SVIL gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_025000268.1	XP_024856036.1	supervillin isoform X6
XM_025000270.1	XP_024856038.1	supervillin isoform X8
XM_025000272.1	XP_024856040.1	supervillin isoform X9
XM_025000278.1	XP_024856046.1	supervillin isoform X9
XM_025000279.1	XP_024856047.1	supervillin isoform X10
XM_025000280.1	XP_024856048.1	supervillin isoform X11
XM_025000282.1	XP_024856050.1	supervillin isoform X12
XM_025000283.1	XP_024856051.1	supervillin isoform X9
XM_025000286.1	XP_024856054.1	supervillin isoform X15
XM_025000287.1	XP_024856055.1	supervillin isoform X16
XM_025000289.1	XP_024856057.1	supervillin isoform X18
XM_025000267.1	XP_024856035.1	supervillin isoform X5
XM_025000273.1	XP_024856041.1	supervillin isoform X9
XM_025000277.1	XP_024856045.1	supervillin isoform X9
XM_025000285.1	XP_024856053.1	supervillin isoform X14
XM_025000263.1	XP_024856031.1	supervillin isoform X1
XM_025000264.1	XP_024856032.1	supervillin isoform X2
XM_025000265.1	XP_024856033.1	supervillin isoform X3
XM_025000266.1	XP_024856034.1	supervillin isoform X4
XM_025000269.1	XP_024856037.1	supervillin isoform X7
XM_025000271.1	XP_024856039.1	supervillin isoform X9
XM_025000275.1	XP_024856043.1	supervillin isoform X9
XM_025000281.1	XP_024856049.1	supervillin isoform X9
XM_025000290.1	XP_024856058.1	supervillin isoform X19
NM_174190.2	NP_776615.1	supervillin
XM_025000284.1	XP_024856052.1	supervillin isoform X13
XM_025000292.1	XP_024856060.1	supervillin isoform X21
XM_025000293.1	XP_024856061.1	supervillin isoform X21
XM_025000274.1	XP_024856042.1	supervillin isoform X9
XM_025000276.1	XP_024856044.1	supervillin isoform X9
XM_025000288.1	XP_024856056.1	supervillin isoform X17
XM_025000291.1	XP_024856059.1	supervillin isoform X20

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa221418	XM_025000286.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221419	XM_025000287.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221420	XM_025000288.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221421	XM_025000289.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221422	XM_025000290.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221423	XM_025000291.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221424	XM_025000292.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa221424	XM_025000293.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa43786	NM_174190.2 Latest version!	Bos taurus supervillin (SVIL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa221404	XM_025000263.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221405	XM_025000264.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221406	XM_025000265.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221407	XM_025000266.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221408	XM_025000267.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221409	XM_025000268.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221410	XM_025000269.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221411	XM_025000270.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000273.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000276.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000281.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000275.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000278.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000283.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000272.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000277.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000274.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221412	XM_025000271.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221413	XM_025000279.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221414	XM_025000280.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221415	XM_025000282.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221416	XM_025000284.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa221417	XM_025000285.1 Latest version!	Bos taurus supervillin (SVIL), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

SVIL ( XM_025000286.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856054.1 Bos taurus supervillin (SVIL), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OBa221418
Clone ID Related Accession (Same CDS sequence)	XM_025000286.1
Accession Version	XM_025000286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGGTCAAC TTCAAATTCA 
GAAATGCCAG CTGCCGAGGA TGAAGAAAAG GTCGATGAGC GAGCGAGGCT GAGCGTCGCT 
GCTAAGAGGC TGCTCTTCAG GGAGATGGAG AAGTCGTTTG ACGAGAAGAG CGTCCCAAAG 
CGCCGCTCCC GCAATGCGGC CGTGGAGCAG AGGCTGCGGC GCCTCCAGGA CCGGTCCCAC 
ACGCAGCCCG TCACCACCGA GGAGGTGGTC ATTGCAGCCA CTGAACCTAC CCCCGCTTCG 
TGCTCAGTGG CCACCCACCC TGTAATGACA AGACATCCTA GCCCCACTGT AGCTAAGAGT 
CCCGTGCAGC CTGCCAGATT GCAGGCGTCT GCTCACCAAA AGGCACTAGC CAGAGACCAG 
ACAAACGAGA GCAAAGATTC TGCTGAGCAG GGAGAACCTG ACTCCTCCAC TCTGAGCTTA 
GCGGAGAAGC TGGCCTTGTT TAACAAACTA TCTCAGCCGG TCTCAAAAGC CATTTCAACA 
CGGAACAGAT TAGACATGAG ACAGAGGAGG ATGAATGCTC GTTATCAAAC CCAGCCGGTC 
ACGCTTGGAG AAGTGGAACA GGTGCAGAGT GGCAAGCTCA TGGCTTTCTC TCCCACCATT 
AACACTTCCG TGTCCACCGT GGCATCTACC GTCCCCCCCA TGTATGCAGG AAATCTTCGG 
ACAAAGCCAC TTCCAGATGA CAGCTTTGGT GCCACTGAGC AGAAGTTTGC TTCTTCGCTA 
GAAAACTCCG ACTCCCCAGT TAGAAGCATC CTGAAATCCC AAGGCTGGCA GCCCTCGGTC 
GAGGGTGCTG GGAGCAAAGC AATGTTGAGA GAATTTGAAG AGACAGAACG CAAGGGAGGC 
TTGACAGGTG GAGATGGCGG GGTTACAAAG TATGGGTCCT TCGAGGAAGC AGAGCTGTCC 
TACCCCGTCC TCAGCAGAGT CCGGGAGGGA GACAACCATA AAGAGGCCAT CTATGCCCTT 
CCGAGGAAAG GAAGCCTGGA GCTCGCCCAT CCTCCCATCG CCCAGCTTGG TGATGACCTG 
AAGGAATTTT CCACCCCAAA GAGCACCATG CAGGCAAGCC CAGACTGGAA GGAGAGGCAG 
CTCTTTGAAG AGAAGGTGGA CTTGGAGAAT GTCACAAAGA GGAAGTTTTC GCTGAAAGCC 
GCGGAGTTCG GGGAACCCAC TTCTGAGCAG ACCGGCGCGG CTGCTGGGAA ACCGGCTGCT 
CCGACTGCAA CCCCGGTGTC CTGGAAGCCG CAGGATCCCT CCGAACAGCC TCAGGAGAAG 
CGGTATCAGA GCCCGTGTGC GATGTTTGCT GCTGGAGAGA TCAAAGCCCC GGCGGTGGAG 
GGCAGCCTCG ACTCCCCCAG CAAAACCATG TCCATTAAAG AAAGATTAGC ACTGTTGAAG 
AAGAGCGGTG AGGAAGACTG GAGAAACAGA CTCAACAGAA AGCAGGAATA CGGCAAAGCC 
TCCATCACCA GCAGCCTGCA CATCCAAGAG ACGGAGCAGT CACTCAAGAA GAAGGAAGAA 
GGAGGATTTA CAGATGATAA TGACATTTCT AATCTGCTTT GGGAACCTGT ATACGCTTCT 
ACTTATTCTC CTGCTCTACC TGCTGCCCAT AAATACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATTGAAG AGAGAAAGCA TCTCATCACC 
GTGAGAGAGG ACGCCTGGAA GACCAGGGGC AAAGGAGCAG CCAACGACTC TACCCAGTTT 
ACTGTGGCCG GCAGGATGGT GAAGCGAGGT CTGGCGTCCC CCACTGCCAT CACCCCGGTA 
GCATCCCCTG TTTCCAGCAA AGCAAGGGGC ACTACACCAG TTTCCAGACC CCTGGAAGAT 
ATCGAAGCCA GACCAGACAT GCAGTTAGAG TCGGACCTCA AGCTGGACAG GCTGGAAACC 
TTCCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAAG AAACAGTCCT CACTGTCACT 
GGGAAATCGG TGAAAGAGGT GATGAAGCCG GACGATGATG AAACCTTTGC CAAGTTTTAC 
CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC CCCGTAGAGC TGGACGAGGA TTTCGATGTC 
ATTTTTGATC CTTATGCCCC CAGGTTGACA TCCTCGGTGG CTGAGCACAA GCGTGCCGTG 
CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA AACCCCCTGA AGATGCTGGC CGCGAGGGAA 
GATCTCCTGC AGGAGTACAC TGAGCAGAGA CTGAACGTGG CCTTCGTGGA GTCAAAGCGG 
ATGAAAGTCG AAAAGTTGTC CGCCAACTCC AGCTTCTCAG AAGTCACCCT GGCGGGGTTA 
GCCAGCAAAG AAAACTTCAG CAACGTCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAACA GTGCAGTGCC CTACAAGAAG CTGATGCTGC TGCAGGTTAA AGGAAGAAGA 
CACGTGCAGA CGCGGCTGGT GGAGCCTCGC GCCCCCTCCC TCAACAGCGG GGACTGCTTC 
CTCCTGCTCT CGCCCCATCA CTGCTTCCTG TGGGTCGGAG AGTTCGCCAA CGTGATCGAG 
AAGGCGAAGG CCTCAGAACT TGCGAGTTTA ATTCAGACCA AGAGGGAACT TGGTTGTAGA 
GCAACTTACA TCCAGACTGT TGAAGAAGGA ATTAATACAC ACACACATGC AGCCAAAGAC 
TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT TACCAATCCG CCGGAGACCC AAAGGAGGAT 
GAGCTCTATG AAACGGCCAT AATAGAGACC AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTCGTTCCTG ATGATGACTA CTGGGGGAAG ATCCCAAAGT GCTCCCTCCT GCAATCAAAA 
GAGGTACTGG TGTTTGATTT TGGGAGTGAA GTTTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCACAAC GGAAAATCGC CTTTCAGCTG GCAAAGCACT TGTGGAATGG AACCTTTGAC 
TACGAGAATT GTGACATTAA CCCGCTGGAT CCTGGGGAAT GCAATCCCCT CATTCCCAGG 
AAAGGACAGG GGCGGCCCGA CTGGGCAATA TTTGGGAGAC TTACAGAACA CAATGAGACG 
ATCTTGTTCA AAGAGAAATT CCTTGATTGG ACGGAACTGA AGAGACCTAA CGAGAAGAAC 
GCCAGCGAAC TCGCGCAGCA CAAGGACGAT GCCCGGGCAG AGGTCAAGCC TTACGACGTG 
ACGCGGATGG TGCCCGTGCC CCAGACAACA GCCGGCACCG TGCTGGATGG GGTCAACGTG 
GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC GACCGGAGGC AGTTCGAGAT CGCCAGCATC 
TCAGTGGACG TCTGGCACAT CCTGGAGTTC GACTACAGCA GGCTCCCCAA GCAGAGCATC 
GGGCAGTTCC ACGAAGGCGA TGCCTACGTG GTGAAGTGGA AGTTCATCGT GAGCACTGCA 
GTGGGAAGCC GGCAGAAGGG GGAGCACTCG GTCAGGGTGG CTGGCAAAGA GAAATGTGTC 
TACTTCTTCT GGCAAGGCCG CCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGACGAGGA AAGGGGGGCC CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC 
CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG 
GAGGAAGAGA ACACACAGAG CGAGTGGCGG CTGTACTGTG TGCGCGGAGA GGTGCCTGTG 
GAGGGGAACT TGCTGGAGGT GGCCTGTCAC TGCAGCAGCC TCAGGTCTAG GACGTCCATG 
GTTGTCCTCA ACGTCCACAA AGCCCTCATC TACCTGTGGC ATGGATGCAA AGCCCAGGCC 
CACACGAAGG AGGTCGGACG GACTGCAGCC AATAAAATCA AGGACCAATG TCCCCTGGAG 
GCCGGGCTGC ACAGTAGCAG CAAAGTGACA ATTCATGAGT GTGATGAAGG CTCGGAGCCC 
CTGGGGTTCT GGGACGCTCT GGGGAGGAGA GACCGGAAAG CCTACGACTG CATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCCCGC CTGTTCATCC TCAGCAGCTC CTCTGGTGAC 
TTCTCAGCCA CAGAGTTCAT GTATCCCGCC CGAGACCCCT CTGTGGTCAA CTCTATGCCC 
TTCCTGCAGG AAGACCTATA CAGCGCCCCA CAGCCAGCAC TCTTCCTTGT TGACAATCAC 
CACGAGGTGT ACCTCTGGCA AGGCTGGTGG CCCATCGAGA ACAAGATCAC GGGCTCCGCG 
CGCATCCGCT GGGCCTCGGA CCGGAAGAGC GCCATGGAGA CAGTGCTGCA GTACTGCCGA 
GGGAAAAACC TGAAGAAGCC GCCCCCCAAG TCTTACCTCA TCCACGCTGG CCTGGAACCC 
CTGACCTTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AAGACATTGC AGAGATCACG 
GAGATGGACA CGGAAGTTTC AAATCAGATC ACCCTAGTGG AGGATGTCTT AGCCAAACTG 
TGTAAGACCA TTTACCCACT GGCCGACCTC CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC 
CCTCTGAAGC TGGAGATCTA TCTCACCGAC GAGGACTTCG AGTTTGCACT AGACATGACC 
CGAGACGAGT ACAATGCACT GCCCGCTTGG AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856054.1
CDS	356..6304
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000286.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000286.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGGTCAAC TTCAAATTCA 
GAAATGCCAG CTGCCGAGGA TGAAGAAAAG GTCGATGAGC GAGCGAGGCT GAGCGTCGCT 
GCTAAGAGGC TGCTCTTCAG GGAGATGGAG AAGTCGTTTG ACGAGAAGAG CGTCCCAAAG 
CGCCGCTCCC GCAATGCGGC CGTGGAGCAG AGGCTGCGGC GCCTCCAGGA CCGGTCCCAC 
ACGCAGCCCG TCACCACCGA GGAGGTGGTC ATTGCAGCCA CTGAACCTAC CCCCGCTTCG 
TGCTCAGTGG CCACCCACCC TGTAATGACA AGACATCCTA GCCCCACTGT AGCTAAGAGT 
CCCGTGCAGC CTGCCAGATT GCAGGCGTCT GCTCACCAAA AGGCACTAGC CAGAGACCAG 
ACAAACGAGA GCAAAGATTC TGCTGAGCAG GGAGAACCTG ACTCCTCCAC TCTGAGCTTA 
GCGGAGAAGC TGGCCTTGTT TAACAAACTA TCTCAGCCGG TCTCAAAAGC CATTTCAACA 
CGGAACAGAT TAGACATGAG ACAGAGGAGG ATGAATGCTC GTTATCAAAC CCAGCCGGTC 
ACGCTTGGAG AAGTGGAACA GGTGCAGAGT GGCAAGCTCA TGGCTTTCTC TCCCACCATT 
AACACTTCCG TGTCCACCGT GGCATCTACC GTCCCCCCCA TGTATGCAGG AAATCTTCGG 
ACAAAGCCAC TTCCAGATGA CAGCTTTGGT GCCACTGAGC AGAAGTTTGC TTCTTCGCTA 
GAAAACTCCG ACTCCCCAGT TAGAAGCATC CTGAAATCCC AAGGCTGGCA GCCCTCGGTC 
GAGGGTGCTG GGAGCAAAGC AATGTTGAGA GAATTTGAAG AGACAGAACG CAAGGGAGGC 
TTGACAGGTG GAGATGGCGG GGTTACAAAG TATGGGTCCT TCGAGGAAGC AGAGCTGTCC 
TACCCCGTCC TCAGCAGAGT CCGGGAGGGA GACAACCATA AAGAGGCCAT CTATGCCCTT 
CCGAGGAAAG GAAGCCTGGA GCTCGCCCAT CCTCCCATCG CCCAGCTTGG TGATGACCTG 
AAGGAATTTT CCACCCCAAA GAGCACCATG CAGGCAAGCC CAGACTGGAA GGAGAGGCAG 
CTCTTTGAAG AGAAGGTGGA CTTGGAGAAT GTCACAAAGA GGAAGTTTTC GCTGAAAGCC 
GCGGAGTTCG GGGAACCCAC TTCTGAGCAG ACCGGCGCGG CTGCTGGGAA ACCGGCTGCT 
CCGACTGCAA CCCCGGTGTC CTGGAAGCCG CAGGATCCCT CCGAACAGCC TCAGGAGAAG 
CGGTATCAGA GCCCGTGTGC GATGTTTGCT GCTGGAGAGA TCAAAGCCCC GGCGGTGGAG 
GGCAGCCTCG ACTCCCCCAG CAAAACCATG TCCATTAAAG AAAGATTAGC ACTGTTGAAG 
AAGAGCGGTG AGGAAGACTG GAGAAACAGA CTCAACAGAA AGCAGGAATA CGGCAAAGCC 
TCCATCACCA GCAGCCTGCA CATCCAAGAG ACGGAGCAGT CACTCAAGAA GAAGGAAGAA 
GGAGGATTTA CAGATGATAA TGACATTTCT AATCTGCTTT GGGAACCTGT ATACGCTTCT 
ACTTATTCTC CTGCTCTACC TGCTGCCCAT AAATACCTGT CTTTTGTATC TATTAATCAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATTGAAG AGAGAAAGCA TCTCATCACC 
GTGAGAGAGG ACGCCTGGAA GACCAGGGGC AAAGGAGCAG CCAACGACTC TACCCAGTTT 
ACTGTGGCCG GCAGGATGGT GAAGCGAGGT CTGGCGTCCC CCACTGCCAT CACCCCGGTA 
GCATCCCCTG TTTCCAGCAA AGCAAGGGGC ACTACACCAG TTTCCAGACC CCTGGAAGAT 
ATCGAAGCCA GACCAGACAT GCAGTTAGAG TCGGACCTCA AGCTGGACAG GCTGGAAACC 
TTCCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAAG AAACAGTCCT CACTGTCACT 
GGGAAATCGG TGAAAGAGGT GATGAAGCCG GACGATGATG AAACCTTTGC CAAGTTTTAC 
CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC CCCGTAGAGC TGGACGAGGA TTTCGATGTC 
ATTTTTGATC CTTATGCCCC CAGGTTGACA TCCTCGGTGG CTGAGCACAA GCGTGCCGTG 
CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA AACCCCCTGA AGATGCTGGC CGCGAGGGAA 
GATCTCCTGC AGGAGTACAC TGAGCAGAGA CTGAACGTGG CCTTCGTGGA GTCAAAGCGG 
ATGAAAGTCG AAAAGTTGTC CGCCAACTCC AGCTTCTCAG AAGTCACCCT GGCGGGGTTA 
GCCAGCAAAG AAAACTTCAG CAACGTCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAACA GTGCAGTGCC CTACAAGAAG CTGATGCTGC TGCAGGTTAA AGGAAGAAGA 
CACGTGCAGA CGCGGCTGGT GGAGCCTCGC GCCCCCTCCC TCAACAGCGG GGACTGCTTC 
CTCCTGCTCT CGCCCCATCA CTGCTTCCTG TGGGTCGGAG AGTTCGCCAA CGTGATCGAG 
AAGGCGAAGG CCTCAGAACT TGCGAGTTTA ATTCAGACCA AGAGGGAACT TGGTTGTAGA 
GCAACTTACA TCCAGACTGT TGAAGAAGGA ATTAATACAC ACACACATGC AGCCAAAGAC 
TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT TACCAATCCG CCGGAGACCC AAAGGAGGAT 
GAGCTCTATG AAACGGCCAT AATAGAGACC AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTCGTTCCTG ATGATGACTA CTGGGGGAAG ATCCCAAAGT GCTCCCTCCT GCAATCAAAA 
GAGGTACTGG TGTTTGATTT TGGGAGTGAA GTTTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCACAAC GGAAAATCGC CTTTCAGCTG GCAAAGCACT TGTGGAATGG AACCTTTGAC 
TACGAGAATT GTGACATTAA CCCGCTGGAT CCTGGGGAAT GCAATCCCCT CATTCCCAGG 
AAAGGACAGG GGCGGCCCGA CTGGGCAATA TTTGGGAGAC TTACAGAACA CAATGAGACG 
ATCTTGTTCA AAGAGAAATT CCTTGATTGG ACGGAACTGA AGAGACCTAA CGAGAAGAAC 
GCCAGCGAAC TCGCGCAGCA CAAGGACGAT GCCCGGGCAG AGGTCAAGCC TTACGACGTG 
ACGCGGATGG TGCCCGTGCC CCAGACAACA GCCGGCACCG TGCTGGATGG GGTCAACGTG 
GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC GACCGGAGGC AGTTCGAGAT CGCCAGCATC 
TCAGTGGACG TCTGGCACAT CCTGGAGTTC GACTACAGCA GGCTCCCCAA GCAGAGCATC 
GGGCAGTTCC ACGAAGGCGA TGCCTACGTG GTGAAGTGGA AGTTCATCGT GAGCACTGCA 
GTGGGAAGCC GGCAGAAGGG GGAGCACTCG GTCAGGGTGG CTGGCAAAGA GAAATGTGTC 
TACTTCTTCT GGCAAGGCCG CCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGACGAGGA AAGGGGGGCC CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC 
CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG 
GAGGAAGAGA ACACACAGAG CGAGTGGCGG CTGTACTGTG TGCGCGGAGA GGTGCCTGTG 
GAGGGGAACT TGCTGGAGGT GGCCTGTCAC TGCAGCAGCC TCAGGTCTAG GACGTCCATG 
GTTGTCCTCA ACGTCCACAA AGCCCTCATC TACCTGTGGC ATGGATGCAA AGCCCAGGCC 
CACACGAAGG AGGTCGGACG GACTGCAGCC AATAAAATCA AGGACCAATG TCCCCTGGAG 
GCCGGGCTGC ACAGTAGCAG CAAAGTGACA ATTCATGAGT GTGATGAAGG CTCGGAGCCC 
CTGGGGTTCT GGGACGCTCT GGGGAGGAGA GACCGGAAAG CCTACGACTG CATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCCCGC CTGTTCATCC TCAGCAGCTC CTCTGGTGAC 
TTCTCAGCCA CAGAGTTCAT GTATCCCGCC CGAGACCCCT CTGTGGTCAA CTCTATGCCC 
TTCCTGCAGG AAGACCTATA CAGCGCCCCA CAGCCAGCAC TCTTCCTTGT TGACAATCAC 
CACGAGGTGT ACCTCTGGCA AGGCTGGTGG CCCATCGAGA ACAAGATCAC GGGCTCCGCG 
CGCATCCGCT GGGCCTCGGA CCGGAAGAGC GCCATGGAGA CAGTGCTGCA GTACTGCCGA 
GGGAAAAACC TGAAGAAGCC GCCCCCCAAG TCTTACCTCA TCCACGCTGG CCTGGAACCC 
CTGACCTTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AAGACATTGC AGAGATCACG 
GAGATGGACA CGGAAGTTTC AAATCAGATC ACCCTAGTGG AGGATGTCTT AGCCAAACTG 
TGTAAGACCA TTTACCCACT GGCCGACCTC CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC 
CCTCTGAAGC TGGAGATCTA TCTCACCGAC GAGGACTTCG AGTTTGCACT AGACATGACC 
CGAGACGAGT ACAATGCACT GCCCGCTTGG AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000287.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856055.1 Bos taurus supervillin (SVIL), transcript variant X25, mRNA.

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CloneID	OBa221419
Clone ID Related Accession (Same CDS sequence)	XM_025000287.1
Accession Version	XM_025000287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGCCA TTCTCGGGTT 
GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC 
TATTTTTCTC CTGGTGAAAA TAGAAAGACT TCCGAGAGAT TTAGAACTCA ACCTATAACC 
TCGGCAGAAC GAAAGGAATC GGATAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACTGAACCT ACCCCCGCTT CGTGCTCAGT GGCCACCCAC 
CCTGTAATGA CAAGACATCC TAGCCCCACT GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA 
TTGCAGGCGT CTGCTCACCA AAAGGCACTA GCCAGAGACC AGACAAACGA GAGCAAAGAT 
TCTGCTGAGC AGGGAGAACC TGACTCCTCC ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG 
TTTAACAAAC TATCTCAGCC GGTCTCAAAA GCCATTTCAA CACGGAACAG ATTAGACATG 
AGACAGAGGA GGATGAATGC TCGTTATCAA ACCCAGCCGG TCACGCTTGG AGAAGTGGAA 
CAGGTGCAGA GTGGCAAGCT CATGGCTTTC TCTCCCACCA TTAACACTTC CGTGTCCACC 
GTGGCATCTA CCGTCCCCCC CATGTATGCA GGAAATCTTC GGACAAAGCC ACTTCCAGAT 
GACAGCTTTG GTGCCACTGA GCAGAAGTTT GCTTCTTCGC TAGAAAACTC CGACTCCCCA 
GTTAGAAGCA TCCTGAAATC CCAAGGCTGG CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA 
GCAATGTTGA GAGAATTTGA AGAGACAGAA CGCAAGGGAG GCTTGACAGG TGGAGATGGC 
GGGGTTACAA AGTATGGGTC CTTCGAGGAA GCAGAGCTGT CCTACCCCGT CCTCAGCAGA 
GTCCGGGAGG GAGACAACCA TAAAGAGGCC ATCTATGCCC TTCCGAGGAA AGGAAGCCTG 
GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT GGTGATGACC TGAAGGAATT TTCCACCCCA 
AAGAGCACCA TGCAGGCAAG CCCAGACTGG AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG 
GACTTGGAGA ATGTCACAAA GAGGAAGTTT TCGCTGAAAG CCGCGGAGTT CGGGGAACCC 
ACTTCTGAGC AGACCGGCGC GGCTGCTGGG AAACCGGCTG CTCCGACTGC AACCCCGGTG 
TCCTGGAAGC CGCAGGATCC CTCCGAACAG CCTCAGGAGA AGCGGTATCA GAGCCCGTGT 
GCGATGTTTG CTGCTGGAGA GATCAAAGCC CCGGCGGTGG AGGGCAGCCT CGACTCCCCC 
AGCAAAACCA TGTCCATTAA AGAAAGATTA GCACTGTTGA AGAAGAGCGG TGAGGAAGAC 
TGGAGAAACA GACTCAACAG AAAGCAGGAA TACGGCAAAG CCTCCATCAC CAGCAGCCTG 
CACATCCAAG AGACGGAGCA GTCACTCAAG AAGAAGCGGG TCACAGAAAG TCGAGAGAGC 
CAGATGACTA TTGAAGAGAG AAAGCATCTC ATCACCGTGA GAGAGGACGC CTGGAAGACC 
AGGGGCAAAG GAGCAGCCAA CGACTCTACC CAGTTTACTG TGGCCGGCAG GATGGTGAAG 
CGAGGTCTGG CGTCCCCCAC TGCCATCACC CCGGTAGCAT CCCCTGTTTC CAGCAAAGCA 
AGGGGCACTA CACCAGTTTC CAGACCCCTG GAAGATATCG AAGCCAGACC AGACATGCAG 
TTAGAGTCGG ACCTCAAGCT GGACAGGCTG GAAACCTTCC TAAGAAGGCT GAATAACAAA 
GTTGGTGGGA TGCAAGAAAC AGTCCTCACT GTCACTGGGA AATCGGTGAA AGAGGTGATG 
AAGCCGGACG ATGATGAAAC CTTTGCCAAG TTTTACCGCA GCGTGGACTC CTCTCTACCG 
AGAAGCCCCG TAGAGCTGGA CGAGGATTTC GATGTCATTT TTGATCCTTA TGCCCCCAGG 
TTGACATCCT CGGTGGCTGA GCACAAGCGT GCCGTGCGGC CCAAGCGCCG GGTCCAGGCT 
TCCAAAAACC CCCTGAAGAT GCTGGCCGCG AGGGAAGATC TCCTGCAGGA GTACACTGAG 
CAGAGACTGA ACGTGGCCTT CGTGGAGTCA AAGCGGATGA AAGTCGAAAA GTTGTCCGCC 
AACTCCAGCT TCTCAGAAGT CACCCTGGCG GGGTTAGCCA GCAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAATTCCA ACAACAGTGC AGTGCCCTAC 
AAGAAGCTGA TGCTGCTGCA GGTTAAAGGA AGAAGACACG TGCAGACGCG GCTGGTGGAG 
CCTCGCGCCC CCTCCCTCAA CAGCGGGGAC TGCTTCCTCC TGCTCTCGCC CCATCACTGC 
TTCCTGTGGG TCGGAGAGTT CGCCAACGTG ATCGAGAAGG CGAAGGCCTC AGAACTTGCG 
AGTTTAATTC AGACCAAGAG GGAACTTGGT TGTAGAGCAA CTTACATCCA GACTGTTGAA 
GAAGGAATTA ATACACACAC ACATGCAGCC AAAGACTTCT GGAAGCTTCT GGGCGGCCAA 
GCCAGTTACC AATCCGCCGG AGACCCAAAG GAGGATGAGC TCTATGAAAC GGCCATAATA 
GAGACCAACT GCATTTACCG TCTGATGGAT GACAAACTCG TTCCTGATGA TGACTACTGG 
GGGAAGATCC CAAAGTGCTC CCTCCTGCAA TCAAAAGAGG TACTGGTGTT TGATTTTGGG 
AGTGAAGTTT ACGTGTGGCA TGGAAAAGAA GTCACATTAG CACAACGGAA AATCGCCTTT 
CAGCTGGCAA AGCACTTGTG GAATGGAACC TTTGACTACG AGAATTGTGA CATTAACCCG 
CTGGATCCTG GGGAATGCAA TCCCCTCATT CCCAGGAAAG GACAGGGGCG GCCCGACTGG 
GCAATATTTG GGAGACTTAC AGAACACAAT GAGACGATCT TGTTCAAAGA GAAATTCCTT 
GATTGGACGG AACTGAAGAG ACCTAACGAG AAGAACGCCA GCGAACTCGC GCAGCACAAG 
GACGATGCCC GGGCAGAGGT CAAGCCTTAC GACGTGACGC GGATGGTGCC CGTGCCCCAG 
ACAACAGCCG GCACCGTGCT GGATGGGGTC AACGTGGGCC GAGGCTACGG GCTGGTGGAA 
GGGGACGACC GGAGGCAGTT CGAGATCGCC AGCATCTCAG TGGACGTCTG GCACATCCTG 
GAGTTCGACT ACAGCAGGCT CCCCAAGCAG AGCATCGGGC AGTTCCACGA AGGCGATGCC 
TACGTGGTGA AGTGGAAGTT CATCGTGAGC ACTGCAGTGG GAAGCCGGCA GAAGGGGGAG 
CACTCGGTCA GGGTGGCTGG CAAAGAGAAA TGTGTCTACT TCTTCTGGCA AGGCCGCCAG 
TCGACCGTGA GTGAGAAGGG CACATCAGCT CTGATGACAG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTCCT GCAGGGCAAG GAGCCCCCCT GTTTCCTGCA GTGCTTCCAG 
GGGGGGATGG TGGTCCACTC CGGGAGACGG GAGGAGGAGG AAGAGAACAC ACAGAGCGAG 
TGGCGGCTGT ACTGTGTGCG CGGAGAGGTG CCTGTGGAGG GGAACTTGCT GGAGGTGGCC 
TGTCACTGCA GCAGCCTCAG GTCTAGGACG TCCATGGTTG TCCTCAACGT CCACAAAGCC 
CTCATCTACC TGTGGCATGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGACGGACT 
GCAGCCAATA AAATCAAGGA CCAATGTCCC CTGGAGGCCG GGCTGCACAG TAGCAGCAAA 
GTGACAATTC ATGAGTGTGA TGAAGGCTCG GAGCCCCTGG GGTTCTGGGA CGCTCTGGGG 
AGGAGAGACC GGAAAGCCTA CGACTGCATG CTTCAAGATC CTGGAAATTT TAACTTCACA 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGTGACTTCT CAGCCACAGA GTTCATGTAT 
CCCGCCCGAG ACCCCTCTGT GGTCAACTCT ATGCCCTTCC TGCAGGAAGA CCTATACAGC 
GCCCCACAGC CAGCACTCTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACGGGC TCCGCGCGCA TCCGCTGGGC CTCGGACCGG 
AAGAGCGCCA TGGAGACAGT GCTGCAGTAC TGCCGAGGGA AAAACCTGAA GAAGCCGCCC 
CCCAAGTCTT ACCTCATCCA CGCTGGCCTG GAACCCCTGA CCTTCACCAA CATGTTCCCC 
AGCTGGGAGC ACAGAGAAGA CATTGCAGAG ATCACGGAGA TGGACACGGA AGTTTCAAAT 
CAGATCACCC TAGTGGAGGA TGTCTTAGCC AAACTGTGTA AGACCATTTA CCCACTGGCC 
GACCTCCTAG CCAGGCCGCT CCCTGAGGGA GTTGACCCTC TGAAGCTGGA GATCTATCTC 
ACCGACGAGG ACTTCGAGTT TGCACTAGAC ATGACCCGAG ACGAGTACAA TGCACTGCCC 
GCTTGGAAGC AGGTGAACCT GAAGAAAGCG AAAGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856055.1
CDS	356..6280
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASIGKPKHEWFLQKDSEGDTPSLINWPSSHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000287.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000287.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGCCA TTCTCGGGTT 
GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC 
TATTTTTCTC CTGGTGAAAA TAGAAAGACT TCCGAGAGAT TTAGAACTCA ACCTATAACC 
TCGGCAGAAC GAAAGGAATC GGATAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACTGAACCT ACCCCCGCTT CGTGCTCAGT GGCCACCCAC 
CCTGTAATGA CAAGACATCC TAGCCCCACT GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA 
TTGCAGGCGT CTGCTCACCA AAAGGCACTA GCCAGAGACC AGACAAACGA GAGCAAAGAT 
TCTGCTGAGC AGGGAGAACC TGACTCCTCC ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG 
TTTAACAAAC TATCTCAGCC GGTCTCAAAA GCCATTTCAA CACGGAACAG ATTAGACATG 
AGACAGAGGA GGATGAATGC TCGTTATCAA ACCCAGCCGG TCACGCTTGG AGAAGTGGAA 
CAGGTGCAGA GTGGCAAGCT CATGGCTTTC TCTCCCACCA TTAACACTTC CGTGTCCACC 
GTGGCATCTA CCGTCCCCCC CATGTATGCA GGAAATCTTC GGACAAAGCC ACTTCCAGAT 
GACAGCTTTG GTGCCACTGA GCAGAAGTTT GCTTCTTCGC TAGAAAACTC CGACTCCCCA 
GTTAGAAGCA TCCTGAAATC CCAAGGCTGG CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA 
GCAATGTTGA GAGAATTTGA AGAGACAGAA CGCAAGGGAG GCTTGACAGG TGGAGATGGC 
GGGGTTACAA AGTATGGGTC CTTCGAGGAA GCAGAGCTGT CCTACCCCGT CCTCAGCAGA 
GTCCGGGAGG GAGACAACCA TAAAGAGGCC ATCTATGCCC TTCCGAGGAA AGGAAGCCTG 
GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT GGTGATGACC TGAAGGAATT TTCCACCCCA 
AAGAGCACCA TGCAGGCAAG CCCAGACTGG AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG 
GACTTGGAGA ATGTCACAAA GAGGAAGTTT TCGCTGAAAG CCGCGGAGTT CGGGGAACCC 
ACTTCTGAGC AGACCGGCGC GGCTGCTGGG AAACCGGCTG CTCCGACTGC AACCCCGGTG 
TCCTGGAAGC CGCAGGATCC CTCCGAACAG CCTCAGGAGA AGCGGTATCA GAGCCCGTGT 
GCGATGTTTG CTGCTGGAGA GATCAAAGCC CCGGCGGTGG AGGGCAGCCT CGACTCCCCC 
AGCAAAACCA TGTCCATTAA AGAAAGATTA GCACTGTTGA AGAAGAGCGG TGAGGAAGAC 
TGGAGAAACA GACTCAACAG AAAGCAGGAA TACGGCAAAG CCTCCATCAC CAGCAGCCTG 
CACATCCAAG AGACGGAGCA GTCACTCAAG AAGAAGCGGG TCACAGAAAG TCGAGAGAGC 
CAGATGACTA TTGAAGAGAG AAAGCATCTC ATCACCGTGA GAGAGGACGC CTGGAAGACC 
AGGGGCAAAG GAGCAGCCAA CGACTCTACC CAGTTTACTG TGGCCGGCAG GATGGTGAAG 
CGAGGTCTGG CGTCCCCCAC TGCCATCACC CCGGTAGCAT CCCCTGTTTC CAGCAAAGCA 
AGGGGCACTA CACCAGTTTC CAGACCCCTG GAAGATATCG AAGCCAGACC AGACATGCAG 
TTAGAGTCGG ACCTCAAGCT GGACAGGCTG GAAACCTTCC TAAGAAGGCT GAATAACAAA 
GTTGGTGGGA TGCAAGAAAC AGTCCTCACT GTCACTGGGA AATCGGTGAA AGAGGTGATG 
AAGCCGGACG ATGATGAAAC CTTTGCCAAG TTTTACCGCA GCGTGGACTC CTCTCTACCG 
AGAAGCCCCG TAGAGCTGGA CGAGGATTTC GATGTCATTT TTGATCCTTA TGCCCCCAGG 
TTGACATCCT CGGTGGCTGA GCACAAGCGT GCCGTGCGGC CCAAGCGCCG GGTCCAGGCT 
TCCAAAAACC CCCTGAAGAT GCTGGCCGCG AGGGAAGATC TCCTGCAGGA GTACACTGAG 
CAGAGACTGA ACGTGGCCTT CGTGGAGTCA AAGCGGATGA AAGTCGAAAA GTTGTCCGCC 
AACTCCAGCT TCTCAGAAGT CACCCTGGCG GGGTTAGCCA GCAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAATTCCA ACAACAGTGC AGTGCCCTAC 
AAGAAGCTGA TGCTGCTGCA GGTTAAAGGA AGAAGACACG TGCAGACGCG GCTGGTGGAG 
CCTCGCGCCC CCTCCCTCAA CAGCGGGGAC TGCTTCCTCC TGCTCTCGCC CCATCACTGC 
TTCCTGTGGG TCGGAGAGTT CGCCAACGTG ATCGAGAAGG CGAAGGCCTC AGAACTTGCG 
AGTTTAATTC AGACCAAGAG GGAACTTGGT TGTAGAGCAA CTTACATCCA GACTGTTGAA 
GAAGGAATTA ATACACACAC ACATGCAGCC AAAGACTTCT GGAAGCTTCT GGGCGGCCAA 
GCCAGTTACC AATCCGCCGG AGACCCAAAG GAGGATGAGC TCTATGAAAC GGCCATAATA 
GAGACCAACT GCATTTACCG TCTGATGGAT GACAAACTCG TTCCTGATGA TGACTACTGG 
GGGAAGATCC CAAAGTGCTC CCTCCTGCAA TCAAAAGAGG TACTGGTGTT TGATTTTGGG 
AGTGAAGTTT ACGTGTGGCA TGGAAAAGAA GTCACATTAG CACAACGGAA AATCGCCTTT 
CAGCTGGCAA AGCACTTGTG GAATGGAACC TTTGACTACG AGAATTGTGA CATTAACCCG 
CTGGATCCTG GGGAATGCAA TCCCCTCATT CCCAGGAAAG GACAGGGGCG GCCCGACTGG 
GCAATATTTG GGAGACTTAC AGAACACAAT GAGACGATCT TGTTCAAAGA GAAATTCCTT 
GATTGGACGG AACTGAAGAG ACCTAACGAG AAGAACGCCA GCGAACTCGC GCAGCACAAG 
GACGATGCCC GGGCAGAGGT CAAGCCTTAC GACGTGACGC GGATGGTGCC CGTGCCCCAG 
ACAACAGCCG GCACCGTGCT GGATGGGGTC AACGTGGGCC GAGGCTACGG GCTGGTGGAA 
GGGGACGACC GGAGGCAGTT CGAGATCGCC AGCATCTCAG TGGACGTCTG GCACATCCTG 
GAGTTCGACT ACAGCAGGCT CCCCAAGCAG AGCATCGGGC AGTTCCACGA AGGCGATGCC 
TACGTGGTGA AGTGGAAGTT CATCGTGAGC ACTGCAGTGG GAAGCCGGCA GAAGGGGGAG 
CACTCGGTCA GGGTGGCTGG CAAAGAGAAA TGTGTCTACT TCTTCTGGCA AGGCCGCCAG 
TCGACCGTGA GTGAGAAGGG CACATCAGCT CTGATGACAG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTCCT GCAGGGCAAG GAGCCCCCCT GTTTCCTGCA GTGCTTCCAG 
GGGGGGATGG TGGTCCACTC CGGGAGACGG GAGGAGGAGG AAGAGAACAC ACAGAGCGAG 
TGGCGGCTGT ACTGTGTGCG CGGAGAGGTG CCTGTGGAGG GGAACTTGCT GGAGGTGGCC 
TGTCACTGCA GCAGCCTCAG GTCTAGGACG TCCATGGTTG TCCTCAACGT CCACAAAGCC 
CTCATCTACC TGTGGCATGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGACGGACT 
GCAGCCAATA AAATCAAGGA CCAATGTCCC CTGGAGGCCG GGCTGCACAG TAGCAGCAAA 
GTGACAATTC ATGAGTGTGA TGAAGGCTCG GAGCCCCTGG GGTTCTGGGA CGCTCTGGGG 
AGGAGAGACC GGAAAGCCTA CGACTGCATG CTTCAAGATC CTGGAAATTT TAACTTCACA 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGTGACTTCT CAGCCACAGA GTTCATGTAT 
CCCGCCCGAG ACCCCTCTGT GGTCAACTCT ATGCCCTTCC TGCAGGAAGA CCTATACAGC 
GCCCCACAGC CAGCACTCTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACGGGC TCCGCGCGCA TCCGCTGGGC CTCGGACCGG 
AAGAGCGCCA TGGAGACAGT GCTGCAGTAC TGCCGAGGGA AAAACCTGAA GAAGCCGCCC 
CCCAAGTCTT ACCTCATCCA CGCTGGCCTG GAACCCCTGA CCTTCACCAA CATGTTCCCC 
AGCTGGGAGC ACAGAGAAGA CATTGCAGAG ATCACGGAGA TGGACACGGA AGTTTCAAAT 
CAGATCACCC TAGTGGAGGA TGTCTTAGCC AAACTGTGTA AGACCATTTA CCCACTGGCC 
GACCTCCTAG CCAGGCCGCT CCCTGAGGGA GTTGACCCTC TGAAGCTGGA GATCTATCTC 
ACCGACGAGG ACTTCGAGTT TGCACTAGAC ATGACCCGAG ACGAGTACAA TGCACTGCCC 
GCTTGGAAGC AGGTGAACCT GAAGAAAGCG AAAGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000288.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856056.1 Bos taurus supervillin (SVIL), transcript variant X26, mRNA.

