SPAG1 cDNA ORF clone, Homo sapiens(human)

The following SPAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu17516 NM_172218.3
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu17516 NM_001374321.1
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu17516 NM_003114.5
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu17516 XM_011517242.2
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu17516 XM_011517243.2
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu60697 XM_011517240.2
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu60698 XM_011517241.2
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu60700 XM_011517245.2
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu98156 XM_017013754.1
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu98157 XM_017013755.1
Latest version!
Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
View Old Accession Versions >>
OHu17516 NM_003114.4 Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu17516 XM_011517243.1 Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu17516 XM_011517242.1 Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu17516 NM_172218.2 Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu17516
    Clone ID Related Accession (Same CDS sequence) NM_003114.4 , NM_001374321.1 , XM_011517243.2 , XM_011517242.2 , NM_172218.2 , NM_003114.5 , XM_011517243.1 , XM_011517242.1 , NM_172218.3
    Accession Version NM_003114.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB028984.1, AK057482.1, AF311312.1, BM557203.1, BX480304.1 and AI624411.1. On Mar 10, 2011 this sequence version replaced NM_003114.3. Transcript Variant: This variant (1) represents the longer transcript. ##Evidence-Data-START## Transcript exon combination :: AF311312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003105.2
    CDS285..3065
    Translation

    Target ORF information:

    RefSeq Version NM_003114.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003114.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17516
    Clone ID Related Accession (Same CDS sequence) NM_003114.4 , NM_001374321.1 , XM_011517243.2 , XM_011517242.2 , NM_172218.2 , NM_003114.5 , XM_011517243.1 , XM_011517242.1 , NM_172218.3
    Accession Version NM_001374321.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025647.9. On Sep 19, 2019 this sequence version replaced XM_011517242.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DRR138524.920673.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361250.1
    CDS94..2874
    Translation

    Target ORF information:

    RefSeq Version NM_001374321.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17516
    Clone ID Related Accession (Same CDS sequence) NM_003114.4 , NM_001374321.1 , XM_011517243.2 , XM_011517242.2 , NM_172218.2 , NM_003114.5 , XM_011517243.1 , XM_011517242.1 , NM_172218.3
    Accession Version NM_172218.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-03
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK057482.1, AF311312.1, BM557203.1, BX480304.1 and AI624411.1. This sequence is a reference standard in the RefSeqGene project. On Mar 10, 2011 this sequence version replaced NM_172218.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. ##Evidence-Data-START## CDS exon combination :: AF311312.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_757367.1
    CDS87..2867
    Translation

    Target ORF information:

    RefSeq Version NM_172218.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172218.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17516
    Clone ID Related Accession (Same CDS sequence) NM_003114.4 , NM_001374321.1 , XM_011517243.2 , XM_011517242.2 , NM_172218.2 , NM_003114.5 , XM_011517243.1 , XM_011517242.1 , NM_172218.3
    Accession Version NM_003114.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-19
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025647.9. On Sep 19, 2019 this sequence version replaced NM_003114.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF311312.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003105.2
    CDS33..2813
    Translation

    Target ORF information:

    RefSeq Version NM_003114.5
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003114.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17516
    Clone ID Related Accession (Same CDS sequence) NM_003114.4 , NM_001374321.1 , XM_011517243.2 , XM_011517242.2 , NM_172218.2 , NM_003114.5 , XM_011517243.1 , XM_011517242.1 , NM_172218.3
    Accession Version NM_172218.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025647.9. On Sep 19, 2019 this sequence version replaced NM_172218.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_757367.1
    CDS84..2864
    Translation

    Target ORF information:

    RefSeq Version NM_172218.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172218.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17516
    Clone ID Related Accession (Same CDS sequence) NM_003114.4 , NM_001374321.1 , XM_011517243.2 , XM_011517242.2 , NM_172218.2 , NM_003114.5 , XM_011517243.1 , XM_011517242.1 , NM_172218.3
    Accession Version XM_011517243.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011515545.1
    CDS23..2803
    Translation

    Target ORF information:

    RefSeq Version XM_011517243.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011517243.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17516
    Clone ID Related Accession (Same CDS sequence) NM_003114.4 , NM_001374321.1 , XM_011517243.2 , XM_011517242.2 , NM_172218.2 , NM_003114.5 , XM_011517243.1 , XM_011517242.1 , NM_172218.3
    Accession Version XM_011517242.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517242.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011515544.1
    CDS129..2909
    Translation

    Target ORF information:

    RefSeq Version XM_011517242.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011517242.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17516
    Clone ID Related Accession (Same CDS sequence) NM_003114.4 , NM_001374321.1 , XM_011517243.2 , XM_011517242.2 , NM_172218.2 , NM_003114.5 , XM_011517243.1 , XM_011517242.1 , NM_172218.3
    Accession Version XM_011517243.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product sperm-associated antigen 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011515545.1
    Translation

    Target ORF information:

    RefSeq Version XM_011517243.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011517243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17516
    Clone ID Related Accession (Same CDS sequence) NM_003114.4 , NM_001374321.1 , XM_011517243.2 , XM_011517242.2 , NM_172218.2 , NM_003114.5 , XM_011517243.1 , XM_011517242.1 , NM_172218.3
    Accession Version XM_011517242.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product sperm-associated antigen 1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011515544.1
    Translation

    Target ORF information:

