Spag1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_011245357.1	XP_011243659.1	sperm-associated antigen 1 isoform X1
XM_017316637.1	XP_017172126.1	sperm-associated antigen 1 isoform X3
NM_012031.3	NP_036161.2	sperm-associated antigen 1
XM_017316634.1	XP_017172123.1	sperm-associated antigen 1 isoform X1
NM_001359984.1	NP_001346913.1	sperm-associated antigen 1 isoform 1
XM_017316636.1	XP_017172125.1	sperm-associated antigen 1 isoform X2
XM_017316633.1	XP_017172122.1	sperm-associated antigen 1 isoform X1
XM_017316635.1	XP_017172124.1	sperm-associated antigen 1 isoform X1
XM_006520075.2	XP_006520138.1	sperm-associated antigen 1 isoform X2
XM_006520076.2	XP_006520139.1	sperm-associated antigen 1 isoform X1
NM_001359981.1	NP_001346910.1	sperm-associated antigen 1 isoform 1
XM_006520079.3	XP_006520142.1	sperm-associated antigen 1 isoform X3
XM_006520078.3	XP_006520141.1	sperm-associated antigen 1 isoform X3
XM_006520076.3	XP_006520139.1	sperm-associated antigen 1 isoform X2
NM_012031.4	NP_036161.2	sperm-associated antigen 1 isoform 3
XM_017316632.1	XP_017172121.1	sperm-associated antigen 1 isoform X1
XM_017316634.2	XP_017172123.1	sperm-associated antigen 1 isoform X1
XM_006520078.4	XP_006520141.1	sperm-associated antigen 1 isoform X3
NM_001359980.1	NP_001346909.1	sperm-associated antigen 1 isoform 4
NM_001359982.1	NP_001346911.1	sperm-associated antigen 1 isoform 1
NM_001359983.1	NP_001346912.1	sperm-associated antigen 1 isoform 2
XM_017316636.2	XP_017172125.1	sperm-associated antigen 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09365	XM_017316636.2 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu50694	XM_017316634.2 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu50696	XM_006520078.4 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu09365	NM_012031.4 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu09365	NM_001359980.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50694	NM_001359984.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50694	NM_001359982.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50694	NM_001359981.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50696	NM_001359983.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu09365	XM_011245357.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu09365	XM_006520076.3 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu09365	XM_006520075.2 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50694	XM_017316632.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50694	XM_017316633.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50694	XM_017316635.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50696	XM_017316637.1 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu50696	XM_006520079.3 Latest version!	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu09365	XM_006520076.2	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu09365	NM_012031.3	Mus musculus sperm associated antigen 1 (Spag1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50694	XM_017316634.1	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu50696	XM_006520078.3	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu09365	XM_017316636.1	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

Spag1 ( XM_017316636.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172125.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu09365
Clone ID Related Accession (Same CDS sequence)	XM_011245357.1 , NM_012031.3 , XM_017316636.1 , XM_006520075.2 , XM_006520076.2 , XM_006520076.3 , NM_012031.4 , NM_001359980.1 , XM_017316636.2
Accession Version	XM_017316636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172125.1
CDS	109..2814
Translation	MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_017316636.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316636.2

ORF Insert Sequence:

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_017316634.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172123.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50694
Clone ID Related Accession (Same CDS sequence)	XM_017316634.1 , NM_001359984.1 , XM_017316633.1 , XM_017316635.1 , NM_001359981.1 , XM_017316632.1 , XM_017316634.2 , NM_001359982.1
Accession Version	XM_017316634.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316634.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172123.1
CDS	332..3073
Translation	MQFSILQHETLTMTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_017316634.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316634.2

ORF Insert Sequence:

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_006520078.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520141.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X5, mRNA.

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CloneID	OMu50696
Clone ID Related Accession (Same CDS sequence)	XM_017316637.1 , XM_006520079.3 , XM_006520078.3 , XM_006520078.4 , NM_001359983.1
Accession Version	XM_006520078.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520078.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520141.1
CDS	1465..3471
Translation	MYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_006520078.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520078.4

ORF Insert Sequence:

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( NM_012031.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036161.2 Mus musculus sperm associated antigen 1 (Spag1), mRNA.