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CloneID	OBa221420
Clone ID Related Accession (Same CDS sequence)	XM_025000288.1
Accession Version	XM_025000288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGGTC AACTTCAAAT 
TCAGAAATGC CAGCTGCCGA GGATGAAGAA AAGGTCGATG AGCGAGCGAG GCTGAGCGTC 
GCTGCTAAGA GGCTGCTCTT CAGGGAGATG GAGAAGTCGT TTGACGAGAA GAGCGTCCCA 
AAGCGCCGCT CCCGCAATGC GGCCGTGGAG CAGAGGCTGC GGCGCCTCCA GGACCGGTCC 
CACACGCAGC CCGTCACCAC CGAGGAGGTG GTCATTGCAG CCACTGAACC TACCCCCGCT 
TCGTGCTCAG TGGCCACCCA CCCTGTAATG ACAAGACATC CTAGCCCCAC TGTAGCTAAG 
AGTCCCGTGC AGCCTGCCAG ATTGCAGGCG TCTGCTCACC AAAAGGCACT AGCCAGAGAC 
CAGACAAACG AGAGCAAAGA TTCTGCTGAG CAGGGAGAAC CTGACTCCTC CACTCTGAGC 
TTAGCGGAGA AGCTGGCCTT GTTTAACAAA CTATCTCAGC CGGTCTCAAA AGCCATTTCA 
ACACGGAACA GATTAGACAT GAGACAGAGG AGGATGAATG CTCGTTATCA AACCCAGCCG 
GTCACGCTTG GAGAAGTGGA ACAGGTGCAG AGTGGCAAGC TCATGGCTTT CTCTCCCACC 
ATTAACACTT CCGTGTCCAC CGTGGCATCT ACCGTCCCCC CCATGTATGC AGGAAATCTT 
CGGACAAAGC CACTTCCAGA TGACAGCTTT GGTGCCACTG AGCAGAAGTT TGCTTCTTCG 
CTAGAAAACT CCGACTCCCC AGTTAGAAGC ATCCTGAAAT CCCAAGGCTG GCAGCCCTCG 
GTCGAGGGTG CTGGGAGCAA AGCAATGTTG AGAGAATTTG AAGAGACAGA ACGCAAGGGA 
GGCTTGACAG GTGGAGATGG CGGGGTTACA AAGTATGGGT CCTTCGAGGA AGCAGAGCTG 
TCCTACCCCG TCCTCAGCAG AGTCCGGGAG GGAGACAACC ATAAAGAGGC CATCTATGCC 
CTTCCGAGGA AAGGAAGCCT GGAGCTCGCC CATCCTCCCA TCGCCCAGCT TGGTGATGAC 
CTGAAGGAAT TTTCCACCCC AAAGAGCACC ATGCAGGCAA GCCCAGACTG GAAGGAGAGG 
CAGCTCTTTG AAGAGAAGGT GGACTTGGAG AATGTCACAA AGAGGAAGTT TTCGCTGAAA 
GCCGCGGAGT TCGGGGAACC CACTTCTGAG CAGACCGGCG CGGCTGCTGG GAAACCGGCT 
GCTCCGACTG CAACCCCGGT GTCCTGGAAG CCGCAGGATC CCTCCGAACA GCCTCAGGAG 
AAGCGGTATC AGAGCCCGTG TGCGATGTTT GCTGCTGGAG AGATCAAAGC CCCGGCGGTG 
GAGGGCAGCC TCGACTCCCC CAGCAAAACC ATGTCCATTA AAGAAAGATT AGCACTGTTG 
AAGAAGAGCG GTGAGGAAGA CTGGAGAAAC AGACTCAACA GAAAGCAGGA ATACGGCAAA 
GCCTCCATCA CCAGCAGCCT GCACATCCAA GAGACGGAGC AGTCACTCAA GAAGAAGGAA 
GAAGGAGGAT TTACAGATGA TAATGACATT TCTAATCTGC TTTGGGAACC TGTATACGCT 
TCTACTTATT CTCCTGCTCT ACCTGCTGCC CATAAATACC TGTCTTTTGT ATCTATTAAT 
CAGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATTG AAGAGAGAAA GCATCTCATC 
ACCGTGAGAG AGGACGCCTG GAAGACCAGG GGCAAAGGAG CAGCCAACGA CTCTACCCAG 
TTTACTGTGG CCGGCAGGAT GGTGAAGCGA GGTCTGGCGT CCCCCACTGC CATCACCCCG 
GTAGCATCCC CTGTTTCCAG CAAAGCAAGG GGCACTACAC CAGTTTCCAG ACCCCTGGAA 
GATATCGAAG CCAGACCAGA CATGCAGTTA GAGTCGGACC TCAAGCTGGA CAGGCTGGAA 
ACCTTCCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AAGAAACAGT CCTCACTGTC 
ACTGGGAAAT CGGTGAAAGA GGTGATGAAG CCGGACGATG ATGAAACCTT TGCCAAGTTT 
TACCGCAGCG TGGACTCCTC TCTACCGAGA AGCCCCGTAG AGCTGGACGA GGATTTCGAT 
GTCATTTTTG ATCCTTATGC CCCCAGGTTG ACATCCTCGG TGGCTGAGCA CAAGCGTGCC 
GTGCGGCCCA AGCGCCGGGT CCAGGCTTCC AAAAACCCCC TGAAGATGCT GGCCGCGAGG 
GAAGATCTCC TGCAGGAGTA CACTGAGCAG AGACTGAACG TGGCCTTCGT GGAGTCAAAG 
CGGATGAAAG TCGAAAAGTT GTCCGCCAAC TCCAGCTTCT CAGAAGTCAC CCTGGCGGGG 
TTAGCCAGCA AAGAAAACTT CAGCAACGTC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ACAGTGCAGT GCCCTACAAG AAGCTGATGC TGCTGCAGGT TAAAGGAAGA 
AGACACGTGC AGACGCGGCT GGTGGAGCCT CGCGCCCCCT CCCTCAACAG CGGGGACTGC 
TTCCTCCTGC TCTCGCCCCA TCACTGCTTC CTGTGGGTCG GAGAGTTCGC CAACGTGATC 
GAGAAGGCGA AGGCCTCAGA ACTTGCGAGT TTAATTCAGA CCAAGAGGGA ACTTGGTTGT 
AGAGCAACTT ACATCCAGAC TGTTGAAGAA GGAATTAATA CACACACACA TGCAGCCAAA 
GACTTCTGGA AGCTTCTGGG CGGCCAAGCC AGTTACCAAT CCGCCGGAGA CCCAAAGGAG 
GATGAGCTCT ATGAAACGGC CATAATAGAG ACCAACTGCA TTTACCGTCT GATGGATGAC 
AAACTCGTTC CTGATGATGA CTACTGGGGG AAGATCCCAA AGTGCTCCCT CCTGCAATCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGGAGT GAAGTTTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCAC AACGGAAAAT CGCCTTTCAG CTGGCAAAGC ACTTGTGGAA TGGAACCTTT 
GACTACGAGA ATTGTGACAT TAACCCGCTG GATCCTGGGG AATGCAATCC CCTCATTCCC 
AGGAAAGGAC AGGGGCGGCC CGACTGGGCA ATATTTGGGA GACTTACAGA ACACAATGAG 
ACGATCTTGT TCAAAGAGAA ATTCCTTGAT TGGACGGAAC TGAAGAGACC TAACGAGAAG 
AACGCCAGCG AACTCGCGCA GCACAAGGAC GATGCCCGGG CAGAGGTCAA GCCTTACGAC 
GTGACGCGGA TGGTGCCCGT GCCCCAGACA ACAGCCGGCA CCGTGCTGGA TGGGGTCAAC 
GTGGGCCGAG GCTACGGGCT GGTGGAAGGG GACGACCGGA GGCAGTTCGA GATCGCCAGC 
ATCTCAGTGG ACGTCTGGCA CATCCTGGAG TTCGACTACA GCAGGCTCCC CAAGCAGAGC 
ATCGGGCAGT TCCACGAAGG CGATGCCTAC GTGGTGAAGT GGAAGTTCAT CGTGAGCACT 
GCAGTGGGAA GCCGGCAGAA GGGGGAGCAC TCGGTCAGGG TGGCTGGCAA AGAGAAATGT 
GTCTACTTCT TCTGGCAAGG CCGCCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGACGA GGAAAGGGGG GCCCAGGTCC AGGTCCTGCA GGGCAAGGAG 
CCCCCCTGTT TCCTGCAGTG CTTCCAGGGG GGGATGGTGG TCCACTCCGG GAGACGGGAG 
GAGGAGGAAG AGAACACACA GAGCGAGTGG CGGCTGTACT GTGTGCGCGG AGAGGTGCCT 
GTGGAGGGGA ACTTGCTGGA GGTGGCCTGT CACTGCAGCA GCCTCAGGTC TAGGACGTCC 
ATGGTTGTCC TCAACGTCCA CAAAGCCCTC ATCTACCTGT GGCATGGATG CAAAGCCCAG 
GCCCACACGA AGGAGGTCGG ACGGACTGCA GCCAATAAAA TCAAGGACCA ATGTCCCCTG 
GAGGCCGGGC TGCACAGTAG CAGCAAAGTG ACAATTCATG AGTGTGATGA AGGCTCGGAG 
CCCCTGGGGT TCTGGGACGC TCTGGGGAGG AGAGACCGGA AAGCCTACGA CTGCATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCC CGCCTGTTCA TCCTCAGCAG CTCCTCTGGT 
GACTTCTCAG CCACAGAGTT CATGTATCCC GCCCGAGACC CCTCTGTGGT CAACTCTATG 
CCCTTCCTGC AGGAAGACCT ATACAGCGCC CCACAGCCAG CACTCTTCCT TGTTGACAAT 
CACCACGAGG TGTACCTCTG GCAAGGCTGG TGGCCCATCG AGAACAAGAT CACGGGCTCC 
GCGCGCATCC GCTGGGCCTC GGACCGGAAG AGCGCCATGG AGACAGTGCT GCAGTACTGC 
CGAGGGAAAA ACCTGAAGAA GCCGCCCCCC AAGTCTTACC TCATCCACGC TGGCCTGGAA 
CCCCTGACCT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAAGACAT TGCAGAGATC 
ACGGAGATGG ACACGGAAGT TTCAAATCAG ATCACCCTAG TGGAGGATGT CTTAGCCAAA 
CTGTGTAAGA CCATTTACCC ACTGGCCGAC CTCCTAGCCA GGCCGCTCCC TGAGGGAGTT 
GACCCTCTGA AGCTGGAGAT CTATCTCACC GACGAGGACT TCGAGTTTGC ACTAGACATG 
ACCCGAGACG AGTACAATGC ACTGCCCGCT TGGAAGCAGG TGAACCTGAA GAAAGCGAAA 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856056.1
CDS	356..6247
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASIGKPKHEWFLQKDSEGDTPSLINWPSRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000288.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000288.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGGTC AACTTCAAAT 
TCAGAAATGC CAGCTGCCGA GGATGAAGAA AAGGTCGATG AGCGAGCGAG GCTGAGCGTC 
GCTGCTAAGA GGCTGCTCTT CAGGGAGATG GAGAAGTCGT TTGACGAGAA GAGCGTCCCA 
AAGCGCCGCT CCCGCAATGC GGCCGTGGAG CAGAGGCTGC GGCGCCTCCA GGACCGGTCC 
CACACGCAGC CCGTCACCAC CGAGGAGGTG GTCATTGCAG CCACTGAACC TACCCCCGCT 
TCGTGCTCAG TGGCCACCCA CCCTGTAATG ACAAGACATC CTAGCCCCAC TGTAGCTAAG 
AGTCCCGTGC AGCCTGCCAG ATTGCAGGCG TCTGCTCACC AAAAGGCACT AGCCAGAGAC 
CAGACAAACG AGAGCAAAGA TTCTGCTGAG CAGGGAGAAC CTGACTCCTC CACTCTGAGC 
TTAGCGGAGA AGCTGGCCTT GTTTAACAAA CTATCTCAGC CGGTCTCAAA AGCCATTTCA 
ACACGGAACA GATTAGACAT GAGACAGAGG AGGATGAATG CTCGTTATCA AACCCAGCCG 
GTCACGCTTG GAGAAGTGGA ACAGGTGCAG AGTGGCAAGC TCATGGCTTT CTCTCCCACC 
ATTAACACTT CCGTGTCCAC CGTGGCATCT ACCGTCCCCC CCATGTATGC AGGAAATCTT 
CGGACAAAGC CACTTCCAGA TGACAGCTTT GGTGCCACTG AGCAGAAGTT TGCTTCTTCG 
CTAGAAAACT CCGACTCCCC AGTTAGAAGC ATCCTGAAAT CCCAAGGCTG GCAGCCCTCG 
GTCGAGGGTG CTGGGAGCAA AGCAATGTTG AGAGAATTTG AAGAGACAGA ACGCAAGGGA 
GGCTTGACAG GTGGAGATGG CGGGGTTACA AAGTATGGGT CCTTCGAGGA AGCAGAGCTG 
TCCTACCCCG TCCTCAGCAG AGTCCGGGAG GGAGACAACC ATAAAGAGGC CATCTATGCC 
CTTCCGAGGA AAGGAAGCCT GGAGCTCGCC CATCCTCCCA TCGCCCAGCT TGGTGATGAC 
CTGAAGGAAT TTTCCACCCC AAAGAGCACC ATGCAGGCAA GCCCAGACTG GAAGGAGAGG 
CAGCTCTTTG AAGAGAAGGT GGACTTGGAG AATGTCACAA AGAGGAAGTT TTCGCTGAAA 
GCCGCGGAGT TCGGGGAACC CACTTCTGAG CAGACCGGCG CGGCTGCTGG GAAACCGGCT 
GCTCCGACTG CAACCCCGGT GTCCTGGAAG CCGCAGGATC CCTCCGAACA GCCTCAGGAG 
AAGCGGTATC AGAGCCCGTG TGCGATGTTT GCTGCTGGAG AGATCAAAGC CCCGGCGGTG 
GAGGGCAGCC TCGACTCCCC CAGCAAAACC ATGTCCATTA AAGAAAGATT AGCACTGTTG 
AAGAAGAGCG GTGAGGAAGA CTGGAGAAAC AGACTCAACA GAAAGCAGGA ATACGGCAAA 
GCCTCCATCA CCAGCAGCCT GCACATCCAA GAGACGGAGC AGTCACTCAA GAAGAAGGAA 
GAAGGAGGAT TTACAGATGA TAATGACATT TCTAATCTGC TTTGGGAACC TGTATACGCT 
TCTACTTATT CTCCTGCTCT ACCTGCTGCC CATAAATACC TGTCTTTTGT ATCTATTAAT 
CAGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATTG AAGAGAGAAA GCATCTCATC 
ACCGTGAGAG AGGACGCCTG GAAGACCAGG GGCAAAGGAG CAGCCAACGA CTCTACCCAG 
TTTACTGTGG CCGGCAGGAT GGTGAAGCGA GGTCTGGCGT CCCCCACTGC CATCACCCCG 
GTAGCATCCC CTGTTTCCAG CAAAGCAAGG GGCACTACAC CAGTTTCCAG ACCCCTGGAA 
GATATCGAAG CCAGACCAGA CATGCAGTTA GAGTCGGACC TCAAGCTGGA CAGGCTGGAA 
ACCTTCCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AAGAAACAGT CCTCACTGTC 
ACTGGGAAAT CGGTGAAAGA GGTGATGAAG CCGGACGATG ATGAAACCTT TGCCAAGTTT 
TACCGCAGCG TGGACTCCTC TCTACCGAGA AGCCCCGTAG AGCTGGACGA GGATTTCGAT 
GTCATTTTTG ATCCTTATGC CCCCAGGTTG ACATCCTCGG TGGCTGAGCA CAAGCGTGCC 
GTGCGGCCCA AGCGCCGGGT CCAGGCTTCC AAAAACCCCC TGAAGATGCT GGCCGCGAGG 
GAAGATCTCC TGCAGGAGTA CACTGAGCAG AGACTGAACG TGGCCTTCGT GGAGTCAAAG 
CGGATGAAAG TCGAAAAGTT GTCCGCCAAC TCCAGCTTCT CAGAAGTCAC CCTGGCGGGG 
TTAGCCAGCA AAGAAAACTT CAGCAACGTC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ACAGTGCAGT GCCCTACAAG AAGCTGATGC TGCTGCAGGT TAAAGGAAGA 
AGACACGTGC AGACGCGGCT GGTGGAGCCT CGCGCCCCCT CCCTCAACAG CGGGGACTGC 
TTCCTCCTGC TCTCGCCCCA TCACTGCTTC CTGTGGGTCG GAGAGTTCGC CAACGTGATC 
GAGAAGGCGA AGGCCTCAGA ACTTGCGAGT TTAATTCAGA CCAAGAGGGA ACTTGGTTGT 
AGAGCAACTT ACATCCAGAC TGTTGAAGAA GGAATTAATA CACACACACA TGCAGCCAAA 
GACTTCTGGA AGCTTCTGGG CGGCCAAGCC AGTTACCAAT CCGCCGGAGA CCCAAAGGAG 
GATGAGCTCT ATGAAACGGC CATAATAGAG ACCAACTGCA TTTACCGTCT GATGGATGAC 
AAACTCGTTC CTGATGATGA CTACTGGGGG AAGATCCCAA AGTGCTCCCT CCTGCAATCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGGAGT GAAGTTTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCAC AACGGAAAAT CGCCTTTCAG CTGGCAAAGC ACTTGTGGAA TGGAACCTTT 
GACTACGAGA ATTGTGACAT TAACCCGCTG GATCCTGGGG AATGCAATCC CCTCATTCCC 
AGGAAAGGAC AGGGGCGGCC CGACTGGGCA ATATTTGGGA GACTTACAGA ACACAATGAG 
ACGATCTTGT TCAAAGAGAA ATTCCTTGAT TGGACGGAAC TGAAGAGACC TAACGAGAAG 
AACGCCAGCG AACTCGCGCA GCACAAGGAC GATGCCCGGG CAGAGGTCAA GCCTTACGAC 
GTGACGCGGA TGGTGCCCGT GCCCCAGACA ACAGCCGGCA CCGTGCTGGA TGGGGTCAAC 
GTGGGCCGAG GCTACGGGCT GGTGGAAGGG GACGACCGGA GGCAGTTCGA GATCGCCAGC 
ATCTCAGTGG ACGTCTGGCA CATCCTGGAG TTCGACTACA GCAGGCTCCC CAAGCAGAGC 
ATCGGGCAGT TCCACGAAGG CGATGCCTAC GTGGTGAAGT GGAAGTTCAT CGTGAGCACT 
GCAGTGGGAA GCCGGCAGAA GGGGGAGCAC TCGGTCAGGG TGGCTGGCAA AGAGAAATGT 
GTCTACTTCT TCTGGCAAGG CCGCCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGACGA GGAAAGGGGG GCCCAGGTCC AGGTCCTGCA GGGCAAGGAG 
CCCCCCTGTT TCCTGCAGTG CTTCCAGGGG GGGATGGTGG TCCACTCCGG GAGACGGGAG 
GAGGAGGAAG AGAACACACA GAGCGAGTGG CGGCTGTACT GTGTGCGCGG AGAGGTGCCT 
GTGGAGGGGA ACTTGCTGGA GGTGGCCTGT CACTGCAGCA GCCTCAGGTC TAGGACGTCC 
ATGGTTGTCC TCAACGTCCA CAAAGCCCTC ATCTACCTGT GGCATGGATG CAAAGCCCAG 
GCCCACACGA AGGAGGTCGG ACGGACTGCA GCCAATAAAA TCAAGGACCA ATGTCCCCTG 
GAGGCCGGGC TGCACAGTAG CAGCAAAGTG ACAATTCATG AGTGTGATGA AGGCTCGGAG 
CCCCTGGGGT TCTGGGACGC TCTGGGGAGG AGAGACCGGA AAGCCTACGA CTGCATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCC CGCCTGTTCA TCCTCAGCAG CTCCTCTGGT 
GACTTCTCAG CCACAGAGTT CATGTATCCC GCCCGAGACC CCTCTGTGGT CAACTCTATG 
CCCTTCCTGC AGGAAGACCT ATACAGCGCC CCACAGCCAG CACTCTTCCT TGTTGACAAT 
CACCACGAGG TGTACCTCTG GCAAGGCTGG TGGCCCATCG AGAACAAGAT CACGGGCTCC 
GCGCGCATCC GCTGGGCCTC GGACCGGAAG AGCGCCATGG AGACAGTGCT GCAGTACTGC 
CGAGGGAAAA ACCTGAAGAA GCCGCCCCCC AAGTCTTACC TCATCCACGC TGGCCTGGAA 
CCCCTGACCT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAAGACAT TGCAGAGATC 
ACGGAGATGG ACACGGAAGT TTCAAATCAG ATCACCCTAG TGGAGGATGT CTTAGCCAAA 
CTGTGTAAGA CCATTTACCC ACTGGCCGAC CTCCTAGCCA GGCCGCTCCC TGAGGGAGTT 
GACCCTCTGA AGCTGGAGAT CTATCTCACC GACGAGGACT TCGAGTTTGC ACTAGACATG 
ACCCGAGACG AGTACAATGC ACTGCCCGCT TGGAAGCAGG TGAACCTGAA GAAAGCGAAA 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000289.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856057.1 Bos taurus supervillin (SVIL), transcript variant X27, mRNA.

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CloneID	OBa221421
Clone ID Related Accession (Same CDS sequence)	XM_025000289.1
Accession Version	XM_025000289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACTGAACCT ACCCCCGCTT CGTGCTCAGT GGCCACCCAC 
CCTGTAATGA CAAGACATCC TAGCCCCACT GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA 
TTGCAGGCGT CTGCTCACCA AAAGGCACTA GCCAGAGACC AGACAAACGA GAGCAAAGAT 
TCTGCTGAGC AGGGAGAACC TGACTCCTCC ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG 
TTTAACAAAC TATCTCAGCC GGTCTCAAAA GCCATTTCAA CACGGAACAG ATTAGACATG 
AGACAGAGGA GGATGAATGC TCGTTATCAA ACCCAGCCGG TCACGCTTGG AGAAGTGGAA 
CAGGTGCAGA GTGGCAAGCT CATGGCTTTC TCTCCCACCA TTAACACTTC CGTGTCCACC 
GTGGCATCTA CCGTCCCCCC CATGTATGCA GGAAATCTTC GGACAAAGCC ACTTCCAGAT 
GACAGCTTTG GTGCCACTGA GCAGAAGTTT GCTTCTTCGC TAGAAAACTC CGACTCCCCA 
GTTAGAAGCA TCCTGAAATC CCAAGGCTGG CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA 
GCAATGTTGA GAGAATTTGA AGAGACAGAA CGCAAGGGAG GCTTGACAGG TGGAGATGGC 
GGGGTTACAA AGTATGGGTC CTTCGAGGAA GCAGAGCTGT CCTACCCCGT CCTCAGCAGA 
GTCCGGGAGG GAGACAACCA TAAAGAGGCC ATCTATGCCC TTCCGAGGAA AGGAAGCCTG 
GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT GGTGATGACC TGAAGGAATT TTCCACCCCA 
AAGAGCACCA TGCAGGCAAG CCCAGACTGG AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG 
GACTTGGAGA ATGTCACAAA GAGGAAGTTT TCGCTGAAAG CCGCGGAGTT CGGGGAACCC 
ACTTCTGAGC AGACCGGCGC GGCTGCTGGG AAACCGGCTG CTCCGACTGC AACCCCGGTG 
TCCTGGAAGC CGCAGGATCC CTCCGAACAG CCTCAGGAGA AGCGGTATCA GAGCCCGTGT 
GCGATGTTTG CTGCTGGAGA GATCAAAGCC CCGGCGGTGG AGGGCAGCCT CGACTCCCCC 
AGCAAAACCA TGTCCATTAA AGAAAGATTA GCACTGTTGA AGAAGAGCGG TGAGGAAGAC 
TGGAGAAACA GACTCAACAG AAAGCAGGAA TACGGCAAAG CCTCCATCAC CAGCAGCCTG 
CACATCCAAG AGACGGAGCA GTCACTCAAG AAGAAGGAAG AAGGAGGATT TACAGATGAT 
AATGACATTT CTAATCTGCT TTGGGAACCT GTATACGCTT CTACTTATTC TCCTGCTCTA 
CCTGCTGCCC ATAAATACCT GTCTTTTGTA TCTATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAGA TGACTATTGA AGAGAGAAAG CATCTCATCA CCGTGAGAGA GGACGCCTGG 
AAGACCAGGG GCAAAGGAGC AGCCAACGAC TCTACCCAGT TTACTGTGGC CGGCAGGATG 
GTGAAGCGAG GTCTGGCGTC CCCCACTGCC ATCACCCCGG TAGCATCCCC TGTTTCCAGC 
AAAGCAAGGG GCACTACACC AGTTTCCAGA CCCCTGGAAG ATATCGAAGC CAGACCAGAC 
ATGCAGTTAG AGTCGGACCT CAAGCTGGAC AGGCTGGAAA CCTTCCTAAG AAGGCTGAAT 
AACAAAGTTG GTGGGATGCA AGAAACAGTC CTCACTGTCA CTGGGAAATC GGTGAAAGAG 
GTGATGAAGC CGGACGATGA TGAAACCTTT GCCAAGTTTT ACCGCAGCGT GGACTCCTCT 
CTACCGAGAA GCCCCGTAGA GCTGGACGAG GATTTCGATG TCATTTTTGA TCCTTATGCC 
CCCAGGTTGA CATCCTCGGT GGCTGAGCAC AAGCGTGCCG TGCGGCCCAA GCGCCGGGTC 
CAGGCTTCCA AAAACCCCCT GAAGATGCTG GCCGCGAGGG AAGATCTCCT GCAGGAGTAC 
ACTGAGCAGA GACTGAACGT GGCCTTCGTG GAGTCAAAGC GGATGAAAGT CGAAAAGTTG 
TCCGCCAACT CCAGCTTCTC AGAAGTCACC CTGGCGGGGT TAGCCAGCAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ATTCCAACAA CAGTGCAGTG 
CCCTACAAGA AGCTGATGCT GCTGCAGGTT AAAGGAAGAA GACACGTGCA GACGCGGCTG 
GTGGAGCCTC GCGCCCCCTC CCTCAACAGC GGGGACTGCT TCCTCCTGCT CTCGCCCCAT 
CACTGCTTCC TGTGGGTCGG AGAGTTCGCC AACGTGATCG AGAAGGCGAA GGCCTCAGAA 
CTTGCGAGTT TAATTCAGAC CAAGAGGGAA CTTGGTTGTA GAGCAACTTA CATCCAGACT 
GTTGAAGAAG GAATTAATAC ACACACACAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGC 
GGCCAAGCCA GTTACCAATC CGCCGGAGAC CCAAAGGAGG ATGAGCTCTA TGAAACGGCC 
ATAATAGAGA CCAACTGCAT TTACCGTCTG ATGGATGACA AACTCGTTCC TGATGATGAC 
TACTGGGGGA AGATCCCAAA GTGCTCCCTC CTGCAATCAA AAGAGGTACT GGTGTTTGAT 
TTTGGGAGTG AAGTTTACGT GTGGCATGGA AAAGAAGTCA CATTAGCACA ACGGAAAATC 
GCCTTTCAGC TGGCAAAGCA CTTGTGGAAT GGAACCTTTG ACTACGAGAA TTGTGACATT 
AACCCGCTGG ATCCTGGGGA ATGCAATCCC CTCATTCCCA GGAAAGGACA GGGGCGGCCC 
GACTGGGCAA TATTTGGGAG ACTTACAGAA CACAATGAGA CGATCTTGTT CAAAGAGAAA 
TTCCTTGATT GGACGGAACT GAAGAGACCT AACGAGAAGA ACGCCAGCGA ACTCGCGCAG 
CACAAGGACG ATGCCCGGGC AGAGGTCAAG CCTTACGACG TGACGCGGAT GGTGCCCGTG 
CCCCAGACAA CAGCCGGCAC CGTGCTGGAT GGGGTCAACG TGGGCCGAGG CTACGGGCTG 
GTGGAAGGGG ACGACCGGAG GCAGTTCGAG ATCGCCAGCA TCTCAGTGGA CGTCTGGCAC 
ATCCTGGAGT TCGACTACAG CAGGCTCCCC AAGCAGAGCA TCGGGCAGTT CCACGAAGGC 
GATGCCTACG TGGTGAAGTG GAAGTTCATC GTGAGCACTG CAGTGGGAAG CCGGCAGAAG 
GGGGAGCACT CGGTCAGGGT GGCTGGCAAA GAGAAATGTG TCTACTTCTT CTGGCAAGGC 
CGCCAGTCGA CCGTGAGTGA GAAGGGCACA TCAGCTCTGA TGACAGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTCCTGCAG GGCAAGGAGC CCCCCTGTTT CCTGCAGTGC 
TTCCAGGGGG GGATGGTGGT CCACTCCGGG AGACGGGAGG AGGAGGAAGA GAACACACAG 
AGCGAGTGGC GGCTGTACTG TGTGCGCGGA GAGGTGCCTG TGGAGGGGAA CTTGCTGGAG 
GTGGCCTGTC ACTGCAGCAG CCTCAGGTCT AGGACGTCCA TGGTTGTCCT CAACGTCCAC 
AAAGCCCTCA TCTACCTGTG GCATGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
CGGACTGCAG CCAATAAAAT CAAGGACCAA TGTCCCCTGG AGGCCGGGCT GCACAGTAGC 
AGCAAAGTGA CAATTCATGA GTGTGATGAA GGCTCGGAGC CCCTGGGGTT CTGGGACGCT 
CTGGGGAGGA GAGACCGGAA AGCCTACGAC TGCATGCTTC AAGATCCTGG AAATTTTAAC 
TTCACACCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGTG ACTTCTCAGC CACAGAGTTC 
ATGTATCCCG CCCGAGACCC CTCTGTGGTC AACTCTATGC CCTTCCTGCA GGAAGACCTA 
TACAGCGCCC CACAGCCAGC ACTCTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACGGGCTCCG CGCGCATCCG CTGGGCCTCG 
GACCGGAAGA GCGCCATGGA GACAGTGCTG CAGTACTGCC GAGGGAAAAA CCTGAAGAAG 
CCGCCCCCCA AGTCTTACCT CATCCACGCT GGCCTGGAAC CCCTGACCTT CACCAACATG 
TTCCCCAGCT GGGAGCACAG AGAAGACATT GCAGAGATCA CGGAGATGGA CACGGAAGTT 
TCAAATCAGA TCACCCTAGT GGAGGATGTC TTAGCCAAAC TGTGTAAGAC CATTTACCCA 
CTGGCCGACC TCCTAGCCAG GCCGCTCCCT GAGGGAGTTG ACCCTCTGAA GCTGGAGATC 
TATCTCACCG ACGAGGACTT CGAGTTTGCA CTAGACATGA CCCGAGACGA GTACAATGCA 
CTGCCCGCTT GGAAGCAGGT GAACCTGAAG AAAGCGAAAG GTCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856057.1
CDS	356..6166
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARARSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000289.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000289.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACTGAACCT ACCCCCGCTT CGTGCTCAGT GGCCACCCAC 
CCTGTAATGA CAAGACATCC TAGCCCCACT GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA 
TTGCAGGCGT CTGCTCACCA AAAGGCACTA GCCAGAGACC AGACAAACGA GAGCAAAGAT 
TCTGCTGAGC AGGGAGAACC TGACTCCTCC ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG 
TTTAACAAAC TATCTCAGCC GGTCTCAAAA GCCATTTCAA CACGGAACAG ATTAGACATG 
AGACAGAGGA GGATGAATGC TCGTTATCAA ACCCAGCCGG TCACGCTTGG AGAAGTGGAA 
CAGGTGCAGA GTGGCAAGCT CATGGCTTTC TCTCCCACCA TTAACACTTC CGTGTCCACC 
GTGGCATCTA CCGTCCCCCC CATGTATGCA GGAAATCTTC GGACAAAGCC ACTTCCAGAT 
GACAGCTTTG GTGCCACTGA GCAGAAGTTT GCTTCTTCGC TAGAAAACTC CGACTCCCCA 
GTTAGAAGCA TCCTGAAATC CCAAGGCTGG CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA 
GCAATGTTGA GAGAATTTGA AGAGACAGAA CGCAAGGGAG GCTTGACAGG TGGAGATGGC 
GGGGTTACAA AGTATGGGTC CTTCGAGGAA GCAGAGCTGT CCTACCCCGT CCTCAGCAGA 
GTCCGGGAGG GAGACAACCA TAAAGAGGCC ATCTATGCCC TTCCGAGGAA AGGAAGCCTG 
GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT GGTGATGACC TGAAGGAATT TTCCACCCCA 
AAGAGCACCA TGCAGGCAAG CCCAGACTGG AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG 
GACTTGGAGA ATGTCACAAA GAGGAAGTTT TCGCTGAAAG CCGCGGAGTT CGGGGAACCC 
ACTTCTGAGC AGACCGGCGC GGCTGCTGGG AAACCGGCTG CTCCGACTGC AACCCCGGTG 
TCCTGGAAGC CGCAGGATCC CTCCGAACAG CCTCAGGAGA AGCGGTATCA GAGCCCGTGT 
GCGATGTTTG CTGCTGGAGA GATCAAAGCC CCGGCGGTGG AGGGCAGCCT CGACTCCCCC 
AGCAAAACCA TGTCCATTAA AGAAAGATTA GCACTGTTGA AGAAGAGCGG TGAGGAAGAC 
TGGAGAAACA GACTCAACAG AAAGCAGGAA TACGGCAAAG CCTCCATCAC CAGCAGCCTG 
CACATCCAAG AGACGGAGCA GTCACTCAAG AAGAAGGAAG AAGGAGGATT TACAGATGAT 
AATGACATTT CTAATCTGCT TTGGGAACCT GTATACGCTT CTACTTATTC TCCTGCTCTA 
CCTGCTGCCC ATAAATACCT GTCTTTTGTA TCTATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAGA TGACTATTGA AGAGAGAAAG CATCTCATCA CCGTGAGAGA GGACGCCTGG 
AAGACCAGGG GCAAAGGAGC AGCCAACGAC TCTACCCAGT TTACTGTGGC CGGCAGGATG 
GTGAAGCGAG GTCTGGCGTC CCCCACTGCC ATCACCCCGG TAGCATCCCC TGTTTCCAGC 
AAAGCAAGGG GCACTACACC AGTTTCCAGA CCCCTGGAAG ATATCGAAGC CAGACCAGAC 
ATGCAGTTAG AGTCGGACCT CAAGCTGGAC AGGCTGGAAA CCTTCCTAAG AAGGCTGAAT 
AACAAAGTTG GTGGGATGCA AGAAACAGTC CTCACTGTCA CTGGGAAATC GGTGAAAGAG 
GTGATGAAGC CGGACGATGA TGAAACCTTT GCCAAGTTTT ACCGCAGCGT GGACTCCTCT 
CTACCGAGAA GCCCCGTAGA GCTGGACGAG GATTTCGATG TCATTTTTGA TCCTTATGCC 
CCCAGGTTGA CATCCTCGGT GGCTGAGCAC AAGCGTGCCG TGCGGCCCAA GCGCCGGGTC 
CAGGCTTCCA AAAACCCCCT GAAGATGCTG GCCGCGAGGG AAGATCTCCT GCAGGAGTAC 
ACTGAGCAGA GACTGAACGT GGCCTTCGTG GAGTCAAAGC GGATGAAAGT CGAAAAGTTG 
TCCGCCAACT CCAGCTTCTC AGAAGTCACC CTGGCGGGGT TAGCCAGCAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ATTCCAACAA CAGTGCAGTG 
CCCTACAAGA AGCTGATGCT GCTGCAGGTT AAAGGAAGAA GACACGTGCA GACGCGGCTG 
GTGGAGCCTC GCGCCCCCTC CCTCAACAGC GGGGACTGCT TCCTCCTGCT CTCGCCCCAT 
CACTGCTTCC TGTGGGTCGG AGAGTTCGCC AACGTGATCG AGAAGGCGAA GGCCTCAGAA 
CTTGCGAGTT TAATTCAGAC CAAGAGGGAA CTTGGTTGTA GAGCAACTTA CATCCAGACT 
GTTGAAGAAG GAATTAATAC ACACACACAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGC 
GGCCAAGCCA GTTACCAATC CGCCGGAGAC CCAAAGGAGG ATGAGCTCTA TGAAACGGCC 
ATAATAGAGA CCAACTGCAT TTACCGTCTG ATGGATGACA AACTCGTTCC TGATGATGAC 
TACTGGGGGA AGATCCCAAA GTGCTCCCTC CTGCAATCAA AAGAGGTACT GGTGTTTGAT 
TTTGGGAGTG AAGTTTACGT GTGGCATGGA AAAGAAGTCA CATTAGCACA ACGGAAAATC 
GCCTTTCAGC TGGCAAAGCA CTTGTGGAAT GGAACCTTTG ACTACGAGAA TTGTGACATT 
AACCCGCTGG ATCCTGGGGA ATGCAATCCC CTCATTCCCA GGAAAGGACA GGGGCGGCCC 
GACTGGGCAA TATTTGGGAG ACTTACAGAA CACAATGAGA CGATCTTGTT CAAAGAGAAA 
TTCCTTGATT GGACGGAACT GAAGAGACCT AACGAGAAGA ACGCCAGCGA ACTCGCGCAG 
CACAAGGACG ATGCCCGGGC AGAGGTCAAG CCTTACGACG TGACGCGGAT GGTGCCCGTG 
CCCCAGACAA CAGCCGGCAC CGTGCTGGAT GGGGTCAACG TGGGCCGAGG CTACGGGCTG 
GTGGAAGGGG ACGACCGGAG GCAGTTCGAG ATCGCCAGCA TCTCAGTGGA CGTCTGGCAC 
ATCCTGGAGT TCGACTACAG CAGGCTCCCC AAGCAGAGCA TCGGGCAGTT CCACGAAGGC 
GATGCCTACG TGGTGAAGTG GAAGTTCATC GTGAGCACTG CAGTGGGAAG CCGGCAGAAG 
GGGGAGCACT CGGTCAGGGT GGCTGGCAAA GAGAAATGTG TCTACTTCTT CTGGCAAGGC 
CGCCAGTCGA CCGTGAGTGA GAAGGGCACA TCAGCTCTGA TGACAGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTCCTGCAG GGCAAGGAGC CCCCCTGTTT CCTGCAGTGC 
TTCCAGGGGG GGATGGTGGT CCACTCCGGG AGACGGGAGG AGGAGGAAGA GAACACACAG 
AGCGAGTGGC GGCTGTACTG TGTGCGCGGA GAGGTGCCTG TGGAGGGGAA CTTGCTGGAG 
GTGGCCTGTC ACTGCAGCAG CCTCAGGTCT AGGACGTCCA TGGTTGTCCT CAACGTCCAC 
AAAGCCCTCA TCTACCTGTG GCATGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
CGGACTGCAG CCAATAAAAT CAAGGACCAA TGTCCCCTGG AGGCCGGGCT GCACAGTAGC 
AGCAAAGTGA CAATTCATGA GTGTGATGAA GGCTCGGAGC CCCTGGGGTT CTGGGACGCT 
CTGGGGAGGA GAGACCGGAA AGCCTACGAC TGCATGCTTC AAGATCCTGG AAATTTTAAC 
TTCACACCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGTG ACTTCTCAGC CACAGAGTTC 
ATGTATCCCG CCCGAGACCC CTCTGTGGTC AACTCTATGC CCTTCCTGCA GGAAGACCTA 
TACAGCGCCC CACAGCCAGC ACTCTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACGGGCTCCG CGCGCATCCG CTGGGCCTCG 
GACCGGAAGA GCGCCATGGA GACAGTGCTG CAGTACTGCC GAGGGAAAAA CCTGAAGAAG 
CCGCCCCCCA AGTCTTACCT CATCCACGCT GGCCTGGAAC CCCTGACCTT CACCAACATG 
TTCCCCAGCT GGGAGCACAG AGAAGACATT GCAGAGATCA CGGAGATGGA CACGGAAGTT 
TCAAATCAGA TCACCCTAGT GGAGGATGTC TTAGCCAAAC TGTGTAAGAC CATTTACCCA 
CTGGCCGACC TCCTAGCCAG GCCGCTCCCT GAGGGAGTTG ACCCTCTGAA GCTGGAGATC 
TATCTCACCG ACGAGGACTT CGAGTTTGCA CTAGACATGA CCCGAGACGA GTACAATGCA 
CTGCCCGCTT GGAAGCAGGT GAACCTGAAG AAAGCGAAAG GTCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000290.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856058.1 Bos taurus supervillin (SVIL), transcript variant X28, mRNA.

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CloneID	OBa221422
Clone ID Related Accession (Same CDS sequence)	XM_025000290.1
Accession Version	XM_025000290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACTGAACCT ACCCCCGCTT CGTGCTCAGT GGCCACCCAC 
CCTGTAATGA CAAGACATCC TAGCCCCACT GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA 
TTGCAGGCGT CTGCTCACCA AAAGGCACTA GCCAGAGACC AGACAAACGA GAGCAAAGAT 
TCTGCTGAGC AGGGAGAACC TGACTCCTCC ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG 
TTTAACAAAC TATCTCAGCC GGTCTCAAAA GCCATTTCAA CACGGAACAG ATTAGACATG 
AGACAGAGGA GGATGAATGC TCGTTATCAA ACCCAGCCGG TCACGCTTGG AGAAGTGGAA 
CAGGTGCAGA GTGGCAAGCT CATGGCTTTC TCTCCCACCA TTAACACTTC CGTGTCCACC 
GTGGCATCTA CCGTCCCCCC CATGTATGCA GGAAATCTTC GGACAAAGCC ACTTCCAGAT 
GACAGCTTTG GTGCCACTGA GCAGAAGTTT GCTTCTTCGC TAGAAAACTC CGACTCCCCA 
GTTAGAAGCA TCCTGAAATC CCAAGGCTGG CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA 
GCAATGTTGA GAGAATTTGA AGAGACAGAA CGCAAGGGAG GCTTGACAGG TGGAGATGGC 
GGGGTTACAA AGTATGGGTC CTTCGAGGAA GCAGAGCTGT CCTACCCCGT CCTCAGCAGA 
GTCCGGGAGG GAGACAACCA TAAAGAGGCC ATCTATGCCC TTCCGAGGAA AGGAAGCCTG 
GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT GGTGATGACC TGAAGGAATT TTCCACCCCA 
AAGAGCACCA TGCAGGCAAG CCCAGACTGG AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG 
GACTTGGAGA ATGTCACAAA GAGGAAGTTT TCGCTGAAAG CCGCGGAGTT CGGGGAACCC 
ACTTCTGAGC AGACCGGCGC GGCTGCTGGG AAACCGGCTG CTCCGACTGC AACCCCGGTG 
TCCTGGAAGC CGCAGGATCC CTCCGAACAG CCTCAGGAGA AGCGGTATCA GAGCCCGTGT 
GCGATGTTTG CTGCTGGAGA GATCAAAGCC CCGGCGGTGG AGGGCAGCCT CGACTCCCCC 
AGCAAAACCA TGTCCATTAA AGAAAGATTA GCACTGTTGA AGAAGAGCGG TGAGGAAGAC 
TGGAGAAACA GACTCAACAG AAAGCAGGAA TACGGCAAAG CCTCCATCAC CAGCAGCCTG 
CACATCCAAG AGACGGAGCA GTCACTCAAG AAGAAGCGGG TCACAGAAAG TCGAGAGAGC 
CAGATGACTA TTGAAGAGAG AAAGCATCTC ATCACCGTGA GAGAGGACGC CTGGAAGACC 
AGGGGCAAAG GAGCAGCCAA CGACTCTACC CAGTTTACTG TGGCCGGCAG GATGGTGAAG 
CGAGGTCTGG CGTCCCCCAC TGCCATCACC CCGGTAGCAT CCCCTGTTTC CAGCAAAGCA 
AGGGGCACTA CACCAGTTTC CAGACCCCTG GAAGATATCG AAGCCAGACC AGACATGCAG 
TTAGAGTCGG ACCTCAAGCT GGACAGGCTG GAAACCTTCC TAAGAAGGCT GAATAACAAA 
GTTGGTGGGA TGCAAGAAAC AGTCCTCACT GTCACTGGGA AATCGGTGAA AGAGGTGATG 
AAGCCGGACG ATGATGAAAC CTTTGCCAAG TTTTACCGCA GCGTGGACTC CTCTCTACCG 
AGAAGCCCCG TAGAGCTGGA CGAGGATTTC GATGTCATTT TTGATCCTTA TGCCCCCAGG 
TTGACATCCT CGGTGGCTGA GCACAAGCGT GCCGTGCGGC CCAAGCGCCG GGTCCAGGCT 
TCCAAAAACC CCCTGAAGAT GCTGGCCGCG AGGGAAGATC TCCTGCAGGA GTACACTGAG 
CAGAGACTGA ACGTGGCCTT CGTGGAGTCA AAGCGGATGA AAGTCGAAAA GTTGTCCGCC 
AACTCCAGCT TCTCAGAAGT CACCCTGGCG GGGTTAGCCA GCAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAATTCCA ACAACAGTGC AGTGCCCTAC 
AAGAAGCTGA TGCTGCTGCA GGTTAAAGGA AGAAGACACG TGCAGACGCG GCTGGTGGAG 
CCTCGCGCCC CCTCCCTCAA CAGCGGGGAC TGCTTCCTCC TGCTCTCGCC CCATCACTGC 
TTCCTGTGGG TCGGAGAGTT CGCCAACGTG ATCGAGAAGG CGAAGGCCTC AGAACTTGCG 
AGTTTAATTC AGACCAAGAG GGAACTTGGT TGTAGAGCAA CTTACATCCA GACTGTTGAA 
GAAGGAATTA ATACACACAC ACATGCAGCC AAAGACTTCT GGAAGCTTCT GGGCGGCCAA 
GCCAGTTACC AATCCGCCGG AGACCCAAAG GAGGATGAGC TCTATGAAAC GGCCATAATA 
GAGACCAACT GCATTTACCG TCTGATGGAT GACAAACTCG TTCCTGATGA TGACTACTGG 
GGGAAGATCC CAAAGTGCTC CCTCCTGCAA TCAAAAGAGG TACTGGTGTT TGATTTTGGG 
AGTGAAGTTT ACGTGTGGCA TGGAAAAGAA GTCACATTAG CACAACGGAA AATCGCCTTT 
CAGCTGGCAA AGCACTTGTG GAATGGAACC TTTGACTACG AGAATTGTGA CATTAACCCG 
CTGGATCCTG GGGAATGCAA TCCCCTCATT CCCAGGAAAG GACAGGGGCG GCCCGACTGG 
GCAATATTTG GGAGACTTAC AGAACACAAT GAGACGATCT TGTTCAAAGA GAAATTCCTT 
GATTGGACGG AACTGAAGAG ACCTAACGAG AAGAACGCCA GCGAACTCGC GCAGCACAAG 
GACGATGCCC GGGCAGAGGT CAAGCCTTAC GACGTGACGC GGATGGTGCC CGTGCCCCAG 
ACAACAGCCG GCACCGTGCT GGATGGGGTC AACGTGGGCC GAGGCTACGG GCTGGTGGAA 
GGGGACGACC GGAGGCAGTT CGAGATCGCC AGCATCTCAG TGGACGTCTG GCACATCCTG 
GAGTTCGACT ACAGCAGGCT CCCCAAGCAG AGCATCGGGC AGTTCCACGA AGGCGATGCC 
TACGTGGTGA AGTGGAAGTT CATCGTGAGC ACTGCAGTGG GAAGCCGGCA GAAGGGGGAG 
CACTCGGTCA GGGTGGCTGG CAAAGAGAAA TGTGTCTACT TCTTCTGGCA AGGCCGCCAG 
TCGACCGTGA GTGAGAAGGG CACATCAGCT CTGATGACAG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTCCT GCAGGGCAAG GAGCCCCCCT GTTTCCTGCA GTGCTTCCAG 
GGGGGGATGG TGGTCCACTC CGGGAGACGG GAGGAGGAGG AAGAGAACAC ACAGAGCGAG 
TGGCGGCTGT ACTGTGTGCG CGGAGAGGTG CCTGTGGAGG GGAACTTGCT GGAGGTGGCC 
TGTCACTGCA GCAGCCTCAG GTCTAGGACG TCCATGGTTG TCCTCAACGT CCACAAAGCC 
CTCATCTACC TGTGGCATGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGACGGACT 
GCAGCCAATA AAATCAAGGA CCAATGTCCC CTGGAGGCCG GGCTGCACAG TAGCAGCAAA 
GTGACAATTC ATGAGTGTGA TGAAGGCTCG GAGCCCCTGG GGTTCTGGGA CGCTCTGGGG 
AGGAGAGACC GGAAAGCCTA CGACTGCATG CTTCAAGATC CTGGAAATTT TAACTTCACA 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGTGACTTCT CAGCCACAGA GTTCATGTAT 
CCCGCCCGAG ACCCCTCTGT GGTCAACTCT ATGCCCTTCC TGCAGGAAGA CCTATACAGC 
GCCCCACAGC CAGCACTCTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACGGGC TCCGCGCGCA TCCGCTGGGC CTCGGACCGG 
AAGAGCGCCA TGGAGACAGT GCTGCAGTAC TGCCGAGGGA AAAACCTGAA GAAGCCGCCC 
CCCAAGTCTT ACCTCATCCA CGCTGGCCTG GAACCCCTGA CCTTCACCAA CATGTTCCCC 
AGCTGGGAGC ACAGAGAAGA CATTGCAGAG ATCACGGAGA TGGACACGGA AGTTTCAAAT 
CAGATCACCC TAGTGGAGGA TGTCTTAGCC AAACTGTGTA AGACCATTTA CCCACTGGCC 
GACCTCCTAG CCAGGCCGCT CCCTGAGGGA GTTGACCCTC TGAAGCTGGA GATCTATCTC 
ACCGACGAGG ACTTCGAGTT TGCACTAGAC ATGACCCGAG ACGAGTACAA TGCACTGCCC 
GCTTGGAAGC AGGTGAACCT GAAGAAAGCG AAAGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856058.1
CDS	356..6040
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARARSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000290.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000290.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACTGAACCT ACCCCCGCTT CGTGCTCAGT GGCCACCCAC 
CCTGTAATGA CAAGACATCC TAGCCCCACT GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA 
TTGCAGGCGT CTGCTCACCA AAAGGCACTA GCCAGAGACC AGACAAACGA GAGCAAAGAT 
TCTGCTGAGC AGGGAGAACC TGACTCCTCC ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG 
TTTAACAAAC TATCTCAGCC GGTCTCAAAA GCCATTTCAA CACGGAACAG ATTAGACATG 
AGACAGAGGA GGATGAATGC TCGTTATCAA ACCCAGCCGG TCACGCTTGG AGAAGTGGAA 
CAGGTGCAGA GTGGCAAGCT CATGGCTTTC TCTCCCACCA TTAACACTTC CGTGTCCACC 
GTGGCATCTA CCGTCCCCCC CATGTATGCA GGAAATCTTC GGACAAAGCC ACTTCCAGAT 
GACAGCTTTG GTGCCACTGA GCAGAAGTTT GCTTCTTCGC TAGAAAACTC CGACTCCCCA 
GTTAGAAGCA TCCTGAAATC CCAAGGCTGG CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA 
GCAATGTTGA GAGAATTTGA AGAGACAGAA CGCAAGGGAG GCTTGACAGG TGGAGATGGC 
GGGGTTACAA AGTATGGGTC CTTCGAGGAA GCAGAGCTGT CCTACCCCGT CCTCAGCAGA 
GTCCGGGAGG GAGACAACCA TAAAGAGGCC ATCTATGCCC TTCCGAGGAA AGGAAGCCTG 
GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT GGTGATGACC TGAAGGAATT TTCCACCCCA 
AAGAGCACCA TGCAGGCAAG CCCAGACTGG AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG 
GACTTGGAGA ATGTCACAAA GAGGAAGTTT TCGCTGAAAG CCGCGGAGTT CGGGGAACCC 
ACTTCTGAGC AGACCGGCGC GGCTGCTGGG AAACCGGCTG CTCCGACTGC AACCCCGGTG 
TCCTGGAAGC CGCAGGATCC CTCCGAACAG CCTCAGGAGA AGCGGTATCA GAGCCCGTGT 
GCGATGTTTG CTGCTGGAGA GATCAAAGCC CCGGCGGTGG AGGGCAGCCT CGACTCCCCC 
AGCAAAACCA TGTCCATTAA AGAAAGATTA GCACTGTTGA AGAAGAGCGG TGAGGAAGAC 
TGGAGAAACA GACTCAACAG AAAGCAGGAA TACGGCAAAG CCTCCATCAC CAGCAGCCTG 
CACATCCAAG AGACGGAGCA GTCACTCAAG AAGAAGCGGG TCACAGAAAG TCGAGAGAGC 
CAGATGACTA TTGAAGAGAG AAAGCATCTC ATCACCGTGA GAGAGGACGC CTGGAAGACC 
AGGGGCAAAG GAGCAGCCAA CGACTCTACC CAGTTTACTG TGGCCGGCAG GATGGTGAAG 
CGAGGTCTGG CGTCCCCCAC TGCCATCACC CCGGTAGCAT CCCCTGTTTC CAGCAAAGCA 
AGGGGCACTA CACCAGTTTC CAGACCCCTG GAAGATATCG AAGCCAGACC AGACATGCAG 
TTAGAGTCGG ACCTCAAGCT GGACAGGCTG GAAACCTTCC TAAGAAGGCT GAATAACAAA 
GTTGGTGGGA TGCAAGAAAC AGTCCTCACT GTCACTGGGA AATCGGTGAA AGAGGTGATG 
AAGCCGGACG ATGATGAAAC CTTTGCCAAG TTTTACCGCA GCGTGGACTC CTCTCTACCG 
AGAAGCCCCG TAGAGCTGGA CGAGGATTTC GATGTCATTT TTGATCCTTA TGCCCCCAGG 
TTGACATCCT CGGTGGCTGA GCACAAGCGT GCCGTGCGGC CCAAGCGCCG GGTCCAGGCT 
TCCAAAAACC CCCTGAAGAT GCTGGCCGCG AGGGAAGATC TCCTGCAGGA GTACACTGAG 
CAGAGACTGA ACGTGGCCTT CGTGGAGTCA AAGCGGATGA AAGTCGAAAA GTTGTCCGCC 
AACTCCAGCT TCTCAGAAGT CACCCTGGCG GGGTTAGCCA GCAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAATTCCA ACAACAGTGC AGTGCCCTAC 
AAGAAGCTGA TGCTGCTGCA GGTTAAAGGA AGAAGACACG TGCAGACGCG GCTGGTGGAG 
CCTCGCGCCC CCTCCCTCAA CAGCGGGGAC TGCTTCCTCC TGCTCTCGCC CCATCACTGC 
TTCCTGTGGG TCGGAGAGTT CGCCAACGTG ATCGAGAAGG CGAAGGCCTC AGAACTTGCG 
AGTTTAATTC AGACCAAGAG GGAACTTGGT TGTAGAGCAA CTTACATCCA GACTGTTGAA 
GAAGGAATTA ATACACACAC ACATGCAGCC AAAGACTTCT GGAAGCTTCT GGGCGGCCAA 
GCCAGTTACC AATCCGCCGG AGACCCAAAG GAGGATGAGC TCTATGAAAC GGCCATAATA 
GAGACCAACT GCATTTACCG TCTGATGGAT GACAAACTCG TTCCTGATGA TGACTACTGG 
GGGAAGATCC CAAAGTGCTC CCTCCTGCAA TCAAAAGAGG TACTGGTGTT TGATTTTGGG 
AGTGAAGTTT ACGTGTGGCA TGGAAAAGAA GTCACATTAG CACAACGGAA AATCGCCTTT 
CAGCTGGCAA AGCACTTGTG GAATGGAACC TTTGACTACG AGAATTGTGA CATTAACCCG 
CTGGATCCTG GGGAATGCAA TCCCCTCATT CCCAGGAAAG GACAGGGGCG GCCCGACTGG 
GCAATATTTG GGAGACTTAC AGAACACAAT GAGACGATCT TGTTCAAAGA GAAATTCCTT 
GATTGGACGG AACTGAAGAG ACCTAACGAG AAGAACGCCA GCGAACTCGC GCAGCACAAG 
GACGATGCCC GGGCAGAGGT CAAGCCTTAC GACGTGACGC GGATGGTGCC CGTGCCCCAG 
ACAACAGCCG GCACCGTGCT GGATGGGGTC AACGTGGGCC GAGGCTACGG GCTGGTGGAA 
GGGGACGACC GGAGGCAGTT CGAGATCGCC AGCATCTCAG TGGACGTCTG GCACATCCTG 
GAGTTCGACT ACAGCAGGCT CCCCAAGCAG AGCATCGGGC AGTTCCACGA AGGCGATGCC 
TACGTGGTGA AGTGGAAGTT CATCGTGAGC ACTGCAGTGG GAAGCCGGCA GAAGGGGGAG 
CACTCGGTCA GGGTGGCTGG CAAAGAGAAA TGTGTCTACT TCTTCTGGCA AGGCCGCCAG 
TCGACCGTGA GTGAGAAGGG CACATCAGCT CTGATGACAG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTCCT GCAGGGCAAG GAGCCCCCCT GTTTCCTGCA GTGCTTCCAG 
GGGGGGATGG TGGTCCACTC CGGGAGACGG GAGGAGGAGG AAGAGAACAC ACAGAGCGAG 
TGGCGGCTGT ACTGTGTGCG CGGAGAGGTG CCTGTGGAGG GGAACTTGCT GGAGGTGGCC 
TGTCACTGCA GCAGCCTCAG GTCTAGGACG TCCATGGTTG TCCTCAACGT CCACAAAGCC 
CTCATCTACC TGTGGCATGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGACGGACT 
GCAGCCAATA AAATCAAGGA CCAATGTCCC CTGGAGGCCG GGCTGCACAG TAGCAGCAAA 
GTGACAATTC ATGAGTGTGA TGAAGGCTCG GAGCCCCTGG GGTTCTGGGA CGCTCTGGGG 
AGGAGAGACC GGAAAGCCTA CGACTGCATG CTTCAAGATC CTGGAAATTT TAACTTCACA 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGTGACTTCT CAGCCACAGA GTTCATGTAT 
CCCGCCCGAG ACCCCTCTGT GGTCAACTCT ATGCCCTTCC TGCAGGAAGA CCTATACAGC 
GCCCCACAGC CAGCACTCTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACGGGC TCCGCGCGCA TCCGCTGGGC CTCGGACCGG 
AAGAGCGCCA TGGAGACAGT GCTGCAGTAC TGCCGAGGGA AAAACCTGAA GAAGCCGCCC 
CCCAAGTCTT ACCTCATCCA CGCTGGCCTG GAACCCCTGA CCTTCACCAA CATGTTCCCC 
AGCTGGGAGC ACAGAGAAGA CATTGCAGAG ATCACGGAGA TGGACACGGA AGTTTCAAAT 
CAGATCACCC TAGTGGAGGA TGTCTTAGCC AAACTGTGTA AGACCATTTA CCCACTGGCC 
GACCTCCTAG CCAGGCCGCT CCCTGAGGGA GTTGACCCTC TGAAGCTGGA GATCTATCTC 
ACCGACGAGG ACTTCGAGTT TGCACTAGAC ATGACCCGAG ACGAGTACAA TGCACTGCCC 
GCTTGGAAGC AGGTGAACCT GAAGAAAGCG AAAGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000291.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856059.1 Bos taurus supervillin (SVIL), transcript variant X29, mRNA.

