAAA 
GCCTACGATT GCATGCTTCA AGATCCTGGA AGTTTTAACT TCGCGCCCCG CCTGTTCATC 
CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC ACAGAGTTTG TGTACCCTGC CCGAGCCCCC 
TCTGTGGTCA GTTCCATGCC CTTCCTGCAG GAAGATCTGT ACAGCGCGCC CCAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTG TACCTCTGGC AAGGCTGGTG GCCCATCGAG 
AACAAGATCA CTGGTTCCGC CCGCATCCGC TGGGCCTCCG ACCGGAAGAG TGCGATGGAG 
ACTGTGCTCC AGTACTGCAA AGGAAAAAAT CTCAAGAAAC CAGCCCCCAA GTCTTACCTT 
ATCCACGCTG GTCTGGAGCC CCTGACATTC ACCAATATGT TTCCCAGCTG GGAGCACAGA 
GAGGACATCG CTGAGATCAC AGAGATGGAC ACGGAAGTTT CCAATCAGAT CACCCTCGTG 
GAAGACGTCT TAGCCAAGCT CTGTAAAACC ATTTACCCGC TGGCCGACCT CCTGGCCAGG 
CCACTCCCGG AGGGGGTCGA TCCTCTGAAG CTTGAGATCT ATCTCACCGA CGAAGACTTC 
GAGTTTGCAC TAGACATGAC GAGGGATGAA TACAACGCCC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303908.1
CDS	201..6890
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448140.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448140.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGAGT TAAAGTTAGA 
GAAAAATTGG TGAAAGAGGA AAGTGCTCGA AACAGCCCTG AACTCGCCTC AGAGTCCGTA 
ACTCAGAGGA GACACCAGCC AGCGCCAGTC CATTACGTGT CATTTCAGTC TGAGCACTCA 
GCCTTTGATA GGGTCCCCAG CAAGGCAGCA GGCTCTACAC GACAGCCAAT CCGTGGCTAT 
GTCCAACCCG CAGATACCGG TCACACCGCC AAGCTAGTGA CGCCAGAAAC CCCAGAAAAT 
GCATCTGAGT GTAGCTGGGT AGCATCAGCC ACCCAGAATG TCCCCAAACC TCCCAGCTTG 
ACGGTTCTAG AAGGTGACGG AAGGGATAGC CCAGTTCTCC ATGTCTGCGA GTCAAAAGCA 
GAAGAAGAAG AAGGGGAAGG AGAAGGAGAA GAAAAAGAAG AAGATGTGTG CTTCACTGAA 
GCTCTCGAGC AAAGCAAGAA AACCCTACTG GCTTTGGAGG GTGATGGGCT AGTGAGAAGC 
CCAGAAGATC CCTCTAGAAA TGAGGACTTT GGTAAGCCTG CTGTGAGCAC AGTCACCTTA 
GAGCATCAGA AGGAACTGGA AAACGTGGCA CAACCCCCTC AAGCTCCGCA CCAGCCCACT 
GAGAGGACAG GCAGGAGCGA GATGGTTCTC TACATTCAAA GTGAGCCTGT GTCCCAAGAC 
GCCAAACCAA CTGGTCACAA CAGGGAAGCC TCGAAAAAGC GCAAGGTCCG TACCCGCTCT 
CTGTCAGATT TCACAGGCCC CCCTCAGCTC CAGGCCTTGA AGTATAAGGA CCCAGCTTCC 
AGGAGAGAGC TGGAGCTGCC CAGCTCCAAG ACCGAAGGGC CTTATGGGGA GATCAGCATG 
CTGGACACAA AAGTCTCTGT CGCCCAGCTC CGAAGTGCGT TCCTGGCATC TGCCAACGCC 
TGCAGGAGAC CTGAACTCAA ATCACGGGTG GAGAGGTCGG CTGAAGGACC TGGCTTGCCC 
ACCGGTGTGG AACGGGAGAG AGGGTCCCGG AAACCAAGAC GCTATTTTTC TCCTGGTGAA 
AGTAGAAAAA CTTCCGAGAG ATTTAGAACC CAACCTATAA CTTCAGCAGA ACGAAAGGAA 
TCGGATAGGT GCACTTCACA TTCAGAAACG CCAACTGTCG ATGATGAAGA AAAGGTGGAT 
GAACGAGCCA AGCTGAGCGT CGCCGCCAAG AGGTTGCTTT TCAGGGAGAT GGAAAAATCT 
TTTGATGAAC AAAATGTTCC AAAGCGACGC TCAAGAAACA CAGCTGTGGA GCAGAGGCTA 
CGCCGTCTGC AGGACAGGTC CCTCACCCAG CCCATCACCA CTGAAGAGGT GGTCATCGCA 
GCCACTGAAC CTATCCCCGC TTCGTGTTCT GGGGGCACCC ACCCTGTAAT GGCGAGACTT 
CCTAGCCCCA CTGTAGCTAG GAGCGCTGTG CAGCCTGCCA GATTGCAGGC CTCTGCTCAC 
CAAAAGGCCT TAGCCAAGGA CCAGACAAAT GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA 
CCTGATTCCT CCACTCTAAG CTTGGCCGAA AAGTTGGCCT TGTTTAACAA ATTGTCCCAG 
CCAGTCTCAA AAGCGATTTC TACCCGGAAC AGAATAGACA CGAGACAGAG GAGAATGAAC 
GCTCGCTATC AAACTCAGCC AGTCACACTG GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG 
CTCATTCCTT TCTCACCTGC CGTGAACACA TCAGTGTCTA CCGTAGCATC CACGGTTGCT 
CCAATGTATG CCGGAGATCT TCGCACAAAG CCACCTCTTG ACCACAATGC AAGTGCCACT 
GACTATAAGT TTTCTTCTTC AATAGAAAAT TCGGACTCTC CAGTTAGAAG CATTCTGAAA 
TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT AGCGAGAACA AGGGAATGTT GAGAGAATAT 
GGAGAGACAG AAAGCAAGAG AGCTTTGACA GGTCGAGACA GTGGGATGGA GAAGTATGGG 
TCCTTTGAGG AAGCAGAAGC ATCCTACCCC ATCCTGAACC GAGCCAGGGA AGGAGACAGC 
CATAAGGAAT CTAAATATGC TGTTCCCAGA AGAGGAAGCC TGGAACGGGC GAACCCTCCC 
ATCACCCACC TCGGGGATGA ACCGAAGGAA TTTTCCATGG CTAAAATGAA TGCACAAGGA 
AACTTGGACT TGAGGGACAG GCTGCCCTTT GAAGAGAAGG TGGAGGTGGA GAATGTTATG 
AAAAGGAAGT TTTCACTAAG AGCGGCAGAG TTCGGGGAGC CCACTTCCGA GCAGACGGGG 
ACAGCTGCTG GGAAAACTAT TGCTCAAACC ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT 
TCTTCGGAAC AGCCACAGGA GAAGCTCTGC AAGAATCCAT GTGCGATGTT TGCTGCTGGA 
GAGATCAAAA CGCCGACAGG GGAGGGCCTT CTTGACTCAC CCAGCAAAAC CATGTCTATT 
AAAGAAAGAT TGGCACTGTT GAAGAAAAGC GGGGAGGAAG ATTGGAGAAA CAGACTCAGC 
AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC AGCAGCCTGC ACACCCAGGA AGCAGGGCGG 
TCCCTCATCA AGAAGGAAGA AGGAGGAGTT GCGGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATTC CTGCTGCCCA TAAATACCTG 
TCTTTTGTGT CGATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAAAT GACGATTGAG 
GAGAGGAAGC AGCTCATCAC TGTGAGAGAG GAGGCCTGGA AGACGAGAGG CAGAGGAGCG 
GCCAACGACT CGACCCAGTT CACTGTGGCT GGCAGGATGG TGAAGAAAGG TTTGGCGTCA 
CCTACTGCCA TAACCCCAGT AGCCTCACCC ATTTGCGGTA AAACAAGAGG CACCACACCC 
GTTTCCAAAC CCCTGGAAGA TATCGAAGCC AGACCAGATA TGCAGTTAGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG CGGGATGCAC 
GAAACGGTGC TCACTGTCAC CGGCAAATCT GTGAAGGAGG TGATGAAGCC AGATGATGAT 
GAAACCTTTG CCAAATTTTA CCGCAGCGTG GATTATAATA TGCCAAGAAG TCCTGTGGAG 
ATGGATGAGG ACTTCGATGT CATTTTCGAT CCTTATGCAC CCAAATTGAC GTCTTCCGTG 
GCCGAGCACA AGCGGGCAGT TAGGCCCAAG CGCCGGGTTC AGGCCTCCAA AAACCCCCTG 
AAAATGCTGG CGGCAAGAGA AGATCTCCTT CAGGAATACA CTGAGCAGAG ATTAAACGTT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CTTCCAACTC CAACTTCTCA 
GAAGTCACCC TGGCGGGTTT AGCCAGTAAA GAAAACTTCA GCAACGTCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA CTCTAACAAC AGCGCCGTGC CCTACAAGAG GCTGATGCTG 
TTGCAGATTA AAGGAAGAAG ACATGTGCAG ACCAGGCTGG TGGAACCTCG AGCTTCGGCG 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCTCCCCACT GCTGCTTCCT GTGGGTAGGA 
GAGTTTGCAA ACGTCATAGA AAAGGCGAAG GCCTCAGAAC TTGCAACTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACTTAT ATCCAAACCA TTGAAGAAGG AATTAATACA 
CACACTCATG CAGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAATCT 
GCTGGAGACC CAAAAGAAGA TGAACTCTAT GAAGCAGCCA TAATAGAAAC TAACTGCATT 
TACCGTCTCA TGGATGACAA ACTTGTTCCT GATGACGACT ACTGGGGGAA AATTCCGAAG 
TGCTCCCTTC TGCAACCCAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTTTACGTA 
TGGCATGGGA AAGAAGTCAC ATTAGCACAA CGAAAAATAG CATTTCAGCT GGCAAAGCAC 
TTATGGAATG GAACCTTTGA CTATGAGAAC TGTGACATCA ATCCCCTGGA TCCTGGAGAA 
TGCAATCCGC TTATCCCCAG AAAAGGACAG GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA 
CTTACTGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAGT TTCTGGATTG GACGGAACTG 
AAGAGATCGA ATGAGAAGAA CCCCGGGGAA CTTGCCCAGC ACAAGGAAGA CCCCAGGACT 
GATGTCAAGG CATACGATGT GACACGGATG GTGTCCATGC CCCAGACGAC AGCAGGCACC 
ATCCTGGACG GAGTGAACGT CGGCCGTGGC TATGGCCTGG TGGAAGGACA CGACAGGAGG 
CAGTTTGAGA TCACCAGCGT TTCCGTGGAT GTCTGGCACA TCCTGGAATT CGACTATAGC 
AGGCTCCCCA AACAAAGCAT CGGGCAGTTC CATGAGGGGG ATGCCTATGT GGTCAAGTGG 
AAGTTCATGG TGAGCACGGC AGTGGGAAGT CGCCAGAAGG GAGAGCACTC GGTGAGGGCA 
GCCGGCAAAG AGAAGTGCGT CTACTTCTTC TGGCAAGGCC GGCACTCCAC CGTGAGTGAG 
AAGGGCACGT CGGCGCTGAT GACGGTGGAG CTGGACGAGG AAAGGGGGGC CCAGGTCCAG 
GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC CTGCAGTGTT TCCAGGGGGG GATGGTGGTG 
CACTCGGGGA GGCGGGAAGA GGAAGAAGAA AATGTGCAAA GTGAGTGGCG GCTGTACTGC 
GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC 
CTGAGGTCCA GAACTTCCAT GGTGGTGCTT AACGTCAACA AGGCCCTCAT CTACCTGTGG 
CACGGATGCA AAGCCCAGGC CCACACGAAG GAGGTCGGAA GGACCGCTGC GAACAAGATC 
AAGGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTCAC AATACACGAG 
TGTGATGAAG GCTCCGAGCC ACTCGGATTC TGGGATGCCT TAGGAAGGAG AGACAGGAAA 
GCCTACGATT GCATGCTTCA AGATCCTGGA AGTTTTAACT TCGCGCCCCG CCTGTTCATC 
CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC ACAGAGTTTG TGTACCCTGC CCGAGCCCCC 
TCTGTGGTCA GTTCCATGCC CTTCCTGCAG GAAGATCTGT ACAGCGCGCC CCAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTG TACCTCTGGC AAGGCTGGTG GCCCATCGAG 
AACAAGATCA CTGGTTCCGC CCGCATCCGC TGGGCCTCCG ACCGGAAGAG TGCGATGGAG 
ACTGTGCTCC AGTACTGCAA AGGAAAAAAT CTCAAGAAAC CAGCCCCCAA GTCTTACCTT 
ATCCACGCTG GTCTGGAGCC CCTGACATTC ACCAATATGT TTCCCAGCTG GGAGCACAGA 
GAGGACATCG CTGAGATCAC AGAGATGGAC ACGGAAGTTT CCAATCAGAT CACCCTCGTG 
GAAGACGTCT TAGCCAAGCT CTGTAAAACC ATTTACCCGC TGGCCGACCT CCTGGCCAGG 
CCACTCCCGG AGGGGGTCGA TCCTCTGAAG CTTGAGATCT ATCTCACCGA CGAAGACTTC 
GAGTTTGCAC TAGACATGAC GAGGGATGAA TACAACGCCC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448139.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303907.1 Homo sapiens supervillin (SVIL), transcript variant X2, mRNA.

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CloneID	OHu60822
Clone ID Related Accession (Same CDS sequence)	XM_024448139.1
Accession Version	XM_024448139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGCGGGTC ACAGAAAGTC GAGAGAGCCA AATGACGATT 
GAGGAGAGGA AGCAGCTCAT CACTGTGAGA GAGGAGGCCT GGAAGACGAG AGGCAGAGGA 
GCGGCCAACG ACTCGACCCA GTTCACTGTG GCTGGCAGGA TGGTGAAGAA AGGTTTGGCG 
TCACCTACTG CCATAACCCC AGTAGCCTCA CCCATTTGCG GTAAAACAAG AGGCACCACA 
CCCGTTTCCA AACCCCTGGA AGATATCGAA GCCAGACCAG ATATGCAGTT AGAATCGGAC 
CTGAAGTTGG ACAGGCTGGA AACCTTTCTA AGAAGGCTGA ATAACAAAGT TGGCGGGATG 
CACGAAACGG TGCTCACTGT CACCGGCAAA TCTGTGAAGG AGGTGATGAA GCCAGATGAT 
GATGAAACCT TTGCCAAATT TTACCGCAGC GTGGATTATA ATATGCCAAG AAGTCCTGTG 
GAGATGGATG AGGACTTCGA TGTCATTTTC GATCCTTATG CACCCAAATT GACGTCTTCC 
GTGGCCGAGC ACAAGCGGGC AGTTAGGCCC AAGCGCCGGG TTCAGGCCTC CAAAAACCCC 
CTGAAAATGC TGGCGGCAAG AGAAGATCTC CTTCAGGAAT ACACTGAGCA GAGATTAAAC 
GTTGCCTTCA TGGAGTCAAA GCGGATGAAA GTAGAAAAGA TGTCTTCCAA CTCCAACTTC 
TCAGAAGTCA CCCTGGCGGG TTTAGCCAGT AAAGAAAACT TCAGCAACGT CAGCCTGCGG 
AGCGTCAACC TGACGGAACA GAACTCTAAC AACAGCGCCG TGCCCTACAA GAGGCTGATG 
CTGTTGCAGA TTAAAGGAAG AAGACATGTG CAGACCAGGC TGGTGGAACC TCGAGCTTCG 
GCGCTCAACA GTGGGGACTG CTTCCTCCTG CTCTCTCCCC ACTGCTGCTT CCTGTGGGTA 
GGAGAGTTTG CAAACGTCAT AGAAAAGGCG AAGGCCTCAG AACTTGCAAC TTTAATTCAG 
ACAAAGAGGG AACTTGGTTG TAGAGCTACT TATATCCAAA CCATTGAAGA AGGAATTAAT 
ACACACACTC ATGCAGCCAA AGACTTCTGG AAGCTTCTGG GTGGCCAAAC CAGTTACCAA 
TCTGCTGGAG ACCCAAAAGA AGATGAACTC TATGAAGCAG CCATAATAGA AACTAACTGC 
ATTTACCGTC TCATGGATGA CAAACTTGTT CCTGATGACG ACTACTGGGG GAAAATTCCG 
AAGTGCTCCC TTCTGCAACC CAAAGAGGTA CTGGTGTTTG ATTTTGGTAG TGAAGTTTAC 
GTATGGCATG GGAAAGAAGT CACATTAGCA CAACGAAAAA TAGCATTTCA GCTGGCAAAG 
CACTTATGGA ATGGAACCTT TGACTATGAG AACTGTGACA TCAATCCCCT GGATCCTGGA 
GAATGCAATC CGCTTATCCC CAGAAAAGGA CAGGGGCGGC CCGACTGGGC GATATTTGGG 
AGACTTACTG AACACAATGA GACGATTTTG TTCAAAGAGA AGTTTCTGGA TTGGACGGAA 
CTGAAGAGAT CGAATGAGAA GAACCCCGGG GAACTTGCCC AGCACAAGGA AGACCCCAGG 
ACTGATGTCA AGGCATACGA TGTGACACGG ATGGTGTCCA TGCCCCAGAC GACAGCAGGC 
ACCATCCTGG ACGGAGTGAA CGTCGGCCGT GGCTATGGCC TGGTGGAAGG ACACGACAGG 
AGGCAGTTTG AGATCACCAG CGTTTCCGTG GATGTCTGGC ACATCCTGGA ATTCGACTAT 
AGCAGGCTCC CCAAACAAAG CATCGGGCAG TTCCATGAGG GGGATGCCTA TGTGGTCAAG 
TGGAAGTTCA TGGTGAGCAC GGCAGTGGGA AGTCGCCAGA AGGGAGAGCA CTCGGTGAGG 
GCAGCCGGCA AAGAGAAGTG CGTCTACTTC TTCTGGCAAG GCCGGCACTC CACCGTGAGT 
GAGAAGGGCA CGTCGGCGCT GATGACGGTG GAGCTGGACG AGGAAAGGGG GGCCCAGGTC 
CAGGTTCTCC AGGGAAAGGA GCCCCCCTGT TTCCTGCAGT GTTTCCAGGG GGGGATGGTG 
GTGCACTCGG GGAGGCGGGA AGAGGAAGAA GAAAATGTGC AAAGTGAGTG GCGGCTGTAC 
TGCGTGCGTG GAGAGGTGCC CGTGGAAGGG AATTTGCTGG AAGTGGCCTG TCACTGTAGC 
AGCCTGAGGT CCAGAACTTC CATGGTGGTG CTTAACGTCA ACAAGGCCCT CATCTACCTG 
TGGCACGGAT GCAAAGCCCA GGCCCACACG AAGGAGGTCG GAAGGACCGC TGCGAACAAG 
ATCAAGGAAC AATGTCCCCT GGAAGCAGGA CTGCATAGTA GCAGCAAAGT CACAATACAC 
GAGTGTGATG AAGGCTCCGA GCCACTCGGA TTCTGGGATG CCTTAGGAAG GAGAGACAGG 
AAAGCCTACG ATTGCATGCT TCAAGATCCT GGAAGTTTTA ACTTCGCGCC CCGCCTGTTC 
ATCCTCAGCA GCTCCTCTGG GGATTTTGCA GCCACAGAGT TTGTGTACCC TGCCCGAGCC 
CCCTCTGTGG TCAGTTCCAT GCCCTTCCTG CAGGAAGATC TGTACAGCGC GCCCCAGCCA 
GCACTTTTCC TTGTTGACAA TCACCACGAG GTGTACCTCT GGCAAGGCTG GTGGCCCATC 
GAGAACAAGA TCACTGGTTC CGCCCGCATC CGCTGGGCCT CCGACCGGAA GAGTGCGATG 
GAGACTGTGC TCCAGTACTG CAAAGGAAAA AATCTCAAGA AACCAGCCCC CAAGTCTTAC 
CTTATCCACG CTGGTCTGGA GCCCCTGACA TTCACCAATA TGTTTCCCAG CTGGGAGCAC 
AGAGAGGACA TCGCTGAGAT CACAGAGATG GACACGGAAG TTTCCAATCA GATCACCCTC 
GTGGAAGACG TCTTAGCCAA GCTCTGTAAA ACCATTTACC CGCTGGCCGA CCTCCTGGCC 
AGGCCACTCC CGGAGGGGGT CGATCCTCTG AAGCTTGAGA TCTATCTCAC CGACGAAGAC 
TTCGAGTTTG CACTAGACAT GACGAGGGAT GAATACAACG CCCTGCCCGC CTGGAAGCAG 
GTGAACCTGA AGAAAGCAAA AGGCCTGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303907.1
CDS	201..6893
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448139.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448139.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGCGGGTC ACAGAAAGTC GAGAGAGCCA AATGACGATT 
GAGGAGAGGA AGCAGCTCAT CACTGTGAGA GAGGAGGCCT GGAAGACGAG AGGCAGAGGA 
GCGGCCAACG ACTCGACCCA GTTCACTGTG GCTGGCAGGA TGGTGAAGAA AGGTTTGGCG 
TCACCTACTG CCATAACCCC AGTAGCCTCA CCCATTTGCG GTAAAACAAG AGGCACCACA 
CCCGTTTCCA AACCCCTGGA AGATATCGAA GCCAGACCAG ATATGCAGTT AGAATCGGAC 
CTGAAGTTGG ACAGGCTGGA AACCTTTCTA AGAAGGCTGA ATAACAAAGT TGGCGGGATG 
CACGAAACGG TGCTCACTGT CACCGGCAAA TCTGTGAAGG AGGTGATGAA GCCAGATGAT 
GATGAAACCT TTGCCAAATT TTACCGCAGC GTGGATTATA ATATGCCAAG AAGTCCTGTG 
GAGATGGATG AGGACTTCGA TGTCATTTTC GATCCTTATG CACCCAAATT GACGTCTTCC 
GTGGCCGAGC ACAAGCGGGC AGTTAGGCCC AAGCGCCGGG TTCAGGCCTC CAAAAACCCC 
CTGAAAATGC TGGCGGCAAG AGAAGATCTC CTTCAGGAAT ACACTGAGCA GAGATTAAAC 
GTTGCCTTCA TGGAGTCAAA GCGGATGAAA GTAGAAAAGA TGTCTTCCAA CTCCAACTTC 
TCAGAAGTCA CCCTGGCGGG TTTAGCCAGT AAAGAAAACT TCAGCAACGT CAGCCTGCGG 
AGCGTCAACC TGACGGAACA GAACTCTAAC AACAGCGCCG TGCCCTACAA GAGGCTGATG 
CTGTTGCAGA TTAAAGGAAG AAGACATGTG CAGACCAGGC TGGTGGAACC TCGAGCTTCG 
GCGCTCAACA GTGGGGACTG CTTCCTCCTG CTCTCTCCCC ACTGCTGCTT CCTGTGGGTA 
GGAGAGTTTG CAAACGTCAT AGAAAAGGCG AAGGCCTCAG AACTTGCAAC TTTAATTCAG 
ACAAAGAGGG AACTTGGTTG TAGAGCTACT TATATCCAAA CCATTGAAGA AGGAATTAAT 
ACACACACTC ATGCAGCCAA AGACTTCTGG AAGCTTCTGG GTGGCCAAAC CAGTTACCAA 
TCTGCTGGAG ACCCAAAAGA AGATGAACTC TATGAAGCAG CCATAATAGA AACTAACTGC 
ATTTACCGTC TCATGGATGA CAAACTTGTT CCTGATGACG ACTACTGGGG GAAAATTCCG 
AAGTGCTCCC TTCTGCAACC CAAAGAGGTA CTGGTGTTTG ATTTTGGTAG TGAAGTTTAC 
GTATGGCATG GGAAAGAAGT CACATTAGCA CAACGAAAAA TAGCATTTCA GCTGGCAAAG 
CACTTATGGA ATGGAACCTT TGACTATGAG AACTGTGACA TCAATCCCCT GGATCCTGGA 
GAATGCAATC CGCTTATCCC CAGAAAAGGA CAGGGGCGGC CCGACTGGGC GATATTTGGG 
AGACTTACTG AACACAATGA GACGATTTTG TTCAAAGAGA AGTTTCTGGA TTGGACGGAA 
CTGAAGAGAT CGAATGAGAA GAACCCCGGG GAACTTGCCC AGCACAAGGA AGACCCCAGG 
ACTGATGTCA AGGCATACGA TGTGACACGG ATGGTGTCCA TGCCCCAGAC GACAGCAGGC 
ACCATCCTGG ACGGAGTGAA CGTCGGCCGT GGCTATGGCC TGGTGGAAGG ACACGACAGG 
AGGCAGTTTG AGATCACCAG CGTTTCCGTG GATGTCTGGC ACATCCTGGA ATTCGACTAT 
AGCAGGCTCC CCAAACAAAG CATCGGGCAG TTCCATGAGG GGGATGCCTA TGTGGTCAAG 
TGGAAGTTCA TGGTGAGCAC GGCAGTGGGA AGTCGCCAGA AGGGAGAGCA CTCGGTGAGG 
GCAGCCGGCA AAGAGAAGTG CGTCTACTTC TTCTGGCAAG GCCGGCACTC CACCGTGAGT 
GAGAAGGGCA CGTCGGCGCT GATGACGGTG GAGCTGGACG AGGAAAGGGG GGCCCAGGTC 
CAGGTTCTCC AGGGAAAGGA GCCCCCCTGT TTCCTGCAGT GTTTCCAGGG GGGGATGGTG 
GTGCACTCGG GGAGGCGGGA AGAGGAAGAA GAAAATGTGC AAAGTGAGTG GCGGCTGTAC 
TGCGTGCGTG GAGAGGTGCC CGTGGAAGGG AATTTGCTGG AAGTGGCCTG TCACTGTAGC 
AGCCTGAGGT CCAGAACTTC CATGGTGGTG CTTAACGTCA ACAAGGCCCT CATCTACCTG 
TGGCACGGAT GCAAAGCCCA GGCCCACACG AAGGAGGTCG GAAGGACCGC TGCGAACAAG 
ATCAAGGAAC AATGTCCCCT GGAAGCAGGA CTGCATAGTA GCAGCAAAGT CACAATACAC 
GAGTGTGATG AAGGCTCCGA GCCACTCGGA TTCTGGGATG CCTTAGGAAG GAGAGACAGG 
AAAGCCTACG ATTGCATGCT TCAAGATCCT GGAAGTTTTA ACTTCGCGCC CCGCCTGTTC 
ATCCTCAGCA GCTCCTCTGG GGATTTTGCA GCCACAGAGT TTGTGTACCC TGCCCGAGCC 
CCCTCTGTGG TCAGTTCCAT GCCCTTCCTG CAGGAAGATC TGTACAGCGC GCCCCAGCCA 
GCACTTTTCC TTGTTGACAA TCACCACGAG GTGTACCTCT GGCAAGGCTG GTGGCCCATC 
GAGAACAAGA TCACTGGTTC CGCCCGCATC CGCTGGGCCT CCGACCGGAA GAGTGCGATG 
GAGACTGTGC TCCAGTACTG CAAAGGAAAA AATCTCAAGA AACCAGCCCC CAAGTCTTAC 
CTTATCCACG CTGGTCTGGA GCCCCTGACA TTCACCAATA TGTTTCCCAG CTGGGAGCAC 
AGAGAGGACA TCGCTGAGAT CACAGAGATG GACACGGAAG TTTCCAATCA GATCACCCTC 
GTGGAAGACG TCTTAGCCAA GCTCTGTAAA ACCATTTACC CGCTGGCCGA CCTCCTGGCC 
AGGCCACTCC CGGAGGGGGT CGATCCTCTG AAGCTTGAGA TCTATCTCAC CGACGAAGAC 
TTCGAGTTTG CACTAGACAT GACGAGGGAT GAATACAACG CCCTGCCCGC CTGGAAGCAG 
GTGAACCTGA AGAAAGCAAA AGGCCTGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448141.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303909.1 Homo sapiens supervillin (SVIL), transcript variant X4, mRNA.