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CloneID	OMu09365
Clone ID Related Accession (Same CDS sequence)	XM_011245357.1 , NM_012031.3 , XM_017316636.1 , XM_006520075.2 , XM_006520076.2 , XM_006520076.3 , NM_012031.4 , NM_001359980.1 , XM_017316636.2
Accession Version	NM_012031.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC046313.1. On Jun 10, 2007 this sequence version replaced NM_012031.2. ##Evidence-Data-START## Transcript exon combination :: BC046313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036161.2
CDS	235..2940
Translation	MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	NM_012031.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012031.3

ORF Insert Sequence:

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( NM_012031.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036161.2 Mus musculus sperm associated antigen 1 (Spag1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu09365
Clone ID Related Accession (Same CDS sequence)	XM_011245357.1 , NM_012031.3 , XM_017316636.1 , XM_006520075.2 , XM_006520076.2 , XM_006520076.3 , NM_012031.4 , NM_001359980.1 , XM_017316636.2
Accession Version	NM_012031.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC046313.1, BB706348.1 and AI843673.1. On Jan 23, 2018 this sequence version replaced NM_012031.3. Transcript Variant: This variant (3) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC046313.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036161.2
CDS	235..2940
Translation	MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	NM_012031.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012031.4

ORF Insert Sequence:

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( NM_001359980.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346909.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu09365
Clone ID Related Accession (Same CDS sequence)	XM_011245357.1 , NM_012031.3 , XM_017316636.1 , XM_006520075.2 , XM_006520076.2 , XM_006520076.3 , NM_012031.4 , NM_001359980.1 , XM_017316636.2
Accession Version	NM_001359980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121577.3. On Jan 24, 2018 this sequence version replaced XM_006520075.2. Transcript Variant: This variant (4) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF181252.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346909.1
CDS	727..3432
Translation	MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	NM_001359980.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359980.1

ORF Insert Sequence:

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( NM_001359984.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346913.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OMu50694
Clone ID Related Accession (Same CDS sequence)	XM_017316634.1 , NM_001359984.1 , XM_017316633.1 , XM_017316635.1 , NM_001359981.1 , XM_017316632.1 , XM_017316634.2 , NM_001359982.1
Accession Version	NM_001359984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121577.3. On Jan 24, 2018 this sequence version replaced XM_017316635.1. Transcript Variant: This variant (6) differs in the 5' UTR compared to variant 1. Variants 1, 5 and 6 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.115440.1, SRR1660817.242396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346913.1
CDS	472..3213
Translation	MQFSILQHETLTMTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	NM_001359984.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359984.1

ORF Insert Sequence:

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( NM_001359981.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346910.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu50694
Clone ID Related Accession (Same CDS sequence)	XM_017316634.1 , NM_001359984.1 , XM_017316633.1 , XM_017316635.1 , NM_001359981.1 , XM_017316632.1 , XM_017316634.2 , NM_001359982.1
Accession Version	NM_001359981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121577.3. On Jan 24, 2018 this sequence version replaced XM_017316633.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1, 5 and 6 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.48944.1, SRR6116558.170279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346910.1
CDS	537..3278
Translation	MQFSILQHETLTMTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	NM_001359981.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359981.1

ORF Insert Sequence:

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( NM_001359982.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346911.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu50694
Clone ID Related Accession (Same CDS sequence)	XM_017316634.1 , NM_001359984.1 , XM_017316633.1 , XM_017316635.1 , NM_001359981.1 , XM_017316632.1 , XM_017316634.2 , NM_001359982.1
Accession Version	NM_001359982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121577.3. On Jan 24, 2018 this sequence version replaced XM_017316632.1. Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 1. Variants 1, 5 and 6 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.255013.1, SRR1660819.75097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346911.1
CDS	568..3309
Translation	MQFSILQHETLTMTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	NM_001359982.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359982.1

ORF Insert Sequence:

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( NM_001359983.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346912.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu50696
Clone ID Related Accession (Same CDS sequence)	XM_017316637.1 , XM_006520079.3 , XM_006520078.3 , XM_006520078.4 , NM_001359983.1
Accession Version	NM_001359983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121577.3. On Jan 24, 2018 this sequence version replaced XM_017316637.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.143845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346912.1
CDS	1283..3289
Translation	MYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	NM_001359983.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359983.1

ORF Insert Sequence:

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_011245357.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243659.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu09365
Clone ID Related Accession (Same CDS sequence)	XM_011245357.1 , NM_012031.3 , XM_017316636.1 , XM_006520075.2 , XM_006520076.2 , XM_006520076.3 , NM_012031.4 , NM_001359980.1 , XM_017316636.2
Accession Version	XM_011245357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	sperm-associated antigen 1 isoform X1
Comment

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011243659.1

Translation

MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_011245357.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245357.1

ORF Insert Sequence:

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_017316636.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172125.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu09365
Clone ID Related Accession (Same CDS sequence)	XM_011245357.1 , NM_012031.3 , XM_017316636.1 , XM_006520075.2 , XM_006520076.2 , XM_006520076.3 , NM_012031.4 , NM_001359980.1 , XM_017316636.2
Accession Version	XM_017316636.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172125.1
CDS	95..2800
Translation	MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_017316636.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316636.1

ORF Insert Sequence:

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_006520075.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520138.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu09365
Clone ID Related Accession (Same CDS sequence)	XM_011245357.1 , NM_012031.3 , XM_017316636.1 , XM_006520075.2 , XM_006520076.2 , XM_006520076.3 , NM_012031.4 , NM_001359980.1 , XM_017316636.2
Accession Version	XM_006520075.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006520075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520138.1
CDS	727..3432
Translation	MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_006520075.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520075.2

ORF Insert Sequence:

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_006520076.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520139.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu09365
Clone ID Related Accession (Same CDS sequence)	XM_011245357.1 , NM_012031.3 , XM_017316636.1 , XM_006520075.2 , XM_006520076.2 , XM_006520076.3 , NM_012031.4 , NM_001359980.1 , XM_017316636.2
Accession Version	XM_006520076.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	sperm-associated antigen 1 isoform X1
Comment

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006520139.1

Translation

MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_006520076.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520076.2

ORF Insert Sequence:

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_006520076.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520139.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu09365
Clone ID Related Accession (Same CDS sequence)	XM_011245357.1 , NM_012031.3 , XM_017316636.1 , XM_006520075.2 , XM_006520076.2 , XM_006520076.3 , NM_012031.4 , NM_001359980.1 , XM_017316636.2
Accession Version	XM_006520076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520139.1
CDS	70..2775
Translation	MTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_006520076.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520076.3

ORF Insert Sequence:

ATGACTGCTA AGGCTAAGGA CTGTCCATCA TTGTGGGGCT TTGGAACAAC AAAAACATTT 
AAAATTCCCA TCGAACATTT GGATTTCAAG TATATTGAAA ACTGTTCAGA TGTGAAACAC 
CTGGAAAAAA TTCTTTATGT GCTGAGGTCT GGTGAAGAAG GCTACTATCC TGAACTCACG 
GAATTTTGCG AGAAGTGCCT CACCAACTTG GCTCCTAAAA GTCGAGCTCT GAGGAAGGAC 
AAGCCTGCAG AGACAGCATC CAGCTTTTCA GCGGAAGAGT GGGAAAAAAT CGACAGTGAT 
CTAAAGAGTT GGGTGTCAGA AATCAAAAGA GAGGAAAATA CATGTCACTT CCATGACCCT 
GAAAACCATC CCGGAGTGGA AGATCCTCTG CCTCCTGTCC GCGGCTCCAC CTGCTGCCCT 
CACAGTGGCA AGGAAACATA TTCTAAAAGT AAGACAGCTA AGAAGAGGAT TCCAAGGGAT 
TATGCAGAAT GGGATAAATT TGATGTGGAA AAAGAATGTT CAAAGATTGA TGAGGATTAC 
AAAGAAAAGA CTGTCATAAA CAACAAGGCT CATCTGTCTA AAATTGAGAC AAAAATAGAA 
ACAGCAGGTC TGACTGAGAA GGAAAAGAGT TTTCTTGCCA ATCGTGAAAA GGGAAAAGGG 
AACGAGGCTT TCTACTCTGG GGACTATGAA GAGGCCGTGA TGTATTACAC CAGGAGCTTA 
TCAGCCCTTC CCACCGCCAT TGCCTATAAC AATCGAGCTC AGGCCGAAAT CAAGTTGCAG 
AGATGGAGCA GTGCTCTTGA AGACTGTGAG AAGGCCTTGG AGCTAGATCC CGGAAACGTA 
AAAGCTCTCC TGAGACGAGC CACTACATAT AAACATCAAA ACAAGCTCCA GGAAGCTGTG 
GACGACCTGA GAAAGGTGCT GCAGGTGGAG CCGGACAACG ACTTGGCCAA GAAGACCTTA 
TCAGAGGTTG AAAGAGATCT GAAGAATTCA GAGCCTGTCT CTGAGCTCCA AACCAAAGGG 
AAGAGGATGG TTATTGAAGA AGTTGAAAAC TCGGGAGATG AAGGTGGAAA GGGAAGCGCA 
GATGAGCGTG AAGATGGTGG CTCGGATGAG GCGGCCATGG GCAACATTCA GAAGAAGCTG 
ATGGTCCGCC GGAGCGAGGG TGGCAGGCGG TCGCGGCGGG GCCGGACACC GGGGCCCCGA 
GCTGAGCAGC AGGGAGGCCT GCGAGAGACA GCGACGGCCA GCACCGGCGA CAGTCACTAC 
CCGGAGGAGC CGCGAGCCGC TGACAACCCC AGCGGCCTCA AGAGACGGGG CAACGAGCTG 
TTCCGCGGCG GGCAATTCGC AGAGGCAGCC GCGCAATACT CGGTGGCGAT AGCGCAGCTG 
GAACCCACAG GAAGTGCAAA TGCGGATGAG CTAAGCATCT TGTATTCAAA TAGAGCGGCA 
TGCTACCTCA AAGAAGGGAA CTGCAGGGAC TGCATCCAGG ATTGTAACAG GGCTCTGGAA 
CTTCATCCGT TCTCAGTGAA GCCTCTTCTG CGGCGAGCAA TGGCCTATGA GACCTTAGAG 
CAATACAGGA ACGCATATGT GGACTATAAA ACAGTGCTGC AGATAGATTG TGGGATCCAG 
CTGGCGAGCG ACAGTGCTAA CAGAATAGCA CGAATCTTAA CAGAGCTAGA TGGATCAAAA 
TGGCGGGAGA GACTGCCACC CATCCCAGCT GTACCCACAT CTGAGCCACT GCGAGTTTGG 
CTCCCTGCTG CAGAGACCCC AGACCAAGAT CCCTGTCCCA ACAACTGCAT GCCGAGCATC 
ACAGATGAAA AAATGTTTCA AGCCCTCAAG GAAGAAGGAA ATCAACTTGT AAAGGACAAA 
AATTATAAAG ATGCCATTAG TAAATACAAT GAATGCTTAA AGATTAACAG CAAGGCATGT 
GCCATCTATA CAAACAGGGC TCTCTGTTAC CTGAAGCTGG GCCAGTTTGA AGAGGCAAAG 
CTGGACTGTG AGCAGGCGCT TCAGATAGAC GGCGAGAATG TGAAAGCCAG CCACAGACTA 
GCGCTCGCCC AGAAAGGACT CGAGAACTGC CGGGAAAGCG GGGTGGACCC CAGTCAAGTC 
CTCCTAAGCC CAGATTCCAG TGAAGCCGCC CGGCACCTGG ACACTAAGAA TGACACAGCA 
CCTCCCAGCA AAGGAAGGGA GAGGAGGAGA ATCCAAGTCC AAGAGGTGGA TGGCAGCAGT 
GATGAGGAGC CTGAGAGACC TGCAGAGGCC TCTGCTACCT CCGCACCTGC CAGAGATGGT 
GTGGAAGATG GAGGGTCTGC AGAACCAGCA GAAAAACTCG ACGTCTCCAA GCCTACTAAT 
GCCTATGAGT TTGGACAGGT CCTAAGCACT ATCAGTGCCA GGAAAGATGA AGAGGCCTGT 
GCACATCTTT TAGCCATCAC TGCACCCAAA GACCTGCCAC TGTTGCTAAG TAACAAACTT 
GAGGGGGACA CGTTCCTCCT CCTCATTCAG TCACTTAAAA GTCACCTTGT GGCTAAAGAC 
CCCTCCCTGG TGTATGAGCA TCTCCTGTAT CTGAGCAAAG CAGAGAGGTT TAAGACGATG 
CTGACACTAA TTAACAAAGG CCAAAAGGAG CAGATGGCGC AGCTGTTTGA TGGTCTGTCA 
GACACACAGT CCGACGGTTT GACGGCAGAG GATGTACAGG CCCTAAGAAG GCAGTATGAG 
CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_017316634.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172123.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu50694
Clone ID Related Accession (Same CDS sequence)	XM_017316634.1 , NM_001359984.1 , XM_017316633.1 , XM_017316635.1 , NM_001359981.1 , XM_017316632.1 , XM_017316634.2 , NM_001359982.1
Accession Version	XM_017316634.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172123.1
CDS	674..3415
Translation	MQFSILQHETLTMTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_017316634.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316634.1