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CloneID	OBa221423
Clone ID Related Accession (Same CDS sequence)	XM_025000291.1
Accession Version	XM_025000291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACATTGCAG GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA 
GACCAGACAA ACGAGAGCAA AGATTCTGCT GAGCAGGGAG AACCTGACTC CTCCACTCTG 
AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC AAACTATCTC AGCCGGTCTC AAAAGCCATT 
TCAACACGGA ACAGATTAGA CATGAGACAG AGGAGGATGA ATGCTCGTTA TCAAACCCAG 
CCGGTCACGC TTGGAGAAGT GGAACAGGTG CAGAGTGGCA AGCTCATGGC TTTCTCTCCC 
ACCATTAACA CTTCCGTGTC CACCGTGGCA TCTACCGTCC CCCCCATGTA TGCAGGAAAT 
CTTCGGACAA AGCCACTTCC AGATGACAGC TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT 
TCGCTAGAAA ACTCCGACTC CCCAGTTAGA AGCATCCTGA AATCCCAAGG CTGGCAGCCC 
TCGGTCGAGG GTGCTGGGAG CAAAGCAATG TTGAGAGAAT TTGAAGAGAC AGAACGCAAG 
GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT ACAAAGTATG GGTCCTTCGA GGAAGCAGAG 
CTGTCCTACC CCGTCCTCAG CAGAGTCCGG GAGGGAGACA ACCATAAAGA GGCCATCTAT 
GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC GCCCATCCTC CCATCGCCCA GCTTGGTGAT 
GACCTGAAGG AATTTTCCAC CCCAAAGAGC ACCATGCAGG CAAGCCCAGA CTGGAAGGAG 
AGGCAGCTCT TTGAAGAGAA GGTGGACTTG GAGAATGTCA CAAAGAGGAA GTTTTCGCTG 
AAAGCCGCGG AGTTCGGGGA ACCCACTTCT GAGCAGACCG GCGCGGCTGC TGGGAAACCG 
GCTGCTCCGA CTGCAACCCC GGTGTCCTGG AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG 
GAGAAGCGGT ATCAGAGCCC GTGTGCGATG TTTGCTGCTG GAGAGATCAA AGCCCCGGCG 
GTGGAGGGCA GCCTCGACTC CCCCAGCAAA ACCATGTCCA TTAAAGAAAG ATTAGCACTG 
TTGAAGAAGA GCGGTGAGGA AGACTGGAGA AACAGACTCA ACAGAAAGCA GGAATACGGC 
AAAGCCTCCA TCACCAGCAG CCTGCACATC CAAGAGACGG AGCAGTCACT CAAGAAGAAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATTGAAG AGAGAAAGCA TCTCATCACC 
GTGAGAGAGG ACGCCTGGAA GACCAGGGGC AAAGGAGCAG CCAACGACTC TACCCAGTTT 
ACTGTGGCCG GCAGGATGGT GAAGCGAGGT CTGGCGTCCC CCACTGCCAT CACCCCGGTA 
GCATCCCCTG TTTCCAGCAA AGCAAGGGGC ACTACACCAG TTTCCAGACC CCTGGAAGAT 
ATCGAAGCCA GACCAGACAT GCAGTTAGAG TCGGACCTCA AGCTGGACAG GCTGGAAACC 
TTCCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAAG AAACAGTCCT CACTGTCACT 
GGGAAATCGG TGAAAGAGGT GATGAAGCCG GACGATGATG AAACCTTTGC CAAGTTTTAC 
CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC CCCGTAGAGC TGGACGAGGA TTTCGATGTC 
ATTTTTGATC CTTATGCCCC CAGGTTGACA TCCTCGGTGG CTGAGCACAA GCGTGCCGTG 
CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA AACCCCCTGA AGATGCTGGC CGCGAGGGAA 
GATCTCCTGC AGGAGTACAC TGAGCAGAGA CTGAACGTGG CCTTCGTGGA GTCAAAGCGG 
ATGAAAGTCG AAAAGTTGTC CGCCAACTCC AGCTTCTCAG AAGTCACCCT GGCGGGGTTA 
GCCAGCAAAG AAAACTTCAG CAACGTCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAACA GTGCAGTGCC CTACAAGAAG CTGATGCTGC TGCAGGTTAA AGGAAGAAGA 
CACGTGCAGA CGCGGCTGGT GGAGCCTCGC GCCCCCTCCC TCAACAGCGG GGACTGCTTC 
CTCCTGCTCT CGCCCCATCA CTGCTTCCTG TGGGTCGGAG AGTTCGCCAA CGTGATCGAG 
AAGGCGAAGG CCTCAGAACT TGCGAGTTTA ATTCAGACCA AGAGGGAACT TGGTTGTAGA 
GCAACTTACA TCCAGACTGT TGAAGAAGGA ATTAATACAC ACACACATGC AGCCAAAGAC 
TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT TACCAATCCG CCGGAGACCC AAAGGAGGAT 
GAGCTCTATG AAACGGCCAT AATAGAGACC AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTCGTTCCTG ATGATGACTA CTGGGGGAAG ATCCCAAAGT GCTCCCTCCT GCAATCAAAA 
GAGGTACTGG TGTTTGATTT TGGGAGTGAA GTTTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCACAAC GGAAAATCGC CTTTCAGCTG GCAAAGCACT TGTGGAATGG AACCTTTGAC 
TACGAGAATT GTGACATTAA CCCGCTGGAT CCTGGGGAAT GCAATCCCCT CATTCCCAGG 
AAAGGACAGG GGCGGCCCGA CTGGGCAATA TTTGGGAGAC TTACAGAACA CAATGAGACG 
ATCTTGTTCA AAGAGAAATT CCTTGATTGG ACGGAACTGA AGAGACCTAA CGAGAAGAAC 
GCCAGCGAAC TCGCGCAGCA CAAGGACGAT GCCCGGGCAG AGGTCAAGCC TTACGACGTG 
ACGCGGATGG TGCCCGTGCC CCAGACAACA GCCGGCACCG TGCTGGATGG GGTCAACGTG 
GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC GACCGGAGGC AGTTCGAGAT CGCCAGCATC 
TCAGTGGACG TCTGGCACAT CCTGGAGTTC GACTACAGCA GGCTCCCCAA GCAGAGCATC 
GGGCAGTTCC ACGAAGGCGA TGCCTACGTG GTGAAGTGGA AGTTCATCGT GAGCACTGCA 
GTGGGAAGCC GGCAGAAGGG GGAGCACTCG GTCAGGGTGG CTGGCAAAGA GAAATGTGTC 
TACTTCTTCT GGCAAGGCCG CCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGACGAGGA AAGGGGGGCC CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC 
CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG 
GAGGAAGAGA ACACACAGAG CGAGTGGCGG CTGTACTGTG TGCGCGGAGA GGTGCCTGTG 
GAGGGGAACT TGCTGGAGGT GGCCTGTCAC TGCAGCAGCC TCAGGTCTAG GACGTCCATG 
GTTGTCCTCA ACGTCCACAA AGCCCTCATC TACCTGTGGC ATGGATGCAA AGCCCAGGCC 
CACACGAAGG AGGTCGGACG GACTGCAGCC AATAAAATCA AGGACCAATG TCCCCTGGAG 
GCCGGGCTGC ACAGTAGCAG CAAAGTGACA ATTCATGAGT GTGATGAAGG CTCGGAGCCC 
CTGGGGTTCT GGGACGCTCT GGGGAGGAGA GACCGGAAAG CCTACGACTG CATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCCCGC CTGTTCATCC TCAGCAGCTC CTCTGGTGAC 
TTCTCAGCCA CAGAGTTCAT GTATCCCGCC CGAGACCCCT CTGTGGTCAA CTCTATGCCC 
TTCCTGCAGG AAGACCTATA CAGCGCCCCA CAGCCAGCAC TCTTCCTTGT TGACAATCAC 
CACGAGGTGT ACCTCTGGCA AGGCTGGTGG CCCATCGAGA ACAAGATCAC GGGCTCCGCG 
CGCATCCGCT GGGCCTCGGA CCGGAAGAGC GCCATGGAGA CAGTGCTGCA GTACTGCCGA 
GGGAAAAACC TGAAGAAGCC GCCCCCCAAG TCTTACCTCA TCCACGCTGG CCTGGAACCC 
CTGACCTTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AAGACATTGC AGAGATCACG 
GAGATGGACA CGGAAGTTTC AAATCAGATC ACCCTAGTGG AGGATGTCTT AGCCAAACTG 
TGTAAGACCA TTTACCCACT GGCCGACCTC CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC 
CCTCTGAAGC TGGAGATCTA TCTCACCGAC GAGGACTTCG AGTTTGCACT AGACATGACC 
CGAGACGAGT ACAATGCACT GCCCGCTTGG AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856059.1
CDS	356..5944
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARARSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000291.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000291.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACATTGCAG GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA 
GACCAGACAA ACGAGAGCAA AGATTCTGCT GAGCAGGGAG AACCTGACTC CTCCACTCTG 
AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC AAACTATCTC AGCCGGTCTC AAAAGCCATT 
TCAACACGGA ACAGATTAGA CATGAGACAG AGGAGGATGA ATGCTCGTTA TCAAACCCAG 
CCGGTCACGC TTGGAGAAGT GGAACAGGTG CAGAGTGGCA AGCTCATGGC TTTCTCTCCC 
ACCATTAACA CTTCCGTGTC CACCGTGGCA TCTACCGTCC CCCCCATGTA TGCAGGAAAT 
CTTCGGACAA AGCCACTTCC AGATGACAGC TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT 
TCGCTAGAAA ACTCCGACTC CCCAGTTAGA AGCATCCTGA AATCCCAAGG CTGGCAGCCC 
TCGGTCGAGG GTGCTGGGAG CAAAGCAATG TTGAGAGAAT TTGAAGAGAC AGAACGCAAG 
GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT ACAAAGTATG GGTCCTTCGA GGAAGCAGAG 
CTGTCCTACC CCGTCCTCAG CAGAGTCCGG GAGGGAGACA ACCATAAAGA GGCCATCTAT 
GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC GCCCATCCTC CCATCGCCCA GCTTGGTGAT 
GACCTGAAGG AATTTTCCAC CCCAAAGAGC ACCATGCAGG CAAGCCCAGA CTGGAAGGAG 
AGGCAGCTCT TTGAAGAGAA GGTGGACTTG GAGAATGTCA CAAAGAGGAA GTTTTCGCTG 
AAAGCCGCGG AGTTCGGGGA ACCCACTTCT GAGCAGACCG GCGCGGCTGC TGGGAAACCG 
GCTGCTCCGA CTGCAACCCC GGTGTCCTGG AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG 
GAGAAGCGGT ATCAGAGCCC GTGTGCGATG TTTGCTGCTG GAGAGATCAA AGCCCCGGCG 
GTGGAGGGCA GCCTCGACTC CCCCAGCAAA ACCATGTCCA TTAAAGAAAG ATTAGCACTG 
TTGAAGAAGA GCGGTGAGGA AGACTGGAGA AACAGACTCA ACAGAAAGCA GGAATACGGC 
AAAGCCTCCA TCACCAGCAG CCTGCACATC CAAGAGACGG AGCAGTCACT CAAGAAGAAG 
CGGGTCACAG AAAGTCGAGA GAGCCAGATG ACTATTGAAG AGAGAAAGCA TCTCATCACC 
GTGAGAGAGG ACGCCTGGAA GACCAGGGGC AAAGGAGCAG CCAACGACTC TACCCAGTTT 
ACTGTGGCCG GCAGGATGGT GAAGCGAGGT CTGGCGTCCC CCACTGCCAT CACCCCGGTA 
GCATCCCCTG TTTCCAGCAA AGCAAGGGGC ACTACACCAG TTTCCAGACC CCTGGAAGAT 
ATCGAAGCCA GACCAGACAT GCAGTTAGAG TCGGACCTCA AGCTGGACAG GCTGGAAACC 
TTCCTAAGAA GGCTGAATAA CAAAGTTGGT GGGATGCAAG AAACAGTCCT CACTGTCACT 
GGGAAATCGG TGAAAGAGGT GATGAAGCCG GACGATGATG AAACCTTTGC CAAGTTTTAC 
CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC CCCGTAGAGC TGGACGAGGA TTTCGATGTC 
ATTTTTGATC CTTATGCCCC CAGGTTGACA TCCTCGGTGG CTGAGCACAA GCGTGCCGTG 
CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA AACCCCCTGA AGATGCTGGC CGCGAGGGAA 
GATCTCCTGC AGGAGTACAC TGAGCAGAGA CTGAACGTGG CCTTCGTGGA GTCAAAGCGG 
ATGAAAGTCG AAAAGTTGTC CGCCAACTCC AGCTTCTCAG AAGTCACCCT GGCGGGGTTA 
GCCAGCAAAG AAAACTTCAG CAACGTCAGC CTGCGGAGCG TCAACCTGAC GGAACAGAAT 
TCCAACAACA GTGCAGTGCC CTACAAGAAG CTGATGCTGC TGCAGGTTAA AGGAAGAAGA 
CACGTGCAGA CGCGGCTGGT GGAGCCTCGC GCCCCCTCCC TCAACAGCGG GGACTGCTTC 
CTCCTGCTCT CGCCCCATCA CTGCTTCCTG TGGGTCGGAG AGTTCGCCAA CGTGATCGAG 
AAGGCGAAGG CCTCAGAACT TGCGAGTTTA ATTCAGACCA AGAGGGAACT TGGTTGTAGA 
GCAACTTACA TCCAGACTGT TGAAGAAGGA ATTAATACAC ACACACATGC AGCCAAAGAC 
TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT TACCAATCCG CCGGAGACCC AAAGGAGGAT 
GAGCTCTATG AAACGGCCAT AATAGAGACC AACTGCATTT ACCGTCTGAT GGATGACAAA 
CTCGTTCCTG ATGATGACTA CTGGGGGAAG ATCCCAAAGT GCTCCCTCCT GCAATCAAAA 
GAGGTACTGG TGTTTGATTT TGGGAGTGAA GTTTACGTGT GGCATGGAAA AGAAGTCACA 
TTAGCACAAC GGAAAATCGC CTTTCAGCTG GCAAAGCACT TGTGGAATGG AACCTTTGAC 
TACGAGAATT GTGACATTAA CCCGCTGGAT CCTGGGGAAT GCAATCCCCT CATTCCCAGG 
AAAGGACAGG GGCGGCCCGA CTGGGCAATA TTTGGGAGAC TTACAGAACA CAATGAGACG 
ATCTTGTTCA AAGAGAAATT CCTTGATTGG ACGGAACTGA AGAGACCTAA CGAGAAGAAC 
GCCAGCGAAC TCGCGCAGCA CAAGGACGAT GCCCGGGCAG AGGTCAAGCC TTACGACGTG 
ACGCGGATGG TGCCCGTGCC CCAGACAACA GCCGGCACCG TGCTGGATGG GGTCAACGTG 
GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC GACCGGAGGC AGTTCGAGAT CGCCAGCATC 
TCAGTGGACG TCTGGCACAT CCTGGAGTTC GACTACAGCA GGCTCCCCAA GCAGAGCATC 
GGGCAGTTCC ACGAAGGCGA TGCCTACGTG GTGAAGTGGA AGTTCATCGT GAGCACTGCA 
GTGGGAAGCC GGCAGAAGGG GGAGCACTCG GTCAGGGTGG CTGGCAAAGA GAAATGTGTC 
TACTTCTTCT GGCAAGGCCG CCAGTCGACC GTGAGTGAGA AGGGCACATC AGCTCTGATG 
ACAGTGGAGC TGGACGAGGA AAGGGGGGCC CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC 
CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG 
GAGGAAGAGA ACACACAGAG CGAGTGGCGG CTGTACTGTG TGCGCGGAGA GGTGCCTGTG 
GAGGGGAACT TGCTGGAGGT GGCCTGTCAC TGCAGCAGCC TCAGGTCTAG GACGTCCATG 
GTTGTCCTCA ACGTCCACAA AGCCCTCATC TACCTGTGGC ATGGATGCAA AGCCCAGGCC 
CACACGAAGG AGGTCGGACG GACTGCAGCC AATAAAATCA AGGACCAATG TCCCCTGGAG 
GCCGGGCTGC ACAGTAGCAG CAAAGTGACA ATTCATGAGT GTGATGAAGG CTCGGAGCCC 
CTGGGGTTCT GGGACGCTCT GGGGAGGAGA GACCGGAAAG CCTACGACTG CATGCTTCAA 
GATCCTGGAA ATTTTAACTT CACACCCCGC CTGTTCATCC TCAGCAGCTC CTCTGGTGAC 
TTCTCAGCCA CAGAGTTCAT GTATCCCGCC CGAGACCCCT CTGTGGTCAA CTCTATGCCC 
TTCCTGCAGG AAGACCTATA CAGCGCCCCA CAGCCAGCAC TCTTCCTTGT TGACAATCAC 
CACGAGGTGT ACCTCTGGCA AGGCTGGTGG CCCATCGAGA ACAAGATCAC GGGCTCCGCG 
CGCATCCGCT GGGCCTCGGA CCGGAAGAGC GCCATGGAGA CAGTGCTGCA GTACTGCCGA 
GGGAAAAACC TGAAGAAGCC GCCCCCCAAG TCTTACCTCA TCCACGCTGG CCTGGAACCC 
CTGACCTTCA CCAACATGTT CCCCAGCTGG GAGCACAGAG AAGACATTGC AGAGATCACG 
GAGATGGACA CGGAAGTTTC AAATCAGATC ACCCTAGTGG AGGATGTCTT AGCCAAACTG 
TGTAAGACCA TTTACCCACT GGCCGACCTC CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC 
CCTCTGAAGC TGGAGATCTA TCTCACCGAC GAGGACTTCG AGTTTGCACT AGACATGACC 
CGAGACGAGT ACAATGCACT GCCCGCTTGG AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT 
CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000292.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856060.1 Bos taurus supervillin (SVIL), transcript variant X30, mRNA.

Price & Availability↑

CloneID	OBa221424
Clone ID Related Accession (Same CDS sequence)	XM_025000292.1 , XM_025000293.1
Accession Version	XM_025000292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGCGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856060.1
CDS	721..6099
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARARSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000292.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000292.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGCGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000293.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856061.1 Bos taurus supervillin (SVIL), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OBa221424
Clone ID Related Accession (Same CDS sequence)	XM_025000292.1 , XM_025000293.1
Accession Version	XM_025000293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGCGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856061.1
CDS	369..5747
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARARSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000293.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000293.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGCGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( NM_174190.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776615.1 Bos taurus supervillin (SVIL), mRNA.

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CloneID	OBa43786
Clone ID Related Accession (Same CDS sequence)	NM_174190.2
Accession Version	NM_174190.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Bos taurus(cattle)
Product	supervillin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF025996.1. On or before Oct 4, 2005 this sequence version replaced XM_865705.1, NM_174190.1. ##Evidence-Data-START## Transcript exon combination :: AF025996.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGTGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776615.1
CDS	200..5578
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARARSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	NM_174190.2
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174190.2

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGTGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000263.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856031.1 Bos taurus supervillin (SVIL), transcript variant X1, mRNA.

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CloneID	OBa221404
Clone ID Related Accession (Same CDS sequence)	XM_025000263.1
Accession Version	XM_025000263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTCCATTC TCGGGTTGAG GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG 
GAGAGAGGGT CCCGGAAACC GAGGCGCTAT TTTTCTCCTG GTGAAAATAG AAAGACTTCC 
GAGAGATTTA GAACTCAACC TATAACCTCG GCAGAACGAA AGGAATCGGA TAGGTCAACT 
TCAAATTCAG AAATGCCAGC TGCCGAGGAT GAAGAAAAGG TCGATGAGCG AGCGAGGCTG 
AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG GAGATGGAGA AGTCGTTTGA CGAGAAGAGC 
GTCCCAAAGC GCCGCTCCCG CAATGCGGCC GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC 
CGGTCCCACA CGCAGCCCGT CACCACCGAG GAGGTGGTCA TTGCAGCCAC TGAACCTACC 
CCCGCTTCGT GCTCAGTGGC CACCCACCCT GTAATGACAA GACATCCTAG CCCCACTGTA 
GCTAAGAGTC CCGTGCAGCC TGCCAGATTG CAGGCGTCTG CTCACCAAAA GGCACTAGCC 
AGAGACCAGA CAAACGAGAG CAAAGATTCT GCTGAGCAGG GAGAACCTGA CTCCTCCACT 
CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT AACAAACTAT CTCAGCCGGT CTCAAAAGCC 
ATTTCAACAC GGAACAGATT AGACATGAGA CAGAGGAGGA TGAATGCTCG TTATCAAACC 
CAGCCGGTCA CGCTTGGAGA AGTGGAACAG GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT 
CCCACCATTA ACACTTCCGT GTCCACCGTG GCATCTACCG TCCCCCCCAT GTATGCAGGA 
AATCTTCGGA CAAAGCCACT TCCAGATGAC AGCTTTGGTG CCACTGAGCA GAAGTTTGCT 
TCTTCGCTAG AAAACTCCGA CTCCCCAGTT AGAAGCATCC TGAAATCCCA AGGCTGGCAG 
CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA ATGTTGAGAG AATTTGAAGA GACAGAACGC 
AAGGGAGGCT TGACAGGTGG AGATGGCGGG GTTACAAAGT ATGGGTCCTT CGAGGAAGCA 
GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC CGGGAGGGAG ACAACCATAA AGAGGCCATC 
TATGCCCTTC CGAGGAAAGG AAGCCTGGAG CTCGCCCATC CTCCCATCGC CCAGCTTGGT 
GATGACCTGA AGGAATTTTC CACCCCAAAG AGCACCATGC AGGCAAGCCC AGACTGGAAG 
GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC TTGGAGAATG TCACAAAGAG GAAGTTTTCG 
CTGAAAGCCG CGGAGTTCGG GGAACCCACT TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA 
CCGGCTGCTC CGACTGCAAC CCCGGTGTCC TGGAAGCCGC AGGATCCCTC CGAACAGCCT 
CAGGAGAAGC GGTATCAGAG CCCGTGTGCG ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG 
GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC AAAACCATGT CCATTAAAGA AAGATTAGCA 
CTGTTGAAGA AGAGCGGTGA GGAAGACTGG AGAAACAGAC TCAACAGAAA GCAGGAATAC 
GGCAAAGCCT CCATCACCAG CAGCCTGCAC ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG 
AAGGAAGAAG GAGGATTTAC AGATGATAAT GACATTTCTA ATCTGCTTTG GGAACCTGTA 
TACGCTTCTA CTTATTCTCC TGCTCTACCT GCTGCCCATA AATACCTGTC TTTTGTATCT 
ATTAATCAGC GGGTCACAGA AAGTCGAGAG AGCCAGATGA CTATTGAAGA GAGAAAGCAT 
CTCATCACCG TGAGAGAGGA CGCCTGGAAG ACCAGGGGCA AAGGAGCAGC CAACGACTCT 
ACCCAGTTTA CTGTGGCCGG CAGGATGGTG AAGCGAGGTC TGGCGTCCCC CACTGCCATC 
ACCCCGGTAG CATCCCCTGT TTCCAGCAAA GCAAGGGGCA CTACACCAGT TTCCAGACCC 
CTGGAAGATA TCGAAGCCAG ACCAGACATG CAGTTAGAGT CGGACCTCAA GCTGGACAGG 
CTGGAAACCT TCCTAAGAAG GCTGAATAAC AAAGTTGGTG GGATGCAAGA AACAGTCCTC 
ACTGTCACTG GGAAATCGGT GAAAGAGGTG ATGAAGCCGG ACGATGATGA AACCTTTGCC 
AAGTTTTACC GCAGCGTGGA CTCCTCTCTA CCGAGAAGCC CCGTAGAGCT GGACGAGGAT 
TTCGATGTCA TTTTTGATCC TTATGCCCCC AGGTTGACAT CCTCGGTGGC TGAGCACAAG 
CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG GCTTCCAAAA ACCCCCTGAA GATGCTGGCC 
GCGAGGGAAG ATCTCCTGCA GGAGTACACT GAGCAGAGAC TGAACGTGGC CTTCGTGGAG 
TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC GCCAACTCCA GCTTCTCAGA AGTCACCCTG 
GCGGGGTTAG CCAGCAAAGA AAACTTCAGC AACGTCAGCC TGCGGAGCGT CAACCTGACG 
GAACAGAATT CCAACAACAG TGCAGTGCCC TACAAGAAGC TGATGCTGCT GCAGGTTAAA 
GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG GAGCCTCGCG CCCCCTCCCT CAACAGCGGG 
GACTGCTTCC TCCTGCTCTC GCCCCATCAC TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC 
GTGATCGAGA AGGCGAAGGC CTCAGAACTT GCGAGTTTAA TTCAGACCAA GAGGGAACTT 
GGTTGTAGAG CAACTTACAT CCAGACTGTT GAAGAAGGAA TTAATACACA CACACATGCA 
GCCAAAGACT TCTGGAAGCT TCTGGGCGGC CAAGCCAGTT ACCAATCCGC CGGAGACCCA 
AAGGAGGATG AGCTCTATGA AACGGCCATA ATAGAGACCA ACTGCATTTA CCGTCTGATG 
GATGACAAAC TCGTTCCTGA TGATGACTAC TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG 
CAATCAAAAG AGGTACTGGT GTTTGATTTT GGGAGTGAAG TTTACGTGTG GCATGGAAAA 
GAAGTCACAT TAGCACAACG GAAAATCGCC TTTCAGCTGG CAAAGCACTT GTGGAATGGA 
ACCTTTGACT ACGAGAATTG TGACATTAAC CCGCTGGATC CTGGGGAATG CAATCCCCTC 
ATTCCCAGGA AAGGACAGGG GCGGCCCGAC TGGGCAATAT TTGGGAGACT TACAGAACAC 
AATGAGACGA TCTTGTTCAA AGAGAAATTC CTTGATTGGA CGGAACTGAA GAGACCTAAC 
GAGAAGAACG CCAGCGAACT CGCGCAGCAC AAGGACGATG CCCGGGCAGA GGTCAAGCCT 
TACGACGTGA CGCGGATGGT GCCCGTGCCC CAGACAACAG CCGGCACCGT GCTGGATGGG 
GTCAACGTGG GCCGAGGCTA CGGGCTGGTG GAAGGGGACG ACCGGAGGCA GTTCGAGATC 
GCCAGCATCT CAGTGGACGT CTGGCACATC CTGGAGTTCG ACTACAGCAG GCTCCCCAAG 
CAGAGCATCG GGCAGTTCCA CGAAGGCGAT GCCTACGTGG TGAAGTGGAA GTTCATCGTG 
AGCACTGCAG TGGGAAGCCG GCAGAAGGGG GAGCACTCGG TCAGGGTGGC TGGCAAAGAG 
AAATGTGTCT ACTTCTTCTG GCAAGGCCGC CAGTCGACCG TGAGTGAGAA GGGCACATCA 
GCTCTGATGA CAGTGGAGCT GGACGAGGAA AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC 
AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA 
CGGGAGGAGG AGGAAGAGAA CACACAGAGC GAGTGGCGGC TGTACTGTGT GCGCGGAGAG 
GTGCCTGTGG AGGGGAACTT GCTGGAGGTG GCCTGTCACT GCAGCAGCCT CAGGTCTAGG 
ACGTCCATGG TTGTCCTCAA CGTCCACAAA GCCCTCATCT ACCTGTGGCA TGGATGCAAA 
GCCCAGGCCC ACACGAAGGA GGTCGGACGG ACTGCAGCCA ATAAAATCAA GGACCAATGT 
CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC AAAGTGACAA TTCATGAGTG TGATGAAGGC 
TCGGAGCCCC TGGGGTTCTG GGACGCTCTG GGGAGGAGAG ACCGGAAAGC CTACGACTGC 
ATGCTTCAAG ATCCTGGAAA TTTTAACTTC ACACCCCGCC TGTTCATCCT CAGCAGCTCC 
TCTGGTGACT TCTCAGCCAC AGAGTTCATG TATCCCGCCC GAGACCCCTC TGTGGTCAAC 
TCTATGCCCT TCCTGCAGGA AGACCTATAC AGCGCCCCAC AGCCAGCACT CTTCCTTGTT 
GACAATCACC ACGAGGTGTA CCTCTGGCAA GGCTGGTGGC CCATCGAGAA CAAGATCACG 
GGCTCCGCGC GCATCCGCTG GGCCTCGGAC CGGAAGAGCG CCATGGAGAC AGTGCTGCAG 
TACTGCCGAG GGAAAAACCT GAAGAAGCCG CCCCCCAAGT CTTACCTCAT CCACGCTGGC 
CTGGAACCCC TGACCTTCAC CAACATGTTC CCCAGCTGGG AGCACAGAGA AGACATTGCA 
GAGATCACGG AGATGGACAC GGAAGTTTCA AATCAGATCA CCCTAGTGGA GGATGTCTTA 
GCCAAACTGT GTAAGACCAT TTACCCACTG GCCGACCTCC TAGCCAGGCC GCTCCCTGAG 
GGAGTTGACC CTCTGAAGCT GGAGATCTAT CTCACCGACG AGGACTTCGA GTTTGCACTA 
GACATGACCC GAGACGAGTA CAATGCACTG CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA 
GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856031.1
CDS	356..7333
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000263.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000263.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTCCATTC TCGGGTTGAG GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG 
GAGAGAGGGT CCCGGAAACC GAGGCGCTAT TTTTCTCCTG GTGAAAATAG AAAGACTTCC 
GAGAGATTTA GAACTCAACC TATAACCTCG GCAGAACGAA AGGAATCGGA TAGGTCAACT 
TCAAATTCAG AAATGCCAGC TGCCGAGGAT GAAGAAAAGG TCGATGAGCG AGCGAGGCTG 
AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG GAGATGGAGA AGTCGTTTGA CGAGAAGAGC 
GTCCCAAAGC GCCGCTCCCG CAATGCGGCC GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC 
CGGTCCCACA CGCAGCCCGT CACCACCGAG GAGGTGGTCA TTGCAGCCAC TGAACCTACC 
CCCGCTTCGT GCTCAGTGGC CACCCACCCT GTAATGACAA GACATCCTAG CCCCACTGTA 
GCTAAGAGTC CCGTGCAGCC TGCCAGATTG CAGGCGTCTG CTCACCAAAA GGCACTAGCC 
AGAGACCAGA CAAACGAGAG CAAAGATTCT GCTGAGCAGG GAGAACCTGA CTCCTCCACT 
CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT AACAAACTAT CTCAGCCGGT CTCAAAAGCC 
ATTTCAACAC GGAACAGATT AGACATGAGA CAGAGGAGGA TGAATGCTCG TTATCAAACC 
CAGCCGGTCA CGCTTGGAGA AGTGGAACAG GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT 
CCCACCATTA ACACTTCCGT GTCCACCGTG GCATCTACCG TCCCCCCCAT GTATGCAGGA 
AATCTTCGGA CAAAGCCACT TCCAGATGAC AGCTTTGGTG CCACTGAGCA GAAGTTTGCT 
TCTTCGCTAG AAAACTCCGA CTCCCCAGTT AGAAGCATCC TGAAATCCCA AGGCTGGCAG 
CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA ATGTTGAGAG AATTTGAAGA GACAGAACGC 
AAGGGAGGCT TGACAGGTGG AGATGGCGGG GTTACAAAGT ATGGGTCCTT CGAGGAAGCA 
GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC CGGGAGGGAG ACAACCATAA AGAGGCCATC 
TATGCCCTTC CGAGGAAAGG AAGCCTGGAG CTCGCCCATC CTCCCATCGC CCAGCTTGGT 
GATGACCTGA AGGAATTTTC CACCCCAAAG AGCACCATGC AGGCAAGCCC AGACTGGAAG 
GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC TTGGAGAATG TCACAAAGAG GAAGTTTTCG 
CTGAAAGCCG CGGAGTTCGG GGAACCCACT TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA 
CCGGCTGCTC CGACTGCAAC CCCGGTGTCC TGGAAGCCGC AGGATCCCTC CGAACAGCCT 
CAGGAGAAGC GGTATCAGAG CCCGTGTGCG ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG 
GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC AAAACCATGT CCATTAAAGA AAGATTAGCA 
CTGTTGAAGA AGAGCGGTGA GGAAGACTGG AGAAACAGAC TCAACAGAAA GCAGGAATAC 
GGCAAAGCCT CCATCACCAG CAGCCTGCAC ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG 
AAGGAAGAAG GAGGATTTAC AGATGATAAT GACATTTCTA ATCTGCTTTG GGAACCTGTA 
TACGCTTCTA CTTATTCTCC TGCTCTACCT GCTGCCCATA AATACCTGTC TTTTGTATCT 
ATTAATCAGC GGGTCACAGA AAGTCGAGAG AGCCAGATGA CTATTGAAGA GAGAAAGCAT 
CTCATCACCG TGAGAGAGGA CGCCTGGAAG ACCAGGGGCA AAGGAGCAGC CAACGACTCT 
ACCCAGTTTA CTGTGGCCGG CAGGATGGTG AAGCGAGGTC TGGCGTCCCC CACTGCCATC 
ACCCCGGTAG CATCCCCTGT TTCCAGCAAA GCAAGGGGCA CTACACCAGT TTCCAGACCC 
CTGGAAGATA TCGAAGCCAG ACCAGACATG CAGTTAGAGT CGGACCTCAA GCTGGACAGG 
CTGGAAACCT TCCTAAGAAG GCTGAATAAC AAAGTTGGTG GGATGCAAGA AACAGTCCTC 
ACTGTCACTG GGAAATCGGT GAAAGAGGTG ATGAAGCCGG ACGATGATGA AACCTTTGCC 
AAGTTTTACC GCAGCGTGGA CTCCTCTCTA CCGAGAAGCC CCGTAGAGCT GGACGAGGAT 
TTCGATGTCA TTTTTGATCC TTATGCCCCC AGGTTGACAT CCTCGGTGGC TGAGCACAAG 
CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG GCTTCCAAAA ACCCCCTGAA GATGCTGGCC 
GCGAGGGAAG ATCTCCTGCA GGAGTACACT GAGCAGAGAC TGAACGTGGC CTTCGTGGAG 
TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC GCCAACTCCA GCTTCTCAGA AGTCACCCTG 
GCGGGGTTAG CCAGCAAAGA AAACTTCAGC AACGTCAGCC TGCGGAGCGT CAACCTGACG 
GAACAGAATT CCAACAACAG TGCAGTGCCC TACAAGAAGC TGATGCTGCT GCAGGTTAAA 
GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG GAGCCTCGCG CCCCCTCCCT CAACAGCGGG 
GACTGCTTCC TCCTGCTCTC GCCCCATCAC TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC 
GTGATCGAGA AGGCGAAGGC CTCAGAACTT GCGAGTTTAA TTCAGACCAA GAGGGAACTT 
GGTTGTAGAG CAACTTACAT CCAGACTGTT GAAGAAGGAA TTAATACACA CACACATGCA 
GCCAAAGACT TCTGGAAGCT TCTGGGCGGC CAAGCCAGTT ACCAATCCGC CGGAGACCCA 
AAGGAGGATG AGCTCTATGA AACGGCCATA ATAGAGACCA ACTGCATTTA CCGTCTGATG 
GATGACAAAC TCGTTCCTGA TGATGACTAC TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG 
CAATCAAAAG AGGTACTGGT GTTTGATTTT GGGAGTGAAG TTTACGTGTG GCATGGAAAA 
GAAGTCACAT TAGCACAACG GAAAATCGCC TTTCAGCTGG CAAAGCACTT GTGGAATGGA 
ACCTTTGACT ACGAGAATTG TGACATTAAC CCGCTGGATC CTGGGGAATG CAATCCCCTC 
ATTCCCAGGA AAGGACAGGG GCGGCCCGAC TGGGCAATAT TTGGGAGACT TACAGAACAC 
AATGAGACGA TCTTGTTCAA AGAGAAATTC CTTGATTGGA CGGAACTGAA GAGACCTAAC 
GAGAAGAACG CCAGCGAACT CGCGCAGCAC AAGGACGATG CCCGGGCAGA GGTCAAGCCT 
TACGACGTGA CGCGGATGGT GCCCGTGCCC CAGACAACAG CCGGCACCGT GCTGGATGGG 
GTCAACGTGG GCCGAGGCTA CGGGCTGGTG GAAGGGGACG ACCGGAGGCA GTTCGAGATC 
GCCAGCATCT CAGTGGACGT CTGGCACATC CTGGAGTTCG ACTACAGCAG GCTCCCCAAG 
CAGAGCATCG GGCAGTTCCA CGAAGGCGAT GCCTACGTGG TGAAGTGGAA GTTCATCGTG 
AGCACTGCAG TGGGAAGCCG GCAGAAGGGG GAGCACTCGG TCAGGGTGGC TGGCAAAGAG 
AAATGTGTCT ACTTCTTCTG GCAAGGCCGC CAGTCGACCG TGAGTGAGAA GGGCACATCA 
GCTCTGATGA CAGTGGAGCT GGACGAGGAA AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC 
AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA 
CGGGAGGAGG AGGAAGAGAA CACACAGAGC GAGTGGCGGC TGTACTGTGT GCGCGGAGAG 
GTGCCTGTGG AGGGGAACTT GCTGGAGGTG GCCTGTCACT GCAGCAGCCT CAGGTCTAGG 
ACGTCCATGG TTGTCCTCAA CGTCCACAAA GCCCTCATCT ACCTGTGGCA TGGATGCAAA 
GCCCAGGCCC ACACGAAGGA GGTCGGACGG ACTGCAGCCA ATAAAATCAA GGACCAATGT 
CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC AAAGTGACAA TTCATGAGTG TGATGAAGGC 
TCGGAGCCCC TGGGGTTCTG GGACGCTCTG GGGAGGAGAG ACCGGAAAGC CTACGACTGC 
ATGCTTCAAG ATCCTGGAAA TTTTAACTTC ACACCCCGCC TGTTCATCCT CAGCAGCTCC 
TCTGGTGACT TCTCAGCCAC AGAGTTCATG TATCCCGCCC GAGACCCCTC TGTGGTCAAC 
TCTATGCCCT TCCTGCAGGA AGACCTATAC AGCGCCCCAC AGCCAGCACT CTTCCTTGTT 
GACAATCACC ACGAGGTGTA CCTCTGGCAA GGCTGGTGGC CCATCGAGAA CAAGATCACG 
GGCTCCGCGC GCATCCGCTG GGCCTCGGAC CGGAAGAGCG CCATGGAGAC AGTGCTGCAG 
TACTGCCGAG GGAAAAACCT GAAGAAGCCG CCCCCCAAGT CTTACCTCAT CCACGCTGGC 
CTGGAACCCC TGACCTTCAC CAACATGTTC CCCAGCTGGG AGCACAGAGA AGACATTGCA 
GAGATCACGG AGATGGACAC GGAAGTTTCA AATCAGATCA CCCTAGTGGA GGATGTCTTA 
GCCAAACTGT GTAAGACCAT TTACCCACTG GCCGACCTCC TAGCCAGGCC GCTCCCTGAG 
GGAGTTGACC CTCTGAAGCT GGAGATCTAT CTCACCGACG AGGACTTCGA GTTTGCACTA 
GACATGACCC GAGACGAGTA CAATGCACTG CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA 
GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000264.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856032.1 Bos taurus supervillin (SVIL), transcript variant X2, mRNA.

