SSX2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SSX2
Entrez Gene ID	6757
Full Name	SSX family member 2
Synonyms	CT5.2,CT5.2A,HD21,HOM-MEL-40,SSX
General protein information	Preferred Names SSX family member 2 Names protein SSX2 cancer/testis antigen 5.2 cancer/testis antigen family 5, member 2a sarcoma, synovial, X-chromosome-related 2 synovial sarcoma, X breakpoint 2 synovial sarcoma, X breakpoint 2B tumor antigen HOM-MEL-40
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.22
Summary	The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. This gene, and also the SSX1 and SSX4 family members, have been involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are likely responsible for transforming activity. Alternative splicing of this gene results in multiple transcript variants. This gene also has an identical duplicate, GeneID: 727837, located about 45 kb downstream in the opposite orientation on chromosome X. [provided by RefSeq, Jul 2013].
Disorder MIM:	300192
Disorder Html:	?Sarcoma, synovial, 300813 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003147.5	NP_003138.3	protein SSX2 isoform a
NM_175698.2	NP_783629.1	protein SSX2 isoform b
NM_001278697.1	NP_001265626.1	protein SSX2 isoform c

Pathways from BioSystems

Homologies

Homo sapiens (human)	SSX2B	NP_001157889.1
Homo sapiens (human)	SSX2	NP_783629.1
Pan troglodytes (chimpanzee)	LOC100609340	XP_003317517.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The impact of chromosomal translocation locus and fusion oncogene coding sequence in synovial sarcomagenesis.
Jones KB, Barrott JJ, Xie M, Haldar M, Jin H, Zhu JF, Monument MJ, Mosbruger TL, Langer EM, Randall RL, Wilson RK, Cairns BR, Ding L, Capecchi MR
Oncogene35(38)5021-32(2016 Sep)

SSX2 regulates focal adhesion but does not drive the epithelial to mesenchymal transition in prostate cancer.
Bloom JE, McNeel DG
Oncotarget7(32)50997-51011(2016 Aug)

The SSX gene family: characterization of 9 complete genes.
G?re AO, Wei IJ, Old LJ, Chen YT
International journal of cancer101(5)448-53(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Meta-analysis of human synovial sarcoma patient series identified two tumor-gentoype-phenotype correlations that were not modeled by the mice, namely a scarcity of male hosts and biphasic histologic features among SS18-SSX2 tumors. Re-analysis of human SS18-SSX1 and SS18-SSX2 tumor transcriptomes demonstrated very few consistent differences, but highlighted increased native SSX2 expression in SS18-SSX1 tumors.
Title: The impact of chromosomal translocation locus and fusion oncogene coding sequence in synovial sarcomagenesis.

results reveal two important phenotypes of ectopic SSX2 expression that may drive/support tumorigenesis: First, immediate induction of genomic instability, and second, long-term support of tumor cell growth.
Title: Ectopic expression of cancer/testis antigen SSX2 induces DNA damage and promotes genomic instability.

primary autoantibodies against intracellular MM-specific tumor antigens SSX-2 and NY-ESO-1 are rare but functional in multiple myeloma patients after allogeneic stem cell transplantation
Title: Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation.

SSX2, SSX3, and SSX4 are expressed in the lung tissue of only 3.5% early-stage non-small cell lung cancer patients.
Title: SSX2-4 expression in early-stage non-small cell lung cancer.

The tumor-specific pattern of expression of SSX2.
Title: Development of a T cell receptor targeting an HLA-A*0201 restricted epitope from the cancer-testis antigen SSX2 for adoptive immunotherapy of cancer.