ORF Insert Sequence:

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_017316633.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172122.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu50694
Clone ID Related Accession (Same CDS sequence)	XM_017316634.1 , NM_001359984.1 , XM_017316633.1 , XM_017316635.1 , NM_001359981.1 , XM_017316632.1 , XM_017316634.2 , NM_001359982.1
Accession Version	XM_017316633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172122.1
CDS	1283..4024
Translation	MQFSILQHETLTMTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_017316633.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316633.1

ORF Insert Sequence:

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_017316635.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172124.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu50694
Clone ID Related Accession (Same CDS sequence)	XM_017316634.1 , NM_001359984.1 , XM_017316633.1 , XM_017316635.1 , NM_001359981.1 , XM_017316632.1 , XM_017316634.2 , NM_001359982.1
Accession Version	XM_017316635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172124.1
CDS	472..3213
Translation	MQFSILQHETLTMTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_017316635.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316635.1

ORF Insert Sequence:

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_017316632.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172121.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50694
Clone ID Related Accession (Same CDS sequence)	XM_017316634.1 , NM_001359984.1 , XM_017316633.1 , XM_017316635.1 , NM_001359981.1 , XM_017316632.1 , XM_017316634.2 , NM_001359982.1
Accession Version	XM_017316632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172121.1
CDS	568..3309
Translation	MQFSILQHETLTMTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYIENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFSAEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYSKSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTEKEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_017316632.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316632.1

ORF Insert Sequence:

ATGCAGTTTA GCATTCTCCA ACATGAAACC CTAACCATGA CTGCTAAGGC TAAGGACTGT 
CCATCATTGT GGGGCTTTGG AACAACAAAA ACATTTAAAA TTCCCATCGA ACATTTGGAT 
TTCAAGTATA TTGAAAACTG TTCAGATGTG AAACACCTGG AAAAAATTCT TTATGTGCTG 
AGGTCTGGTG AAGAAGGCTA CTATCCTGAA CTCACGGAAT TTTGCGAGAA GTGCCTCACC 
AACTTGGCTC CTAAAAGTCG AGCTCTGAGG AAGGACAAGC CTGCAGAGAC AGCATCCAGC 
TTTTCAGCGG AAGAGTGGGA AAAAATCGAC AGTGATCTAA AGAGTTGGGT GTCAGAAATC 
AAAAGAGAGG AAAATACATG TCACTTCCAT GACCCTGAAA ACCATCCCGG AGTGGAAGAT 
CCTCTGCCTC CTGTCCGCGG CTCCACCTGC TGCCCTCACA GTGGCAAGGA AACATATTCT 
AAAAGTAAGA CAGCTAAGAA GAGGATTCCA AGGGATTATG CAGAATGGGA TAAATTTGAT 
GTGGAAAAAG AATGTTCAAA GATTGATGAG GATTACAAAG AAAAGACTGT CATAAACAAC 
AAGGCTCATC TGTCTAAAAT TGAGACAAAA ATAGAAACAG CAGGTCTGAC TGAGAAGGAA 
AAGAGTTTTC TTGCCAATCG TGAAAAGGGA AAAGGGAACG AGGCTTTCTA CTCTGGGGAC 
TATGAAGAGG CCGTGATGTA TTACACCAGG AGCTTATCAG CCCTTCCCAC CGCCATTGCC 
TATAACAATC GAGCTCAGGC CGAAATCAAG TTGCAGAGAT GGAGCAGTGC TCTTGAAGAC 
TGTGAGAAGG CCTTGGAGCT AGATCCCGGA AACGTAAAAG CTCTCCTGAG ACGAGCCACT 
ACATATAAAC ATCAAAACAA GCTCCAGGAA GCTGTGGACG ACCTGAGAAA GGTGCTGCAG 
GTGGAGCCGG ACAACGACTT GGCCAAGAAG ACCTTATCAG AGGTTGAAAG AGATCTGAAG 
AATTCAGAGC CTGTCTCTGA GCTCCAAACC AAAGGGAAGA GGATGGTTAT TGAAGAAGTT 
GAAAACTCGG GAGATGAAGG TGGAAAGGGA AGCGCAGATG AGCGTGAAGA TGGTGGCTCG 
GATGAGGCGG CCATGGGCAA CATTCAGAAG AAGCTGATGG TCCGCCGGAG CGAGGGTGGC 
AGGCGGTCGC GGCGGGGCCG GACACCGGGG CCCCGAGCTG AGCAGCAGGG AGGCCTGCGA 
GAGACAGCGA CGGCCAGCAC CGGCGACAGT CACTACCCGG AGGAGCCGCG AGCCGCTGAC 
AACCCCAGCG GCCTCAAGAG ACGGGGCAAC GAGCTGTTCC GCGGCGGGCA ATTCGCAGAG 
GCAGCCGCGC AATACTCGGT GGCGATAGCG CAGCTGGAAC CCACAGGAAG TGCAAATGCG 
GATGAGCTAA GCATCTTGTA TTCAAATAGA GCGGCATGCT ACCTCAAAGA AGGGAACTGC 
AGGGACTGCA TCCAGGATTG TAACAGGGCT CTGGAACTTC ATCCGTTCTC AGTGAAGCCT 
CTTCTGCGGC GAGCAATGGC CTATGAGACC TTAGAGCAAT ACAGGAACGC ATATGTGGAC 
TATAAAACAG TGCTGCAGAT AGATTGTGGG ATCCAGCTGG CGAGCGACAG TGCTAACAGA 
ATAGCACGAA TCTTAACAGA GCTAGATGGA TCAAAATGGC GGGAGAGACT GCCACCCATC 
CCAGCTGTAC CCACATCTGA GCCACTGCGA GTTTGGCTCC CTGCTGCAGA GACCCCAGAC 
CAAGATCCCT GTCCCAACAA CTGCATGCCG AGCATCACAG ATGAAAAAAT GTTTCAAGCC 
CTCAAGGAAG AAGGAAATCA ACTTGTAAAG GACAAAAATT ATAAAGATGC CATTAGTAAA 
TACAATGAAT GCTTAAAGAT TAACAGCAAG GCATGTGCCA TCTATACAAA CAGGGCTCTC 
TGTTACCTGA AGCTGGGCCA GTTTGAAGAG GCAAAGCTGG ACTGTGAGCA GGCGCTTCAG 
ATAGACGGCG AGAATGTGAA AGCCAGCCAC AGACTAGCGC TCGCCCAGAA AGGACTCGAG 
AACTGCCGGG AAAGCGGGGT GGACCCCAGT CAAGTCCTCC TAAGCCCAGA TTCCAGTGAA 
GCCGCCCGGC ACCTGGACAC TAAGAATGAC ACAGCACCTC CCAGCAAAGG AAGGGAGAGG 
AGGAGAATCC AAGTCCAAGA GGTGGATGGC AGCAGTGATG AGGAGCCTGA GAGACCTGCA 
GAGGCCTCTG CTACCTCCGC ACCTGCCAGA GATGGTGTGG AAGATGGAGG GTCTGCAGAA 
CCAGCAGAAA AACTCGACGT CTCCAAGCCT ACTAATGCCT ATGAGTTTGG ACAGGTCCTA 
AGCACTATCA GTGCCAGGAA AGATGAAGAG GCCTGTGCAC ATCTTTTAGC CATCACTGCA 
CCCAAAGACC TGCCACTGTT GCTAAGTAAC AAACTTGAGG GGGACACGTT CCTCCTCCTC 
ATTCAGTCAC TTAAAAGTCA CCTTGTGGCT AAAGACCCCT CCCTGGTGTA TGAGCATCTC 
CTGTATCTGA GCAAAGCAGA GAGGTTTAAG ACGATGCTGA CACTAATTAA CAAAGGCCAA 
AAGGAGCAGA TGGCGCAGCT GTTTGATGGT CTGTCAGACA CACAGTCCGA CGGTTTGACG 
GCAGAGGATG TACAGGCCCT AAGAAGGCAG TATGAGCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_017316637.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172126.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X10, mRNA.