    RefSeq Version XM_011517242.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011517242.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGAATTA TCAGAAAAGC TTAATTGATC TCAATAAAGT TATCCTACTA
    GATCCAAGTA TTATTGAGGC AAAGATGGAA CTGGAAGAGG TAACTAGACT CCTTAATCTT
    AAGGATAAGA CAGCACCATT CAACAAAGAA AAGGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAATGAAG GCAAGGAGGA GCCTGGAAGA CCTGCAGGGG AGGTCTCCAT GGGATGCCTT
    GCTTCTGAGA AGGGAGGCAA AAGCAGCAGG TCACCAGAAG ACCCTGAGAA ACTTCCGATA
    GCCAAGCCTA ATAATGCCTA TGAATTTGGT CAGATTATAA ATGCTCTCAG TACCAGGAAG
    GATAAAGAAG CCTGTGCACA TCTTTTAGCC ATCACTGCAC CAAAAGATTT GCCGATGTTT
    TTAAGTAACA AACTTGAAGG GGATACATTC CTTCTCCTCA TTCAGTCTCT GAAAAATAAT
    CTTATTGAAA AAGATCCCTC ATTGGTGTAT CAGCATCTTT TATACCTGAG TAAAGCAGAA
    AGGTTTAAGA TGATGTTGAC ACTAATTAGC AAGGGCCAAA AGGAGCTAAT TGAACAGCTG
    TTTGAGGACC TTTCGGACAC ACCAAACAAC CATTTTACTT TAGAAGATAT ACAGGCCCTA
    AAAAGGCAGT ATGAGCTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60697
    Clone ID Related Accession (Same CDS sequence) XM_011517240.2
    Accession Version XM_011517240.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517240.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGCTAT CAAGAATTTT AATGGAGCTG
    GATGGACCAA ATTGGCGGGA GAAGCTGTCA CCTATTCCTG CTGTGCCTGC TTCTGTGCCA
    CTGCAAGCTT GGCATCCGGC AAAAGAGATG ATCTCAAAAC AAGCAGGAGA CTCCAGCAGC
    CATCGCCAGC AGGGCATCAC AGATGAAAAA ACATTTAAAG CCCTTAAGGA AGAAGGAAAT
    CAATGTGTAA ATGACAAAAA CTATAAAGAC GCCCTCAGTA AATACAGCGA ATGCTTAAAG
    ATTAACAATA AGGAATGTGC CATATATACA AACAGAGCTC TCTGTTACTT GAAGCTGTGC
    CAGTTTGAAG AAGCAAAGCA GGACTGTGAT CAGGCACTTC AGCTAGCTGA TGGGAACGTG
    AAAGCCTTCT ATAGACGAGC TCTGGCTCAT AAAGGACTCA AGAATTATCA GAAAAGCTTA
    ATTGATCTCA ATAAAGTTAT CCTACTAGAT CCAAGTATTA TTGAGGCAAA GATGGAACTG
    GAAGAGGTAA CTAGACTCCT TAATCTTAAG GATAAGACAG CACCATTCAA CAAAGAAAAG
    GAGAGAAGGA AAATTGAGAT TCAAGAGGTG AATGAAGGCA AGGAGGAGCC TGGAAGACCT
    GCAGGGGAGG TCTCCATGGG ATGCCTTGCT TCTGAGAAGG GAGGCAAAAG CAGCAGGTCA
    CCAGAAGACC CTGAGAAACT TCCGATAGCC AAGCCTAATA ATGCCTATGA ATTTGGTCAG
    ATTATAAATG CTCTCAGTAC CAGGAAGGAT AAAGAAGCCT GTGCACATCT TTTAGCCATC
    ACTGCACCAA AAGATTTGCC GATGTTTTTA AGTAACAAAC TTGAAGGGGA TACATTCCTT
    CTCCTCATTC AGTCTCTGAA AAATAATCTT ATTGAAAAAG ATCCCTCATT GGTGTATCAG
    CATCTTTTAT ACCTGAGTAA AGCAGAAAGG TTTAAGATGA TGTTGACACT AATTAGCAAG
    GGCCAAAAGG AGCTAATTGA ACAGCTGTTT GAGGACCTTT CGGACACACC AAACAACCAT
    TTTACTTTAG AAGATATACA GGCCCTAAAA AGGCAGTATG AGCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011515542.1
    CDS87..2714
    Translation

    Target ORF information:

    RefSeq Version XM_011517240.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011517240.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGCTAT CAAGAATTTT AATGGAGCTG
    GATGGACCAA ATTGGCGGGA GAAGCTGTCA CCTATTCCTG CTGTGCCTGC TTCTGTGCCA
    CTGCAAGCTT GGCATCCGGC AAAAGAGATG ATCTCAAAAC AAGCAGGAGA CTCCAGCAGC
    CATCGCCAGC AGGGCATCAC AGATGAAAAA ACATTTAAAG CCCTTAAGGA AGAAGGAAAT
    CAATGTGTAA ATGACAAAAA CTATAAAGAC GCCCTCAGTA AATACAGCGA ATGCTTAAAG
    ATTAACAATA AGGAATGTGC CATATATACA AACAGAGCTC TCTGTTACTT GAAGCTGTGC
    CAGTTTGAAG AAGCAAAGCA GGACTGTGAT CAGGCACTTC AGCTAGCTGA TGGGAACGTG
    AAAGCCTTCT ATAGACGAGC TCTGGCTCAT AAAGGACTCA AGAATTATCA GAAAAGCTTA
    ATTGATCTCA ATAAAGTTAT CCTACTAGAT CCAAGTATTA TTGAGGCAAA GATGGAACTG
    GAAGAGGTAA CTAGACTCCT TAATCTTAAG GATAAGACAG CACCATTCAA CAAAGAAAAG
    GAGAGAAGGA AAATTGAGAT TCAAGAGGTG AATGAAGGCA AGGAGGAGCC TGGAAGACCT
    GCAGGGGAGG TCTCCATGGG ATGCCTTGCT TCTGAGAAGG GAGGCAAAAG CAGCAGGTCA
    CCAGAAGACC CTGAGAAACT TCCGATAGCC AAGCCTAATA ATGCCTATGA ATTTGGTCAG
    ATTATAAATG CTCTCAGTAC CAGGAAGGAT AAAGAAGCCT GTGCACATCT TTTAGCCATC
    ACTGCACCAA AAGATTTGCC GATGTTTTTA AGTAACAAAC TTGAAGGGGA TACATTCCTT
    CTCCTCATTC AGTCTCTGAA AAATAATCTT ATTGAAAAAG ATCCCTCATT GGTGTATCAG
    CATCTTTTAT ACCTGAGTAA AGCAGAAAGG TTTAAGATGA TGTTGACACT AATTAGCAAG
    GGCCAAAAGG AGCTAATTGA ACAGCTGTTT GAGGACCTTT CGGACACACC AAACAACCAT
    TTTACTTTAG AAGATATACA GGCCCTAAAA AGGCAGTATG AGCTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60698
    Clone ID Related Accession (Same CDS sequence) XM_011517241.2
    Accession Version XM_011517241.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2616bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517241.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGGTGAA TGAAGGCAAG GAGGAGCCTG GAAGACCTGC AGGGGAGGTC
    TCCATGGGAT GCCTTGCTTC TGAGAAGGGA GGCAAAAGCA GCAGGTCACC AGAAGACCCT
    GAGAAACTTC CGATAGCCAA GCCTAATAAT GCCTATGAAT TTGGTCAGAT TATAAATGCT
    CTCAGTACCA GGAAGGATAA AGAAGCCTGT GCACATCTTT TAGCCATCAC TGCACCAAAA
    GATTTGCCGA TGTTTTTAAG TAACAAACTT GAAGGGGATA CATTCCTTCT CCTCATTCAG
    TCTCTGAAAA ATAATCTTAT TGAAAAAGAT CCCTCATTGG TGTATCAGCA TCTTTTATAC
    CTGAGTAAAG CAGAAAGGTT TAAGATGATG TTGACACTAA TTAGCAAGGG CCAAAAGGAG
    CTAATTGAAC AGCTGTTTGA GGACCTTTCG GACACACCAA ACAACCATTT TACTTTAGAA
    GATATACAGG CCCTAAAAAG GCAGTATGAG CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011515543.1
    CDS87..2702
    Translation

    Target ORF information:

    RefSeq Version XM_011517241.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011517241.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGAAGT
    GAAATTGCAG ATGATCTAAG TATCTTATAT TCAAATAGAG CAGCATGTTA CCTAAAAGAA
    GGAAACTGCA GTGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA TCCATTCTCT
    ATGAAACCTC TTCTGAGGCG GGCGATGGCC TATGAAACTC TAGAGCAGTA TGGGAAAGCT
    TATGTGGATT ATAAAACAGT GTTGCAGATA GACTGTGGAC TCCAGCTAGC AAATGACAGT
    GTTAACAGGC TATCAAGAAT TTTAATGGAG CTGGATGGAC CAAATTGGCG GGAGAAGCTG
    TCACCTATTC CTGCTGTGCC TGCTTCTGTG CCACTGCAAG CTTGGCATCC GGCAAAAGAG
    ATGATCTCAA AACAAGCAGG AGACTCCAGC AGCCATCGCC AGCAGGGCAT CACAGATGAA
    AAAACATTTA AAGCCCTTAA GGAAGAAGGA AATCAATGTG TAAATGACAA AAACTATAAA
    GACGCCCTCA GTAAATACAG CGAATGCTTA AAGATTAACA ATAAGGAATG TGCCATATAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAAGCAAA GCAGGACTGT
    GATCAGGCAC TTCAGCTAGC TGATGGGAAC GTGAAAGCCT TCTATAGACG AGCTCTGGCT
    CATAAAGGAC TCAAGGTGAA TGAAGGCAAG GAGGAGCCTG GAAGACCTGC AGGGGAGGTC
    TCCATGGGAT GCCTTGCTTC TGAGAAGGGA GGCAAAAGCA GCAGGTCACC AGAAGACCCT
    GAGAAACTTC CGATAGCCAA GCCTAATAAT GCCTATGAAT TTGGTCAGAT TATAAATGCT
    CTCAGTACCA GGAAGGATAA AGAAGCCTGT GCACATCTTT TAGCCATCAC TGCACCAAAA
    GATTTGCCGA TGTTTTTAAG TAACAAACTT GAAGGGGATA CATTCCTTCT CCTCATTCAG
    TCTCTGAAAA ATAATCTTAT TGAAAAAGAT CCCTCATTGG TGTATCAGCA TCTTTTATAC
    CTGAGTAAAG CAGAAAGGTT TAAGATGATG TTGACACTAA TTAGCAAGGG CCAAAAGGAG
    CTAATTGAAC AGCTGTTTGA GGACCTTTCG GACACACCAA ACAACCATTT TACTTTAGAA
    GATATACAGG CCCTAAAAAG GCAGTATGAG CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60700
    Clone ID Related Accession (Same CDS sequence) XM_011517245.2
    Accession Version XM_011517245.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGGCTC
    TGGAACTTCA TCCATTCTCT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011515547.1
    CDS87..1550
    Translation

    Target ORF information:

    RefSeq Version XM_011517245.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011517245.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACCACCA AAGATTATCC ATCATTGTGG GGCTTTGGAA CAACAAAAAC ATTCAAAATT 
    CCCATTGAAC ATCTAGATTT CAAATACATT GAAAAATGTT CAGATGTTAA ACATCTGGAA
    AAAATTCTTT GCGTGCTCAG ATCTGGTGAG GAAGGATATT ATCCTGAACT TACAGAATTT
    TGTGAAAAGC ATCTTCAAGC CTTGGCCCCT GAAAGCAGAG CTTTGAGGAA AGATAAACCA
    GCAGCAACAG CAGCCAGTTT TACAGCTGAA GAATGGGAAA AAATTGATGG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAA GATAAAATGC ACTTTCATGA AACTGAGACA
    TTTCCAGCAA TGAAAGATAA TTTGCCTCCA GTTCGTGGTT CAAACAGCTG TCTTCATGTA
    GGCAAGGAAA AATATTCTAA AAGACCAACT AAAAAGAAAA CTCCAAGGGA TTACGCGGAA
    TGGGATAAAT TTGACGTGGA GAAGGAATGT TTAAAAATTG ATGAAGATTA CAAAGAAAAG
    ACGGTAATAG ACAAGTCACA CTTGTCTAAA ATTGAGACAA GAATAGATAC AGCAGGTCTA
    ACTGAGAAAG AAAAGGATTT TCTTGCCACT CGTGAAAAGG AGAAAGGAAA TGAAGCTTTC
    AACTCAGGAG ATTATGAAGA AGCAGTGATG TATTATACCA GGAGCATATC AGCGCTTCCC
    ACTGTAGTTG CCTATAACAA TCGAGCTCAA GCAGAAATCA AATTACAGAA CTGGAATAGT
    GCTTTTCAGG ATTGTGAAAA GGTCTTGGAG TTAGAACCTG GAAACGTAAA GGCTCTTCTG
    CGTCGTGCTA CTACATATAA ACATCAAAAC AAGCTCCGGG AAGCTACAGA AGATTTGAGT
    AAAGTACTAG ATGTTGAGCC TGATAATGAT TTGGCCAAGA AAACCTTGTC AGAGGTTGAA
    AGAGATCTGA AAAATTCTGA AGCTGCATCT GAGACTCAAA CCAAAGGGAA AAGGATGGTT
    ATTCAGGAAA TAGAAAACTC CGAAGATGAA GAAGGAAAAA GCGGAAGAAA ACATGAAGAT
    GGCGGTGGAG ATAAGAAGCC CGCGGAGCCG GCGGGAGCCG CGCGCGCCGC CCAGCCGTGC
    GTCATGGGCA ACATCCAGAA GAAGCTGACT GGCAAAGCCG AAGGCGGCAA GCGGCCGGCA
    AGGGGCGCGC CGCAGCGGGG CCAGACCCCG GAGGCCGGCG CGGACAAGCG GAGCCCACGG
    CGGGCCTCTG CGGCGGCGGC GGCGGGCGGC GGCGCCACCG GGCATCCGGG CGGCGGGCAG
    GGCGCGGAGA ACCCTGCCGG CCTGAAGAGC CAGGGCAACG AGCTGTTCCG AAGCGGGCAG
    TTCGCCGAGG CGGCCGGCAA GTACTCGGCG GCAATCGCGC TCCTGGAGCC AGCAGGGCTC
    TGGAACTTCA TCCATTCTCT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98156
    Clone ID Related Accession (Same CDS sequence) XM_017013754.1
    Accession Version XM_017013754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2886bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCCAATT GGTCCTCTGC CAGTGAAGGC GGGGAAGGTG AACCTGTGGC CGTCGGCAGA 
    TCTGCAGATC TGCCGGACTG CGGGTCGGGG CGTGGCAGGG GCCTAGTCCC CGCCCCGGCC
    GTCAGCCTGT CGCCTCTGGC TCGGGCCGGG CGACGAGACC CTCCCTCAGC ACCTGCAGTG
    CAGCCAGAGC CGGTGCGGCG GCCGCGACTT CCCCGACGCG GGGCGCTGAG AAGCAGTGAG
    TGCCTATCTG GTGAGGAAGG ATATTATCCT GAACTTACAG AATTTTGTGA AAAGCATCTT
    CAAGCCTTGG CCCCTGAAAG CAGAGCTTTG AGGAAAGATA AACCAGCAGC AACAGCAGCC
    AGTTTTACAG CTGAAGAATG GGAAAAAATT GATGGTGATA TAAAGAGTTG GGTATCAGAA
    ATTAAAAAAG AAGAAGATAA AATGCACTTT CATGAAACTG AGACATTTCC AGCAATGAAA
    GATAATTTGC CTCCAGTTCG TGGTTCAAAC AGCTGTCTTC ATGTAGGCAA GGAAAAATAT
    TCTAAAAGAC CAACTAAAAA GAAAACTCCA AGGGATTACG CGGAATGGGA TAAATTTGAC