The following SSX2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSX2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03243	NM_003147.5 Latest version!	Homo sapiens SSX family member 2 (SSX2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu03537	NM_175698.2 Latest version!	Homo sapiens SSX family member 2 (SSX2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04695	NM_001278697.1 Latest version!	Homo sapiens SSX family member 2 (SSX2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSX2 ( NM_003147.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003138.3 Homo sapiens SSX family member 2 (SSX2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03243
Clone ID Related Accession (Same CDS sequence)	NM_003147.5
Accession Version	NM_003147.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	protein SSX2 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC103863.2 and BC002818.2. On Jul 10, 2013 this sequence version replaced NM_003147.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGAACGGAG ACGACGCCTT TGCAAGGAGA CCCACGGTTG GTGCTCAAAT ACCAGAGAAG 
ATCCAAAAGG CCTTCGATGA TATTGCCAAA TACTTCTCTA AGGAAGAGTG GGAAAAGATG 
AAAGCCTCGG AGAAAATCTT CTATGTGTAT ATGAAGAGAA AGTATGAGGC TATGACTAAA 
CTAGGTTTCA AGGCCACCCT CCCACCTTTC ATGTGTAATA AACGGGCCGA AGACTTCCAG 
GGGAATGATT TGGATAATGA CCCTAACCGT GGGAATCAGG TTGAACGTCC TCAGATGACT 
TTCGGCAGGC TCCAGGGAAT CTCCCCGAAG ATCATGCCCA AGAAGCCAGC AGAGGAAGGA 
AATGATTCGG AGGAAGTGCC AGAAGCATCT GGCCCACAAA ATGATGGGAA AGAGCTGTGC 
CCCCCGGGAA AACCAACTAC CTCTGAGAAG ATTCACGAGA GATCTGGAAA TAGGGAGGCC 
CAAGAAAAGG AAGAGAGACG CGGAACAGCT CATCGGTGGA GCAGTCAGAA CACACACAAC 
ATTGGTCGAT TCAGTTTGTC AACTTCTATG GGTGCAGTTC ATGGTACCCC CAAAACAATT 
ACACACAACA GGGACCCAAA AGGGGGGAAC ATGCCTGGAC CCACAGACTG CGTGAGAGAA 
AACAGCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003138.3
CDS	137..808
Translation	MNGDDAFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKASEKIFYVYMKRKYEAMTKLGFKATLPPFMCNKRAEDFQGNDLDNDPNRGNQVERPQMTFGRLQGISPKIMPKKPAEEGNDSEEVPEASGPQNDGKELCPPGKPTTSEKIHERSGNREAQEKEERRGTAHRWSSQNTHNIGRFSLSTSMGAVHGTPKTITHNRDPKGGNMPGPTDCVRENSW

Target ORF information:

RefSeq Version	NM_003147.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SSX family member 2 (SSX2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003147.5

ORF Insert Sequence:

ATGAACGGAG ACGACGCCTT TGCAAGGAGA CCCACGGTTG GTGCTCAAAT ACCAGAGAAG 
ATCCAAAAGG CCTTCGATGA TATTGCCAAA TACTTCTCTA AGGAAGAGTG GGAAAAGATG 
AAAGCCTCGG AGAAAATCTT CTATGTGTAT ATGAAGAGAA AGTATGAGGC TATGACTAAA 
CTAGGTTTCA AGGCCACCCT CCCACCTTTC ATGTGTAATA AACGGGCCGA AGACTTCCAG 
GGGAATGATT TGGATAATGA CCCTAACCGT GGGAATCAGG TTGAACGTCC TCAGATGACT 
TTCGGCAGGC TCCAGGGAAT CTCCCCGAAG ATCATGCCCA AGAAGCCAGC AGAGGAAGGA 
AATGATTCGG AGGAAGTGCC AGAAGCATCT GGCCCACAAA ATGATGGGAA AGAGCTGTGC 
CCCCCGGGAA AACCAACTAC CTCTGAGAAG ATTCACGAGA GATCTGGAAA TAGGGAGGCC 
CAAGAAAAGG AAGAGAGACG CGGAACAGCT CATCGGTGGA GCAGTCAGAA CACACACAAC 
ATTGGTCGAT TCAGTTTGTC AACTTCTATG GGTGCAGTTC ATGGTACCCC CAAAACAATT 
ACACACAACA GGGACCCAAA AGGGGGGAAC ATGCCTGGAC CCACAGACTG CGTGAGAGAA 
AACAGCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSX2 ( NM_175698.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_783629.1 Homo sapiens SSX family member 2 (SSX2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03537
Clone ID Related Accession (Same CDS sequence)	NM_175698.2
Accession Version	NM_175698.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	protein SSX2 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC103863.2, BI560469.1 and BC002818.2. On Jul 10, 2013 this sequence version replaced NM_175698.1. Transcript Variant: This variant (2) lacks an alternate exon which results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) contains a shorter and distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACGGAG ACGACGCCTT TGCAAGGAGA CCCACGGTTG GTGCTCAAAT ACCAGAGAAG 
ATCCAAAAGG CCTTCGATGA TATTGCCAAA TACTTCTCTA AGGAAGAGTG GGAAAAGATG 
AAAGCCTCGG AGAAAATCTT CTATGTGTAT ATGAAGAGAA AGTATGAGGC TATGACTAAA 
CTAGGTTTCA AGGCCACCCT CCCACCTTTC ATGTGTAATA AACGGGCCGA AGACTTCCAG 
GGGAATGATT TGGATAATGA CCCTAACCGT GGGAATCAGG TTGAACGTCC TCAGATGACT 
TTCGGCAGGC TCCAGGGAAT CTCCCCGAAG ATCATGCCCA AGAAGCCAGC AGAGGAAGGA 
AATGATTCGG AGGAAGTGCC AGAAGCATCT GGCCCACAAA ATGATGGGAA AGAGCTGTGC 
CCCCCGGGAA AACCAACTAC CTCTGAGAAG ATTCACGAGA GATCTGGACC CAAAAGGGGG 
GAACATGCCT GGACCCACAG ACTGCGTGAG AGAAAACAGC TGGTGATTTA TGAAGAGATC 
AGCGACCCTG AGGAAGATGA CGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783629.1
CDS	137..703
Translation	MNGDDAFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKASEKIFYVYMKRKYEAMTKLGFKATLPPFMCNKRAEDFQGNDLDNDPNRGNQVERPQMTFGRLQGISPKIMPKKPAEEGNDSEEVPEASGPQNDGKELCPPGKPTTSEKIHERSGPKRGEHAWTHRLRERKQLVIYEEISDPEEDDE

Target ORF information:

RefSeq Version	NM_175698.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SSX family member 2 (SSX2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175698.2

ORF Insert Sequence:

ATGAACGGAG ACGACGCCTT TGCAAGGAGA CCCACGGTTG GTGCTCAAAT ACCAGAGAAG 
ATCCAAAAGG CCTTCGATGA TATTGCCAAA TACTTCTCTA AGGAAGAGTG GGAAAAGATG 
AAAGCCTCGG AGAAAATCTT CTATGTGTAT ATGAAGAGAA AGTATGAGGC TATGACTAAA 
CTAGGTTTCA AGGCCACCCT CCCACCTTTC ATGTGTAATA AACGGGCCGA AGACTTCCAG 
GGGAATGATT TGGATAATGA CCCTAACCGT GGGAATCAGG TTGAACGTCC TCAGATGACT 
TTCGGCAGGC TCCAGGGAAT CTCCCCGAAG ATCATGCCCA AGAAGCCAGC AGAGGAAGGA 
AATGATTCGG AGGAAGTGCC AGAAGCATCT GGCCCACAAA ATGATGGGAA AGAGCTGTGC 
CCCCCGGGAA AACCAACTAC CTCTGAGAAG ATTCACGAGA GATCTGGACC CAAAAGGGGG 
GAACATGCCT GGACCCACAG ACTGCGTGAG AGAAAACAGC TGGTGATTTA TGAAGAGATC 
AGCGACCCTG AGGAAGATGA CGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SSX2 ( NM_001278697.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265626.1 Homo sapiens SSX family member 2 (SSX2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu04695
Clone ID Related Accession (Same CDS sequence)	NM_001278697.1
Accession Version	NM_001278697.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	protein SSX2 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC103863.2, JX014236.1 and BC002818.2. Transcript Variant: This variant (3) uses an alternate splice site that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (c) contains a shorter and distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGAACGGAG ACGACGCCTT TGCAAGGAGA CCCACGGTTG GTGCTCAAAT ACCAGAGAAG 
ATCCAAAAGG CCTTCGATGA TATTGCCAAA TACTTCTCTA AGGAAGAGTG GGAAAAGATG 
AAAGCCTCGG AGAAAATCTT CTATGTGTAT ATGAAGAGAA AGTATGAGGC TATGACTAAA 
CTAGGTTTCA AGGCCACCCT CCCACCTTTC ATGTGTAATA AACGGGCCGA AGACTTCCAG 
GGGAATGATT TGGATAATGA CCCTAACCGT GGGAATCAGG TTGAACGTCC TCAGATGACT 
TTCGGCAGGC TCCAGGGAAT CTCCCCGAAG ATCATGCCCA AGAAGCCAGC AGAGGAAGGA 
AATGATTCGG AGGAAGTGCC AGAAGCATCT GGCCCACAAA ATGATGGGAA AGAGCTGTGC 
CCCCCGGGAA AACCAACTAC CTCTGAGAAG ATTCACGAGA GATCTGGAAA TAGGGAGGCC 
CAAGAAAAGG AAGAGAGACG CGGAACAGCT CATCGGTGGA GCAGTCAGAA CACACACAAC 
ATTGGACCCA AAAGGGGGGA ACATGCCTGG ACCCACAGAC TGCGTGAGAG AAAACAGCTG 
GTGATTTATG AAGAGATCAG CGACCCTGAG GAAGATGACG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265626.1
CDS	137..781
Translation	MNGDDAFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKASEKIFYVYMKRKYEAMTKLGFKATLPPFMCNKRAEDFQGNDLDNDPNRGNQVERPQMTFGRLQGISPKIMPKKPAEEGNDSEEVPEASGPQNDGKELCPPGKPTTSEKIHERSGNREAQEKEERRGTAHRWSSQNTHNIGPKRGEHAWTHRLRERKQLVIYEEISDPEEDDE