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CloneID	OHu60823
Clone ID Related Accession (Same CDS sequence)	XM_024448141.1
Accession Version	XM_024448141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACATTG CAGGCCTCTG CTCACCAAAA GGCCTTAGCC 
AAGGACCAGA CAAATGAGGG CAAAGAGCTT GCTGAGCAAG GAGAACCTGA TTCCTCCACT 
CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT AACAAATTGT CCCAGCCAGT CTCAAAAGCG 
ATTTCTACCC GGAACAGAAT AGACACGAGA CAGAGGAGAA TGAACGCTCG CTATCAAACT 
CAGCCAGTCA CACTGGGAGA GGTGGAGCAG GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA 
CCTGCCGTGA ACACATCAGT GTCTACCGTA GCATCCACGG TTGCTCCAAT GTATGCCGGA 
GATCTTCGCA CAAAGCCACC TCTTGACCAC AATGCAAGTG CCACTGACTA TAAGTTTTCT 
TCTTCAATAG AAAATTCGGA CTCTCCAGTT AGAAGCATTC TGAAATCGCA AGCTTGGCAG 
CCTTTGGTAG AGGGTAGCGA GAACAAGGGA ATGTTGAGAG AATATGGAGA GACAGAAAGC 
AAGAGAGCTT TGACAGGTCG AGACAGTGGG ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA 
GAAGCATCCT ACCCCATCCT GAACCGAGCC AGGGAAGGAG ACAGCCATAA GGAATCTAAA 
TATGCTGTTC CCAGAAGAGG AAGCCTGGAA CGGGCGAACC CTCCCATCAC CCACCTCGGG 
GATGAACCGA AGGAATTTTC CATGGCTAAA ATGAATGCAC AAGGAAACTT GGACTTGAGG 
GACAGGCTGC CCTTTGAAGA GAAGGTGGAG GTGGAGAATG TTATGAAAAG GAAGTTTTCA 
CTAAGAGCGG CAGAGTTCGG GGAGCCCACT TCCGAGCAGA CGGGGACAGC TGCTGGGAAA 
ACTATTGCTC AAACCACAGC CCCCGTGTCC TGGAAGCCCC AGGATTCTTC GGAACAGCCA 
CAGGAGAAGC TCTGCAAGAA TCCATGTGCG ATGTTTGCTG CTGGAGAGAT CAAAACGCCG 
ACAGGGGAGG GCCTTCTTGA CTCACCCAGC AAAACCATGT CTATTAAAGA AAGATTGGCA 
CTGTTGAAGA AAAGCGGGGA GGAAGATTGG AGAAACAGAC TCAGCAGGAG GCAGGAGGGC 
GGCAAGGCGC CGGCCAGCAG CCTGCACACC CAGGAAGCAG GGCGGTCCCT CATCAAGAAG 
GAAGAAGGAG GAGTTGCGGA TGATAGTGCC ATTTCTAATC TGCTTTGGGA ACCTGTATAT 
GCTTCTACTT ATTCTCCTGC TATTCCTGCT GCCCATAAAT ACCTGTCTTT TGTGTCGATT 
AATCAGCGGG TCACAGAAAG TCGAGAGAGC CAAATGACGA TTGAGGAGAG GAAGCAGCTC 
ATCACTGTGA GAGAGGAGGC CTGGAAGACG AGAGGCAGAG GAGCGGCCAA CGACTCGACC 
CAGTTCACTG TGGCTGGCAG GATGGTGAAG AAAGGTTTGG CGTCACCTAC TGCCATAACC 
CCAGTAGCCT CACCCATTTG CGGTAAAACA AGAGGCACCA CACCCGTTTC CAAACCCCTG 
GAAGATATCG AAGCCAGACC AGATATGCAG TTAGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGCGGGA TGCACGAAAC GGTGCTCACT 
GTCACCGGCA AATCTGTGAA GGAGGTGATG AAGCCAGATG ATGATGAAAC CTTTGCCAAA 
TTTTACCGCA GCGTGGATTA TAATATGCCA AGAAGTCCTG TGGAGATGGA TGAGGACTTC 
GATGTCATTT TCGATCCTTA TGCACCCAAA TTGACGTCTT CCGTGGCCGA GCACAAGCGG 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC TCCAAAAACC CCCTGAAAAT GCTGGCGGCA 
AGAGAAGATC TCCTTCAGGA ATACACTGAG CAGAGATTAA ACGTTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCTTCC AACTCCAACT TCTCAGAAGT CACCCTGGCG 
GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAACTCTA ACAACAGCGC CGTGCCCTAC AAGAGGCTGA TGCTGTTGCA GATTAAAGGA 
AGAAGACATG TGCAGACCAG GCTGGTGGAA CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCTCC CCACTGCTGC TTCCTGTGGG TAGGAGAGTT TGCAAACGTC 
ATAGAAAAGG CGAAGGCCTC AGAACTTGCA ACTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CTTATATCCA AACCATTGAA GAAGGAATTA ATACACACAC TCATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AATCTGCTGG AGACCCAAAA 
GAAGATGAAC TCTATGAAGC AGCCATAATA GAAACTAACT GCATTTACCG TCTCATGGAT 
GACAAACTTG TTCCTGATGA CGACTACTGG GGGAAAATTC CGAAGTGCTC CCTTCTGCAA 
CCCAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTTT ACGTATGGCA TGGGAAAGAA 
GTCACATTAG CACAACGAAA AATAGCATTT CAGCTGGCAA AGCACTTATG GAATGGAACC 
TTTGACTATG AGAACTGTGA CATCAATCCC CTGGATCCTG GAGAATGCAA TCCGCTTATC 
CCCAGAAAAG GACAGGGGCG GCCCGACTGG GCGATATTTG GGAGACTTAC TGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAGTTTCTG GATTGGACGG AACTGAAGAG ATCGAATGAG 
AAGAACCCCG GGGAACTTGC CCAGCACAAG GAAGACCCCA GGACTGATGT CAAGGCATAC 
GATGTGACAC GGATGGTGTC CATGCCCCAG ACGACAGCAG GCACCATCCT GGACGGAGTG 
AACGTCGGCC GTGGCTATGG CCTGGTGGAA GGACACGACA GGAGGCAGTT TGAGATCACC 
AGCGTTTCCG TGGATGTCTG GCACATCCTG GAATTCGACT ATAGCAGGCT CCCCAAACAA 
AGCATCGGGC AGTTCCATGA GGGGGATGCC TATGTGGTCA AGTGGAAGTT CATGGTGAGC 
ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG CACTCGGTGA GGGCAGCCGG CAAAGAGAAG 
TGCGTCTACT TCTTCTGGCA AGGCCGGCAC TCCACCGTGA GTGAGAAGGG CACGTCGGCG 
CTGATGACGG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG 
GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG GGGGGGATGG TGGTGCACTC GGGGAGGCGG 
GAAGAGGAAG AAGAAAATGT GCAAAGTGAG TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG 
CCCGTGGAAG GGAATTTGCT GGAAGTGGCC TGTCACTGTA GCAGCCTGAG GTCCAGAACT 
TCCATGGTGG TGCTTAACGT CAACAAGGCC CTCATCTACC TGTGGCACGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGAAGGACC GCTGCGAACA AGATCAAGGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTCACAATAC ACGAGTGTGA TGAAGGCTCC 
GAGCCACTCG GATTCTGGGA TGCCTTAGGA AGGAGAGACA GGAAAGCCTA CGATTGCATG 
CTTCAAGATC CTGGAAGTTT TAACTTCGCG CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGGGATTTTG CAGCCACAGA GTTTGTGTAC CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC 
ATGCCCTTCC TGCAGGAAGA TCTGTACAGC GCGCCCCAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACTGGT 
TCCGCCCGCA TCCGCTGGGC CTCCGACCGG AAGAGTGCGA TGGAGACTGT GCTCCAGTAC 
TGCAAAGGAA AAAATCTCAA GAAACCAGCC CCCAAGTCTT ACCTTATCCA CGCTGGTCTG 
GAGCCCCTGA CATTCACCAA TATGTTTCCC AGCTGGGAGC ACAGAGAGGA CATCGCTGAG 
ATCACAGAGA TGGACACGGA AGTTTCCAAT CAGATCACCC TCGTGGAAGA CGTCTTAGCC 
AAGCTCTGTA AAACCATTTA CCCGCTGGCC GACCTCCTGG CCAGGCCACT CCCGGAGGGG 
GTCGATCCTC TGAAGCTTGA GATCTATCTC ACCGACGAAG ACTTCGAGTT TGCACTAGAC 
ATGACGAGGG ATGAATACAA CGCCCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303909.1
CDS	201..6875
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448141.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448141.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACATTG CAGGCCTCTG CTCACCAAAA GGCCTTAGCC 
AAGGACCAGA CAAATGAGGG CAAAGAGCTT GCTGAGCAAG GAGAACCTGA TTCCTCCACT 
CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT AACAAATTGT CCCAGCCAGT CTCAAAAGCG 
ATTTCTACCC GGAACAGAAT AGACACGAGA CAGAGGAGAA TGAACGCTCG CTATCAAACT 
CAGCCAGTCA CACTGGGAGA GGTGGAGCAG GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA 
CCTGCCGTGA ACACATCAGT GTCTACCGTA GCATCCACGG TTGCTCCAAT GTATGCCGGA 
GATCTTCGCA CAAAGCCACC TCTTGACCAC AATGCAAGTG CCACTGACTA TAAGTTTTCT 
TCTTCAATAG AAAATTCGGA CTCTCCAGTT AGAAGCATTC TGAAATCGCA AGCTTGGCAG 
CCTTTGGTAG AGGGTAGCGA GAACAAGGGA ATGTTGAGAG AATATGGAGA GACAGAAAGC 
AAGAGAGCTT TGACAGGTCG AGACAGTGGG ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA 
GAAGCATCCT ACCCCATCCT GAACCGAGCC AGGGAAGGAG ACAGCCATAA GGAATCTAAA 
TATGCTGTTC CCAGAAGAGG AAGCCTGGAA CGGGCGAACC CTCCCATCAC CCACCTCGGG 
GATGAACCGA AGGAATTTTC CATGGCTAAA ATGAATGCAC AAGGAAACTT GGACTTGAGG 
GACAGGCTGC CCTTTGAAGA GAAGGTGGAG GTGGAGAATG TTATGAAAAG GAAGTTTTCA 
CTAAGAGCGG CAGAGTTCGG GGAGCCCACT TCCGAGCAGA CGGGGACAGC TGCTGGGAAA 
ACTATTGCTC AAACCACAGC CCCCGTGTCC TGGAAGCCCC AGGATTCTTC GGAACAGCCA 
CAGGAGAAGC TCTGCAAGAA TCCATGTGCG ATGTTTGCTG CTGGAGAGAT CAAAACGCCG 
ACAGGGGAGG GCCTTCTTGA CTCACCCAGC AAAACCATGT CTATTAAAGA AAGATTGGCA 
CTGTTGAAGA AAAGCGGGGA GGAAGATTGG AGAAACAGAC TCAGCAGGAG GCAGGAGGGC 
GGCAAGGCGC CGGCCAGCAG CCTGCACACC CAGGAAGCAG GGCGGTCCCT CATCAAGAAG 
GAAGAAGGAG GAGTTGCGGA TGATAGTGCC ATTTCTAATC TGCTTTGGGA ACCTGTATAT 
GCTTCTACTT ATTCTCCTGC TATTCCTGCT GCCCATAAAT ACCTGTCTTT TGTGTCGATT 
AATCAGCGGG TCACAGAAAG TCGAGAGAGC CAAATGACGA TTGAGGAGAG GAAGCAGCTC 
ATCACTGTGA GAGAGGAGGC CTGGAAGACG AGAGGCAGAG GAGCGGCCAA CGACTCGACC 
CAGTTCACTG TGGCTGGCAG GATGGTGAAG AAAGGTTTGG CGTCACCTAC TGCCATAACC 
CCAGTAGCCT CACCCATTTG CGGTAAAACA AGAGGCACCA CACCCGTTTC CAAACCCCTG 
GAAGATATCG AAGCCAGACC AGATATGCAG TTAGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGCGGGA TGCACGAAAC GGTGCTCACT 
GTCACCGGCA AATCTGTGAA GGAGGTGATG AAGCCAGATG ATGATGAAAC CTTTGCCAAA 
TTTTACCGCA GCGTGGATTA TAATATGCCA AGAAGTCCTG TGGAGATGGA TGAGGACTTC 
GATGTCATTT TCGATCCTTA TGCACCCAAA TTGACGTCTT CCGTGGCCGA GCACAAGCGG 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC TCCAAAAACC CCCTGAAAAT GCTGGCGGCA 
AGAGAAGATC TCCTTCAGGA ATACACTGAG CAGAGATTAA ACGTTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCTTCC AACTCCAACT TCTCAGAAGT CACCCTGGCG 
GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAACTCTA ACAACAGCGC CGTGCCCTAC AAGAGGCTGA TGCTGTTGCA GATTAAAGGA 
AGAAGACATG TGCAGACCAG GCTGGTGGAA CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCTCC CCACTGCTGC TTCCTGTGGG TAGGAGAGTT TGCAAACGTC 
ATAGAAAAGG CGAAGGCCTC AGAACTTGCA ACTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CTTATATCCA AACCATTGAA GAAGGAATTA ATACACACAC TCATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AATCTGCTGG AGACCCAAAA 
GAAGATGAAC TCTATGAAGC AGCCATAATA GAAACTAACT GCATTTACCG TCTCATGGAT 
GACAAACTTG TTCCTGATGA CGACTACTGG GGGAAAATTC CGAAGTGCTC CCTTCTGCAA 
CCCAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTTT ACGTATGGCA TGGGAAAGAA 
GTCACATTAG CACAACGAAA AATAGCATTT CAGCTGGCAA AGCACTTATG GAATGGAACC 
TTTGACTATG AGAACTGTGA CATCAATCCC CTGGATCCTG GAGAATGCAA TCCGCTTATC 
CCCAGAAAAG GACAGGGGCG GCCCGACTGG GCGATATTTG GGAGACTTAC TGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAGTTTCTG GATTGGACGG AACTGAAGAG ATCGAATGAG 
AAGAACCCCG GGGAACTTGC CCAGCACAAG GAAGACCCCA GGACTGATGT CAAGGCATAC 
GATGTGACAC GGATGGTGTC CATGCCCCAG ACGACAGCAG GCACCATCCT GGACGGAGTG 
AACGTCGGCC GTGGCTATGG CCTGGTGGAA GGACACGACA GGAGGCAGTT TGAGATCACC 
AGCGTTTCCG TGGATGTCTG GCACATCCTG GAATTCGACT ATAGCAGGCT CCCCAAACAA 
AGCATCGGGC AGTTCCATGA GGGGGATGCC TATGTGGTCA AGTGGAAGTT CATGGTGAGC 
ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG CACTCGGTGA GGGCAGCCGG CAAAGAGAAG 
TGCGTCTACT TCTTCTGGCA AGGCCGGCAC TCCACCGTGA GTGAGAAGGG CACGTCGGCG 
CTGATGACGG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG 
GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG GGGGGGATGG TGGTGCACTC GGGGAGGCGG 
GAAGAGGAAG AAGAAAATGT GCAAAGTGAG TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG 
CCCGTGGAAG GGAATTTGCT GGAAGTGGCC TGTCACTGTA GCAGCCTGAG GTCCAGAACT 
TCCATGGTGG TGCTTAACGT CAACAAGGCC CTCATCTACC TGTGGCACGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGAAGGACC GCTGCGAACA AGATCAAGGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTCACAATAC ACGAGTGTGA TGAAGGCTCC 
GAGCCACTCG GATTCTGGGA TGCCTTAGGA AGGAGAGACA GGAAAGCCTA CGATTGCATG 
CTTCAAGATC CTGGAAGTTT TAACTTCGCG CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGGGATTTTG CAGCCACAGA GTTTGTGTAC CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC 
ATGCCCTTCC TGCAGGAAGA TCTGTACAGC GCGCCCCAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACTGGT 
TCCGCCCGCA TCCGCTGGGC CTCCGACCGG AAGAGTGCGA TGGAGACTGT GCTCCAGTAC 
TGCAAAGGAA AAAATCTCAA GAAACCAGCC CCCAAGTCTT ACCTTATCCA CGCTGGTCTG 
GAGCCCCTGA CATTCACCAA TATGTTTCCC AGCTGGGAGC ACAGAGAGGA CATCGCTGAG 
ATCACAGAGA TGGACACGGA AGTTTCCAAT CAGATCACCC TCGTGGAAGA CGTCTTAGCC 
AAGCTCTGTA AAACCATTTA CCCGCTGGCC GACCTCCTGG CCAGGCCACT CCCGGAGGGG 
GTCGATCCTC TGAAGCTTGA GATCTATCTC ACCGACGAAG ACTTCGAGTT TGCACTAGAC 
ATGACGAGGG ATGAATACAA CGCCCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448142.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303910.1 Homo sapiens supervillin (SVIL), transcript variant X5, mRNA.

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CloneID	OHu60824
Clone ID Related Accession (Same CDS sequence)	XM_024448142.1
Accession Version	XM_024448142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTGT GCACTTCACA TTCAGAAACG 
CCAACTGTCG ATGATGAAGA AAAGGTGGAT GAACGAGCCA AGCTGAGCGT CGCCGCCAAG 
AGGTTGCTTT TCAGGGAGAT GGAAAAATCT TTTGATGAAC AAAATGTTCC AAAGCGACGC 
TCAAGAAACA CAGCTGTGGA GCAGAGGCTA CGCCGTCTGC AGGACAGGTC CCTCACCCAG 
CCCATCACCA CTGAAGAGGT GGTCATCGCA GCCACTGAAC CTATCCCCGC TTCGTGTTCT 
GGGGGCACCC ACCCTGTAAT GGCGAGACTT CCTAGCCCCA CTGTAGCTAG GAGCGCTGTG 
CAGCCTGCCA GATTGCAGGC CTCTGCTCAC CAAAAGGCCT TAGCCAAGGA CCAGACAAAT 
GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA CCTGATTCCT CCACTCTAAG CTTGGCCGAA 
AAGTTGGCCT TGTTTAACAA ATTGTCCCAG CCAGTCTCAA AAGCGATTTC TACCCGGAAC 
AGAATAGACA CGAGACAGAG GAGAATGAAC GCTCGCTATC AAACTCAGCC AGTCACACTG 
GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG CTCATTCCTT TCTCACCTGC CGTGAACACA 
TCAGTGTCTA CCGTAGCATC CACGGTTGCT CCAATGTATG CCGGAGATCT TCGCACAAAG 
CCACCTCTTG ACCACAATGC AAGTGCCACT GACTATAAGT TTTCTTCTTC AATAGAAAAT 
TCGGACTCTC CAGTTAGAAG CATTCTGAAA TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT 
AGCGAGAACA AGGGAATGTT GAGAGAATAT GGAGAGACAG AAAGCAAGAG AGCTTTGACA 
GGTCGAGACA GTGGGATGGA GAAGTATGGG TCCTTTGAGG AAGCAGAAGC ATCCTACCCC 
ATCCTGAACC GAGCCAGGGA AGGAGACAGC CATAAGGAAT CTAAATATGC TGTTCCCAGA 
AGAGGAAGCC TGGAACGGGC GAACCCTCCC ATCACCCACC TCGGGGATGA ACCGAAGGAA 
TTTTCCATGG CTAAAATGAA TGCACAAGGA AACTTGGACT TGAGGGACAG GCTGCCCTTT 
GAAGAGAAGG TGGAGGTGGA GAATGTTATG AAAAGGAAGT TTTCACTAAG AGCGGCAGAG 
TTCGGGGAGC CCACTTCCGA GCAGACGGGG ACAGCTGCTG GGAAAACTAT TGCTCAAACC 
ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT TCTTCGGAAC AGCCACAGGA GAAGCTCTGC 
AAGAATCCAT GTGCGATGTT TGCTGCTGGA GAGATCAAAA CGCCGACAGG GGAGGGCCTT 
CTTGACTCAC CCAGCAAAAC CATGTCTATT AAAGAAAGAT TGGCACTGTT GAAGAAAAGC 
GGGGAGGAAG ATTGGAGAAA CAGACTCAGC AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC 
AGCAGCCTGC ACACCCAGGA AGCAGGGCGG TCCCTCATCA AGAAGGAAGA AGGAGGAGTT 
GCGGATGATA GTGCCATTTC TAATCTGCTT TGGGAACCTG TATATGCTTC TACTTATTCT 
CCTGCTATTC CTGCTGCCCA TAAATACCTG TCTTTTGTGT CGATTAATCA GCGGGTCACA 
GAAAGTCGAG AGAGCCAAAT GACGATTGAG GAGAGGAAGC AGCTCATCAC TGTGAGAGAG 
GAGGCCTGGA AGACGAGAGG CAGAGGAGCG GCCAACGACT CGACCCAGTT CACTGTGGCT 
GGCAGGATGG TGAAGAAAGG TTTGGCGTCA CCTACTGCCA TAACCCCAGT AGCCTCACCC 
ATTTGCGGTA AAACAAGAGG CACCACACCC GTTTCCAAAC CCCTGGAAGA TATCGAAGCC 
AGACCAGATA TGCAGTTAGA ATCGGACCTG AAGTTGGACA GGCTGGAAAC CTTTCTAAGA 
AGGCTGAATA ACAAAGTTGG CGGGATGCAC GAAACGGTGC TCACTGTCAC CGGCAAATCT 
GTGAAGGAGG TGATGAAGCC AGATGATGAT GAAACCTTTG CCAAATTTTA CCGCAGCGTG 
GATTATAATA TGCCAAGAAG TCCTGTGGAG ATGGATGAGG ACTTCGATGT CATTTTCGAT 
CCTTATGCAC CCAAATTGAC GTCTTCCGTG GCCGAGCACA AGCGGGCAGT TAGGCCCAAG 
CGCCGGGTTC AGGCCTCCAA AAACCCCCTG AAAATGCTGG CGGCAAGAGA AGATCTCCTT 
CAGGAATACA CTGAGCAGAG ATTAAACGTT GCCTTCATGG AGTCAAAGCG GATGAAAGTA 
GAAAAGATGT CTTCCAACTC CAACTTCTCA GAAGTCACCC TGGCGGGTTT AGCCAGTAAA 
GAAAACTTCA GCAACGTCAG CCTGCGGAGC GTCAACCTGA CGGAACAGAA CTCTAACAAC 
AGCGCCGTGC CCTACAAGAG GCTGATGCTG TTGCAGATTA AAGGAAGAAG ACATGTGCAG 
ACCAGGCTGG TGGAACCTCG AGCTTCGGCG CTCAACAGTG GGGACTGCTT CCTCCTGCTC 
TCTCCCCACT GCTGCTTCCT GTGGGTAGGA GAGTTTGCAA ACGTCATAGA AAAGGCGAAG 
GCCTCAGAAC TTGCAACTTT AATTCAGACA AAGAGGGAAC TTGGTTGTAG AGCTACTTAT 
ATCCAAACCA TTGAAGAAGG AATTAATACA CACACTCATG CAGCCAAAGA CTTCTGGAAG 
CTTCTGGGTG GCCAAACCAG TTACCAATCT GCTGGAGACC CAAAAGAAGA TGAACTCTAT 
GAAGCAGCCA TAATAGAAAC TAACTGCATT TACCGTCTCA TGGATGACAA ACTTGTTCCT 
GATGACGACT ACTGGGGGAA AATTCCGAAG TGCTCCCTTC TGCAACCCAA AGAGGTACTG 
GTGTTTGATT TTGGTAGTGA AGTTTACGTA TGGCATGGGA AAGAAGTCAC ATTAGCACAA 
CGAAAAATAG CATTTCAGCT GGCAAAGCAC TTATGGAATG GAACCTTTGA CTATGAGAAC 
TGTGACATCA ATCCCCTGGA TCCTGGAGAA TGCAATCCGC TTATCCCCAG AAAAGGACAG 
GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA CTTACTGAAC ACAATGAGAC GATTTTGTTC 
AAAGAGAAGT TTCTGGATTG GACGGAACTG AAGAGATCGA ATGAGAAGAA CCCCGGGGAA 
CTTGCCCAGC ACAAGGAAGA CCCCAGGACT GATGTCAAGG CATACGATGT GACACGGATG 
GTGTCCATGC CCCAGACGAC AGCAGGCACC ATCCTGGACG GAGTGAACGT CGGCCGTGGC 
TATGGCCTGG TGGAAGGACA CGACAGGAGG CAGTTTGAGA TCACCAGCGT TTCCGTGGAT 
GTCTGGCACA TCCTGGAATT CGACTATAGC AGGCTCCCCA AACAAAGCAT CGGGCAGTTC 
CATGAGGGGG ATGCCTATGT GGTCAAGTGG AAGTTCATGG TGAGCACGGC AGTGGGAAGT 
CGCCAGAAGG GAGAGCACTC GGTGAGGGCA GCCGGCAAAG AGAAGTGCGT CTACTTCTTC 
TGGCAAGGCC GGCACTCCAC CGTGAGTGAG AAGGGCACGT CGGCGCTGAT GACGGTGGAG 
CTGGACGAGG AAAGGGGGGC CCAGGTCCAG GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC 
CTGCAGTGTT TCCAGGGGGG GATGGTGGTG CACTCGGGGA GGCGGGAAGA GGAAGAAGAA 
AATGTGCAAA GTGAGTGGCG GCTGTACTGC GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT 
TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC CTGAGGTCCA GAACTTCCAT GGTGGTGCTT 
AACGTCAACA AGGCCCTCAT CTACCTGTGG CACGGATGCA AAGCCCAGGC CCACACGAAG 
GAGGTCGGAA GGACCGCTGC GAACAAGATC AAGGAACAAT GTCCCCTGGA AGCAGGACTG 
CATAGTAGCA GCAAAGTCAC AATACACGAG TGTGATGAAG GCTCCGAGCC ACTCGGATTC 
TGGGATGCCT TAGGAAGGAG AGACAGGAAA GCCTACGATT GCATGCTTCA AGATCCTGGA 
AGTTTTAACT TCGCGCCCCG CCTGTTCATC CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC 
ACAGAGTTTG TGTACCCTGC CCGAGCCCCC TCTGTGGTCA GTTCCATGCC CTTCCTGCAG 
GAAGATCTGT ACAGCGCGCC CCAGCCAGCA CTTTTCCTTG TTGACAATCA CCACGAGGTG 
TACCTCTGGC AAGGCTGGTG GCCCATCGAG AACAAGATCA CTGGTTCCGC CCGCATCCGC 
TGGGCCTCCG ACCGGAAGAG TGCGATGGAG ACTGTGCTCC AGTACTGCAA AGGAAAAAAT 
CTCAAGAAAC CAGCCCCCAA GTCTTACCTT ATCCACGCTG GTCTGGAGCC CCTGACATTC 
ACCAATATGT TTCCCAGCTG GGAGCACAGA GAGGACATCG CTGAGATCAC AGAGATGGAC 
ACGGAAGTTT CCAATCAGAT CACCCTCGTG GAAGACGTCT TAGCCAAGCT CTGTAAAACC 
ATTTACCCGC TGGCCGACCT CCTGGCCAGG CCACTCCCGG AGGGGGTCGA TCCTCTGAAG 
CTTGAGATCT ATCTCACCGA CGAAGACTTC GAGTTTGCAC TAGACATGAC GAGGGATGAA 
TACAACGCCC TGCCCGCCTG GAAGCAGGTG AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303910.1
CDS	201..6800
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448142.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448142.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTGT GCACTTCACA TTCAGAAACG 
CCAACTGTCG ATGATGAAGA AAAGGTGGAT GAACGAGCCA AGCTGAGCGT CGCCGCCAAG 
AGGTTGCTTT TCAGGGAGAT GGAAAAATCT TTTGATGAAC AAAATGTTCC AAAGCGACGC 
TCAAGAAACA CAGCTGTGGA GCAGAGGCTA CGCCGTCTGC AGGACAGGTC CCTCACCCAG 
CCCATCACCA CTGAAGAGGT GGTCATCGCA GCCACTGAAC CTATCCCCGC TTCGTGTTCT 
GGGGGCACCC ACCCTGTAAT GGCGAGACTT CCTAGCCCCA CTGTAGCTAG GAGCGCTGTG 
CAGCCTGCCA GATTGCAGGC CTCTGCTCAC CAAAAGGCCT TAGCCAAGGA CCAGACAAAT 
GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA CCTGATTCCT CCACTCTAAG CTTGGCCGAA 
AAGTTGGCCT TGTTTAACAA ATTGTCCCAG CCAGTCTCAA AAGCGATTTC TACCCGGAAC 
AGAATAGACA CGAGACAGAG GAGAATGAAC GCTCGCTATC AAACTCAGCC AGTCACACTG 
GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG CTCATTCCTT TCTCACCTGC CGTGAACACA 
TCAGTGTCTA CCGTAGCATC CACGGTTGCT CCAATGTATG CCGGAGATCT TCGCACAAAG 
CCACCTCTTG ACCACAATGC AAGTGCCACT GACTATAAGT TTTCTTCTTC AATAGAAAAT 
TCGGACTCTC CAGTTAGAAG CATTCTGAAA TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT 
AGCGAGAACA AGGGAATGTT GAGAGAATAT GGAGAGACAG AAAGCAAGAG AGCTTTGACA 
GGTCGAGACA GTGGGATGGA GAAGTATGGG TCCTTTGAGG AAGCAGAAGC ATCCTACCCC 
ATCCTGAACC GAGCCAGGGA AGGAGACAGC CATAAGGAAT CTAAATATGC TGTTCCCAGA 
AGAGGAAGCC TGGAACGGGC GAACCCTCCC ATCACCCACC TCGGGGATGA ACCGAAGGAA 
TTTTCCATGG CTAAAATGAA TGCACAAGGA AACTTGGACT TGAGGGACAG GCTGCCCTTT 
GAAGAGAAGG TGGAGGTGGA GAATGTTATG AAAAGGAAGT TTTCACTAAG AGCGGCAGAG 
TTCGGGGAGC CCACTTCCGA GCAGACGGGG ACAGCTGCTG GGAAAACTAT TGCTCAAACC 
ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT TCTTCGGAAC AGCCACAGGA GAAGCTCTGC 
AAGAATCCAT GTGCGATGTT TGCTGCTGGA GAGATCAAAA CGCCGACAGG GGAGGGCCTT 
CTTGACTCAC CCAGCAAAAC CATGTCTATT AAAGAAAGAT TGGCACTGTT GAAGAAAAGC 
GGGGAGGAAG ATTGGAGAAA CAGACTCAGC AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC 
AGCAGCCTGC ACACCCAGGA AGCAGGGCGG TCCCTCATCA AGAAGGAAGA AGGAGGAGTT 
GCGGATGATA GTGCCATTTC TAATCTGCTT TGGGAACCTG TATATGCTTC TACTTATTCT 
CCTGCTATTC CTGCTGCCCA TAAATACCTG TCTTTTGTGT CGATTAATCA GCGGGTCACA 
GAAAGTCGAG AGAGCCAAAT GACGATTGAG GAGAGGAAGC AGCTCATCAC TGTGAGAGAG 
GAGGCCTGGA AGACGAGAGG CAGAGGAGCG GCCAACGACT CGACCCAGTT CACTGTGGCT 
GGCAGGATGG TGAAGAAAGG TTTGGCGTCA CCTACTGCCA TAACCCCAGT AGCCTCACCC 
ATTTGCGGTA AAACAAGAGG CACCACACCC GTTTCCAAAC CCCTGGAAGA TATCGAAGCC 
AGACCAGATA TGCAGTTAGA ATCGGACCTG AAGTTGGACA GGCTGGAAAC CTTTCTAAGA 
AGGCTGAATA ACAAAGTTGG CGGGATGCAC GAAACGGTGC TCACTGTCAC CGGCAAATCT 
GTGAAGGAGG TGATGAAGCC AGATGATGAT GAAACCTTTG CCAAATTTTA CCGCAGCGTG 
GATTATAATA TGCCAAGAAG TCCTGTGGAG ATGGATGAGG ACTTCGATGT CATTTTCGAT 
CCTTATGCAC CCAAATTGAC GTCTTCCGTG GCCGAGCACA AGCGGGCAGT TAGGCCCAAG 
CGCCGGGTTC AGGCCTCCAA AAACCCCCTG AAAATGCTGG CGGCAAGAGA AGATCTCCTT 
CAGGAATACA CTGAGCAGAG ATTAAACGTT GCCTTCATGG AGTCAAAGCG GATGAAAGTA 
GAAAAGATGT CTTCCAACTC CAACTTCTCA GAAGTCACCC TGGCGGGTTT AGCCAGTAAA 
GAAAACTTCA GCAACGTCAG CCTGCGGAGC GTCAACCTGA CGGAACAGAA CTCTAACAAC 
AGCGCCGTGC CCTACAAGAG GCTGATGCTG TTGCAGATTA AAGGAAGAAG ACATGTGCAG 
ACCAGGCTGG TGGAACCTCG AGCTTCGGCG CTCAACAGTG GGGACTGCTT CCTCCTGCTC 
TCTCCCCACT GCTGCTTCCT GTGGGTAGGA GAGTTTGCAA ACGTCATAGA AAAGGCGAAG 
GCCTCAGAAC TTGCAACTTT AATTCAGACA AAGAGGGAAC TTGGTTGTAG AGCTACTTAT 
ATCCAAACCA TTGAAGAAGG AATTAATACA CACACTCATG CAGCCAAAGA CTTCTGGAAG 
CTTCTGGGTG GCCAAACCAG TTACCAATCT GCTGGAGACC CAAAAGAAGA TGAACTCTAT 
GAAGCAGCCA TAATAGAAAC TAACTGCATT TACCGTCTCA TGGATGACAA ACTTGTTCCT 
GATGACGACT ACTGGGGGAA AATTCCGAAG TGCTCCCTTC TGCAACCCAA AGAGGTACTG 
GTGTTTGATT TTGGTAGTGA AGTTTACGTA TGGCATGGGA AAGAAGTCAC ATTAGCACAA 
CGAAAAATAG CATTTCAGCT GGCAAAGCAC TTATGGAATG GAACCTTTGA CTATGAGAAC 
TGTGACATCA ATCCCCTGGA TCCTGGAGAA TGCAATCCGC TTATCCCCAG AAAAGGACAG 
GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA CTTACTGAAC ACAATGAGAC GATTTTGTTC 
AAAGAGAAGT TTCTGGATTG GACGGAACTG AAGAGATCGA ATGAGAAGAA CCCCGGGGAA 
CTTGCCCAGC ACAAGGAAGA CCCCAGGACT GATGTCAAGG CATACGATGT GACACGGATG 
GTGTCCATGC CCCAGACGAC AGCAGGCACC ATCCTGGACG GAGTGAACGT CGGCCGTGGC 
TATGGCCTGG TGGAAGGACA CGACAGGAGG CAGTTTGAGA TCACCAGCGT TTCCGTGGAT 
GTCTGGCACA TCCTGGAATT CGACTATAGC AGGCTCCCCA AACAAAGCAT CGGGCAGTTC 
CATGAGGGGG ATGCCTATGT GGTCAAGTGG AAGTTCATGG TGAGCACGGC AGTGGGAAGT 
CGCCAGAAGG GAGAGCACTC GGTGAGGGCA GCCGGCAAAG AGAAGTGCGT CTACTTCTTC 
TGGCAAGGCC GGCACTCCAC CGTGAGTGAG AAGGGCACGT CGGCGCTGAT GACGGTGGAG 
CTGGACGAGG AAAGGGGGGC CCAGGTCCAG GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC 
CTGCAGTGTT TCCAGGGGGG GATGGTGGTG CACTCGGGGA GGCGGGAAGA GGAAGAAGAA 
AATGTGCAAA GTGAGTGGCG GCTGTACTGC GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT 
TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC CTGAGGTCCA GAACTTCCAT GGTGGTGCTT 
AACGTCAACA AGGCCCTCAT CTACCTGTGG CACGGATGCA AAGCCCAGGC CCACACGAAG 
GAGGTCGGAA GGACCGCTGC GAACAAGATC AAGGAACAAT GTCCCCTGGA AGCAGGACTG 
CATAGTAGCA GCAAAGTCAC AATACACGAG TGTGATGAAG GCTCCGAGCC ACTCGGATTC 
TGGGATGCCT TAGGAAGGAG AGACAGGAAA GCCTACGATT GCATGCTTCA AGATCCTGGA 
AGTTTTAACT TCGCGCCCCG CCTGTTCATC CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC 
ACAGAGTTTG TGTACCCTGC CCGAGCCCCC TCTGTGGTCA GTTCCATGCC CTTCCTGCAG 
GAAGATCTGT ACAGCGCGCC CCAGCCAGCA CTTTTCCTTG TTGACAATCA CCACGAGGTG 
TACCTCTGGC AAGGCTGGTG GCCCATCGAG AACAAGATCA CTGGTTCCGC CCGCATCCGC 
TGGGCCTCCG ACCGGAAGAG TGCGATGGAG ACTGTGCTCC AGTACTGCAA AGGAAAAAAT 
CTCAAGAAAC CAGCCCCCAA GTCTTACCTT ATCCACGCTG GTCTGGAGCC CCTGACATTC 
ACCAATATGT TTCCCAGCTG GGAGCACAGA GAGGACATCG CTGAGATCAC AGAGATGGAC 
ACGGAAGTTT CCAATCAGAT CACCCTCGTG GAAGACGTCT TAGCCAAGCT CTGTAAAACC 
ATTTACCCGC TGGCCGACCT CCTGGCCAGG CCACTCCCGG AGGGGGTCGA TCCTCTGAAG 
CTTGAGATCT ATCTCACCGA CGAAGACTTC GAGTTTGCAC TAGACATGAC GAGGGATGAA 
TACAACGCCC TGCCCGCCTG GAAGCAGGTG AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_024448143.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303911.1 Homo sapiens supervillin (SVIL), transcript variant X6, mRNA.