    GTGGAGAAGG AATGTTTAAA AATTGATGAA GATTACAAAG AAAAGACGGT AATAGACAAG
    TCACACTTGT CTAAAATTGA GACAAGAATA GATACAGCAG GTCTAACTGA GAAAGAAAAG
    GATTTTCTTG CCACTCGTGA AAAGGAGAAA GGAAATGAAG CTTTCAACTC AGGAGATTAT
    GAAGAAGCAG TGATGTATTA TACCAGGAGC ATATCAGCGC TTCCCACTGT AGTTGCCTAT
    AACAATCGAG CTCAAGCAGA AATCAAATTA CAGAACTGGA ATAGTGCTTT TCAGGATTGT
    GAAAAGGTCT TGGAGTTAGA ACCTGGAAAC GTAAAGGCTC TTCTGCGTCG TGCTACTACA
    TATAAACATC AAAACAAGCT CCGGGAAGCT ACAGAAGATT TGAGTAAAGT ACTAGATGTT
    GAGCCTGATA ATGATTTGGC CAAGAAAACC TTGTCAGAGG TTGAAAGAGA TCTGAAAAAT
    TCTGAAGCTG CATCTGAGAC TCAAACCAAA GGGAAAAGGA TGGTTATTCA GGAAATAGAA
    AACTCCGAAG ATGAAGAAGG AAAAAGCGGA AGAAAACATG AAGATGGCGG TGGAGATAAG
    AAGCCCGCGG AGCCGGCGGG AGCCGCGCGC GCCGCCCAGC CGTGCGTCAT GGGCAACATC
    CAGAAGAAGC TGACTGGCAA AGCCGAAGGC GGCAAGCGGC CGGCAAGGGG CGCGCCGCAG
    CGGGGCCAGA CCCCGGAGGC CGGCGCGGAC AAGCGGAGCC CACGGCGGGC CTCTGCGGCG
    GCGGCGGCGG GCGGCGGCGC CACCGGGCAT CCGGGCGGCG GGCAGGGCGC GGAGAACCCT
    GCCGGCCTGA AGAGCCAGGG CAACGAGCTG TTCCGAAGCG GGCAGTTCGC CGAGGCGGCC
    GGCAAGTACT CGGCGGCAAT CGCGCTCCTG GAGCCAGCAG GAAGTGAAAT TGCAGATGAT
    CTAAGTATCT TATATTCAAA TAGAGCAGCA TGTTACCTAA AAGAAGGAAA CTGCAGTGGC
    TGCATTCAAG ATTGTAACAG GGCTCTGGAA CTTCATCCAT TCTCTATGAA ACCTCTTCTG
    AGGCGGGCGA TGGCCTATGA AACTCTAGAG CAGTATGGGA AAGCTTATGT GGATTATAAA
    ACAGTGTTGC AGATAGACTG TGGACTCCAG CTAGCAAATG ACAGTGTTAA CAGGCTATCA
    AGAATTTTAA TGGAGCTGGA TGGACCAAAT TGGCGGGAGA AGCTGTCACC TATTCCTGCT
    GTGCCTGCTT CTGTGCCACT GCAAGCTTGG CATCCGGCAA AAGAGATGAT CTCAAAACAA
    GCAGGAGACT CCAGCAGCCA TCGCCAGCAG GGCATCACAG ATGAAAAAAC ATTTAAAGCC
    CTTAAGGAAG AAGGAAATCA ATGTGTAAAT GACAAAAACT ATAAAGACGC CCTCAGTAAA
    TACAGCGAAT GCTTAAAGAT TAACAATAAG GAATGTGCCA TATATACAAA CAGAGCTCTC
    TGTTACTTGA AGCTGTGCCA GTTTGAAGAA GCAAAGCAGG ACTGTGATCA GGCACTTCAG
    CTAGCTGATG GGAACGTGAA AGCCTTCTAT AGACGAGCTC TGGCTCATAA AGGACTCAAG
    AATTATCAGA AAAGCTTAAT TGATCTCAAT AAAGTTATCC TACTAGATCC AAGTATTATT
    GAGGCAAAGA TGGAACTGGA AGAGGTAACT AGACTCCTTA ATCTTAAGGA TAAGACAGCA
    CCATTCAACA AAGAAAAGGA GAGAAGGAAA ATTGAGATTC AAGAGGTGAA TGAAGGCAAG
    GAGGAGCCTG GAAGACCTGC AGGGGAGGTC TCCATGGGAT GCCTTGCTTC TGAGAAGGGA
    GGCAAAAGCA GCAGGTCACC AGAAGACCCT GAGAAACTTC CGATAGCCAA GCCTAATAAT
    GCCTATGAAT TTGGTCAGAT TATAAATGCT CTCAGTACCA GGAAGGATAA AGAAGCCTGT
    GCACATCTTT TAGCCATCAC TGCACCAAAA GATTTGCCGA TGTTTTTAAG TAACAAACTT
    GAAGGGGATA CATTCCTTCT CCTCATTCAG TCTCTGAAAA ATAATCTTAT TGAAAAAGAT
    CCCTCATTGG TGTATCAGCA TCTTTTATAC CTGAGTAAAG CAGAAAGGTT TAAGATGATG
    TTGACACTAA TTAGCAAGGG CCAAAAGGAG CTAATTGAAC AGCTGTTTGA GGACCTTTCG
    GACACACCAA ACAACCATTT TACTTTAGAA GATATACAGG CCCTAAAAAG GCAGTATGAG
    CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869243.1
    CDS16..2901
    Translation