Target ORF information:

RefSeq Version	NM_001278697.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SSX family member 2 (SSX2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278697.1

ORF Insert Sequence:

ATGAACGGAG ACGACGCCTT TGCAAGGAGA CCCACGGTTG GTGCTCAAAT ACCAGAGAAG 
ATCCAAAAGG CCTTCGATGA TATTGCCAAA TACTTCTCTA AGGAAGAGTG GGAAAAGATG 
AAAGCCTCGG AGAAAATCTT CTATGTGTAT ATGAAGAGAA AGTATGAGGC TATGACTAAA 
CTAGGTTTCA AGGCCACCCT CCCACCTTTC ATGTGTAATA AACGGGCCGA AGACTTCCAG 
GGGAATGATT TGGATAATGA CCCTAACCGT GGGAATCAGG TTGAACGTCC TCAGATGACT 
TTCGGCAGGC TCCAGGGAAT CTCCCCGAAG ATCATGCCCA AGAAGCCAGC AGAGGAAGGA 
AATGATTCGG AGGAAGTGCC AGAAGCATCT GGCCCACAAA ATGATGGGAA AGAGCTGTGC 
CCCCCGGGAA AACCAACTAC CTCTGAGAAG ATTCACGAGA GATCTGGAAA TAGGGAGGCC 
CAAGAAAAGG AAGAGAGACG CGGAACAGCT CATCGGTGGA GCAGTCAGAA CACACACAAC 
ATTGGACCCA AAAGGGGGGA ACATGCCTGG ACCCACAGAC TGCGTGAGAG AAAACAGCTG 
GTGATTTATG AAGAGATCAG CGACCCTGAG GAAGATGACG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The impact of chromosomal translocation locus and fusion oncogene coding sequence in synovial sarcomagenesis.
Oncogene35(38)5021-32(2016 Sep)
Jones KB,Barrott JJ,Xie M,Haldar M,Jin H,Zhu JF,Monument MJ,Mosbruger TL,Langer EM,Randall RL,Wilson RK,Cairns BR,Ding L,Capecchi MR

SSX2 regulates focal adhesion but does not drive the epithelial to mesenchymal transition in prostate cancer.
Oncotarget7(32)50997-51011(2016 Aug)
Bloom JE,McNeel DG

The SSX gene family: characterization of 9 complete genes.
International journal of cancer101(5)448-53(2002 Oct)
G?re AO,Wei IJ,Old LJ,Chen YT

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SSX2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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