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CloneID	OHu60825
Clone ID Related Accession (Same CDS sequence)	XM_024448143.1
Accession Version	XM_024448143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACATTG CAGGCCTCTG CTCACCAAAA GGCCTTAGCC 
AAGGACCAGA CAAATGAGGG CAAAGAGCTT GCTGAGCAAG GAGAACCTGA TTCCTCCACT 
CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT AACAAATTGT CCCAGCCAGT CTCAAAAGCG 
ATTTCTACCC GGAACAGAAT AGACACGAGA CAGAGGAGAA TGAACGCTCG CTATCAAACT 
CAGCCAGTCA CACTGGGAGA GGTGGAGCAG GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA 
CCTGCCGTGA ACACATCAGT GTCTACCGTA GCATCCACGG TTGCTCCAAT GTATGCCGGA 
GATCTTCGCA CAAAGCCACC TCTTGACCAC AATGCAAGTG CCACTGACTA TAAGTTTTCT 
TCTTCAATAG AAAATTCGGA CTCTCCAGTT AGAAGCATTC TGAAATCGCA AGCTTGGCAG 
CCTTTGGTAG AGGGTAGCGA GAACAAGGGA ATGTTGAGAG AATATGGAGA GACAGAAAGC 
AAGAGAGCTT TGACAGGTCG AGACAGTGGG ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA 
GAAGCATCCT ACCCCATCCT GAACCGAGCC AGGGAAGGAG ACAGCCATAA GGAATCTAAA 
TATGCTGTTC CCAGAAGAGG AAGCCTGGAA CGGGCGAACC CTCCCATCAC CCACCTCGGG 
GATGAACCGA AGGAATTTTC CATGGCTAAA ATGAATGCAC AAGGAAACTT GGACTTGAGG 
GACAGGCTGC CCTTTGAAGA GAAGGTGGAG GTGGAGAATG TTATGAAAAG GAAGTTTTCA 
CTAAGAGCGG CAGAGTTCGG GGAGCCCACT TCCGAGCAGA CGGGGACAGC TGCTGGGAAA 
ACTATTGCTC AAACCACAGC CCCCGTGTCC TGGAAGCCCC AGGATTCTTC GGAACAGCCA 
CAGGAGAAGC TCTGCAAGAA TCCATGTGCG ATGTTTGCTG CTGGAGAGAT CAAAACGCCG 
ACAGGGGAGG GCCTTCTTGA CTCACCCAGC AAAACCATGT CTATTAAAGA AAGATTGGCA 
CTGTTGAAGA AAAGCGGGGA GGAAGATTGG AGAAACAGAC TCAGCAGGAG GCAGGAGGGC 
GGCAAGGCGC CGGCCAGCAG CCTGCACACC CAGGAAGCAG GGCGGTCCCT CATCAAGAAG 
CGGGTCACAG AAAGTCGAGA GAGCCAAATG ACGATTGAGG AGAGGAAGCA GCTCATCACT 
GTGAGAGAGG AGGCCTGGAA GACGAGAGGC AGAGGAGCGG CCAACGACTC GACCCAGTTC 
ACTGTGGCTG GCAGGATGGT GAAGAAAGGT TTGGCGTCAC CTACTGCCAT AACCCCAGTA 
GCCTCACCCA TTTGCGGTAA AACAAGAGGC ACCACACCCG TTTCCAAACC CCTGGAAGAT 
ATCGAAGCCA GACCAGATAT GCAGTTAGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGC GGGATGCACG AAACGGTGCT CACTGTCACC 
GGCAAATCTG TGAAGGAGGT GATGAAGCCA GATGATGATG AAACCTTTGC CAAATTTTAC 
CGCAGCGTGG ATTATAATAT GCCAAGAAGT CCTGTGGAGA TGGATGAGGA CTTCGATGTC 
ATTTTCGATC CTTATGCACC CAAATTGACG TCTTCCGTGG CCGAGCACAA GCGGGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCCTCCAAA AACCCCCTGA AAATGCTGGC GGCAAGAGAA 
GATCTCCTTC AGGAATACAC TGAGCAGAGA TTAAACGTTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC TTCCAACTCC AACTTCTCAG AAGTCACCCT GGCGGGTTTA 
GCCAGTAAAG AAAACTTCAG CAACGTCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAC 
TCTAACAACA GCGCCGTGCC CTACAAGAGG CTGATGCTGT TGCAGATTAA AGGAAGAAGA 
CATGTGCAGA CCAGGCTGGT GGAACCTCGA GCTTCGGCGC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CTCCCCACTG CTGCTTCCTG TGGGTAGGAG AGTTTGCAAA CGTCATAGAA 
AAGGCGAAGG CCTCAGAACT TGCAACTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACTTATA TCCAAACCAT TGAAGAAGGA ATTAATACAC ACACTCATGC AGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAATCTG CTGGAGACCC AAAAGAAGAT 
GAACTCTATG AAGCAGCCAT AATAGAAACT AACTGCATTT ACCGTCTCAT GGATGACAAA 
CTTGTTCCTG ATGACGACTA CTGGGGGAAA ATTCCGAAGT GCTCCCTTCT GCAACCCAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTTTACGTAT GGCATGGGAA AGAAGTCACA 
TTAGCACAAC GAAAAATAGC ATTTCAGCTG GCAAAGCACT TATGGAATGG AACCTTTGAC 
TATGAGAACT GTGACATCAA TCCCCTGGAT CCTGGAGAAT GCAATCCGCT TATCCCCAGA 
AAAGGACAGG GGCGGCCCGA CTGGGCGATA TTTGGGAGAC TTACTGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAGTT TCTGGATTGG ACGGAACTGA AGAGATCGAA TGAGAAGAAC 
CCCGGGGAAC TTGCCCAGCA CAAGGAAGAC CCCAGGACTG ATGTCAAGGC ATACGATGTG 
ACACGGATGG TGTCCATGCC CCAGACGACA GCAGGCACCA TCCTGGACGG AGTGAACGTC 
GGCCGTGGCT ATGGCCTGGT GGAAGGACAC GACAGGAGGC AGTTTGAGAT CACCAGCGTT 
TCCGTGGATG TCTGGCACAT CCTGGAATTC GACTATAGCA GGCTCCCCAA ACAAAGCATC 
GGGCAGTTCC ATGAGGGGGA TGCCTATGTG GTCAAGTGGA AGTTCATGGT GAGCACGGCA 
GTGGGAAGTC GCCAGAAGGG AGAGCACTCG GTGAGGGCAG CCGGCAAAGA GAAGTGCGTC 
TACTTCTTCT GGCAAGGCCG GCACTCCACC GTGAGTGAGA AGGGCACGTC GGCGCTGATG 
ACGGTGGAGC TGGACGAGGA AAGGGGGGCC CAGGTCCAGG TTCTCCAGGG AAAGGAGCCC 
CCCTGTTTCC TGCAGTGTTT CCAGGGGGGG ATGGTGGTGC ACTCGGGGAG GCGGGAAGAG 
GAAGAAGAAA ATGTGCAAAG TGAGTGGCGG CTGTACTGCG TGCGTGGAGA GGTGCCCGTG 
GAAGGGAATT TGCTGGAAGT GGCCTGTCAC TGTAGCAGCC TGAGGTCCAG AACTTCCATG 
GTGGTGCTTA ACGTCAACAA GGCCCTCATC TACCTGTGGC ACGGATGCAA AGCCCAGGCC 
CACACGAAGG AGGTCGGAAG GACCGCTGCG AACAAGATCA AGGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTCACA ATACACGAGT GTGATGAAGG CTCCGAGCCA 
CTCGGATTCT GGGATGCCTT AGGAAGGAGA GACAGGAAAG CCTACGATTG CATGCTTCAA 
GATCCTGGAA GTTTTAACTT CGCGCCCCGC CTGTTCATCC TCAGCAGCTC CTCTGGGGAT 
TTTGCAGCCA CAGAGTTTGT GTACCCTGCC CGAGCCCCCT CTGTGGTCAG TTCCATGCCC 
TTCCTGCAGG AAGATCTGTA CAGCGCGCCC CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTGT ACCTCTGGCA AGGCTGGTGG CCCATCGAGA ACAAGATCAC TGGTTCCGCC 
CGCATCCGCT GGGCCTCCGA CCGGAAGAGT GCGATGGAGA CTGTGCTCCA GTACTGCAAA 
GGAAAAAATC TCAAGAAACC AGCCCCCAAG TCTTACCTTA TCCACGCTGG TCTGGAGCCC 
CTGACATTCA CCAATATGTT TCCCAGCTGG GAGCACAGAG AGGACATCGC TGAGATCACA 
GAGATGGACA CGGAAGTTTC CAATCAGATC ACCCTCGTGG AAGACGTCTT AGCCAAGCTC 
TGTAAAACCA TTTACCCGCT GGCCGACCTC CTGGCCAGGC CACTCCCGGA GGGGGTCGAT 
CCTCTGAAGC TTGAGATCTA TCTCACCGAC GAAGACTTCG AGTTTGCACT AGACATGACG 
AGGGATGAAT ACAACGCCCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAAGGC 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303911.1
CDS	201..6749
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_024448143.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448143.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACATTG CAGGCCTCTG CTCACCAAAA GGCCTTAGCC 
AAGGACCAGA CAAATGAGGG CAAAGAGCTT GCTGAGCAAG GAGAACCTGA TTCCTCCACT 
CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT AACAAATTGT CCCAGCCAGT CTCAAAAGCG 
ATTTCTACCC GGAACAGAAT AGACACGAGA CAGAGGAGAA TGAACGCTCG CTATCAAACT 
CAGCCAGTCA CACTGGGAGA GGTGGAGCAG GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA 
CCTGCCGTGA ACACATCAGT GTCTACCGTA GCATCCACGG TTGCTCCAAT GTATGCCGGA 
GATCTTCGCA CAAAGCCACC TCTTGACCAC AATGCAAGTG CCACTGACTA TAAGTTTTCT 
TCTTCAATAG AAAATTCGGA CTCTCCAGTT AGAAGCATTC TGAAATCGCA AGCTTGGCAG 
CCTTTGGTAG AGGGTAGCGA GAACAAGGGA ATGTTGAGAG AATATGGAGA GACAGAAAGC 
AAGAGAGCTT TGACAGGTCG AGACAGTGGG ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA 
GAAGCATCCT ACCCCATCCT GAACCGAGCC AGGGAAGGAG ACAGCCATAA GGAATCTAAA 
TATGCTGTTC CCAGAAGAGG AAGCCTGGAA CGGGCGAACC CTCCCATCAC CCACCTCGGG 
GATGAACCGA AGGAATTTTC CATGGCTAAA ATGAATGCAC AAGGAAACTT GGACTTGAGG 
GACAGGCTGC CCTTTGAAGA GAAGGTGGAG GTGGAGAATG TTATGAAAAG GAAGTTTTCA 
CTAAGAGCGG CAGAGTTCGG GGAGCCCACT TCCGAGCAGA CGGGGACAGC TGCTGGGAAA 
ACTATTGCTC AAACCACAGC CCCCGTGTCC TGGAAGCCCC AGGATTCTTC GGAACAGCCA 
CAGGAGAAGC TCTGCAAGAA TCCATGTGCG ATGTTTGCTG CTGGAGAGAT CAAAACGCCG 
ACAGGGGAGG GCCTTCTTGA CTCACCCAGC AAAACCATGT CTATTAAAGA AAGATTGGCA 
CTGTTGAAGA AAAGCGGGGA GGAAGATTGG AGAAACAGAC TCAGCAGGAG GCAGGAGGGC 
GGCAAGGCGC CGGCCAGCAG CCTGCACACC CAGGAAGCAG GGCGGTCCCT CATCAAGAAG 
CGGGTCACAG AAAGTCGAGA GAGCCAAATG ACGATTGAGG AGAGGAAGCA GCTCATCACT 
GTGAGAGAGG AGGCCTGGAA GACGAGAGGC AGAGGAGCGG CCAACGACTC GACCCAGTTC 
ACTGTGGCTG GCAGGATGGT GAAGAAAGGT TTGGCGTCAC CTACTGCCAT AACCCCAGTA 
GCCTCACCCA TTTGCGGTAA AACAAGAGGC ACCACACCCG TTTCCAAACC CCTGGAAGAT 
ATCGAAGCCA GACCAGATAT GCAGTTAGAA TCGGACCTGA AGTTGGACAG GCTGGAAACC 
TTTCTAAGAA GGCTGAATAA CAAAGTTGGC GGGATGCACG AAACGGTGCT CACTGTCACC 
GGCAAATCTG TGAAGGAGGT GATGAAGCCA GATGATGATG AAACCTTTGC CAAATTTTAC 
CGCAGCGTGG ATTATAATAT GCCAAGAAGT CCTGTGGAGA TGGATGAGGA CTTCGATGTC 
ATTTTCGATC CTTATGCACC CAAATTGACG TCTTCCGTGG CCGAGCACAA GCGGGCAGTT 
AGGCCCAAGC GCCGGGTTCA GGCCTCCAAA AACCCCCTGA AAATGCTGGC GGCAAGAGAA 
GATCTCCTTC AGGAATACAC TGAGCAGAGA TTAAACGTTG CCTTCATGGA GTCAAAGCGG 
ATGAAAGTAG AAAAGATGTC TTCCAACTCC AACTTCTCAG AAGTCACCCT GGCGGGTTTA 
GCCAGTAAAG AAAACTTCAG CAACGTCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAC 
TCTAACAACA GCGCCGTGCC CTACAAGAGG CTGATGCTGT TGCAGATTAA AGGAAGAAGA 
CATGTGCAGA CCAGGCTGGT GGAACCTCGA GCTTCGGCGC TCAACAGTGG GGACTGCTTC 
CTCCTGCTCT CTCCCCACTG CTGCTTCCTG TGGGTAGGAG AGTTTGCAAA CGTCATAGAA 
AAGGCGAAGG CCTCAGAACT TGCAACTTTA ATTCAGACAA AGAGGGAACT TGGTTGTAGA 
GCTACTTATA TCCAAACCAT TGAAGAAGGA ATTAATACAC ACACTCATGC AGCCAAAGAC 
TTCTGGAAGC TTCTGGGTGG CCAAACCAGT TACCAATCTG CTGGAGACCC AAAAGAAGAT 
GAACTCTATG AAGCAGCCAT AATAGAAACT AACTGCATTT ACCGTCTCAT GGATGACAAA 
CTTGTTCCTG ATGACGACTA CTGGGGGAAA ATTCCGAAGT GCTCCCTTCT GCAACCCAAA 
GAGGTACTGG TGTTTGATTT TGGTAGTGAA GTTTACGTAT GGCATGGGAA AGAAGTCACA 
TTAGCACAAC GAAAAATAGC ATTTCAGCTG GCAAAGCACT TATGGAATGG AACCTTTGAC 
TATGAGAACT GTGACATCAA TCCCCTGGAT CCTGGAGAAT GCAATCCGCT TATCCCCAGA 
AAAGGACAGG GGCGGCCCGA CTGGGCGATA TTTGGGAGAC TTACTGAACA CAATGAGACG 
ATTTTGTTCA AAGAGAAGTT TCTGGATTGG ACGGAACTGA AGAGATCGAA TGAGAAGAAC 
CCCGGGGAAC TTGCCCAGCA CAAGGAAGAC CCCAGGACTG ATGTCAAGGC ATACGATGTG 
ACACGGATGG TGTCCATGCC CCAGACGACA GCAGGCACCA TCCTGGACGG AGTGAACGTC 
GGCCGTGGCT ATGGCCTGGT GGAAGGACAC GACAGGAGGC AGTTTGAGAT CACCAGCGTT 
TCCGTGGATG TCTGGCACAT CCTGGAATTC GACTATAGCA GGCTCCCCAA ACAAAGCATC 
GGGCAGTTCC ATGAGGGGGA TGCCTATGTG GTCAAGTGGA AGTTCATGGT GAGCACGGCA 
GTGGGAAGTC GCCAGAAGGG AGAGCACTCG GTGAGGGCAG CCGGCAAAGA GAAGTGCGTC 
TACTTCTTCT GGCAAGGCCG GCACTCCACC GTGAGTGAGA AGGGCACGTC GGCGCTGATG 
ACGGTGGAGC TGGACGAGGA AAGGGGGGCC CAGGTCCAGG TTCTCCAGGG AAAGGAGCCC 
CCCTGTTTCC TGCAGTGTTT CCAGGGGGGG ATGGTGGTGC ACTCGGGGAG GCGGGAAGAG 
GAAGAAGAAA ATGTGCAAAG TGAGTGGCGG CTGTACTGCG TGCGTGGAGA GGTGCCCGTG 
GAAGGGAATT TGCTGGAAGT GGCCTGTCAC TGTAGCAGCC TGAGGTCCAG AACTTCCATG 
GTGGTGCTTA ACGTCAACAA GGCCCTCATC TACCTGTGGC ACGGATGCAA AGCCCAGGCC 
CACACGAAGG AGGTCGGAAG GACCGCTGCG AACAAGATCA AGGAACAATG TCCCCTGGAA 
GCAGGACTGC ATAGTAGCAG CAAAGTCACA ATACACGAGT GTGATGAAGG CTCCGAGCCA 
CTCGGATTCT GGGATGCCTT AGGAAGGAGA GACAGGAAAG CCTACGATTG CATGCTTCAA 
GATCCTGGAA GTTTTAACTT CGCGCCCCGC CTGTTCATCC TCAGCAGCTC CTCTGGGGAT 
TTTGCAGCCA CAGAGTTTGT GTACCCTGCC CGAGCCCCCT CTGTGGTCAG TTCCATGCCC 
TTCCTGCAGG AAGATCTGTA CAGCGCGCCC CAGCCAGCAC TTTTCCTTGT TGACAATCAC 
CACGAGGTGT ACCTCTGGCA AGGCTGGTGG CCCATCGAGA ACAAGATCAC TGGTTCCGCC 
CGCATCCGCT GGGCCTCCGA CCGGAAGAGT GCGATGGAGA CTGTGCTCCA GTACTGCAAA 
GGAAAAAATC TCAAGAAACC AGCCCCCAAG TCTTACCTTA TCCACGCTGG TCTGGAGCCC 
CTGACATTCA CCAATATGTT TCCCAGCTGG GAGCACAGAG AGGACATCGC TGAGATCACA 
GAGATGGACA CGGAAGTTTC CAATCAGATC ACCCTCGTGG AAGACGTCTT AGCCAAGCTC 
TGTAAAACCA TTTACCCGCT GGCCGACCTC CTGGCCAGGC CACTCCCGGA GGGGGTCGAT 
CCTCTGAAGC TTGAGATCTA TCTCACCGAC GAAGACTTCG AGTTTGCACT AGACATGACG 
AGGGATGAAT ACAACGCCCT GCCCGCCTGG AAGCAGGTGA ACCTGAAGAA AGCAAAAGGC 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_017016575.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872064.1 Homo sapiens supervillin (SVIL), transcript variant X1, mRNA.

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CloneID	OHu91597
Clone ID Related Accession (Same CDS sequence)	XM_017016575.1
Accession Version	XM_017016575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CTGAACCTAT CCCCGCTTCG 
TGTTCTGGGG GCACCCACCC TGTAATGGCG AGACTTCCTA GCCCCACTGT AGCTAGGAGC 
GCTGTGCAGC CTGCCAGATT GCAGGCCTCT GCTCACCAAA AGGCCTTAGC CAAGGACCAG 
ACAAATGAGG GCAAAGAGCT TGCTGAGCAA GGAGAACCTG ATTCCTCCAC TCTAAGCTTG 
GCCGAAAAGT TGGCCTTGTT TAACAAATTG TCCCAGCCAG TCTCAAAAGC GATTTCTACC 
CGGAACAGAA TAGACACGAG ACAGAGGAGA ATGAACGCTC GCTATCAAAC TCAGCCAGTC 
ACACTGGGAG AGGTGGAGCA GGTGCAGAGT GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG 
AACACATCAG TGTCTACCGT AGCATCCACG GTTGCTCCAA TGTATGCCGG AGATCTTCGC 
ACAAAGCCAC CTCTTGACCA CAATGCAAGT GCCACTGACT ATAAGTTTTC TTCTTCAATA 
GAAAATTCGG ACTCTCCAGT TAGAAGCATT CTGAAATCGC AAGCTTGGCA GCCTTTGGTA 
GAGGGTAGCG AGAACAAGGG AATGTTGAGA GAATATGGAG AGACAGAAAG CAAGAGAGCT 
TTGACAGGTC GAGACAGTGG GATGGAGAAG TATGGGTCCT TTGAGGAAGC AGAAGCATCC 
TACCCCATCC TGAACCGAGC CAGGGAAGGA GACAGCCATA AGGAATCTAA ATATGCTGTT 
CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC CCTCCCATCA CCCACCTCGG GGATGAACCG 
AAGGAATTTT CCATGGCTAA AATGAATGCA CAAGGAAACT TGGACTTGAG GGACAGGCTG 
CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT GTTATGAAAA GGAAGTTTTC ACTAAGAGCG 
GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG ACGGGGACAG CTGCTGGGAA AACTATTGCT 
CAAACCACAG CCCCCGTGTC CTGGAAGCCC CAGGATTCTT CGGAACAGCC ACAGGAGAAG 
CTCTGCAAGA ATCCATGTGC GATGTTTGCT GCTGGAGAGA TCAAAACGCC GACAGGGGAG 
GGCCTTCTTG ACTCACCCAG CAAAACCATG TCTATTAAAG AAAGATTGGC ACTGTTGAAG 
AAAAGCGGGG AGGAAGATTG GAGAAACAGA CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG 
CCGGCCAGCA GCCTGCACAC CCAGGAAGCA GGGCGGTCCC TCATCAAGAA GGAAGAAGGA 
GGAGTTGCGG ATGATAGTGC CATTTCTAAT CTGCTTTGGG AACCTGTATA TGCTTCTACT 
TATTCTCCTG CTATTCCTGC TGCCCATAAA TACCTGTCTT TTGTGTCGAT TAATCAGCGG 
GTCACAGAAA GTCGAGAGAG CCAAATGACG ATTGAGGAGA GGAAGCAGCT CATCACTGTG 
AGAGAGGAGG CCTGGAAGAC GAGAGGCAGA GGAGCGGCCA ACGACTCGAC CCAGTTCACT 
GTGGCTGGCA GGATGGTGAA GAAAGGTTTG GCGTCACCTA CTGCCATAAC CCCAGTAGCC 
TCACCCATTT GCGGTAAAAC AAGAGGCACC ACACCCGTTT CCAAACCCCT GGAAGATATC 
GAAGCCAGAC CAGATATGCA GTTAGAATCG GACCTGAAGT TGGACAGGCT GGAAACCTTT 
CTAAGAAGGC TGAATAACAA AGTTGGCGGG ATGCACGAAA CGGTGCTCAC TGTCACCGGC 
AAATCTGTGA AGGAGGTGAT GAAGCCAGAT GATGATGAAA CCTTTGCCAA ATTTTACCGC 
AGCGTGGATT ATAATATGCC AAGAAGTCCT GTGGAGATGG ATGAGGACTT CGATGTCATT 
TTCGATCCTT ATGCACCCAA ATTGACGTCT TCCGTGGCCG AGCACAAGCG GGCAGTTAGG 
CCCAAGCGCC GGGTTCAGGC CTCCAAAAAC CCCCTGAAAA TGCTGGCGGC AAGAGAAGAT 
CTCCTTCAGG AATACACTGA GCAGAGATTA AACGTTGCCT TCATGGAGTC AAAGCGGATG 
AAAGTAGAAA AGATGTCTTC CAACTCCAAC TTCTCAGAAG TCACCCTGGC GGGTTTAGCC 
AGTAAAGAAA ACTTCAGCAA CGTCAGCCTG CGGAGCGTCA ACCTGACGGA ACAGAACTCT 
AACAACAGCG CCGTGCCCTA CAAGAGGCTG ATGCTGTTGC AGATTAAAGG AAGAAGACAT 
GTGCAGACCA GGCTGGTGGA ACCTCGAGCT TCGGCGCTCA ACAGTGGGGA CTGCTTCCTC 
CTGCTCTCTC CCCACTGCTG CTTCCTGTGG GTAGGAGAGT TTGCAAACGT CATAGAAAAG 
GCGAAGGCCT CAGAACTTGC AACTTTAATT CAGACAAAGA GGGAACTTGG TTGTAGAGCT 
ACTTATATCC AAACCATTGA AGAAGGAATT AATACACACA CTCATGCAGC CAAAGACTTC 
TGGAAGCTTC TGGGTGGCCA AACCAGTTAC CAATCTGCTG GAGACCCAAA AGAAGATGAA 
CTCTATGAAG CAGCCATAAT AGAAACTAAC TGCATTTACC GTCTCATGGA TGACAAACTT 
GTTCCTGATG ACGACTACTG GGGGAAAATT CCGAAGTGCT CCCTTCTGCA ACCCAAAGAG 
GTACTGGTGT TTGATTTTGG TAGTGAAGTT TACGTATGGC ATGGGAAAGA AGTCACATTA 
GCACAACGAA AAATAGCATT TCAGCTGGCA AAGCACTTAT GGAATGGAAC CTTTGACTAT 
GAGAACTGTG ACATCAATCC CCTGGATCCT GGAGAATGCA ATCCGCTTAT CCCCAGAAAA 
GGACAGGGGC GGCCCGACTG GGCGATATTT GGGAGACTTA CTGAACACAA TGAGACGATT 
TTGTTCAAAG AGAAGTTTCT GGATTGGACG GAACTGAAGA GATCGAATGA GAAGAACCCC 
GGGGAACTTG CCCAGCACAA GGAAGACCCC AGGACTGATG TCAAGGCATA CGATGTGACA 
CGGATGGTGT CCATGCCCCA GACGACAGCA GGCACCATCC TGGACGGAGT GAACGTCGGC 
CGTGGCTATG GCCTGGTGGA AGGACACGAC AGGAGGCAGT TTGAGATCAC CAGCGTTTCC 
GTGGATGTCT GGCACATCCT GGAATTCGAC TATAGCAGGC TCCCCAAACA AAGCATCGGG 
CAGTTCCATG AGGGGGATGC CTATGTGGTC AAGTGGAAGT TCATGGTGAG CACGGCAGTG 
GGAAGTCGCC AGAAGGGAGA GCACTCGGTG AGGGCAGCCG GCAAAGAGAA GTGCGTCTAC 
TTCTTCTGGC AAGGCCGGCA CTCCACCGTG AGTGAGAAGG GCACGTCGGC GCTGATGACG 
GTGGAGCTGG ACGAGGAAAG GGGGGCCCAG GTCCAGGTTC TCCAGGGAAA GGAGCCCCCC 
TGTTTCCTGC AGTGTTTCCA GGGGGGGATG GTGGTGCACT CGGGGAGGCG GGAAGAGGAA 
GAAGAAAATG TGCAAAGTGA GTGGCGGCTG TACTGCGTGC GTGGAGAGGT GCCCGTGGAA 
GGGAATTTGC TGGAAGTGGC CTGTCACTGT AGCAGCCTGA GGTCCAGAAC TTCCATGGTG 
GTGCTTAACG TCAACAAGGC CCTCATCTAC CTGTGGCACG GATGCAAAGC CCAGGCCCAC 
ACGAAGGAGG TCGGAAGGAC CGCTGCGAAC AAGATCAAGG AACAATGTCC CCTGGAAGCA 
GGACTGCATA GTAGCAGCAA AGTCACAATA CACGAGTGTG ATGAAGGCTC CGAGCCACTC 
GGATTCTGGG ATGCCTTAGG AAGGAGAGAC AGGAAAGCCT ACGATTGCAT GCTTCAAGAT 
CCTGGAAGTT TTAACTTCGC GCCCCGCCTG TTCATCCTCA GCAGCTCCTC TGGGGATTTT 
GCAGCCACAG AGTTTGTGTA CCCTGCCCGA GCCCCCTCTG TGGTCAGTTC CATGCCCTTC 
CTGCAGGAAG ATCTGTACAG CGCGCCCCAG CCAGCACTTT TCCTTGTTGA CAATCACCAC 
GAGGTGTACC TCTGGCAAGG CTGGTGGCCC ATCGAGAACA AGATCACTGG TTCCGCCCGC 
ATCCGCTGGG CCTCCGACCG GAAGAGTGCG ATGGAGACTG TGCTCCAGTA CTGCAAAGGA 
AAAAATCTCA AGAAACCAGC CCCCAAGTCT TACCTTATCC ACGCTGGTCT GGAGCCCCTG 
ACATTCACCA ATATGTTTCC CAGCTGGGAG CACAGAGAGG ACATCGCTGA GATCACAGAG 
ATGGACACGG AAGTTTCCAA TCAGATCACC CTCGTGGAAG ACGTCTTAGC CAAGCTCTGT 
AAAACCATTT ACCCGCTGGC CGACCTCCTG GCCAGGCCAC TCCCGGAGGG GGTCGATCCT 
CTGAAGCTTG AGATCTATCT CACCGACGAA GACTTCGAGT TTGCACTAGA CATGACGAGG 
GATGAATACA ACGCCCTGCC CGCCTGGAAG CAGGTGAACC TGAAGAAAGC AAAAGGCCTG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872064.1
CDS	1241..8266
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_017016575.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016575.1

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CTGAACCTAT CCCCGCTTCG 
TGTTCTGGGG GCACCCACCC TGTAATGGCG AGACTTCCTA GCCCCACTGT AGCTAGGAGC 
GCTGTGCAGC CTGCCAGATT GCAGGCCTCT GCTCACCAAA AGGCCTTAGC CAAGGACCAG 
ACAAATGAGG GCAAAGAGCT TGCTGAGCAA GGAGAACCTG ATTCCTCCAC TCTAAGCTTG 
GCCGAAAAGT TGGCCTTGTT TAACAAATTG TCCCAGCCAG TCTCAAAAGC GATTTCTACC 
CGGAACAGAA TAGACACGAG ACAGAGGAGA ATGAACGCTC GCTATCAAAC TCAGCCAGTC 
ACACTGGGAG AGGTGGAGCA GGTGCAGAGT GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG 
AACACATCAG TGTCTACCGT AGCATCCACG GTTGCTCCAA TGTATGCCGG AGATCTTCGC 
ACAAAGCCAC CTCTTGACCA CAATGCAAGT GCCACTGACT ATAAGTTTTC TTCTTCAATA 
GAAAATTCGG ACTCTCCAGT TAGAAGCATT CTGAAATCGC AAGCTTGGCA GCCTTTGGTA 
GAGGGTAGCG AGAACAAGGG AATGTTGAGA GAATATGGAG AGACAGAAAG CAAGAGAGCT 
TTGACAGGTC GAGACAGTGG GATGGAGAAG TATGGGTCCT TTGAGGAAGC AGAAGCATCC 
TACCCCATCC TGAACCGAGC CAGGGAAGGA GACAGCCATA AGGAATCTAA ATATGCTGTT 
CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC CCTCCCATCA CCCACCTCGG GGATGAACCG 
AAGGAATTTT CCATGGCTAA AATGAATGCA CAAGGAAACT TGGACTTGAG GGACAGGCTG 
CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT GTTATGAAAA GGAAGTTTTC ACTAAGAGCG 
GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG ACGGGGACAG CTGCTGGGAA AACTATTGCT 
CAAACCACAG CCCCCGTGTC CTGGAAGCCC CAGGATTCTT CGGAACAGCC ACAGGAGAAG 
CTCTGCAAGA ATCCATGTGC GATGTTTGCT GCTGGAGAGA TCAAAACGCC GACAGGGGAG 
GGCCTTCTTG ACTCACCCAG CAAAACCATG TCTATTAAAG AAAGATTGGC ACTGTTGAAG 
AAAAGCGGGG AGGAAGATTG GAGAAACAGA CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG 
CCGGCCAGCA GCCTGCACAC CCAGGAAGCA GGGCGGTCCC TCATCAAGAA GGAAGAAGGA 
GGAGTTGCGG ATGATAGTGC CATTTCTAAT CTGCTTTGGG AACCTGTATA TGCTTCTACT 
TATTCTCCTG CTATTCCTGC TGCCCATAAA TACCTGTCTT TTGTGTCGAT TAATCAGCGG 
GTCACAGAAA GTCGAGAGAG CCAAATGACG ATTGAGGAGA GGAAGCAGCT CATCACTGTG 
AGAGAGGAGG CCTGGAAGAC GAGAGGCAGA GGAGCGGCCA ACGACTCGAC CCAGTTCACT 
GTGGCTGGCA GGATGGTGAA GAAAGGTTTG GCGTCACCTA CTGCCATAAC CCCAGTAGCC 
TCACCCATTT GCGGTAAAAC AAGAGGCACC ACACCCGTTT CCAAACCCCT GGAAGATATC 
GAAGCCAGAC CAGATATGCA GTTAGAATCG GACCTGAAGT TGGACAGGCT GGAAACCTTT 
CTAAGAAGGC TGAATAACAA AGTTGGCGGG ATGCACGAAA CGGTGCTCAC TGTCACCGGC 
AAATCTGTGA AGGAGGTGAT GAAGCCAGAT GATGATGAAA CCTTTGCCAA ATTTTACCGC 
AGCGTGGATT ATAATATGCC AAGAAGTCCT GTGGAGATGG ATGAGGACTT CGATGTCATT 
TTCGATCCTT ATGCACCCAA ATTGACGTCT TCCGTGGCCG AGCACAAGCG GGCAGTTAGG 
CCCAAGCGCC GGGTTCAGGC CTCCAAAAAC CCCCTGAAAA TGCTGGCGGC AAGAGAAGAT 
CTCCTTCAGG AATACACTGA GCAGAGATTA AACGTTGCCT TCATGGAGTC AAAGCGGATG 
AAAGTAGAAA AGATGTCTTC CAACTCCAAC TTCTCAGAAG TCACCCTGGC GGGTTTAGCC 
AGTAAAGAAA ACTTCAGCAA CGTCAGCCTG CGGAGCGTCA ACCTGACGGA ACAGAACTCT 
AACAACAGCG CCGTGCCCTA CAAGAGGCTG ATGCTGTTGC AGATTAAAGG AAGAAGACAT 
GTGCAGACCA GGCTGGTGGA ACCTCGAGCT TCGGCGCTCA ACAGTGGGGA CTGCTTCCTC 
CTGCTCTCTC CCCACTGCTG CTTCCTGTGG GTAGGAGAGT TTGCAAACGT CATAGAAAAG 
GCGAAGGCCT CAGAACTTGC AACTTTAATT CAGACAAAGA GGGAACTTGG TTGTAGAGCT 
ACTTATATCC AAACCATTGA AGAAGGAATT AATACACACA CTCATGCAGC CAAAGACTTC 
TGGAAGCTTC TGGGTGGCCA AACCAGTTAC CAATCTGCTG GAGACCCAAA AGAAGATGAA 
CTCTATGAAG CAGCCATAAT AGAAACTAAC TGCATTTACC GTCTCATGGA TGACAAACTT 
GTTCCTGATG ACGACTACTG GGGGAAAATT CCGAAGTGCT CCCTTCTGCA ACCCAAAGAG 
GTACTGGTGT TTGATTTTGG TAGTGAAGTT TACGTATGGC ATGGGAAAGA AGTCACATTA 
GCACAACGAA AAATAGCATT TCAGCTGGCA AAGCACTTAT GGAATGGAAC CTTTGACTAT 
GAGAACTGTG ACATCAATCC CCTGGATCCT GGAGAATGCA ATCCGCTTAT CCCCAGAAAA 
GGACAGGGGC GGCCCGACTG GGCGATATTT GGGAGACTTA CTGAACACAA TGAGACGATT 
TTGTTCAAAG AGAAGTTTCT GGATTGGACG GAACTGAAGA GATCGAATGA GAAGAACCCC 
GGGGAACTTG CCCAGCACAA GGAAGACCCC AGGACTGATG TCAAGGCATA CGATGTGACA 
CGGATGGTGT CCATGCCCCA GACGACAGCA GGCACCATCC TGGACGGAGT GAACGTCGGC 
CGTGGCTATG GCCTGGTGGA AGGACACGAC AGGAGGCAGT TTGAGATCAC CAGCGTTTCC 
GTGGATGTCT GGCACATCCT GGAATTCGAC TATAGCAGGC TCCCCAAACA AAGCATCGGG 
CAGTTCCATG AGGGGGATGC CTATGTGGTC AAGTGGAAGT TCATGGTGAG CACGGCAGTG 
GGAAGTCGCC AGAAGGGAGA GCACTCGGTG AGGGCAGCCG GCAAAGAGAA GTGCGTCTAC 
TTCTTCTGGC AAGGCCGGCA CTCCACCGTG AGTGAGAAGG GCACGTCGGC GCTGATGACG 
GTGGAGCTGG ACGAGGAAAG GGGGGCCCAG GTCCAGGTTC TCCAGGGAAA GGAGCCCCCC 
TGTTTCCTGC AGTGTTTCCA GGGGGGGATG GTGGTGCACT CGGGGAGGCG GGAAGAGGAA 
GAAGAAAATG TGCAAAGTGA GTGGCGGCTG TACTGCGTGC GTGGAGAGGT GCCCGTGGAA 
GGGAATTTGC TGGAAGTGGC CTGTCACTGT AGCAGCCTGA GGTCCAGAAC TTCCATGGTG 
GTGCTTAACG TCAACAAGGC CCTCATCTAC CTGTGGCACG GATGCAAAGC CCAGGCCCAC 
ACGAAGGAGG TCGGAAGGAC CGCTGCGAAC AAGATCAAGG AACAATGTCC CCTGGAAGCA 
GGACTGCATA GTAGCAGCAA AGTCACAATA CACGAGTGTG ATGAAGGCTC CGAGCCACTC 
GGATTCTGGG ATGCCTTAGG AAGGAGAGAC AGGAAAGCCT ACGATTGCAT GCTTCAAGAT 
CCTGGAAGTT TTAACTTCGC GCCCCGCCTG TTCATCCTCA GCAGCTCCTC TGGGGATTTT 
GCAGCCACAG AGTTTGTGTA CCCTGCCCGA GCCCCCTCTG TGGTCAGTTC CATGCCCTTC 
CTGCAGGAAG ATCTGTACAG CGCGCCCCAG CCAGCACTTT TCCTTGTTGA CAATCACCAC 
GAGGTGTACC TCTGGCAAGG CTGGTGGCCC ATCGAGAACA AGATCACTGG TTCCGCCCGC 
ATCCGCTGGG CCTCCGACCG GAAGAGTGCG ATGGAGACTG TGCTCCAGTA CTGCAAAGGA 
AAAAATCTCA AGAAACCAGC CCCCAAGTCT TACCTTATCC ACGCTGGTCT GGAGCCCCTG 
ACATTCACCA ATATGTTTCC CAGCTGGGAG CACAGAGAGG ACATCGCTGA GATCACAGAG 
ATGGACACGG AAGTTTCCAA TCAGATCACC CTCGTGGAAG ACGTCTTAGC CAAGCTCTGT 
AAAACCATTT ACCCGCTGGC CGACCTCCTG GCCAGGCCAC TCCCGGAGGG GGTCGATCCT 
CTGAAGCTTG AGATCTATCT CACCGACGAA GACTTCGAGT TTGCACTAGA CATGACGAGG 
GATGAATACA ACGCCCTGCC CGCCTGGAAG CAGGTGAACC TGAAGAAAGC AAAAGGCCTG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519630.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517932.2 Homo sapiens supervillin (SVIL), transcript variant X2, mRNA.