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CloneID	OBa221405
Clone ID Related Accession (Same CDS sequence)	XM_025000264.1
Accession Version	XM_025000264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACAG TATCGGGAAA 
CCCAAACATG AGTGGTTTCT CCAGAAAGAT TCCGAAGGGG ACACACCTTC ACTTATCAAC 
TGGCCATCCA GAGTTAAAGT TAGAGAAAAA TTGGTGCGAG AGGAGAGCGC TCGCAGCAGC 
CCTGAACTTA CCTCCGAGTC CTTAACTCAG AGGAGACACC AGACGGCACC AGGCCATTAC 
CTGGCCTTTC AGTCAGAGAA CTCGGCCTTC GATAGGGTCT CGGGCAAGGT GGCCAGCTCC 
GCAAGACAGC CCATCCGCGG CTACGTCCAG CCAGCAGAGC CTGTCCACAC CATCACGCTG 
GTGACCTCAG ACACCCCAGA AAGCATCTCC GAGGGCAGCT GGGTGGGTCC AGCCCCCCAG 
ACTGTCACAA AGCCTCCCCC CTCAAAGGTT CTAGAAGGTG AAAGAAGAGA CACCCCGGTT 
CTCCATATTT GCGAATCCAA AGCAGAAGAT GTGCTCTTCT CTGATGCTCT GGAGAAAACC 
AGGAAGACCC TTGCAGTTTT GGAGGATCGT GGGTCTGGGA GAAGCCAGGA AGCCCCCTCT 
GGAACTGAGG ACTTGAGTCA GCCCGCTGTT GGCATAGTTA CCGCGGAACC TCAGAAGGAA 
TCAGAAAGCC TGGCCCACCC GCCTATGGCT CAGCAGCAGC CCACTGAGAG GATGGGCAGG 
AGTGAGATGG TCATGTATGT TCAGAGCGAG GCTGTGTCCC AAGGTCACAG GAAGGAGGTG 
CCCACGAGGA AACACAGGGT CCTCACCCGC TCCCTGTCCG ACTACACGGG CCCTCCACAG 
CTCCAGGCTC TGAAGGCCAA GGCCCCGGCT CCCAAGCGAG ATGCCGAGTC ACAGACGTCC 
AAGGCCGAGT TGGAACTGGG CCTGCTGGAC ACGAAGGTCT CGGTCGCCCA GCTCCGCAAT 
GCGTTCCTGG AGTCGGCCAG GGCCAGCAGG AAACCCGAGC TCCATTCTCG GGTTGAGGGG 
TCAAGCGAAG GCCCTGGTGT GGAGCGGGAG AGAGGGTCCC GGAAACCGAG GCGCTATTTT 
TCTCCTGGTG AAAATAGAAA GACTTCCGAG AGATTTAGAA CTCAACCTAT AACCTCGGCA 
GAACGAAAGG AATCGGATAG GTCAACTTCA AATTCAGAAA TGCCAGCTGC CGAGGATGAA 
GAAAAGGTCG ATGAGCGAGC GAGGCTGAGC GTCGCTGCTA AGAGGCTGCT CTTCAGGGAG 
ATGGAGAAGT CGTTTGACGA GAAGAGCGTC CCAAAGCGCC GCTCCCGCAA TGCGGCCGTG 
GAGCAGAGGC TGCGGCGCCT CCAGGACCGG TCCCACACGC AGCCCGTCAC CACCGAGGAG 
GTGGTCATTG CAGCCACTGA ACCTACCCCC GCTTCGTGCT CAGTGGCCAC CCACCCTGTA 
ATGACAAGAC ATCCTAGCCC CACTGTAGCT AAGAGTCCCG TGCAGCCTGC CAGATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG GAAGAAGGAG GATTTACAGA TGATAATGAC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAC GCTTCTACTT ATTCTCCTGC TCTACCTGCT 
GCCCATAAAT ACCTGTCTTT TGTATCTATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAGATGACTA TTGAAGAGAG AAAGCATCTC ATCACCGTGA GAGAGGACGC CTGGAAGACC 
AGGGGCAAAG GAGCAGCCAA CGACTCTACC CAGTTTACTG TGGCCGGCAG GATGGTGAAG 
CGAGGTCTGG CGTCCCCCAC TGCCATCACC CCGGTAGCAT CCCCTGTTTC CAGCAAAGCA 
AGGGGCACTA CACCAGTTTC CAGACCCCTG GAAGATATCG AAGCCAGACC AGACATGCAG 
TTAGAGTCGG ACCTCAAGCT GGACAGGCTG GAAACCTTCC TAAGAAGGCT GAATAACAAA 
GTTGGTGGGA TGCAAGAAAC AGTCCTCACT GTCACTGGGA AATCGGTGAA AGAGGTGATG 
AAGCCGGACG ATGATGAAAC CTTTGCCAAG TTTTACCGCA GCGTGGACTC CTCTCTACCG 
AGAAGCCCCG TAGAGCTGGA CGAGGATTTC GATGTCATTT TTGATCCTTA TGCCCCCAGG 
TTGACATCCT CGGTGGCTGA GCACAAGCGT GCCGTGCGGC CCAAGCGCCG GGTCCAGGCT 
TCCAAAAACC CCCTGAAGAT GCTGGCCGCG AGGGAAGATC TCCTGCAGGA GTACACTGAG 
CAGAGACTGA ACGTGGCCTT CGTGGAGTCA AAGCGGATGA AAGTCGAAAA GTTGTCCGCC 
AACTCCAGCT TCTCAGAAGT CACCCTGGCG GGGTTAGCCA GCAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAATTCCA ACAACAGTGC AGTGCCCTAC 
AAGAAGCTGA TGCTGCTGCA GGTTAAAGGA AGAAGACACG TGCAGACGCG GCTGGTGGAG 
CCTCGCGCCC CCTCCCTCAA CAGCGGGGAC TGCTTCCTCC TGCTCTCGCC CCATCACTGC 
TTCCTGTGGG TCGGAGAGTT CGCCAACGTG ATCGAGAAGG CGAAGGCCTC AGAACTTGCG 
AGTTTAATTC AGACCAAGAG GGAACTTGGT TGTAGAGCAA CTTACATCCA GACTGTTGAA 
GAAGGAATTA ATACACACAC ACATGCAGCC AAAGACTTCT GGAAGCTTCT GGGCGGCCAA 
GCCAGTTACC AATCCGCCGG AGACCCAAAG GAGGATGAGC TCTATGAAAC GGCCATAATA 
GAGACCAACT GCATTTACCG TCTGATGGAT GACAAACTCG TTCCTGATGA TGACTACTGG 
GGGAAGATCC CAAAGTGCTC CCTCCTGCAA TCAAAAGAGG TACTGGTGTT TGATTTTGGG 
AGTGAAGTTT ACGTGTGGCA TGGAAAAGAA GTCACATTAG CACAACGGAA AATCGCCTTT 
CAGCTGGCAA AGCACTTGTG GAATGGAACC TTTGACTACG AGAATTGTGA CATTAACCCG 
CTGGATCCTG GGGAATGCAA TCCCCTCATT CCCAGGAAAG GACAGGGGCG GCCCGACTGG 
GCAATATTTG GGAGACTTAC AGAACACAAT GAGACGATCT TGTTCAAAGA GAAATTCCTT 
GATTGGACGG AACTGAAGAG ACCTAACGAG AAGAACGCCA GCGAACTCGC GCAGCACAAG 
GACGATGCCC GGGCAGAGGT CAAGCCTTAC GACGTGACGC GGATGGTGCC CGTGCCCCAG 
ACAACAGCCG GCACCGTGCT GGATGGGGTC AACGTGGGCC GAGGCTACGG GCTGGTGGAA 
GGGGACGACC GGAGGCAGTT CGAGATCGCC AGCATCTCAG TGGACGTCTG GCACATCCTG 
GAGTTCGACT ACAGCAGGCT CCCCAAGCAG AGCATCGGGC AGTTCCACGA AGGCGATGCC 
TACGTGGTGA AGTGGAAGTT CATCGTGAGC ACTGCAGTGG GAAGCCGGCA GAAGGGGGAG 
CACTCGGTCA GGGTGGCTGG CAAAGAGAAA TGTGTCTACT TCTTCTGGCA AGGCCGCCAG 
TCGACCGTGA GTGAGAAGGG CACATCAGCT CTGATGACAG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTCCT GCAGGGCAAG GAGCCCCCCT GTTTCCTGCA GTGCTTCCAG 
GGGGGGATGG TGGTCCACTC CGGGAGACGG GAGGAGGAGG AAGAGAACAC ACAGAGCGAG 
TGGCGGCTGT ACTGTGTGCG CGGAGAGGTG CCTGTGGAGG GGAACTTGCT GGAGGTGGCC 
TGTCACTGCA GCAGCCTCAG GTCTAGGACG TCCATGGTTG TCCTCAACGT CCACAAAGCC 
CTCATCTACC TGTGGCATGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGACGGACT 
GCAGCCAATA AAATCAAGGA CCAATGTCCC CTGGAGGCCG GGCTGCACAG TAGCAGCAAA 
GTGACAATTC ATGAGTGTGA TGAAGGCTCG GAGCCCCTGG GGTTCTGGGA CGCTCTGGGG 
AGGAGAGACC GGAAAGCCTA CGACTGCATG CTTCAAGATC CTGGAAATTT TAACTTCACA 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGTGACTTCT CAGCCACAGA GTTCATGTAT 
CCCGCCCGAG ACCCCTCTGT GGTCAACTCT ATGCCCTTCC TGCAGGAAGA CCTATACAGC 
GCCCCACAGC CAGCACTCTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACGGGC TCCGCGCGCA TCCGCTGGGC CTCGGACCGG 
AAGAGCGCCA TGGAGACAGT GCTGCAGTAC TGCCGAGGGA AAAACCTGAA GAAGCCGCCC 
CCCAAGTCTT ACCTCATCCA CGCTGGCCTG GAACCCCTGA CCTTCACCAA CATGTTCCCC 
AGCTGGGAGC ACAGAGAAGA CATTGCAGAG ATCACGGAGA TGGACACGGA AGTTTCAAAT 
CAGATCACCC TAGTGGAGGA TGTCTTAGCC AAACTGTGTA AGACCATTTA CCCACTGGCC 
GACCTCCTAG CCAGGCCGCT CCCTGAGGGA GTTGACCCTC TGAAGCTGGA GATCTATCTC 
ACCGACGAGG ACTTCGAGTT TGCACTAGAC ATGACCCGAG ACGAGTACAA TGCACTGCCC 
GCTTGGAAGC AGGTGAACCT GAAGAAAGCG AAAGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856032.1
CDS	356..7300
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000264.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000264.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACAG TATCGGGAAA 
CCCAAACATG AGTGGTTTCT CCAGAAAGAT TCCGAAGGGG ACACACCTTC ACTTATCAAC 
TGGCCATCCA GAGTTAAAGT TAGAGAAAAA TTGGTGCGAG AGGAGAGCGC TCGCAGCAGC 
CCTGAACTTA CCTCCGAGTC CTTAACTCAG AGGAGACACC AGACGGCACC AGGCCATTAC 
CTGGCCTTTC AGTCAGAGAA CTCGGCCTTC GATAGGGTCT CGGGCAAGGT GGCCAGCTCC 
GCAAGACAGC CCATCCGCGG CTACGTCCAG CCAGCAGAGC CTGTCCACAC CATCACGCTG 
GTGACCTCAG ACACCCCAGA AAGCATCTCC GAGGGCAGCT GGGTGGGTCC AGCCCCCCAG 
ACTGTCACAA AGCCTCCCCC CTCAAAGGTT CTAGAAGGTG AAAGAAGAGA CACCCCGGTT 
CTCCATATTT GCGAATCCAA AGCAGAAGAT GTGCTCTTCT CTGATGCTCT GGAGAAAACC 
AGGAAGACCC TTGCAGTTTT GGAGGATCGT GGGTCTGGGA GAAGCCAGGA AGCCCCCTCT 
GGAACTGAGG ACTTGAGTCA GCCCGCTGTT GGCATAGTTA CCGCGGAACC TCAGAAGGAA 
TCAGAAAGCC TGGCCCACCC GCCTATGGCT CAGCAGCAGC CCACTGAGAG GATGGGCAGG 
AGTGAGATGG TCATGTATGT TCAGAGCGAG GCTGTGTCCC AAGGTCACAG GAAGGAGGTG 
CCCACGAGGA AACACAGGGT CCTCACCCGC TCCCTGTCCG ACTACACGGG CCCTCCACAG 
CTCCAGGCTC TGAAGGCCAA GGCCCCGGCT CCCAAGCGAG ATGCCGAGTC ACAGACGTCC 
AAGGCCGAGT TGGAACTGGG CCTGCTGGAC ACGAAGGTCT CGGTCGCCCA GCTCCGCAAT 
GCGTTCCTGG AGTCGGCCAG GGCCAGCAGG AAACCCGAGC TCCATTCTCG GGTTGAGGGG 
TCAAGCGAAG GCCCTGGTGT GGAGCGGGAG AGAGGGTCCC GGAAACCGAG GCGCTATTTT 
TCTCCTGGTG AAAATAGAAA GACTTCCGAG AGATTTAGAA CTCAACCTAT AACCTCGGCA 
GAACGAAAGG AATCGGATAG GTCAACTTCA AATTCAGAAA TGCCAGCTGC CGAGGATGAA 
GAAAAGGTCG ATGAGCGAGC GAGGCTGAGC GTCGCTGCTA AGAGGCTGCT CTTCAGGGAG 
ATGGAGAAGT CGTTTGACGA GAAGAGCGTC CCAAAGCGCC GCTCCCGCAA TGCGGCCGTG 
GAGCAGAGGC TGCGGCGCCT CCAGGACCGG TCCCACACGC AGCCCGTCAC CACCGAGGAG 
GTGGTCATTG CAGCCACTGA ACCTACCCCC GCTTCGTGCT CAGTGGCCAC CCACCCTGTA 
ATGACAAGAC ATCCTAGCCC CACTGTAGCT AAGAGTCCCG TGCAGCCTGC CAGATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG GAAGAAGGAG GATTTACAGA TGATAATGAC 
ATTTCTAATC TGCTTTGGGA ACCTGTATAC GCTTCTACTT ATTCTCCTGC TCTACCTGCT 
GCCCATAAAT ACCTGTCTTT TGTATCTATT AATCAGCGGG TCACAGAAAG TCGAGAGAGC 
CAGATGACTA TTGAAGAGAG AAAGCATCTC ATCACCGTGA GAGAGGACGC CTGGAAGACC 
AGGGGCAAAG GAGCAGCCAA CGACTCTACC CAGTTTACTG TGGCCGGCAG GATGGTGAAG 
CGAGGTCTGG CGTCCCCCAC TGCCATCACC CCGGTAGCAT CCCCTGTTTC CAGCAAAGCA 
AGGGGCACTA CACCAGTTTC CAGACCCCTG GAAGATATCG AAGCCAGACC AGACATGCAG 
TTAGAGTCGG ACCTCAAGCT GGACAGGCTG GAAACCTTCC TAAGAAGGCT GAATAACAAA 
GTTGGTGGGA TGCAAGAAAC AGTCCTCACT GTCACTGGGA AATCGGTGAA AGAGGTGATG 
AAGCCGGACG ATGATGAAAC CTTTGCCAAG TTTTACCGCA GCGTGGACTC CTCTCTACCG 
AGAAGCCCCG TAGAGCTGGA CGAGGATTTC GATGTCATTT TTGATCCTTA TGCCCCCAGG 
TTGACATCCT CGGTGGCTGA GCACAAGCGT GCCGTGCGGC CCAAGCGCCG GGTCCAGGCT 
TCCAAAAACC CCCTGAAGAT GCTGGCCGCG AGGGAAGATC TCCTGCAGGA GTACACTGAG 
CAGAGACTGA ACGTGGCCTT CGTGGAGTCA AAGCGGATGA AAGTCGAAAA GTTGTCCGCC 
AACTCCAGCT TCTCAGAAGT CACCCTGGCG GGGTTAGCCA GCAAAGAAAA CTTCAGCAAC 
GTCAGCCTGC GGAGCGTCAA CCTGACGGAA CAGAATTCCA ACAACAGTGC AGTGCCCTAC 
AAGAAGCTGA TGCTGCTGCA GGTTAAAGGA AGAAGACACG TGCAGACGCG GCTGGTGGAG 
CCTCGCGCCC CCTCCCTCAA CAGCGGGGAC TGCTTCCTCC TGCTCTCGCC CCATCACTGC 
TTCCTGTGGG TCGGAGAGTT CGCCAACGTG ATCGAGAAGG CGAAGGCCTC AGAACTTGCG 
AGTTTAATTC AGACCAAGAG GGAACTTGGT TGTAGAGCAA CTTACATCCA GACTGTTGAA 
GAAGGAATTA ATACACACAC ACATGCAGCC AAAGACTTCT GGAAGCTTCT GGGCGGCCAA 
GCCAGTTACC AATCCGCCGG AGACCCAAAG GAGGATGAGC TCTATGAAAC GGCCATAATA 
GAGACCAACT GCATTTACCG TCTGATGGAT GACAAACTCG TTCCTGATGA TGACTACTGG 
GGGAAGATCC CAAAGTGCTC CCTCCTGCAA TCAAAAGAGG TACTGGTGTT TGATTTTGGG 
AGTGAAGTTT ACGTGTGGCA TGGAAAAGAA GTCACATTAG CACAACGGAA AATCGCCTTT 
CAGCTGGCAA AGCACTTGTG GAATGGAACC TTTGACTACG AGAATTGTGA CATTAACCCG 
CTGGATCCTG GGGAATGCAA TCCCCTCATT CCCAGGAAAG GACAGGGGCG GCCCGACTGG 
GCAATATTTG GGAGACTTAC AGAACACAAT GAGACGATCT TGTTCAAAGA GAAATTCCTT 
GATTGGACGG AACTGAAGAG ACCTAACGAG AAGAACGCCA GCGAACTCGC GCAGCACAAG 
GACGATGCCC GGGCAGAGGT CAAGCCTTAC GACGTGACGC GGATGGTGCC CGTGCCCCAG 
ACAACAGCCG GCACCGTGCT GGATGGGGTC AACGTGGGCC GAGGCTACGG GCTGGTGGAA 
GGGGACGACC GGAGGCAGTT CGAGATCGCC AGCATCTCAG TGGACGTCTG GCACATCCTG 
GAGTTCGACT ACAGCAGGCT CCCCAAGCAG AGCATCGGGC AGTTCCACGA AGGCGATGCC 
TACGTGGTGA AGTGGAAGTT CATCGTGAGC ACTGCAGTGG GAAGCCGGCA GAAGGGGGAG 
CACTCGGTCA GGGTGGCTGG CAAAGAGAAA TGTGTCTACT TCTTCTGGCA AGGCCGCCAG 
TCGACCGTGA GTGAGAAGGG CACATCAGCT CTGATGACAG TGGAGCTGGA CGAGGAAAGG 
GGGGCCCAGG TCCAGGTCCT GCAGGGCAAG GAGCCCCCCT GTTTCCTGCA GTGCTTCCAG 
GGGGGGATGG TGGTCCACTC CGGGAGACGG GAGGAGGAGG AAGAGAACAC ACAGAGCGAG 
TGGCGGCTGT ACTGTGTGCG CGGAGAGGTG CCTGTGGAGG GGAACTTGCT GGAGGTGGCC 
TGTCACTGCA GCAGCCTCAG GTCTAGGACG TCCATGGTTG TCCTCAACGT CCACAAAGCC 
CTCATCTACC TGTGGCATGG ATGCAAAGCC CAGGCCCACA CGAAGGAGGT CGGACGGACT 
GCAGCCAATA AAATCAAGGA CCAATGTCCC CTGGAGGCCG GGCTGCACAG TAGCAGCAAA 
GTGACAATTC ATGAGTGTGA TGAAGGCTCG GAGCCCCTGG GGTTCTGGGA CGCTCTGGGG 
AGGAGAGACC GGAAAGCCTA CGACTGCATG CTTCAAGATC CTGGAAATTT TAACTTCACA 
CCCCGCCTGT TCATCCTCAG CAGCTCCTCT GGTGACTTCT CAGCCACAGA GTTCATGTAT 
CCCGCCCGAG ACCCCTCTGT GGTCAACTCT ATGCCCTTCC TGCAGGAAGA CCTATACAGC 
GCCCCACAGC CAGCACTCTT CCTTGTTGAC AATCACCACG AGGTGTACCT CTGGCAAGGC 
TGGTGGCCCA TCGAGAACAA GATCACGGGC TCCGCGCGCA TCCGCTGGGC CTCGGACCGG 
AAGAGCGCCA TGGAGACAGT GCTGCAGTAC TGCCGAGGGA AAAACCTGAA GAAGCCGCCC 
CCCAAGTCTT ACCTCATCCA CGCTGGCCTG GAACCCCTGA CCTTCACCAA CATGTTCCCC 
AGCTGGGAGC ACAGAGAAGA CATTGCAGAG ATCACGGAGA TGGACACGGA AGTTTCAAAT 
CAGATCACCC TAGTGGAGGA TGTCTTAGCC AAACTGTGTA AGACCATTTA CCCACTGGCC 
GACCTCCTAG CCAGGCCGCT CCCTGAGGGA GTTGACCCTC TGAAGCTGGA GATCTATCTC 
ACCGACGAGG ACTTCGAGTT TGCACTAGAC ATGACCCGAG ACGAGTACAA TGCACTGCCC 
GCTTGGAAGC AGGTGAACCT GAAGAAAGCG AAAGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000265.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856033.1 Bos taurus supervillin (SVIL), transcript variant X3, mRNA.

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CloneID	OBa221406
Clone ID Related Accession (Same CDS sequence)	XM_025000265.1
Accession Version	XM_025000265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGAGT TAAAGTTAGA 
GAAAAATTGG TGCGAGAGGA GAGCGCTCGC AGCAGCCCTG AACTTACCTC CGAGTCCTTA 
ACTCAGAGGA GACACCAGAC GGCACCAGGC CATTACCTGG CCTTTCAGTC AGAGAACTCG 
GCCTTCGATA GGGTCTCGGG CAAGGTGGCC AGCTCCGCAA GACAGCCCAT CCGCGGCTAC 
GTCCAGCCAG CAGAGCCTGT CCACACCATC ACGCTGGTGA CCTCAGACAC CCCAGAAAGC 
ATCTCCGAGG GCAGCTGGGT GGGTCCAGCC CCCCAGACTG TCACAAAGCC TCCCCCCTCA 
AAGGTTCTAG AAGGTGAAAG AAGAGACACC CCGGTTCTCC ATATTTGCGA ATCCAAAGCA 
GAAGATGTGC TCTTCTCTGA TGCTCTGGAG AAAACCAGGA AGACCCTTGC AGTTTTGGAG 
GATCGTGGGT CTGGGAGAAG CCAGGAAGCC CCCTCTGGAA CTGAGGACTT GAGTCAGCCC 
GCTGTTGGCA TAGTTACCGC GGAACCTCAG AAGGAATCAG AAAGCCTGGC CCACCCGCCT 
ATGGCTCAGC AGCAGCCCAC TGAGAGGATG GGCAGGAGTG AGATGGTCAT GTATGTTCAG 
AGCGAGGCTG TGTCCCAAGG TCACAGGAAG GAGGTGCCCA CGAGGAAACA CAGGGTCCTC 
ACCCGCTCCC TGTCCGACTA CACGGGCCCT CCACAGCTCC AGGCTCTGAA GGCCAAGGCC 
CCGGCTCCCA AGCGAGATGC CGAGTCACAG ACGTCCAAGG CCGAGTTGGA ACTGGGCCTG 
CTGGACACGA AGGTCTCGGT CGCCCAGCTC CGCAATGCGT TCCTGGAGTC GGCCAGGGCC 
AGCAGGAAAC CCGAGCTCCA TTCTCGGGTT GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG 
CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC TATTTTTCTC CTGGTGAAAA TAGAAAGACT 
TCCGAGAGAT TTAGAACTCA ACCTATAACC TCGGCAGAAC GAAAGGAATC GGATAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACTGAACCT 
ACCCCCGCTT CGTGCTCAGT GGCCACCCAC CCTGTAATGA CAAGACATCC TAGCCCCACT 
GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA TTGCAGGCGT CTGCTCACCA AAAGGCACTA 
GCCAGAGACC AGACAAACGA GAGCAAAGAT TCTGCTGAGC AGGGAGAACC TGACTCCTCC 
ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG TTTAACAAAC TATCTCAGCC GGTCTCAAAA 
GCCATTTCAA CACGGAACAG ATTAGACATG AGACAGAGGA GGATGAATGC TCGTTATCAA 
ACCCAGCCGG TCACGCTTGG AGAAGTGGAA CAGGTGCAGA GTGGCAAGCT CATGGCTTTC 
TCTCCCACCA TTAACACTTC CGTGTCCACC GTGGCATCTA CCGTCCCCCC CATGTATGCA 
GGAAATCTTC GGACAAAGCC ACTTCCAGAT GACAGCTTTG GTGCCACTGA GCAGAAGTTT 
GCTTCTTCGC TAGAAAACTC CGACTCCCCA GTTAGAAGCA TCCTGAAATC CCAAGGCTGG 
CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA GCAATGTTGA GAGAATTTGA AGAGACAGAA 
CGCAAGGGAG GCTTGACAGG TGGAGATGGC GGGGTTACAA AGTATGGGTC CTTCGAGGAA 
GCAGAGCTGT CCTACCCCGT CCTCAGCAGA GTCCGGGAGG GAGACAACCA TAAAGAGGCC 
ATCTATGCCC TTCCGAGGAA AGGAAGCCTG GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT 
GGTGATGACC TGAAGGAATT TTCCACCCCA AAGAGCACCA TGCAGGCAAG CCCAGACTGG 
AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG GACTTGGAGA ATGTCACAAA GAGGAAGTTT 
TCGCTGAAAG CCGCGGAGTT CGGGGAACCC ACTTCTGAGC AGACCGGCGC GGCTGCTGGG 
AAACCGGCTG CTCCGACTGC AACCCCGGTG TCCTGGAAGC CGCAGGATCC CTCCGAACAG 
CCTCAGGAGA AGCGGTATCA GAGCCCGTGT GCGATGTTTG CTGCTGGAGA GATCAAAGCC 
CCGGCGGTGG AGGGCAGCCT CGACTCCCCC AGCAAAACCA TGTCCATTAA AGAAAGATTA 
GCACTGTTGA AGAAGAGCGG TGAGGAAGAC TGGAGAAACA GACTCAACAG AAAGCAGGAA 
TACGGCAAAG CCTCCATCAC CAGCAGCCTG CACATCCAAG AGACGGAGCA GTCACTCAAG 
AAGAAGGAAG AAGGAGGATT TACAGATGAT AATGACATTT CTAATCTGCT TTGGGAACCT 
GTATACGCTT CTACTTATTC TCCTGCTCTA CCTGCTGCCC ATAAATACCT GTCTTTTGTA 
TCTATTAATC AGCGGGTCAC AGAAAGTCGA GAGAGCCAGA TGACTATTGA AGAGAGAAAG 
CATCTCATCA CCGTGAGAGA GGACGCCTGG AAGACCAGGG GCAAAGGAGC AGCCAACGAC 
TCTACCCAGT TTACTGTGGC CGGCAGGATG GTGAAGCGAG GTCTGGCGTC CCCCACTGCC 
ATCACCCCGG TAGCATCCCC TGTTTCCAGC AAAGCAAGGG GCACTACACC AGTTTCCAGA 
CCCCTGGAAG ATATCGAAGC CAGACCAGAC ATGCAGTTAG AGTCGGACCT CAAGCTGGAC 
AGGCTGGAAA CCTTCCTAAG AAGGCTGAAT AACAAAGTTG GTGGGATGCA AGAAACAGTC 
CTCACTGTCA CTGGGAAATC GGTGAAAGAG GTGATGAAGC CGGACGATGA TGAAACCTTT 
GCCAAGTTTT ACCGCAGCGT GGACTCCTCT CTACCGAGAA GCCCCGTAGA GCTGGACGAG 
GATTTCGATG TCATTTTTGA TCCTTATGCC CCCAGGTTGA CATCCTCGGT GGCTGAGCAC 
AAGCGTGCCG TGCGGCCCAA GCGCCGGGTC CAGGCTTCCA AAAACCCCCT GAAGATGCTG 
GCCGCGAGGG AAGATCTCCT GCAGGAGTAC ACTGAGCAGA GACTGAACGT GGCCTTCGTG 
GAGTCAAAGC GGATGAAAGT CGAAAAGTTG TCCGCCAACT CCAGCTTCTC AGAAGTCACC 
CTGGCGGGGT TAGCCAGCAA AGAAAACTTC AGCAACGTCA GCCTGCGGAG CGTCAACCTG 
ACGGAACAGA ATTCCAACAA CAGTGCAGTG CCCTACAAGA AGCTGATGCT GCTGCAGGTT 
AAAGGAAGAA GACACGTGCA GACGCGGCTG GTGGAGCCTC GCGCCCCCTC CCTCAACAGC 
GGGGACTGCT TCCTCCTGCT CTCGCCCCAT CACTGCTTCC TGTGGGTCGG AGAGTTCGCC 
AACGTGATCG AGAAGGCGAA GGCCTCAGAA CTTGCGAGTT TAATTCAGAC CAAGAGGGAA 
CTTGGTTGTA GAGCAACTTA CATCCAGACT GTTGAAGAAG GAATTAATAC ACACACACAT 
GCAGCCAAAG ACTTCTGGAA GCTTCTGGGC GGCCAAGCCA GTTACCAATC CGCCGGAGAC 
CCAAAGGAGG ATGAGCTCTA TGAAACGGCC ATAATAGAGA CCAACTGCAT TTACCGTCTG 
ATGGATGACA AACTCGTTCC TGATGATGAC TACTGGGGGA AGATCCCAAA GTGCTCCCTC 
CTGCAATCAA AAGAGGTACT GGTGTTTGAT TTTGGGAGTG AAGTTTACGT GTGGCATGGA 
AAAGAAGTCA CATTAGCACA ACGGAAAATC GCCTTTCAGC TGGCAAAGCA CTTGTGGAAT 
GGAACCTTTG ACTACGAGAA TTGTGACATT AACCCGCTGG ATCCTGGGGA ATGCAATCCC 
CTCATTCCCA GGAAAGGACA GGGGCGGCCC GACTGGGCAA TATTTGGGAG ACTTACAGAA 
CACAATGAGA CGATCTTGTT CAAAGAGAAA TTCCTTGATT GGACGGAACT GAAGAGACCT 
AACGAGAAGA ACGCCAGCGA ACTCGCGCAG CACAAGGACG ATGCCCGGGC AGAGGTCAAG 
CCTTACGACG TGACGCGGAT GGTGCCCGTG CCCCAGACAA CAGCCGGCAC CGTGCTGGAT 
GGGGTCAACG TGGGCCGAGG CTACGGGCTG GTGGAAGGGG ACGACCGGAG GCAGTTCGAG 
ATCGCCAGCA TCTCAGTGGA CGTCTGGCAC ATCCTGGAGT TCGACTACAG CAGGCTCCCC 
AAGCAGAGCA TCGGGCAGTT CCACGAAGGC GATGCCTACG TGGTGAAGTG GAAGTTCATC 
GTGAGCACTG CAGTGGGAAG CCGGCAGAAG GGGGAGCACT CGGTCAGGGT GGCTGGCAAA 
GAGAAATGTG TCTACTTCTT CTGGCAAGGC CGCCAGTCGA CCGTGAGTGA GAAGGGCACA 
TCAGCTCTGA TGACAGTGGA GCTGGACGAG GAAAGGGGGG CCCAGGTCCA GGTCCTGCAG 
GGCAAGGAGC CCCCCTGTTT CCTGCAGTGC TTCCAGGGGG GGATGGTGGT CCACTCCGGG 
AGACGGGAGG AGGAGGAAGA GAACACACAG AGCGAGTGGC GGCTGTACTG TGTGCGCGGA 
GAGGTGCCTG TGGAGGGGAA CTTGCTGGAG GTGGCCTGTC ACTGCAGCAG CCTCAGGTCT 
AGGACGTCCA TGGTTGTCCT CAACGTCCAC AAAGCCCTCA TCTACCTGTG GCATGGATGC 
AAAGCCCAGG CCCACACGAA GGAGGTCGGA CGGACTGCAG CCAATAAAAT CAAGGACCAA 
TGTCCCCTGG AGGCCGGGCT GCACAGTAGC AGCAAAGTGA CAATTCATGA GTGTGATGAA 
GGCTCGGAGC CCCTGGGGTT CTGGGACGCT CTGGGGAGGA GAGACCGGAA AGCCTACGAC 
TGCATGCTTC AAGATCCTGG AAATTTTAAC TTCACACCCC GCCTGTTCAT CCTCAGCAGC 
TCCTCTGGTG ACTTCTCAGC CACAGAGTTC ATGTATCCCG CCCGAGACCC CTCTGTGGTC 
AACTCTATGC CCTTCCTGCA GGAAGACCTA TACAGCGCCC CACAGCCAGC ACTCTTCCTT 
GTTGACAATC ACCACGAGGT GTACCTCTGG CAAGGCTGGT GGCCCATCGA GAACAAGATC 
ACGGGCTCCG CGCGCATCCG CTGGGCCTCG GACCGGAAGA GCGCCATGGA GACAGTGCTG 
CAGTACTGCC GAGGGAAAAA CCTGAAGAAG CCGCCCCCCA AGTCTTACCT CATCCACGCT 
GGCCTGGAAC CCCTGACCTT CACCAACATG TTCCCCAGCT GGGAGCACAG AGAAGACATT 
GCAGAGATCA CGGAGATGGA CACGGAAGTT TCAAATCAGA TCACCCTAGT GGAGGATGTC 
TTAGCCAAAC TGTGTAAGAC CATTTACCCA CTGGCCGACC TCCTAGCCAG GCCGCTCCCT 
GAGGGAGTTG ACCCTCTGAA GCTGGAGATC TATCTCACCG ACGAGGACTT CGAGTTTGCA 
CTAGACATGA CCCGAGACGA GTACAATGCA CTGCCCGCTT GGAAGCAGGT GAACCTGAAG 
AAAGCGAAAG GTCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856033.1
CDS	356..7276
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000265.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000265.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGAGT TAAAGTTAGA 
GAAAAATTGG TGCGAGAGGA GAGCGCTCGC AGCAGCCCTG AACTTACCTC CGAGTCCTTA 
ACTCAGAGGA GACACCAGAC GGCACCAGGC CATTACCTGG CCTTTCAGTC AGAGAACTCG 
GCCTTCGATA GGGTCTCGGG CAAGGTGGCC AGCTCCGCAA GACAGCCCAT CCGCGGCTAC 
GTCCAGCCAG CAGAGCCTGT CCACACCATC ACGCTGGTGA CCTCAGACAC CCCAGAAAGC 
ATCTCCGAGG GCAGCTGGGT GGGTCCAGCC CCCCAGACTG TCACAAAGCC TCCCCCCTCA 
AAGGTTCTAG AAGGTGAAAG AAGAGACACC CCGGTTCTCC ATATTTGCGA ATCCAAAGCA 
GAAGATGTGC TCTTCTCTGA TGCTCTGGAG AAAACCAGGA AGACCCTTGC AGTTTTGGAG 
GATCGTGGGT CTGGGAGAAG CCAGGAAGCC CCCTCTGGAA CTGAGGACTT GAGTCAGCCC 
GCTGTTGGCA TAGTTACCGC GGAACCTCAG AAGGAATCAG AAAGCCTGGC CCACCCGCCT 
ATGGCTCAGC AGCAGCCCAC TGAGAGGATG GGCAGGAGTG AGATGGTCAT GTATGTTCAG 
AGCGAGGCTG TGTCCCAAGG TCACAGGAAG GAGGTGCCCA CGAGGAAACA CAGGGTCCTC 
ACCCGCTCCC TGTCCGACTA CACGGGCCCT CCACAGCTCC AGGCTCTGAA GGCCAAGGCC 
CCGGCTCCCA AGCGAGATGC CGAGTCACAG ACGTCCAAGG CCGAGTTGGA ACTGGGCCTG 
CTGGACACGA AGGTCTCGGT CGCCCAGCTC CGCAATGCGT TCCTGGAGTC GGCCAGGGCC 
AGCAGGAAAC CCGAGCTCCA TTCTCGGGTT GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG 
CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC TATTTTTCTC CTGGTGAAAA TAGAAAGACT 
TCCGAGAGAT TTAGAACTCA ACCTATAACC TCGGCAGAAC GAAAGGAATC GGATAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACTGAACCT 
ACCCCCGCTT CGTGCTCAGT GGCCACCCAC CCTGTAATGA CAAGACATCC TAGCCCCACT 
GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA TTGCAGGCGT CTGCTCACCA AAAGGCACTA 
GCCAGAGACC AGACAAACGA GAGCAAAGAT TCTGCTGAGC AGGGAGAACC TGACTCCTCC 
ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG TTTAACAAAC TATCTCAGCC GGTCTCAAAA 
GCCATTTCAA CACGGAACAG ATTAGACATG AGACAGAGGA GGATGAATGC TCGTTATCAA 
ACCCAGCCGG TCACGCTTGG AGAAGTGGAA CAGGTGCAGA GTGGCAAGCT CATGGCTTTC 
TCTCCCACCA TTAACACTTC CGTGTCCACC GTGGCATCTA CCGTCCCCCC CATGTATGCA 
GGAAATCTTC GGACAAAGCC ACTTCCAGAT GACAGCTTTG GTGCCACTGA GCAGAAGTTT 
GCTTCTTCGC TAGAAAACTC CGACTCCCCA GTTAGAAGCA TCCTGAAATC CCAAGGCTGG 
CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA GCAATGTTGA GAGAATTTGA AGAGACAGAA 
CGCAAGGGAG GCTTGACAGG TGGAGATGGC GGGGTTACAA AGTATGGGTC CTTCGAGGAA 
GCAGAGCTGT CCTACCCCGT CCTCAGCAGA GTCCGGGAGG GAGACAACCA TAAAGAGGCC 
ATCTATGCCC TTCCGAGGAA AGGAAGCCTG GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT 
GGTGATGACC TGAAGGAATT TTCCACCCCA AAGAGCACCA TGCAGGCAAG CCCAGACTGG 
AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG GACTTGGAGA ATGTCACAAA GAGGAAGTTT 
TCGCTGAAAG CCGCGGAGTT CGGGGAACCC ACTTCTGAGC AGACCGGCGC GGCTGCTGGG 
AAACCGGCTG CTCCGACTGC AACCCCGGTG TCCTGGAAGC CGCAGGATCC CTCCGAACAG 
CCTCAGGAGA AGCGGTATCA GAGCCCGTGT GCGATGTTTG CTGCTGGAGA GATCAAAGCC 
CCGGCGGTGG AGGGCAGCCT CGACTCCCCC AGCAAAACCA TGTCCATTAA AGAAAGATTA 
GCACTGTTGA AGAAGAGCGG TGAGGAAGAC TGGAGAAACA GACTCAACAG AAAGCAGGAA 
TACGGCAAAG CCTCCATCAC CAGCAGCCTG CACATCCAAG AGACGGAGCA GTCACTCAAG 
AAGAAGGAAG AAGGAGGATT TACAGATGAT AATGACATTT CTAATCTGCT TTGGGAACCT 
GTATACGCTT CTACTTATTC TCCTGCTCTA CCTGCTGCCC ATAAATACCT GTCTTTTGTA 
TCTATTAATC AGCGGGTCAC AGAAAGTCGA GAGAGCCAGA TGACTATTGA AGAGAGAAAG 
CATCTCATCA CCGTGAGAGA GGACGCCTGG AAGACCAGGG GCAAAGGAGC AGCCAACGAC 
TCTACCCAGT TTACTGTGGC CGGCAGGATG GTGAAGCGAG GTCTGGCGTC CCCCACTGCC 
ATCACCCCGG TAGCATCCCC TGTTTCCAGC AAAGCAAGGG GCACTACACC AGTTTCCAGA 
CCCCTGGAAG ATATCGAAGC CAGACCAGAC ATGCAGTTAG AGTCGGACCT CAAGCTGGAC 
AGGCTGGAAA CCTTCCTAAG AAGGCTGAAT AACAAAGTTG GTGGGATGCA AGAAACAGTC 
CTCACTGTCA CTGGGAAATC GGTGAAAGAG GTGATGAAGC CGGACGATGA TGAAACCTTT 
GCCAAGTTTT ACCGCAGCGT GGACTCCTCT CTACCGAGAA GCCCCGTAGA GCTGGACGAG 
GATTTCGATG TCATTTTTGA TCCTTATGCC CCCAGGTTGA CATCCTCGGT GGCTGAGCAC 
AAGCGTGCCG TGCGGCCCAA GCGCCGGGTC CAGGCTTCCA AAAACCCCCT GAAGATGCTG 
GCCGCGAGGG AAGATCTCCT GCAGGAGTAC ACTGAGCAGA GACTGAACGT GGCCTTCGTG 
GAGTCAAAGC GGATGAAAGT CGAAAAGTTG TCCGCCAACT CCAGCTTCTC AGAAGTCACC 
CTGGCGGGGT TAGCCAGCAA AGAAAACTTC AGCAACGTCA GCCTGCGGAG CGTCAACCTG 
ACGGAACAGA ATTCCAACAA CAGTGCAGTG CCCTACAAGA AGCTGATGCT GCTGCAGGTT 
AAAGGAAGAA GACACGTGCA GACGCGGCTG GTGGAGCCTC GCGCCCCCTC CCTCAACAGC 
GGGGACTGCT TCCTCCTGCT CTCGCCCCAT CACTGCTTCC TGTGGGTCGG AGAGTTCGCC 
AACGTGATCG AGAAGGCGAA GGCCTCAGAA CTTGCGAGTT TAATTCAGAC CAAGAGGGAA 
CTTGGTTGTA GAGCAACTTA CATCCAGACT GTTGAAGAAG GAATTAATAC ACACACACAT 
GCAGCCAAAG ACTTCTGGAA GCTTCTGGGC GGCCAAGCCA GTTACCAATC CGCCGGAGAC 
CCAAAGGAGG ATGAGCTCTA TGAAACGGCC ATAATAGAGA CCAACTGCAT TTACCGTCTG 
ATGGATGACA AACTCGTTCC TGATGATGAC TACTGGGGGA AGATCCCAAA GTGCTCCCTC 
CTGCAATCAA AAGAGGTACT GGTGTTTGAT TTTGGGAGTG AAGTTTACGT GTGGCATGGA 
AAAGAAGTCA CATTAGCACA ACGGAAAATC GCCTTTCAGC TGGCAAAGCA CTTGTGGAAT 
GGAACCTTTG ACTACGAGAA TTGTGACATT AACCCGCTGG ATCCTGGGGA ATGCAATCCC 
CTCATTCCCA GGAAAGGACA GGGGCGGCCC GACTGGGCAA TATTTGGGAG ACTTACAGAA 
CACAATGAGA CGATCTTGTT CAAAGAGAAA TTCCTTGATT GGACGGAACT GAAGAGACCT 
AACGAGAAGA ACGCCAGCGA ACTCGCGCAG CACAAGGACG ATGCCCGGGC AGAGGTCAAG 
CCTTACGACG TGACGCGGAT GGTGCCCGTG CCCCAGACAA CAGCCGGCAC CGTGCTGGAT 
GGGGTCAACG TGGGCCGAGG CTACGGGCTG GTGGAAGGGG ACGACCGGAG GCAGTTCGAG 
ATCGCCAGCA TCTCAGTGGA CGTCTGGCAC ATCCTGGAGT TCGACTACAG CAGGCTCCCC 
AAGCAGAGCA TCGGGCAGTT CCACGAAGGC GATGCCTACG TGGTGAAGTG GAAGTTCATC 
GTGAGCACTG CAGTGGGAAG CCGGCAGAAG GGGGAGCACT CGGTCAGGGT GGCTGGCAAA 
GAGAAATGTG TCTACTTCTT CTGGCAAGGC CGCCAGTCGA CCGTGAGTGA GAAGGGCACA 
TCAGCTCTGA TGACAGTGGA GCTGGACGAG GAAAGGGGGG CCCAGGTCCA GGTCCTGCAG 
GGCAAGGAGC CCCCCTGTTT CCTGCAGTGC TTCCAGGGGG GGATGGTGGT CCACTCCGGG 
AGACGGGAGG AGGAGGAAGA GAACACACAG AGCGAGTGGC GGCTGTACTG TGTGCGCGGA 
GAGGTGCCTG TGGAGGGGAA CTTGCTGGAG GTGGCCTGTC ACTGCAGCAG CCTCAGGTCT 
AGGACGTCCA TGGTTGTCCT CAACGTCCAC AAAGCCCTCA TCTACCTGTG GCATGGATGC 
AAAGCCCAGG CCCACACGAA GGAGGTCGGA CGGACTGCAG CCAATAAAAT CAAGGACCAA 
TGTCCCCTGG AGGCCGGGCT GCACAGTAGC AGCAAAGTGA CAATTCATGA GTGTGATGAA 
GGCTCGGAGC CCCTGGGGTT CTGGGACGCT CTGGGGAGGA GAGACCGGAA AGCCTACGAC 
TGCATGCTTC AAGATCCTGG AAATTTTAAC TTCACACCCC GCCTGTTCAT CCTCAGCAGC 
TCCTCTGGTG ACTTCTCAGC CACAGAGTTC ATGTATCCCG CCCGAGACCC CTCTGTGGTC 
AACTCTATGC CCTTCCTGCA GGAAGACCTA TACAGCGCCC CACAGCCAGC ACTCTTCCTT 
GTTGACAATC ACCACGAGGT GTACCTCTGG CAAGGCTGGT GGCCCATCGA GAACAAGATC 
ACGGGCTCCG CGCGCATCCG CTGGGCCTCG GACCGGAAGA GCGCCATGGA GACAGTGCTG 
CAGTACTGCC GAGGGAAAAA CCTGAAGAAG CCGCCCCCCA AGTCTTACCT CATCCACGCT 
GGCCTGGAAC CCCTGACCTT CACCAACATG TTCCCCAGCT GGGAGCACAG AGAAGACATT 
GCAGAGATCA CGGAGATGGA CACGGAAGTT TCAAATCAGA TCACCCTAGT GGAGGATGTC 
TTAGCCAAAC TGTGTAAGAC CATTTACCCA CTGGCCGACC TCCTAGCCAG GCCGCTCCCT 
GAGGGAGTTG ACCCTCTGAA GCTGGAGATC TATCTCACCG ACGAGGACTT CGAGTTTGCA 
CTAGACATGA CCCGAGACGA GTACAATGCA CTGCCCGCTT GGAAGCAGGT GAACCTGAAG 
AAAGCGAAAG GTCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000266.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856034.1 Bos taurus supervillin (SVIL), transcript variant X4, mRNA.