    Target ORF information:

    RefSeq Version XM_017013754.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017013754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCCCAATT GGTCCTCTGC CAGTGAAGGC GGGGAAGGTG AACCTGTGGC CGTCGGCAGA 
    TCTGCAGATC TGCCGGACTG CGGGTCGGGG CGTGGCAGGG GCCTAGTCCC CGCCCCGGCC
    GTCAGCCTGT CGCCTCTGGC TCGGGCCGGG CGACGAGACC CTCCCTCAGC ACCTGCAGTG
    CAGCCAGAGC CGGTGCGGCG GCCGCGACTT CCCCGACGCG GGGCGCTGAG AAGCAGTGAG
    TGCCTATCTG GTGAGGAAGG ATATTATCCT GAACTTACAG AATTTTGTGA AAAGCATCTT
    CAAGCCTTGG CCCCTGAAAG CAGAGCTTTG AGGAAAGATA AACCAGCAGC AACAGCAGCC
    AGTTTTACAG CTGAAGAATG GGAAAAAATT GATGGTGATA TAAAGAGTTG GGTATCAGAA
    ATTAAAAAAG AAGAAGATAA AATGCACTTT CATGAAACTG AGACATTTCC AGCAATGAAA
    GATAATTTGC CTCCAGTTCG TGGTTCAAAC AGCTGTCTTC ATGTAGGCAA GGAAAAATAT
    TCTAAAAGAC CAACTAAAAA GAAAACTCCA AGGGATTACG CGGAATGGGA TAAATTTGAC
    GTGGAGAAGG AATGTTTAAA AATTGATGAA GATTACAAAG AAAAGACGGT AATAGACAAG
    TCACACTTGT CTAAAATTGA GACAAGAATA GATACAGCAG GTCTAACTGA GAAAGAAAAG
    GATTTTCTTG CCACTCGTGA AAAGGAGAAA GGAAATGAAG CTTTCAACTC AGGAGATTAT
    GAAGAAGCAG TGATGTATTA TACCAGGAGC ATATCAGCGC TTCCCACTGT AGTTGCCTAT
    AACAATCGAG CTCAAGCAGA AATCAAATTA CAGAACTGGA ATAGTGCTTT TCAGGATTGT
    GAAAAGGTCT TGGAGTTAGA ACCTGGAAAC GTAAAGGCTC TTCTGCGTCG TGCTACTACA
    TATAAACATC AAAACAAGCT CCGGGAAGCT ACAGAAGATT TGAGTAAAGT ACTAGATGTT
    GAGCCTGATA ATGATTTGGC CAAGAAAACC TTGTCAGAGG TTGAAAGAGA TCTGAAAAAT
    TCTGAAGCTG CATCTGAGAC TCAAACCAAA GGGAAAAGGA TGGTTATTCA GGAAATAGAA
    AACTCCGAAG ATGAAGAAGG AAAAAGCGGA AGAAAACATG AAGATGGCGG TGGAGATAAG
    AAGCCCGCGG AGCCGGCGGG AGCCGCGCGC GCCGCCCAGC CGTGCGTCAT GGGCAACATC
    CAGAAGAAGC TGACTGGCAA AGCCGAAGGC GGCAAGCGGC CGGCAAGGGG CGCGCCGCAG
    CGGGGCCAGA CCCCGGAGGC CGGCGCGGAC AAGCGGAGCC CACGGCGGGC CTCTGCGGCG
    GCGGCGGCGG GCGGCGGCGC CACCGGGCAT CCGGGCGGCG GGCAGGGCGC GGAGAACCCT
    GCCGGCCTGA AGAGCCAGGG CAACGAGCTG TTCCGAAGCG GGCAGTTCGC CGAGGCGGCC
    GGCAAGTACT CGGCGGCAAT CGCGCTCCTG GAGCCAGCAG GAAGTGAAAT TGCAGATGAT
    CTAAGTATCT TATATTCAAA TAGAGCAGCA TGTTACCTAA AAGAAGGAAA CTGCAGTGGC
    TGCATTCAAG ATTGTAACAG GGCTCTGGAA CTTCATCCAT TCTCTATGAA ACCTCTTCTG
    AGGCGGGCGA TGGCCTATGA AACTCTAGAG CAGTATGGGA AAGCTTATGT GGATTATAAA
    ACAGTGTTGC AGATAGACTG TGGACTCCAG CTAGCAAATG ACAGTGTTAA CAGGCTATCA
    AGAATTTTAA TGGAGCTGGA TGGACCAAAT TGGCGGGAGA AGCTGTCACC TATTCCTGCT
    GTGCCTGCTT CTGTGCCACT GCAAGCTTGG CATCCGGCAA AAGAGATGAT CTCAAAACAA
    GCAGGAGACT CCAGCAGCCA TCGCCAGCAG GGCATCACAG ATGAAAAAAC ATTTAAAGCC
    CTTAAGGAAG AAGGAAATCA ATGTGTAAAT GACAAAAACT ATAAAGACGC CCTCAGTAAA
    TACAGCGAAT GCTTAAAGAT TAACAATAAG GAATGTGCCA TATATACAAA CAGAGCTCTC
    TGTTACTTGA AGCTGTGCCA GTTTGAAGAA GCAAAGCAGG ACTGTGATCA GGCACTTCAG
    CTAGCTGATG GGAACGTGAA AGCCTTCTAT AGACGAGCTC TGGCTCATAA AGGACTCAAG
    AATTATCAGA AAAGCTTAAT TGATCTCAAT AAAGTTATCC TACTAGATCC AAGTATTATT
    GAGGCAAAGA TGGAACTGGA AGAGGTAACT AGACTCCTTA ATCTTAAGGA TAAGACAGCA
    CCATTCAACA AAGAAAAGGA GAGAAGGAAA ATTGAGATTC AAGAGGTGAA TGAAGGCAAG
    GAGGAGCCTG GAAGACCTGC AGGGGAGGTC TCCATGGGAT GCCTTGCTTC TGAGAAGGGA
    GGCAAAAGCA GCAGGTCACC AGAAGACCCT GAGAAACTTC CGATAGCCAA GCCTAATAAT
    GCCTATGAAT TTGGTCAGAT TATAAATGCT CTCAGTACCA GGAAGGATAA AGAAGCCTGT
    GCACATCTTT TAGCCATCAC TGCACCAAAA GATTTGCCGA TGTTTTTAAG TAACAAACTT
    GAAGGGGATA CATTCCTTCT CCTCATTCAG TCTCTGAAAA ATAATCTTAT TGAAAAAGAT
    CCCTCATTGG TGTATCAGCA TCTTTTATAC CTGAGTAAAG CAGAAAGGTT TAAGATGATG