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CloneID	OHu91598
Clone ID Related Accession (Same CDS sequence)	XM_011519630.2
Accession Version	XM_011519630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CTGAACCTAT CCCCGCTTCG 
TGTTCTGGGG GCACCCACCC TGTAATGGCG AGACTTCCTA GCCCCACTGT AGCTAGGAGC 
GCTGTGCAGC CTGCCAGATT GCAGGCCTCT GCTCACCAAA AGGCCTTAGC CAAGGACCAG 
ACAAATGAGG GCAAAGAGCT TGCTGAGCAA GGAGAACCTG ATTCCTCCAC TCTAAGCTTG 
GCCGAAAAGT TGGCCTTGTT TAACAAATTG TCCCAGCCAG TCTCAAAAGC GATTTCTACC 
CGGAACAGAA TAGACACGAG ACAGAGGAGA ATGAACGCTC GCTATCAAAC TCAGCCAGTC 
ACACTGGGAG AGGTGGAGCA GGTGCAGAGT GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG 
AACACATCAG TGTCTACCGT AGCATCCACG GTTGCTCCAA TGTATGCCGG AGATCTTCGC 
ACAAAGCCAC CTCTTGACCA CAATGCAAGT GCCACTGACT ATAAGTTTTC TTCTTCAATA 
GAAAATTCGG ACTCTCCAGT TAGAAGCATT CTGAAATCGC AAGCTTGGCA GCCTTTGGTA 
GAGGGTAGCG AGAACAAGGG AATGTTGAGA GAATATGGAG AGACAGAAAG CAAGAGAGCT 
TTGACAGGTC GAGACAGTGG GATGGAGAAG TATGGGTCCT TTGAGGAAGC AGAAGCATCC 
TACCCCATCC TGAACCGAGC CAGGGAAGGA GACAGCCATA AGGAATCTAA ATATGCTGTT 
CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC CCTCCCATCA CCCACCTCGG GGATGAACCG 
AAGGAATTTT CCATGGCTAA AATGAATGCA CAAGGAAACT TGGACTTGAG GGACAGGCTG 
CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT GTTATGAAAA GGAAGTTTTC ACTAAGAGCG 
GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG ACGGGGACAG CTGCTGGGAA AACTATTGCT 
CAAACCACAG CCCCCGTGTC CTGGAAGCCC CAGGATTCTT CGGAACAGCC ACAGGAGAAG 
CTCTGCAAGA ATCCATGTGC GATGTTTGCT GCTGGAGAGA TCAAAACGCC GACAGGGGAG 
GGCCTTCTTG ACTCACCCAG CAAAACCATG TCTATTAAAG AAAGATTGGC ACTGTTGAAG 
AAAAGCGGGG AGGAAGATTG GAGAAACAGA CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG 
CCGGCCAGCA GCCTGCACAC CCAGGAAGCA GGGCGGTCCC TCATCAAGAA GGAAGAAGGA 
GGAGTTGCGG ATGATAGTGC CATTTCTAAT CTGCTTTGGC GGGTCACAGA AAGTCGAGAG 
AGCCAAATGA CGATTGAGGA GAGGAAGCAG CTCATCACTG TGAGAGAGGA GGCCTGGAAG 
ACGAGAGGCA GAGGAGCGGC CAACGACTCG ACCCAGTTCA CTGTGGCTGG CAGGATGGTG 
AAGAAAGGTT TGGCGTCACC TACTGCCATA ACCCCAGTAG CCTCACCCAT TTGCGGTAAA 
ACAAGAGGCA CCACACCCGT TTCCAAACCC CTGGAAGATA TCGAAGCCAG ACCAGATATG 
CAGTTAGAAT CGGACCTGAA GTTGGACAGG CTGGAAACCT TTCTAAGAAG GCTGAATAAC 
AAAGTTGGCG GGATGCACGA AACGGTGCTC ACTGTCACCG GCAAATCTGT GAAGGAGGTG 
ATGAAGCCAG ATGATGATGA AACCTTTGCC AAATTTTACC GCAGCGTGGA TTATAATATG 
CCAAGAAGTC CTGTGGAGAT GGATGAGGAC TTCGATGTCA TTTTCGATCC TTATGCACCC 
AAATTGACGT CTTCCGTGGC CGAGCACAAG CGGGCAGTTA GGCCCAAGCG CCGGGTTCAG 
GCCTCCAAAA ACCCCCTGAA AATGCTGGCG GCAAGAGAAG ATCTCCTTCA GGAATACACT 
GAGCAGAGAT TAAACGTTGC CTTCATGGAG TCAAAGCGGA TGAAAGTAGA AAAGATGTCT 
TCCAACTCCA ACTTCTCAGA AGTCACCCTG GCGGGTTTAG CCAGTAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAACT CTAACAACAG CGCCGTGCCC 
TACAAGAGGC TGATGCTGTT GCAGATTAAA GGAAGAAGAC ATGTGCAGAC CAGGCTGGTG 
GAACCTCGAG CTTCGGCGCT CAACAGTGGG GACTGCTTCC TCCTGCTCTC TCCCCACTGC 
TGCTTCCTGT GGGTAGGAGA GTTTGCAAAC GTCATAGAAA AGGCGAAGGC CTCAGAACTT 
GCAACTTTAA TTCAGACAAA GAGGGAACTT GGTTGTAGAG CTACTTATAT CCAAACCATT 
GAAGAAGGAA TTAATACACA CACTCATGCA GCCAAAGACT TCTGGAAGCT TCTGGGTGGC 
CAAACCAGTT ACCAATCTGC TGGAGACCCA AAAGAAGATG AACTCTATGA AGCAGCCATA 
ATAGAAACTA ACTGCATTTA CCGTCTCATG GATGACAAAC TTGTTCCTGA TGACGACTAC 
TGGGGGAAAA TTCCGAAGTG CTCCCTTCTG CAACCCAAAG AGGTACTGGT GTTTGATTTT 
GGTAGTGAAG TTTACGTATG GCATGGGAAA GAAGTCACAT TAGCACAACG AAAAATAGCA 
TTTCAGCTGG CAAAGCACTT ATGGAATGGA ACCTTTGACT ATGAGAACTG TGACATCAAT 
CCCCTGGATC CTGGAGAATG CAATCCGCTT ATCCCCAGAA AAGGACAGGG GCGGCCCGAC 
TGGGCGATAT TTGGGAGACT TACTGAACAC AATGAGACGA TTTTGTTCAA AGAGAAGTTT 
CTGGATTGGA CGGAACTGAA GAGATCGAAT GAGAAGAACC CCGGGGAACT TGCCCAGCAC 
AAGGAAGACC CCAGGACTGA TGTCAAGGCA TACGATGTGA CACGGATGGT GTCCATGCCC 
CAGACGACAG CAGGCACCAT CCTGGACGGA GTGAACGTCG GCCGTGGCTA TGGCCTGGTG 
GAAGGACACG ACAGGAGGCA GTTTGAGATC ACCAGCGTTT CCGTGGATGT CTGGCACATC 
CTGGAATTCG ACTATAGCAG GCTCCCCAAA CAAAGCATCG GGCAGTTCCA TGAGGGGGAT 
GCCTATGTGG TCAAGTGGAA GTTCATGGTG AGCACGGCAG TGGGAAGTCG CCAGAAGGGA 
GAGCACTCGG TGAGGGCAGC CGGCAAAGAG AAGTGCGTCT ACTTCTTCTG GCAAGGCCGG 
CACTCCACCG TGAGTGAGAA GGGCACGTCG GCGCTGATGA CGGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT TCTCCAGGGA AAGGAGCCCC CCTGTTTCCT GCAGTGTTTC 
CAGGGGGGGA TGGTGGTGCA CTCGGGGAGG CGGGAAGAGG AAGAAGAAAA TGTGCAAAGT 
GAGTGGCGGC TGTACTGCGT GCGTGGAGAG GTGCCCGTGG AAGGGAATTT GCTGGAAGTG 
GCCTGTCACT GTAGCAGCCT GAGGTCCAGA ACTTCCATGG TGGTGCTTAA CGTCAACAAG 
GCCCTCATCT ACCTGTGGCA CGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGAAGG 
ACCGCTGCGA ACAAGATCAA GGAACAATGT CCCCTGGAAG CAGGACTGCA TAGTAGCAGC 
AAAGTCACAA TACACGAGTG TGATGAAGGC TCCGAGCCAC TCGGATTCTG GGATGCCTTA 
GGAAGGAGAG ACAGGAAAGC CTACGATTGC ATGCTTCAAG ATCCTGGAAG TTTTAACTTC 
GCGCCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGGGATT TTGCAGCCAC AGAGTTTGTG 
TACCCTGCCC GAGCCCCCTC TGTGGTCAGT TCCATGCCCT TCCTGCAGGA AGATCTGTAC 
AGCGCGCCCC AGCCAGCACT TTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACT GGTTCCGCCC GCATCCGCTG GGCCTCCGAC 
CGGAAGAGTG CGATGGAGAC TGTGCTCCAG TACTGCAAAG GAAAAAATCT CAAGAAACCA 
GCCCCCAAGT CTTACCTTAT CCACGCTGGT CTGGAGCCCC TGACATTCAC CAATATGTTT 
CCCAGCTGGG AGCACAGAGA GGACATCGCT GAGATCACAG AGATGGACAC GGAAGTTTCC 
AATCAGATCA CCCTCGTGGA AGACGTCTTA GCCAAGCTCT GTAAAACCAT TTACCCGCTG 
GCCGACCTCC TGGCCAGGCC ACTCCCGGAG GGGGTCGATC CTCTGAAGCT TGAGATCTAT 
CTCACCGACG AAGACTTCGA GTTTGCACTA GACATGACGA GGGATGAATA CAACGCCCTG 
CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA GCAAAAGGCC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517932.2
CDS	1241..8188
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519630.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519630.2

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CTGAACCTAT CCCCGCTTCG 
TGTTCTGGGG GCACCCACCC TGTAATGGCG AGACTTCCTA GCCCCACTGT AGCTAGGAGC 
GCTGTGCAGC CTGCCAGATT GCAGGCCTCT GCTCACCAAA AGGCCTTAGC CAAGGACCAG 
ACAAATGAGG GCAAAGAGCT TGCTGAGCAA GGAGAACCTG ATTCCTCCAC TCTAAGCTTG 
GCCGAAAAGT TGGCCTTGTT TAACAAATTG TCCCAGCCAG TCTCAAAAGC GATTTCTACC 
CGGAACAGAA TAGACACGAG ACAGAGGAGA ATGAACGCTC GCTATCAAAC TCAGCCAGTC 
ACACTGGGAG AGGTGGAGCA GGTGCAGAGT GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG 
AACACATCAG TGTCTACCGT AGCATCCACG GTTGCTCCAA TGTATGCCGG AGATCTTCGC 
ACAAAGCCAC CTCTTGACCA CAATGCAAGT GCCACTGACT ATAAGTTTTC TTCTTCAATA 
GAAAATTCGG ACTCTCCAGT TAGAAGCATT CTGAAATCGC AAGCTTGGCA GCCTTTGGTA 
GAGGGTAGCG AGAACAAGGG AATGTTGAGA GAATATGGAG AGACAGAAAG CAAGAGAGCT 
TTGACAGGTC GAGACAGTGG GATGGAGAAG TATGGGTCCT TTGAGGAAGC AGAAGCATCC 
TACCCCATCC TGAACCGAGC CAGGGAAGGA GACAGCCATA AGGAATCTAA ATATGCTGTT 
CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC CCTCCCATCA CCCACCTCGG GGATGAACCG 
AAGGAATTTT CCATGGCTAA AATGAATGCA CAAGGAAACT TGGACTTGAG GGACAGGCTG 
CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT GTTATGAAAA GGAAGTTTTC ACTAAGAGCG 
GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG ACGGGGACAG CTGCTGGGAA AACTATTGCT 
CAAACCACAG CCCCCGTGTC CTGGAAGCCC CAGGATTCTT CGGAACAGCC ACAGGAGAAG 
CTCTGCAAGA ATCCATGTGC GATGTTTGCT GCTGGAGAGA TCAAAACGCC GACAGGGGAG 
GGCCTTCTTG ACTCACCCAG CAAAACCATG TCTATTAAAG AAAGATTGGC ACTGTTGAAG 
AAAAGCGGGG AGGAAGATTG GAGAAACAGA CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG 
CCGGCCAGCA GCCTGCACAC CCAGGAAGCA GGGCGGTCCC TCATCAAGAA GGAAGAAGGA 
GGAGTTGCGG ATGATAGTGC CATTTCTAAT CTGCTTTGGC GGGTCACAGA AAGTCGAGAG 
AGCCAAATGA CGATTGAGGA GAGGAAGCAG CTCATCACTG TGAGAGAGGA GGCCTGGAAG 
ACGAGAGGCA GAGGAGCGGC CAACGACTCG ACCCAGTTCA CTGTGGCTGG CAGGATGGTG 
AAGAAAGGTT TGGCGTCACC TACTGCCATA ACCCCAGTAG CCTCACCCAT TTGCGGTAAA 
ACAAGAGGCA CCACACCCGT TTCCAAACCC CTGGAAGATA TCGAAGCCAG ACCAGATATG 
CAGTTAGAAT CGGACCTGAA GTTGGACAGG CTGGAAACCT TTCTAAGAAG GCTGAATAAC 
AAAGTTGGCG GGATGCACGA AACGGTGCTC ACTGTCACCG GCAAATCTGT GAAGGAGGTG 
ATGAAGCCAG ATGATGATGA AACCTTTGCC AAATTTTACC GCAGCGTGGA TTATAATATG 
CCAAGAAGTC CTGTGGAGAT GGATGAGGAC TTCGATGTCA TTTTCGATCC TTATGCACCC 
AAATTGACGT CTTCCGTGGC CGAGCACAAG CGGGCAGTTA GGCCCAAGCG CCGGGTTCAG 
GCCTCCAAAA ACCCCCTGAA AATGCTGGCG GCAAGAGAAG ATCTCCTTCA GGAATACACT 
GAGCAGAGAT TAAACGTTGC CTTCATGGAG TCAAAGCGGA TGAAAGTAGA AAAGATGTCT 
TCCAACTCCA ACTTCTCAGA AGTCACCCTG GCGGGTTTAG CCAGTAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAACT CTAACAACAG CGCCGTGCCC 
TACAAGAGGC TGATGCTGTT GCAGATTAAA GGAAGAAGAC ATGTGCAGAC CAGGCTGGTG 
GAACCTCGAG CTTCGGCGCT CAACAGTGGG GACTGCTTCC TCCTGCTCTC TCCCCACTGC 
TGCTTCCTGT GGGTAGGAGA GTTTGCAAAC GTCATAGAAA AGGCGAAGGC CTCAGAACTT 
GCAACTTTAA TTCAGACAAA GAGGGAACTT GGTTGTAGAG CTACTTATAT CCAAACCATT 
GAAGAAGGAA TTAATACACA CACTCATGCA GCCAAAGACT TCTGGAAGCT TCTGGGTGGC 
CAAACCAGTT ACCAATCTGC TGGAGACCCA AAAGAAGATG AACTCTATGA AGCAGCCATA 
ATAGAAACTA ACTGCATTTA CCGTCTCATG GATGACAAAC TTGTTCCTGA TGACGACTAC 
TGGGGGAAAA TTCCGAAGTG CTCCCTTCTG CAACCCAAAG AGGTACTGGT GTTTGATTTT 
GGTAGTGAAG TTTACGTATG GCATGGGAAA GAAGTCACAT TAGCACAACG AAAAATAGCA 
TTTCAGCTGG CAAAGCACTT ATGGAATGGA ACCTTTGACT ATGAGAACTG TGACATCAAT 
CCCCTGGATC CTGGAGAATG CAATCCGCTT ATCCCCAGAA AAGGACAGGG GCGGCCCGAC 
TGGGCGATAT TTGGGAGACT TACTGAACAC AATGAGACGA TTTTGTTCAA AGAGAAGTTT 
CTGGATTGGA CGGAACTGAA GAGATCGAAT GAGAAGAACC CCGGGGAACT TGCCCAGCAC 
AAGGAAGACC CCAGGACTGA TGTCAAGGCA TACGATGTGA CACGGATGGT GTCCATGCCC 
CAGACGACAG CAGGCACCAT CCTGGACGGA GTGAACGTCG GCCGTGGCTA TGGCCTGGTG 
GAAGGACACG ACAGGAGGCA GTTTGAGATC ACCAGCGTTT CCGTGGATGT CTGGCACATC 
CTGGAATTCG ACTATAGCAG GCTCCCCAAA CAAAGCATCG GGCAGTTCCA TGAGGGGGAT 
GCCTATGTGG TCAAGTGGAA GTTCATGGTG AGCACGGCAG TGGGAAGTCG CCAGAAGGGA 
GAGCACTCGG TGAGGGCAGC CGGCAAAGAG AAGTGCGTCT ACTTCTTCTG GCAAGGCCGG 
CACTCCACCG TGAGTGAGAA GGGCACGTCG GCGCTGATGA CGGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT TCTCCAGGGA AAGGAGCCCC CCTGTTTCCT GCAGTGTTTC 
CAGGGGGGGA TGGTGGTGCA CTCGGGGAGG CGGGAAGAGG AAGAAGAAAA TGTGCAAAGT 
GAGTGGCGGC TGTACTGCGT GCGTGGAGAG GTGCCCGTGG AAGGGAATTT GCTGGAAGTG 
GCCTGTCACT GTAGCAGCCT GAGGTCCAGA ACTTCCATGG TGGTGCTTAA CGTCAACAAG 
GCCCTCATCT ACCTGTGGCA CGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGAAGG 
ACCGCTGCGA ACAAGATCAA GGAACAATGT CCCCTGGAAG CAGGACTGCA TAGTAGCAGC 
AAAGTCACAA TACACGAGTG TGATGAAGGC TCCGAGCCAC TCGGATTCTG GGATGCCTTA 
GGAAGGAGAG ACAGGAAAGC CTACGATTGC ATGCTTCAAG ATCCTGGAAG TTTTAACTTC 
GCGCCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGGGATT TTGCAGCCAC AGAGTTTGTG 
TACCCTGCCC GAGCCCCCTC TGTGGTCAGT TCCATGCCCT TCCTGCAGGA AGATCTGTAC 
AGCGCGCCCC AGCCAGCACT TTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACT GGTTCCGCCC GCATCCGCTG GGCCTCCGAC 
CGGAAGAGTG CGATGGAGAC TGTGCTCCAG TACTGCAAAG GAAAAAATCT CAAGAAACCA 
GCCCCCAAGT CTTACCTTAT CCACGCTGGT CTGGAGCCCC TGACATTCAC CAATATGTTT 
CCCAGCTGGG AGCACAGAGA GGACATCGCT GAGATCACAG AGATGGACAC GGAAGTTTCC 
AATCAGATCA CCCTCGTGGA AGACGTCTTA GCCAAGCTCT GTAAAACCAT TTACCCGCTG 
GCCGACCTCC TGGCCAGGCC ACTCCCGGAG GGGGTCGATC CTCTGAAGCT TGAGATCTAT 
CTCACCGACG AAGACTTCGA GTTTGCACTA GACATGACGA GGGATGAATA CAACGCCCTG 
CCCGCCTGGA AGCAGGTGAA CCTGAAGAAA GCAAAAGGCC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_017016576.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872065.1 Homo sapiens supervillin (SVIL), transcript variant X3, mRNA.

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CloneID	OHu91599
Clone ID Related Accession (Same CDS sequence)	XM_017016576.1
Accession Version	XM_017016576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGAGTTAAAG TTAGAGAAAA ATTGGTGAAA GAGGAAAGTG CTCGAAACAG CCCTGAACTC 
GCCTCAGAGT CCGTAACTCA GAGGAGACAC CAGCCAGCGC CAGTCCATTA CGTGTCATTT 
CAGTCTGAGC ACTCAGCCTT TGATAGGGTC CCCAGCAAGG CAGCAGGCTC TACACGACAG 
CCAATCCGTG GCTATGTCCA ACCCGCAGAT ACCGGTCACA CCGCCAAGCT AGTGACGCCA 
GAAACCCCAG AAAATGCATC TGAGTGTAGC TGGGTAGCAT CAGCCACCCA GAATGTCCCC 
AAACCTCCCA GCTTGACGGT TCTAGAAGGT GACGGAAGGG ATAGCCCAGT TCTCCATGTC 
TGCGAGTCAA AAGCAGAAGA AGAAGAAGGG GAAGGAGAAG GAGAAGAAAA AGAAGAAGAT 
GTGTGCTTCA CTGAAGCTCT CGAGCAAAGC AAGAAAACCC TACTGGCTTT GGAGGGTGAT 
GGGCTAGTGA GAAGCCCAGA AGATCCCTCT AGAAATGAGG ACTTTGGTAA GCCTGCTGTG 
AGCACAGTCA CCTTAGAGCA TCAGAAGGAA CTGGAAAACG TGGCACAACC CCCTCAAGCT 
CCGCACCAGC CCACTGAGAG GACAGGCAGG AGCGAGATGG TTCTCTACAT TCAAAGTGAG 
CCTGTGTCCC AAGACGCCAA ACCAACTGGT CACAACAGGG AAGCCTCGAA AAAGCGCAAG 
GTCCGTACCC GCTCTCTGTC AGATTTCACA GGCCCCCCTC AGCTCCAGGC CTTGAAGTAT 
AAGGACCCAG CTTCCAGGAG AGAGCTGGAG CTGCCCAGCT CCAAGACCGA AGGGCCTTAT 
GGGGAGATCA GCATGCTGGA CACAAAAGTC TCTGTCGCCC AGCTCCGAAG TGCGTTCCTG 
GCATCTGCCA ACGCCTGCAG GAGACCTGAA CTCAAATCAC GGGTGGAGAG GTCGGCTGAA 
GGACCTGGCT TGCCCACCGG TGTGGAACGG GAGAGAGGGT CCCGGAAACC AAGACGCTAT 
TTTTCTCCTG GTGAAAGTAG AAAAACTTCC GAGAGATTTA GAACCCAACC TATAACTTCA 
GCAGAACGAA AGGAATCGGA TAGGTGCACT TCACATTCAG AAACGCCAAC TGTCGATGAT 
GAAGAAAAGG TGGATGAACG AGCCAAGCTG AGCGTCGCCG CCAAGAGGTT GCTTTTCAGG 
GAGATGGAAA AATCTTTTGA TGAACAAAAT GTTCCAAAGC GACGCTCAAG AAACACAGCT 
GTGGAGCAGA GGCTACGCCG TCTGCAGGAC AGGTCCCTCA CCCAGCCCAT CACCACTGAA 
GAGGTGGTCA TCGCAGCCAC TGAACCTATC CCCGCTTCGT GTTCTGGGGG CACCCACCCT 
GTAATGGCGA GACTTCCTAG CCCCACTGTA GCTAGGAGCG CTGTGCAGCC TGCCAGATTG 
CAGGCCTCTG CTCACCAAAA GGCCTTAGCC AAGGACCAGA CAAATGAGGG CAAAGAGCTT 
GCTGAGCAAG GAGAACCTGA TTCCTCCACT CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT 
AACAAATTGT CCCAGCCAGT CTCAAAAGCG ATTTCTACCC GGAACAGAAT AGACACGAGA 
CAGAGGAGAA TGAACGCTCG CTATCAAACT CAGCCAGTCA CACTGGGAGA GGTGGAGCAG 
GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA CCTGCCGTGA ACACATCAGT GTCTACCGTA 
GCATCCACGG TTGCTCCAAT GTATGCCGGA GATCTTCGCA CAAAGCCACC TCTTGACCAC 
AATGCAAGTG CCACTGACTA TAAGTTTTCT TCTTCAATAG AAAATTCGGA CTCTCCAGTT 
AGAAGCATTC TGAAATCGCA AGCTTGGCAG CCTTTGGTAG AGGGTAGCGA GAACAAGGGA 
ATGTTGAGAG AATATGGAGA GACAGAAAGC AAGAGAGCTT TGACAGGTCG AGACAGTGGG 
ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA GAAGCATCCT ACCCCATCCT GAACCGAGCC 
AGGGAAGGAG ACAGCCATAA GGAATCTAAA TATGCTGTTC CCAGAAGAGG AAGCCTGGAA 
CGGGCGAACC CTCCCATCAC CCACCTCGGG GATGAACCGA AGGAATTTTC CATGGCTAAA 
ATGAATGCAC AAGGAAACTT GGACTTGAGG GACAGGCTGC CCTTTGAAGA GAAGGTGGAG 
GTGGAGAATG TTATGAAAAG GAAGTTTTCA CTAAGAGCGG CAGAGTTCGG GGAGCCCACT 
TCCGAGCAGA CGGGGACAGC TGCTGGGAAA ACTATTGCTC AAACCACAGC CCCCGTGTCC 
TGGAAGCCCC AGGATTCTTC GGAACAGCCA CAGGAGAAGC TCTGCAAGAA TCCATGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAACGCCG ACAGGGGAGG GCCTTCTTGA CTCACCCAGC 
AAAACCATGT CTATTAAAGA AAGATTGGCA CTGTTGAAGA AAAGCGGGGA GGAAGATTGG 
AGAAACAGAC TCAGCAGGAG GCAGGAGGGC GGCAAGGCGC CGGCCAGCAG CCTGCACACC 
CAGGAAGCAG GGCGGTCCCT CATCAAGAAG GAAGAAGGAG GAGTTGCGGA TGATAGTGCC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAT GCTTCTACTT ATTCTCCTGC TATTCCTGCT 
GCCCATAAAT ACCTGTCTTT TGTGTCGATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872065.1
CDS	1241..8185
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_017016576.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016576.1

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGAGTTAAAG TTAGAGAAAA ATTGGTGAAA GAGGAAAGTG CTCGAAACAG CCCTGAACTC 
GCCTCAGAGT CCGTAACTCA GAGGAGACAC CAGCCAGCGC CAGTCCATTA CGTGTCATTT 
CAGTCTGAGC ACTCAGCCTT TGATAGGGTC CCCAGCAAGG CAGCAGGCTC TACACGACAG 
CCAATCCGTG GCTATGTCCA ACCCGCAGAT ACCGGTCACA CCGCCAAGCT AGTGACGCCA 
GAAACCCCAG AAAATGCATC TGAGTGTAGC TGGGTAGCAT CAGCCACCCA GAATGTCCCC 
AAACCTCCCA GCTTGACGGT TCTAGAAGGT GACGGAAGGG ATAGCCCAGT TCTCCATGTC 
TGCGAGTCAA AAGCAGAAGA AGAAGAAGGG GAAGGAGAAG GAGAAGAAAA AGAAGAAGAT 
GTGTGCTTCA CTGAAGCTCT CGAGCAAAGC AAGAAAACCC TACTGGCTTT GGAGGGTGAT 
GGGCTAGTGA GAAGCCCAGA AGATCCCTCT AGAAATGAGG ACTTTGGTAA GCCTGCTGTG 
AGCACAGTCA CCTTAGAGCA TCAGAAGGAA CTGGAAAACG TGGCACAACC CCCTCAAGCT 
CCGCACCAGC CCACTGAGAG GACAGGCAGG AGCGAGATGG TTCTCTACAT TCAAAGTGAG 
CCTGTGTCCC AAGACGCCAA ACCAACTGGT CACAACAGGG AAGCCTCGAA AAAGCGCAAG 
GTCCGTACCC GCTCTCTGTC AGATTTCACA GGCCCCCCTC AGCTCCAGGC CTTGAAGTAT 
AAGGACCCAG CTTCCAGGAG AGAGCTGGAG CTGCCCAGCT CCAAGACCGA AGGGCCTTAT 
GGGGAGATCA GCATGCTGGA CACAAAAGTC TCTGTCGCCC AGCTCCGAAG TGCGTTCCTG 
GCATCTGCCA ACGCCTGCAG GAGACCTGAA CTCAAATCAC GGGTGGAGAG GTCGGCTGAA 
GGACCTGGCT TGCCCACCGG TGTGGAACGG GAGAGAGGGT CCCGGAAACC AAGACGCTAT 
TTTTCTCCTG GTGAAAGTAG AAAAACTTCC GAGAGATTTA GAACCCAACC TATAACTTCA 
GCAGAACGAA AGGAATCGGA TAGGTGCACT TCACATTCAG AAACGCCAAC TGTCGATGAT 
GAAGAAAAGG TGGATGAACG AGCCAAGCTG AGCGTCGCCG CCAAGAGGTT GCTTTTCAGG 
GAGATGGAAA AATCTTTTGA TGAACAAAAT GTTCCAAAGC GACGCTCAAG AAACACAGCT 
GTGGAGCAGA GGCTACGCCG TCTGCAGGAC AGGTCCCTCA CCCAGCCCAT CACCACTGAA 
GAGGTGGTCA TCGCAGCCAC TGAACCTATC CCCGCTTCGT GTTCTGGGGG CACCCACCCT 
GTAATGGCGA GACTTCCTAG CCCCACTGTA GCTAGGAGCG CTGTGCAGCC TGCCAGATTG 
CAGGCCTCTG CTCACCAAAA GGCCTTAGCC AAGGACCAGA CAAATGAGGG CAAAGAGCTT 
GCTGAGCAAG GAGAACCTGA TTCCTCCACT CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT 
AACAAATTGT CCCAGCCAGT CTCAAAAGCG ATTTCTACCC GGAACAGAAT AGACACGAGA 
CAGAGGAGAA TGAACGCTCG CTATCAAACT CAGCCAGTCA CACTGGGAGA GGTGGAGCAG 
GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA CCTGCCGTGA ACACATCAGT GTCTACCGTA 
GCATCCACGG TTGCTCCAAT GTATGCCGGA GATCTTCGCA CAAAGCCACC TCTTGACCAC 
AATGCAAGTG CCACTGACTA TAAGTTTTCT TCTTCAATAG AAAATTCGGA CTCTCCAGTT 
AGAAGCATTC TGAAATCGCA AGCTTGGCAG CCTTTGGTAG AGGGTAGCGA GAACAAGGGA 
ATGTTGAGAG AATATGGAGA GACAGAAAGC AAGAGAGCTT TGACAGGTCG AGACAGTGGG 
ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA GAAGCATCCT ACCCCATCCT GAACCGAGCC 
AGGGAAGGAG ACAGCCATAA GGAATCTAAA TATGCTGTTC CCAGAAGAGG AAGCCTGGAA 
CGGGCGAACC CTCCCATCAC CCACCTCGGG GATGAACCGA AGGAATTTTC CATGGCTAAA 
ATGAATGCAC AAGGAAACTT GGACTTGAGG GACAGGCTGC CCTTTGAAGA GAAGGTGGAG 
GTGGAGAATG TTATGAAAAG GAAGTTTTCA CTAAGAGCGG CAGAGTTCGG GGAGCCCACT 
TCCGAGCAGA CGGGGACAGC TGCTGGGAAA ACTATTGCTC AAACCACAGC CCCCGTGTCC 
TGGAAGCCCC AGGATTCTTC GGAACAGCCA CAGGAGAAGC TCTGCAAGAA TCCATGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAACGCCG ACAGGGGAGG GCCTTCTTGA CTCACCCAGC 
AAAACCATGT CTATTAAAGA AAGATTGGCA CTGTTGAAGA AAAGCGGGGA GGAAGATTGG 
AGAAACAGAC TCAGCAGGAG GCAGGAGGGC GGCAAGGCGC CGGCCAGCAG CCTGCACACC 
CAGGAAGCAG GGCGGTCCCT CATCAAGAAG GAAGAAGGAG GAGTTGCGGA TGATAGTGCC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAT GCTTCTACTT ATTCTCCTGC TATTCCTGCT 
GCCCATAAAT ACCTGTCTTT TGTGTCGATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519631.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517933.2 Homo sapiens supervillin (SVIL), transcript variant X4, mRNA.