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CloneID	OBa221407
Clone ID Related Accession (Same CDS sequence)	XM_025000266.1
Accession Version	XM_025000266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTCCATTC TCGGGTTGAG GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG 
GAGAGAGGGT CCCGGAAACC GAGGCGCTAT TTTTCTCCTG GTGAAAATAG AAAGACTTCC 
GAGAGATTTA GAACTCAACC TATAACCTCG GCAGAACGAA AGGAATCGGA TAGGTCAACT 
TCAAATTCAG AAATGCCAGC TGCCGAGGAT GAAGAAAAGG TCGATGAGCG AGCGAGGCTG 
AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG GAGATGGAGA AGTCGTTTGA CGAGAAGAGC 
GTCCCAAAGC GCCGCTCCCG CAATGCGGCC GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC 
CGGTCCCACA CGCAGCCCGT CACCACCGAG GAGGTGGTCA TTGCAGCCAC TGAACCTACC 
CCCGCTTCGT GCTCAGTGGC CACCCACCCT GTAATGACAA GACATCCTAG CCCCACTGTA 
GCTAAGAGTC CCGTGCAGCC TGCCAGATTG CAGGCGTCTG CTCACCAAAA GGCACTAGCC 
AGAGACCAGA CAAACGAGAG CAAAGATTCT GCTGAGCAGG GAGAACCTGA CTCCTCCACT 
CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT AACAAACTAT CTCAGCCGGT CTCAAAAGCC 
ATTTCAACAC GGAACAGATT AGACATGAGA CAGAGGAGGA TGAATGCTCG TTATCAAACC 
CAGCCGGTCA CGCTTGGAGA AGTGGAACAG GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT 
CCCACCATTA ACACTTCCGT GTCCACCGTG GCATCTACCG TCCCCCCCAT GTATGCAGGA 
AATCTTCGGA CAAAGCCACT TCCAGATGAC AGCTTTGGTG CCACTGAGCA GAAGTTTGCT 
TCTTCGCTAG AAAACTCCGA CTCCCCAGTT AGAAGCATCC TGAAATCCCA AGGCTGGCAG 
CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA ATGTTGAGAG AATTTGAAGA GACAGAACGC 
AAGGGAGGCT TGACAGGTGG AGATGGCGGG GTTACAAAGT ATGGGTCCTT CGAGGAAGCA 
GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC CGGGAGGGAG ACAACCATAA AGAGGCCATC 
TATGCCCTTC CGAGGAAAGG AAGCCTGGAG CTCGCCCATC CTCCCATCGC CCAGCTTGGT 
GATGACCTGA AGGAATTTTC CACCCCAAAG AGCACCATGC AGGCAAGCCC AGACTGGAAG 
GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC TTGGAGAATG TCACAAAGAG GAAGTTTTCG 
CTGAAAGCCG CGGAGTTCGG GGAACCCACT TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA 
CCGGCTGCTC CGACTGCAAC CCCGGTGTCC TGGAAGCCGC AGGATCCCTC CGAACAGCCT 
CAGGAGAAGC GGTATCAGAG CCCGTGTGCG ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG 
GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC AAAACCATGT CCATTAAAGA AAGATTAGCA 
CTGTTGAAGA AGAGCGGTGA GGAAGACTGG AGAAACAGAC TCAACAGAAA GCAGGAATAC 
GGCAAAGCCT CCATCACCAG CAGCCTGCAC ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG 
AAGGAAGAAG GAGGATTTAC AGATGATAAT GACATTTCTA ATCTGCTTTG GCGGGTCACA 
GAAAGTCGAG AGAGCCAGAT GACTATTGAA GAGAGAAAGC ATCTCATCAC CGTGAGAGAG 
GACGCCTGGA AGACCAGGGG CAAAGGAGCA GCCAACGACT CTACCCAGTT TACTGTGGCC 
GGCAGGATGG TGAAGCGAGG TCTGGCGTCC CCCACTGCCA TCACCCCGGT AGCATCCCCT 
GTTTCCAGCA AAGCAAGGGG CACTACACCA GTTTCCAGAC CCCTGGAAGA TATCGAAGCC 
AGACCAGACA TGCAGTTAGA GTCGGACCTC AAGCTGGACA GGCTGGAAAC CTTCCTAAGA 
AGGCTGAATA ACAAAGTTGG TGGGATGCAA GAAACAGTCC TCACTGTCAC TGGGAAATCG 
GTGAAAGAGG TGATGAAGCC GGACGATGAT GAAACCTTTG CCAAGTTTTA CCGCAGCGTG 
GACTCCTCTC TACCGAGAAG CCCCGTAGAG CTGGACGAGG ATTTCGATGT CATTTTTGAT 
CCTTATGCCC CCAGGTTGAC ATCCTCGGTG GCTGAGCACA AGCGTGCCGT GCGGCCCAAG 
CGCCGGGTCC AGGCTTCCAA AAACCCCCTG AAGATGCTGG CCGCGAGGGA AGATCTCCTG 
CAGGAGTACA CTGAGCAGAG ACTGAACGTG GCCTTCGTGG AGTCAAAGCG GATGAAAGTC 
GAAAAGTTGT CCGCCAACTC CAGCTTCTCA GAAGTCACCC TGGCGGGGTT AGCCAGCAAA 
GAAAACTTCA GCAACGTCAG CCTGCGGAGC GTCAACCTGA CGGAACAGAA TTCCAACAAC 
AGTGCAGTGC CCTACAAGAA GCTGATGCTG CTGCAGGTTA AAGGAAGAAG ACACGTGCAG 
ACGCGGCTGG TGGAGCCTCG CGCCCCCTCC CTCAACAGCG GGGACTGCTT CCTCCTGCTC 
TCGCCCCATC ACTGCTTCCT GTGGGTCGGA GAGTTCGCCA ACGTGATCGA GAAGGCGAAG 
GCCTCAGAAC TTGCGAGTTT AATTCAGACC AAGAGGGAAC TTGGTTGTAG AGCAACTTAC 
ATCCAGACTG TTGAAGAAGG AATTAATACA CACACACATG CAGCCAAAGA CTTCTGGAAG 
CTTCTGGGCG GCCAAGCCAG TTACCAATCC GCCGGAGACC CAAAGGAGGA TGAGCTCTAT 
GAAACGGCCA TAATAGAGAC CAACTGCATT TACCGTCTGA TGGATGACAA ACTCGTTCCT 
GATGATGACT ACTGGGGGAA GATCCCAAAG TGCTCCCTCC TGCAATCAAA AGAGGTACTG 
GTGTTTGATT TTGGGAGTGA AGTTTACGTG TGGCATGGAA AAGAAGTCAC ATTAGCACAA 
CGGAAAATCG CCTTTCAGCT GGCAAAGCAC TTGTGGAATG GAACCTTTGA CTACGAGAAT 
TGTGACATTA ACCCGCTGGA TCCTGGGGAA TGCAATCCCC TCATTCCCAG GAAAGGACAG 
GGGCGGCCCG ACTGGGCAAT ATTTGGGAGA CTTACAGAAC ACAATGAGAC GATCTTGTTC 
AAAGAGAAAT TCCTTGATTG GACGGAACTG AAGAGACCTA ACGAGAAGAA CGCCAGCGAA 
CTCGCGCAGC ACAAGGACGA TGCCCGGGCA GAGGTCAAGC CTTACGACGT GACGCGGATG 
GTGCCCGTGC CCCAGACAAC AGCCGGCACC GTGCTGGATG GGGTCAACGT GGGCCGAGGC 
TACGGGCTGG TGGAAGGGGA CGACCGGAGG CAGTTCGAGA TCGCCAGCAT CTCAGTGGAC 
GTCTGGCACA TCCTGGAGTT CGACTACAGC AGGCTCCCCA AGCAGAGCAT CGGGCAGTTC 
CACGAAGGCG ATGCCTACGT GGTGAAGTGG AAGTTCATCG TGAGCACTGC AGTGGGAAGC 
CGGCAGAAGG GGGAGCACTC GGTCAGGGTG GCTGGCAAAG AGAAATGTGT CTACTTCTTC 
TGGCAAGGCC GCCAGTCGAC CGTGAGTGAG AAGGGCACAT CAGCTCTGAT GACAGTGGAG 
CTGGACGAGG AAAGGGGGGC CCAGGTCCAG GTCCTGCAGG GCAAGGAGCC CCCCTGTTTC 
CTGCAGTGCT TCCAGGGGGG GATGGTGGTC CACTCCGGGA GACGGGAGGA GGAGGAAGAG 
AACACACAGA GCGAGTGGCG GCTGTACTGT GTGCGCGGAG AGGTGCCTGT GGAGGGGAAC 
TTGCTGGAGG TGGCCTGTCA CTGCAGCAGC CTCAGGTCTA GGACGTCCAT GGTTGTCCTC 
AACGTCCACA AAGCCCTCAT CTACCTGTGG CATGGATGCA AAGCCCAGGC CCACACGAAG 
GAGGTCGGAC GGACTGCAGC CAATAAAATC AAGGACCAAT GTCCCCTGGA GGCCGGGCTG 
CACAGTAGCA GCAAAGTGAC AATTCATGAG TGTGATGAAG GCTCGGAGCC CCTGGGGTTC 
TGGGACGCTC TGGGGAGGAG AGACCGGAAA GCCTACGACT GCATGCTTCA AGATCCTGGA 
AATTTTAACT TCACACCCCG CCTGTTCATC CTCAGCAGCT CCTCTGGTGA CTTCTCAGCC 
ACAGAGTTCA TGTATCCCGC CCGAGACCCC TCTGTGGTCA ACTCTATGCC CTTCCTGCAG 
GAAGACCTAT ACAGCGCCCC ACAGCCAGCA CTCTTCCTTG TTGACAATCA CCACGAGGTG 
TACCTCTGGC AAGGCTGGTG GCCCATCGAG AACAAGATCA CGGGCTCCGC GCGCATCCGC 
TGGGCCTCGG ACCGGAAGAG CGCCATGGAG ACAGTGCTGC AGTACTGCCG AGGGAAAAAC 
CTGAAGAAGC CGCCCCCCAA GTCTTACCTC ATCCACGCTG GCCTGGAACC CCTGACCTTC 
ACCAACATGT TCCCCAGCTG GGAGCACAGA GAAGACATTG CAGAGATCAC GGAGATGGAC 
ACGGAAGTTT CAAATCAGAT CACCCTAGTG GAGGATGTCT TAGCCAAACT GTGTAAGACC 
ATTTACCCAC TGGCCGACCT CCTAGCCAGG CCGCTCCCTG AGGGAGTTGA CCCTCTGAAG 
CTGGAGATCT ATCTCACCGA CGAGGACTTC GAGTTTGCAC TAGACATGAC CCGAGACGAG 
TACAATGCAC TGCCCGCTTG GAAGCAGGTG AACCTGAAGA AAGCGAAAGG TCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856034.1
CDS	356..7255
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000266.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000266.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTCCATTC TCGGGTTGAG GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG 
GAGAGAGGGT CCCGGAAACC GAGGCGCTAT TTTTCTCCTG GTGAAAATAG AAAGACTTCC 
GAGAGATTTA GAACTCAACC TATAACCTCG GCAGAACGAA AGGAATCGGA TAGGTCAACT 
TCAAATTCAG AAATGCCAGC TGCCGAGGAT GAAGAAAAGG TCGATGAGCG AGCGAGGCTG 
AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG GAGATGGAGA AGTCGTTTGA CGAGAAGAGC 
GTCCCAAAGC GCCGCTCCCG CAATGCGGCC GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC 
CGGTCCCACA CGCAGCCCGT CACCACCGAG GAGGTGGTCA TTGCAGCCAC TGAACCTACC 
CCCGCTTCGT GCTCAGTGGC CACCCACCCT GTAATGACAA GACATCCTAG CCCCACTGTA 
GCTAAGAGTC CCGTGCAGCC TGCCAGATTG CAGGCGTCTG CTCACCAAAA GGCACTAGCC 
AGAGACCAGA CAAACGAGAG CAAAGATTCT GCTGAGCAGG GAGAACCTGA CTCCTCCACT 
CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT AACAAACTAT CTCAGCCGGT CTCAAAAGCC 
ATTTCAACAC GGAACAGATT AGACATGAGA CAGAGGAGGA TGAATGCTCG TTATCAAACC 
CAGCCGGTCA CGCTTGGAGA AGTGGAACAG GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT 
CCCACCATTA ACACTTCCGT GTCCACCGTG GCATCTACCG TCCCCCCCAT GTATGCAGGA 
AATCTTCGGA CAAAGCCACT TCCAGATGAC AGCTTTGGTG CCACTGAGCA GAAGTTTGCT 
TCTTCGCTAG AAAACTCCGA CTCCCCAGTT AGAAGCATCC TGAAATCCCA AGGCTGGCAG 
CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA ATGTTGAGAG AATTTGAAGA GACAGAACGC 
AAGGGAGGCT TGACAGGTGG AGATGGCGGG GTTACAAAGT ATGGGTCCTT CGAGGAAGCA 
GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC CGGGAGGGAG ACAACCATAA AGAGGCCATC 
TATGCCCTTC CGAGGAAAGG AAGCCTGGAG CTCGCCCATC CTCCCATCGC CCAGCTTGGT 
GATGACCTGA AGGAATTTTC CACCCCAAAG AGCACCATGC AGGCAAGCCC AGACTGGAAG 
GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC TTGGAGAATG TCACAAAGAG GAAGTTTTCG 
CTGAAAGCCG CGGAGTTCGG GGAACCCACT TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA 
CCGGCTGCTC CGACTGCAAC CCCGGTGTCC TGGAAGCCGC AGGATCCCTC CGAACAGCCT 
CAGGAGAAGC GGTATCAGAG CCCGTGTGCG ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG 
GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC AAAACCATGT CCATTAAAGA AAGATTAGCA 
CTGTTGAAGA AGAGCGGTGA GGAAGACTGG AGAAACAGAC TCAACAGAAA GCAGGAATAC 
GGCAAAGCCT CCATCACCAG CAGCCTGCAC ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG 
AAGGAAGAAG GAGGATTTAC AGATGATAAT GACATTTCTA ATCTGCTTTG GCGGGTCACA 
GAAAGTCGAG AGAGCCAGAT GACTATTGAA GAGAGAAAGC ATCTCATCAC CGTGAGAGAG 
GACGCCTGGA AGACCAGGGG CAAAGGAGCA GCCAACGACT CTACCCAGTT TACTGTGGCC 
GGCAGGATGG TGAAGCGAGG TCTGGCGTCC CCCACTGCCA TCACCCCGGT AGCATCCCCT 
GTTTCCAGCA AAGCAAGGGG CACTACACCA GTTTCCAGAC CCCTGGAAGA TATCGAAGCC 
AGACCAGACA TGCAGTTAGA GTCGGACCTC AAGCTGGACA GGCTGGAAAC CTTCCTAAGA 
AGGCTGAATA ACAAAGTTGG TGGGATGCAA GAAACAGTCC TCACTGTCAC TGGGAAATCG 
GTGAAAGAGG TGATGAAGCC GGACGATGAT GAAACCTTTG CCAAGTTTTA CCGCAGCGTG 
GACTCCTCTC TACCGAGAAG CCCCGTAGAG CTGGACGAGG ATTTCGATGT CATTTTTGAT 
CCTTATGCCC CCAGGTTGAC ATCCTCGGTG GCTGAGCACA AGCGTGCCGT GCGGCCCAAG 
CGCCGGGTCC AGGCTTCCAA AAACCCCCTG AAGATGCTGG CCGCGAGGGA AGATCTCCTG 
CAGGAGTACA CTGAGCAGAG ACTGAACGTG GCCTTCGTGG AGTCAAAGCG GATGAAAGTC 
GAAAAGTTGT CCGCCAACTC CAGCTTCTCA GAAGTCACCC TGGCGGGGTT AGCCAGCAAA 
GAAAACTTCA GCAACGTCAG CCTGCGGAGC GTCAACCTGA CGGAACAGAA TTCCAACAAC 
AGTGCAGTGC CCTACAAGAA GCTGATGCTG CTGCAGGTTA AAGGAAGAAG ACACGTGCAG 
ACGCGGCTGG TGGAGCCTCG CGCCCCCTCC CTCAACAGCG GGGACTGCTT CCTCCTGCTC 
TCGCCCCATC ACTGCTTCCT GTGGGTCGGA GAGTTCGCCA ACGTGATCGA GAAGGCGAAG 
GCCTCAGAAC TTGCGAGTTT AATTCAGACC AAGAGGGAAC TTGGTTGTAG AGCAACTTAC 
ATCCAGACTG TTGAAGAAGG AATTAATACA CACACACATG CAGCCAAAGA CTTCTGGAAG 
CTTCTGGGCG GCCAAGCCAG TTACCAATCC GCCGGAGACC CAAAGGAGGA TGAGCTCTAT 
GAAACGGCCA TAATAGAGAC CAACTGCATT TACCGTCTGA TGGATGACAA ACTCGTTCCT 
GATGATGACT ACTGGGGGAA GATCCCAAAG TGCTCCCTCC TGCAATCAAA AGAGGTACTG 
GTGTTTGATT TTGGGAGTGA AGTTTACGTG TGGCATGGAA AAGAAGTCAC ATTAGCACAA 
CGGAAAATCG CCTTTCAGCT GGCAAAGCAC TTGTGGAATG GAACCTTTGA CTACGAGAAT 
TGTGACATTA ACCCGCTGGA TCCTGGGGAA TGCAATCCCC TCATTCCCAG GAAAGGACAG 
GGGCGGCCCG ACTGGGCAAT ATTTGGGAGA CTTACAGAAC ACAATGAGAC GATCTTGTTC 
AAAGAGAAAT TCCTTGATTG GACGGAACTG AAGAGACCTA ACGAGAAGAA CGCCAGCGAA 
CTCGCGCAGC ACAAGGACGA TGCCCGGGCA GAGGTCAAGC CTTACGACGT GACGCGGATG 
GTGCCCGTGC CCCAGACAAC AGCCGGCACC GTGCTGGATG GGGTCAACGT GGGCCGAGGC 
TACGGGCTGG TGGAAGGGGA CGACCGGAGG CAGTTCGAGA TCGCCAGCAT CTCAGTGGAC 
GTCTGGCACA TCCTGGAGTT CGACTACAGC AGGCTCCCCA AGCAGAGCAT CGGGCAGTTC 
CACGAAGGCG ATGCCTACGT GGTGAAGTGG AAGTTCATCG TGAGCACTGC AGTGGGAAGC 
CGGCAGAAGG GGGAGCACTC GGTCAGGGTG GCTGGCAAAG AGAAATGTGT CTACTTCTTC 
TGGCAAGGCC GCCAGTCGAC CGTGAGTGAG AAGGGCACAT CAGCTCTGAT GACAGTGGAG 
CTGGACGAGG AAAGGGGGGC CCAGGTCCAG GTCCTGCAGG GCAAGGAGCC CCCCTGTTTC 
CTGCAGTGCT TCCAGGGGGG GATGGTGGTC CACTCCGGGA GACGGGAGGA GGAGGAAGAG 
AACACACAGA GCGAGTGGCG GCTGTACTGT GTGCGCGGAG AGGTGCCTGT GGAGGGGAAC 
TTGCTGGAGG TGGCCTGTCA CTGCAGCAGC CTCAGGTCTA GGACGTCCAT GGTTGTCCTC 
AACGTCCACA AAGCCCTCAT CTACCTGTGG CATGGATGCA AAGCCCAGGC CCACACGAAG 
GAGGTCGGAC GGACTGCAGC CAATAAAATC AAGGACCAAT GTCCCCTGGA GGCCGGGCTG 
CACAGTAGCA GCAAAGTGAC AATTCATGAG TGTGATGAAG GCTCGGAGCC CCTGGGGTTC 
TGGGACGCTC TGGGGAGGAG AGACCGGAAA GCCTACGACT GCATGCTTCA AGATCCTGGA 
AATTTTAACT TCACACCCCG CCTGTTCATC CTCAGCAGCT CCTCTGGTGA CTTCTCAGCC 
ACAGAGTTCA TGTATCCCGC CCGAGACCCC TCTGTGGTCA ACTCTATGCC CTTCCTGCAG 
GAAGACCTAT ACAGCGCCCC ACAGCCAGCA CTCTTCCTTG TTGACAATCA CCACGAGGTG 
TACCTCTGGC AAGGCTGGTG GCCCATCGAG AACAAGATCA CGGGCTCCGC GCGCATCCGC 
TGGGCCTCGG ACCGGAAGAG CGCCATGGAG ACAGTGCTGC AGTACTGCCG AGGGAAAAAC 
CTGAAGAAGC CGCCCCCCAA GTCTTACCTC ATCCACGCTG GCCTGGAACC CCTGACCTTC 
ACCAACATGT TCCCCAGCTG GGAGCACAGA GAAGACATTG CAGAGATCAC GGAGATGGAC 
ACGGAAGTTT CAAATCAGAT CACCCTAGTG GAGGATGTCT TAGCCAAACT GTGTAAGACC 
ATTTACCCAC TGGCCGACCT CCTAGCCAGG CCGCTCCCTG AGGGAGTTGA CCCTCTGAAG 
CTGGAGATCT ATCTCACCGA CGAGGACTTC GAGTTTGCAC TAGACATGAC CCGAGACGAG 
TACAATGCAC TGCCCGCTTG GAAGCAGGTG AACCTGAAGA AAGCGAAAGG TCTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000267.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856035.1 Bos taurus supervillin (SVIL), transcript variant X5, mRNA.

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CloneID	OBa221408
Clone ID Related Accession (Same CDS sequence)	XM_025000267.1
Accession Version	XM_025000267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTCCATTC TCGGGTTGAG GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG 
GAGAGAGGGT CCCGGAAACC GAGGCGCTAT TTTTCTCCTG GTGAAAATAG AAAGACTTCC 
GAGAGATTTA GAACTCAACC TATAACCTCG GCAGAACGAA AGGAATCGGA TAGGTCAACT 
TCAAATTCAG AAATGCCAGC TGCCGAGGAT GAAGAAAAGG TCGATGAGCG AGCGAGGCTG 
AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG GAGATGGAGA AGTCGTTTGA CGAGAAGAGC 
GTCCCAAAGC GCCGCTCCCG CAATGCGGCC GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC 
CGGTCCCACA CGCAGCCCGT CACCACCGAG GAGGTGGTCA TTGCAGCCAC ATTGCAGGCG 
TCTGCTCACC AAAAGGCACT AGCCAGAGAC CAGACAAACG AGAGCAAAGA TTCTGCTGAG 
CAGGGAGAAC CTGACTCCTC CACTCTGAGC TTAGCGGAGA AGCTGGCCTT GTTTAACAAA 
CTATCTCAGC CGGTCTCAAA AGCCATTTCA ACACGGAACA GATTAGACAT GAGACAGAGG 
AGGATGAATG CTCGTTATCA AACCCAGCCG GTCACGCTTG GAGAAGTGGA ACAGGTGCAG 
AGTGGCAAGC TCATGGCTTT CTCTCCCACC ATTAACACTT CCGTGTCCAC CGTGGCATCT 
ACCGTCCCCC CCATGTATGC AGGAAATCTT CGGACAAAGC CACTTCCAGA TGACAGCTTT 
GGTGCCACTG AGCAGAAGTT TGCTTCTTCG CTAGAAAACT CCGACTCCCC AGTTAGAAGC 
ATCCTGAAAT CCCAAGGCTG GCAGCCCTCG GTCGAGGGTG CTGGGAGCAA AGCAATGTTG 
AGAGAATTTG AAGAGACAGA ACGCAAGGGA GGCTTGACAG GTGGAGATGG CGGGGTTACA 
AAGTATGGGT CCTTCGAGGA AGCAGAGCTG TCCTACCCCG TCCTCAGCAG AGTCCGGGAG 
GGAGACAACC ATAAAGAGGC CATCTATGCC CTTCCGAGGA AAGGAAGCCT GGAGCTCGCC 
CATCCTCCCA TCGCCCAGCT TGGTGATGAC CTGAAGGAAT TTTCCACCCC AAAGAGCACC 
ATGCAGGCAA GCCCAGACTG GAAGGAGAGG CAGCTCTTTG AAGAGAAGGT GGACTTGGAG 
AATGTCACAA AGAGGAAGTT TTCGCTGAAA GCCGCGGAGT TCGGGGAACC CACTTCTGAG 
CAGACCGGCG CGGCTGCTGG GAAACCGGCT GCTCCGACTG CAACCCCGGT GTCCTGGAAG 
CCGCAGGATC CCTCCGAACA GCCTCAGGAG AAGCGGTATC AGAGCCCGTG TGCGATGTTT 
GCTGCTGGAG AGATCAAAGC CCCGGCGGTG GAGGGCAGCC TCGACTCCCC CAGCAAAACC 
ATGTCCATTA AAGAAAGATT AGCACTGTTG AAGAAGAGCG GTGAGGAAGA CTGGAGAAAC 
AGACTCAACA GAAAGCAGGA ATACGGCAAA GCCTCCATCA CCAGCAGCCT GCACATCCAA 
GAGACGGAGC AGTCACTCAA GAAGAAGGAA GAAGGAGGAT TTACAGATGA TAATGACATT 
TCTAATCTGC TTTGGGAACC TGTATACGCT TCTACTTATT CTCCTGCTCT ACCTGCTGCC 
CATAAATACC TGTCTTTTGT ATCTATTAAT CAGCGGGTCA CAGAAAGTCG AGAGAGCCAG 
ATGACTATTG AAGAGAGAAA GCATCTCATC ACCGTGAGAG AGGACGCCTG GAAGACCAGG 
GGCAAAGGAG CAGCCAACGA CTCTACCCAG TTTACTGTGG CCGGCAGGAT GGTGAAGCGA 
GGTCTGGCGT CCCCCACTGC CATCACCCCG GTAGCATCCC CTGTTTCCAG CAAAGCAAGG 
GGCACTACAC CAGTTTCCAG ACCCCTGGAA GATATCGAAG CCAGACCAGA CATGCAGTTA 
GAGTCGGACC TCAAGCTGGA CAGGCTGGAA ACCTTCCTAA GAAGGCTGAA TAACAAAGTT 
GGTGGGATGC AAGAAACAGT CCTCACTGTC ACTGGGAAAT CGGTGAAAGA GGTGATGAAG 
CCGGACGATG ATGAAACCTT TGCCAAGTTT TACCGCAGCG TGGACTCCTC TCTACCGAGA 
AGCCCCGTAG AGCTGGACGA GGATTTCGAT GTCATTTTTG ATCCTTATGC CCCCAGGTTG 
ACATCCTCGG TGGCTGAGCA CAAGCGTGCC GTGCGGCCCA AGCGCCGGGT CCAGGCTTCC 
AAAAACCCCC TGAAGATGCT GGCCGCGAGG GAAGATCTCC TGCAGGAGTA CACTGAGCAG 
AGACTGAACG TGGCCTTCGT GGAGTCAAAG CGGATGAAAG TCGAAAAGTT GTCCGCCAAC 
TCCAGCTTCT CAGAAGTCAC CCTGGCGGGG TTAGCCAGCA AAGAAAACTT CAGCAACGTC 
AGCCTGCGGA GCGTCAACCT GACGGAACAG AATTCCAACA ACAGTGCAGT GCCCTACAAG 
AAGCTGATGC TGCTGCAGGT TAAAGGAAGA AGACACGTGC AGACGCGGCT GGTGGAGCCT 
CGCGCCCCCT CCCTCAACAG CGGGGACTGC TTCCTCCTGC TCTCGCCCCA TCACTGCTTC 
CTGTGGGTCG GAGAGTTCGC CAACGTGATC GAGAAGGCGA AGGCCTCAGA ACTTGCGAGT 
TTAATTCAGA CCAAGAGGGA ACTTGGTTGT AGAGCAACTT ACATCCAGAC TGTTGAAGAA 
GGAATTAATA CACACACACA TGCAGCCAAA GACTTCTGGA AGCTTCTGGG CGGCCAAGCC 
AGTTACCAAT CCGCCGGAGA CCCAAAGGAG GATGAGCTCT ATGAAACGGC CATAATAGAG 
ACCAACTGCA TTTACCGTCT GATGGATGAC AAACTCGTTC CTGATGATGA CTACTGGGGG 
AAGATCCCAA AGTGCTCCCT CCTGCAATCA AAAGAGGTAC TGGTGTTTGA TTTTGGGAGT 
GAAGTTTACG TGTGGCATGG AAAAGAAGTC ACATTAGCAC AACGGAAAAT CGCCTTTCAG 
CTGGCAAAGC ACTTGTGGAA TGGAACCTTT GACTACGAGA ATTGTGACAT TAACCCGCTG 
GATCCTGGGG AATGCAATCC CCTCATTCCC AGGAAAGGAC AGGGGCGGCC CGACTGGGCA 
ATATTTGGGA GACTTACAGA ACACAATGAG ACGATCTTGT TCAAAGAGAA ATTCCTTGAT 
TGGACGGAAC TGAAGAGACC TAACGAGAAG AACGCCAGCG AACTCGCGCA GCACAAGGAC 
GATGCCCGGG CAGAGGTCAA GCCTTACGAC GTGACGCGGA TGGTGCCCGT GCCCCAGACA 
ACAGCCGGCA CCGTGCTGGA TGGGGTCAAC GTGGGCCGAG GCTACGGGCT GGTGGAAGGG 
GACGACCGGA GGCAGTTCGA GATCGCCAGC ATCTCAGTGG ACGTCTGGCA CATCCTGGAG 
TTCGACTACA GCAGGCTCCC CAAGCAGAGC ATCGGGCAGT TCCACGAAGG CGATGCCTAC 
GTGGTGAAGT GGAAGTTCAT CGTGAGCACT GCAGTGGGAA GCCGGCAGAA GGGGGAGCAC 
TCGGTCAGGG TGGCTGGCAA AGAGAAATGT GTCTACTTCT TCTGGCAAGG CCGCCAGTCG 
ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG ATGACAGTGG AGCTGGACGA GGAAAGGGGG 
GCCCAGGTCC AGGTCCTGCA GGGCAAGGAG CCCCCCTGTT TCCTGCAGTG CTTCCAGGGG 
GGGATGGTGG TCCACTCCGG GAGACGGGAG GAGGAGGAAG AGAACACACA GAGCGAGTGG 
CGGCTGTACT GTGTGCGCGG AGAGGTGCCT GTGGAGGGGA ACTTGCTGGA GGTGGCCTGT 
CACTGCAGCA GCCTCAGGTC TAGGACGTCC ATGGTTGTCC TCAACGTCCA CAAAGCCCTC 
ATCTACCTGT GGCATGGATG CAAAGCCCAG GCCCACACGA AGGAGGTCGG ACGGACTGCA 
GCCAATAAAA TCAAGGACCA ATGTCCCCTG GAGGCCGGGC TGCACAGTAG CAGCAAAGTG 
ACAATTCATG AGTGTGATGA AGGCTCGGAG CCCCTGGGGT TCTGGGACGC TCTGGGGAGG 
AGAGACCGGA AAGCCTACGA CTGCATGCTT CAAGATCCTG GAAATTTTAA CTTCACACCC 
CGCCTGTTCA TCCTCAGCAG CTCCTCTGGT GACTTCTCAG CCACAGAGTT CATGTATCCC 
GCCCGAGACC CCTCTGTGGT CAACTCTATG CCCTTCCTGC AGGAAGACCT ATACAGCGCC 
CCACAGCCAG CACTCTTCCT TGTTGACAAT CACCACGAGG TGTACCTCTG GCAAGGCTGG 
TGGCCCATCG AGAACAAGAT CACGGGCTCC GCGCGCATCC GCTGGGCCTC GGACCGGAAG 
AGCGCCATGG AGACAGTGCT GCAGTACTGC CGAGGGAAAA ACCTGAAGAA GCCGCCCCCC 
AAGTCTTACC TCATCCACGC TGGCCTGGAA CCCCTGACCT TCACCAACAT GTTCCCCAGC 
TGGGAGCACA GAGAAGACAT TGCAGAGATC ACGGAGATGG ACACGGAAGT TTCAAATCAG 
ATCACCCTAG TGGAGGATGT CTTAGCCAAA CTGTGTAAGA CCATTTACCC ACTGGCCGAC 
CTCCTAGCCA GGCCGCTCCC TGAGGGAGTT GACCCTCTGA AGCTGGAGAT CTATCTCACC 
GACGAGGACT TCGAGTTTGC ACTAGACATG ACCCGAGACG AGTACAATGC ACTGCCCGCT 
TGGAAGCAGG TGAACCTGAA GAAAGCGAAA GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856035.1
CDS	356..7237
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000267.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000267.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTCCATTC TCGGGTTGAG GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG 
GAGAGAGGGT CCCGGAAACC GAGGCGCTAT TTTTCTCCTG GTGAAAATAG AAAGACTTCC 
GAGAGATTTA GAACTCAACC TATAACCTCG GCAGAACGAA AGGAATCGGA TAGGTCAACT 
TCAAATTCAG AAATGCCAGC TGCCGAGGAT GAAGAAAAGG TCGATGAGCG AGCGAGGCTG 
AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG GAGATGGAGA AGTCGTTTGA CGAGAAGAGC 
GTCCCAAAGC GCCGCTCCCG CAATGCGGCC GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC 
CGGTCCCACA CGCAGCCCGT CACCACCGAG GAGGTGGTCA TTGCAGCCAC ATTGCAGGCG 
TCTGCTCACC AAAAGGCACT AGCCAGAGAC CAGACAAACG AGAGCAAAGA TTCTGCTGAG 
CAGGGAGAAC CTGACTCCTC CACTCTGAGC TTAGCGGAGA AGCTGGCCTT GTTTAACAAA 
CTATCTCAGC CGGTCTCAAA AGCCATTTCA ACACGGAACA GATTAGACAT GAGACAGAGG 
AGGATGAATG CTCGTTATCA AACCCAGCCG GTCACGCTTG GAGAAGTGGA ACAGGTGCAG 
AGTGGCAAGC TCATGGCTTT CTCTCCCACC ATTAACACTT CCGTGTCCAC CGTGGCATCT 
ACCGTCCCCC CCATGTATGC AGGAAATCTT CGGACAAAGC CACTTCCAGA TGACAGCTTT 
GGTGCCACTG AGCAGAAGTT TGCTTCTTCG CTAGAAAACT CCGACTCCCC AGTTAGAAGC 
ATCCTGAAAT CCCAAGGCTG GCAGCCCTCG GTCGAGGGTG CTGGGAGCAA AGCAATGTTG 
AGAGAATTTG AAGAGACAGA ACGCAAGGGA GGCTTGACAG GTGGAGATGG CGGGGTTACA 
AAGTATGGGT CCTTCGAGGA AGCAGAGCTG TCCTACCCCG TCCTCAGCAG AGTCCGGGAG 
GGAGACAACC ATAAAGAGGC CATCTATGCC CTTCCGAGGA AAGGAAGCCT GGAGCTCGCC 
CATCCTCCCA TCGCCCAGCT TGGTGATGAC CTGAAGGAAT TTTCCACCCC AAAGAGCACC 
ATGCAGGCAA GCCCAGACTG GAAGGAGAGG CAGCTCTTTG AAGAGAAGGT GGACTTGGAG 
AATGTCACAA AGAGGAAGTT TTCGCTGAAA GCCGCGGAGT TCGGGGAACC CACTTCTGAG 
CAGACCGGCG CGGCTGCTGG GAAACCGGCT GCTCCGACTG CAACCCCGGT GTCCTGGAAG 
CCGCAGGATC CCTCCGAACA GCCTCAGGAG AAGCGGTATC AGAGCCCGTG TGCGATGTTT 
GCTGCTGGAG AGATCAAAGC CCCGGCGGTG GAGGGCAGCC TCGACTCCCC CAGCAAAACC 
ATGTCCATTA AAGAAAGATT AGCACTGTTG AAGAAGAGCG GTGAGGAAGA CTGGAGAAAC 
AGACTCAACA GAAAGCAGGA ATACGGCAAA GCCTCCATCA CCAGCAGCCT GCACATCCAA 
GAGACGGAGC AGTCACTCAA GAAGAAGGAA GAAGGAGGAT TTACAGATGA TAATGACATT 
TCTAATCTGC TTTGGGAACC TGTATACGCT TCTACTTATT CTCCTGCTCT ACCTGCTGCC 
CATAAATACC TGTCTTTTGT ATCTATTAAT CAGCGGGTCA CAGAAAGTCG AGAGAGCCAG 
ATGACTATTG AAGAGAGAAA GCATCTCATC ACCGTGAGAG AGGACGCCTG GAAGACCAGG 
GGCAAAGGAG CAGCCAACGA CTCTACCCAG TTTACTGTGG CCGGCAGGAT GGTGAAGCGA 
GGTCTGGCGT CCCCCACTGC CATCACCCCG GTAGCATCCC CTGTTTCCAG CAAAGCAAGG 
GGCACTACAC CAGTTTCCAG ACCCCTGGAA GATATCGAAG CCAGACCAGA CATGCAGTTA 
GAGTCGGACC TCAAGCTGGA CAGGCTGGAA ACCTTCCTAA GAAGGCTGAA TAACAAAGTT 
GGTGGGATGC AAGAAACAGT CCTCACTGTC ACTGGGAAAT CGGTGAAAGA GGTGATGAAG 
CCGGACGATG ATGAAACCTT TGCCAAGTTT TACCGCAGCG TGGACTCCTC TCTACCGAGA 
AGCCCCGTAG AGCTGGACGA GGATTTCGAT GTCATTTTTG ATCCTTATGC CCCCAGGTTG 
ACATCCTCGG TGGCTGAGCA CAAGCGTGCC GTGCGGCCCA AGCGCCGGGT CCAGGCTTCC 
AAAAACCCCC TGAAGATGCT GGCCGCGAGG GAAGATCTCC TGCAGGAGTA CACTGAGCAG 
AGACTGAACG TGGCCTTCGT GGAGTCAAAG CGGATGAAAG TCGAAAAGTT GTCCGCCAAC 
TCCAGCTTCT CAGAAGTCAC CCTGGCGGGG TTAGCCAGCA AAGAAAACTT CAGCAACGTC 
AGCCTGCGGA GCGTCAACCT GACGGAACAG AATTCCAACA ACAGTGCAGT GCCCTACAAG 
AAGCTGATGC TGCTGCAGGT TAAAGGAAGA AGACACGTGC AGACGCGGCT GGTGGAGCCT 
CGCGCCCCCT CCCTCAACAG CGGGGACTGC TTCCTCCTGC TCTCGCCCCA TCACTGCTTC 
CTGTGGGTCG GAGAGTTCGC CAACGTGATC GAGAAGGCGA AGGCCTCAGA ACTTGCGAGT 
TTAATTCAGA CCAAGAGGGA ACTTGGTTGT AGAGCAACTT ACATCCAGAC TGTTGAAGAA 
GGAATTAATA CACACACACA TGCAGCCAAA GACTTCTGGA AGCTTCTGGG CGGCCAAGCC 
AGTTACCAAT CCGCCGGAGA CCCAAAGGAG GATGAGCTCT ATGAAACGGC CATAATAGAG 
ACCAACTGCA TTTACCGTCT GATGGATGAC AAACTCGTTC CTGATGATGA CTACTGGGGG 
AAGATCCCAA AGTGCTCCCT CCTGCAATCA AAAGAGGTAC TGGTGTTTGA TTTTGGGAGT 
GAAGTTTACG TGTGGCATGG AAAAGAAGTC ACATTAGCAC AACGGAAAAT CGCCTTTCAG 
CTGGCAAAGC ACTTGTGGAA TGGAACCTTT GACTACGAGA ATTGTGACAT TAACCCGCTG 
GATCCTGGGG AATGCAATCC CCTCATTCCC AGGAAAGGAC AGGGGCGGCC CGACTGGGCA 
ATATTTGGGA GACTTACAGA ACACAATGAG ACGATCTTGT TCAAAGAGAA ATTCCTTGAT 
TGGACGGAAC TGAAGAGACC TAACGAGAAG AACGCCAGCG AACTCGCGCA GCACAAGGAC 
GATGCCCGGG CAGAGGTCAA GCCTTACGAC GTGACGCGGA TGGTGCCCGT GCCCCAGACA 
ACAGCCGGCA CCGTGCTGGA TGGGGTCAAC GTGGGCCGAG GCTACGGGCT GGTGGAAGGG 
GACGACCGGA GGCAGTTCGA GATCGCCAGC ATCTCAGTGG ACGTCTGGCA CATCCTGGAG 
TTCGACTACA GCAGGCTCCC CAAGCAGAGC ATCGGGCAGT TCCACGAAGG CGATGCCTAC 
GTGGTGAAGT GGAAGTTCAT CGTGAGCACT GCAGTGGGAA GCCGGCAGAA GGGGGAGCAC 
TCGGTCAGGG TGGCTGGCAA AGAGAAATGT GTCTACTTCT TCTGGCAAGG CCGCCAGTCG 
ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG ATGACAGTGG AGCTGGACGA GGAAAGGGGG 
GCCCAGGTCC AGGTCCTGCA GGGCAAGGAG CCCCCCTGTT TCCTGCAGTG CTTCCAGGGG 
GGGATGGTGG TCCACTCCGG GAGACGGGAG GAGGAGGAAG AGAACACACA GAGCGAGTGG 
CGGCTGTACT GTGTGCGCGG AGAGGTGCCT GTGGAGGGGA ACTTGCTGGA GGTGGCCTGT 
CACTGCAGCA GCCTCAGGTC TAGGACGTCC ATGGTTGTCC TCAACGTCCA CAAAGCCCTC 
ATCTACCTGT GGCATGGATG CAAAGCCCAG GCCCACACGA AGGAGGTCGG ACGGACTGCA 
GCCAATAAAA TCAAGGACCA ATGTCCCCTG GAGGCCGGGC TGCACAGTAG CAGCAAAGTG 
ACAATTCATG AGTGTGATGA AGGCTCGGAG CCCCTGGGGT TCTGGGACGC TCTGGGGAGG 
AGAGACCGGA AAGCCTACGA CTGCATGCTT CAAGATCCTG GAAATTTTAA CTTCACACCC 
CGCCTGTTCA TCCTCAGCAG CTCCTCTGGT GACTTCTCAG CCACAGAGTT CATGTATCCC 
GCCCGAGACC CCTCTGTGGT CAACTCTATG CCCTTCCTGC AGGAAGACCT ATACAGCGCC 
CCACAGCCAG CACTCTTCCT TGTTGACAAT CACCACGAGG TGTACCTCTG GCAAGGCTGG 
TGGCCCATCG AGAACAAGAT CACGGGCTCC GCGCGCATCC GCTGGGCCTC GGACCGGAAG 
AGCGCCATGG AGACAGTGCT GCAGTACTGC CGAGGGAAAA ACCTGAAGAA GCCGCCCCCC 
AAGTCTTACC TCATCCACGC TGGCCTGGAA CCCCTGACCT TCACCAACAT GTTCCCCAGC 
TGGGAGCACA GAGAAGACAT TGCAGAGATC ACGGAGATGG ACACGGAAGT TTCAAATCAG 
ATCACCCTAG TGGAGGATGT CTTAGCCAAA CTGTGTAAGA CCATTTACCC ACTGGCCGAC 
CTCCTAGCCA GGCCGCTCCC TGAGGGAGTT GACCCTCTGA AGCTGGAGAT CTATCTCACC 
GACGAGGACT TCGAGTTTGC ACTAGACATG ACCCGAGACG AGTACAATGC ACTGCCCGCT 
TGGAAGCAGG TGAACCTGAA GAAAGCGAAA GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000268.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856036.1 Bos taurus supervillin (SVIL), transcript variant X6, mRNA.

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CloneID	OBa221409
Clone ID Related Accession (Same CDS sequence)	XM_025000268.1
Accession Version	XM_025000268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTCCATTC TCGGGTTGAG GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG 
GAGAGAGGGT CCCGGAAACC GAGGCGCTAT TTTTCTCCTG GTGAAAATAG AAAGACTTCC 
GAGAGATTTA GAACTCAACC TATAACCTCG GCAGAACGAA AGGAATCGGA TAGGTCAACT 
TCAAATTCAG AAATGCCAGC TGCCGAGGAT GAAGAAAAGG TCGATGAGCG AGCGAGGCTG 
AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG GAGATGGAGA AGTCGTTTGA CGAGAAGAGC 
GTCCCAAAGC GCCGCTCCCG CAATGCGGCC GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC 
CGGTCCCACA CGCAGCCCGT CACCACCGAG GAGGTGGTCA TTGCAGCCAC TGAACCTACC 
CCCGCTTCGT GCTCAGTGGC CACCCACCCT GTAATGACAA GACATCCTAG CCCCACTGTA 
GCTAAGAGTC CCGTGCAGCC TGCCAGATTG CAGGCGTCTG CTCACCAAAA GGCACTAGCC 
AGAGACCAGA CAAACGAGAG CAAAGATTCT GCTGAGCAGG GAGAACCTGA CTCCTCCACT 
CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT AACAAACTAT CTCAGCCGGT CTCAAAAGCC 
ATTTCAACAC GGAACAGATT AGACATGAGA CAGAGGAGGA TGAATGCTCG TTATCAAACC 
CAGCCGGTCA CGCTTGGAGA AGTGGAACAG GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT 
CCCACCATTA ACACTTCCGT GTCCACCGTG GCATCTACCG TCCCCCCCAT GTATGCAGGA 
AATCTTCGGA CAAAGCCACT TCCAGATGAC AGCTTTGGTG CCACTGAGCA GAAGTTTGCT 
TCTTCGCTAG AAAACTCCGA CTCCCCAGTT AGAAGCATCC TGAAATCCCA AGGCTGGCAG 
CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA ATGTTGAGAG AATTTGAAGA GACAGAACGC 
AAGGGAGGCT TGACAGGTGG AGATGGCGGG GTTACAAAGT ATGGGTCCTT CGAGGAAGCA 
GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC CGGGAGGGAG ACAACCATAA AGAGGCCATC 
TATGCCCTTC CGAGGAAAGG AAGCCTGGAG CTCGCCCATC CTCCCATCGC CCAGCTTGGT 
GATGACCTGA AGGAATTTTC CACCCCAAAG AGCACCATGC AGGCAAGCCC AGACTGGAAG 
GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC TTGGAGAATG TCACAAAGAG GAAGTTTTCG 
CTGAAAGCCG CGGAGTTCGG GGAACCCACT TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA 
CCGGCTGCTC CGACTGCAAC CCCGGTGTCC TGGAAGCCGC AGGATCCCTC CGAACAGCCT 
CAGGAGAAGC GGTATCAGAG CCCGTGTGCG ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG 
GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC AAAACCATGT CCATTAAAGA AAGATTAGCA 
CTGTTGAAGA AGAGCGGTGA GGAAGACTGG AGAAACAGAC TCAACAGAAA GCAGGAATAC 
GGCAAAGCCT CCATCACCAG CAGCCTGCAC ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG 
AAGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATTG AAGAGAGAAA GCATCTCATC 
ACCGTGAGAG AGGACGCCTG GAAGACCAGG GGCAAAGGAG CAGCCAACGA CTCTACCCAG 
TTTACTGTGG CCGGCAGGAT GGTGAAGCGA GGTCTGGCGT CCCCCACTGC CATCACCCCG 
GTAGCATCCC CTGTTTCCAG CAAAGCAAGG GGCACTACAC CAGTTTCCAG ACCCCTGGAA 
GATATCGAAG CCAGACCAGA CATGCAGTTA GAGTCGGACC TCAAGCTGGA CAGGCTGGAA 
ACCTTCCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AAGAAACAGT CCTCACTGTC 
ACTGGGAAAT CGGTGAAAGA GGTGATGAAG CCGGACGATG ATGAAACCTT TGCCAAGTTT 
TACCGCAGCG TGGACTCCTC TCTACCGAGA AGCCCCGTAG AGCTGGACGA GGATTTCGAT 
GTCATTTTTG ATCCTTATGC CCCCAGGTTG ACATCCTCGG TGGCTGAGCA CAAGCGTGCC 
GTGCGGCCCA AGCGCCGGGT CCAGGCTTCC AAAAACCCCC TGAAGATGCT GGCCGCGAGG 
GAAGATCTCC TGCAGGAGTA CACTGAGCAG AGACTGAACG TGGCCTTCGT GGAGTCAAAG 
CGGATGAAAG TCGAAAAGTT GTCCGCCAAC TCCAGCTTCT CAGAAGTCAC CCTGGCGGGG 
TTAGCCAGCA AAGAAAACTT CAGCAACGTC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ACAGTGCAGT GCCCTACAAG AAGCTGATGC TGCTGCAGGT TAAAGGAAGA 
AGACACGTGC AGACGCGGCT GGTGGAGCCT CGCGCCCCCT CCCTCAACAG CGGGGACTGC 
TTCCTCCTGC TCTCGCCCCA TCACTGCTTC CTGTGGGTCG GAGAGTTCGC CAACGTGATC 
GAGAAGGCGA AGGCCTCAGA ACTTGCGAGT TTAATTCAGA CCAAGAGGGA ACTTGGTTGT 
AGAGCAACTT ACATCCAGAC TGTTGAAGAA GGAATTAATA CACACACACA TGCAGCCAAA 
GACTTCTGGA AGCTTCTGGG CGGCCAAGCC AGTTACCAAT CCGCCGGAGA CCCAAAGGAG 
GATGAGCTCT ATGAAACGGC CATAATAGAG ACCAACTGCA TTTACCGTCT GATGGATGAC 
AAACTCGTTC CTGATGATGA CTACTGGGGG AAGATCCCAA AGTGCTCCCT CCTGCAATCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGGAGT GAAGTTTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCAC AACGGAAAAT CGCCTTTCAG CTGGCAAAGC ACTTGTGGAA TGGAACCTTT 
GACTACGAGA ATTGTGACAT TAACCCGCTG GATCCTGGGG AATGCAATCC CCTCATTCCC 
AGGAAAGGAC AGGGGCGGCC CGACTGGGCA ATATTTGGGA GACTTACAGA ACACAATGAG 
ACGATCTTGT TCAAAGAGAA ATTCCTTGAT TGGACGGAAC TGAAGAGACC TAACGAGAAG 
AACGCCAGCG AACTCGCGCA GCACAAGGAC GATGCCCGGG CAGAGGTCAA GCCTTACGAC 
GTGACGCGGA TGGTGCCCGT GCCCCAGACA ACAGCCGGCA CCGTGCTGGA TGGGGTCAAC 
GTGGGCCGAG GCTACGGGCT GGTGGAAGGG GACGACCGGA GGCAGTTCGA GATCGCCAGC 
ATCTCAGTGG ACGTCTGGCA CATCCTGGAG TTCGACTACA GCAGGCTCCC CAAGCAGAGC 
ATCGGGCAGT TCCACGAAGG CGATGCCTAC GTGGTGAAGT GGAAGTTCAT CGTGAGCACT 
GCAGTGGGAA GCCGGCAGAA GGGGGAGCAC TCGGTCAGGG TGGCTGGCAA AGAGAAATGT 
GTCTACTTCT TCTGGCAAGG CCGCCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGACGA GGAAAGGGGG GCCCAGGTCC AGGTCCTGCA GGGCAAGGAG 
CCCCCCTGTT TCCTGCAGTG CTTCCAGGGG GGGATGGTGG TCCACTCCGG GAGACGGGAG 
GAGGAGGAAG AGAACACACA GAGCGAGTGG CGGCTGTACT GTGTGCGCGG AGAGGTGCCT 
GTGGAGGGGA ACTTGCTGGA GGTGGCCTGT CACTGCAGCA GCCTCAGGTC TAGGACGTCC 
ATGGTTGTCC TCAACGTCCA CAAAGCCCTC ATCTACCTGT GGCATGGATG CAAAGCCCAG 
GCCCACACGA AGGAGGTCGG ACGGACTGCA GCCAATAAAA TCAAGGACCA ATGTCCCCTG 
GAGGCCGGGC TGCACAGTAG CAGCAAAGTG ACAATTCATG AGTGTGATGA AGGCTCGGAG 
CCCCTGGGGT TCTGGGACGC TCTGGGGAGG AGAGACCGGA AAGCCTACGA CTGCATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCC CGCCTGTTCA TCCTCAGCAG CTCCTCTGGT 
GACTTCTCAG CCACAGAGTT CATGTATCCC GCCCGAGACC CCTCTGTGGT CAACTCTATG 
CCCTTCCTGC AGGAAGACCT ATACAGCGCC CCACAGCCAG CACTCTTCCT TGTTGACAAT 
CACCACGAGG TGTACCTCTG GCAAGGCTGG TGGCCCATCG AGAACAAGAT CACGGGCTCC 
GCGCGCATCC GCTGGGCCTC GGACCGGAAG AGCGCCATGG AGACAGTGCT GCAGTACTGC 
CGAGGGAAAA ACCTGAAGAA GCCGCCCCCC AAGTCTTACC TCATCCACGC TGGCCTGGAA 
CCCCTGACCT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAAGACAT TGCAGAGATC 
ACGGAGATGG ACACGGAAGT TTCAAATCAG ATCACCCTAG TGGAGGATGT CTTAGCCAAA 
CTGTGTAAGA CCATTTACCC ACTGGCCGAC CTCCTAGCCA GGCCGCTCCC TGAGGGAGTT 
GACCCTCTGA AGCTGGAGAT CTATCTCACC GACGAGGACT TCGAGTTTGC ACTAGACATG 
ACCCGAGACG AGTACAATGC ACTGCCCGCT TGGAAGCAGG TGAACCTGAA GAAAGCGAAA 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856036.1
CDS	356..7207
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000268.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000268.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTCCATTC TCGGGTTGAG GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG 
GAGAGAGGGT CCCGGAAACC GAGGCGCTAT TTTTCTCCTG GTGAAAATAG AAAGACTTCC 
GAGAGATTTA GAACTCAACC TATAACCTCG GCAGAACGAA AGGAATCGGA TAGGTCAACT 
TCAAATTCAG AAATGCCAGC TGCCGAGGAT GAAGAAAAGG TCGATGAGCG AGCGAGGCTG 
AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG GAGATGGAGA AGTCGTTTGA CGAGAAGAGC 
GTCCCAAAGC GCCGCTCCCG CAATGCGGCC GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC 
CGGTCCCACA CGCAGCCCGT CACCACCGAG GAGGTGGTCA TTGCAGCCAC TGAACCTACC 
CCCGCTTCGT GCTCAGTGGC CACCCACCCT GTAATGACAA GACATCCTAG CCCCACTGTA 
GCTAAGAGTC CCGTGCAGCC TGCCAGATTG CAGGCGTCTG CTCACCAAAA GGCACTAGCC 
AGAGACCAGA CAAACGAGAG CAAAGATTCT GCTGAGCAGG GAGAACCTGA CTCCTCCACT 
CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT AACAAACTAT CTCAGCCGGT CTCAAAAGCC 
ATTTCAACAC GGAACAGATT AGACATGAGA CAGAGGAGGA TGAATGCTCG TTATCAAACC 
CAGCCGGTCA CGCTTGGAGA AGTGGAACAG GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT 
CCCACCATTA ACACTTCCGT GTCCACCGTG GCATCTACCG TCCCCCCCAT GTATGCAGGA 
AATCTTCGGA CAAAGCCACT TCCAGATGAC AGCTTTGGTG CCACTGAGCA GAAGTTTGCT 
TCTTCGCTAG AAAACTCCGA CTCCCCAGTT AGAAGCATCC TGAAATCCCA AGGCTGGCAG 
CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA ATGTTGAGAG AATTTGAAGA GACAGAACGC 
AAGGGAGGCT TGACAGGTGG AGATGGCGGG GTTACAAAGT ATGGGTCCTT CGAGGAAGCA 
GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC CGGGAGGGAG ACAACCATAA AGAGGCCATC 
TATGCCCTTC CGAGGAAAGG AAGCCTGGAG CTCGCCCATC CTCCCATCGC CCAGCTTGGT 
GATGACCTGA AGGAATTTTC CACCCCAAAG AGCACCATGC AGGCAAGCCC AGACTGGAAG 
GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC TTGGAGAATG TCACAAAGAG GAAGTTTTCG 
CTGAAAGCCG CGGAGTTCGG GGAACCCACT TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA 
CCGGCTGCTC CGACTGCAAC CCCGGTGTCC TGGAAGCCGC AGGATCCCTC CGAACAGCCT 
CAGGAGAAGC GGTATCAGAG CCCGTGTGCG ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG 
GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC AAAACCATGT CCATTAAAGA AAGATTAGCA 
CTGTTGAAGA AGAGCGGTGA GGAAGACTGG AGAAACAGAC TCAACAGAAA GCAGGAATAC 
GGCAAAGCCT CCATCACCAG CAGCCTGCAC ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG 
AAGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATTG AAGAGAGAAA GCATCTCATC 
ACCGTGAGAG AGGACGCCTG GAAGACCAGG GGCAAAGGAG CAGCCAACGA CTCTACCCAG 
TTTACTGTGG CCGGCAGGAT GGTGAAGCGA GGTCTGGCGT CCCCCACTGC CATCACCCCG 
GTAGCATCCC CTGTTTCCAG CAAAGCAAGG GGCACTACAC CAGTTTCCAG ACCCCTGGAA 
GATATCGAAG CCAGACCAGA CATGCAGTTA GAGTCGGACC TCAAGCTGGA CAGGCTGGAA 
ACCTTCCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AAGAAACAGT CCTCACTGTC 
ACTGGGAAAT CGGTGAAAGA GGTGATGAAG CCGGACGATG ATGAAACCTT TGCCAAGTTT 
TACCGCAGCG TGGACTCCTC TCTACCGAGA AGCCCCGTAG AGCTGGACGA GGATTTCGAT 
GTCATTTTTG ATCCTTATGC CCCCAGGTTG ACATCCTCGG TGGCTGAGCA CAAGCGTGCC 
GTGCGGCCCA AGCGCCGGGT CCAGGCTTCC AAAAACCCCC TGAAGATGCT GGCCGCGAGG 
GAAGATCTCC TGCAGGAGTA CACTGAGCAG AGACTGAACG TGGCCTTCGT GGAGTCAAAG 
CGGATGAAAG TCGAAAAGTT GTCCGCCAAC TCCAGCTTCT CAGAAGTCAC CCTGGCGGGG 
TTAGCCAGCA AAGAAAACTT CAGCAACGTC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ACAGTGCAGT GCCCTACAAG AAGCTGATGC TGCTGCAGGT TAAAGGAAGA 
AGACACGTGC AGACGCGGCT GGTGGAGCCT CGCGCCCCCT CCCTCAACAG CGGGGACTGC 
TTCCTCCTGC TCTCGCCCCA TCACTGCTTC CTGTGGGTCG GAGAGTTCGC CAACGTGATC 
GAGAAGGCGA AGGCCTCAGA ACTTGCGAGT TTAATTCAGA CCAAGAGGGA ACTTGGTTGT 
AGAGCAACTT ACATCCAGAC TGTTGAAGAA GGAATTAATA CACACACACA TGCAGCCAAA 
GACTTCTGGA AGCTTCTGGG CGGCCAAGCC AGTTACCAAT CCGCCGGAGA CCCAAAGGAG 
GATGAGCTCT ATGAAACGGC CATAATAGAG ACCAACTGCA TTTACCGTCT GATGGATGAC 
AAACTCGTTC CTGATGATGA CTACTGGGGG AAGATCCCAA AGTGCTCCCT CCTGCAATCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGGAGT GAAGTTTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCAC AACGGAAAAT CGCCTTTCAG CTGGCAAAGC ACTTGTGGAA TGGAACCTTT 
GACTACGAGA ATTGTGACAT TAACCCGCTG GATCCTGGGG AATGCAATCC CCTCATTCCC 
AGGAAAGGAC AGGGGCGGCC CGACTGGGCA ATATTTGGGA GACTTACAGA ACACAATGAG 
ACGATCTTGT TCAAAGAGAA ATTCCTTGAT TGGACGGAAC TGAAGAGACC TAACGAGAAG 
AACGCCAGCG AACTCGCGCA GCACAAGGAC GATGCCCGGG CAGAGGTCAA GCCTTACGAC 
GTGACGCGGA TGGTGCCCGT GCCCCAGACA ACAGCCGGCA CCGTGCTGGA TGGGGTCAAC 
GTGGGCCGAG GCTACGGGCT GGTGGAAGGG GACGACCGGA GGCAGTTCGA GATCGCCAGC 
ATCTCAGTGG ACGTCTGGCA CATCCTGGAG TTCGACTACA GCAGGCTCCC CAAGCAGAGC 
ATCGGGCAGT TCCACGAAGG CGATGCCTAC GTGGTGAAGT GGAAGTTCAT CGTGAGCACT 
GCAGTGGGAA GCCGGCAGAA GGGGGAGCAC TCGGTCAGGG TGGCTGGCAA AGAGAAATGT 
GTCTACTTCT TCTGGCAAGG CCGCCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGACGA GGAAAGGGGG GCCCAGGTCC AGGTCCTGCA GGGCAAGGAG 
CCCCCCTGTT TCCTGCAGTG CTTCCAGGGG GGGATGGTGG TCCACTCCGG GAGACGGGAG 
GAGGAGGAAG AGAACACACA GAGCGAGTGG CGGCTGTACT GTGTGCGCGG AGAGGTGCCT 
GTGGAGGGGA ACTTGCTGGA GGTGGCCTGT CACTGCAGCA GCCTCAGGTC TAGGACGTCC 
ATGGTTGTCC TCAACGTCCA CAAAGCCCTC ATCTACCTGT GGCATGGATG CAAAGCCCAG 
GCCCACACGA AGGAGGTCGG ACGGACTGCA GCCAATAAAA TCAAGGACCA ATGTCCCCTG 
GAGGCCGGGC TGCACAGTAG CAGCAAAGTG ACAATTCATG AGTGTGATGA AGGCTCGGAG 
CCCCTGGGGT TCTGGGACGC TCTGGGGAGG AGAGACCGGA AAGCCTACGA CTGCATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCC CGCCTGTTCA TCCTCAGCAG CTCCTCTGGT 
GACTTCTCAG CCACAGAGTT CATGTATCCC GCCCGAGACC CCTCTGTGGT CAACTCTATG 
CCCTTCCTGC AGGAAGACCT ATACAGCGCC CCACAGCCAG CACTCTTCCT TGTTGACAAT 
CACCACGAGG TGTACCTCTG GCAAGGCTGG TGGCCCATCG AGAACAAGAT CACGGGCTCC 
GCGCGCATCC GCTGGGCCTC GGACCGGAAG AGCGCCATGG AGACAGTGCT GCAGTACTGC 
CGAGGGAAAA ACCTGAAGAA GCCGCCCCCC AAGTCTTACC TCATCCACGC TGGCCTGGAA 
CCCCTGACCT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAAGACAT TGCAGAGATC 
ACGGAGATGG ACACGGAAGT TTCAAATCAG ATCACCCTAG TGGAGGATGT CTTAGCCAAA 
CTGTGTAAGA CCATTTACCC ACTGGCCGAC CTCCTAGCCA GGCCGCTCCC TGAGGGAGTT 
GACCCTCTGA AGCTGGAGAT CTATCTCACC GACGAGGACT TCGAGTTTGC ACTAGACATG 
ACCCGAGACG AGTACAATGC ACTGCCCGCT TGGAAGCAGG TGAACCTGAA GAAAGCGAAA 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000269.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856037.1 Bos taurus supervillin (SVIL), transcript variant X7, mRNA.