    TTGACACTAA TTAGCAAGGG CCAAAAGGAG CTAATTGAAC AGCTGTTTGA GGACCTTTCG
    GACACACCAA ACAACCATTT TACTTTAGAA GATATACAGG CCCTAAAAAG GCAGTATGAG
    CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98157
    Clone ID Related Accession (Same CDS sequence) XM_017013755.1
    Accession Version XM_017013755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sperm-associated antigen 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCACTTTC ATGAAACTGA GACATTTCCA GCAATGAAAG ATAATTTGCC TCCAGTTCGT 
    GGTTCAAACA GCTGTCTTCA TGTAGGCAAG GAAAAATATT CTAAAAGACC AACTAAAAAG
    AAAACTCCAA GGGATTACGC GGAATGGGAT AAATTTGACG TGGAGAAGGA ATGTTTAAAA
    ATTGATGAAG ATTACAAAGA AAAGACGGTA ATAGACAAGT CACACTTGTC TAAAATTGAG
    ACAAGAATAG ATACAGCAGG TCTAACTGAG AAAGAAAAGG ATTTTCTTGC CACTCGTGAA
    AAGGAGAAAG GAAATGAAGC TTTCAACTCA GGAGATTATG AAGAAGCAGT GATGTATTAT
    ACCAGGAGCA TATCAGCGCT TCCCACTGTA GTTGCCTATA ACAATCGAGC TCAAGCAGAA
    ATCAAATTAC AGAACTGGAA TAGTGCTTTT CAGGATTGTG AAAAGGTCTT GGAGTTAGAA
    CCTGGAAACG TAAAGGCTCT TCTGCGTCGT GCTACTACAT ATAAACATCA AAACAAGCTC
    CGGGAAGCTA CAGAAGATTT GAGTAAAGTA CTAGATGTTG AGCCTGATAA TGATTTGGCC
    AAGAAAACCT TGTCAGAGGT TGAAAGAGAT CTGAAAAATT CTGAAGCTGC ATCTGAGACT
    CAAACCAAAG GGAAAAGGAT GGTTATTCAG GAAATAGAAA ACTCCGAAGA TGAAGAAGGA
    AAAAGCGGAA GAAAACATGA AGATGGCGGT GGAGATAAGA AGCCCGCGGA GCCGGCGGGA
    GCCGCGCGCG CCGCCCAGCC GTGCGTCATG GGCAACATCC AGAAGAAGCT GACTGGCAAA
    GCCGAAGGCG GCAAGCGGCC GGCAAGGGGC GCGCCGCAGC GGGGCCAGAC CCCGGAGGCC
    GGCGCGGACA AGCGGAGCCC ACGGCGGGCC TCTGCGGCGG CGGCGGCGGG CGGCGGCGCC
    ACCGGGCATC CGGGCGGCGG GCAGGGCGCG GAGAACCCTG CCGGCCTGAA GAGCCAGGGC
    AACGAGCTGT TCCGAAGCGG GCAGTTCGCC GAGGCGGCCG GCAAGTACTC GGCGGCAATC
    GCGCTCCTGG AGCCAGCAGG AAGTGAAATT GCAGATGATC TAAGTATCTT ATATTCAAAT
    AGAGCAGCAT GTTACCTAAA AGAAGGAAAC TGCAGTGGCT GCATTCAAGA TTGTAACAGG
    GCTCTGGAAC TTCATCCATT CTCTATGAAA CCTCTTCTGA GGCGGGCGAT GGCCTATGAA
    ACTCTAGAGC AGTATGGGAA AGCTTATGTG GATTATAAAA CAGTGTTGCA GATAGACTGT
    GGACTCCAGC TAGCAAATGA CAGTGTTAAC AGGCTATCAA GAATTTTAAT GGAGCTGGAT
    GGACCAAATT GGCGGGAGAA GCTGTCACCT ATTCCTGCTG TGCCTGCTTC TGTGCCACTG
    CAAGCTTGGC ATCCGGCAAA AGAGATGATC TCAAAACAAG CAGGAGACTC CAGCAGCCAT
    CGCCAGCAGG GCATCACAGA TGAAAAAACA TTTAAAGCCC TTAAGGAAGA AGGAAATCAA
    TGTGTAAATG ACAAAAACTA TAAAGACGCC CTCAGTAAAT ACAGCGAATG CTTAAAGATT
    AACAATAAGG AATGTGCCAT ATATACAAAC AGAGCTCTCT GTTACTTGAA GCTGTGCCAG
    TTTGAAGAAG CAAAGCAGGA CTGTGATCAG GCACTTCAGC TAGCTGATGG GAACGTGAAA
    GCCTTCTATA GACGAGCTCT GGCTCATAAA GGACTCAAGA ATTATCAGAA AAGCTTAATT
    GATCTCAATA AAGTTATCCT ACTAGATCCA AGTATTATTG AGGCAAAGAT GGAACTGGAA
    GAGGTAACTA GACTCCTTAA TCTTAAGGAT AAGACAGCAC CATTCAACAA AGAAAAGGAG
    AGAAGGAAAA TTGAGATTCA AGAGGTGAAT GAAGGCAAGG AGGAGCCTGG AAGACCTGCA
    GGGGAGGTCT CCATGGGATG CCTTGCTTCT GAGAAGGGAG GCAAAAGCAG CAGGTCACCA
    GAAGACCCTG AGAAACTTCC GATAGCCAAG CCTAATAATG CCTATGAATT TGGTCAGATT
    ATAAATGCTC TCAGTACCAG GAAGGATAAA GAAGCCTGTG CACATCTTTT AGCCATCACT
    GCACCAAAAG ATTTGCCGAT GTTTTTAAGT AACAAACTTG AAGGGGATAC ATTCCTTCTC
    CTCATTCAGT CTCTGAAAAA TAATCTTATT GAAAAAGATC CCTCATTGGT GTATCAGCAT
    CTTTTATACC TGAGTAAAGC AGAAAGGTTT AAGATGATGT TGACACTAAT TAGCAAGGGC
    CAAAAGGAGC TAATTGAACA GCTGTTTGAG GACCTTTCGG ACACACCAAA CAACCATTTT
    ACTTTAGAAG ATATACAGGC CCTAAAAAGG CAGTATGAGC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016869244.1
    CDS410..2854
    Translation