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CloneID	OHu91600
Clone ID Related Accession (Same CDS sequence)	XM_011519631.2
Accession Version	XM_011519631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519631.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CATTGCAGGC CTCTGCTCAC 
CAAAAGGCCT TAGCCAAGGA CCAGACAAAT GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA 
CCTGATTCCT CCACTCTAAG CTTGGCCGAA AAGTTGGCCT TGTTTAACAA ATTGTCCCAG 
CCAGTCTCAA AAGCGATTTC TACCCGGAAC AGAATAGACA CGAGACAGAG GAGAATGAAC 
GCTCGCTATC AAACTCAGCC AGTCACACTG GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG 
CTCATTCCTT TCTCACCTGC CGTGAACACA TCAGTGTCTA CCGTAGCATC CACGGTTGCT 
CCAATGTATG CCGGAGATCT TCGCACAAAG CCACCTCTTG ACCACAATGC AAGTGCCACT 
GACTATAAGT TTTCTTCTTC AATAGAAAAT TCGGACTCTC CAGTTAGAAG CATTCTGAAA 
TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT AGCGAGAACA AGGGAATGTT GAGAGAATAT 
GGAGAGACAG AAAGCAAGAG AGCTTTGACA GGTCGAGACA GTGGGATGGA GAAGTATGGG 
TCCTTTGAGG AAGCAGAAGC ATCCTACCCC ATCCTGAACC GAGCCAGGGA AGGAGACAGC 
CATAAGGAAT CTAAATATGC TGTTCCCAGA AGAGGAAGCC TGGAACGGGC GAACCCTCCC 
ATCACCCACC TCGGGGATGA ACCGAAGGAA TTTTCCATGG CTAAAATGAA TGCACAAGGA 
AACTTGGACT TGAGGGACAG GCTGCCCTTT GAAGAGAAGG TGGAGGTGGA GAATGTTATG 
AAAAGGAAGT TTTCACTAAG AGCGGCAGAG TTCGGGGAGC CCACTTCCGA GCAGACGGGG 
ACAGCTGCTG GGAAAACTAT TGCTCAAACC ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT 
TCTTCGGAAC AGCCACAGGA GAAGCTCTGC AAGAATCCAT GTGCGATGTT TGCTGCTGGA 
GAGATCAAAA CGCCGACAGG GGAGGGCCTT CTTGACTCAC CCAGCAAAAC CATGTCTATT 
AAAGAAAGAT TGGCACTGTT GAAGAAAAGC GGGGAGGAAG ATTGGAGAAA CAGACTCAGC 
AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC AGCAGCCTGC ACACCCAGGA AGCAGGGCGG 
TCCCTCATCA AGAAGGAAGA AGGAGGAGTT GCGGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATTC CTGCTGCCCA TAAATACCTG 
TCTTTTGTGT CGATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAAAT GACGATTGAG 
GAGAGGAAGC AGCTCATCAC TGTGAGAGAG GAGGCCTGGA AGACGAGAGG CAGAGGAGCG 
GCCAACGACT CGACCCAGTT CACTGTGGCT GGCAGGATGG TGAAGAAAGG TTTGGCGTCA 
CCTACTGCCA TAACCCCAGT AGCCTCACCC ATTTGCGGTA AAACAAGAGG CACCACACCC 
GTTTCCAAAC CCCTGGAAGA TATCGAAGCC AGACCAGATA TGCAGTTAGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG CGGGATGCAC 
GAAACGGTGC TCACTGTCAC CGGCAAATCT GTGAAGGAGG TGATGAAGCC AGATGATGAT 
GAAACCTTTG CCAAATTTTA CCGCAGCGTG GATTATAATA TGCCAAGAAG TCCTGTGGAG 
ATGGATGAGG ACTTCGATGT CATTTTCGAT CCTTATGCAC CCAAATTGAC GTCTTCCGTG 
GCCGAGCACA AGCGGGCAGT TAGGCCCAAG CGCCGGGTTC AGGCCTCCAA AAACCCCCTG 
AAAATGCTGG CGGCAAGAGA AGATCTCCTT CAGGAATACA CTGAGCAGAG ATTAAACGTT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CTTCCAACTC CAACTTCTCA 
GAAGTCACCC TGGCGGGTTT AGCCAGTAAA GAAAACTTCA GCAACGTCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA CTCTAACAAC AGCGCCGTGC CCTACAAGAG GCTGATGCTG 
TTGCAGATTA AAGGAAGAAG ACATGTGCAG ACCAGGCTGG TGGAACCTCG AGCTTCGGCG 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCTCCCCACT GCTGCTTCCT GTGGGTAGGA 
GAGTTTGCAA ACGTCATAGA AAAGGCGAAG GCCTCAGAAC TTGCAACTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACTTAT ATCCAAACCA TTGAAGAAGG AATTAATACA 
CACACTCATG CAGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAATCT 
GCTGGAGACC CAAAAGAAGA TGAACTCTAT GAAGCAGCCA TAATAGAAAC TAACTGCATT 
TACCGTCTCA TGGATGACAA ACTTGTTCCT GATGACGACT ACTGGGGGAA AATTCCGAAG 
TGCTCCCTTC TGCAACCCAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTTTACGTA 
TGGCATGGGA AAGAAGTCAC ATTAGCACAA CGAAAAATAG CATTTCAGCT GGCAAAGCAC 
TTATGGAATG GAACCTTTGA CTATGAGAAC TGTGACATCA ATCCCCTGGA TCCTGGAGAA 
TGCAATCCGC TTATCCCCAG AAAAGGACAG GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA 
CTTACTGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAGT TTCTGGATTG GACGGAACTG 
AAGAGATCGA ATGAGAAGAA CCCCGGGGAA CTTGCCCAGC ACAAGGAAGA CCCCAGGACT 
GATGTCAAGG CATACGATGT GACACGGATG GTGTCCATGC CCCAGACGAC AGCAGGCACC 
ATCCTGGACG GAGTGAACGT CGGCCGTGGC TATGGCCTGG TGGAAGGACA CGACAGGAGG 
CAGTTTGAGA TCACCAGCGT TTCCGTGGAT GTCTGGCACA TCCTGGAATT CGACTATAGC 
AGGCTCCCCA AACAAAGCAT CGGGCAGTTC CATGAGGGGG ATGCCTATGT GGTCAAGTGG 
AAGTTCATGG TGAGCACGGC AGTGGGAAGT CGCCAGAAGG GAGAGCACTC GGTGAGGGCA 
GCCGGCAAAG AGAAGTGCGT CTACTTCTTC TGGCAAGGCC GGCACTCCAC CGTGAGTGAG 
AAGGGCACGT CGGCGCTGAT GACGGTGGAG CTGGACGAGG AAAGGGGGGC CCAGGTCCAG 
GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC CTGCAGTGTT TCCAGGGGGG GATGGTGGTG 
CACTCGGGGA GGCGGGAAGA GGAAGAAGAA AATGTGCAAA GTGAGTGGCG GCTGTACTGC 
GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC 
CTGAGGTCCA GAACTTCCAT GGTGGTGCTT AACGTCAACA AGGCCCTCAT CTACCTGTGG 
CACGGATGCA AAGCCCAGGC CCACACGAAG GAGGTCGGAA GGACCGCTGC GAACAAGATC 
AAGGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTCAC AATACACGAG 
TGTGATGAAG GCTCCGAGCC ACTCGGATTC TGGGATGCCT TAGGAAGGAG AGACAGGAAA 
GCCTACGATT GCATGCTTCA AGATCCTGGA AGTTTTAACT TCGCGCCCCG CCTGTTCATC 
CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC ACAGAGTTTG TGTACCCTGC CCGAGCCCCC 
TCTGTGGTCA GTTCCATGCC CTTCCTGCAG GAAGATCTGT ACAGCGCGCC CCAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTG TACCTCTGGC AAGGCTGGTG GCCCATCGAG 
AACAAGATCA CTGGTTCCGC CCGCATCCGC TGGGCCTCCG ACCGGAAGAG TGCGATGGAG 
ACTGTGCTCC AGTACTGCAA AGGAAAAAAT CTCAAGAAAC CAGCCCCCAA GTCTTACCTT 
ATCCACGCTG GTCTGGAGCC CCTGACATTC ACCAATATGT TTCCCAGCTG GGAGCACAGA 
GAGGACATCG CTGAGATCAC AGAGATGGAC ACGGAAGTTT CCAATCAGAT CACCCTCGTG 
GAAGACGTCT TAGCCAAGCT CTGTAAAACC ATTTACCCGC TGGCCGACCT CCTGGCCAGG 
CCACTCCCGG AGGGGGTCGA TCCTCTGAAG CTTGAGATCT ATCTCACCGA CGAAGACTTC 
GAGTTTGCAC TAGACATGAC GAGGGATGAA TACAACGCCC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517933.2
CDS	1241..8170
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519631.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519631.2

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CATTGCAGGC CTCTGCTCAC 
CAAAAGGCCT TAGCCAAGGA CCAGACAAAT GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA 
CCTGATTCCT CCACTCTAAG CTTGGCCGAA AAGTTGGCCT TGTTTAACAA ATTGTCCCAG 
CCAGTCTCAA AAGCGATTTC TACCCGGAAC AGAATAGACA CGAGACAGAG GAGAATGAAC 
GCTCGCTATC AAACTCAGCC AGTCACACTG GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG 
CTCATTCCTT TCTCACCTGC CGTGAACACA TCAGTGTCTA CCGTAGCATC CACGGTTGCT 
CCAATGTATG CCGGAGATCT TCGCACAAAG CCACCTCTTG ACCACAATGC AAGTGCCACT 
GACTATAAGT TTTCTTCTTC AATAGAAAAT TCGGACTCTC CAGTTAGAAG CATTCTGAAA 
TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT AGCGAGAACA AGGGAATGTT GAGAGAATAT 
GGAGAGACAG AAAGCAAGAG AGCTTTGACA GGTCGAGACA GTGGGATGGA GAAGTATGGG 
TCCTTTGAGG AAGCAGAAGC ATCCTACCCC ATCCTGAACC GAGCCAGGGA AGGAGACAGC 
CATAAGGAAT CTAAATATGC TGTTCCCAGA AGAGGAAGCC TGGAACGGGC GAACCCTCCC 
ATCACCCACC TCGGGGATGA ACCGAAGGAA TTTTCCATGG CTAAAATGAA TGCACAAGGA 
AACTTGGACT TGAGGGACAG GCTGCCCTTT GAAGAGAAGG TGGAGGTGGA GAATGTTATG 
AAAAGGAAGT TTTCACTAAG AGCGGCAGAG TTCGGGGAGC CCACTTCCGA GCAGACGGGG 
ACAGCTGCTG GGAAAACTAT TGCTCAAACC ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT 
TCTTCGGAAC AGCCACAGGA GAAGCTCTGC AAGAATCCAT GTGCGATGTT TGCTGCTGGA 
GAGATCAAAA CGCCGACAGG GGAGGGCCTT CTTGACTCAC CCAGCAAAAC CATGTCTATT 
AAAGAAAGAT TGGCACTGTT GAAGAAAAGC GGGGAGGAAG ATTGGAGAAA CAGACTCAGC 
AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC AGCAGCCTGC ACACCCAGGA AGCAGGGCGG 
TCCCTCATCA AGAAGGAAGA AGGAGGAGTT GCGGATGATA GTGCCATTTC TAATCTGCTT 
TGGGAACCTG TATATGCTTC TACTTATTCT CCTGCTATTC CTGCTGCCCA TAAATACCTG 
TCTTTTGTGT CGATTAATCA GCGGGTCACA GAAAGTCGAG AGAGCCAAAT GACGATTGAG 
GAGAGGAAGC AGCTCATCAC TGTGAGAGAG GAGGCCTGGA AGACGAGAGG CAGAGGAGCG 
GCCAACGACT CGACCCAGTT CACTGTGGCT GGCAGGATGG TGAAGAAAGG TTTGGCGTCA 
CCTACTGCCA TAACCCCAGT AGCCTCACCC ATTTGCGGTA AAACAAGAGG CACCACACCC 
GTTTCCAAAC CCCTGGAAGA TATCGAAGCC AGACCAGATA TGCAGTTAGA ATCGGACCTG 
AAGTTGGACA GGCTGGAAAC CTTTCTAAGA AGGCTGAATA ACAAAGTTGG CGGGATGCAC 
GAAACGGTGC TCACTGTCAC CGGCAAATCT GTGAAGGAGG TGATGAAGCC AGATGATGAT 
GAAACCTTTG CCAAATTTTA CCGCAGCGTG GATTATAATA TGCCAAGAAG TCCTGTGGAG 
ATGGATGAGG ACTTCGATGT CATTTTCGAT CCTTATGCAC CCAAATTGAC GTCTTCCGTG 
GCCGAGCACA AGCGGGCAGT TAGGCCCAAG CGCCGGGTTC AGGCCTCCAA AAACCCCCTG 
AAAATGCTGG CGGCAAGAGA AGATCTCCTT CAGGAATACA CTGAGCAGAG ATTAAACGTT 
GCCTTCATGG AGTCAAAGCG GATGAAAGTA GAAAAGATGT CTTCCAACTC CAACTTCTCA 
GAAGTCACCC TGGCGGGTTT AGCCAGTAAA GAAAACTTCA GCAACGTCAG CCTGCGGAGC 
GTCAACCTGA CGGAACAGAA CTCTAACAAC AGCGCCGTGC CCTACAAGAG GCTGATGCTG 
TTGCAGATTA AAGGAAGAAG ACATGTGCAG ACCAGGCTGG TGGAACCTCG AGCTTCGGCG 
CTCAACAGTG GGGACTGCTT CCTCCTGCTC TCTCCCCACT GCTGCTTCCT GTGGGTAGGA 
GAGTTTGCAA ACGTCATAGA AAAGGCGAAG GCCTCAGAAC TTGCAACTTT AATTCAGACA 
AAGAGGGAAC TTGGTTGTAG AGCTACTTAT ATCCAAACCA TTGAAGAAGG AATTAATACA 
CACACTCATG CAGCCAAAGA CTTCTGGAAG CTTCTGGGTG GCCAAACCAG TTACCAATCT 
GCTGGAGACC CAAAAGAAGA TGAACTCTAT GAAGCAGCCA TAATAGAAAC TAACTGCATT 
TACCGTCTCA TGGATGACAA ACTTGTTCCT GATGACGACT ACTGGGGGAA AATTCCGAAG 
TGCTCCCTTC TGCAACCCAA AGAGGTACTG GTGTTTGATT TTGGTAGTGA AGTTTACGTA 
TGGCATGGGA AAGAAGTCAC ATTAGCACAA CGAAAAATAG CATTTCAGCT GGCAAAGCAC 
TTATGGAATG GAACCTTTGA CTATGAGAAC TGTGACATCA ATCCCCTGGA TCCTGGAGAA 
TGCAATCCGC TTATCCCCAG AAAAGGACAG GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA 
CTTACTGAAC ACAATGAGAC GATTTTGTTC AAAGAGAAGT TTCTGGATTG GACGGAACTG 
AAGAGATCGA ATGAGAAGAA CCCCGGGGAA CTTGCCCAGC ACAAGGAAGA CCCCAGGACT 
GATGTCAAGG CATACGATGT GACACGGATG GTGTCCATGC CCCAGACGAC AGCAGGCACC 
ATCCTGGACG GAGTGAACGT CGGCCGTGGC TATGGCCTGG TGGAAGGACA CGACAGGAGG 
CAGTTTGAGA TCACCAGCGT TTCCGTGGAT GTCTGGCACA TCCTGGAATT CGACTATAGC 
AGGCTCCCCA AACAAAGCAT CGGGCAGTTC CATGAGGGGG ATGCCTATGT GGTCAAGTGG 
AAGTTCATGG TGAGCACGGC AGTGGGAAGT CGCCAGAAGG GAGAGCACTC GGTGAGGGCA 
GCCGGCAAAG AGAAGTGCGT CTACTTCTTC TGGCAAGGCC GGCACTCCAC CGTGAGTGAG 
AAGGGCACGT CGGCGCTGAT GACGGTGGAG CTGGACGAGG AAAGGGGGGC CCAGGTCCAG 
GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC CTGCAGTGTT TCCAGGGGGG GATGGTGGTG 
CACTCGGGGA GGCGGGAAGA GGAAGAAGAA AATGTGCAAA GTGAGTGGCG GCTGTACTGC 
GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC 
CTGAGGTCCA GAACTTCCAT GGTGGTGCTT AACGTCAACA AGGCCCTCAT CTACCTGTGG 
CACGGATGCA AAGCCCAGGC CCACACGAAG GAGGTCGGAA GGACCGCTGC GAACAAGATC 
AAGGAACAAT GTCCCCTGGA AGCAGGACTG CATAGTAGCA GCAAAGTCAC AATACACGAG 
TGTGATGAAG GCTCCGAGCC ACTCGGATTC TGGGATGCCT TAGGAAGGAG AGACAGGAAA 
GCCTACGATT GCATGCTTCA AGATCCTGGA AGTTTTAACT TCGCGCCCCG CCTGTTCATC 
CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC ACAGAGTTTG TGTACCCTGC CCGAGCCCCC 
TCTGTGGTCA GTTCCATGCC CTTCCTGCAG GAAGATCTGT ACAGCGCGCC CCAGCCAGCA 
CTTTTCCTTG TTGACAATCA CCACGAGGTG TACCTCTGGC AAGGCTGGTG GCCCATCGAG 
AACAAGATCA CTGGTTCCGC CCGCATCCGC TGGGCCTCCG ACCGGAAGAG TGCGATGGAG 
ACTGTGCTCC AGTACTGCAA AGGAAAAAAT CTCAAGAAAC CAGCCCCCAA GTCTTACCTT 
ATCCACGCTG GTCTGGAGCC CCTGACATTC ACCAATATGT TTCCCAGCTG GGAGCACAGA 
GAGGACATCG CTGAGATCAC AGAGATGGAC ACGGAAGTTT CCAATCAGAT CACCCTCGTG 
GAAGACGTCT TAGCCAAGCT CTGTAAAACC ATTTACCCGC TGGCCGACCT CCTGGCCAGG 
CCACTCCCGG AGGGGGTCGA TCCTCTGAAG CTTGAGATCT ATCTCACCGA CGAAGACTTC 
GAGTTTGCAC TAGACATGAC GAGGGATGAA TACAACGCCC TGCCCGCCTG GAAGCAGGTG 
AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_017016577.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872066.1 Homo sapiens supervillin (SVIL), transcript variant X5, mRNA.

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CloneID	OHu91601
Clone ID Related Accession (Same CDS sequence)	XM_017016577.1
Accession Version	XM_017016577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CTGAACCTAT CCCCGCTTCG 
TGTTCTGGGG GCACCCACCC TGTAATGGCG AGACTTCCTA GCCCCACTGT AGCTAGGAGC 
GCTGTGCAGC CTGCCAGATT GCAGGCCTCT GCTCACCAAA AGGCCTTAGC CAAGGACCAG 
ACAAATGAGG GCAAAGAGCT TGCTGAGCAA GGAGAACCTG ATTCCTCCAC TCTAAGCTTG 
GCCGAAAAGT TGGCCTTGTT TAACAAATTG TCCCAGCCAG TCTCAAAAGC GATTTCTACC 
CGGAACAGAA TAGACACGAG ACAGAGGAGA ATGAACGCTC GCTATCAAAC TCAGCCAGTC 
ACACTGGGAG AGGTGGAGCA GGTGCAGAGT GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG 
AACACATCAG TGTCTACCGT AGCATCCACG GTTGCTCCAA TGTATGCCGG AGATCTTCGC 
ACAAAGCCAC CTCTTGACCA CAATGCAAGT GCCACTGACT ATAAGTTTTC TTCTTCAATA 
GAAAATTCGG ACTCTCCAGT TAGAAGCATT CTGAAATCGC AAGCTTGGCA GCCTTTGGTA 
GAGGGTAGCG AGAACAAGGG AATGTTGAGA GAATATGGAG AGACAGAAAG CAAGAGAGCT 
TTGACAGGTC GAGACAGTGG GATGGAGAAG TATGGGTCCT TTGAGGAAGC AGAAGCATCC 
TACCCCATCC TGAACCGAGC CAGGGAAGGA GACAGCCATA AGGAATCTAA ATATGCTGTT 
CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC CCTCCCATCA CCCACCTCGG GGATGAACCG 
AAGGAATTTT CCATGGCTAA AATGAATGCA CAAGGAAACT TGGACTTGAG GGACAGGCTG 
CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT GTTATGAAAA GGAAGTTTTC ACTAAGAGCG 
GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG ACGGGGACAG CTGCTGGGAA AACTATTGCT 
CAAACCACAG CCCCCGTGTC CTGGAAGCCC CAGGATTCTT CGGAACAGCC ACAGGAGAAG 
CTCTGCAAGA ATCCATGTGC GATGTTTGCT GCTGGAGAGA TCAAAACGCC GACAGGGGAG 
GGCCTTCTTG ACTCACCCAG CAAAACCATG TCTATTAAAG AAAGATTGGC ACTGTTGAAG 
AAAAGCGGGG AGGAAGATTG GAGAAACAGA CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG 
CCGGCCAGCA GCCTGCACAC CCAGGAAGCA GGGCGGTCCC TCATCAAGAA GCGGGTCACA 
GAAAGTCGAG AGAGCCAAAT GACGATTGAG GAGAGGAAGC AGCTCATCAC TGTGAGAGAG 
GAGGCCTGGA AGACGAGAGG CAGAGGAGCG GCCAACGACT CGACCCAGTT CACTGTGGCT 
GGCAGGATGG TGAAGAAAGG TTTGGCGTCA CCTACTGCCA TAACCCCAGT AGCCTCACCC 
ATTTGCGGTA AAACAAGAGG CACCACACCC GTTTCCAAAC CCCTGGAAGA TATCGAAGCC 
AGACCAGATA TGCAGTTAGA ATCGGACCTG AAGTTGGACA GGCTGGAAAC CTTTCTAAGA 
AGGCTGAATA ACAAAGTTGG CGGGATGCAC GAAACGGTGC TCACTGTCAC CGGCAAATCT 
GTGAAGGAGG TGATGAAGCC AGATGATGAT GAAACCTTTG CCAAATTTTA CCGCAGCGTG 
GATTATAATA TGCCAAGAAG TCCTGTGGAG ATGGATGAGG ACTTCGATGT CATTTTCGAT 
CCTTATGCAC CCAAATTGAC GTCTTCCGTG GCCGAGCACA AGCGGGCAGT TAGGCCCAAG 
CGCCGGGTTC AGGCCTCCAA AAACCCCCTG AAAATGCTGG CGGCAAGAGA AGATCTCCTT 
CAGGAATACA CTGAGCAGAG ATTAAACGTT GCCTTCATGG AGTCAAAGCG GATGAAAGTA 
GAAAAGATGT CTTCCAACTC CAACTTCTCA GAAGTCACCC TGGCGGGTTT AGCCAGTAAA 
GAAAACTTCA GCAACGTCAG CCTGCGGAGC GTCAACCTGA CGGAACAGAA CTCTAACAAC 
AGCGCCGTGC CCTACAAGAG GCTGATGCTG TTGCAGATTA AAGGAAGAAG ACATGTGCAG 
ACCAGGCTGG TGGAACCTCG AGCTTCGGCG CTCAACAGTG GGGACTGCTT CCTCCTGCTC 
TCTCCCCACT GCTGCTTCCT GTGGGTAGGA GAGTTTGCAA ACGTCATAGA AAAGGCGAAG 
GCCTCAGAAC TTGCAACTTT AATTCAGACA AAGAGGGAAC TTGGTTGTAG AGCTACTTAT 
ATCCAAACCA TTGAAGAAGG AATTAATACA CACACTCATG CAGCCAAAGA CTTCTGGAAG 
CTTCTGGGTG GCCAAACCAG TTACCAATCT GCTGGAGACC CAAAAGAAGA TGAACTCTAT 
GAAGCAGCCA TAATAGAAAC TAACTGCATT TACCGTCTCA TGGATGACAA ACTTGTTCCT 
GATGACGACT ACTGGGGGAA AATTCCGAAG TGCTCCCTTC TGCAACCCAA AGAGGTACTG 
GTGTTTGATT TTGGTAGTGA AGTTTACGTA TGGCATGGGA AAGAAGTCAC ATTAGCACAA 
CGAAAAATAG CATTTCAGCT GGCAAAGCAC TTATGGAATG GAACCTTTGA CTATGAGAAC 
TGTGACATCA ATCCCCTGGA TCCTGGAGAA TGCAATCCGC TTATCCCCAG AAAAGGACAG 
GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA CTTACTGAAC ACAATGAGAC GATTTTGTTC 
AAAGAGAAGT TTCTGGATTG GACGGAACTG AAGAGATCGA ATGAGAAGAA CCCCGGGGAA 
CTTGCCCAGC ACAAGGAAGA CCCCAGGACT GATGTCAAGG CATACGATGT GACACGGATG 
GTGTCCATGC CCCAGACGAC AGCAGGCACC ATCCTGGACG GAGTGAACGT CGGCCGTGGC 
TATGGCCTGG TGGAAGGACA CGACAGGAGG CAGTTTGAGA TCACCAGCGT TTCCGTGGAT 
GTCTGGCACA TCCTGGAATT CGACTATAGC AGGCTCCCCA AACAAAGCAT CGGGCAGTTC 
CATGAGGGGG ATGCCTATGT GGTCAAGTGG AAGTTCATGG TGAGCACGGC AGTGGGAAGT 
CGCCAGAAGG GAGAGCACTC GGTGAGGGCA GCCGGCAAAG AGAAGTGCGT CTACTTCTTC 
TGGCAAGGCC GGCACTCCAC CGTGAGTGAG AAGGGCACGT CGGCGCTGAT GACGGTGGAG 
CTGGACGAGG AAAGGGGGGC CCAGGTCCAG GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC 
CTGCAGTGTT TCCAGGGGGG GATGGTGGTG CACTCGGGGA GGCGGGAAGA GGAAGAAGAA 
AATGTGCAAA GTGAGTGGCG GCTGTACTGC GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT 
TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC CTGAGGTCCA GAACTTCCAT GGTGGTGCTT 
AACGTCAACA AGGCCCTCAT CTACCTGTGG CACGGATGCA AAGCCCAGGC CCACACGAAG 
GAGGTCGGAA GGACCGCTGC GAACAAGATC AAGGAACAAT GTCCCCTGGA AGCAGGACTG 
CATAGTAGCA GCAAAGTCAC AATACACGAG TGTGATGAAG GCTCCGAGCC ACTCGGATTC 
TGGGATGCCT TAGGAAGGAG AGACAGGAAA GCCTACGATT GCATGCTTCA AGATCCTGGA 
AGTTTTAACT TCGCGCCCCG CCTGTTCATC CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC 
ACAGAGTTTG TGTACCCTGC CCGAGCCCCC TCTGTGGTCA GTTCCATGCC CTTCCTGCAG 
GAAGATCTGT ACAGCGCGCC CCAGCCAGCA CTTTTCCTTG TTGACAATCA CCACGAGGTG 
TACCTCTGGC AAGGCTGGTG GCCCATCGAG AACAAGATCA CTGGTTCCGC CCGCATCCGC 
TGGGCCTCCG ACCGGAAGAG TGCGATGGAG ACTGTGCTCC AGTACTGCAA AGGAAAAAAT 
CTCAAGAAAC CAGCCCCCAA GTCTTACCTT ATCCACGCTG GTCTGGAGCC CCTGACATTC 
ACCAATATGT TTCCCAGCTG GGAGCACAGA GAGGACATCG CTGAGATCAC AGAGATGGAC 
ACGGAAGTTT CCAATCAGAT CACCCTCGTG GAAGACGTCT TAGCCAAGCT CTGTAAAACC 
ATTTACCCGC TGGCCGACCT CCTGGCCAGG CCACTCCCGG AGGGGGTCGA TCCTCTGAAG 
CTTGAGATCT ATCTCACCGA CGAAGACTTC GAGTTTGCAC TAGACATGAC GAGGGATGAA 
TACAACGCCC TGCCCGCCTG GAAGCAGGTG AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872066.1
CDS	1241..8140
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_017016577.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016577.1

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CTGAACCTAT CCCCGCTTCG 
TGTTCTGGGG GCACCCACCC TGTAATGGCG AGACTTCCTA GCCCCACTGT AGCTAGGAGC 
GCTGTGCAGC CTGCCAGATT GCAGGCCTCT GCTCACCAAA AGGCCTTAGC CAAGGACCAG 
ACAAATGAGG GCAAAGAGCT TGCTGAGCAA GGAGAACCTG ATTCCTCCAC TCTAAGCTTG 
GCCGAAAAGT TGGCCTTGTT TAACAAATTG TCCCAGCCAG TCTCAAAAGC GATTTCTACC 
CGGAACAGAA TAGACACGAG ACAGAGGAGA ATGAACGCTC GCTATCAAAC TCAGCCAGTC 
ACACTGGGAG AGGTGGAGCA GGTGCAGAGT GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG 
AACACATCAG TGTCTACCGT AGCATCCACG GTTGCTCCAA TGTATGCCGG AGATCTTCGC 
ACAAAGCCAC CTCTTGACCA CAATGCAAGT GCCACTGACT ATAAGTTTTC TTCTTCAATA 
GAAAATTCGG ACTCTCCAGT TAGAAGCATT CTGAAATCGC AAGCTTGGCA GCCTTTGGTA 
GAGGGTAGCG AGAACAAGGG AATGTTGAGA GAATATGGAG AGACAGAAAG CAAGAGAGCT 
TTGACAGGTC GAGACAGTGG GATGGAGAAG TATGGGTCCT TTGAGGAAGC AGAAGCATCC 
TACCCCATCC TGAACCGAGC CAGGGAAGGA GACAGCCATA AGGAATCTAA ATATGCTGTT 
CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC CCTCCCATCA CCCACCTCGG GGATGAACCG 
AAGGAATTTT CCATGGCTAA AATGAATGCA CAAGGAAACT TGGACTTGAG GGACAGGCTG 
CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT GTTATGAAAA GGAAGTTTTC ACTAAGAGCG 
GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG ACGGGGACAG CTGCTGGGAA AACTATTGCT 
CAAACCACAG CCCCCGTGTC CTGGAAGCCC CAGGATTCTT CGGAACAGCC ACAGGAGAAG 
CTCTGCAAGA ATCCATGTGC GATGTTTGCT GCTGGAGAGA TCAAAACGCC GACAGGGGAG 
GGCCTTCTTG ACTCACCCAG CAAAACCATG TCTATTAAAG AAAGATTGGC ACTGTTGAAG 
AAAAGCGGGG AGGAAGATTG GAGAAACAGA CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG 
CCGGCCAGCA GCCTGCACAC CCAGGAAGCA GGGCGGTCCC TCATCAAGAA GCGGGTCACA 
GAAAGTCGAG AGAGCCAAAT GACGATTGAG GAGAGGAAGC AGCTCATCAC TGTGAGAGAG 
GAGGCCTGGA AGACGAGAGG CAGAGGAGCG GCCAACGACT CGACCCAGTT CACTGTGGCT 
GGCAGGATGG TGAAGAAAGG TTTGGCGTCA CCTACTGCCA TAACCCCAGT AGCCTCACCC 
ATTTGCGGTA AAACAAGAGG CACCACACCC GTTTCCAAAC CCCTGGAAGA TATCGAAGCC 
AGACCAGATA TGCAGTTAGA ATCGGACCTG AAGTTGGACA GGCTGGAAAC CTTTCTAAGA 
AGGCTGAATA ACAAAGTTGG CGGGATGCAC GAAACGGTGC TCACTGTCAC CGGCAAATCT 
GTGAAGGAGG TGATGAAGCC AGATGATGAT GAAACCTTTG CCAAATTTTA CCGCAGCGTG 
GATTATAATA TGCCAAGAAG TCCTGTGGAG ATGGATGAGG ACTTCGATGT CATTTTCGAT 
CCTTATGCAC CCAAATTGAC GTCTTCCGTG GCCGAGCACA AGCGGGCAGT TAGGCCCAAG 
CGCCGGGTTC AGGCCTCCAA AAACCCCCTG AAAATGCTGG CGGCAAGAGA AGATCTCCTT 
CAGGAATACA CTGAGCAGAG ATTAAACGTT GCCTTCATGG AGTCAAAGCG GATGAAAGTA 
GAAAAGATGT CTTCCAACTC CAACTTCTCA GAAGTCACCC TGGCGGGTTT AGCCAGTAAA 
GAAAACTTCA GCAACGTCAG CCTGCGGAGC GTCAACCTGA CGGAACAGAA CTCTAACAAC 
AGCGCCGTGC CCTACAAGAG GCTGATGCTG TTGCAGATTA AAGGAAGAAG ACATGTGCAG 
ACCAGGCTGG TGGAACCTCG AGCTTCGGCG CTCAACAGTG GGGACTGCTT CCTCCTGCTC 
TCTCCCCACT GCTGCTTCCT GTGGGTAGGA GAGTTTGCAA ACGTCATAGA AAAGGCGAAG 
GCCTCAGAAC TTGCAACTTT AATTCAGACA AAGAGGGAAC TTGGTTGTAG AGCTACTTAT 
ATCCAAACCA TTGAAGAAGG AATTAATACA CACACTCATG CAGCCAAAGA CTTCTGGAAG 
CTTCTGGGTG GCCAAACCAG TTACCAATCT GCTGGAGACC CAAAAGAAGA TGAACTCTAT 
GAAGCAGCCA TAATAGAAAC TAACTGCATT TACCGTCTCA TGGATGACAA ACTTGTTCCT 
GATGACGACT ACTGGGGGAA AATTCCGAAG TGCTCCCTTC TGCAACCCAA AGAGGTACTG 
GTGTTTGATT TTGGTAGTGA AGTTTACGTA TGGCATGGGA AAGAAGTCAC ATTAGCACAA 
CGAAAAATAG CATTTCAGCT GGCAAAGCAC TTATGGAATG GAACCTTTGA CTATGAGAAC 
TGTGACATCA ATCCCCTGGA TCCTGGAGAA TGCAATCCGC TTATCCCCAG AAAAGGACAG 
GGGCGGCCCG ACTGGGCGAT ATTTGGGAGA CTTACTGAAC ACAATGAGAC GATTTTGTTC 
AAAGAGAAGT TTCTGGATTG GACGGAACTG AAGAGATCGA ATGAGAAGAA CCCCGGGGAA 
CTTGCCCAGC ACAAGGAAGA CCCCAGGACT GATGTCAAGG CATACGATGT GACACGGATG 
GTGTCCATGC CCCAGACGAC AGCAGGCACC ATCCTGGACG GAGTGAACGT CGGCCGTGGC 
TATGGCCTGG TGGAAGGACA CGACAGGAGG CAGTTTGAGA TCACCAGCGT TTCCGTGGAT 
GTCTGGCACA TCCTGGAATT CGACTATAGC AGGCTCCCCA AACAAAGCAT CGGGCAGTTC 
CATGAGGGGG ATGCCTATGT GGTCAAGTGG AAGTTCATGG TGAGCACGGC AGTGGGAAGT 
CGCCAGAAGG GAGAGCACTC GGTGAGGGCA GCCGGCAAAG AGAAGTGCGT CTACTTCTTC 
TGGCAAGGCC GGCACTCCAC CGTGAGTGAG AAGGGCACGT CGGCGCTGAT GACGGTGGAG 
CTGGACGAGG AAAGGGGGGC CCAGGTCCAG GTTCTCCAGG GAAAGGAGCC CCCCTGTTTC 
CTGCAGTGTT TCCAGGGGGG GATGGTGGTG CACTCGGGGA GGCGGGAAGA GGAAGAAGAA 
AATGTGCAAA GTGAGTGGCG GCTGTACTGC GTGCGTGGAG AGGTGCCCGT GGAAGGGAAT 
TTGCTGGAAG TGGCCTGTCA CTGTAGCAGC CTGAGGTCCA GAACTTCCAT GGTGGTGCTT 
AACGTCAACA AGGCCCTCAT CTACCTGTGG CACGGATGCA AAGCCCAGGC CCACACGAAG 
GAGGTCGGAA GGACCGCTGC GAACAAGATC AAGGAACAAT GTCCCCTGGA AGCAGGACTG 
CATAGTAGCA GCAAAGTCAC AATACACGAG TGTGATGAAG GCTCCGAGCC ACTCGGATTC 
TGGGATGCCT TAGGAAGGAG AGACAGGAAA GCCTACGATT GCATGCTTCA AGATCCTGGA 
AGTTTTAACT TCGCGCCCCG CCTGTTCATC CTCAGCAGCT CCTCTGGGGA TTTTGCAGCC 
ACAGAGTTTG TGTACCCTGC CCGAGCCCCC TCTGTGGTCA GTTCCATGCC CTTCCTGCAG 
GAAGATCTGT ACAGCGCGCC CCAGCCAGCA CTTTTCCTTG TTGACAATCA CCACGAGGTG 
TACCTCTGGC AAGGCTGGTG GCCCATCGAG AACAAGATCA CTGGTTCCGC CCGCATCCGC 
TGGGCCTCCG ACCGGAAGAG TGCGATGGAG ACTGTGCTCC AGTACTGCAA AGGAAAAAAT 
CTCAAGAAAC CAGCCCCCAA GTCTTACCTT ATCCACGCTG GTCTGGAGCC CCTGACATTC 
ACCAATATGT TTCCCAGCTG GGAGCACAGA GAGGACATCG CTGAGATCAC AGAGATGGAC 
ACGGAAGTTT CCAATCAGAT CACCCTCGTG GAAGACGTCT TAGCCAAGCT CTGTAAAACC 
ATTTACCCGC TGGCCGACCT CCTGGCCAGG CCACTCCCGG AGGGGGTCGA TCCTCTGAAG 
CTTGAGATCT ATCTCACCGA CGAAGACTTC GAGTTTGCAC TAGACATGAC GAGGGATGAA 
TACAACGCCC TGCCCGCCTG GAAGCAGGTG AACCTGAAGA AAGCAAAAGG CCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519632.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517934.2 Homo sapiens supervillin (SVIL), transcript variant X6, mRNA.