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CloneID	OBa221410
Clone ID Related Accession (Same CDS sequence)	XM_025000269.1
Accession Version	XM_025000269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTGTCAAC TTCAAATTCA GAAATGCCAG CTGCCGAGGA TGAAGAAAAG 
GTCGATGAGC GAGCGAGGCT GAGCGTCGCT GCTAAGAGGC TGCTCTTCAG GGAGATGGAG 
AAGTCGTTTG ACGAGAAGAG CGTCCCAAAG CGCCGCTCCC GCAATGCGGC CGTGGAGCAG 
AGGCTGCGGC GCCTCCAGGA CCGGTCCCAC ACGCAGCCCG TCACCACCGA GGAGGTGGTC 
ATTGCAGCCA CTGAACCTAC CCCCGCTTCG TGCTCAGTGG CCACCCACCC TGTAATGACA 
AGACATCCTA GCCCCACTGT AGCTAAGAGT CCCGTGCAGC CTGCCAGATT GCAGGCGTCT 
GCTCACCAAA AGGCACTAGC CAGAGACCAG ACAAACGAGA GCAAAGATTC TGCTGAGCAG 
GGAGAACCTG ACTCCTCCAC TCTGAGCTTA GCGGAGAAGC TGGCCTTGTT TAACAAACTA 
TCTCAGCCGG TCTCAAAAGC CATTTCAACA CGGAACAGAT TAGACATGAG ACAGAGGAGG 
ATGAATGCTC GTTATCAAAC CCAGCCGGTC ACGCTTGGAG AAGTGGAACA GGTGCAGAGT 
GGCAAGCTCA TGGCTTTCTC TCCCACCATT AACACTTCCG TGTCCACCGT GGCATCTACC 
GTCCCCCCCA TGTATGCAGG AAATCTTCGG ACAAAGCCAC TTCCAGATGA CAGCTTTGGT 
GCCACTGAGC AGAAGTTTGC TTCTTCGCTA GAAAACTCCG ACTCCCCAGT TAGAAGCATC 
CTGAAATCCC AAGGCTGGCA GCCCTCGGTC GAGGGTGCTG GGAGCAAAGC AATGTTGAGA 
GAATTTGAAG AGACAGAACG CAAGGGAGGC TTGACAGGTG GAGATGGCGG GGTTACAAAG 
TATGGGTCCT TCGAGGAAGC AGAGCTGTCC TACCCCGTCC TCAGCAGAGT CCGGGAGGGA 
GACAACCATA AAGAGGCCAT CTATGCCCTT CCGAGGAAAG GAAGCCTGGA GCTCGCCCAT 
CCTCCCATCG CCCAGCTTGG TGATGACCTG AAGGAATTTT CCACCCCAAA GAGCACCATG 
CAGGCAAGCC CAGACTGGAA GGAGAGGCAG CTCTTTGAAG AGAAGGTGGA CTTGGAGAAT 
GTCACAAAGA GGAAGTTTTC GCTGAAAGCC GCGGAGTTCG GGGAACCCAC TTCTGAGCAG 
ACCGGCGCGG CTGCTGGGAA ACCGGCTGCT CCGACTGCAA CCCCGGTGTC CTGGAAGCCG 
CAGGATCCCT CCGAACAGCC TCAGGAGAAG CGGTATCAGA GCCCGTGTGC GATGTTTGCT 
GCTGGAGAGA TCAAAGCCCC GGCGGTGGAG GGCAGCCTCG ACTCCCCCAG CAAAACCATG 
TCCATTAAAG AAAGATTAGC ACTGTTGAAG AAGAGCGGTG AGGAAGACTG GAGAAACAGA 
CTCAACAGAA AGCAGGAATA CGGCAAAGCC TCCATCACCA GCAGCCTGCA CATCCAAGAG 
ACGGAGCAGT CACTCAAGAA GAAGGAAGAA GGAGGATTTA CAGATGATAA TGACATTTCT 
AATCTGCTTT GGGAACCTGT ATACGCTTCT ACTTATTCTC CTGCTCTACC TGCTGCCCAT 
AAATACCTGT CTTTTGTATC TATTAATCAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGCGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856037.1
CDS	356..7174
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000269.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000269.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTGTCAAC TTCAAATTCA GAAATGCCAG CTGCCGAGGA TGAAGAAAAG 
GTCGATGAGC GAGCGAGGCT GAGCGTCGCT GCTAAGAGGC TGCTCTTCAG GGAGATGGAG 
AAGTCGTTTG ACGAGAAGAG CGTCCCAAAG CGCCGCTCCC GCAATGCGGC CGTGGAGCAG 
AGGCTGCGGC GCCTCCAGGA CCGGTCCCAC ACGCAGCCCG TCACCACCGA GGAGGTGGTC 
ATTGCAGCCA CTGAACCTAC CCCCGCTTCG TGCTCAGTGG CCACCCACCC TGTAATGACA 
AGACATCCTA GCCCCACTGT AGCTAAGAGT CCCGTGCAGC CTGCCAGATT GCAGGCGTCT 
GCTCACCAAA AGGCACTAGC CAGAGACCAG ACAAACGAGA GCAAAGATTC TGCTGAGCAG 
GGAGAACCTG ACTCCTCCAC TCTGAGCTTA GCGGAGAAGC TGGCCTTGTT TAACAAACTA 
TCTCAGCCGG TCTCAAAAGC CATTTCAACA CGGAACAGAT TAGACATGAG ACAGAGGAGG 
ATGAATGCTC GTTATCAAAC CCAGCCGGTC ACGCTTGGAG AAGTGGAACA GGTGCAGAGT 
GGCAAGCTCA TGGCTTTCTC TCCCACCATT AACACTTCCG TGTCCACCGT GGCATCTACC 
GTCCCCCCCA TGTATGCAGG AAATCTTCGG ACAAAGCCAC TTCCAGATGA CAGCTTTGGT 
GCCACTGAGC AGAAGTTTGC TTCTTCGCTA GAAAACTCCG ACTCCCCAGT TAGAAGCATC 
CTGAAATCCC AAGGCTGGCA GCCCTCGGTC GAGGGTGCTG GGAGCAAAGC AATGTTGAGA 
GAATTTGAAG AGACAGAACG CAAGGGAGGC TTGACAGGTG GAGATGGCGG GGTTACAAAG 
TATGGGTCCT TCGAGGAAGC AGAGCTGTCC TACCCCGTCC TCAGCAGAGT CCGGGAGGGA 
GACAACCATA AAGAGGCCAT CTATGCCCTT CCGAGGAAAG GAAGCCTGGA GCTCGCCCAT 
CCTCCCATCG CCCAGCTTGG TGATGACCTG AAGGAATTTT CCACCCCAAA GAGCACCATG 
CAGGCAAGCC CAGACTGGAA GGAGAGGCAG CTCTTTGAAG AGAAGGTGGA CTTGGAGAAT 
GTCACAAAGA GGAAGTTTTC GCTGAAAGCC GCGGAGTTCG GGGAACCCAC TTCTGAGCAG 
ACCGGCGCGG CTGCTGGGAA ACCGGCTGCT CCGACTGCAA CCCCGGTGTC CTGGAAGCCG 
CAGGATCCCT CCGAACAGCC TCAGGAGAAG CGGTATCAGA GCCCGTGTGC GATGTTTGCT 
GCTGGAGAGA TCAAAGCCCC GGCGGTGGAG GGCAGCCTCG ACTCCCCCAG CAAAACCATG 
TCCATTAAAG AAAGATTAGC ACTGTTGAAG AAGAGCGGTG AGGAAGACTG GAGAAACAGA 
CTCAACAGAA AGCAGGAATA CGGCAAAGCC TCCATCACCA GCAGCCTGCA CATCCAAGAG 
ACGGAGCAGT CACTCAAGAA GAAGGAAGAA GGAGGATTTA CAGATGATAA TGACATTTCT 
AATCTGCTTT GGGAACCTGT ATACGCTTCT ACTTATTCTC CTGCTCTACC TGCTGCCCAT 
AAATACCTGT CTTTTGTATC TATTAATCAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGCGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000270.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856038.1 Bos taurus supervillin (SVIL), transcript variant X8, mRNA.

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CloneID	OBa221411
Clone ID Related Accession (Same CDS sequence)	XM_025000270.1
Accession Version	XM_025000270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGAGT TAAAGTTAGA 
GAAAAATTGG TGCGAGAGGA GAGCGCTCGC AGCAGCCCTG AACTTACCTC CGAGTCCTTA 
ACTCAGAGGA GACACCAGAC GGCACCAGGC CATTACCTGG CCTTTCAGTC AGAGAACTCG 
GCCTTCGATA GGGTCTCGGG CAAGGTGGCC AGCTCCGCAA GACAGCCCAT CCGCGGCTAC 
GTCCAGCCAG CAGAGCCTGT CCACACCATC ACGCTGGTGA CCTCAGACAC CCCAGAAAGC 
ATCTCCGAGG GCAGCTGGGT GGGTCCAGCC CCCCAGACTG TCACAAAGCC TCCCCCCTCA 
AAGGTTCTAG AAGGTGAAAG AAGAGACACC CCGGTTCTCC ATATTTGCGA ATCCAAAGCA 
GAAGATGTGC TCTTCTCTGA TGCTCTGGAG AAAACCAGGA AGACCCTTGC AGTTTTGGAG 
GATCGTGGGT CTGGGAGAAG CCAGGAAGCC CCCTCTGGAA CTGAGGACTT GAGTCAGCCC 
GCTGTTGGCA TAGTTACCGC GGAACCTCAG AAGGAATCAG AAAGCCTGGC CCACCCGCCT 
ATGGCTCAGC AGCAGCCCAC TGAGAGGATG GGCAGGAGTG AGATGGTCAT GTATGTTCAG 
AGCGAGGCTG TGTCCCAAGG TCACAGGAAG GAGGTGCCCA CGAGGAAACA CAGGGTCCTC 
ACCCGCTCCC TGTCCGACTA CACGGGCCCT CCACAGCTCC AGGCTCTGAA GGCCAAGGCC 
CCGGCTCCCA AGCGAGATGC CGAGTCACAG ACGTCCAAGG CCGAGTTGGA ACTGGGCCTG 
CTGGACACGA AGGTCTCGGT CGCCCAGCTC CGCAATGCGT TCCTGGAGTC GGCCAGGGCC 
AGCAGGAAAC CCGAGCTCCA TTCTCGGGTT GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG 
CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC TATTTTTCTC CTGGTGAAAA TAGAAAGACT 
TCCGAGAGAT TTAGAACTCA ACCTATAACC TCGGCAGAAC GAAAGGAATC GGATAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACTGAACCT 
ACCCCCGCTT CGTGCTCAGT GGCCACCCAC CCTGTAATGA CAAGACATCC TAGCCCCACT 
GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA TTGCAGGCGT CTGCTCACCA AAAGGCACTA 
GCCAGAGACC AGACAAACGA GAGCAAAGAT TCTGCTGAGC AGGGAGAACC TGACTCCTCC 
ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG TTTAACAAAC TATCTCAGCC GGTCTCAAAA 
GCCATTTCAA CACGGAACAG ATTAGACATG AGACAGAGGA GGATGAATGC TCGTTATCAA 
ACCCAGCCGG TCACGCTTGG AGAAGTGGAA CAGGTGCAGA GTGGCAAGCT CATGGCTTTC 
TCTCCCACCA TTAACACTTC CGTGTCCACC GTGGCATCTA CCGTCCCCCC CATGTATGCA 
GGAAATCTTC GGACAAAGCC ACTTCCAGAT GACAGCTTTG GTGCCACTGA GCAGAAGTTT 
GCTTCTTCGC TAGAAAACTC CGACTCCCCA GTTAGAAGCA TCCTGAAATC CCAAGGCTGG 
CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA GCAATGTTGA GAGAATTTGA AGAGACAGAA 
CGCAAGGGAG GCTTGACAGG TGGAGATGGC GGGGTTACAA AGTATGGGTC CTTCGAGGAA 
GCAGAGCTGT CCTACCCCGT CCTCAGCAGA GTCCGGGAGG GAGACAACCA TAAAGAGGCC 
ATCTATGCCC TTCCGAGGAA AGGAAGCCTG GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT 
GGTGATGACC TGAAGGAATT TTCCACCCCA AAGAGCACCA TGCAGGCAAG CCCAGACTGG 
AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG GACTTGGAGA ATGTCACAAA GAGGAAGTTT 
TCGCTGAAAG CCGCGGAGTT CGGGGAACCC ACTTCTGAGC AGACCGGCGC GGCTGCTGGG 
AAACCGGCTG CTCCGACTGC AACCCCGGTG TCCTGGAAGC CGCAGGATCC CTCCGAACAG 
CCTCAGGAGA AGCGGTATCA GAGCCCGTGT GCGATGTTTG CTGCTGGAGA GATCAAAGCC 
CCGGCGGTGG AGGGCAGCCT CGACTCCCCC AGCAAAACCA TGTCCATTAA AGAAAGATTA 
GCACTGTTGA AGAAGAGCGG TGAGGAAGAC TGGAGAAACA GACTCAACAG AAAGCAGGAA 
TACGGCAAAG CCTCCATCAC CAGCAGCCTG CACATCCAAG AGACGGAGCA GTCACTCAAG 
AAGAAGCGGG TCACAGAAAG TCGAGAGAGC CAGATGACTA TTGAAGAGAG AAAGCATCTC 
ATCACCGTGA GAGAGGACGC CTGGAAGACC AGGGGCAAAG GAGCAGCCAA CGACTCTACC 
CAGTTTACTG TGGCCGGCAG GATGGTGAAG CGAGGTCTGG CGTCCCCCAC TGCCATCACC 
CCGGTAGCAT CCCCTGTTTC CAGCAAAGCA AGGGGCACTA CACCAGTTTC CAGACCCCTG 
GAAGATATCG AAGCCAGACC AGACATGCAG TTAGAGTCGG ACCTCAAGCT GGACAGGCTG 
GAAACCTTCC TAAGAAGGCT GAATAACAAA GTTGGTGGGA TGCAAGAAAC AGTCCTCACT 
GTCACTGGGA AATCGGTGAA AGAGGTGATG AAGCCGGACG ATGATGAAAC CTTTGCCAAG 
TTTTACCGCA GCGTGGACTC CTCTCTACCG AGAAGCCCCG TAGAGCTGGA CGAGGATTTC 
GATGTCATTT TTGATCCTTA TGCCCCCAGG TTGACATCCT CGGTGGCTGA GCACAAGCGT 
GCCGTGCGGC CCAAGCGCCG GGTCCAGGCT TCCAAAAACC CCCTGAAGAT GCTGGCCGCG 
AGGGAAGATC TCCTGCAGGA GTACACTGAG CAGAGACTGA ACGTGGCCTT CGTGGAGTCA 
AAGCGGATGA AAGTCGAAAA GTTGTCCGCC AACTCCAGCT TCTCAGAAGT CACCCTGGCG 
GGGTTAGCCA GCAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAATTCCA ACAACAGTGC AGTGCCCTAC AAGAAGCTGA TGCTGCTGCA GGTTAAAGGA 
AGAAGACACG TGCAGACGCG GCTGGTGGAG CCTCGCGCCC CCTCCCTCAA CAGCGGGGAC 
TGCTTCCTCC TGCTCTCGCC CCATCACTGC TTCCTGTGGG TCGGAGAGTT CGCCAACGTG 
ATCGAGAAGG CGAAGGCCTC AGAACTTGCG AGTTTAATTC AGACCAAGAG GGAACTTGGT 
TGTAGAGCAA CTTACATCCA GACTGTTGAA GAAGGAATTA ATACACACAC ACATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGCGGCCAA GCCAGTTACC AATCCGCCGG AGACCCAAAG 
GAGGATGAGC TCTATGAAAC GGCCATAATA GAGACCAACT GCATTTACCG TCTGATGGAT 
GACAAACTCG TTCCTGATGA TGACTACTGG GGGAAGATCC CAAAGTGCTC CCTCCTGCAA 
TCAAAAGAGG TACTGGTGTT TGATTTTGGG AGTGAAGTTT ACGTGTGGCA TGGAAAAGAA 
GTCACATTAG CACAACGGAA AATCGCCTTT CAGCTGGCAA AGCACTTGTG GAATGGAACC 
TTTGACTACG AGAATTGTGA CATTAACCCG CTGGATCCTG GGGAATGCAA TCCCCTCATT 
CCCAGGAAAG GACAGGGGCG GCCCGACTGG GCAATATTTG GGAGACTTAC AGAACACAAT 
GAGACGATCT TGTTCAAAGA GAAATTCCTT GATTGGACGG AACTGAAGAG ACCTAACGAG 
AAGAACGCCA GCGAACTCGC GCAGCACAAG GACGATGCCC GGGCAGAGGT CAAGCCTTAC 
GACGTGACGC GGATGGTGCC CGTGCCCCAG ACAACAGCCG GCACCGTGCT GGATGGGGTC 
AACGTGGGCC GAGGCTACGG GCTGGTGGAA GGGGACGACC GGAGGCAGTT CGAGATCGCC 
AGCATCTCAG TGGACGTCTG GCACATCCTG GAGTTCGACT ACAGCAGGCT CCCCAAGCAG 
AGCATCGGGC AGTTCCACGA AGGCGATGCC TACGTGGTGA AGTGGAAGTT CATCGTGAGC 
ACTGCAGTGG GAAGCCGGCA GAAGGGGGAG CACTCGGTCA GGGTGGCTGG CAAAGAGAAA 
TGTGTCTACT TCTTCTGGCA AGGCCGCCAG TCGACCGTGA GTGAGAAGGG CACATCAGCT 
CTGATGACAG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTCCT GCAGGGCAAG 
GAGCCCCCCT GTTTCCTGCA GTGCTTCCAG GGGGGGATGG TGGTCCACTC CGGGAGACGG 
GAGGAGGAGG AAGAGAACAC ACAGAGCGAG TGGCGGCTGT ACTGTGTGCG CGGAGAGGTG 
CCTGTGGAGG GGAACTTGCT GGAGGTGGCC TGTCACTGCA GCAGCCTCAG GTCTAGGACG 
TCCATGGTTG TCCTCAACGT CCACAAAGCC CTCATCTACC TGTGGCATGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGACGGACT GCAGCCAATA AAATCAAGGA CCAATGTCCC 
CTGGAGGCCG GGCTGCACAG TAGCAGCAAA GTGACAATTC ATGAGTGTGA TGAAGGCTCG 
GAGCCCCTGG GGTTCTGGGA CGCTCTGGGG AGGAGAGACC GGAAAGCCTA CGACTGCATG 
CTTCAAGATC CTGGAAATTT TAACTTCACA CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGTGACTTCT CAGCCACAGA GTTCATGTAT CCCGCCCGAG ACCCCTCTGT GGTCAACTCT 
ATGCCCTTCC TGCAGGAAGA CCTATACAGC GCCCCACAGC CAGCACTCTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACGGGC 
TCCGCGCGCA TCCGCTGGGC CTCGGACCGG AAGAGCGCCA TGGAGACAGT GCTGCAGTAC 
TGCCGAGGGA AAAACCTGAA GAAGCCGCCC CCCAAGTCTT ACCTCATCCA CGCTGGCCTG 
GAACCCCTGA CCTTCACCAA CATGTTCCCC AGCTGGGAGC ACAGAGAAGA CATTGCAGAG 
ATCACGGAGA TGGACACGGA AGTTTCAAAT CAGATCACCC TAGTGGAGGA TGTCTTAGCC 
AAACTGTGTA AGACCATTTA CCCACTGGCC GACCTCCTAG CCAGGCCGCT CCCTGAGGGA 
GTTGACCCTC TGAAGCTGGA GATCTATCTC ACCGACGAGG ACTTCGAGTT TGCACTAGAC 
ATGACCCGAG ACGAGTACAA TGCACTGCCC GCTTGGAAGC AGGTGAACCT GAAGAAAGCG 
AAAGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856038.1
CDS	356..7150
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000270.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000270.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGAGT TAAAGTTAGA 
GAAAAATTGG TGCGAGAGGA GAGCGCTCGC AGCAGCCCTG AACTTACCTC CGAGTCCTTA 
ACTCAGAGGA GACACCAGAC GGCACCAGGC CATTACCTGG CCTTTCAGTC AGAGAACTCG 
GCCTTCGATA GGGTCTCGGG CAAGGTGGCC AGCTCCGCAA GACAGCCCAT CCGCGGCTAC 
GTCCAGCCAG CAGAGCCTGT CCACACCATC ACGCTGGTGA CCTCAGACAC CCCAGAAAGC 
ATCTCCGAGG GCAGCTGGGT GGGTCCAGCC CCCCAGACTG TCACAAAGCC TCCCCCCTCA 
AAGGTTCTAG AAGGTGAAAG AAGAGACACC CCGGTTCTCC ATATTTGCGA ATCCAAAGCA 
GAAGATGTGC TCTTCTCTGA TGCTCTGGAG AAAACCAGGA AGACCCTTGC AGTTTTGGAG 
GATCGTGGGT CTGGGAGAAG CCAGGAAGCC CCCTCTGGAA CTGAGGACTT GAGTCAGCCC 
GCTGTTGGCA TAGTTACCGC GGAACCTCAG AAGGAATCAG AAAGCCTGGC CCACCCGCCT 
ATGGCTCAGC AGCAGCCCAC TGAGAGGATG GGCAGGAGTG AGATGGTCAT GTATGTTCAG 
AGCGAGGCTG TGTCCCAAGG TCACAGGAAG GAGGTGCCCA CGAGGAAACA CAGGGTCCTC 
ACCCGCTCCC TGTCCGACTA CACGGGCCCT CCACAGCTCC AGGCTCTGAA GGCCAAGGCC 
CCGGCTCCCA AGCGAGATGC CGAGTCACAG ACGTCCAAGG CCGAGTTGGA ACTGGGCCTG 
CTGGACACGA AGGTCTCGGT CGCCCAGCTC CGCAATGCGT TCCTGGAGTC GGCCAGGGCC 
AGCAGGAAAC CCGAGCTCCA TTCTCGGGTT GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG 
CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC TATTTTTCTC CTGGTGAAAA TAGAAAGACT 
TCCGAGAGAT TTAGAACTCA ACCTATAACC TCGGCAGAAC GAAAGGAATC GGATAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACTGAACCT 
ACCCCCGCTT CGTGCTCAGT GGCCACCCAC CCTGTAATGA CAAGACATCC TAGCCCCACT 
GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA TTGCAGGCGT CTGCTCACCA AAAGGCACTA 
GCCAGAGACC AGACAAACGA GAGCAAAGAT TCTGCTGAGC AGGGAGAACC TGACTCCTCC 
ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG TTTAACAAAC TATCTCAGCC GGTCTCAAAA 
GCCATTTCAA CACGGAACAG ATTAGACATG AGACAGAGGA GGATGAATGC TCGTTATCAA 
ACCCAGCCGG TCACGCTTGG AGAAGTGGAA CAGGTGCAGA GTGGCAAGCT CATGGCTTTC 
TCTCCCACCA TTAACACTTC CGTGTCCACC GTGGCATCTA CCGTCCCCCC CATGTATGCA 
GGAAATCTTC GGACAAAGCC ACTTCCAGAT GACAGCTTTG GTGCCACTGA GCAGAAGTTT 
GCTTCTTCGC TAGAAAACTC CGACTCCCCA GTTAGAAGCA TCCTGAAATC CCAAGGCTGG 
CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA GCAATGTTGA GAGAATTTGA AGAGACAGAA 
CGCAAGGGAG GCTTGACAGG TGGAGATGGC GGGGTTACAA AGTATGGGTC CTTCGAGGAA 
GCAGAGCTGT CCTACCCCGT CCTCAGCAGA GTCCGGGAGG GAGACAACCA TAAAGAGGCC 
ATCTATGCCC TTCCGAGGAA AGGAAGCCTG GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT 
GGTGATGACC TGAAGGAATT TTCCACCCCA AAGAGCACCA TGCAGGCAAG CCCAGACTGG 
AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG GACTTGGAGA ATGTCACAAA GAGGAAGTTT 
TCGCTGAAAG CCGCGGAGTT CGGGGAACCC ACTTCTGAGC AGACCGGCGC GGCTGCTGGG 
AAACCGGCTG CTCCGACTGC AACCCCGGTG TCCTGGAAGC CGCAGGATCC CTCCGAACAG 
CCTCAGGAGA AGCGGTATCA GAGCCCGTGT GCGATGTTTG CTGCTGGAGA GATCAAAGCC 
CCGGCGGTGG AGGGCAGCCT CGACTCCCCC AGCAAAACCA TGTCCATTAA AGAAAGATTA 
GCACTGTTGA AGAAGAGCGG TGAGGAAGAC TGGAGAAACA GACTCAACAG AAAGCAGGAA 
TACGGCAAAG CCTCCATCAC CAGCAGCCTG CACATCCAAG AGACGGAGCA GTCACTCAAG 
AAGAAGCGGG TCACAGAAAG TCGAGAGAGC CAGATGACTA TTGAAGAGAG AAAGCATCTC 
ATCACCGTGA GAGAGGACGC CTGGAAGACC AGGGGCAAAG GAGCAGCCAA CGACTCTACC 
CAGTTTACTG TGGCCGGCAG GATGGTGAAG CGAGGTCTGG CGTCCCCCAC TGCCATCACC 
CCGGTAGCAT CCCCTGTTTC CAGCAAAGCA AGGGGCACTA CACCAGTTTC CAGACCCCTG 
GAAGATATCG AAGCCAGACC AGACATGCAG TTAGAGTCGG ACCTCAAGCT GGACAGGCTG 
GAAACCTTCC TAAGAAGGCT GAATAACAAA GTTGGTGGGA TGCAAGAAAC AGTCCTCACT 
GTCACTGGGA AATCGGTGAA AGAGGTGATG AAGCCGGACG ATGATGAAAC CTTTGCCAAG 
TTTTACCGCA GCGTGGACTC CTCTCTACCG AGAAGCCCCG TAGAGCTGGA CGAGGATTTC 
GATGTCATTT TTGATCCTTA TGCCCCCAGG TTGACATCCT CGGTGGCTGA GCACAAGCGT 
GCCGTGCGGC CCAAGCGCCG GGTCCAGGCT TCCAAAAACC CCCTGAAGAT GCTGGCCGCG 
AGGGAAGATC TCCTGCAGGA GTACACTGAG CAGAGACTGA ACGTGGCCTT CGTGGAGTCA 
AAGCGGATGA AAGTCGAAAA GTTGTCCGCC AACTCCAGCT TCTCAGAAGT CACCCTGGCG 
GGGTTAGCCA GCAAAGAAAA CTTCAGCAAC GTCAGCCTGC GGAGCGTCAA CCTGACGGAA 
CAGAATTCCA ACAACAGTGC AGTGCCCTAC AAGAAGCTGA TGCTGCTGCA GGTTAAAGGA 
AGAAGACACG TGCAGACGCG GCTGGTGGAG CCTCGCGCCC CCTCCCTCAA CAGCGGGGAC 
TGCTTCCTCC TGCTCTCGCC CCATCACTGC TTCCTGTGGG TCGGAGAGTT CGCCAACGTG 
ATCGAGAAGG CGAAGGCCTC AGAACTTGCG AGTTTAATTC AGACCAAGAG GGAACTTGGT 
TGTAGAGCAA CTTACATCCA GACTGTTGAA GAAGGAATTA ATACACACAC ACATGCAGCC 
AAAGACTTCT GGAAGCTTCT GGGCGGCCAA GCCAGTTACC AATCCGCCGG AGACCCAAAG 
GAGGATGAGC TCTATGAAAC GGCCATAATA GAGACCAACT GCATTTACCG TCTGATGGAT 
GACAAACTCG TTCCTGATGA TGACTACTGG GGGAAGATCC CAAAGTGCTC CCTCCTGCAA 
TCAAAAGAGG TACTGGTGTT TGATTTTGGG AGTGAAGTTT ACGTGTGGCA TGGAAAAGAA 
GTCACATTAG CACAACGGAA AATCGCCTTT CAGCTGGCAA AGCACTTGTG GAATGGAACC 
TTTGACTACG AGAATTGTGA CATTAACCCG CTGGATCCTG GGGAATGCAA TCCCCTCATT 
CCCAGGAAAG GACAGGGGCG GCCCGACTGG GCAATATTTG GGAGACTTAC AGAACACAAT 
GAGACGATCT TGTTCAAAGA GAAATTCCTT GATTGGACGG AACTGAAGAG ACCTAACGAG 
AAGAACGCCA GCGAACTCGC GCAGCACAAG GACGATGCCC GGGCAGAGGT CAAGCCTTAC 
GACGTGACGC GGATGGTGCC CGTGCCCCAG ACAACAGCCG GCACCGTGCT GGATGGGGTC 
AACGTGGGCC GAGGCTACGG GCTGGTGGAA GGGGACGACC GGAGGCAGTT CGAGATCGCC 
AGCATCTCAG TGGACGTCTG GCACATCCTG GAGTTCGACT ACAGCAGGCT CCCCAAGCAG 
AGCATCGGGC AGTTCCACGA AGGCGATGCC TACGTGGTGA AGTGGAAGTT CATCGTGAGC 
ACTGCAGTGG GAAGCCGGCA GAAGGGGGAG CACTCGGTCA GGGTGGCTGG CAAAGAGAAA 
TGTGTCTACT TCTTCTGGCA AGGCCGCCAG TCGACCGTGA GTGAGAAGGG CACATCAGCT 
CTGATGACAG TGGAGCTGGA CGAGGAAAGG GGGGCCCAGG TCCAGGTCCT GCAGGGCAAG 
GAGCCCCCCT GTTTCCTGCA GTGCTTCCAG GGGGGGATGG TGGTCCACTC CGGGAGACGG 
GAGGAGGAGG AAGAGAACAC ACAGAGCGAG TGGCGGCTGT ACTGTGTGCG CGGAGAGGTG 
CCTGTGGAGG GGAACTTGCT GGAGGTGGCC TGTCACTGCA GCAGCCTCAG GTCTAGGACG 
TCCATGGTTG TCCTCAACGT CCACAAAGCC CTCATCTACC TGTGGCATGG ATGCAAAGCC 
CAGGCCCACA CGAAGGAGGT CGGACGGACT GCAGCCAATA AAATCAAGGA CCAATGTCCC 
CTGGAGGCCG GGCTGCACAG TAGCAGCAAA GTGACAATTC ATGAGTGTGA TGAAGGCTCG 
GAGCCCCTGG GGTTCTGGGA CGCTCTGGGG AGGAGAGACC GGAAAGCCTA CGACTGCATG 
CTTCAAGATC CTGGAAATTT TAACTTCACA CCCCGCCTGT TCATCCTCAG CAGCTCCTCT 
GGTGACTTCT CAGCCACAGA GTTCATGTAT CCCGCCCGAG ACCCCTCTGT GGTCAACTCT 
ATGCCCTTCC TGCAGGAAGA CCTATACAGC GCCCCACAGC CAGCACTCTT CCTTGTTGAC 
AATCACCACG AGGTGTACCT CTGGCAAGGC TGGTGGCCCA TCGAGAACAA GATCACGGGC 
TCCGCGCGCA TCCGCTGGGC CTCGGACCGG AAGAGCGCCA TGGAGACAGT GCTGCAGTAC 
TGCCGAGGGA AAAACCTGAA GAAGCCGCCC CCCAAGTCTT ACCTCATCCA CGCTGGCCTG 
GAACCCCTGA CCTTCACCAA CATGTTCCCC AGCTGGGAGC ACAGAGAAGA CATTGCAGAG 
ATCACGGAGA TGGACACGGA AGTTTCAAAT CAGATCACCC TAGTGGAGGA TGTCTTAGCC 
AAACTGTGTA AGACCATTTA CCCACTGGCC GACCTCCTAG CCAGGCCGCT CCCTGAGGGA 
GTTGACCCTC TGAAGCTGGA GATCTATCTC ACCGACGAGG ACTTCGAGTT TGCACTAGAC 
ATGACCCGAG ACGAGTACAA TGCACTGCCC GCTTGGAAGC AGGTGAACCT GAAGAAAGCG 
AAAGGTCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000272.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856040.1 Bos taurus supervillin (SVIL), transcript variant X10, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856040.1
CDS	746..7513
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000272.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000272.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000278.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856046.1 Bos taurus supervillin (SVIL), transcript variant X16, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856046.1
CDS	495..7262
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000278.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000278.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000283.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856051.1 Bos taurus supervillin (SVIL), transcript variant X21, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856051.1
CDS	311..7078
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000283.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000283.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000273.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856041.1 Bos taurus supervillin (SVIL), transcript variant X11, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856041.1
CDS	748..7515
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000273.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000273.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000277.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856045.1 Bos taurus supervillin (SVIL), transcript variant X15, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856045.1
CDS	530..7297
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000277.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000277.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000271.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856039.1 Bos taurus supervillin (SVIL), transcript variant X9, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856039.1
CDS	734..7501
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000271.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000271.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000275.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856043.1 Bos taurus supervillin (SVIL), transcript variant X13, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856043.1
CDS	588..7355
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000275.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000275.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000281.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856049.1 Bos taurus supervillin (SVIL), transcript variant X19, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856049.1
CDS	427..7194
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000281.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000281.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000274.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856042.1 Bos taurus supervillin (SVIL), transcript variant X12, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856042.1
CDS	642..7409
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000274.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000274.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000276.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856044.1 Bos taurus supervillin (SVIL), transcript variant X14, mRNA.

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CloneID	OBa221412
Clone ID Related Accession (Same CDS sequence)	XM_025000272.1 , XM_025000278.1 , XM_025000283.1 , XM_025000273.1 , XM_025000277.1 , XM_025000271.1 , XM_025000275.1 , XM_025000281.1 , XM_025000274.1 , XM_025000276.1
Accession Version	XM_025000276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856044.1
CDS	369..7136
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000276.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000276.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTCTG 
GTATTGAAGG ACTCTCACTG TGTTTCCAGT CTGGTATTGA AGGACTCTCA CAGTATCGGG 
AAACCCAAAC ATGAGTGGTT TCTCCAGAAA GATTCCGAAG GGGACACACC TTCACTTATC 
AACTGGCCAT CCAGAGTTAA AGTTAGAGAA AAATTGGTGC GAGAGGAGAG CGCTCGCAGC 
AGCCCTGAAC TTACCTCCGA GTCCTTAACT CAGAGGAGAC ACCAGACGGC ACCAGGCCAT 
TACCTGGCCT TTCAGTCAGA GAACTCGGCC TTCGATAGGG TCTCGGGCAA GGTGGCCAGC 
TCCGCAAGAC AGCCCATCCG CGGCTACGTC CAGCCAGCAG AGCCTGTCCA CACCATCACG 
CTGGTGACCT CAGACACCCC AGAAAGCATC TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC 
CAGACTGTCA CAAAGCCTCC CCCCTCAAAG GTTCTAGAAG GTGAAAGAAG AGACACCCCG 
GTTCTCCATA TTTGCGAATC CAAAGCAGAA GATGTGCTCT TCTCTGATGC TCTGGAGAAA 
ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC 
TCTGGAACTG AGGACTTGAG TCAGCCCGCT GTTGGCATAG TTACCGCGGA ACCTCAGAAG 
GAATCAGAAA GCCTGGCCCA CCCGCCTATG GCTCAGCAGC AGCCCACTGA GAGGATGGGC 
AGGAGTGAGA TGGTCATGTA TGTTCAGAGC GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG 
GTGCCCACGA GGAAACACAG GGTCCTCACC CGCTCCCTGT CCGACTACAC GGGCCCTCCA 
CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG GCTCCCAAGC GAGATGCCGA GTCACAGACG 
TCCAAGGCCG AGTTGGAACT GGGCCTGCTG GACACGAAGG TCTCGGTCGC CCAGCTCCGC 
AATGCGTTCC TGGAGTCGGC CAGGGCCAGC AGGAAACCCG AGCTCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000279.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856047.1 Bos taurus supervillin (SVIL), transcript variant X17, mRNA.

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CloneID	OBa221413
Clone ID Related Accession (Same CDS sequence)	XM_025000279.1
Accession Version	XM_025000279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGAGT TAAAGTTAGA 
GAAAAATTGG TGCGAGAGGA GAGCGCTCGC AGCAGCCCTG AACTTACCTC CGAGTCCTTA 
ACTCAGAGGA GACACCAGAC GGCACCAGGC CATTACCTGG CCTTTCAGTC AGAGAACTCG 
GCCTTCGATA GGGTCTCGGG CAAGGTGGCC AGCTCCGCAA GACAGCCCAT CCGCGGCTAC 
GTCCAGCCAG CAGAGCCTGT CCACACCATC ACGCTGGTGA CCTCAGACAC CCCAGAAAGC 
ATCTCCGAGG GCAGCTGGGT GGGTCCAGCC CCCCAGACTG TCACAAAGCC TCCCCCCTCA 
AAGGTTCTAG AAGGTGAAAG AAGAGACACC CCGGTTCTCC ATATTTGCGA ATCCAAAGCA 
GAAGATGTGC TCTTCTCTGA TGCTCTGGAG AAAACCAGGA AGACCCTTGC AGTTTTGGAG 
GATCGTGGGT CTGGGAGAAG CCAGGAAGCC CCCTCTGGAA CTGAGGACTT GAGTCAGCCC 
GCTGTTGGCA TAGTTACCGC GGAACCTCAG AAGGAATCAG AAAGCCTGGC CCACCCGCCT 
ATGGCTCAGC AGCAGCCCAC TGAGAGGATG GGCAGGAGTG AGATGGTCAT GTATGTTCAG 
AGCGAGGCTG TGTCCCAAGG TCACAGGAAG GAGGTGCCCA CGAGGAAACA CAGGGTCCTC 
ACCCGCTCCC TGTCCGACTA CACGGGCCCT CCACAGCTCC AGGCTCTGAA GGCCAAGGCC 
CCGGCTCCCA AGCGAGATGC CGAGTCACAG ACGTCCAAGG CCGAGTTGGA ACTGGGCCTG 
CTGGACACGA AGGTCTCGGT CGCCCAGCTC CGCAATGCGT TCCTGGAGTC GGCCAGGGCC 
AGCAGGAAAC CCGAGCTCCA TTCTCGGGTT GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG 
CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC TATTTTTCTC CTGGTGAAAA TAGAAAGACT 
TCCGAGAGAT TTAGAACTCA ACCTATAACC TCGGCAGAAC GAAAGGAATC GGATAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGCGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856047.1
CDS	356..7054
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000279.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000279.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGAGT TAAAGTTAGA 
GAAAAATTGG TGCGAGAGGA GAGCGCTCGC AGCAGCCCTG AACTTACCTC CGAGTCCTTA 
ACTCAGAGGA GACACCAGAC GGCACCAGGC CATTACCTGG CCTTTCAGTC AGAGAACTCG 
GCCTTCGATA GGGTCTCGGG CAAGGTGGCC AGCTCCGCAA GACAGCCCAT CCGCGGCTAC 
GTCCAGCCAG CAGAGCCTGT CCACACCATC ACGCTGGTGA CCTCAGACAC CCCAGAAAGC 
ATCTCCGAGG GCAGCTGGGT GGGTCCAGCC CCCCAGACTG TCACAAAGCC TCCCCCCTCA 
AAGGTTCTAG AAGGTGAAAG AAGAGACACC CCGGTTCTCC ATATTTGCGA ATCCAAAGCA 
GAAGATGTGC TCTTCTCTGA TGCTCTGGAG AAAACCAGGA AGACCCTTGC AGTTTTGGAG 
GATCGTGGGT CTGGGAGAAG CCAGGAAGCC CCCTCTGGAA CTGAGGACTT GAGTCAGCCC 
GCTGTTGGCA TAGTTACCGC GGAACCTCAG AAGGAATCAG AAAGCCTGGC CCACCCGCCT 
ATGGCTCAGC AGCAGCCCAC TGAGAGGATG GGCAGGAGTG AGATGGTCAT GTATGTTCAG 
AGCGAGGCTG TGTCCCAAGG TCACAGGAAG GAGGTGCCCA CGAGGAAACA CAGGGTCCTC 
ACCCGCTCCC TGTCCGACTA CACGGGCCCT CCACAGCTCC AGGCTCTGAA GGCCAAGGCC 
CCGGCTCCCA AGCGAGATGC CGAGTCACAG ACGTCCAAGG CCGAGTTGGA ACTGGGCCTG 
CTGGACACGA AGGTCTCGGT CGCCCAGCTC CGCAATGCGT TCCTGGAGTC GGCCAGGGCC 
AGCAGGAAAC CCGAGCTCCA TTCTCGGGTT GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG 
CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC TATTTTTCTC CTGGTGAAAA TAGAAAGACT 
TCCGAGAGAT TTAGAACTCA ACCTATAACC TCGGCAGAAC GAAAGGAATC GGATAGGTCA 
ACTTCAAATT CAGAAATGCC AGCTGCCGAG GATGAAGAAA AGGTCGATGA GCGAGCGAGG 
CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC AGGGAGATGG AGAAGTCGTT TGACGAGAAG 
AGCGTCCCAA AGCGCCGCTC CCGCAATGCG GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG 
GACCGGTCCC ACACGCAGCC CGTCACCACC GAGGAGGTGG TCATTGCAGC CACATTGCAG 
GCGTCTGCTC ACCAAAAGGC ACTAGCCAGA GACCAGACAA ACGAGAGCAA AGATTCTGCT 
GAGCAGGGAG AACCTGACTC CTCCACTCTG AGCTTAGCGG AGAAGCTGGC CTTGTTTAAC 
AAACTATCTC AGCCGGTCTC AAAAGCCATT TCAACACGGA ACAGATTAGA CATGAGACAG 
AGGAGGATGA ATGCTCGTTA TCAAACCCAG CCGGTCACGC TTGGAGAAGT GGAACAGGTG 
CAGAGTGGCA AGCTCATGGC TTTCTCTCCC ACCATTAACA CTTCCGTGTC CACCGTGGCA 
TCTACCGTCC CCCCCATGTA TGCAGGAAAT CTTCGGACAA AGCCACTTCC AGATGACAGC 
TTTGGTGCCA CTGAGCAGAA GTTTGCTTCT TCGCTAGAAA ACTCCGACTC CCCAGTTAGA 
AGCATCCTGA AATCCCAAGG CTGGCAGCCC TCGGTCGAGG GTGCTGGGAG CAAAGCAATG 
TTGAGAGAAT TTGAAGAGAC AGAACGCAAG GGAGGCTTGA CAGGTGGAGA TGGCGGGGTT 
ACAAAGTATG GGTCCTTCGA GGAAGCAGAG CTGTCCTACC CCGTCCTCAG CAGAGTCCGG 
GAGGGAGACA ACCATAAAGA GGCCATCTAT GCCCTTCCGA GGAAAGGAAG CCTGGAGCTC 
GCCCATCCTC CCATCGCCCA GCTTGGTGAT GACCTGAAGG AATTTTCCAC CCCAAAGAGC 
ACCATGCAGG CAAGCCCAGA CTGGAAGGAG AGGCAGCTCT TTGAAGAGAA GGTGGACTTG 
GAGAATGTCA CAAAGAGGAA GTTTTCGCTG AAAGCCGCGG AGTTCGGGGA ACCCACTTCT 
GAGCAGACCG GCGCGGCTGC TGGGAAACCG GCTGCTCCGA CTGCAACCCC GGTGTCCTGG 
AAGCCGCAGG ATCCCTCCGA ACAGCCTCAG GAGAAGCGGT ATCAGAGCCC GTGTGCGATG 
TTTGCTGCTG GAGAGATCAA AGCCCCGGCG GTGGAGGGCA GCCTCGACTC CCCCAGCAAA 
ACCATGTCCA TTAAAGAAAG ATTAGCACTG TTGAAGAAGA GCGGTGAGGA AGACTGGAGA 
AACAGACTCA ACAGAAAGCA GGAATACGGC AAAGCCTCCA TCACCAGCAG CCTGCACATC 
CAAGAGACGG AGCAGTCACT CAAGAAGAAG CGGGTCACAG AAAGTCGAGA GAGCCAGATG 
ACTATTGAAG AGAGAAAGCA TCTCATCACC GTGAGAGAGG ACGCCTGGAA GACCAGGGGC 
AAAGGAGCAG CCAACGACTC TACCCAGTTT ACTGTGGCCG GCAGGATGGT GAAGCGAGGT 
CTGGCGTCCC CCACTGCCAT CACCCCGGTA GCATCCCCTG TTTCCAGCAA AGCAAGGGGC 
ACTACACCAG TTTCCAGACC CCTGGAAGAT ATCGAAGCCA GACCAGACAT GCAGTTAGAG 
TCGGACCTCA AGCTGGACAG GCTGGAAACC TTCCTAAGAA GGCTGAATAA CAAAGTTGGT 
GGGATGCAAG AAACAGTCCT CACTGTCACT GGGAAATCGG TGAAAGAGGT GATGAAGCCG 
GACGATGATG AAACCTTTGC CAAGTTTTAC CGCAGCGTGG ACTCCTCTCT ACCGAGAAGC 
CCCGTAGAGC TGGACGAGGA TTTCGATGTC ATTTTTGATC CTTATGCCCC CAGGTTGACA 
TCCTCGGTGG CTGAGCACAA GCGTGCCGTG CGGCCCAAGC GCCGGGTCCA GGCTTCCAAA 
AACCCCCTGA AGATGCTGGC CGCGAGGGAA GATCTCCTGC AGGAGTACAC TGAGCAGAGA 
CTGAACGTGG CCTTCGTGGA GTCAAAGCGG ATGAAAGTCG AAAAGTTGTC CGCCAACTCC 
AGCTTCTCAG AAGTCACCCT GGCGGGGTTA GCCAGCAAAG AAAACTTCAG CAACGTCAGC 
CTGCGGAGCG TCAACCTGAC GGAACAGAAT TCCAACAACA GTGCAGTGCC CTACAAGAAG 
CTGATGCTGC TGCAGGTTAA AGGAAGAAGA CACGTGCAGA CGCGGCTGGT GGAGCCTCGC 
GCCCCCTCCC TCAACAGCGG GGACTGCTTC CTCCTGCTCT CGCCCCATCA CTGCTTCCTG 
TGGGTCGGAG AGTTCGCCAA CGTGATCGAG AAGGCGAAGG CCTCAGAACT TGCGAGTTTA 
ATTCAGACCA AGAGGGAACT TGGTTGTAGA GCAACTTACA TCCAGACTGT TGAAGAAGGA 
ATTAATACAC ACACACATGC AGCCAAAGAC TTCTGGAAGC TTCTGGGCGG CCAAGCCAGT 
TACCAATCCG CCGGAGACCC AAAGGAGGAT GAGCTCTATG AAACGGCCAT AATAGAGACC 
AACTGCATTT ACCGTCTGAT GGATGACAAA CTCGTTCCTG ATGATGACTA CTGGGGGAAG 
ATCCCAAAGT GCTCCCTCCT GCAATCAAAA GAGGTACTGG TGTTTGATTT TGGGAGTGAA 
GTTTACGTGT GGCATGGAAA AGAAGTCACA TTAGCACAAC GGAAAATCGC CTTTCAGCTG 
GCAAAGCACT TGTGGAATGG AACCTTTGAC TACGAGAATT GTGACATTAA CCCGCTGGAT 
CCTGGGGAAT GCAATCCCCT CATTCCCAGG AAAGGACAGG GGCGGCCCGA CTGGGCAATA 
TTTGGGAGAC TTACAGAACA CAATGAGACG ATCTTGTTCA AAGAGAAATT CCTTGATTGG 
ACGGAACTGA AGAGACCTAA CGAGAAGAAC GCCAGCGAAC TCGCGCAGCA CAAGGACGAT 
GCCCGGGCAG AGGTCAAGCC TTACGACGTG ACGCGGATGG TGCCCGTGCC CCAGACAACA 
GCCGGCACCG TGCTGGATGG GGTCAACGTG GGCCGAGGCT ACGGGCTGGT GGAAGGGGAC 
GACCGGAGGC AGTTCGAGAT CGCCAGCATC TCAGTGGACG TCTGGCACAT CCTGGAGTTC 
GACTACAGCA GGCTCCCCAA GCAGAGCATC GGGCAGTTCC ACGAAGGCGA TGCCTACGTG 
GTGAAGTGGA AGTTCATCGT GAGCACTGCA GTGGGAAGCC GGCAGAAGGG GGAGCACTCG 
GTCAGGGTGG CTGGCAAAGA GAAATGTGTC TACTTCTTCT GGCAAGGCCG CCAGTCGACC 
GTGAGTGAGA AGGGCACATC AGCTCTGATG ACAGTGGAGC TGGACGAGGA AAGGGGGGCC 
CAGGTCCAGG TCCTGCAGGG CAAGGAGCCC CCCTGTTTCC TGCAGTGCTT CCAGGGGGGG 
ATGGTGGTCC ACTCCGGGAG ACGGGAGGAG GAGGAAGAGA ACACACAGAG CGAGTGGCGG 
CTGTACTGTG TGCGCGGAGA GGTGCCTGTG GAGGGGAACT TGCTGGAGGT GGCCTGTCAC 
TGCAGCAGCC TCAGGTCTAG GACGTCCATG GTTGTCCTCA ACGTCCACAA AGCCCTCATC 
TACCTGTGGC ATGGATGCAA AGCCCAGGCC CACACGAAGG AGGTCGGACG GACTGCAGCC 
AATAAAATCA AGGACCAATG TCCCCTGGAG GCCGGGCTGC ACAGTAGCAG CAAAGTGACA 
ATTCATGAGT GTGATGAAGG CTCGGAGCCC CTGGGGTTCT GGGACGCTCT GGGGAGGAGA 
GACCGGAAAG CCTACGACTG CATGCTTCAA GATCCTGGAA ATTTTAACTT CACACCCCGC 
CTGTTCATCC TCAGCAGCTC CTCTGGTGAC TTCTCAGCCA CAGAGTTCAT GTATCCCGCC 
CGAGACCCCT CTGTGGTCAA CTCTATGCCC TTCCTGCAGG AAGACCTATA CAGCGCCCCA 
CAGCCAGCAC TCTTCCTTGT TGACAATCAC CACGAGGTGT ACCTCTGGCA AGGCTGGTGG 
CCCATCGAGA ACAAGATCAC GGGCTCCGCG CGCATCCGCT GGGCCTCGGA CCGGAAGAGC 
GCCATGGAGA CAGTGCTGCA GTACTGCCGA GGGAAAAACC TGAAGAAGCC GCCCCCCAAG 
TCTTACCTCA TCCACGCTGG CCTGGAACCC CTGACCTTCA CCAACATGTT CCCCAGCTGG 
GAGCACAGAG AAGACATTGC AGAGATCACG GAGATGGACA CGGAAGTTTC AAATCAGATC 
ACCCTAGTGG AGGATGTCTT AGCCAAACTG TGTAAGACCA TTTACCCACT GGCCGACCTC 
CTAGCCAGGC CGCTCCCTGA GGGAGTTGAC CCTCTGAAGC TGGAGATCTA TCTCACCGAC 
GAGGACTTCG AGTTTGCACT AGACATGACC CGAGACGAGT ACAATGCACT GCCCGCTTGG 
AAGCAGGTGA ACCTGAAGAA AGCGAAAGGT CTGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000280.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856048.1 Bos taurus supervillin (SVIL), transcript variant X18, mRNA.