    Target ORF information:

    RefSeq Version XM_017013755.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sperm associated antigen 1 (SPAG1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017013755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCACTTTC ATGAAACTGA GACATTTCCA GCAATGAAAG ATAATTTGCC TCCAGTTCGT 
    GGTTCAAACA GCTGTCTTCA TGTAGGCAAG GAAAAATATT CTAAAAGACC AACTAAAAAG
    AAAACTCCAA GGGATTACGC GGAATGGGAT AAATTTGACG TGGAGAAGGA ATGTTTAAAA
    ATTGATGAAG ATTACAAAGA AAAGACGGTA ATAGACAAGT CACACTTGTC TAAAATTGAG
    ACAAGAATAG ATACAGCAGG TCTAACTGAG AAAGAAAAGG ATTTTCTTGC CACTCGTGAA
    AAGGAGAAAG GAAATGAAGC TTTCAACTCA GGAGATTATG AAGAAGCAGT GATGTATTAT
    ACCAGGAGCA TATCAGCGCT TCCCACTGTA GTTGCCTATA ACAATCGAGC TCAAGCAGAA
    ATCAAATTAC AGAACTGGAA TAGTGCTTTT CAGGATTGTG AAAAGGTCTT GGAGTTAGAA
    CCTGGAAACG TAAAGGCTCT TCTGCGTCGT GCTACTACAT ATAAACATCA AAACAAGCTC
    CGGGAAGCTA CAGAAGATTT GAGTAAAGTA CTAGATGTTG AGCCTGATAA TGATTTGGCC
    AAGAAAACCT TGTCAGAGGT TGAAAGAGAT CTGAAAAATT CTGAAGCTGC ATCTGAGACT
    CAAACCAAAG GGAAAAGGAT GGTTATTCAG GAAATAGAAA ACTCCGAAGA TGAAGAAGGA
    AAAAGCGGAA GAAAACATGA AGATGGCGGT GGAGATAAGA AGCCCGCGGA GCCGGCGGGA
    GCCGCGCGCG CCGCCCAGCC GTGCGTCATG GGCAACATCC AGAAGAAGCT GACTGGCAAA
    GCCGAAGGCG GCAAGCGGCC GGCAAGGGGC GCGCCGCAGC GGGGCCAGAC CCCGGAGGCC
    GGCGCGGACA AGCGGAGCCC ACGGCGGGCC TCTGCGGCGG CGGCGGCGGG CGGCGGCGCC
    ACCGGGCATC CGGGCGGCGG GCAGGGCGCG GAGAACCCTG CCGGCCTGAA GAGCCAGGGC
    AACGAGCTGT TCCGAAGCGG GCAGTTCGCC GAGGCGGCCG GCAAGTACTC GGCGGCAATC
    GCGCTCCTGG AGCCAGCAGG AAGTGAAATT GCAGATGATC TAAGTATCTT ATATTCAAAT
    AGAGCAGCAT GTTACCTAAA AGAAGGAAAC TGCAGTGGCT GCATTCAAGA TTGTAACAGG
    GCTCTGGAAC TTCATCCATT CTCTATGAAA CCTCTTCTGA GGCGGGCGAT GGCCTATGAA
    ACTCTAGAGC AGTATGGGAA AGCTTATGTG GATTATAAAA CAGTGTTGCA GATAGACTGT
    GGACTCCAGC TAGCAAATGA CAGTGTTAAC AGGCTATCAA GAATTTTAAT GGAGCTGGAT
    GGACCAAATT GGCGGGAGAA GCTGTCACCT ATTCCTGCTG TGCCTGCTTC TGTGCCACTG
    CAAGCTTGGC ATCCGGCAAA AGAGATGATC TCAAAACAAG CAGGAGACTC CAGCAGCCAT
    CGCCAGCAGG GCATCACAGA TGAAAAAACA TTTAAAGCCC TTAAGGAAGA AGGAAATCAA
    TGTGTAAATG ACAAAAACTA TAAAGACGCC CTCAGTAAAT ACAGCGAATG CTTAAAGATT
    AACAATAAGG AATGTGCCAT ATATACAAAC AGAGCTCTCT GTTACTTGAA GCTGTGCCAG
    TTTGAAGAAG CAAAGCAGGA CTGTGATCAG GCACTTCAGC TAGCTGATGG GAACGTGAAA
    GCCTTCTATA GACGAGCTCT GGCTCATAAA GGACTCAAGA ATTATCAGAA AAGCTTAATT
    GATCTCAATA AAGTTATCCT ACTAGATCCA AGTATTATTG AGGCAAAGAT GGAACTGGAA
    GAGGTAACTA GACTCCTTAA TCTTAAGGAT AAGACAGCAC CATTCAACAA AGAAAAGGAG
    AGAAGGAAAA TTGAGATTCA AGAGGTGAAT GAAGGCAAGG AGGAGCCTGG AAGACCTGCA
    GGGGAGGTCT CCATGGGATG CCTTGCTTCT GAGAAGGGAG GCAAAAGCAG CAGGTCACCA
    GAAGACCCTG AGAAACTTCC GATAGCCAAG CCTAATAATG CCTATGAATT TGGTCAGATT
    ATAAATGCTC TCAGTACCAG GAAGGATAAA GAAGCCTGTG CACATCTTTT AGCCATCACT
    GCACCAAAAG ATTTGCCGAT GTTTTTAAGT AACAAACTTG AAGGGGATAC ATTCCTTCTC
    CTCATTCAGT CTCTGAAAAA TAATCTTATT GAAAAAGATC CCTCATTGGT GTATCAGCAT
    CTTTTATACC TGAGTAAAGC AGAAAGGTTT AAGATGATGT TGACACTAAT TAGCAAGGGC
    CAAAAGGAGC TAATTGAACA GCTGTTTGAG GACCTTTCGG ACACACCAAA CAACCATTTT
    ACTTTAGAAG ATATACAGGC CCTAAAAAGG CAGTATGAGC TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.