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CloneID	OHu91602
Clone ID Related Accession (Same CDS sequence)	XM_011519632.2
Accession Version	XM_011519632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTGTGCACT 
TCACATTCAG AAACGCCAAC TGTCGATGAT GAAGAAAAGG TGGATGAACG AGCCAAGCTG 
AGCGTCGCCG CCAAGAGGTT GCTTTTCAGG GAGATGGAAA AATCTTTTGA TGAACAAAAT 
GTTCCAAAGC GACGCTCAAG AAACACAGCT GTGGAGCAGA GGCTACGCCG TCTGCAGGAC 
AGGTCCCTCA CCCAGCCCAT CACCACTGAA GAGGTGGTCA TCGCAGCCAC TGAACCTATC 
CCCGCTTCGT GTTCTGGGGG CACCCACCCT GTAATGGCGA GACTTCCTAG CCCCACTGTA 
GCTAGGAGCG CTGTGCAGCC TGCCAGATTG CAGGCCTCTG CTCACCAAAA GGCCTTAGCC 
AAGGACCAGA CAAATGAGGG CAAAGAGCTT GCTGAGCAAG GAGAACCTGA TTCCTCCACT 
CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT AACAAATTGT CCCAGCCAGT CTCAAAAGCG 
ATTTCTACCC GGAACAGAAT AGACACGAGA CAGAGGAGAA TGAACGCTCG CTATCAAACT 
CAGCCAGTCA CACTGGGAGA GGTGGAGCAG GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA 
CCTGCCGTGA ACACATCAGT GTCTACCGTA GCATCCACGG TTGCTCCAAT GTATGCCGGA 
GATCTTCGCA CAAAGCCACC TCTTGACCAC AATGCAAGTG CCACTGACTA TAAGTTTTCT 
TCTTCAATAG AAAATTCGGA CTCTCCAGTT AGAAGCATTC TGAAATCGCA AGCTTGGCAG 
CCTTTGGTAG AGGGTAGCGA GAACAAGGGA ATGTTGAGAG AATATGGAGA GACAGAAAGC 
AAGAGAGCTT TGACAGGTCG AGACAGTGGG ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA 
GAAGCATCCT ACCCCATCCT GAACCGAGCC AGGGAAGGAG ACAGCCATAA GGAATCTAAA 
TATGCTGTTC CCAGAAGAGG AAGCCTGGAA CGGGCGAACC CTCCCATCAC CCACCTCGGG 
GATGAACCGA AGGAATTTTC CATGGCTAAA ATGAATGCAC AAGGAAACTT GGACTTGAGG 
GACAGGCTGC CCTTTGAAGA GAAGGTGGAG GTGGAGAATG TTATGAAAAG GAAGTTTTCA 
CTAAGAGCGG CAGAGTTCGG GGAGCCCACT TCCGAGCAGA CGGGGACAGC TGCTGGGAAA 
ACTATTGCTC AAACCACAGC CCCCGTGTCC TGGAAGCCCC AGGATTCTTC GGAACAGCCA 
CAGGAGAAGC TCTGCAAGAA TCCATGTGCG ATGTTTGCTG CTGGAGAGAT CAAAACGCCG 
ACAGGGGAGG GCCTTCTTGA CTCACCCAGC AAAACCATGT CTATTAAAGA AAGATTGGCA 
CTGTTGAAGA AAAGCGGGGA GGAAGATTGG AGAAACAGAC TCAGCAGGAG GCAGGAGGGC 
GGCAAGGCGC CGGCCAGCAG CCTGCACACC CAGGAAGCAG GGCGGTCCCT CATCAAGAAG 
GAAGAAGGAG GAGTTGCGGA TGATAGTGCC ATTTCTAATC TGCTTTGGGA ACCTGTATAT 
GCTTCTACTT ATTCTCCTGC TATTCCTGCT GCCCATAAAT ACCTGTCTTT TGTGTCGATT 
AATCAGCGGG TCACAGAAAG TCGAGAGAGC CAAATGACGA TTGAGGAGAG GAAGCAGCTC 
ATCACTGTGA GAGAGGAGGC CTGGAAGACG AGAGGCAGAG GAGCGGCCAA CGACTCGACC 
CAGTTCACTG TGGCTGGCAG GATGGTGAAG AAAGGTTTGG CGTCACCTAC TGCCATAACC 
CCAGTAGCCT CACCCATTTG CGGTAAAACA AGAGGCACCA CACCCGTTTC CAAACCCCTG 
GAAGATATCG AAGCCAGACC AGATATGCAG TTAGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGCGGGA TGCACGAAAC GGTGCTCACT 
GTCACCGGCA AATCTGTGAA GGAGGTGATG AAGCCAGATG ATGATGAAAC CTTTGCCAAA 
TTTTACCGCA GCGTGGATTA TAATATGCCA AGAAGTCCTG TGGAGATGGA TGAGGACTTC 
GATGTCATTT TCGATCCTTA TGCACCCAAA TTGACGTCTT CCGTGGCCGA GCACAAGCGG 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC TCCAAAAACC CCCTGAAAAT GCTGGCGGCA 
AGAGAAGATC TCCTTCAGGA ATACACTGAG CAGAGATTAA ACGTTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCTTCC AACTCCAACT TCTCAGAAGT CACCCTGGCG 
GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAACTCTA ACAACAGCGC CGTGCCCTAC AAGAGGCTGA TGCTGTTGCA GATTAAAGGA 
AGAAGACATG TGCAGACCAG GCTGGTGGAA CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCTCC CCACTGCTGC TTCCTGTGGG TAGGAGAGTT TGCAAACGTC 
ATAGAAAAGG CGAAGGCCTC AGAACTTGCA ACTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CTTATATCCA AACCATTGAA GAAGGAATTA ATACACACAC TCATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AATCTGCTGG AGACCCAAAA 
GAAGATGAAC TCTATGAAGC AGCCATAATA GAAACTAACT GCATTTACCG TCTCATGGAT 
GACAAACTTG TTCCTGATGA CGACTACTGG GGGAAAATTC CGAAGTGCTC CCTTCTGCAA 
CCCAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTTT ACGTATGGCA TGGGAAAGAA 
GTCACATTAG CACAACGAAA AATAGCATTT CAGCTGGCAA AGCACTTATG GAATGGAACC 
TTTGACTATG AGAACTGTGA CATCAATCCC CTGGATCCTG GAGAATGCAA TCCGCTTATC 
CCCAGAAAAG GACAGGGGCG GCCCGACTGG GCGATATTTG GGAGACTTAC TGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAGTTTCTG GATTGGACGG AACTGAAGAG ATCGAATGAG 
AAGAACCCCG GGGAACTTGC CCAGCACAAG GAAGACCCCA GGACTGATGT CAAGGCATAC 
GATGTGACAC GGATGGTGTC CATGCCCCAG ACGACAGCAG GCACCATCCT GGACGGAGTG 
AACGTCGGCC GTGGCTATGG CCTGGTGGAA GGACACGACA GGAGGCAGTT TGAGATCACC 
AGCGTTTCCG TGGATGTCTG GCACATCCTG GAATTCGACT ATAGCAGGCT CCCCAAACAA 
AGCATCGGGC AGTTCCATGA GGGGGATGCC TATGTGGTCA AGTGGAAGTT CATGGTGAGC 
ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG CACTCGGTGA GGGCAGCCGG CAAAGAGAAG 
TGCGTCTACT TCTTCTGGCA AGGCCGGCAC TCCACCGTGA GTGAGAAGGG CACGTCGGCG 
CTGATGACGG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG 
GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG GGGGGGATGG TGGTGCACTC GGGGAGGCGG 
GAAGAGGAAG AAGAAAATGT GCAAAGTGAG TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG 
CCCGTGGAAG GGAATTTGCT GGAAGTGGCC TGTCACTGTA GCAGCCTGAG GTCCAGAACT 
TCCATGGTGG TGCTTAACGT CAACAAGGCC CTCATCTACC TGTGGCACGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGAAGGACC GCTGCGAACA AGATCAAGGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTCACAATAC ACGAGTGTGA TGAAGGCTCC 
GAGCCACTCG GATTCTGGGA TGCCTTAGGA AGGAGAGACA GGAAAGCCTA CGATTGCATG 
CTTCAAGATC CTGGAAGTTT TAACTTCGCG CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGGGATTTTG CAGCCACAGA GTTTGTGTAC CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC 
ATGCCCTTCC TGCAGGAAGA TCTGTACAGC GCGCCCCAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACTGGT 
TCCGCCCGCA TCCGCTGGGC CTCCGACCGG AAGAGTGCGA TGGAGACTGT GCTCCAGTAC 
TGCAAAGGAA AAAATCTCAA GAAACCAGCC CCCAAGTCTT ACCTTATCCA CGCTGGTCTG 
GAGCCCCTGA CATTCACCAA TATGTTTCCC AGCTGGGAGC ACAGAGAGGA CATCGCTGAG 
ATCACAGAGA TGGACACGGA AGTTTCCAAT CAGATCACCC TCGTGGAAGA CGTCTTAGCC 
AAGCTCTGTA AAACCATTTA CCCGCTGGCC GACCTCCTGG CCAGGCCACT CCCGGAGGGG 
GTCGATCCTC TGAAGCTTGA GATCTATCTC ACCGACGAAG ACTTCGAGTT TGCACTAGAC 
ATGACGAGGG ATGAATACAA CGCCCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517934.2
CDS	1241..8095
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519632.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519632.2

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTGTGCACT 
TCACATTCAG AAACGCCAAC TGTCGATGAT GAAGAAAAGG TGGATGAACG AGCCAAGCTG 
AGCGTCGCCG CCAAGAGGTT GCTTTTCAGG GAGATGGAAA AATCTTTTGA TGAACAAAAT 
GTTCCAAAGC GACGCTCAAG AAACACAGCT GTGGAGCAGA GGCTACGCCG TCTGCAGGAC 
AGGTCCCTCA CCCAGCCCAT CACCACTGAA GAGGTGGTCA TCGCAGCCAC TGAACCTATC 
CCCGCTTCGT GTTCTGGGGG CACCCACCCT GTAATGGCGA GACTTCCTAG CCCCACTGTA 
GCTAGGAGCG CTGTGCAGCC TGCCAGATTG CAGGCCTCTG CTCACCAAAA GGCCTTAGCC 
AAGGACCAGA CAAATGAGGG CAAAGAGCTT GCTGAGCAAG GAGAACCTGA TTCCTCCACT 
CTAAGCTTGG CCGAAAAGTT GGCCTTGTTT AACAAATTGT CCCAGCCAGT CTCAAAAGCG 
ATTTCTACCC GGAACAGAAT AGACACGAGA CAGAGGAGAA TGAACGCTCG CTATCAAACT 
CAGCCAGTCA CACTGGGAGA GGTGGAGCAG GTGCAGAGTG GAAAGCTCAT TCCTTTCTCA 
CCTGCCGTGA ACACATCAGT GTCTACCGTA GCATCCACGG TTGCTCCAAT GTATGCCGGA 
GATCTTCGCA CAAAGCCACC TCTTGACCAC AATGCAAGTG CCACTGACTA TAAGTTTTCT 
TCTTCAATAG AAAATTCGGA CTCTCCAGTT AGAAGCATTC TGAAATCGCA AGCTTGGCAG 
CCTTTGGTAG AGGGTAGCGA GAACAAGGGA ATGTTGAGAG AATATGGAGA GACAGAAAGC 
AAGAGAGCTT TGACAGGTCG AGACAGTGGG ATGGAGAAGT ATGGGTCCTT TGAGGAAGCA 
GAAGCATCCT ACCCCATCCT GAACCGAGCC AGGGAAGGAG ACAGCCATAA GGAATCTAAA 
TATGCTGTTC CCAGAAGAGG AAGCCTGGAA CGGGCGAACC CTCCCATCAC CCACCTCGGG 
GATGAACCGA AGGAATTTTC CATGGCTAAA ATGAATGCAC AAGGAAACTT GGACTTGAGG 
GACAGGCTGC CCTTTGAAGA GAAGGTGGAG GTGGAGAATG TTATGAAAAG GAAGTTTTCA 
CTAAGAGCGG CAGAGTTCGG GGAGCCCACT TCCGAGCAGA CGGGGACAGC TGCTGGGAAA 
ACTATTGCTC AAACCACAGC CCCCGTGTCC TGGAAGCCCC AGGATTCTTC GGAACAGCCA 
CAGGAGAAGC TCTGCAAGAA TCCATGTGCG ATGTTTGCTG CTGGAGAGAT CAAAACGCCG 
ACAGGGGAGG GCCTTCTTGA CTCACCCAGC AAAACCATGT CTATTAAAGA AAGATTGGCA 
CTGTTGAAGA AAAGCGGGGA GGAAGATTGG AGAAACAGAC TCAGCAGGAG GCAGGAGGGC 
GGCAAGGCGC CGGCCAGCAG CCTGCACACC CAGGAAGCAG GGCGGTCCCT CATCAAGAAG 
GAAGAAGGAG GAGTTGCGGA TGATAGTGCC ATTTCTAATC TGCTTTGGGA ACCTGTATAT 
GCTTCTACTT ATTCTCCTGC TATTCCTGCT GCCCATAAAT ACCTGTCTTT TGTGTCGATT 
AATCAGCGGG TCACAGAAAG TCGAGAGAGC CAAATGACGA TTGAGGAGAG GAAGCAGCTC 
ATCACTGTGA GAGAGGAGGC CTGGAAGACG AGAGGCAGAG GAGCGGCCAA CGACTCGACC 
CAGTTCACTG TGGCTGGCAG GATGGTGAAG AAAGGTTTGG CGTCACCTAC TGCCATAACC 
CCAGTAGCCT CACCCATTTG CGGTAAAACA AGAGGCACCA CACCCGTTTC CAAACCCCTG 
GAAGATATCG AAGCCAGACC AGATATGCAG TTAGAATCGG ACCTGAAGTT GGACAGGCTG 
GAAACCTTTC TAAGAAGGCT GAATAACAAA GTTGGCGGGA TGCACGAAAC GGTGCTCACT 
GTCACCGGCA AATCTGTGAA GGAGGTGATG AAGCCAGATG ATGATGAAAC CTTTGCCAAA 
TTTTACCGCA GCGTGGATTA TAATATGCCA AGAAGTCCTG TGGAGATGGA TGAGGACTTC 
GATGTCATTT TCGATCCTTA TGCACCCAAA TTGACGTCTT CCGTGGCCGA GCACAAGCGG 
GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC TCCAAAAACC CCCTGAAAAT GCTGGCGGCA 
AGAGAAGATC TCCTTCAGGA ATACACTGAG CAGAGATTAA ACGTTGCCTT CATGGAGTCA 
AAGCGGATGA AAGTAGAAAA GATGTCTTCC AACTCCAACT TCTCAGAAGT CACCCTGGCG 
GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAACTCTA ACAACAGCGC CGTGCCCTAC AAGAGGCTGA TGCTGTTGCA GATTAAAGGA 
AGAAGACATG TGCAGACCAG GCTGGTGGAA CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC 
TGCTTCCTCC TGCTCTCTCC CCACTGCTGC TTCCTGTGGG TAGGAGAGTT TGCAAACGTC 
ATAGAAAAGG CGAAGGCCTC AGAACTTGCA ACTTTAATTC AGACAAAGAG GGAACTTGGT 
TGTAGAGCTA CTTATATCCA AACCATTGAA GAAGGAATTA ATACACACAC TCATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGTGGCCAA ACCAGTTACC AATCTGCTGG AGACCCAAAA 
GAAGATGAAC TCTATGAAGC AGCCATAATA GAAACTAACT GCATTTACCG TCTCATGGAT 
GACAAACTTG TTCCTGATGA CGACTACTGG GGGAAAATTC CGAAGTGCTC CCTTCTGCAA 
CCCAAAGAGG TACTGGTGTT TGATTTTGGT AGTGAAGTTT ACGTATGGCA TGGGAAAGAA 
GTCACATTAG CACAACGAAA AATAGCATTT CAGCTGGCAA AGCACTTATG GAATGGAACC 
TTTGACTATG AGAACTGTGA CATCAATCCC CTGGATCCTG GAGAATGCAA TCCGCTTATC 
CCCAGAAAAG GACAGGGGCG GCCCGACTGG GCGATATTTG GGAGACTTAC TGAACACAAT 
GAGACGATTT TGTTCAAAGA GAAGTTTCTG GATTGGACGG AACTGAAGAG ATCGAATGAG 
AAGAACCCCG GGGAACTTGC CCAGCACAAG GAAGACCCCA GGACTGATGT CAAGGCATAC 
GATGTGACAC GGATGGTGTC CATGCCCCAG ACGACAGCAG GCACCATCCT GGACGGAGTG 
AACGTCGGCC GTGGCTATGG CCTGGTGGAA GGACACGACA GGAGGCAGTT TGAGATCACC 
AGCGTTTCCG TGGATGTCTG GCACATCCTG GAATTCGACT ATAGCAGGCT CCCCAAACAA 
AGCATCGGGC AGTTCCATGA GGGGGATGCC TATGTGGTCA AGTGGAAGTT CATGGTGAGC 
ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG CACTCGGTGA GGGCAGCCGG CAAAGAGAAG 
TGCGTCTACT TCTTCTGGCA AGGCCGGCAC TCCACCGTGA GTGAGAAGGG CACGTCGGCG 
CTGATGACGG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG 
GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG GGGGGGATGG TGGTGCACTC GGGGAGGCGG 
GAAGAGGAAG AAGAAAATGT GCAAAGTGAG TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG 
CCCGTGGAAG GGAATTTGCT GGAAGTGGCC TGTCACTGTA GCAGCCTGAG GTCCAGAACT 
TCCATGGTGG TGCTTAACGT CAACAAGGCC CTCATCTACC TGTGGCACGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGAAGGACC GCTGCGAACA AGATCAAGGA ACAATGTCCC 
CTGGAAGCAG GACTGCATAG TAGCAGCAAA GTCACAATAC ACGAGTGTGA TGAAGGCTCC 
GAGCCACTCG GATTCTGGGA TGCCTTAGGA AGGAGAGACA GGAAAGCCTA CGATTGCATG 
CTTCAAGATC CTGGAAGTTT TAACTTCGCG CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGGGATTTTG CAGCCACAGA GTTTGTGTAC CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC 
ATGCCCTTCC TGCAGGAAGA TCTGTACAGC GCGCCCCAGC CAGCACTTTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACTGGT 
TCCGCCCGCA TCCGCTGGGC CTCCGACCGG AAGAGTGCGA TGGAGACTGT GCTCCAGTAC 
TGCAAAGGAA AAAATCTCAA GAAACCAGCC CCCAAGTCTT ACCTTATCCA CGCTGGTCTG 
GAGCCCCTGA CATTCACCAA TATGTTTCCC AGCTGGGAGC ACAGAGAGGA CATCGCTGAG 
ATCACAGAGA TGGACACGGA AGTTTCCAAT CAGATCACCC TCGTGGAAGA CGTCTTAGCC 
AAGCTCTGTA AAACCATTTA CCCGCTGGCC GACCTCCTGG CCAGGCCACT CCCGGAGGGG 
GTCGATCCTC TGAAGCTTGA GATCTATCTC ACCGACGAAG ACTTCGAGTT TGCACTAGAC 
ATGACGAGGG ATGAATACAA CGCCCTGCCC GCCTGGAAGC AGGTGAACCT GAAGAAAGCA 
AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519633.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517935.2 Homo sapiens supervillin (SVIL), transcript variant X7, mRNA.

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CloneID	OHu91603
Clone ID Related Accession (Same CDS sequence)	XM_011519633.2
Accession Version	XM_011519633.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519633.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CATTGCAGGC CTCTGCTCAC 
CAAAAGGCCT TAGCCAAGGA CCAGACAAAT GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA 
CCTGATTCCT CCACTCTAAG CTTGGCCGAA AAGTTGGCCT TGTTTAACAA ATTGTCCCAG 
CCAGTCTCAA AAGCGATTTC TACCCGGAAC AGAATAGACA CGAGACAGAG GAGAATGAAC 
GCTCGCTATC AAACTCAGCC AGTCACACTG GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG 
CTCATTCCTT TCTCACCTGC CGTGAACACA TCAGTGTCTA CCGTAGCATC CACGGTTGCT 
CCAATGTATG CCGGAGATCT TCGCACAAAG CCACCTCTTG ACCACAATGC AAGTGCCACT 
GACTATAAGT TTTCTTCTTC AATAGAAAAT TCGGACTCTC CAGTTAGAAG CATTCTGAAA 
TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT AGCGAGAACA AGGGAATGTT GAGAGAATAT 
GGAGAGACAG AAAGCAAGAG AGCTTTGACA GGTCGAGACA GTGGGATGGA GAAGTATGGG 
TCCTTTGAGG AAGCAGAAGC ATCCTACCCC ATCCTGAACC GAGCCAGGGA AGGAGACAGC 
CATAAGGAAT CTAAATATGC TGTTCCCAGA AGAGGAAGCC TGGAACGGGC GAACCCTCCC 
ATCACCCACC TCGGGGATGA ACCGAAGGAA TTTTCCATGG CTAAAATGAA TGCACAAGGA 
AACTTGGACT TGAGGGACAG GCTGCCCTTT GAAGAGAAGG TGGAGGTGGA GAATGTTATG 
AAAAGGAAGT TTTCACTAAG AGCGGCAGAG TTCGGGGAGC CCACTTCCGA GCAGACGGGG 
ACAGCTGCTG GGAAAACTAT TGCTCAAACC ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT 
TCTTCGGAAC AGCCACAGGA GAAGCTCTGC AAGAATCCAT GTGCGATGTT TGCTGCTGGA 
GAGATCAAAA CGCCGACAGG GGAGGGCCTT CTTGACTCAC CCAGCAAAAC CATGTCTATT 
AAAGAAAGAT TGGCACTGTT GAAGAAAAGC GGGGAGGAAG ATTGGAGAAA CAGACTCAGC 
AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC AGCAGCCTGC ACACCCAGGA AGCAGGGCGG 
TCCCTCATCA AGAAGCGGGT CACAGAAAGT CGAGAGAGCC AAATGACGAT TGAGGAGAGG 
AAGCAGCTCA TCACTGTGAG AGAGGAGGCC TGGAAGACGA GAGGCAGAGG AGCGGCCAAC 
GACTCGACCC AGTTCACTGT GGCTGGCAGG ATGGTGAAGA AAGGTTTGGC GTCACCTACT 
GCCATAACCC CAGTAGCCTC ACCCATTTGC GGTAAAACAA GAGGCACCAC ACCCGTTTCC 
AAACCCCTGG AAGATATCGA AGCCAGACCA GATATGCAGT TAGAATCGGA CCTGAAGTTG 
GACAGGCTGG AAACCTTTCT AAGAAGGCTG AATAACAAAG TTGGCGGGAT GCACGAAACG 
GTGCTCACTG TCACCGGCAA ATCTGTGAAG GAGGTGATGA AGCCAGATGA TGATGAAACC 
TTTGCCAAAT TTTACCGCAG CGTGGATTAT AATATGCCAA GAAGTCCTGT GGAGATGGAT 
GAGGACTTCG ATGTCATTTT CGATCCTTAT GCACCCAAAT TGACGTCTTC CGTGGCCGAG 
CACAAGCGGG CAGTTAGGCC CAAGCGCCGG GTTCAGGCCT CCAAAAACCC CCTGAAAATG 
CTGGCGGCAA GAGAAGATCT CCTTCAGGAA TACACTGAGC AGAGATTAAA CGTTGCCTTC 
ATGGAGTCAA AGCGGATGAA AGTAGAAAAG ATGTCTTCCA ACTCCAACTT CTCAGAAGTC 
ACCCTGGCGG GTTTAGCCAG TAAAGAAAAC TTCAGCAACG TCAGCCTGCG GAGCGTCAAC 
CTGACGGAAC AGAACTCTAA CAACAGCGCC GTGCCCTACA AGAGGCTGAT GCTGTTGCAG 
ATTAAAGGAA GAAGACATGT GCAGACCAGG CTGGTGGAAC CTCGAGCTTC GGCGCTCAAC 
AGTGGGGACT GCTTCCTCCT GCTCTCTCCC CACTGCTGCT TCCTGTGGGT AGGAGAGTTT 
GCAAACGTCA TAGAAAAGGC GAAGGCCTCA GAACTTGCAA CTTTAATTCA GACAAAGAGG 
GAACTTGGTT GTAGAGCTAC TTATATCCAA ACCATTGAAG AAGGAATTAA TACACACACT 
CATGCAGCCA AAGACTTCTG GAAGCTTCTG GGTGGCCAAA CCAGTTACCA ATCTGCTGGA 
GACCCAAAAG AAGATGAACT CTATGAAGCA GCCATAATAG AAACTAACTG CATTTACCGT 
CTCATGGATG ACAAACTTGT TCCTGATGAC GACTACTGGG GGAAAATTCC GAAGTGCTCC 
CTTCTGCAAC CCAAAGAGGT ACTGGTGTTT GATTTTGGTA GTGAAGTTTA CGTATGGCAT 
GGGAAAGAAG TCACATTAGC ACAACGAAAA ATAGCATTTC AGCTGGCAAA GCACTTATGG 
AATGGAACCT TTGACTATGA GAACTGTGAC ATCAATCCCC TGGATCCTGG AGAATGCAAT 
CCGCTTATCC CCAGAAAAGG ACAGGGGCGG CCCGACTGGG CGATATTTGG GAGACTTACT 
GAACACAATG AGACGATTTT GTTCAAAGAG AAGTTTCTGG ATTGGACGGA ACTGAAGAGA 
TCGAATGAGA AGAACCCCGG GGAACTTGCC CAGCACAAGG AAGACCCCAG GACTGATGTC 
AAGGCATACG ATGTGACACG GATGGTGTCC ATGCCCCAGA CGACAGCAGG CACCATCCTG 
GACGGAGTGA ACGTCGGCCG TGGCTATGGC CTGGTGGAAG GACACGACAG GAGGCAGTTT 
GAGATCACCA GCGTTTCCGT GGATGTCTGG CACATCCTGG AATTCGACTA TAGCAGGCTC 
CCCAAACAAA GCATCGGGCA GTTCCATGAG GGGGATGCCT ATGTGGTCAA GTGGAAGTTC 
ATGGTGAGCA CGGCAGTGGG AAGTCGCCAG AAGGGAGAGC ACTCGGTGAG GGCAGCCGGC 
AAAGAGAAGT GCGTCTACTT CTTCTGGCAA GGCCGGCACT CCACCGTGAG TGAGAAGGGC 
ACGTCGGCGC TGATGACGGT GGAGCTGGAC GAGGAAAGGG GGGCCCAGGT CCAGGTTCTC 
CAGGGAAAGG AGCCCCCCTG TTTCCTGCAG TGTTTCCAGG GGGGGATGGT GGTGCACTCG 
GGGAGGCGGG AAGAGGAAGA AGAAAATGTG CAAAGTGAGT GGCGGCTGTA CTGCGTGCGT 
GGAGAGGTGC CCGTGGAAGG GAATTTGCTG GAAGTGGCCT GTCACTGTAG CAGCCTGAGG 
TCCAGAACTT CCATGGTGGT GCTTAACGTC AACAAGGCCC TCATCTACCT GTGGCACGGA 
TGCAAAGCCC AGGCCCACAC GAAGGAGGTC GGAAGGACCG CTGCGAACAA GATCAAGGAA 
CAATGTCCCC TGGAAGCAGG ACTGCATAGT AGCAGCAAAG TCACAATACA CGAGTGTGAT 
GAAGGCTCCG AGCCACTCGG ATTCTGGGAT GCCTTAGGAA GGAGAGACAG GAAAGCCTAC 
GATTGCATGC TTCAAGATCC TGGAAGTTTT AACTTCGCGC CCCGCCTGTT CATCCTCAGC 
AGCTCCTCTG GGGATTTTGC AGCCACAGAG TTTGTGTACC CTGCCCGAGC CCCCTCTGTG 
GTCAGTTCCA TGCCCTTCCT GCAGGAAGAT CTGTACAGCG CGCCCCAGCC AGCACTTTTC 
CTTGTTGACA ATCACCACGA GGTGTACCTC TGGCAAGGCT GGTGGCCCAT CGAGAACAAG 
ATCACTGGTT CCGCCCGCAT CCGCTGGGCC TCCGACCGGA AGAGTGCGAT GGAGACTGTG 
CTCCAGTACT GCAAAGGAAA AAATCTCAAG AAACCAGCCC CCAAGTCTTA CCTTATCCAC 
GCTGGTCTGG AGCCCCTGAC ATTCACCAAT ATGTTTCCCA GCTGGGAGCA CAGAGAGGAC 
ATCGCTGAGA TCACAGAGAT GGACACGGAA GTTTCCAATC AGATCACCCT CGTGGAAGAC 
GTCTTAGCCA AGCTCTGTAA AACCATTTAC CCGCTGGCCG ACCTCCTGGC CAGGCCACTC 
CCGGAGGGGG TCGATCCTCT GAAGCTTGAG ATCTATCTCA CCGACGAAGA CTTCGAGTTT 
GCACTAGACA TGACGAGGGA TGAATACAAC GCCCTGCCCG CCTGGAAGCA GGTGAACCTG 
AAGAAAGCAA AAGGCCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517935.2
CDS	1241..8044
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519633.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519633.2