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CloneID	OBa221414
Clone ID Related Accession (Same CDS sequence)	XM_025000280.1
Accession Version	XM_025000280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTGTCAAC TTCAAATTCA GAAATGCCAG CTGCCGAGGA TGAAGAAAAG 
GTCGATGAGC GAGCGAGGCT GAGCGTCGCT GCTAAGAGGC TGCTCTTCAG GGAGATGGAG 
AAGTCGTTTG ACGAGAAGAG CGTCCCAAAG CGCCGCTCCC GCAATGCGGC CGTGGAGCAG 
AGGCTGCGGC GCCTCCAGGA CCGGTCCCAC ACGCAGCCCG TCACCACCGA GGAGGTGGTC 
ATTGCAGCCA CATTGCAGGC GTCTGCTCAC CAAAAGGCAC TAGCCAGAGA CCAGACAAAC 
GAGAGCAAAG ATTCTGCTGA GCAGGGAGAA CCTGACTCCT CCACTCTGAG CTTAGCGGAG 
AAGCTGGCCT TGTTTAACAA ACTATCTCAG CCGGTCTCAA AAGCCATTTC AACACGGAAC 
AGATTAGACA TGAGACAGAG GAGGATGAAT GCTCGTTATC AAACCCAGCC GGTCACGCTT 
GGAGAAGTGG AACAGGTGCA GAGTGGCAAG CTCATGGCTT TCTCTCCCAC CATTAACACT 
TCCGTGTCCA CCGTGGCATC TACCGTCCCC CCCATGTATG CAGGAAATCT TCGGACAAAG 
CCACTTCCAG ATGACAGCTT TGGTGCCACT GAGCAGAAGT TTGCTTCTTC GCTAGAAAAC 
TCCGACTCCC CAGTTAGAAG CATCCTGAAA TCCCAAGGCT GGCAGCCCTC GGTCGAGGGT 
GCTGGGAGCA AAGCAATGTT GAGAGAATTT GAAGAGACAG AACGCAAGGG AGGCTTGACA 
GGTGGAGATG GCGGGGTTAC AAAGTATGGG TCCTTCGAGG AAGCAGAGCT GTCCTACCCC 
GTCCTCAGCA GAGTCCGGGA GGGAGACAAC CATAAAGAGG CCATCTATGC CCTTCCGAGG 
AAAGGAAGCC TGGAGCTCGC CCATCCTCCC ATCGCCCAGC TTGGTGATGA CCTGAAGGAA 
TTTTCCACCC CAAAGAGCAC CATGCAGGCA AGCCCAGACT GGAAGGAGAG GCAGCTCTTT 
GAAGAGAAGG TGGACTTGGA GAATGTCACA AAGAGGAAGT TTTCGCTGAA AGCCGCGGAG 
TTCGGGGAAC CCACTTCTGA GCAGACCGGC GCGGCTGCTG GGAAACCGGC TGCTCCGACT 
GCAACCCCGG TGTCCTGGAA GCCGCAGGAT CCCTCCGAAC AGCCTCAGGA GAAGCGGTAT 
CAGAGCCCGT GTGCGATGTT TGCTGCTGGA GAGATCAAAG CCCCGGCGGT GGAGGGCAGC 
CTCGACTCCC CCAGCAAAAC CATGTCCATT AAAGAAAGAT TAGCACTGTT GAAGAAGAGC 
GGTGAGGAAG ACTGGAGAAA CAGACTCAAC AGAAAGCAGG AATACGGCAA AGCCTCCATC 
ACCAGCAGCC TGCACATCCA AGAGACGGAG CAGTCACTCA AGAAGAAGCG GGTCACAGAA 
AGTCGAGAGA GCCAGATGAC TATTGAAGAG AGAAAGCATC TCATCACCGT GAGAGAGGAC 
GCCTGGAAGA CCAGGGGCAA AGGAGCAGCC AACGACTCTA CCCAGTTTAC TGTGGCCGGC 
AGGATGGTGA AGCGAGGTCT GGCGTCCCCC ACTGCCATCA CCCCGGTAGC ATCCCCTGTT 
TCCAGCAAAG CAAGGGGCAC TACACCAGTT TCCAGACCCC TGGAAGATAT CGAAGCCAGA 
CCAGACATGC AGTTAGAGTC GGACCTCAAG CTGGACAGGC TGGAAACCTT CCTAAGAAGG 
CTGAATAACA AAGTTGGTGG GATGCAAGAA ACAGTCCTCA CTGTCACTGG GAAATCGGTG 
AAAGAGGTGA TGAAGCCGGA CGATGATGAA ACCTTTGCCA AGTTTTACCG CAGCGTGGAC 
TCCTCTCTAC CGAGAAGCCC CGTAGAGCTG GACGAGGATT TCGATGTCAT TTTTGATCCT 
TATGCCCCCA GGTTGACATC CTCGGTGGCT GAGCACAAGC GTGCCGTGCG GCCCAAGCGC 
CGGGTCCAGG CTTCCAAAAA CCCCCTGAAG ATGCTGGCCG CGAGGGAAGA TCTCCTGCAG 
GAGTACACTG AGCAGAGACT GAACGTGGCC TTCGTGGAGT CAAAGCGGAT GAAAGTCGAA 
AAGTTGTCCG CCAACTCCAG CTTCTCAGAA GTCACCCTGG CGGGGTTAGC CAGCAAAGAA 
AACTTCAGCA ACGTCAGCCT GCGGAGCGTC AACCTGACGG AACAGAATTC CAACAACAGT 
GCAGTGCCCT ACAAGAAGCT GATGCTGCTG CAGGTTAAAG GAAGAAGACA CGTGCAGACG 
CGGCTGGTGG AGCCTCGCGC CCCCTCCCTC AACAGCGGGG ACTGCTTCCT CCTGCTCTCG 
CCCCATCACT GCTTCCTGTG GGTCGGAGAG TTCGCCAACG TGATCGAGAA GGCGAAGGCC 
TCAGAACTTG CGAGTTTAAT TCAGACCAAG AGGGAACTTG GTTGTAGAGC AACTTACATC 
CAGACTGTTG AAGAAGGAAT TAATACACAC ACACATGCAG CCAAAGACTT CTGGAAGCTT 
CTGGGCGGCC AAGCCAGTTA CCAATCCGCC GGAGACCCAA AGGAGGATGA GCTCTATGAA 
ACGGCCATAA TAGAGACCAA CTGCATTTAC CGTCTGATGG ATGACAAACT CGTTCCTGAT 
GATGACTACT GGGGGAAGAT CCCAAAGTGC TCCCTCCTGC AATCAAAAGA GGTACTGGTG 
TTTGATTTTG GGAGTGAAGT TTACGTGTGG CATGGAAAAG AAGTCACATT AGCACAACGG 
AAAATCGCCT TTCAGCTGGC AAAGCACTTG TGGAATGGAA CCTTTGACTA CGAGAATTGT 
GACATTAACC CGCTGGATCC TGGGGAATGC AATCCCCTCA TTCCCAGGAA AGGACAGGGG 
CGGCCCGACT GGGCAATATT TGGGAGACTT ACAGAACACA ATGAGACGAT CTTGTTCAAA 
GAGAAATTCC TTGATTGGAC GGAACTGAAG AGACCTAACG AGAAGAACGC CAGCGAACTC 
GCGCAGCACA AGGACGATGC CCGGGCAGAG GTCAAGCCTT ACGACGTGAC GCGGATGGTG 
CCCGTGCCCC AGACAACAGC CGGCACCGTG CTGGATGGGG TCAACGTGGG CCGAGGCTAC 
GGGCTGGTGG AAGGGGACGA CCGGAGGCAG TTCGAGATCG CCAGCATCTC AGTGGACGTC 
TGGCACATCC TGGAGTTCGA CTACAGCAGG CTCCCCAAGC AGAGCATCGG GCAGTTCCAC 
GAAGGCGATG CCTACGTGGT GAAGTGGAAG TTCATCGTGA GCACTGCAGT GGGAAGCCGG 
CAGAAGGGGG AGCACTCGGT CAGGGTGGCT GGCAAAGAGA AATGTGTCTA CTTCTTCTGG 
CAAGGCCGCC AGTCGACCGT GAGTGAGAAG GGCACATCAG CTCTGATGAC AGTGGAGCTG 
GACGAGGAAA GGGGGGCCCA GGTCCAGGTC CTGCAGGGCA AGGAGCCCCC CTGTTTCCTG 
CAGTGCTTCC AGGGGGGGAT GGTGGTCCAC TCCGGGAGAC GGGAGGAGGA GGAAGAGAAC 
ACACAGAGCG AGTGGCGGCT GTACTGTGTG CGCGGAGAGG TGCCTGTGGA GGGGAACTTG 
CTGGAGGTGG CCTGTCACTG CAGCAGCCTC AGGTCTAGGA CGTCCATGGT TGTCCTCAAC 
GTCCACAAAG CCCTCATCTA CCTGTGGCAT GGATGCAAAG CCCAGGCCCA CACGAAGGAG 
GTCGGACGGA CTGCAGCCAA TAAAATCAAG GACCAATGTC CCCTGGAGGC CGGGCTGCAC 
AGTAGCAGCA AAGTGACAAT TCATGAGTGT GATGAAGGCT CGGAGCCCCT GGGGTTCTGG 
GACGCTCTGG GGAGGAGAGA CCGGAAAGCC TACGACTGCA TGCTTCAAGA TCCTGGAAAT 
TTTAACTTCA CACCCCGCCT GTTCATCCTC AGCAGCTCCT CTGGTGACTT CTCAGCCACA 
GAGTTCATGT ATCCCGCCCG AGACCCCTCT GTGGTCAACT CTATGCCCTT CCTGCAGGAA 
GACCTATACA GCGCCCCACA GCCAGCACTC TTCCTTGTTG ACAATCACCA CGAGGTGTAC 
CTCTGGCAAG GCTGGTGGCC CATCGAGAAC AAGATCACGG GCTCCGCGCG CATCCGCTGG 
GCCTCGGACC GGAAGAGCGC CATGGAGACA GTGCTGCAGT ACTGCCGAGG GAAAAACCTG 
AAGAAGCCGC CCCCCAAGTC TTACCTCATC CACGCTGGCC TGGAACCCCT GACCTTCACC 
AACATGTTCC CCAGCTGGGA GCACAGAGAA GACATTGCAG AGATCACGGA GATGGACACG 
GAAGTTTCAA ATCAGATCAC CCTAGTGGAG GATGTCTTAG CCAAACTGTG TAAGACCATT 
TACCCACTGG CCGACCTCCT AGCCAGGCCG CTCCCTGAGG GAGTTGACCC TCTGAAGCTG 
GAGATCTATC TCACCGACGA GGACTTCGAG TTTGCACTAG ACATGACCCG AGACGAGTAC 
AATGCACTGC CCGCTTGGAA GCAGGTGAAC CTGAAGAAAG CGAAAGGTCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856048.1
CDS	356..6952
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000280.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000280.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGAGTTAA AGTTAGAGAA 
AAATTGGTGC GAGAGGAGAG CGCTCGCAGC AGCCCTGAAC TTACCTCCGA GTCCTTAACT 
CAGAGGAGAC ACCAGACGGC ACCAGGCCAT TACCTGGCCT TTCAGTCAGA GAACTCGGCC 
TTCGATAGGG TCTCGGGCAA GGTGGCCAGC TCCGCAAGAC AGCCCATCCG CGGCTACGTC 
CAGCCAGCAG AGCCTGTCCA CACCATCACG CTGGTGACCT CAGACACCCC AGAAAGCATC 
TCCGAGGGCA GCTGGGTGGG TCCAGCCCCC CAGACTGTCA CAAAGCCTCC CCCCTCAAAG 
GTTCTAGAAG GTGAAAGAAG AGACACCCCG GTTCTCCATA TTTGCGAATC CAAAGCAGAA 
GATGTGCTCT TCTCTGATGC TCTGGAGAAA ACCAGGAAGA CCCTTGCAGT TTTGGAGGAT 
CGTGGGTCTG GGAGAAGCCA GGAAGCCCCC TCTGGAACTG AGGACTTGAG TCAGCCCGCT 
GTTGGCATAG TTACCGCGGA ACCTCAGAAG GAATCAGAAA GCCTGGCCCA CCCGCCTATG 
GCTCAGCAGC AGCCCACTGA GAGGATGGGC AGGAGTGAGA TGGTCATGTA TGTTCAGAGC 
GAGGCTGTGT CCCAAGGTCA CAGGAAGGAG GTGCCCACGA GGAAACACAG GGTCCTCACC 
CGCTCCCTGT CCGACTACAC GGGCCCTCCA CAGCTCCAGG CTCTGAAGGC CAAGGCCCCG 
GCTCCCAAGC GAGATGCCGA GTCACAGACG TCCAAGGCCG AGTTGGAACT GGGCCTGCTG 
GACACGAAGG TCTCGGTCGC CCAGCTCCGC AATGCGTTCC TGGAGTCGGC CAGGGCCAGC 
AGGAAACCCG AGCTGTCAAC TTCAAATTCA GAAATGCCAG CTGCCGAGGA TGAAGAAAAG 
GTCGATGAGC GAGCGAGGCT GAGCGTCGCT GCTAAGAGGC TGCTCTTCAG GGAGATGGAG 
AAGTCGTTTG ACGAGAAGAG CGTCCCAAAG CGCCGCTCCC GCAATGCGGC CGTGGAGCAG 
AGGCTGCGGC GCCTCCAGGA CCGGTCCCAC ACGCAGCCCG TCACCACCGA GGAGGTGGTC 
ATTGCAGCCA CATTGCAGGC GTCTGCTCAC CAAAAGGCAC TAGCCAGAGA CCAGACAAAC 
GAGAGCAAAG ATTCTGCTGA GCAGGGAGAA CCTGACTCCT CCACTCTGAG CTTAGCGGAG 
AAGCTGGCCT TGTTTAACAA ACTATCTCAG CCGGTCTCAA AAGCCATTTC AACACGGAAC 
AGATTAGACA TGAGACAGAG GAGGATGAAT GCTCGTTATC AAACCCAGCC GGTCACGCTT 
GGAGAAGTGG AACAGGTGCA GAGTGGCAAG CTCATGGCTT TCTCTCCCAC CATTAACACT 
TCCGTGTCCA CCGTGGCATC TACCGTCCCC CCCATGTATG CAGGAAATCT TCGGACAAAG 
CCACTTCCAG ATGACAGCTT TGGTGCCACT GAGCAGAAGT TTGCTTCTTC GCTAGAAAAC 
TCCGACTCCC CAGTTAGAAG CATCCTGAAA TCCCAAGGCT GGCAGCCCTC GGTCGAGGGT 
GCTGGGAGCA AAGCAATGTT GAGAGAATTT GAAGAGACAG AACGCAAGGG AGGCTTGACA 
GGTGGAGATG GCGGGGTTAC AAAGTATGGG TCCTTCGAGG AAGCAGAGCT GTCCTACCCC 
GTCCTCAGCA GAGTCCGGGA GGGAGACAAC CATAAAGAGG CCATCTATGC CCTTCCGAGG 
AAAGGAAGCC TGGAGCTCGC CCATCCTCCC ATCGCCCAGC TTGGTGATGA CCTGAAGGAA 
TTTTCCACCC CAAAGAGCAC CATGCAGGCA AGCCCAGACT GGAAGGAGAG GCAGCTCTTT 
GAAGAGAAGG TGGACTTGGA GAATGTCACA AAGAGGAAGT TTTCGCTGAA AGCCGCGGAG 
TTCGGGGAAC CCACTTCTGA GCAGACCGGC GCGGCTGCTG GGAAACCGGC TGCTCCGACT 
GCAACCCCGG TGTCCTGGAA GCCGCAGGAT CCCTCCGAAC AGCCTCAGGA GAAGCGGTAT 
CAGAGCCCGT GTGCGATGTT TGCTGCTGGA GAGATCAAAG CCCCGGCGGT GGAGGGCAGC 
CTCGACTCCC CCAGCAAAAC CATGTCCATT AAAGAAAGAT TAGCACTGTT GAAGAAGAGC 
GGTGAGGAAG ACTGGAGAAA CAGACTCAAC AGAAAGCAGG AATACGGCAA AGCCTCCATC 
ACCAGCAGCC TGCACATCCA AGAGACGGAG CAGTCACTCA AGAAGAAGCG GGTCACAGAA 
AGTCGAGAGA GCCAGATGAC TATTGAAGAG AGAAAGCATC TCATCACCGT GAGAGAGGAC 
GCCTGGAAGA CCAGGGGCAA AGGAGCAGCC AACGACTCTA CCCAGTTTAC TGTGGCCGGC 
AGGATGGTGA AGCGAGGTCT GGCGTCCCCC ACTGCCATCA CCCCGGTAGC ATCCCCTGTT 
TCCAGCAAAG CAAGGGGCAC TACACCAGTT TCCAGACCCC TGGAAGATAT CGAAGCCAGA 
CCAGACATGC AGTTAGAGTC GGACCTCAAG CTGGACAGGC TGGAAACCTT CCTAAGAAGG 
CTGAATAACA AAGTTGGTGG GATGCAAGAA ACAGTCCTCA CTGTCACTGG GAAATCGGTG 
AAAGAGGTGA TGAAGCCGGA CGATGATGAA ACCTTTGCCA AGTTTTACCG CAGCGTGGAC 
TCCTCTCTAC CGAGAAGCCC CGTAGAGCTG GACGAGGATT TCGATGTCAT TTTTGATCCT 
TATGCCCCCA GGTTGACATC CTCGGTGGCT GAGCACAAGC GTGCCGTGCG GCCCAAGCGC 
CGGGTCCAGG CTTCCAAAAA CCCCCTGAAG ATGCTGGCCG CGAGGGAAGA TCTCCTGCAG 
GAGTACACTG AGCAGAGACT GAACGTGGCC TTCGTGGAGT CAAAGCGGAT GAAAGTCGAA 
AAGTTGTCCG CCAACTCCAG CTTCTCAGAA GTCACCCTGG CGGGGTTAGC CAGCAAAGAA 
AACTTCAGCA ACGTCAGCCT GCGGAGCGTC AACCTGACGG AACAGAATTC CAACAACAGT 
GCAGTGCCCT ACAAGAAGCT GATGCTGCTG CAGGTTAAAG GAAGAAGACA CGTGCAGACG 
CGGCTGGTGG AGCCTCGCGC CCCCTCCCTC AACAGCGGGG ACTGCTTCCT CCTGCTCTCG 
CCCCATCACT GCTTCCTGTG GGTCGGAGAG TTCGCCAACG TGATCGAGAA GGCGAAGGCC 
TCAGAACTTG CGAGTTTAAT TCAGACCAAG AGGGAACTTG GTTGTAGAGC AACTTACATC 
CAGACTGTTG AAGAAGGAAT TAATACACAC ACACATGCAG CCAAAGACTT CTGGAAGCTT 
CTGGGCGGCC AAGCCAGTTA CCAATCCGCC GGAGACCCAA AGGAGGATGA GCTCTATGAA 
ACGGCCATAA TAGAGACCAA CTGCATTTAC CGTCTGATGG ATGACAAACT CGTTCCTGAT 
GATGACTACT GGGGGAAGAT CCCAAAGTGC TCCCTCCTGC AATCAAAAGA GGTACTGGTG 
TTTGATTTTG GGAGTGAAGT TTACGTGTGG CATGGAAAAG AAGTCACATT AGCACAACGG 
AAAATCGCCT TTCAGCTGGC AAAGCACTTG TGGAATGGAA CCTTTGACTA CGAGAATTGT 
GACATTAACC CGCTGGATCC TGGGGAATGC AATCCCCTCA TTCCCAGGAA AGGACAGGGG 
CGGCCCGACT GGGCAATATT TGGGAGACTT ACAGAACACA ATGAGACGAT CTTGTTCAAA 
GAGAAATTCC TTGATTGGAC GGAACTGAAG AGACCTAACG AGAAGAACGC CAGCGAACTC 
GCGCAGCACA AGGACGATGC CCGGGCAGAG GTCAAGCCTT ACGACGTGAC GCGGATGGTG 
CCCGTGCCCC AGACAACAGC CGGCACCGTG CTGGATGGGG TCAACGTGGG CCGAGGCTAC 
GGGCTGGTGG AAGGGGACGA CCGGAGGCAG TTCGAGATCG CCAGCATCTC AGTGGACGTC 
TGGCACATCC TGGAGTTCGA CTACAGCAGG CTCCCCAAGC AGAGCATCGG GCAGTTCCAC 
GAAGGCGATG CCTACGTGGT GAAGTGGAAG TTCATCGTGA GCACTGCAGT GGGAAGCCGG 
CAGAAGGGGG AGCACTCGGT CAGGGTGGCT GGCAAAGAGA AATGTGTCTA CTTCTTCTGG 
CAAGGCCGCC AGTCGACCGT GAGTGAGAAG GGCACATCAG CTCTGATGAC AGTGGAGCTG 
GACGAGGAAA GGGGGGCCCA GGTCCAGGTC CTGCAGGGCA AGGAGCCCCC CTGTTTCCTG 
CAGTGCTTCC AGGGGGGGAT GGTGGTCCAC TCCGGGAGAC GGGAGGAGGA GGAAGAGAAC 
ACACAGAGCG AGTGGCGGCT GTACTGTGTG CGCGGAGAGG TGCCTGTGGA GGGGAACTTG 
CTGGAGGTGG CCTGTCACTG CAGCAGCCTC AGGTCTAGGA CGTCCATGGT TGTCCTCAAC 
GTCCACAAAG CCCTCATCTA CCTGTGGCAT GGATGCAAAG CCCAGGCCCA CACGAAGGAG 
GTCGGACGGA CTGCAGCCAA TAAAATCAAG GACCAATGTC CCCTGGAGGC CGGGCTGCAC 
AGTAGCAGCA AAGTGACAAT TCATGAGTGT GATGAAGGCT CGGAGCCCCT GGGGTTCTGG 
GACGCTCTGG GGAGGAGAGA CCGGAAAGCC TACGACTGCA TGCTTCAAGA TCCTGGAAAT 
TTTAACTTCA CACCCCGCCT GTTCATCCTC AGCAGCTCCT CTGGTGACTT CTCAGCCACA 
GAGTTCATGT ATCCCGCCCG AGACCCCTCT GTGGTCAACT CTATGCCCTT CCTGCAGGAA 
GACCTATACA GCGCCCCACA GCCAGCACTC TTCCTTGTTG ACAATCACCA CGAGGTGTAC 
CTCTGGCAAG GCTGGTGGCC CATCGAGAAC AAGATCACGG GCTCCGCGCG CATCCGCTGG 
GCCTCGGACC GGAAGAGCGC CATGGAGACA GTGCTGCAGT ACTGCCGAGG GAAAAACCTG 
AAGAAGCCGC CCCCCAAGTC TTACCTCATC CACGCTGGCC TGGAACCCCT GACCTTCACC 
AACATGTTCC CCAGCTGGGA GCACAGAGAA GACATTGCAG AGATCACGGA GATGGACACG 
GAAGTTTCAA ATCAGATCAC CCTAGTGGAG GATGTCTTAG CCAAACTGTG TAAGACCATT 
TACCCACTGG CCGACCTCCT AGCCAGGCCG CTCCCTGAGG GAGTTGACCC TCTGAAGCTG 
GAGATCTATC TCACCGACGA GGACTTCGAG TTTGCACTAG ACATGACCCG AGACGAGTAC 
AATGCACTGC CCGCTTGGAA GCAGGTGAAC CTGAAGAAAG CGAAAGGTCT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000282.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856050.1 Bos taurus supervillin (SVIL), transcript variant X20, mRNA.

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CloneID	OBa221415
Clone ID Related Accession (Same CDS sequence)	XM_025000282.1
Accession Version	XM_025000282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTATC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCACTT 
ATCAACTGGC CATCCAGAGT TAAAGTTAGA GAAAAATTGG TGCGAGAGGA GAGCGCTCGC 
AGCAGCCCTG AACTTACCTC CGAGTCCTTA ACTCAGAGGA GACACCAGAC GGCACCAGGC 
CATTACCTGG CCTTTCAGTC AGAGAACTCG GCCTTCGATA GGGTCTCGGG CAAGGTGGCC 
AGCTCCGCAA GACAGCCCAT CCGCGGCTAC GTCCAGCCAG CAGAGCCTGT CCACACCATC 
ACGCTGGTGA CCTCAGACAC CCCAGAAAGC ATCTCCGAGG GCAGCTGGGT GGGTCCAGCC 
CCCCAGACTG TCACAAAGCC TCCCCCCTCA AAGGTTCTAG AAGGTGAAAG AAGAGACACC 
CCGGTTCTCC ATATTTGCGA ATCCAAAGCA GAAGATGTGC TCTTCTCTGA TGCTCTGGAG 
AAAACCAGGA AGACCCTTGC AGTTTTGGAG GATCGTGGGT CTGGGAGAAG CCAGGAAGCC 
CCCTCTGGAA CTGAGGACTT GAGTCAGCCC GCTGTTGGCA TAGTTACCGC GGAACCTCAG 
AAGGAATCAG AAAGCCTGGC CCACCCGCCT ATGGCTCAGC AGCAGCCCAC TGAGAGGATG 
GGCAGGAGTG AGATGGTCAT GTATGTTCAG AGCGAGGCTG TGTCCCAAGG TCACAGGAAG 
GAGGTGCCCA CGAGGAAACA CAGGGTCCTC ACCCGCTCCC TGTCCGACTA CACGGGCCCT 
CCACAGCTCC AGGCTCTGAA GGCCAAGGCC CCGGCTCCCA AGCGAGATGC CGAGTCACAG 
ACGTCCAAGG CCGAGTTGGA ACTGGGCCTG CTGGACACGA AGGTCTCGGT CGCCCAGCTC 
CGCAATGCGT TCCTGGAGTC GGCCAGGGCC AGCAGGAAAC CCGAGCTGTC AACTTCAAAT 
TCAGAAATGC CAGCTGCCGA GGATGAAGAA AAGGTCGATG AGCGAGCGAG GCTGAGCGTC 
GCTGCTAAGA GGCTGCTCTT CAGGGAGATG GAGAAGTCGT TTGACGAGAA GAGCGTCCCA 
AAGCGCCGCT CCCGCAATGC GGCCGTGGAG CAGAGGCTGC GGCGCCTCCA GGACCGGTCC 
CACACGCAGC CCGTCACCAC CGAGGAGGTG GTCATTGCAG CCACTGAACC TACCCCCGCT 
TCGTGCTCAG TGGCCACCCA CCCTGTAATG ACAAGACATC CTAGCCCCAC TGTAGCTAAG 
AGTCCCGTGC AGCCTGCCAG ATTGCAGGCG TCTGCTCACC AAAAGGCACT AGCCAGAGAC 
CAGACAAACG AGAGCAAAGA TTCTGCTGAG CAGGGAGAAC CTGACTCCTC CACTCTGAGC 
TTAGCGGAGA AGCTGGCCTT GTTTAACAAA CTATCTCAGC CGGTCTCAAA AGCCATTTCA 
ACACGGAACA GATTAGACAT GAGACAGAGG AGGATGAATG CTCGTTATCA AACCCAGCCG 
GTCACGCTTG GAGAAGTGGA ACAGGTGCAG AGTGGCAAGC TCATGGCTTT CTCTCCCACC 
ATTAACACTT CCGTGTCCAC CGTGGCATCT ACCGTCCCCC CCATGTATGC AGGAAATCTT 
CGGACAAAGC CACTTCCAGA TGACAGCTTT GGTGCCACTG AGCAGAAGTT TGCTTCTTCG 
CTAGAAAACT CCGACTCCCC AGTTAGAAGC ATCCTGAAAT CCCAAGGCTG GCAGCCCTCG 
GTCGAGGGTG CTGGGAGCAA AGCAATGTTG AGAGAATTTG AAGAGACAGA ACGCAAGGGA 
GGCTTGACAG GTGGAGATGG CGGGGTTACA AAGTATGGGT CCTTCGAGGA AGCAGAGCTG 
TCCTACCCCG TCCTCAGCAG AGTCCGGGAG GGAGACAACC ATAAAGAGGC CATCTATGCC 
CTTCCGAGGA AAGGAAGCCT GGAGCTCGCC CATCCTCCCA TCGCCCAGCT TGGTGATGAC 
CTGAAGGAAT TTTCCACCCC AAAGAGCACC ATGCAGGCAA GCCCAGACTG GAAGGAGAGG 
CAGCTCTTTG AAGAGAAGGT GGACTTGGAG AATGTCACAA AGAGGAAGTT TTCGCTGAAA 
GCCGCGGAGT TCGGGGAACC CACTTCTGAG CAGACCGGCG CGGCTGCTGG GAAACCGGCT 
GCTCCGACTG CAACCCCGGT GTCCTGGAAG CCGCAGGATC CCTCCGAACA GCCTCAGGAG 
AAGCGGTATC AGAGCCCGTG TGCGATGTTT GCTGCTGGAG AGATCAAAGC CCCGGCGGTG 
GAGGGCAGCC TCGACTCCCC CAGCAAAACC ATGTCCATTA AAGAAAGATT AGCACTGTTG 
AAGAAGAGCG GTGAGGAAGA CTGGAGAAAC AGACTCAACA GAAAGCAGGA ATACGGCAAA 
GCCTCCATCA CCAGCAGCCT GCACATCCAA GAGACGGAGC AGTCACTCAA GAAGAAGGAA 
GAAGGAGGAT TTACAGATGA TAATGACATT TCTAATCTGC TTTGGGAACC TGTATACGCT 
TCTACTTATT CTCCTGCTCT ACCTGCTGCC CATAAATACC TGTCTTTTGT ATCTATTAAT 
CAGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATTG AAGAGAGAAA GCATCTCATC 
ACCGTGAGAG AGGACGCCTG GAAGACCAGG GGCAAAGGAG CAGCCAACGA CTCTACCCAG 
TTTACTGTGG CCGGCAGGAT GGTGAAGCGA GGTCTGGCGT CCCCCACTGC CATCACCCCG 
GTAGCATCCC CTGTTTCCAG CAAAGCAAGG GGCACTACAC CAGTTTCCAG ACCCCTGGAA 
GATATCGAAG CCAGACCAGA CATGCAGTTA GAGTCGGACC TCAAGCTGGA CAGGCTGGAA 
ACCTTCCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AAGAAACAGT CCTCACTGTC 
ACTGGGAAAT CGGTGAAAGA GGTGATGAAG CCGGACGATG ATGAAACCTT TGCCAAGTTT 
TACCGCAGCG TGGACTCCTC TCTACCGAGA AGCCCCGTAG AGCTGGACGA GGATTTCGAT 
GTCATTTTTG ATCCTTATGC CCCCAGGTTG ACATCCTCGG TGGCTGAGCA CAAGCGTGCC 
GTGCGGCCCA AGCGCCGGGT CCAGGCTTCC AAAAACCCCC TGAAGATGCT GGCCGCGAGG 
GAAGATCTCC TGCAGGAGTA CACTGAGCAG AGACTGAACG TGGCCTTCGT GGAGTCAAAG 
CGGATGAAAG TCGAAAAGTT GTCCGCCAAC TCCAGCTTCT CAGAAGTCAC CCTGGCGGGG 
TTAGCCAGCA AAGAAAACTT CAGCAACGTC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ACAGTGCAGT GCCCTACAAG AAGCTGATGC TGCTGCAGGT TAAAGGAAGA 
AGACACGTGC AGACGCGGCT GGTGGAGCCT CGCGCCCCCT CCCTCAACAG CGGGGACTGC 
TTCCTCCTGC TCTCGCCCCA TCACTGCTTC CTGTGGGTCG GAGAGTTCGC CAACGTGATC 
GAGAAGGCGA AGGCCTCAGA ACTTGCGAGT TTAATTCAGA CCAAGAGGGA ACTTGGTTGT 
AGAGCAACTT ACATCCAGAC TGTTGAAGAA GGAATTAATA CACACACACA TGCAGCCAAA 
GACTTCTGGA AGCTTCTGGG CGGCCAAGCC AGTTACCAAT CCGCCGGAGA CCCAAAGGAG 
GATGAGCTCT ATGAAACGGC CATAATAGAG ACCAACTGCA TTTACCGTCT GATGGATGAC 
AAACTCGTTC CTGATGATGA CTACTGGGGG AAGATCCCAA AGTGCTCCCT CCTGCAATCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGGAGT GAAGTTTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCAC AACGGAAAAT CGCCTTTCAG CTGGCAAAGC ACTTGTGGAA TGGAACCTTT 
GACTACGAGA ATTGTGACAT TAACCCGCTG GATCCTGGGG AATGCAATCC CCTCATTCCC 
AGGAAAGGAC AGGGGCGGCC CGACTGGGCA ATATTTGGGA GACTTACAGA ACACAATGAG 
ACGATCTTGT TCAAAGAGAA ATTCCTTGAT TGGACGGAAC TGAAGAGACC TAACGAGAAG 
AACGCCAGCG AACTCGCGCA GCACAAGGAC GATGCCCGGG CAGAGGTCAA GCCTTACGAC 
GTGACGCGGA TGGTGCCCGT GCCCCAGACA ACAGCCGGCA CCGTGCTGGA TGGGGTCAAC 
GTGGGCCGAG GCTACGGGCT GGTGGAAGGG GACGACCGGA GGCAGTTCGA GATCGCCAGC 
ATCTCAGTGG ACGTCTGGCA CATCCTGGAG TTCGACTACA GCAGGCTCCC CAAGCAGAGC 
ATCGGGCAGT TCCACGAAGG CGATGCCTAC GTGGTGAAGT GGAAGTTCAT CGTGAGCACT 
GCAGTGGGAA GCCGGCAGAA GGGGGAGCAC TCGGTCAGGG TGGCTGGCAA AGAGAAATGT 
GTCTACTTCT TCTGGCAAGG CCGCCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGACGA GGAAAGGGGG GCCCAGGTCC AGGTCCTGCA GGGCAAGGAG 
CCCCCCTGTT TCCTGCAGTG CTTCCAGGGG GGGATGGTGG TCCACTCCGG GAGACGGGAG 
GAGGAGGAAG AGAACACACA GAGCGAGTGG CGGCTGTACT GTGTGCGCGG AGAGGTGCCT 
GTGGAGGGGA ACTTGCTGGA GGTGGCCTGT CACTGCAGCA GCCTCAGGTC TAGGACGTCC 
ATGGTTGTCC TCAACGTCCA CAAAGCCCTC ATCTACCTGT GGCATGGATG CAAAGCCCAG 
GCCCACACGA AGGAGGTCGG ACGGACTGCA GCCAATAAAA TCAAGGACCA ATGTCCCCTG 
GAGGCCGGGC TGCACAGTAG CAGCAAAGTG ACAATTCATG AGTGTGATGA AGGCTCGGAG 
CCCCTGGGGT TCTGGGACGC TCTGGGGAGG AGAGACCGGA AAGCCTACGA CTGCATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCC CGCCTGTTCA TCCTCAGCAG CTCCTCTGGT 
GACTTCTCAG CCACAGAGTT CATGTATCCC GCCCGAGACC CCTCTGTGGT CAACTCTATG 
CCCTTCCTGC AGGAAGACCT ATACAGCGCC CCACAGCCAG CACTCTTCCT TGTTGACAAT 
CACCACGAGG TGTACCTCTG GCAAGGCTGG TGGCCCATCG AGAACAAGAT CACGGGCTCC 
GCGCGCATCC GCTGGGCCTC GGACCGGAAG AGCGCCATGG AGACAGTGCT GCAGTACTGC 
CGAGGGAAAA ACCTGAAGAA GCCGCCCCCC AAGTCTTACC TCATCCACGC TGGCCTGGAA 
CCCCTGACCT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAAGACAT TGCAGAGATC 
ACGGAGATGG ACACGGAAGT TTCAAATCAG ATCACCCTAG TGGAGGATGT CTTAGCCAAA 
CTGTGTAAGA CCATTTACCC ACTGGCCGAC CTCCTAGCCA GGCCGCTCCC TGAGGGAGTT 
GACCCTCTGA AGCTGGAGAT CTATCTCACC GACGAGGACT TCGAGTTTGC ACTAGACATG 
ACCCGAGACG AGTACAATGC ACTGCCCGCT TGGAAGCAGG TGAACCTGAA GAAAGCGAAA 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856050.1
CDS	748..7299
Translation	MKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASIGKPKHEWFLQKDSEGDTPSLINWPSRVKVREKLVREESARSSPELTSESLTQRRHQTAPGHYLAFQSENSAFDRVSGKVASSARQPIRGYVQPAEPVHTITLVTSDTPESISEGSWVGPAPQTVTKPPPSKVLEGERRDTPVLHICESKAEDVLFSDALEKTRKTLAVLEDRGSGRSQEAPSGTEDLSQPAVGIVTAEPQKESESLAHPPMAQQQPTERMGRSEMVMYVQSEAVSQGHRKEVPTRKHRVLTRSLSDYTGPPQLQALKAKAPAPKRDAESQTSKAELELGLLDTKVSVAQLRNAFLESARASRKPELSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000282.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000282.1

ORF Insert Sequence:

ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC TTAGAAGGAA TTGAAACCGA CACGCAGCCC 
ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG ACTCATCGCC TGCTGGAGGA GGACACACCC 
AGATACATGC GTGCCACAGA CCCGGCCAGC CCGCACATTG GTCGATCAAA TGAAGAAGAA 
GAAACTTCAG ATTCTTCACT AGAAAAGCAG ACTCGATCCA AACAGTGCAC AGAAACCTCA 
GGCATCCATG CTGACTCGCC CTACAGTTCA GGCATCATGG ACACCCAGAG CCTCGAGTCC 
AAAGCCGAAA GAATCGCCAG GTACAAAGCA GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT 
GGGCTCACCC TGGATCCGGA AGCAGACTCT GAAACTCCAT CTCGATACAG CAGGTCCAGA 
AAGGACCCCG AGGCTGCGGA GAAAAGGGGA GTAAGAAGCG AGCGATCGGC CGAGTCCAGC 
AGAGACGCCG GCTCATCCTA CTCCAGGACT GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT 
GAGTCCAAGG ACTATGGCCT GCACCGGAGT GACGGTGTTT CCGACACAGA GGTGCTGCTC 
AATGCAGAAA ACCAAAGACG CGGTCAAGAG CCCAGTGCCA CCGGGCTGGC CCGAGACCTG 
CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC TCATTCTCCG GGCGAGACTC TGCCCTCGGT 
GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC AGCCTGCCCA GTCCATCGCC TGGGCAGCCG 
GCCTCCCCAA GCCACTCCAC CAGTGACCTG CCACTCCCTG CGGAGGCTCG AGCCAGTATC 
GGGAAACCCA AACATGAGTG GTTTCTCCAG AAAGATTCCG AAGGGGACAC ACCTTCACTT 
ATCAACTGGC CATCCAGAGT TAAAGTTAGA GAAAAATTGG TGCGAGAGGA GAGCGCTCGC 
AGCAGCCCTG AACTTACCTC CGAGTCCTTA ACTCAGAGGA GACACCAGAC GGCACCAGGC 
CATTACCTGG CCTTTCAGTC AGAGAACTCG GCCTTCGATA GGGTCTCGGG CAAGGTGGCC 
AGCTCCGCAA GACAGCCCAT CCGCGGCTAC GTCCAGCCAG CAGAGCCTGT CCACACCATC 
ACGCTGGTGA CCTCAGACAC CCCAGAAAGC ATCTCCGAGG GCAGCTGGGT GGGTCCAGCC 
CCCCAGACTG TCACAAAGCC TCCCCCCTCA AAGGTTCTAG AAGGTGAAAG AAGAGACACC 
CCGGTTCTCC ATATTTGCGA ATCCAAAGCA GAAGATGTGC TCTTCTCTGA TGCTCTGGAG 
AAAACCAGGA AGACCCTTGC AGTTTTGGAG GATCGTGGGT CTGGGAGAAG CCAGGAAGCC 
CCCTCTGGAA CTGAGGACTT GAGTCAGCCC GCTGTTGGCA TAGTTACCGC GGAACCTCAG 
AAGGAATCAG AAAGCCTGGC CCACCCGCCT ATGGCTCAGC AGCAGCCCAC TGAGAGGATG 
GGCAGGAGTG AGATGGTCAT GTATGTTCAG AGCGAGGCTG TGTCCCAAGG TCACAGGAAG 
GAGGTGCCCA CGAGGAAACA CAGGGTCCTC ACCCGCTCCC TGTCCGACTA CACGGGCCCT 
CCACAGCTCC AGGCTCTGAA GGCCAAGGCC CCGGCTCCCA AGCGAGATGC CGAGTCACAG 
ACGTCCAAGG CCGAGTTGGA ACTGGGCCTG CTGGACACGA AGGTCTCGGT CGCCCAGCTC 
CGCAATGCGT TCCTGGAGTC GGCCAGGGCC AGCAGGAAAC CCGAGCTGTC AACTTCAAAT 
TCAGAAATGC CAGCTGCCGA GGATGAAGAA AAGGTCGATG AGCGAGCGAG GCTGAGCGTC 
GCTGCTAAGA GGCTGCTCTT CAGGGAGATG GAGAAGTCGT TTGACGAGAA GAGCGTCCCA 
AAGCGCCGCT CCCGCAATGC GGCCGTGGAG CAGAGGCTGC GGCGCCTCCA GGACCGGTCC 
CACACGCAGC CCGTCACCAC CGAGGAGGTG GTCATTGCAG CCACTGAACC TACCCCCGCT 
TCGTGCTCAG TGGCCACCCA CCCTGTAATG ACAAGACATC CTAGCCCCAC TGTAGCTAAG 
AGTCCCGTGC AGCCTGCCAG ATTGCAGGCG TCTGCTCACC AAAAGGCACT AGCCAGAGAC 
CAGACAAACG AGAGCAAAGA TTCTGCTGAG CAGGGAGAAC CTGACTCCTC CACTCTGAGC 
TTAGCGGAGA AGCTGGCCTT GTTTAACAAA CTATCTCAGC CGGTCTCAAA AGCCATTTCA 
ACACGGAACA GATTAGACAT GAGACAGAGG AGGATGAATG CTCGTTATCA AACCCAGCCG 
GTCACGCTTG GAGAAGTGGA ACAGGTGCAG AGTGGCAAGC TCATGGCTTT CTCTCCCACC 
ATTAACACTT CCGTGTCCAC CGTGGCATCT ACCGTCCCCC CCATGTATGC AGGAAATCTT 
CGGACAAAGC CACTTCCAGA TGACAGCTTT GGTGCCACTG AGCAGAAGTT TGCTTCTTCG 
CTAGAAAACT CCGACTCCCC AGTTAGAAGC ATCCTGAAAT CCCAAGGCTG GCAGCCCTCG 
GTCGAGGGTG CTGGGAGCAA AGCAATGTTG AGAGAATTTG AAGAGACAGA ACGCAAGGGA 
GGCTTGACAG GTGGAGATGG CGGGGTTACA AAGTATGGGT CCTTCGAGGA AGCAGAGCTG 
TCCTACCCCG TCCTCAGCAG AGTCCGGGAG GGAGACAACC ATAAAGAGGC CATCTATGCC 
CTTCCGAGGA AAGGAAGCCT GGAGCTCGCC CATCCTCCCA TCGCCCAGCT TGGTGATGAC 
CTGAAGGAAT TTTCCACCCC AAAGAGCACC ATGCAGGCAA GCCCAGACTG GAAGGAGAGG 
CAGCTCTTTG AAGAGAAGGT GGACTTGGAG AATGTCACAA AGAGGAAGTT TTCGCTGAAA 
GCCGCGGAGT TCGGGGAACC CACTTCTGAG CAGACCGGCG CGGCTGCTGG GAAACCGGCT 
GCTCCGACTG CAACCCCGGT GTCCTGGAAG CCGCAGGATC CCTCCGAACA GCCTCAGGAG 
AAGCGGTATC AGAGCCCGTG TGCGATGTTT GCTGCTGGAG AGATCAAAGC CCCGGCGGTG 
GAGGGCAGCC TCGACTCCCC CAGCAAAACC ATGTCCATTA AAGAAAGATT AGCACTGTTG 
AAGAAGAGCG GTGAGGAAGA CTGGAGAAAC AGACTCAACA GAAAGCAGGA ATACGGCAAA 
GCCTCCATCA CCAGCAGCCT GCACATCCAA GAGACGGAGC AGTCACTCAA GAAGAAGGAA 
GAAGGAGGAT TTACAGATGA TAATGACATT TCTAATCTGC TTTGGGAACC TGTATACGCT 
TCTACTTATT CTCCTGCTCT ACCTGCTGCC CATAAATACC TGTCTTTTGT ATCTATTAAT 
CAGCGGGTCA CAGAAAGTCG AGAGAGCCAG ATGACTATTG AAGAGAGAAA GCATCTCATC 
ACCGTGAGAG AGGACGCCTG GAAGACCAGG GGCAAAGGAG CAGCCAACGA CTCTACCCAG 
TTTACTGTGG CCGGCAGGAT GGTGAAGCGA GGTCTGGCGT CCCCCACTGC CATCACCCCG 
GTAGCATCCC CTGTTTCCAG CAAAGCAAGG GGCACTACAC CAGTTTCCAG ACCCCTGGAA 
GATATCGAAG CCAGACCAGA CATGCAGTTA GAGTCGGACC TCAAGCTGGA CAGGCTGGAA 
ACCTTCCTAA GAAGGCTGAA TAACAAAGTT GGTGGGATGC AAGAAACAGT CCTCACTGTC 
ACTGGGAAAT CGGTGAAAGA GGTGATGAAG CCGGACGATG ATGAAACCTT TGCCAAGTTT 
TACCGCAGCG TGGACTCCTC TCTACCGAGA AGCCCCGTAG AGCTGGACGA GGATTTCGAT 
GTCATTTTTG ATCCTTATGC CCCCAGGTTG ACATCCTCGG TGGCTGAGCA CAAGCGTGCC 
GTGCGGCCCA AGCGCCGGGT CCAGGCTTCC AAAAACCCCC TGAAGATGCT GGCCGCGAGG 
GAAGATCTCC TGCAGGAGTA CACTGAGCAG AGACTGAACG TGGCCTTCGT GGAGTCAAAG 
CGGATGAAAG TCGAAAAGTT GTCCGCCAAC TCCAGCTTCT CAGAAGTCAC CCTGGCGGGG 
TTAGCCAGCA AAGAAAACTT CAGCAACGTC AGCCTGCGGA GCGTCAACCT GACGGAACAG 
AATTCCAACA ACAGTGCAGT GCCCTACAAG AAGCTGATGC TGCTGCAGGT TAAAGGAAGA 
AGACACGTGC AGACGCGGCT GGTGGAGCCT CGCGCCCCCT CCCTCAACAG CGGGGACTGC 
TTCCTCCTGC TCTCGCCCCA TCACTGCTTC CTGTGGGTCG GAGAGTTCGC CAACGTGATC 
GAGAAGGCGA AGGCCTCAGA ACTTGCGAGT TTAATTCAGA CCAAGAGGGA ACTTGGTTGT 
AGAGCAACTT ACATCCAGAC TGTTGAAGAA GGAATTAATA CACACACACA TGCAGCCAAA 
GACTTCTGGA AGCTTCTGGG CGGCCAAGCC AGTTACCAAT CCGCCGGAGA CCCAAAGGAG 
GATGAGCTCT ATGAAACGGC CATAATAGAG ACCAACTGCA TTTACCGTCT GATGGATGAC 
AAACTCGTTC CTGATGATGA CTACTGGGGG AAGATCCCAA AGTGCTCCCT CCTGCAATCA 
AAAGAGGTAC TGGTGTTTGA TTTTGGGAGT GAAGTTTACG TGTGGCATGG AAAAGAAGTC 
ACATTAGCAC AACGGAAAAT CGCCTTTCAG CTGGCAAAGC ACTTGTGGAA TGGAACCTTT 
GACTACGAGA ATTGTGACAT TAACCCGCTG GATCCTGGGG AATGCAATCC CCTCATTCCC 
AGGAAAGGAC AGGGGCGGCC CGACTGGGCA ATATTTGGGA GACTTACAGA ACACAATGAG 
ACGATCTTGT TCAAAGAGAA ATTCCTTGAT TGGACGGAAC TGAAGAGACC TAACGAGAAG 
AACGCCAGCG AACTCGCGCA GCACAAGGAC GATGCCCGGG CAGAGGTCAA GCCTTACGAC 
GTGACGCGGA TGGTGCCCGT GCCCCAGACA ACAGCCGGCA CCGTGCTGGA TGGGGTCAAC 
GTGGGCCGAG GCTACGGGCT GGTGGAAGGG GACGACCGGA GGCAGTTCGA GATCGCCAGC 
ATCTCAGTGG ACGTCTGGCA CATCCTGGAG TTCGACTACA GCAGGCTCCC CAAGCAGAGC 
ATCGGGCAGT TCCACGAAGG CGATGCCTAC GTGGTGAAGT GGAAGTTCAT CGTGAGCACT 
GCAGTGGGAA GCCGGCAGAA GGGGGAGCAC TCGGTCAGGG TGGCTGGCAA AGAGAAATGT 
GTCTACTTCT TCTGGCAAGG CCGCCAGTCG ACCGTGAGTG AGAAGGGCAC ATCAGCTCTG 
ATGACAGTGG AGCTGGACGA GGAAAGGGGG GCCCAGGTCC AGGTCCTGCA GGGCAAGGAG 
CCCCCCTGTT TCCTGCAGTG CTTCCAGGGG GGGATGGTGG TCCACTCCGG GAGACGGGAG 
GAGGAGGAAG AGAACACACA GAGCGAGTGG CGGCTGTACT GTGTGCGCGG AGAGGTGCCT 
GTGGAGGGGA ACTTGCTGGA GGTGGCCTGT CACTGCAGCA GCCTCAGGTC TAGGACGTCC 
ATGGTTGTCC TCAACGTCCA CAAAGCCCTC ATCTACCTGT GGCATGGATG CAAAGCCCAG 
GCCCACACGA AGGAGGTCGG ACGGACTGCA GCCAATAAAA TCAAGGACCA ATGTCCCCTG 
GAGGCCGGGC TGCACAGTAG CAGCAAAGTG ACAATTCATG AGTGTGATGA AGGCTCGGAG 
CCCCTGGGGT TCTGGGACGC TCTGGGGAGG AGAGACCGGA AAGCCTACGA CTGCATGCTT 
CAAGATCCTG GAAATTTTAA CTTCACACCC CGCCTGTTCA TCCTCAGCAG CTCCTCTGGT 
GACTTCTCAG CCACAGAGTT CATGTATCCC GCCCGAGACC CCTCTGTGGT CAACTCTATG 
CCCTTCCTGC AGGAAGACCT ATACAGCGCC CCACAGCCAG CACTCTTCCT TGTTGACAAT 
CACCACGAGG TGTACCTCTG GCAAGGCTGG TGGCCCATCG AGAACAAGAT CACGGGCTCC 
GCGCGCATCC GCTGGGCCTC GGACCGGAAG AGCGCCATGG AGACAGTGCT GCAGTACTGC 
CGAGGGAAAA ACCTGAAGAA GCCGCCCCCC AAGTCTTACC TCATCCACGC TGGCCTGGAA 
CCCCTGACCT TCACCAACAT GTTCCCCAGC TGGGAGCACA GAGAAGACAT TGCAGAGATC 
ACGGAGATGG ACACGGAAGT TTCAAATCAG ATCACCCTAG TGGAGGATGT CTTAGCCAAA 
CTGTGTAAGA CCATTTACCC ACTGGCCGAC CTCCTAGCCA GGCCGCTCCC TGAGGGAGTT 
GACCCTCTGA AGCTGGAGAT CTATCTCACC GACGAGGACT TCGAGTTTGC ACTAGACATG 
ACCCGAGACG AGTACAATGC ACTGCCCGCT TGGAAGCAGG TGAACCTGAA GAAAGCGAAA 
GGTCTGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000284.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856052.1 Bos taurus supervillin (SVIL), transcript variant X22, mRNA.

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CloneID	OBa221416
Clone ID Related Accession (Same CDS sequence)	XM_025000284.1
Accession Version	XM_025000284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856052.1
CDS	356..6463
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASLVLKDSHCVSSLVLKDSHSIGKPKHEWFLQKDSEGDTPSLINWPSSHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000284.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000284.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTCTG GTATTGAAGG ACTCTCACTG TGTTTCCAGT 
CTGGTATTGA AGGACTCTCA CAGTATCGGG AAACCCAAAC ATGAGTGGTT TCTCCAGAAA 
GATTCCGAAG GGGACACACC TTCACTTATC AACTGGCCAT CCAGCCATTC TCGGGTTGAG 
GGGTCAAGCG AAGGCCCTGG TGTGGAGCGG GAGAGAGGGT CCCGGAAACC GAGGCGCTAT 
TTTTCTCCTG GTGAAAATAG AAAGACTTCC GAGAGATTTA GAACTCAACC TATAACCTCG 
GCAGAACGAA AGGAATCGGA TAGGTCAACT TCAAATTCAG AAATGCCAGC TGCCGAGGAT 
GAAGAAAAGG TCGATGAGCG AGCGAGGCTG AGCGTCGCTG CTAAGAGGCT GCTCTTCAGG 
GAGATGGAGA AGTCGTTTGA CGAGAAGAGC GTCCCAAAGC GCCGCTCCCG CAATGCGGCC 
GTGGAGCAGA GGCTGCGGCG CCTCCAGGAC CGGTCCCACA CGCAGCCCGT CACCACCGAG 
GAGGTGGTCA TTGCAGCCAC TGAACCTACC CCCGCTTCGT GCTCAGTGGC CACCCACCCT 
GTAATGACAA GACATCCTAG CCCCACTGTA GCTAAGAGTC CCGTGCAGCC TGCCAGATTG 
CAGGCGTCTG CTCACCAAAA GGCACTAGCC AGAGACCAGA CAAACGAGAG CAAAGATTCT 
GCTGAGCAGG GAGAACCTGA CTCCTCCACT CTGAGCTTAG CGGAGAAGCT GGCCTTGTTT 
AACAAACTAT CTCAGCCGGT CTCAAAAGCC ATTTCAACAC GGAACAGATT AGACATGAGA 
CAGAGGAGGA TGAATGCTCG TTATCAAACC CAGCCGGTCA CGCTTGGAGA AGTGGAACAG 
GTGCAGAGTG GCAAGCTCAT GGCTTTCTCT CCCACCATTA ACACTTCCGT GTCCACCGTG 
GCATCTACCG TCCCCCCCAT GTATGCAGGA AATCTTCGGA CAAAGCCACT TCCAGATGAC 
AGCTTTGGTG CCACTGAGCA GAAGTTTGCT TCTTCGCTAG AAAACTCCGA CTCCCCAGTT 
AGAAGCATCC TGAAATCCCA AGGCTGGCAG CCCTCGGTCG AGGGTGCTGG GAGCAAAGCA 
ATGTTGAGAG AATTTGAAGA GACAGAACGC AAGGGAGGCT TGACAGGTGG AGATGGCGGG 
GTTACAAAGT ATGGGTCCTT CGAGGAAGCA GAGCTGTCCT ACCCCGTCCT CAGCAGAGTC 
CGGGAGGGAG ACAACCATAA AGAGGCCATC TATGCCCTTC CGAGGAAAGG AAGCCTGGAG 
CTCGCCCATC CTCCCATCGC CCAGCTTGGT GATGACCTGA AGGAATTTTC CACCCCAAAG 
AGCACCATGC AGGCAAGCCC AGACTGGAAG GAGAGGCAGC TCTTTGAAGA GAAGGTGGAC 
TTGGAGAATG TCACAAAGAG GAAGTTTTCG CTGAAAGCCG CGGAGTTCGG GGAACCCACT 
TCTGAGCAGA CCGGCGCGGC TGCTGGGAAA CCGGCTGCTC CGACTGCAAC CCCGGTGTCC 
TGGAAGCCGC AGGATCCCTC CGAACAGCCT CAGGAGAAGC GGTATCAGAG CCCGTGTGCG 
ATGTTTGCTG CTGGAGAGAT CAAAGCCCCG GCGGTGGAGG GCAGCCTCGA CTCCCCCAGC 
AAAACCATGT CCATTAAAGA AAGATTAGCA CTGTTGAAGA AGAGCGGTGA GGAAGACTGG 
AGAAACAGAC TCAACAGAAA GCAGGAATAC GGCAAAGCCT CCATCACCAG CAGCCTGCAC 
ATCCAAGAGA CGGAGCAGTC ACTCAAGAAG AAGGAAGAAG GAGGATTTAC AGATGATAAT 
GACATTTCTA ATCTGCTTTG GGAACCTGTA TACGCTTCTA CTTATTCTCC TGCTCTACCT 
GCTGCCCATA AATACCTGTC TTTTGTATCT ATTAATCAGC GGGTCACAGA AAGTCGAGAG 
AGCCAGATGA CTATTGAAGA GAGAAAGCAT CTCATCACCG TGAGAGAGGA CGCCTGGAAG 
ACCAGGGGCA AAGGAGCAGC CAACGACTCT ACCCAGTTTA CTGTGGCCGG CAGGATGGTG 
AAGCGAGGTC TGGCGTCCCC CACTGCCATC ACCCCGGTAG CATCCCCTGT TTCCAGCAAA 
GCAAGGGGCA CTACACCAGT TTCCAGACCC CTGGAAGATA TCGAAGCCAG ACCAGACATG 
CAGTTAGAGT CGGACCTCAA GCTGGACAGG CTGGAAACCT TCCTAAGAAG GCTGAATAAC 
AAAGTTGGTG GGATGCAAGA AACAGTCCTC ACTGTCACTG GGAAATCGGT GAAAGAGGTG 
ATGAAGCCGG ACGATGATGA AACCTTTGCC AAGTTTTACC GCAGCGTGGA CTCCTCTCTA 
CCGAGAAGCC CCGTAGAGCT GGACGAGGAT TTCGATGTCA TTTTTGATCC TTATGCCCCC 
AGGTTGACAT CCTCGGTGGC TGAGCACAAG CGTGCCGTGC GGCCCAAGCG CCGGGTCCAG 
GCTTCCAAAA ACCCCCTGAA GATGCTGGCC GCGAGGGAAG ATCTCCTGCA GGAGTACACT 
GAGCAGAGAC TGAACGTGGC CTTCGTGGAG TCAAAGCGGA TGAAAGTCGA AAAGTTGTCC 
GCCAACTCCA GCTTCTCAGA AGTCACCCTG GCGGGGTTAG CCAGCAAAGA AAACTTCAGC 
AACGTCAGCC TGCGGAGCGT CAACCTGACG GAACAGAATT CCAACAACAG TGCAGTGCCC 
TACAAGAAGC TGATGCTGCT GCAGGTTAAA GGAAGAAGAC ACGTGCAGAC GCGGCTGGTG 
GAGCCTCGCG CCCCCTCCCT CAACAGCGGG GACTGCTTCC TCCTGCTCTC GCCCCATCAC 
TGCTTCCTGT GGGTCGGAGA GTTCGCCAAC GTGATCGAGA AGGCGAAGGC CTCAGAACTT 
GCGAGTTTAA TTCAGACCAA GAGGGAACTT GGTTGTAGAG CAACTTACAT CCAGACTGTT 
GAAGAAGGAA TTAATACACA CACACATGCA GCCAAAGACT TCTGGAAGCT TCTGGGCGGC 
CAAGCCAGTT ACCAATCCGC CGGAGACCCA AAGGAGGATG AGCTCTATGA AACGGCCATA 
ATAGAGACCA ACTGCATTTA CCGTCTGATG GATGACAAAC TCGTTCCTGA TGATGACTAC 
TGGGGGAAGA TCCCAAAGTG CTCCCTCCTG CAATCAAAAG AGGTACTGGT GTTTGATTTT 
GGGAGTGAAG TTTACGTGTG GCATGGAAAA GAAGTCACAT TAGCACAACG GAAAATCGCC 
TTTCAGCTGG CAAAGCACTT GTGGAATGGA ACCTTTGACT ACGAGAATTG TGACATTAAC 
CCGCTGGATC CTGGGGAATG CAATCCCCTC ATTCCCAGGA AAGGACAGGG GCGGCCCGAC 
TGGGCAATAT TTGGGAGACT TACAGAACAC AATGAGACGA TCTTGTTCAA AGAGAAATTC 
CTTGATTGGA CGGAACTGAA GAGACCTAAC GAGAAGAACG CCAGCGAACT CGCGCAGCAC 
AAGGACGATG CCCGGGCAGA GGTCAAGCCT TACGACGTGA CGCGGATGGT GCCCGTGCCC 
CAGACAACAG CCGGCACCGT GCTGGATGGG GTCAACGTGG GCCGAGGCTA CGGGCTGGTG 
GAAGGGGACG ACCGGAGGCA GTTCGAGATC GCCAGCATCT CAGTGGACGT CTGGCACATC 
CTGGAGTTCG ACTACAGCAG GCTCCCCAAG CAGAGCATCG GGCAGTTCCA CGAAGGCGAT 
GCCTACGTGG TGAAGTGGAA GTTCATCGTG AGCACTGCAG TGGGAAGCCG GCAGAAGGGG 
GAGCACTCGG TCAGGGTGGC TGGCAAAGAG AAATGTGTCT ACTTCTTCTG GCAAGGCCGC 
CAGTCGACCG TGAGTGAGAA GGGCACATCA GCTCTGATGA CAGTGGAGCT GGACGAGGAA 
AGGGGGGCCC AGGTCCAGGT CCTGCAGGGC AAGGAGCCCC CCTGTTTCCT GCAGTGCTTC 
CAGGGGGGGA TGGTGGTCCA CTCCGGGAGA CGGGAGGAGG AGGAAGAGAA CACACAGAGC 
GAGTGGCGGC TGTACTGTGT GCGCGGAGAG GTGCCTGTGG AGGGGAACTT GCTGGAGGTG 
GCCTGTCACT GCAGCAGCCT CAGGTCTAGG ACGTCCATGG TTGTCCTCAA CGTCCACAAA 
GCCCTCATCT ACCTGTGGCA TGGATGCAAA GCCCAGGCCC ACACGAAGGA GGTCGGACGG 
ACTGCAGCCA ATAAAATCAA GGACCAATGT CCCCTGGAGG CCGGGCTGCA CAGTAGCAGC 
AAAGTGACAA TTCATGAGTG TGATGAAGGC TCGGAGCCCC TGGGGTTCTG GGACGCTCTG 
GGGAGGAGAG ACCGGAAAGC CTACGACTGC ATGCTTCAAG ATCCTGGAAA TTTTAACTTC 
ACACCCCGCC TGTTCATCCT CAGCAGCTCC TCTGGTGACT TCTCAGCCAC AGAGTTCATG 
TATCCCGCCC GAGACCCCTC TGTGGTCAAC TCTATGCCCT TCCTGCAGGA AGACCTATAC 
AGCGCCCCAC AGCCAGCACT CTTCCTTGTT GACAATCACC ACGAGGTGTA CCTCTGGCAA 
GGCTGGTGGC CCATCGAGAA CAAGATCACG GGCTCCGCGC GCATCCGCTG GGCCTCGGAC 
CGGAAGAGCG CCATGGAGAC AGTGCTGCAG TACTGCCGAG GGAAAAACCT GAAGAAGCCG 
CCCCCCAAGT CTTACCTCAT CCACGCTGGC CTGGAACCCC TGACCTTCAC CAACATGTTC 
CCCAGCTGGG AGCACAGAGA AGACATTGCA GAGATCACGG AGATGGACAC GGAAGTTTCA 
AATCAGATCA CCCTAGTGGA GGATGTCTTA GCCAAACTGT GTAAGACCAT TTACCCACTG 
GCCGACCTCC TAGCCAGGCC GCTCCCTGAG GGAGTTGACC CTCTGAAGCT GGAGATCTAT 
CTCACCGACG AGGACTTCGA GTTTGCACTA GACATGACCC GAGACGAGTA CAATGCACTG 
CCCGCTTGGA AGCAGGTGAA CCTGAAGAAA GCGAAAGGTC TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SVIL ( XM_025000285.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856053.1 Bos taurus supervillin (SVIL), transcript variant X23, mRNA.

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CloneID	OBa221417
Clone ID Related Accession (Same CDS sequence)	XM_025000285.1
Accession Version	XM_025000285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	supervillin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGCCA TTCTCGGGTT 
GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC 
TATTTTTCTC CTGGTGAAAA TAGAAAGACT TCCGAGAGAT TTAGAACTCA ACCTATAACC 
TCGGCAGAAC GAAAGGAATC GGATAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACTGAACCT ACCCCCGCTT CGTGCTCAGT GGCCACCCAC 
CCTGTAATGA CAAGACATCC TAGCCCCACT GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA 
TTGCAGGCGT CTGCTCACCA AAAGGCACTA GCCAGAGACC AGACAAACGA GAGCAAAGAT 
TCTGCTGAGC AGGGAGAACC TGACTCCTCC ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG 
TTTAACAAAC TATCTCAGCC GGTCTCAAAA GCCATTTCAA CACGGAACAG ATTAGACATG 
AGACAGAGGA GGATGAATGC TCGTTATCAA ACCCAGCCGG TCACGCTTGG AGAAGTGGAA 
CAGGTGCAGA GTGGCAAGCT CATGGCTTTC TCTCCCACCA TTAACACTTC CGTGTCCACC 
GTGGCATCTA CCGTCCCCCC CATGTATGCA GGAAATCTTC GGACAAAGCC ACTTCCAGAT 
GACAGCTTTG GTGCCACTGA GCAGAAGTTT GCTTCTTCGC TAGAAAACTC CGACTCCCCA 
GTTAGAAGCA TCCTGAAATC CCAAGGCTGG CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA 
GCAATGTTGA GAGAATTTGA AGAGACAGAA CGCAAGGGAG GCTTGACAGG TGGAGATGGC 
GGGGTTACAA AGTATGGGTC CTTCGAGGAA GCAGAGCTGT CCTACCCCGT CCTCAGCAGA 
GTCCGGGAGG GAGACAACCA TAAAGAGGCC ATCTATGCCC TTCCGAGGAA AGGAAGCCTG 
GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT GGTGATGACC TGAAGGAATT TTCCACCCCA 
AAGAGCACCA TGCAGGCAAG CCCAGACTGG AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG 
GACTTGGAGA ATGTCACAAA GAGGAAGTTT TCGCTGAAAG CCGCGGAGTT CGGGGAACCC 
ACTTCTGAGC AGACCGGCGC GGCTGCTGGG AAACCGGCTG CTCCGACTGC AACCCCGGTG 
TCCTGGAAGC CGCAGGATCC CTCCGAACAG CCTCAGGAGA AGCGGTATCA GAGCCCGTGT 
GCGATGTTTG CTGCTGGAGA GATCAAAGCC CCGGCGGTGG AGGGCAGCCT CGACTCCCCC 
AGCAAAACCA TGTCCATTAA AGAAAGATTA GCACTGTTGA AGAAGAGCGG TGAGGAAGAC 
TGGAGAAACA GACTCAACAG AAAGCAGGAA TACGGCAAAG CCTCCATCAC CAGCAGCCTG 
CACATCCAAG AGACGGAGCA GTCACTCAAG AAGAAGGAAG AAGGAGGATT TACAGATGAT 
AATGACATTT CTAATCTGCT TTGGGAACCT GTATACGCTT CTACTTATTC TCCTGCTCTA 
CCTGCTGCCC ATAAATACCT GTCTTTTGTA TCTATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAGA TGACTATTGA AGAGAGAAAG CATCTCATCA CCGTGAGAGA GGACGCCTGG 
AAGACCAGGG GCAAAGGAGC AGCCAACGAC TCTACCCAGT TTACTGTGGC CGGCAGGATG 
GTGAAGCGAG GTCTGGCGTC CCCCACTGCC ATCACCCCGG TAGCATCCCC TGTTTCCAGC 
AAAGCAAGGG GCACTACACC AGTTTCCAGA CCCCTGGAAG ATATCGAAGC CAGACCAGAC 
ATGCAGTTAG AGTCGGACCT CAAGCTGGAC AGGCTGGAAA CCTTCCTAAG AAGGCTGAAT 
AACAAAGTTG GTGGGATGCA AGAAACAGTC CTCACTGTCA CTGGGAAATC GGTGAAAGAG 
GTGATGAAGC CGGACGATGA TGAAACCTTT GCCAAGTTTT ACCGCAGCGT GGACTCCTCT 
CTACCGAGAA GCCCCGTAGA GCTGGACGAG GATTTCGATG TCATTTTTGA TCCTTATGCC 
CCCAGGTTGA CATCCTCGGT GGCTGAGCAC AAGCGTGCCG TGCGGCCCAA GCGCCGGGTC 
CAGGCTTCCA AAAACCCCCT GAAGATGCTG GCCGCGAGGG AAGATCTCCT GCAGGAGTAC 
ACTGAGCAGA GACTGAACGT GGCCTTCGTG GAGTCAAAGC GGATGAAAGT CGAAAAGTTG 
TCCGCCAACT CCAGCTTCTC AGAAGTCACC CTGGCGGGGT TAGCCAGCAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ATTCCAACAA CAGTGCAGTG 
CCCTACAAGA AGCTGATGCT GCTGCAGGTT AAAGGAAGAA GACACGTGCA GACGCGGCTG 
GTGGAGCCTC GCGCCCCCTC CCTCAACAGC GGGGACTGCT TCCTCCTGCT CTCGCCCCAT 
CACTGCTTCC TGTGGGTCGG AGAGTTCGCC AACGTGATCG AGAAGGCGAA GGCCTCAGAA 
CTTGCGAGTT TAATTCAGAC CAAGAGGGAA CTTGGTTGTA GAGCAACTTA CATCCAGACT 
GTTGAAGAAG GAATTAATAC ACACACACAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGC 
GGCCAAGCCA GTTACCAATC CGCCGGAGAC CCAAAGGAGG ATGAGCTCTA TGAAACGGCC 
ATAATAGAGA CCAACTGCAT TTACCGTCTG ATGGATGACA AACTCGTTCC TGATGATGAC 
TACTGGGGGA AGATCCCAAA GTGCTCCCTC CTGCAATCAA AAGAGGTACT GGTGTTTGAT 
TTTGGGAGTG AAGTTTACGT GTGGCATGGA AAAGAAGTCA CATTAGCACA ACGGAAAATC 
GCCTTTCAGC TGGCAAAGCA CTTGTGGAAT GGAACCTTTG ACTACGAGAA TTGTGACATT 
AACCCGCTGG ATCCTGGGGA ATGCAATCCC CTCATTCCCA GGAAAGGACA GGGGCGGCCC 
GACTGGGCAA TATTTGGGAG ACTTACAGAA CACAATGAGA CGATCTTGTT CAAAGAGAAA 
TTCCTTGATT GGACGGAACT GAAGAGACCT AACGAGAAGA ACGCCAGCGA ACTCGCGCAG 
CACAAGGACG ATGCCCGGGC AGAGGTCAAG CCTTACGACG TGACGCGGAT GGTGCCCGTG 
CCCCAGACAA CAGCCGGCAC CGTGCTGGAT GGGGTCAACG TGGGCCGAGG CTACGGGCTG 
GTGGAAGGGG ACGACCGGAG GCAGTTCGAG ATCGCCAGCA TCTCAGTGGA CGTCTGGCAC 
ATCCTGGAGT TCGACTACAG CAGGCTCCCC AAGCAGAGCA TCGGGCAGTT CCACGAAGGC 
GATGCCTACG TGGTGAAGTG GAAGTTCATC GTGAGCACTG CAGTGGGAAG CCGGCAGAAG 
GGGGAGCACT CGGTCAGGGT GGCTGGCAAA GAGAAATGTG TCTACTTCTT CTGGCAAGGC 
CGCCAGTCGA CCGTGAGTGA GAAGGGCACA TCAGCTCTGA TGACAGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTCCTGCAG GGCAAGGAGC CCCCCTGTTT CCTGCAGTGC 
TTCCAGGGGG GGATGGTGGT CCACTCCGGG AGACGGGAGG AGGAGGAAGA GAACACACAG 
AGCGAGTGGC GGCTGTACTG TGTGCGCGGA GAGGTGCCTG TGGAGGGGAA CTTGCTGGAG 
GTGGCCTGTC ACTGCAGCAG CCTCAGGTCT AGGACGTCCA TGGTTGTCCT CAACGTCCAC 
AAAGCCCTCA TCTACCTGTG GCATGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
CGGACTGCAG CCAATAAAAT CAAGGACCAA TGTCCCCTGG AGGCCGGGCT GCACAGTAGC 
AGCAAAGTGA CAATTCATGA GTGTGATGAA GGCTCGGAGC CCCTGGGGTT CTGGGACGCT 
CTGGGGAGGA GAGACCGGAA AGCCTACGAC TGCATGCTTC AAGATCCTGG AAATTTTAAC 
TTCACACCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGTG ACTTCTCAGC CACAGAGTTC 
ATGTATCCCG CCCGAGACCC CTCTGTGGTC AACTCTATGC CCTTCCTGCA GGAAGACCTA 
TACAGCGCCC CACAGCCAGC ACTCTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACGGGCTCCG CGCGCATCCG CTGGGCCTCG 
GACCGGAAGA GCGCCATGGA GACAGTGCTG CAGTACTGCC GAGGGAAAAA CCTGAAGAAG 
CCGCCCCCCA AGTCTTACCT CATCCACGCT GGCCTGGAAC CCCTGACCTT CACCAACATG 
TTCCCCAGCT GGGAGCACAG AGAAGACATT GCAGAGATCA CGGAGATGGA CACGGAAGTT 
TCAAATCAGA TCACCCTAGT GGAGGATGTC TTAGCCAAAC TGTGTAAGAC CATTTACCCA 
CTGGCCGACC TCCTAGCCAG GCCGCTCCCT GAGGGAGTTG ACCCTCTGAA GCTGGAGATC 
TATCTCACCG ACGAGGACTT CGAGTTTGCA CTAGACATGA CCCGAGACGA GTACAATGCA 
CTGCCCGCTT GGAAGCAGGT GAACCTGAAG AAAGCGAAAG GTCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856053.1
CDS	356..6406
Translation	MDTYLNKKNLSLVGFPCQRKVPSFQKEFLLEDKEQLDNHKRRIDAQLLAALPKVAELRKIFEPKRKEFLEMKRKERIARRLEGIETDTQPILLQSCTGLVTHRLLEEDTPRYMRATDPASPHIGRSNEEEETSDSSLEKQTRSKQCTETSGIHADSPYSSGIMDTQSLESKAERIARYKAERRRQLAEKYGLTLDPEADSETPSRYSRSRKDPEAAEKRGVRSERSAESSRDAGSSYSRTELSGLRTCVAESKDYGLHRSDGVSDTEVLLNAENQRRGQEPSATGLARDLPLAGEVSSSFSFSGRDSALGEVPRSPKAVHSLPSPSPGQPASPSHSTSDLPLPAEARASIGKPKHEWFLQKDSEGDTPSLINWPSSHSRVEGSSEGPGVERERGSRKPRRYFSPGENRKTSERFRTQPITSAERKESDRSTSNSEMPAAEDEEKVDERARLSVAAKRLLFREMEKSFDEKSVPKRRSRNAAVEQRLRRLQDRSHTQPVTTEEVVIAATEPTPASCSVATHPVMTRHPSPTVAKSPVQPARLQASAHQKALARDQTNESKDSAEQGEPDSSTLSLAEKLALFNKLSQPVSKAISTRNRLDMRQRRMNARYQTQPVTLGEVEQVQSGKLMAFSPTINTSVSTVASTVPPMYAGNLRTKPLPDDSFGATEQKFASSLENSDSPVRSILKSQGWQPSVEGAGSKAMLREFEETERKGGLTGGDGGVTKYGSFEEAELSYPVLSRVREGDNHKEAIYALPRKGSLELAHPPIAQLGDDLKEFSTPKSTMQASPDWKERQLFEEKVDLENVTKRKFSLKAAEFGEPTSEQTGAAAGKPAAPTATPVSWKPQDPSEQPQEKRYQSPCAMFAAGEIKAPAVEGSLDSPSKTMSIKERLALLKKSGEEDWRNRLNRKQEYGKASITSSLHIQETEQSLKKKEEGGFTDDNDISNLLWEPVYASTYSPALPAAHKYLSFVSINQRVTESRESQMTIEERKHLITVREDAWKTRGKGAANDSTQFTVAGRMVKRGLASPTAITPVASPVSSKARGTTPVSRPLEDIEARPDMQLESDLKLDRLETFLRRLNNKVGGMQETVLTVTGKSVKEVMKPDDDETFAKFYRSVDSSLPRSPVELDEDFDVIFDPYAPRLTSSVAEHKRAVRPKRRVQASKNPLKMLAAREDLLQEYTEQRLNVAFVESKRMKVEKLSANSSFSEVTLAGLASKENFSNVSLRSVNLTEQNSNNSAVPYKKLMLLQVKGRRHVQTRLVEPRAPSLNSGDCFLLLSPHHCFLWVGEFANVIEKAKASELASLIQTKRELGCRATYIQTVEEGINTHTHAAKDFWKLLGGQASYQSAGDPKEDELYETAIIETNCIYRLMDDKLVPDDDYWGKIPKCSLLQSKEVLVFDFGSEVYVWHGKEVTLAQRKIAFQLAKHLWNGTFDYENCDINPLDPGECNPLIPRKGQGRPDWAIFGRLTEHNETILFKEKFLDWTELKRPNEKNASELAQHKDDARAEVKPYDVTRMVPVPQTTAGTVLDGVNVGRGYGLVEGDDRRQFEIASISVDVWHILEFDYSRLPKQSIGQFHEGDAYVVKWKFIVSTAVGSRQKGEHSVRVAGKEKCVYFFWQGRQSTVSEKGTSALMTVELDEERGAQVQVLQGKEPPCFLQCFQGGMVVHSGRREEEEENTQSEWRLYCVRGEVPVEGNLLEVACHCSSLRSRTSMVVLNVHKALIYLWHGCKAQAHTKEVGRTAANKIKDQCPLEAGLHSSSKVTIHECDEGSEPLGFWDALGRRDRKAYDCMLQDPGNFNFTPRLFILSSSSGDFSATEFMYPARDPSVVNSMPFLQEDLYSAPQPALFLVDNHHEVYLWQGWWPIENKITGSARIRWASDRKSAMETVLQYCRGKNLKKPPPKSYLIHAGLEPLTFTNMFPSWEHREDIAEITEMDTEVSNQITLVEDVLAKLCKTIYPLADLLARPLPEGVDPLKLEIYLTDEDFEFALDMTRDEYNALPAWKQVNLKKAKGLF

Target ORF information:

RefSeq Version	XM_025000285.1
Organism	Bos taurus(cattle)
Definition	Bos taurus supervillin (SVIL), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025000285.1

ORF Insert Sequence:

ATGGATACCT ATTTGAACAA GAAAAACTTA AGCCTCGTTG GATTCCCGTG TCAAAGGAAA 
GTTCCAAGCT TTCAGAAAGA GTTCTTGCTT GAAGACAAAG AACAGCTTGA TAACCACAAA 
AGAAGGATCG ATGCTCAGCT TTTAGCTGCC CTTCCTAAAG TTGCAGAATT AAGAAAAATC 
TTTGAACCAA AGAGGAAAGA ATTTTTAGAA ATGAAAAGAA AAGAAAGAAT TGCCCGGCGC 
TTAGAAGGAA TTGAAACCGA CACGCAGCCC ATCCTCTTGC AGAGCTGCAC GGGCTTGGTG 
ACTCATCGCC TGCTGGAGGA GGACACACCC AGATACATGC GTGCCACAGA CCCGGCCAGC 
CCGCACATTG GTCGATCAAA TGAAGAAGAA GAAACTTCAG ATTCTTCACT AGAAAAGCAG 
ACTCGATCCA AACAGTGCAC AGAAACCTCA GGCATCCATG CTGACTCGCC CTACAGTTCA 
GGCATCATGG ACACCCAGAG CCTCGAGTCC AAAGCCGAAA GAATCGCCAG GTACAAAGCA 
GAGAGAAGAC GGCAGCTGGC AGAAAAGTAT GGGCTCACCC TGGATCCGGA AGCAGACTCT 
GAAACTCCAT CTCGATACAG CAGGTCCAGA AAGGACCCCG AGGCTGCGGA GAAAAGGGGA 
GTAAGAAGCG AGCGATCGGC CGAGTCCAGC AGAGACGCCG GCTCATCCTA CTCCAGGACT 
GAGCTCTCGG GGCTCAGGAC CTGCGTGGCT GAGTCCAAGG ACTATGGCCT GCACCGGAGT 
GACGGTGTTT CCGACACAGA GGTGCTGCTC AATGCAGAAA ACCAAAGACG CGGTCAAGAG 
CCCAGTGCCA CCGGGCTGGC CCGAGACCTG CCCCTTGCAG GGGAGGTCTC CTCTTCTTTC 
TCATTCTCCG GGCGAGACTC TGCCCTCGGT GAAGTGCCAA GGTCCCCAAA GGCAGTGCAC 
AGCCTGCCCA GTCCATCGCC TGGGCAGCCG GCCTCCCCAA GCCACTCCAC CAGTGACCTG 
CCACTCCCTG CGGAGGCTCG AGCCAGTATC GGGAAACCCA AACATGAGTG GTTTCTCCAG 
AAAGATTCCG AAGGGGACAC ACCTTCACTT ATCAACTGGC CATCCAGCCA TTCTCGGGTT 
GAGGGGTCAA GCGAAGGCCC TGGTGTGGAG CGGGAGAGAG GGTCCCGGAA ACCGAGGCGC 
TATTTTTCTC CTGGTGAAAA TAGAAAGACT TCCGAGAGAT TTAGAACTCA ACCTATAACC 
TCGGCAGAAC GAAAGGAATC GGATAGGTCA ACTTCAAATT CAGAAATGCC AGCTGCCGAG 
GATGAAGAAA AGGTCGATGA GCGAGCGAGG CTGAGCGTCG CTGCTAAGAG GCTGCTCTTC 
AGGGAGATGG AGAAGTCGTT TGACGAGAAG AGCGTCCCAA AGCGCCGCTC CCGCAATGCG 
GCCGTGGAGC AGAGGCTGCG GCGCCTCCAG GACCGGTCCC ACACGCAGCC CGTCACCACC 
GAGGAGGTGG TCATTGCAGC CACTGAACCT ACCCCCGCTT CGTGCTCAGT GGCCACCCAC 
CCTGTAATGA CAAGACATCC TAGCCCCACT GTAGCTAAGA GTCCCGTGCA GCCTGCCAGA 
TTGCAGGCGT CTGCTCACCA AAAGGCACTA GCCAGAGACC AGACAAACGA GAGCAAAGAT 
TCTGCTGAGC AGGGAGAACC TGACTCCTCC ACTCTGAGCT TAGCGGAGAA GCTGGCCTTG 
TTTAACAAAC TATCTCAGCC GGTCTCAAAA GCCATTTCAA CACGGAACAG ATTAGACATG 
AGACAGAGGA GGATGAATGC TCGTTATCAA ACCCAGCCGG TCACGCTTGG AGAAGTGGAA 
CAGGTGCAGA GTGGCAAGCT CATGGCTTTC TCTCCCACCA TTAACACTTC CGTGTCCACC 
GTGGCATCTA CCGTCCCCCC CATGTATGCA GGAAATCTTC GGACAAAGCC ACTTCCAGAT 
GACAGCTTTG GTGCCACTGA GCAGAAGTTT GCTTCTTCGC TAGAAAACTC CGACTCCCCA 
GTTAGAAGCA TCCTGAAATC CCAAGGCTGG CAGCCCTCGG TCGAGGGTGC TGGGAGCAAA 
GCAATGTTGA GAGAATTTGA AGAGACAGAA CGCAAGGGAG GCTTGACAGG TGGAGATGGC 
GGGGTTACAA AGTATGGGTC CTTCGAGGAA GCAGAGCTGT CCTACCCCGT CCTCAGCAGA 
GTCCGGGAGG GAGACAACCA TAAAGAGGCC ATCTATGCCC TTCCGAGGAA AGGAAGCCTG 
GAGCTCGCCC ATCCTCCCAT CGCCCAGCTT GGTGATGACC TGAAGGAATT TTCCACCCCA 
AAGAGCACCA TGCAGGCAAG CCCAGACTGG AAGGAGAGGC AGCTCTTTGA AGAGAAGGTG 
GACTTGGAGA ATGTCACAAA GAGGAAGTTT TCGCTGAAAG CCGCGGAGTT CGGGGAACCC 
ACTTCTGAGC AGACCGGCGC GGCTGCTGGG AAACCGGCTG CTCCGACTGC AACCCCGGTG 
TCCTGGAAGC CGCAGGATCC CTCCGAACAG CCTCAGGAGA AGCGGTATCA GAGCCCGTGT 
GCGATGTTTG CTGCTGGAGA GATCAAAGCC CCGGCGGTGG AGGGCAGCCT CGACTCCCCC 
AGCAAAACCA TGTCCATTAA AGAAAGATTA GCACTGTTGA AGAAGAGCGG TGAGGAAGAC 
TGGAGAAACA GACTCAACAG AAAGCAGGAA TACGGCAAAG CCTCCATCAC CAGCAGCCTG 
CACATCCAAG AGACGGAGCA GTCACTCAAG AAGAAGGAAG AAGGAGGATT TACAGATGAT 
AATGACATTT CTAATCTGCT TTGGGAACCT GTATACGCTT CTACTTATTC TCCTGCTCTA 
CCTGCTGCCC ATAAATACCT GTCTTTTGTA TCTATTAATC AGCGGGTCAC AGAAAGTCGA 
GAGAGCCAGA TGACTATTGA AGAGAGAAAG CATCTCATCA CCGTGAGAGA GGACGCCTGG 
AAGACCAGGG GCAAAGGAGC AGCCAACGAC TCTACCCAGT TTACTGTGGC CGGCAGGATG 
GTGAAGCGAG GTCTGGCGTC CCCCACTGCC ATCACCCCGG TAGCATCCCC TGTTTCCAGC 
AAAGCAAGGG GCACTACACC AGTTTCCAGA CCCCTGGAAG ATATCGAAGC CAGACCAGAC 
ATGCAGTTAG AGTCGGACCT CAAGCTGGAC AGGCTGGAAA CCTTCCTAAG AAGGCTGAAT 
AACAAAGTTG GTGGGATGCA AGAAACAGTC CTCACTGTCA CTGGGAAATC GGTGAAAGAG 
GTGATGAAGC CGGACGATGA TGAAACCTTT GCCAAGTTTT ACCGCAGCGT GGACTCCTCT 
CTACCGAGAA GCCCCGTAGA GCTGGACGAG GATTTCGATG TCATTTTTGA TCCTTATGCC 
CCCAGGTTGA CATCCTCGGT GGCTGAGCAC AAGCGTGCCG TGCGGCCCAA GCGCCGGGTC 
CAGGCTTCCA AAAACCCCCT GAAGATGCTG GCCGCGAGGG AAGATCTCCT GCAGGAGTAC 
ACTGAGCAGA GACTGAACGT GGCCTTCGTG GAGTCAAAGC GGATGAAAGT CGAAAAGTTG 
TCCGCCAACT CCAGCTTCTC AGAAGTCACC CTGGCGGGGT TAGCCAGCAA AGAAAACTTC 
AGCAACGTCA GCCTGCGGAG CGTCAACCTG ACGGAACAGA ATTCCAACAA CAGTGCAGTG 
CCCTACAAGA AGCTGATGCT GCTGCAGGTT AAAGGAAGAA GACACGTGCA GACGCGGCTG 
GTGGAGCCTC GCGCCCCCTC CCTCAACAGC GGGGACTGCT TCCTCCTGCT CTCGCCCCAT 
CACTGCTTCC TGTGGGTCGG AGAGTTCGCC AACGTGATCG AGAAGGCGAA GGCCTCAGAA 
CTTGCGAGTT TAATTCAGAC CAAGAGGGAA CTTGGTTGTA GAGCAACTTA CATCCAGACT 
GTTGAAGAAG GAATTAATAC ACACACACAT GCAGCCAAAG ACTTCTGGAA GCTTCTGGGC 
GGCCAAGCCA GTTACCAATC CGCCGGAGAC CCAAAGGAGG ATGAGCTCTA TGAAACGGCC 
ATAATAGAGA CCAACTGCAT TTACCGTCTG ATGGATGACA AACTCGTTCC TGATGATGAC 
TACTGGGGGA AGATCCCAAA GTGCTCCCTC CTGCAATCAA AAGAGGTACT GGTGTTTGAT 
TTTGGGAGTG AAGTTTACGT GTGGCATGGA AAAGAAGTCA CATTAGCACA ACGGAAAATC 
GCCTTTCAGC TGGCAAAGCA CTTGTGGAAT GGAACCTTTG ACTACGAGAA TTGTGACATT 
AACCCGCTGG ATCCTGGGGA ATGCAATCCC CTCATTCCCA GGAAAGGACA GGGGCGGCCC 
GACTGGGCAA TATTTGGGAG ACTTACAGAA CACAATGAGA CGATCTTGTT CAAAGAGAAA 
TTCCTTGATT GGACGGAACT GAAGAGACCT AACGAGAAGA ACGCCAGCGA ACTCGCGCAG 
CACAAGGACG ATGCCCGGGC AGAGGTCAAG CCTTACGACG TGACGCGGAT GGTGCCCGTG 
CCCCAGACAA CAGCCGGCAC CGTGCTGGAT GGGGTCAACG TGGGCCGAGG CTACGGGCTG 
GTGGAAGGGG ACGACCGGAG GCAGTTCGAG ATCGCCAGCA TCTCAGTGGA CGTCTGGCAC 
ATCCTGGAGT TCGACTACAG CAGGCTCCCC AAGCAGAGCA TCGGGCAGTT CCACGAAGGC 
GATGCCTACG TGGTGAAGTG GAAGTTCATC GTGAGCACTG CAGTGGGAAG CCGGCAGAAG 
GGGGAGCACT CGGTCAGGGT GGCTGGCAAA GAGAAATGTG TCTACTTCTT CTGGCAAGGC 
CGCCAGTCGA CCGTGAGTGA GAAGGGCACA TCAGCTCTGA TGACAGTGGA GCTGGACGAG 
GAAAGGGGGG CCCAGGTCCA GGTCCTGCAG GGCAAGGAGC CCCCCTGTTT CCTGCAGTGC 
TTCCAGGGGG GGATGGTGGT CCACTCCGGG AGACGGGAGG AGGAGGAAGA GAACACACAG 
AGCGAGTGGC GGCTGTACTG TGTGCGCGGA GAGGTGCCTG TGGAGGGGAA CTTGCTGGAG 
GTGGCCTGTC ACTGCAGCAG CCTCAGGTCT AGGACGTCCA TGGTTGTCCT CAACGTCCAC 
AAAGCCCTCA TCTACCTGTG GCATGGATGC AAAGCCCAGG CCCACACGAA GGAGGTCGGA 
CGGACTGCAG CCAATAAAAT CAAGGACCAA TGTCCCCTGG AGGCCGGGCT GCACAGTAGC 
AGCAAAGTGA CAATTCATGA GTGTGATGAA GGCTCGGAGC CCCTGGGGTT CTGGGACGCT 
CTGGGGAGGA GAGACCGGAA AGCCTACGAC TGCATGCTTC AAGATCCTGG AAATTTTAAC 
TTCACACCCC GCCTGTTCAT CCTCAGCAGC TCCTCTGGTG ACTTCTCAGC CACAGAGTTC 
ATGTATCCCG CCCGAGACCC CTCTGTGGTC AACTCTATGC CCTTCCTGCA GGAAGACCTA 
TACAGCGCCC CACAGCCAGC ACTCTTCCTT GTTGACAATC ACCACGAGGT GTACCTCTGG 
CAAGGCTGGT GGCCCATCGA GAACAAGATC ACGGGCTCCG CGCGCATCCG CTGGGCCTCG 
GACCGGAAGA GCGCCATGGA GACAGTGCTG CAGTACTGCC GAGGGAAAAA CCTGAAGAAG 
CCGCCCCCCA AGTCTTACCT CATCCACGCT GGCCTGGAAC CCCTGACCTT CACCAACATG 
TTCCCCAGCT GGGAGCACAG AGAAGACATT GCAGAGATCA CGGAGATGGA CACGGAAGTT 
TCAAATCAGA TCACCCTAGT GGAGGATGTC TTAGCCAAAC TGTGTAAGAC CATTTACCCA 
CTGGCCGACC TCCTAGCCAG GCCGCTCCCT GAGGGAGTTG ACCCTCTGAA GCTGGAGATC 
TATCTCACCG ACGAGGACTT CGAGTTTGCA CTAGACATGA CCCGAGACGA GTACAATGCA 
CTGCCCGCTT GGAAGCAGGT GAACCTGAAG AAAGCGAAAG GTCTGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SVIL
Entrez Gene ID	281509
Full Name	supervillin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	SVIL	NP_776615.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007181	XP_565017.3
Danio rerio (zebrafish)	svilb	XP_003197996.2
Xenopus tropicalis (tropical clawed frog)	svil	NP_001090765.1
Pan troglodytes (chimpanzee)	SVIL	XP_001136112.1
Canis lupus familiaris (dog)	SVIL	XP_005617106.1
Mus musculus (house mouse)	Svil	NP_694793.1
Rattus norvegicus (Norway rat)	Svil	NP_001101886.2
Gallus gallus (chicken)	SVIL	XP_004939254.1
	SVIL	XP_001083894.2
Danio rerio (zebrafish)	svila	NP_001030338.2
Homo sapiens (human)	SVIL	NP_068506.2
Drosophila melanogaster (fruit fly)	CG33232	NP_995968.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SVIL cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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