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGAGTTAAA GTTAGAGAAA AATTGGTGAA AGAGGAAAGT 
GCTCGAAACA GCCCTGAACT CGCCTCAGAG TCCGTAACTC AGAGGAGACA CCAGCCAGCG 
CCAGTCCATT ACGTGTCATT TCAGTCTGAG CACTCAGCCT TTGATAGGGT CCCCAGCAAG 
GCAGCAGGCT CTACACGACA GCCAATCCGT GGCTATGTCC AACCCGCAGA TACCGGTCAC 
ACCGCCAAGC TAGTGACGCC AGAAACCCCA GAAAATGCAT CTGAGTGTAG CTGGGTAGCA 
TCAGCCACCC AGAATGTCCC CAAACCTCCC AGCTTGACGG TTCTAGAAGG TGACGGAAGG 
GATAGCCCAG TTCTCCATGT CTGCGAGTCA AAAGCAGAAG AAGAAGAAGG GGAAGGAGAA 
GGAGAAGAAA AAGAAGAAGA TGTGTGCTTC ACTGAAGCTC TCGAGCAAAG CAAGAAAACC 
CTACTGGCTT TGGAGGGTGA TGGGCTAGTG AGAAGCCCAG AAGATCCCTC TAGAAATGAG 
GACTTTGGTA AGCCTGCTGT GAGCACAGTC ACCTTAGAGC ATCAGAAGGA ACTGGAAAAC 
GTGGCACAAC CCCCTCAAGC TCCGCACCAG CCCACTGAGA GGACAGGCAG GAGCGAGATG 
GTTCTCTACA TTCAAAGTGA GCCTGTGTCC CAAGACGCCA AACCAACTGG TCACAACAGG 
GAAGCCTCGA AAAAGCGCAA GGTCCGTACC CGCTCTCTGT CAGATTTCAC AGGCCCCCCT 
CAGCTCCAGG CCTTGAAGTA TAAGGACCCA GCTTCCAGGA GAGAGCTGGA GCTGCCCAGC 
TCCAAGACCG AAGGGCCTTA TGGGGAGATC AGCATGCTGG ACACAAAAGT CTCTGTCGCC 
CAGCTCCGAA GTGCGTTCCT GGCATCTGCC AACGCCTGCA GGAGACCTGA ACTCAAATCA 
CGGGTGGAGA GGTCGGCTGA AGGACCTGGC TTGCCCACCG GTGTGGAACG GGAGAGAGGG 
TCCCGGAAAC CAAGACGCTA TTTTTCTCCT GGTGAAAGTA GAAAAACTTC CGAGAGATTT 
AGAACCCAAC CTATAACTTC AGCAGAACGA AAGGAATCGG ATAGGTGCAC TTCACATTCA 
GAAACGCCAA CTGTCGATGA TGAAGAAAAG GTGGATGAAC GAGCCAAGCT GAGCGTCGCC 
GCCAAGAGGT TGCTTTTCAG GGAGATGGAA AAATCTTTTG ATGAACAAAA TGTTCCAAAG 
CGACGCTCAA GAAACACAGC TGTGGAGCAG AGGCTACGCC GTCTGCAGGA CAGGTCCCTC 
ACCCAGCCCA TCACCACTGA AGAGGTGGTC ATCGCAGCCA CATTGCAGGC CTCTGCTCAC 
CAAAAGGCCT TAGCCAAGGA CCAGACAAAT GAGGGCAAAG AGCTTGCTGA GCAAGGAGAA 
CCTGATTCCT CCACTCTAAG CTTGGCCGAA AAGTTGGCCT TGTTTAACAA ATTGTCCCAG 
CCAGTCTCAA AAGCGATTTC TACCCGGAAC AGAATAGACA CGAGACAGAG GAGAATGAAC 
GCTCGCTATC AAACTCAGCC AGTCACACTG GGAGAGGTGG AGCAGGTGCA GAGTGGAAAG 
CTCATTCCTT TCTCACCTGC CGTGAACACA TCAGTGTCTA CCGTAGCATC CACGGTTGCT 
CCAATGTATG CCGGAGATCT TCGCACAAAG CCACCTCTTG ACCACAATGC AAGTGCCACT 
GACTATAAGT TTTCTTCTTC AATAGAAAAT TCGGACTCTC CAGTTAGAAG CATTCTGAAA 
TCGCAAGCTT GGCAGCCTTT GGTAGAGGGT AGCGAGAACA AGGGAATGTT GAGAGAATAT 
GGAGAGACAG AAAGCAAGAG AGCTTTGACA GGTCGAGACA GTGGGATGGA GAAGTATGGG 
TCCTTTGAGG AAGCAGAAGC ATCCTACCCC ATCCTGAACC GAGCCAGGGA AGGAGACAGC 
CATAAGGAAT CTAAATATGC TGTTCCCAGA AGAGGAAGCC TGGAACGGGC GAACCCTCCC 
ATCACCCACC TCGGGGATGA ACCGAAGGAA TTTTCCATGG CTAAAATGAA TGCACAAGGA 
AACTTGGACT TGAGGGACAG GCTGCCCTTT GAAGAGAAGG TGGAGGTGGA GAATGTTATG 
AAAAGGAAGT TTTCACTAAG AGCGGCAGAG TTCGGGGAGC CCACTTCCGA GCAGACGGGG 
ACAGCTGCTG GGAAAACTAT TGCTCAAACC ACAGCCCCCG TGTCCTGGAA GCCCCAGGAT 
TCTTCGGAAC AGCCACAGGA GAAGCTCTGC AAGAATCCAT GTGCGATGTT TGCTGCTGGA 
GAGATCAAAA CGCCGACAGG GGAGGGCCTT CTTGACTCAC CCAGCAAAAC CATGTCTATT 
AAAGAAAGAT TGGCACTGTT GAAGAAAAGC GGGGAGGAAG ATTGGAGAAA CAGACTCAGC 
AGGAGGCAGG AGGGCGGCAA GGCGCCGGCC AGCAGCCTGC ACACCCAGGA AGCAGGGCGG 
TCCCTCATCA AGAAGCGGGT CACAGAAAGT CGAGAGAGCC AAATGACGAT TGAGGAGAGG 
AAGCAGCTCA TCACTGTGAG AGAGGAGGCC TGGAAGACGA GAGGCAGAGG AGCGGCCAAC 
GACTCGACCC AGTTCACTGT GGCTGGCAGG ATGGTGAAGA AAGGTTTGGC GTCACCTACT 
GCCATAACCC CAGTAGCCTC ACCCATTTGC GGTAAAACAA GAGGCACCAC ACCCGTTTCC 
AAACCCCTGG AAGATATCGA AGCCAGACCA GATATGCAGT TAGAATCGGA CCTGAAGTTG 
GACAGGCTGG AAACCTTTCT AAGAAGGCTG AATAACAAAG TTGGCGGGAT GCACGAAACG 
GTGCTCACTG TCACCGGCAA ATCTGTGAAG GAGGTGATGA AGCCAGATGA TGATGAAACC 
TTTGCCAAAT TTTACCGCAG CGTGGATTAT AATATGCCAA GAAGTCCTGT GGAGATGGAT 
GAGGACTTCG ATGTCATTTT CGATCCTTAT GCACCCAAAT TGACGTCTTC CGTGGCCGAG 
CACAAGCGGG CAGTTAGGCC CAAGCGCCGG GTTCAGGCCT CCAAAAACCC CCTGAAAATG 
CTGGCGGCAA GAGAAGATCT CCTTCAGGAA TACACTGAGC AGAGATTAAA CGTTGCCTTC 
ATGGAGTCAA AGCGGATGAA AGTAGAAAAG ATGTCTTCCA ACTCCAACTT CTCAGAAGTC 
ACCCTGGCGG GTTTAGCCAG TAAAGAAAAC TTCAGCAACG TCAGCCTGCG GAGCGTCAAC 
CTGACGGAAC AGAACTCTAA CAACAGCGCC GTGCCCTACA AGAGGCTGAT GCTGTTGCAG 
ATTAAAGGAA GAAGACATGT GCAGACCAGG CTGGTGGAAC CTCGAGCTTC GGCGCTCAAC 
AGTGGGGACT GCTTCCTCCT GCTCTCTCCC CACTGCTGCT TCCTGTGGGT AGGAGAGTTT 
GCAAACGTCA TAGAAAAGGC GAAGGCCTCA GAACTTGCAA CTTTAATTCA GACAAAGAGG 
GAACTTGGTT GTAGAGCTAC TTATATCCAA ACCATTGAAG AAGGAATTAA TACACACACT 
CATGCAGCCA AAGACTTCTG GAAGCTTCTG GGTGGCCAAA CCAGTTACCA ATCTGCTGGA 
GACCCAAAAG AAGATGAACT CTATGAAGCA GCCATAATAG AAACTAACTG CATTTACCGT 
CTCATGGATG ACAAACTTGT TCCTGATGAC GACTACTGGG GGAAAATTCC GAAGTGCTCC 
CTTCTGCAAC CCAAAGAGGT ACTGGTGTTT GATTTTGGTA GTGAAGTTTA CGTATGGCAT 
GGGAAAGAAG TCACATTAGC ACAACGAAAA ATAGCATTTC AGCTGGCAAA GCACTTATGG 
AATGGAACCT TTGACTATGA GAACTGTGAC ATCAATCCCC TGGATCCTGG AGAATGCAAT 
CCGCTTATCC CCAGAAAAGG ACAGGGGCGG CCCGACTGGG CGATATTTGG GAGACTTACT 
GAACACAATG AGACGATTTT GTTCAAAGAG AAGTTTCTGG ATTGGACGGA ACTGAAGAGA 
TCGAATGAGA AGAACCCCGG GGAACTTGCC CAGCACAAGG AAGACCCCAG GACTGATGTC 
AAGGCATACG ATGTGACACG GATGGTGTCC ATGCCCCAGA CGACAGCAGG CACCATCCTG 
GACGGAGTGA ACGTCGGCCG TGGCTATGGC CTGGTGGAAG GACACGACAG GAGGCAGTTT 
GAGATCACCA GCGTTTCCGT GGATGTCTGG CACATCCTGG AATTCGACTA TAGCAGGCTC 
CCCAAACAAA GCATCGGGCA GTTCCATGAG GGGGATGCCT ATGTGGTCAA GTGGAAGTTC 
ATGGTGAGCA CGGCAGTGGG AAGTCGCCAG AAGGGAGAGC ACTCGGTGAG GGCAGCCGGC 
AAAGAGAAGT GCGTCTACTT CTTCTGGCAA GGCCGGCACT CCACCGTGAG TGAGAAGGGC 
ACGTCGGCGC TGATGACGGT GGAGCTGGAC GAGGAAAGGG GGGCCCAGGT CCAGGTTCTC 
CAGGGAAAGG AGCCCCCCTG TTTCCTGCAG TGTTTCCAGG GGGGGATGGT GGTGCACTCG 
GGGAGGCGGG AAGAGGAAGA AGAAAATGTG CAAAGTGAGT GGCGGCTGTA CTGCGTGCGT 
GGAGAGGTGC CCGTGGAAGG GAATTTGCTG GAAGTGGCCT GTCACTGTAG CAGCCTGAGG 
TCCAGAACTT CCATGGTGGT GCTTAACGTC AACAAGGCCC TCATCTACCT GTGGCACGGA 
TGCAAAGCCC AGGCCCACAC GAAGGAGGTC GGAAGGACCG CTGCGAACAA GATCAAGGAA 
CAATGTCCCC TGGAAGCAGG ACTGCATAGT AGCAGCAAAG TCACAATACA CGAGTGTGAT 
GAAGGCTCCG AGCCACTCGG ATTCTGGGAT GCCTTAGGAA GGAGAGACAG GAAAGCCTAC 
GATTGCATGC TTCAAGATCC TGGAAGTTTT AACTTCGCGC CCCGCCTGTT CATCCTCAGC 
AGCTCCTCTG GGGATTTTGC AGCCACAGAG TTTGTGTACC CTGCCCGAGC CCCCTCTGTG 
GTCAGTTCCA TGCCCTTCCT GCAGGAAGAT CTGTACAGCG CGCCCCAGCC AGCACTTTTC 
CTTGTTGACA ATCACCACGA GGTGTACCTC TGGCAAGGCT GGTGGCCCAT CGAGAACAAG 
ATCACTGGTT CCGCCCGCAT CCGCTGGGCC TCCGACCGGA AGAGTGCGAT GGAGACTGTG 
CTCCAGTACT GCAAAGGAAA AAATCTCAAG AAACCAGCCC CCAAGTCTTA CCTTATCCAC 
GCTGGTCTGG AGCCCCTGAC ATTCACCAAT ATGTTTCCCA GCTGGGAGCA CAGAGAGGAC 
ATCGCTGAGA TCACAGAGAT GGACACGGAA GTTTCCAATC AGATCACCCT CGTGGAAGAC 
GTCTTAGCCA AGCTCTGTAA AACCATTTAC CCGCTGGCCG ACCTCCTGGC CAGGCCACTC 
CCGGAGGGGG TCGATCCTCT GAAGCTTGAG ATCTATCTCA CCGACGAAGA CTTCGAGTTT 
GCACTAGACA TGACGAGGGA TGAATACAAC GCCCTGCCCG CCTGGAAGCA GGTGAACCTG 
AAGAAAGCAA AAGGCCTGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519636.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517938.2 Homo sapiens supervillin (SVIL), transcript variant X13, mRNA.

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CloneID	OHu91604
Clone ID Related Accession (Same CDS sequence)	XM_011519636.2
Accession Version	XM_011519636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGCAAATCA CGGGTGGAGA GGTCGGCTGA AGGACCTGGC 
TTGCCCACCG GTGTGGAACG GGAGAGAGGG TCCCGGAAAC CAAGACGCTA TTTTTCTCCT 
GGTGAAAGTA GAAAAACTTC CGAGAGATTT AGAACCCAAC CTATAACTTC AGCAGAACGA 
AAGGAATCGG ATAGGTGCAC TTCACATTCA GAAACGCCAA CTGTCGATGA TGAAGAAAAG 
GTGGATGAAC GAGCCAAGCT GAGCGTCGCC GCCAAGAGGT TGCTTTTCAG GGAGATGGAA 
AAATCTTTTG ATGAACAAAA TGTTCCAAAG CGACGCTCAA GAAACACAGC TGTGGAGCAG 
AGGCTACGCC GTCTGCAGGA CAGGTCCCTC ACCCAGCCCA TCACCACTGA AGAGGTGGTC 
ATCGCAGCCA CTGAACCTAT CCCCGCTTCG TGTTCTGGGG GCACCCACCC TGTAATGGCG 
AGACTTCCTA GCCCCACTGT AGCTAGGAGC GCTGTGCAGC CTGCCAGATT GCAGGCCTCT 
GCTCACCAAA AGGCCTTAGC CAAGGACCAG ACAAATGAGG GCAAAGAGCT TGCTGAGCAA 
GGAGAACCTG ATTCCTCCAC TCTAAGCTTG GCCGAAAAGT TGGCCTTGTT TAACAAATTG 
TCCCAGCCAG TCTCAAAAGC GATTTCTACC CGGAACAGAA TAGACACGAG ACAGAGGAGA 
ATGAACGCTC GCTATCAAAC TCAGCCAGTC ACACTGGGAG AGGTGGAGCA GGTGCAGAGT 
GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG AACACATCAG TGTCTACCGT AGCATCCACG 
GTTGCTCCAA TGTATGCCGG AGATCTTCGC ACAAAGCCAC CTCTTGACCA CAATGCAAGT 
GCCACTGACT ATAAGTTTTC TTCTTCAATA GAAAATTCGG ACTCTCCAGT TAGAAGCATT 
CTGAAATCGC AAGCTTGGCA GCCTTTGGTA GAGGGTAGCG AGAACAAGGG AATGTTGAGA 
GAATATGGAG AGACAGAAAG CAAGAGAGCT TTGACAGGTC GAGACAGTGG GATGGAGAAG 
TATGGGTCCT TTGAGGAAGC AGAAGCATCC TACCCCATCC TGAACCGAGC CAGGGAAGGA 
GACAGCCATA AGGAATCTAA ATATGCTGTT CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC 
CCTCCCATCA CCCACCTCGG GGATGAACCG AAGGAATTTT CCATGGCTAA AATGAATGCA 
CAAGGAAACT TGGACTTGAG GGACAGGCTG CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT 
GTTATGAAAA GGAAGTTTTC ACTAAGAGCG GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG 
ACGGGGACAG CTGCTGGGAA AACTATTGCT CAAACCACAG CCCCCGTGTC CTGGAAGCCC 
CAGGATTCTT CGGAACAGCC ACAGGAGAAG CTCTGCAAGA ATCCATGTGC GATGTTTGCT 
GCTGGAGAGA TCAAAACGCC GACAGGGGAG GGCCTTCTTG ACTCACCCAG CAAAACCATG 
TCTATTAAAG AAAGATTGGC ACTGTTGAAG AAAAGCGGGG AGGAAGATTG GAGAAACAGA 
CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG CCGGCCAGCA GCCTGCACAC CCAGGAAGCA 
GGGCGGTCCC TCATCAAGAA GGAAGAAGGA GGAGTTGCGG ATGATAGTGC CATTTCTAAT 
CTGCTTTGGG AACCTGTATA TGCTTCTACT TATTCTCCTG CTATTCCTGC TGCCCATAAA 
TACCTGTCTT TTGTGTCGAT TAATCAGCGG GTCACAGAAA GTCGAGAGAG CCAAATGACG 
ATTGAGGAGA GGAAGCAGCT CATCACTGTG AGAGAGGAGG CCTGGAAGAC GAGAGGCAGA 
GGAGCGGCCA ACGACTCGAC CCAGTTCACT GTGGCTGGCA GGATGGTGAA GAAAGGTTTG 
GCGTCACCTA CTGCCATAAC CCCAGTAGCC TCACCCATTT GCGGTAAAAC AAGAGGCACC 
ACACCCGTTT CCAAACCCCT GGAAGATATC GAAGCCAGAC CAGATATGCA GTTAGAATCG 
GACCTGAAGT TGGACAGGCT GGAAACCTTT CTAAGAAGGC TGAATAACAA AGTTGGCGGG 
ATGCACGAAA CGGTGCTCAC TGTCACCGGC AAATCTGTGA AGGAGGTGAT GAAGCCAGAT 
GATGATGAAA CCTTTGCCAA ATTTTACCGC AGCGTGGATT ATAATATGCC AAGAAGTCCT 
GTGGAGATGG ATGAGGACTT CGATGTCATT TTCGATCCTT ATGCACCCAA ATTGACGTCT 
TCCGTGGCCG AGCACAAGCG GGCAGTTAGG CCCAAGCGCC GGGTTCAGGC CTCCAAAAAC 
CCCCTGAAAA TGCTGGCGGC AAGAGAAGAT CTCCTTCAGG AATACACTGA GCAGAGATTA 
AACGTTGCCT TCATGGAGTC AAAGCGGATG AAAGTAGAAA AGATGTCTTC CAACTCCAAC 
TTCTCAGAAG TCACCCTGGC GGGTTTAGCC AGTAAAGAAA ACTTCAGCAA CGTCAGCCTG 
CGGAGCGTCA ACCTGACGGA ACAGAACTCT AACAACAGCG CCGTGCCCTA CAAGAGGCTG 
ATGCTGTTGC AGATTAAAGG AAGAAGACAT GTGCAGACCA GGCTGGTGGA ACCTCGAGCT 
TCGGCGCTCA ACAGTGGGGA CTGCTTCCTC CTGCTCTCTC CCCACTGCTG CTTCCTGTGG 
GTAGGAGAGT TTGCAAACGT CATAGAAAAG GCGAAGGCCT CAGAACTTGC AACTTTAATT 
CAGACAAAGA GGGAACTTGG TTGTAGAGCT ACTTATATCC AAACCATTGA AGAAGGAATT 
AATACACACA CTCATGCAGC CAAAGACTTC TGGAAGCTTC TGGGTGGCCA AACCAGTTAC 
CAATCTGCTG GAGACCCAAA AGAAGATGAA CTCTATGAAG CAGCCATAAT AGAAACTAAC 
TGCATTTACC GTCTCATGGA TGACAAACTT GTTCCTGATG ACGACTACTG GGGGAAAATT 
CCGAAGTGCT CCCTTCTGCA ACCCAAAGAG GTACTGGTGT TTGATTTTGG TAGTGAAGTT 
TACGTATGGC ATGGGAAAGA AGTCACATTA GCACAACGAA AAATAGCATT TCAGCTGGCA 
AAGCACTTAT GGAATGGAAC CTTTGACTAT GAGAACTGTG ACATCAATCC CCTGGATCCT 
GGAGAATGCA ATCCGCTTAT CCCCAGAAAA GGACAGGGGC GGCCCGACTG GGCGATATTT 
GGGAGACTTA CTGAACACAA TGAGACGATT TTGTTCAAAG AGAAGTTTCT GGATTGGACG 
GAACTGAAGA GATCGAATGA GAAGAACCCC GGGGAACTTG CCCAGCACAA GGAAGACCCC 
AGGACTGATG TCAAGGCATA CGATGTGACA CGGATGGTGT CCATGCCCCA GACGACAGCA 
GGCACCATCC TGGACGGAGT GAACGTCGGC CGTGGCTATG GCCTGGTGGA AGGACACGAC 
AGGAGGCAGT TTGAGATCAC CAGCGTTTCC GTGGATGTCT GGCACATCCT GGAATTCGAC 
TATAGCAGGC TCCCCAAACA AAGCATCGGG CAGTTCCATG AGGGGGATGC CTATGTGGTC 
AAGTGGAAGT TCATGGTGAG CACGGCAGTG GGAAGTCGCC AGAAGGGAGA GCACTCGGTG 
AGGGCAGCCG GCAAAGAGAA GTGCGTCTAC TTCTTCTGGC AAGGCCGGCA CTCCACCGTG 
AGTGAGAAGG GCACGTCGGC GCTGATGACG GTGGAGCTGG ACGAGGAAAG GGGGGCCCAG 
GTCCAGGTTC TCCAGGGAAA GGAGCCCCCC TGTTTCCTGC AGTGTTTCCA GGGGGGGATG 
GTGGTGCACT CGGGGAGGCG GGAAGAGGAA GAAGAAAATG TGCAAAGTGA GTGGCGGCTG 
TACTGCGTGC GTGGAGAGGT GCCCGTGGAA GGGAATTTGC TGGAAGTGGC CTGTCACTGT 
AGCAGCCTGA GGTCCAGAAC TTCCATGGTG GTGCTTAACG TCAACAAGGC CCTCATCTAC 
CTGTGGCACG GATGCAAAGC CCAGGCCCAC ACGAAGGAGG TCGGAAGGAC CGCTGCGAAC 
AAGATCAAGG AACAATGTCC CCTGGAAGCA GGACTGCATA GTAGCAGCAA AGTCACAATA 
CACGAGTGTG ATGAAGGCTC CGAGCCACTC GGATTCTGGG ATGCCTTAGG AAGGAGAGAC 
AGGAAAGCCT ACGATTGCAT GCTTCAAGAT CCTGGAAGTT TTAACTTCGC GCCCCGCCTG 
TTCATCCTCA GCAGCTCCTC TGGGGATTTT GCAGCCACAG AGTTTGTGTA CCCTGCCCGA 
GCCCCCTCTG TGGTCAGTTC CATGCCCTTC CTGCAGGAAG ATCTGTACAG CGCGCCCCAG 
CCAGCACTTT TCCTTGTTGA CAATCACCAC GAGGTGTACC TCTGGCAAGG CTGGTGGCCC 
ATCGAGAACA AGATCACTGG TTCCGCCCGC ATCCGCTGGG CCTCCGACCG GAAGAGTGCG 
ATGGAGACTG TGCTCCAGTA CTGCAAAGGA AAAAATCTCA AGAAACCAGC CCCCAAGTCT 
TACCTTATCC ACGCTGGTCT GGAGCCCCTG ACATTCACCA ATATGTTTCC CAGCTGGGAG 
CACAGAGAGG ACATCGCTGA GATCACAGAG ATGGACACGG AAGTTTCCAA TCAGATCACC 
CTCGTGGAAG ACGTCTTAGC CAAGCTCTGT AAAACCATTT ACCCGCTGGC CGACCTCCTG 
GCCAGGCCAC TCCCGGAGGG GGTCGATCCT CTGAAGCTTG AGATCTATCT CACCGACGAA 
GACTTCGAGT TTGCACTAGA CATGACGAGG GATGAATACA ACGCCCTGCC CGCCTGGAAG 
CAGGTGAACC TGAAGAAAGC AAAAGGCCTG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517938.2
CDS	1241..7336
Translation	MPVRCGDPGDMKGEEQKTDFNKKNLSFLGFPRQRKVSSFQKEFSLEDKEQLANHERGIDAQLLVALPKVAELRQIFEPKKKEFLEMKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSSKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519636.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519636.2

ORF Insert Sequence:

ATGCCTGTGA GATGTGGGGA TCCAGGTGAC ATGAAGGGAG AAGAACAGAA GACAGATTTC 
AACAAGAAAA ACTTAAGCTT CCTTGGATTC CCACGTCAAA GGAAAGTTTC AAGCTTTCAG 
AAGGAGTTCT CACTCGAAGA TAAAGAACAG CTCGCTAACC ACGAAAGAGG AATCGATGCT 
CAGCTTTTAG TTGCACTTCC TAAAGTTGCA GAATTAAGAC AAATCTTTGA ACCAAAGAAG 
AAAGAATTCT TAGAAATGAA AAGAAAAGAA AGAATTGCCA GGCGCCTGGA AGGGATTGAA 
AATGACACTC AGCCCATCCT CTTGCAGAGC TGCACAGGAT TGGTGACTCA CCGCCTGCTG 
GAGGAAGACA CCCCTCGATA CATGAGAGCC AGCGACCCTG CCAGCCCCCA CATCGGCCGA 
TCAAATGAAG AGGAGGAAAC TTCTGATTCT TCTCTAGAAA AGCAAACTCG ATCCAAATAC 
TGCACAGAAA CCTCCGGTGT CCACGGTGAC TCACCCTATG GTTCGGGTAC CATGGACACC 
CACAGTCTGG AGTCCAAAGC CGAAAGAATT GCAAGGTACA AAGCAGAAAG AAGGCGACAG 
CTGGCAGAGA AGTATGGGCT GACTCTGGAT CCCGAGGCCG ACTCCGAGTA TTTATCCCGC 
TATACCAAGT CCAGGAAGGA GCCTGATGCT GTCGAGAAGC GGGGAGGAAA AAGTGACAAA 
CAGGAAGAGT CAAGCAGAGA TGCTAGTTCT CTGTACCCCG GGACCGAGAC GATGGGGCTC 
AGGACCTGTG CCGGTGAATC CAAGGACTAT GCCCTCCATG TGGGTGACGG CTCTTCCGAC 
CCGGAGGTGC TGCTGAACAT AGAAAACCAA AGACGAGGTC AAGAGCTGAG TGCCACCCGG 
CAGGCCCATG ACCTGTCCCC AGCAGCCGAG AGTTCCTCGA CCTTCTCTTT CTCTGGGCGA 
GACTCCTCCT TCACTGAAGT GCCACGGTCC CCCAAGCACG CCCACAGCTC CTCCCTGCAG 
CAGGCAGCCT CCCGGAGCCC CTCCTTTGGT GACCCACAGC TATCCCCTGA GGCCCGACCC 
AGTACAGGGA AACCCAAACA TGAGTGGTTT CTCCAGAAAG ATTCCGAAGG GGACACACCT 
TCACTTATCA ACTGGCCTTC CAGCAAATCA CGGGTGGAGA GGTCGGCTGA AGGACCTGGC 
TTGCCCACCG GTGTGGAACG GGAGAGAGGG TCCCGGAAAC CAAGACGCTA TTTTTCTCCT 
GGTGAAAGTA GAAAAACTTC CGAGAGATTT AGAACCCAAC CTATAACTTC AGCAGAACGA 
AAGGAATCGG ATAGGTGCAC TTCACATTCA GAAACGCCAA CTGTCGATGA TGAAGAAAAG 
GTGGATGAAC GAGCCAAGCT GAGCGTCGCC GCCAAGAGGT TGCTTTTCAG GGAGATGGAA 
AAATCTTTTG ATGAACAAAA TGTTCCAAAG CGACGCTCAA GAAACACAGC TGTGGAGCAG 
AGGCTACGCC GTCTGCAGGA CAGGTCCCTC ACCCAGCCCA TCACCACTGA AGAGGTGGTC 
ATCGCAGCCA CTGAACCTAT CCCCGCTTCG TGTTCTGGGG GCACCCACCC TGTAATGGCG 
AGACTTCCTA GCCCCACTGT AGCTAGGAGC GCTGTGCAGC CTGCCAGATT GCAGGCCTCT 
GCTCACCAAA AGGCCTTAGC CAAGGACCAG ACAAATGAGG GCAAAGAGCT TGCTGAGCAA 
GGAGAACCTG ATTCCTCCAC TCTAAGCTTG GCCGAAAAGT TGGCCTTGTT TAACAAATTG 
TCCCAGCCAG TCTCAAAAGC GATTTCTACC CGGAACAGAA TAGACACGAG ACAGAGGAGA 
ATGAACGCTC GCTATCAAAC TCAGCCAGTC ACACTGGGAG AGGTGGAGCA GGTGCAGAGT 
GGAAAGCTCA TTCCTTTCTC ACCTGCCGTG AACACATCAG TGTCTACCGT AGCATCCACG 
GTTGCTCCAA TGTATGCCGG AGATCTTCGC ACAAAGCCAC CTCTTGACCA CAATGCAAGT 
GCCACTGACT ATAAGTTTTC TTCTTCAATA GAAAATTCGG ACTCTCCAGT TAGAAGCATT 
CTGAAATCGC AAGCTTGGCA GCCTTTGGTA GAGGGTAGCG AGAACAAGGG AATGTTGAGA 
GAATATGGAG AGACAGAAAG CAAGAGAGCT TTGACAGGTC GAGACAGTGG GATGGAGAAG 
TATGGGTCCT TTGAGGAAGC AGAAGCATCC TACCCCATCC TGAACCGAGC CAGGGAAGGA 
GACAGCCATA AGGAATCTAA ATATGCTGTT CCCAGAAGAG GAAGCCTGGA ACGGGCGAAC 
CCTCCCATCA CCCACCTCGG GGATGAACCG AAGGAATTTT CCATGGCTAA AATGAATGCA 
CAAGGAAACT TGGACTTGAG GGACAGGCTG CCCTTTGAAG AGAAGGTGGA GGTGGAGAAT 
GTTATGAAAA GGAAGTTTTC ACTAAGAGCG GCAGAGTTCG GGGAGCCCAC TTCCGAGCAG 
ACGGGGACAG CTGCTGGGAA AACTATTGCT CAAACCACAG CCCCCGTGTC CTGGAAGCCC 
CAGGATTCTT CGGAACAGCC ACAGGAGAAG CTCTGCAAGA ATCCATGTGC GATGTTTGCT 
GCTGGAGAGA TCAAAACGCC GACAGGGGAG GGCCTTCTTG ACTCACCCAG CAAAACCATG 
TCTATTAAAG AAAGATTGGC ACTGTTGAAG AAAAGCGGGG AGGAAGATTG GAGAAACAGA 
CTCAGCAGGA GGCAGGAGGG CGGCAAGGCG CCGGCCAGCA GCCTGCACAC CCAGGAAGCA 
GGGCGGTCCC TCATCAAGAA GGAAGAAGGA GGAGTTGCGG ATGATAGTGC CATTTCTAAT 
CTGCTTTGGG AACCTGTATA TGCTTCTACT TATTCTCCTG CTATTCCTGC TGCCCATAAA 
TACCTGTCTT TTGTGTCGAT TAATCAGCGG GTCACAGAAA GTCGAGAGAG CCAAATGACG 
ATTGAGGAGA GGAAGCAGCT CATCACTGTG AGAGAGGAGG CCTGGAAGAC GAGAGGCAGA 
GGAGCGGCCA ACGACTCGAC CCAGTTCACT GTGGCTGGCA GGATGGTGAA GAAAGGTTTG 
GCGTCACCTA CTGCCATAAC CCCAGTAGCC TCACCCATTT GCGGTAAAAC AAGAGGCACC 
ACACCCGTTT CCAAACCCCT GGAAGATATC GAAGCCAGAC CAGATATGCA GTTAGAATCG 
GACCTGAAGT TGGACAGGCT GGAAACCTTT CTAAGAAGGC TGAATAACAA AGTTGGCGGG 
ATGCACGAAA CGGTGCTCAC TGTCACCGGC AAATCTGTGA AGGAGGTGAT GAAGCCAGAT 
GATGATGAAA CCTTTGCCAA ATTTTACCGC AGCGTGGATT ATAATATGCC AAGAAGTCCT 
GTGGAGATGG ATGAGGACTT CGATGTCATT TTCGATCCTT ATGCACCCAA ATTGACGTCT 
TCCGTGGCCG AGCACAAGCG GGCAGTTAGG CCCAAGCGCC GGGTTCAGGC CTCCAAAAAC 
CCCCTGAAAA TGCTGGCGGC AAGAGAAGAT CTCCTTCAGG AATACACTGA GCAGAGATTA 
AACGTTGCCT TCATGGAGTC AAAGCGGATG AAAGTAGAAA AGATGTCTTC CAACTCCAAC 
TTCTCAGAAG TCACCCTGGC GGGTTTAGCC AGTAAAGAAA ACTTCAGCAA CGTCAGCCTG 
CGGAGCGTCA ACCTGACGGA ACAGAACTCT AACAACAGCG CCGTGCCCTA CAAGAGGCTG 
ATGCTGTTGC AGATTAAAGG AAGAAGACAT GTGCAGACCA GGCTGGTGGA ACCTCGAGCT 
TCGGCGCTCA ACAGTGGGGA CTGCTTCCTC CTGCTCTCTC CCCACTGCTG CTTCCTGTGG 
GTAGGAGAGT TTGCAAACGT CATAGAAAAG GCGAAGGCCT CAGAACTTGC AACTTTAATT 
CAGACAAAGA GGGAACTTGG TTGTAGAGCT ACTTATATCC AAACCATTGA AGAAGGAATT 
AATACACACA CTCATGCAGC CAAAGACTTC TGGAAGCTTC TGGGTGGCCA AACCAGTTAC 
CAATCTGCTG GAGACCCAAA AGAAGATGAA CTCTATGAAG CAGCCATAAT AGAAACTAAC 
TGCATTTACC GTCTCATGGA TGACAAACTT GTTCCTGATG ACGACTACTG GGGGAAAATT 
CCGAAGTGCT CCCTTCTGCA ACCCAAAGAG GTACTGGTGT TTGATTTTGG TAGTGAAGTT 
TACGTATGGC ATGGGAAAGA AGTCACATTA GCACAACGAA AAATAGCATT TCAGCTGGCA 
AAGCACTTAT GGAATGGAAC CTTTGACTAT GAGAACTGTG ACATCAATCC CCTGGATCCT 
GGAGAATGCA ATCCGCTTAT CCCCAGAAAA GGACAGGGGC GGCCCGACTG GGCGATATTT 
GGGAGACTTA CTGAACACAA TGAGACGATT TTGTTCAAAG AGAAGTTTCT GGATTGGACG 
GAACTGAAGA GATCGAATGA GAAGAACCCC GGGGAACTTG CCCAGCACAA GGAAGACCCC 
AGGACTGATG TCAAGGCATA CGATGTGACA CGGATGGTGT CCATGCCCCA GACGACAGCA 
GGCACCATCC TGGACGGAGT GAACGTCGGC CGTGGCTATG GCCTGGTGGA AGGACACGAC 
AGGAGGCAGT TTGAGATCAC CAGCGTTTCC GTGGATGTCT GGCACATCCT GGAATTCGAC 
TATAGCAGGC TCCCCAAACA AAGCATCGGG CAGTTCCATG AGGGGGATGC CTATGTGGTC 
AAGTGGAAGT TCATGGTGAG CACGGCAGTG GGAAGTCGCC AGAAGGGAGA GCACTCGGTG 
AGGGCAGCCG GCAAAGAGAA GTGCGTCTAC TTCTTCTGGC AAGGCCGGCA CTCCACCGTG 
AGTGAGAAGG GCACGTCGGC GCTGATGACG GTGGAGCTGG ACGAGGAAAG GGGGGCCCAG 
GTCCAGGTTC TCCAGGGAAA GGAGCCCCCC TGTTTCCTGC AGTGTTTCCA GGGGGGGATG 
GTGGTGCACT CGGGGAGGCG GGAAGAGGAA GAAGAAAATG TGCAAAGTGA GTGGCGGCTG 
TACTGCGTGC GTGGAGAGGT GCCCGTGGAA GGGAATTTGC TGGAAGTGGC CTGTCACTGT 
AGCAGCCTGA GGTCCAGAAC TTCCATGGTG GTGCTTAACG TCAACAAGGC CCTCATCTAC 
CTGTGGCACG GATGCAAAGC CCAGGCCCAC ACGAAGGAGG TCGGAAGGAC CGCTGCGAAC 
AAGATCAAGG AACAATGTCC CCTGGAAGCA GGACTGCATA GTAGCAGCAA AGTCACAATA 
CACGAGTGTG ATGAAGGCTC CGAGCCACTC GGATTCTGGG ATGCCTTAGG AAGGAGAGAC 
AGGAAAGCCT ACGATTGCAT GCTTCAAGAT CCTGGAAGTT TTAACTTCGC GCCCCGCCTG 
TTCATCCTCA GCAGCTCCTC TGGGGATTTT GCAGCCACAG AGTTTGTGTA CCCTGCCCGA 
GCCCCCTCTG TGGTCAGTTC CATGCCCTTC CTGCAGGAAG ATCTGTACAG CGCGCCCCAG 
CCAGCACTTT TCCTTGTTGA CAATCACCAC GAGGTGTACC TCTGGCAAGG CTGGTGGCCC 
ATCGAGAACA AGATCACTGG TTCCGCCCGC ATCCGCTGGG CCTCCGACCG GAAGAGTGCG 
ATGGAGACTG TGCTCCAGTA CTGCAAAGGA AAAAATCTCA AGAAACCAGC CCCCAAGTCT 
TACCTTATCC ACGCTGGTCT GGAGCCCCTG ACATTCACCA ATATGTTTCC CAGCTGGGAG 
CACAGAGAGG ACATCGCTGA GATCACAGAG ATGGACACGG AAGTTTCCAA TCAGATCACC 
CTCGTGGAAG ACGTCTTAGC CAAGCTCTGT AAAACCATTT ACCCGCTGGC CGACCTCCTG 
GCCAGGCCAC TCCCGGAGGG GGTCGATCCT CTGAAGCTTG AGATCTATCT CACCGACGAA 
GACTTCGAGT TTGCACTAGA CATGACGAGG GATGAATACA ACGCCCTGCC CGCCTGGAAG 
CAGGTGAACC TGAAGAAAGC AAAAGGCCTG TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( NM_021738.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_068506.2 Homo sapiens supervillin (SVIL), transcript variant 2, mRNA.

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CloneID	OHu13277
Clone ID Related Accession (Same CDS sequence)	NM_021738.3 , NM_021738.2
Accession Version	NM_021738.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	supervillin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF109135.1, AF051851.1, AL160060.11, BX457194.2, AF051850.1, BC092440.1, AI028650.1 and AL158167.15. On Jun 2, 2019 this sequence version replaced NM_021738.2. Transcript Variant: This variant (2) is alternatively spliced in the 5' portion of the gene, resulting in the longer, muscle-specific isoform (2) which has a different amino-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript that matched the reference genome was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF109135.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068506.2
CDS	753..7397
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	NM_021738.3
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021738.3

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( NM_021738.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_068506.2 Homo sapiens supervillin (SVIL), transcript variant 2, mRNA.

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CloneID	OHu13277
Clone ID Related Accession (Same CDS sequence)	NM_021738.3 , NM_021738.2
Accession Version	NM_021738.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	supervillin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF109135.1, AF051851.1, AL160060.11, BX457194.2, AF051850.1, BC092440.1 and AI028650.1. On Jul 3, 2007 this sequence version replaced NM_021738.1. Transcript Variant: This variant (2) is alternatively spliced in the 5' portion of the gene, resulting in the longer, muscle-specific isoform (2) which has a different amino-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript that matched the reference genome was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF109135.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068506.2
CDS	754..7398
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	NM_021738.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021738.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGCGGG TCACAGAAAG TCGAGAGAGC 
CAAATGACGA TTGAGGAGAG GAAGCAGCTC ATCACTGTGA GAGAGGAGGC CTGGAAGACG 
AGAGGCAGAG GAGCGGCCAA CGACTCGACC CAGTTCACTG TGGCTGGCAG GATGGTGAAG 
AAAGGTTTGG CGTCACCTAC TGCCATAACC CCAGTAGCCT CACCCATTTG CGGTAAAACA 
AGAGGCACCA CACCCGTTTC CAAACCCCTG GAAGATATCG AAGCCAGACC AGATATGCAG 
TTAGAATCGG ACCTGAAGTT GGACAGGCTG GAAACCTTTC TAAGAAGGCT GAATAACAAA 
GTTGGCGGGA TGCACGAAAC GGTGCTCACT GTCACCGGCA AATCTGTGAA GGAGGTGATG 
AAGCCAGATG ATGATGAAAC CTTTGCCAAA TTTTACCGCA GCGTGGATTA TAATATGCCA 
AGAAGTCCTG TGGAGATGGA TGAGGACTTC GATGTCATTT TCGATCCTTA TGCACCCAAA 
TTGACGTCTT CCGTGGCCGA GCACAAGCGG GCAGTTAGGC CCAAGCGCCG GGTTCAGGCC 
TCCAAAAACC CCCTGAAAAT GCTGGCGGCA AGAGAAGATC TCCTTCAGGA ATACACTGAG 
CAGAGATTAA ACGTTGCCTT CATGGAGTCA AAGCGGATGA AAGTAGAAAA GATGTCTTCC 
AACTCCAACT TCTCAGAAGT CACCCTGGCG GGTTTAGCCA GTAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAACTCTA ACAACAGCGC CGTGCCCTAC 
AAGAGGCTGA TGCTGTTGCA GATTAAAGGA AGAAGACATG TGCAGACCAG GCTGGTGGAA 
CCTCGAGCTT CGGCGCTCAA CAGTGGGGAC TGCTTCCTCC TGCTCTCTCC CCACTGCTGC 
TTCCTGTGGG TAGGAGAGTT TGCAAACGTC ATAGAAAAGG CGAAGGCCTC AGAACTTGCA 
ACTTTAATTC AGACAAAGAG GGAACTTGGT TGTAGAGCTA CTTATATCCA AACCATTGAA 
GAAGGAATTA ATACACACAC TCATGCAGCC AAAGACTTCT GGAAGCTTCT GGGTGGCCAA 
ACCAGTTACC AATCTGCTGG AGACCCAAAA GAAGATGAAC TCTATGAAGC AGCCATAATA 
GAAACTAACT GCATTTACCG TCTCATGGAT GACAAACTTG TTCCTGATGA CGACTACTGG 
GGGAAAATTC CGAAGTGCTC CCTTCTGCAA CCCAAAGAGG TACTGGTGTT TGATTTTGGT 
AGTGAAGTTT ACGTATGGCA TGGGAAAGAA GTCACATTAG CACAACGAAA AATAGCATTT 
CAGCTGGCAA AGCACTTATG GAATGGAACC TTTGACTATG AGAACTGTGA CATCAATCCC 
CTGGATCCTG GAGAATGCAA TCCGCTTATC CCCAGAAAAG GACAGGGGCG GCCCGACTGG 
GCGATATTTG GGAGACTTAC TGAACACAAT GAGACGATTT TGTTCAAAGA GAAGTTTCTG 
GATTGGACGG AACTGAAGAG ATCGAATGAG AAGAACCCCG GGGAACTTGC CCAGCACAAG 
GAAGACCCCA GGACTGATGT CAAGGCATAC GATGTGACAC GGATGGTGTC CATGCCCCAG 
ACGACAGCAG GCACCATCCT GGACGGAGTG AACGTCGGCC GTGGCTATGG CCTGGTGGAA 
GGACACGACA GGAGGCAGTT TGAGATCACC AGCGTTTCCG TGGATGTCTG GCACATCCTG 
GAATTCGACT ATAGCAGGCT CCCCAAACAA AGCATCGGGC AGTTCCATGA GGGGGATGCC 
TATGTGGTCA AGTGGAAGTT CATGGTGAGC ACGGCAGTGG GAAGTCGCCA GAAGGGAGAG 
CACTCGGTGA GGGCAGCCGG CAAAGAGAAG TGCGTCTACT TCTTCTGGCA AGGCCGGCAC 
TCCACCGTGA GTGAGAAGGG CACGTCGGCG CTGATGACGG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTTCT CCAGGGAAAG GAGCCCCCCT GTTTCCTGCA GTGTTTCCAG 
GGGGGGATGG TGGTGCACTC GGGGAGGCGG GAAGAGGAAG AAGAAAATGT GCAAAGTGAG 
TGGCGGCTGT ACTGCGTGCG TGGAGAGGTG CCCGTGGAAG GGAATTTGCT GGAAGTGGCC 
TGTCACTGTA GCAGCCTGAG GTCCAGAACT TCCATGGTGG TGCTTAACGT CAACAAGGCC 
CTCATCTACC TGTGGCACGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGAAGGACC 
GCTGCGAACA AGATCAAGGA ACAATGTCCC CTGGAAGCAG GACTGCATAG TAGCAGCAAA 
GTCACAATAC ACGAGTGTGA TGAAGGCTCC GAGCCACTCG GATTCTGGGA TGCCTTAGGA 
AGGAGAGACA GGAAAGCCTA CGATTGCATG CTTCAAGATC CTGGAAGTTT TAACTTCGCG 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGGGATTTTG CAGCCACAGA GTTTGTGTAC 
CCTGCCCGAG CCCCCTCTGT GGTCAGTTCC ATGCCCTTCC TGCAGGAAGA TCTGTACAGC 
GCGCCCCAGC CAGCACTTTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACTGGT TCCGCCCGCA TCCGCTGGGC CTCCGACCGG 
AAGAGTGCGA TGGAGACTGT GCTCCAGTAC TGCAAAGGAA AAAATCTCAA GAAACCAGCC 
CCCAAGTCTT ACCTTATCCA CGCTGGTCTG GAGCCCCTGA CATTCACCAA TATGTTTCCC 
AGCTGGGAGC ACAGAGAGGA CATCGCTGAG ATCACAGAGA TGGACACGGA AGTTTCCAAT 
CAGATCACCC TCGTGGAAGA CGTCTTAGCC AAGCTCTGTA AAACCATTTA CCCGCTGGCC 
GACCTCCTGG CCAGGCCACT CCCGGAGGGG GTCGATCCTC TGAAGCTTGA GATCTATCTC 
ACCGACGAAG ACTTCGAGTT TGCACTAGAC ATGACGAGGG ATGAATACAA CGCCCTGCCC 
GCCTGGAAGC AGGTGAACCT GAAGAAAGCA AAAGGCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519635.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517937.1 Homo sapiens supervillin (SVIL), transcript variant X12, mRNA.

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CloneID	OHu43844
Clone ID Related Accession (Same CDS sequence)	XM_011519635.1 , XM_005252570.2 , XM_005252573.3 , XM_011519634.1 , XM_011519635.2 , XM_005252573.2 , XM_005252571.3 , XM_005252571.4
Accession Version	XM_011519635.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517937.1
CDS	676..7446
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519635.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519635.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005252570.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252627.1 Homo sapiens supervillin (SVIL), transcript variant X8, mRNA.

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CloneID	OHu43844
Clone ID Related Accession (Same CDS sequence)	XM_011519635.1 , XM_005252570.2 , XM_005252573.3 , XM_011519634.1 , XM_011519635.2 , XM_005252573.2 , XM_005252571.3 , XM_005252571.4
Accession Version	XM_005252570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005252570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252627.1
CDS	651..7421
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005252570.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252570.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005252573.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252630.1 Homo sapiens supervillin (SVIL), transcript variant X10, mRNA.

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CloneID	OHu43844
Clone ID Related Accession (Same CDS sequence)	XM_011519635.1 , XM_005252570.2 , XM_005252573.3 , XM_011519634.1 , XM_011519635.2 , XM_005252573.2 , XM_005252571.3 , XM_005252571.4
Accession Version	XM_005252573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005252573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252630.1
CDS	1441..8211
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005252573.3
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252573.3

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519634.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517936.1 Homo sapiens supervillin (SVIL), transcript variant X9, mRNA.

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CloneID	OHu43844
Clone ID Related Accession (Same CDS sequence)	XM_011519635.1 , XM_005252570.2 , XM_005252573.3 , XM_011519634.1 , XM_011519635.2 , XM_005252573.2 , XM_005252571.3 , XM_005252571.4
Accession Version	XM_011519634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517936.1
CDS	709..7479
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519634.1
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519634.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_011519635.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517937.1 Homo sapiens supervillin (SVIL), transcript variant X11, mRNA.

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CloneID	OHu43844
Clone ID Related Accession (Same CDS sequence)	XM_011519635.1 , XM_005252570.2 , XM_005252573.3 , XM_011519634.1 , XM_011519635.2 , XM_005252573.2 , XM_005252571.3 , XM_005252571.4
Accession Version	XM_011519635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011519635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517937.1
CDS	1334..8104
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_011519635.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519635.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005252573.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252630.1 Homo sapiens supervillin (SVIL), transcript variant X11, mRNA.

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CloneID	OHu43844
Clone ID Related Accession (Same CDS sequence)	XM_011519635.1 , XM_005252570.2 , XM_005252573.3 , XM_011519634.1 , XM_011519635.2 , XM_005252573.2 , XM_005252571.3 , XM_005252571.4
Accession Version	XM_005252573.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005252573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252630.1
CDS	783..7553
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005252573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252573.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005252571.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252628.1 Homo sapiens supervillin (SVIL), transcript variant X8, mRNA.

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CloneID	OHu43844
Clone ID Related Accession (Same CDS sequence)	XM_011519635.1 , XM_005252570.2 , XM_005252573.3 , XM_011519634.1 , XM_011519635.2 , XM_005252573.2 , XM_005252571.3 , XM_005252571.4
Accession Version	XM_005252571.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	supervillin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252571.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252628.1
CDS	577..7347
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005252571.3
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252571.3

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_005252571.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005252628.1 Homo sapiens supervillin (SVIL), transcript variant X7, mRNA.

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CloneID	OHu43844
Clone ID Related Accession (Same CDS sequence)	XM_011519635.1 , XM_005252570.2 , XM_005252573.3 , XM_011519634.1 , XM_011519635.2 , XM_005252573.2 , XM_005252571.3 , XM_005252571.4
Accession Version	XM_005252571.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005252571.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005252628.1
CDS	448..7218
Translation	MKRKERIARRLEGIENDTQPILLQSCTGLVTHRLLEEDTPRYMRASDPASPHIGRSNEEEETSDSSLEKQTRSKYCTETSGVHGDSPYGSGTMDTHSLESKAERIARYKAERRRQLAEKYGLTLDPEADSEYLSRYTKSRKEPDAVEKRGGKSDKQEESSRDASSLYPGTETMGLRTCAGESKDYALHVGDGSSDPEVLLNIENQRRGQELSATRQAHDLSPAAESSSTFSFSGRDSSFTEVPRSPKHAHSSSLQQAASRSPSFGDPQLSPEARPSTGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVKEESARNSPELASESVTQRRHQPAPVHYVSFQSEHSAFDRVPSKAAGSTRQPIRGYVQPADTGHTAKLVTPETPENASECSWVASATQNVPKPPSLTVLEGDGRDSPVLHVCESKAEEEEGEGEGEEKEEDVCFTEALEQSKKTLLALEGDGLVRSPEDPSRNEDFGKPAVSTVTLEHQKELENVAQPPQAPHQPTERTGRSEMVLYIQSEPVSQDAKPTGHNREASKKRKVRTRSLSDFTGPPQLQALKYKDPASRRELELPSSKTEGPYGEISMLDTKVSVAQLRSAFLASANACRRPELKSRVERSAEGPGLPTGVERERGSRKPRRYFSPGESRKTSERFRTQPITSAERKESDRCTSHSETPTVDDEEKVDERAKLSVAAKRLLFREMEKSFDEQNVPKRRSRNTAVEQRLRRLQDRSLTQPITTEEVVIAATEPIPASCSGGTHPVMARLPSPTVARSAVQPARLQASAHQKALAKDQTNEGKELAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRIDTRQRRMNARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRAREGDSHKESKYAVPRRGSLERANPPITHLGDEPKEFSMAKMNAQGNLDLRDRLPFEEKVEVENVMKRKFSLRAAEFGEPTSEQTGTAAGKTIAQTTAPVSWKPQDSSEQPQEKLCKNPCAMFAAGEIKTPTGEGLLDSPSKTMSIKERLALLKKSGEEDWRNRLSRRQEGGKAPASSLHTQEAGRSLIKKEEGGVADDSAISNLLWEPVYASTYSPAIPAAHKYLSFVSINQRVTESRESQMTIEERKQLITVREEAWKTRGRGAANDSTQFTVAGRMVKKGLASPTAITPVASPICGKTRGTTPVSKPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMHETVLTVTGKSVKEVMKPDDDETFAKFYRSVDYNMPRSPVEMDEDFDVIFDPYAPKLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFMESKRMKVEKMSSNSNFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKRLMLLQIKGRRHVQTRLVEPRASALNSGDCFLLLSPHCCFLWVGEFANVIEKAKASELATLIQTKRELGCRATYIQTIEEGINTHTHAAKDFWKLLGGQTSYQSAGDPKEDELYEAAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQPKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRSNEKNPGELAQHKEDPRTDVKAYDVTRMVSMPQTTAGTILDGVNVGRGYGLVEGHDRRQFEITSVSVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFMVSTAVGSRQKGEHSVRAAGKEKCVYFFWQGRHSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENVQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVNKALIYLWHGCKAQAHTKEVGRTAANKIKEQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGSFNFAPRLFILSSSSGDFAATEFVYPARAPSVVSSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCKGKNLKKPAPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_005252571.4
Organism	Homo sapiens(human)
Definition	Homo sapiens supervillin (SVIL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005252571.4

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCAGGCGC CTGGAAGGGA TTGAAAATGA CACTCAGCCC 
ATCCTCTTGC AGAGCTGCAC AGGATTGGTG ACTCACCGCC TGCTGGAGGA AGACACCCCT 
CGATACATGA GAGCCAGCGA CCCTGCCAGC CCCCACATCG GCCGATCAAA TGAAGAGGAG 
GAAACTTCTG ATTCTTCTCT AGAAAAGCAA ACTCGATCCA AATACTGCAC AGAAACCTCC 
GGTGTCCACG GTGACTCACC CTATGGTTCG GGTACCATGG ACACCCACAG TCTGGAGTCC 
AAAGCCGAAA GAATTGCAAG GTACAAAGCA GAAAGAAGGC GACAGCTGGC AGAGAAGTAT 
GGGCTGACTC TGGATCCCGA GGCCGACTCC GAGTATTTAT CCCGCTATAC CAAGTCCAGG 
AAGGAGCCTG ATGCTGTCGA GAAGCGGGGA GGAAAAAGTG ACAAACAGGA AGAGTCAAGC 
AGAGATGCTA GTTCTCTGTA CCCCGGGACC GAGACGATGG GGCTCAGGAC CTGTGCCGGT 
GAATCCAAGG ACTATGCCCT CCATGTGGGT GACGGCTCTT CCGACCCGGA GGTGCTGCTG 
AACATAGAAA ACCAAAGACG AGGTCAAGAG CTGAGTGCCA CCCGGCAGGC CCATGACCTG 
TCCCCAGCAG CCGAGAGTTC CTCGACCTTC TCTTTCTCTG GGCGAGACTC CTCCTTCACT 
GAAGTGCCAC GGTCCCCCAA GCACGCCCAC AGCTCCTCCC TGCAGCAGGC AGCCTCCCGG 
AGCCCCTCCT TTGGTGACCC ACAGCTATCC CCTGAGGCCC GACCCAGTAC AGGGAAACCC 
AAACATGAGT GGTTTCTCCA GAAAGATTCC GAAGGGGACA CACCTTCACT TATCAACTGG 
CCTTCCAGAG TTAAAGTTAG AGAAAAATTG GTGAAAGAGG AAAGTGCTCG AAACAGCCCT 
GAACTCGCCT CAGAGTCCGT AACTCAGAGG AGACACCAGC CAGCGCCAGT CCATTACGTG 
TCATTTCAGT CTGAGCACTC AGCCTTTGAT AGGGTCCCCA GCAAGGCAGC AGGCTCTACA 
CGACAGCCAA TCCGTGGCTA TGTCCAACCC GCAGATACCG GTCACACCGC CAAGCTAGTG 
ACGCCAGAAA CCCCAGAAAA TGCATCTGAG TGTAGCTGGG TAGCATCAGC CACCCAGAAT 
GTCCCCAAAC CTCCCAGCTT GACGGTTCTA GAAGGTGACG GAAGGGATAG CCCAGTTCTC 
CATGTCTGCG AGTCAAAAGC AGAAGAAGAA GAAGGGGAAG GAGAAGGAGA AGAAAAAGAA 
GAAGATGTGT GCTTCACTGA AGCTCTCGAG CAAAGCAAGA AAACCCTACT GGCTTTGGAG 
GGTGATGGGC TAGTGAGAAG CCCAGAAGAT CCCTCTAGAA ATGAGGACTT TGGTAAGCCT 
GCTGTGAGCA CAGTCACCTT AGAGCATCAG AAGGAACTGG AAAACGTGGC ACAACCCCCT 
CAAGCTCCGC ACCAGCCCAC TGAGAGGACA GGCAGGAGCG AGATGGTTCT CTACATTCAA 
AGTGAGCCTG TGTCCCAAGA CGCCAAACCA ACTGGTCACA ACAGGGAAGC CTCGAAAAAG 
CGCAAGGTCC GTACCCGCTC TCTGTCAGAT TTCACAGGCC CCCCTCAGCT CCAGGCCTTG 
AAGTATAAGG ACCCAGCTTC CAGGAGAGAG CTGGAGCTGC CCAGCTCCAA GACCGAAGGG 
CCTTATGGGG AGATCAGCAT GCTGGACACA AAAGTCTCTG TCGCCCAGCT CCGAAGTGCG 
TTCCTGGCAT CTGCCAACGC CTGCAGGAGA CCTGAACTCA AATCACGGGT GGAGAGGTCG 
GCTGAAGGAC CTGGCTTGCC CACCGGTGTG GAACGGGAGA GAGGGTCCCG GAAACCAAGA 
CGCTATTTTT CTCCTGGTGA AAGTAGAAAA ACTTCCGAGA GATTTAGAAC CCAACCTATA 
ACTTCAGCAG AACGAAAGGA ATCGGATAGG TGCACTTCAC ATTCAGAAAC GCCAACTGTC 
GATGATGAAG AAAAGGTGGA TGAACGAGCC AAGCTGAGCG TCGCCGCCAA GAGGTTGCTT 
TTCAGGGAGA TGGAAAAATC TTTTGATGAA CAAAATGTTC CAAAGCGACG CTCAAGAAAC 
ACAGCTGTGG AGCAGAGGCT ACGCCGTCTG CAGGACAGGT CCCTCACCCA GCCCATCACC 
ACTGAAGAGG TGGTCATCGC AGCCACTGAA CCTATCCCCG CTTCGTGTTC TGGGGGCACC 
CACCCTGTAA TGGCGAGACT TCCTAGCCCC ACTGTAGCTA GGAGCGCTGT GCAGCCTGCC 
AGATTGCAGG CCTCTGCTCA CCAAAAGGCC TTAGCCAAGG ACCAGACAAA TGAGGGCAAA 
GAGCTTGCTG AGCAAGGAGA ACCTGATTCC TCCACTCTAA GCTTGGCCGA AAAGTTGGCC 
TTGTTTAACA AATTGTCCCA GCCAGTCTCA AAAGCGATTT CTACCCGGAA CAGAATAGAC 
ACGAGACAGA GGAGAATGAA CGCTCGCTAT CAAACTCAGC CAGTCACACT GGGAGAGGTG 
GAGCAGGTGC AGAGTGGAAA GCTCATTCCT TTCTCACCTG CCGTGAACAC ATCAGTGTCT 
ACCGTAGCAT CCACGGTTGC TCCAATGTAT GCCGGAGATC TTCGCACAAA GCCACCTCTT 
GACCACAATG CAAGTGCCAC TGACTATAAG TTTTCTTCTT CAATAGAAAA TTCGGACTCT 
CCAGTTAGAA GCATTCTGAA ATCGCAAGCT TGGCAGCCTT TGGTAGAGGG TAGCGAGAAC 
AAGGGAATGT TGAGAGAATA TGGAGAGACA GAAAGCAAGA GAGCTTTGAC AGGTCGAGAC 
AGTGGGATGG AGAAGTATGG GTCCTTTGAG GAAGCAGAAG CATCCTACCC CATCCTGAAC 
CGAGCCAGGG AAGGAGACAG CCATAAGGAA TCTAAATATG CTGTTCCCAG AAGAGGAAGC 
CTGGAACGGG CGAACCCTCC CATCACCCAC CTCGGGGATG AACCGAAGGA ATTTTCCATG 
GCTAAAATGA ATGCACAAGG AAACTTGGAC TTGAGGGACA GGCTGCCCTT TGAAGAGAAG 
GTGGAGGTGG AGAATGTTAT GAAAAGGAAG TTTTCACTAA GAGCGGCAGA GTTCGGGGAG 
CCCACTTCCG AGCAGACGGG GACAGCTGCT GGGAAAACTA TTGCTCAAAC CACAGCCCCC 
GTGTCCTGGA AGCCCCAGGA TTCTTCGGAA CAGCCACAGG AGAAGCTCTG CAAGAATCCA 
TGTGCGATGT TTGCTGCTGG AGAGATCAAA ACGCCGACAG GGGAGGGCCT TCTTGACTCA 
CCCAGCAAAA CCATGTCTAT TAAAGAAAGA TTGGCACTGT TGAAGAAAAG CGGGGAGGAA 
GATTGGAGAA ACAGACTCAG CAGGAGGCAG GAGGGCGGCA AGGCGCCGGC CAGCAGCCTG 
CACACCCAGG AAGCAGGGCG GTCCCTCATC AAGAAGGAAG AAGGAGGAGT TGCGGATGAT 
AGTGCCATTT CTAATCTGCT TTGGGAACCT GTATATGCTT CTACTTATTC TCCTGCTATT 
CCTGCTGCCC ATAAATACCT GTCTTTTGTG TCGATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAAA TGACGATTGA GGAGAGGAAG CAGCTCATCA CTGTGAGAGA GGAGGCCTGG 
AAGACGAGAG GCAGAGGAGC GGCCAACGAC TCGACCCAGT TCACTGTGGC TGGCAGGATG 
GTGAAGAAAG GTTTGGCGTC ACCTACTGCC ATAACCCCAG TAGCCTCACC CATTTGCGGT 
AAAACAAGAG GCACCACACC CGTTTCCAAA CCCCTGGAAG ATATCGAAGC CAGACCAGAT 
ATGCAGTTAG AATCGGACCT GAAGTTGGAC AGGCTGGAAA CCTTTCTAAG AAGGCTGAAT 
AACAAAGTTG GCGGGATGCA CGAAACGGTG CTCACTGTCA CCGGCAAATC TGTGAAGGAG 
GTGATGAAGC CAGATGATGA TGAAACCTTT GCCAAATTTT ACCGCAGCGT GGATTATAAT 
ATGCCAAGAA GTCCTGTGGA GATGGATGAG GACTTCGATG TCATTTTCGA TCCTTATGCA 
CCCAAATTGA CGTCTTCCGT GGCCGAGCAC AAGCGGGCAG TTAGGCCCAA GCGCCGGGTT 
CAGGCCTCCA AAAACCCCCT GAAAATGCTG GCGGCAAGAG AAGATCTCCT TCAGGAATAC 
ACTGAGCAGA GATTAAACGT TGCCTTCATG GAGTCAAAGC GGATGAAAGT AGAAAAGATG 
TCTTCCAACT CCAACTTCTC AGAAGTCACC CTGGCGGGTT TAGCCAGTAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ACTCTAACAA CAGCGCCGTG 
CCCTACAAGA GGCTGATGCT GTTGCAGATT AAAGGAAGAA GACATGTGCA GACCAGGCTG 
GTGGAACCTC GAGCTTCGGC GCTCAACAGT GGGGACTGCT TCCTCCTGCT CTCTCCCCAC 
TGCTGCTTCC TGTGGGTAGG AGAGTTTGCA AACGTCATAG AAAAGGCGAA GGCCTCAGAA 
CTTGCAACTT TAATTCAGAC AAAGAGGGAA CTTGGTTGTA GAGCTACTTA TATCCAAACC 
ATTGAAGAAG GAATTAATAC ACACACTCAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGT 
GGCCAAACCA GTTACCAATC TGCTGGAGAC CCAAAAGAAG ATGAACTCTA TGAAGCAGCC 
ATAATAGAAA CTAACTGCAT TTACCGTCTC ATGGATGACA AACTTGTTCC TGATGACGAC 
TACTGGGGGA AAATTCCGAA GTGCTCCCTT CTGCAACCCA AAGAGGTACT GGTGTTTGAT 
TTTGGTAGTG AAGTTTACGT ATGGCATGGG AAAGAAGTCA CATTAGCACA ACGAAAAATA 
GCATTTCAGC TGGCAAAGCA CTTATGGAAT GGAACCTTTG ACTATGAGAA CTGTGACATC 
AATCCCCTGG ATCCTGGAGA ATGCAATCCG CTTATCCCCA GAAAAGGACA GGGGCGGCCC 
GACTGGGCGA TATTTGGGAG ACTTACTGAA CACAATGAGA CGATTTTGTT CAAAGAGAAG 
TTTCTGGATT GGACGGAACT GAAGAGATCG AATGAGAAGA ACCCCGGGGA ACTTGCCCAG 
CACAAGGAAG ACCCCAGGAC TGATGTCAAG GCATACGATG TGACACGGAT GGTGTCCATG 
CCCCAGACGA CAGCAGGCAC CATCCTGGAC GGAGTGAACG TCGGCCGTGG CTATGGCCTG 
GTGGAAGGAC ACGACAGGAG GCAGTTTGAG ATCACCAGCG TTTCCGTGGA TGTCTGGCAC 
ATCCTGGAAT TCGACTATAG CAGGCTCCCC AAACAAAGCA TCGGGCAGTT CCATGAGGGG 
GATGCCTATG TGGTCAAGTG GAAGTTCATG GTGAGCACGG CAGTGGGAAG TCGCCAGAAG 
GGAGAGCACT CGGTGAGGGC AGCCGGCAAA GAGAAGTGCG TCTACTTCTT CTGGCAAGGC 
CGGCACTCCA CCGTGAGTGA GAAGGGCACG TCGGCGCTGA TGACGGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTTCTCCAG GGAAAGGAGC CCCCCTGTTT CCTGCAGTGT 
TTCCAGGGGG GGATGGTGGT GCACTCGGGG AGGCGGGAAG AGGAAGAAGA AAATGTGCAA 
AGTGAGTGGC GGCTGTACTG CGTGCGTGGA GAGGTGCCCG TGGAAGGGAA TTTGCTGGAA 
GTGGCCTGTC ACTGTAGCAG CCTGAGGTCC AGAACTTCCA TGGTGGTGCT TAACGTCAAC 
AAGGCCCTCA TCTACCTGTG GCACGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
AGGACCGCTG CGAACAAGAT CAAGGAACAA TGTCCCCTGG AAGCAGGACT GCATAGTAGC 
AGCAAAGTCA CAATACACGA GTGTGATGAA GGCTCCGAGC CACTCGGATT CTGGGATGCC 
TTAGGAAGGA GAGACAGGAA AGCCTACGAT TGCATGCTTC AAGATCCTGG AAGTTTTAAC 
TTCGCGCCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGGG ATTTTGCAGC CACAGAGTTT 
GTGTACCCTG CCCGAGCCCC CTCTGTGGTC AGTTCCATGC CCTTCCTGCA GGAAGATCTG 
TACAGCGCGC CCCAGCCAGC ACTTTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACTGGTTCCG CCCGCATCCG CTGGGCCTCC 
GACCGGAAGA GTGCGATGGA GACTGTGCTC CAGTACTGCA AAGGAAAAAA TCTCAAGAAA 
CCAGCCCCCA AGTCTTACCT TATCCACGCT GGTCTGGAGC CCCTGACATT CACCAATATG 
TTTCCCAGCT GGGAGCACAG AGAGGACATC GCTGAGATCA CAGAGATGGA CACGGAAGTT 
TCCAATCAGA TCACCCTCGT GGAAGACGTC TTAGCCAAGC TCTGTAAAAC CATTTACCCG 
CTGGCCGACC TCCTGGCCAG GCCACTCCCG GAGGGGGTCG ATCCTCTGAA GCTTGAGATC 
TATCTCACCG ACGAAGACTT CGAGTTTGCA CTAGACATGA CGAGGGATGA ATACAACGCC 
CTGCCCGCCT GGAAGCAGGT GAACCTGAAG AAAGCAAAAG GCCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle.
Human molecular genetics24(9)2470-81(2015 May)
Spinazzola JM,Smith TC,Liu M,Luna EJ,Barton ER

A specific LSD1/KDM1A isoform regulates neuronal differentiation through H3K9 demethylation.
Molecular cell57(6)957-70(2015 Mar)
Laurent B,Ruitu L,Murn J,Hempel K,Ferrao R,Xiang Y,Liu S,Garcia BA,Wu H,Wu F,Steen H,Shi Y

Supervillin binding to myosin II and synergism with anillin are required for cytokinesis.
Molecular biology of the cell24(23)3603-19(2013 Dec)
Smith TC,Fridy PC,Li Y,Basil S,Arjun S,Friesen RM,Leszyk J,Chait BT,Rout MP,Luna EJ

The role of PLK1-phosphorylated SVIL in myosin II activation and cytokinetic furrowing.
Journal of cell science126(Pt 16)3627-37(2013 Aug)
Hasegawa H,Hyodo T,Asano E,Ito S,Maeda M,Kuribayashi H,Natsume A,Wakabayashi T,Hamaguchi M,Senga T

Supervillin-mediated suppression of p53 protein enhances cell survival.
The Journal of biological chemistry288(11)7918-29(2013 Mar)
Fang Z,Luna EJ

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SVIL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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