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CloneID	OMu50696
Clone ID Related Accession (Same CDS sequence)	XM_017316637.1 , XM_006520079.3 , XM_006520078.3 , XM_006520078.4 , NM_001359983.1
Accession Version	XM_017316637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172126.1
CDS	2029..4035
Translation	MYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_017316637.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316637.1

ORF Insert Sequence:

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_006520079.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520142.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu50696
Clone ID Related Accession (Same CDS sequence)	XM_017316637.1 , XM_006520079.3 , XM_006520078.3 , XM_006520078.4 , NM_001359983.1
Accession Version	XM_006520079.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520142.1
CDS	1064..3070
Translation	MYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_006520079.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520079.3

ORF Insert Sequence:

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag1 ( XM_006520078.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520141.1 Mus musculus sperm associated antigen 1 (Spag1), transcript variant X11, mRNA.

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CloneID	OMu50696
Clone ID Related Accession (Same CDS sequence)	XM_017316637.1 , XM_006520079.3 , XM_006520078.3 , XM_006520078.4 , NM_001359983.1
Accession Version	XM_006520078.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520078.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520141.1
CDS	3606..5612
Translation	MYYTRSLSALPTAIAYNNRAQAEIKLQRWSSALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSEVERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKLMVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGNELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCNRALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILTELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEEGNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQIDGENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRERRRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQVLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLVYEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL

Target ORF information:

RefSeq Version	XM_006520078.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 1 (Spag1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520078.3

ORF Insert Sequence:

ATGTATTACA CCAGGAGCTT ATCAGCCCTT CCCACCGCCA TTGCCTATAA CAATCGAGCT 
CAGGCCGAAA TCAAGTTGCA GAGATGGAGC AGTGCTCTTG AAGACTGTGA GAAGGCCTTG 
GAGCTAGATC CCGGAAACGT AAAAGCTCTC CTGAGACGAG CCACTACATA TAAACATCAA 
AACAAGCTCC AGGAAGCTGT GGACGACCTG AGAAAGGTGC TGCAGGTGGA GCCGGACAAC 
GACTTGGCCA AGAAGACCTT ATCAGAGGTT GAAAGAGATC TGAAGAATTC AGAGCCTGTC 
TCTGAGCTCC AAACCAAAGG GAAGAGGATG GTTATTGAAG AAGTTGAAAA CTCGGGAGAT 
GAAGGTGGAA AGGGAAGCGC AGATGAGCGT GAAGATGGTG GCTCGGATGA GGCGGCCATG 
GGCAACATTC AGAAGAAGCT GATGGTCCGC CGGAGCGAGG GTGGCAGGCG GTCGCGGCGG 
GGCCGGACAC CGGGGCCCCG AGCTGAGCAG CAGGGAGGCC TGCGAGAGAC AGCGACGGCC 
AGCACCGGCG ACAGTCACTA CCCGGAGGAG CCGCGAGCCG CTGACAACCC CAGCGGCCTC 
AAGAGACGGG GCAACGAGCT GTTCCGCGGC GGGCAATTCG CAGAGGCAGC CGCGCAATAC 
TCGGTGGCGA TAGCGCAGCT GGAACCCACA GGAAGTGCAA ATGCGGATGA GCTAAGCATC 
TTGTATTCAA ATAGAGCGGC ATGCTACCTC AAAGAAGGGA ACTGCAGGGA CTGCATCCAG 
GATTGTAACA GGGCTCTGGA ACTTCATCCG TTCTCAGTGA AGCCTCTTCT GCGGCGAGCA 
ATGGCCTATG AGACCTTAGA GCAATACAGG AACGCATATG TGGACTATAA AACAGTGCTG 
CAGATAGATT GTGGGATCCA GCTGGCGAGC GACAGTGCTA ACAGAATAGC ACGAATCTTA 
ACAGAGCTAG ATGGATCAAA ATGGCGGGAG AGACTGCCAC CCATCCCAGC TGTACCCACA 
TCTGAGCCAC TGCGAGTTTG GCTCCCTGCT GCAGAGACCC CAGACCAAGA TCCCTGTCCC 
AACAACTGCA TGCCGAGCAT CACAGATGAA AAAATGTTTC AAGCCCTCAA GGAAGAAGGA 
AATCAACTTG TAAAGGACAA AAATTATAAA GATGCCATTA GTAAATACAA TGAATGCTTA 
AAGATTAACA GCAAGGCATG TGCCATCTAT ACAAACAGGG CTCTCTGTTA CCTGAAGCTG 
GGCCAGTTTG AAGAGGCAAA GCTGGACTGT GAGCAGGCGC TTCAGATAGA CGGCGAGAAT 
GTGAAAGCCA GCCACAGACT AGCGCTCGCC CAGAAAGGAC TCGAGAACTG CCGGGAAAGC 
GGGGTGGACC CCAGTCAAGT CCTCCTAAGC CCAGATTCCA GTGAAGCCGC CCGGCACCTG 
GACACTAAGA ATGACACAGC ACCTCCCAGC AAAGGAAGGG AGAGGAGGAG AATCCAAGTC 
CAAGAGGTGG ATGGCAGCAG TGATGAGGAG CCTGAGAGAC CTGCAGAGGC CTCTGCTACC 
TCCGCACCTG CCAGAGATGG TGTGGAAGAT GGAGGGTCTG CAGAACCAGC AGAAAAACTC 
GACGTCTCCA AGCCTACTAA TGCCTATGAG TTTGGACAGG TCCTAAGCAC TATCAGTGCC 
AGGAAAGATG AAGAGGCCTG TGCACATCTT TTAGCCATCA CTGCACCCAA AGACCTGCCA 
CTGTTGCTAA GTAACAAACT TGAGGGGGAC ACGTTCCTCC TCCTCATTCA GTCACTTAAA 
AGTCACCTTG TGGCTAAAGA CCCCTCCCTG GTGTATGAGC ATCTCCTGTA TCTGAGCAAA 
GCAGAGAGGT TTAAGACGAT GCTGACACTA ATTAACAAAG GCCAAAAGGA GCAGATGGCG 
CAGCTGTTTG ATGGTCTGTC AGACACACAG TCCGACGGTT TGACGGCAGA GGATGTACAG 
GCCCTAAGAA GGCAGTATGA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Spag1
Entrez Gene ID	26942
Full Name	sperm associated antigen 1
Synonyms	tpis
General protein information	Preferred Names sperm associated antigen 1 Names sperm-associated antigen 1 TPR-containing protein involved in spermatogenesis infertility-related sperm protein Spag-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 B3.1

Rattus norvegicus (Norway rat)	Spag1	NP_001012116.1
Drosophila melanogaster (fruit fly)	CG18472	NP_651514.1
Homo sapiens (human)	SPAG1	NP_757367.1
Danio rerio (zebrafish)	spag1a	NP_001082875.2
	SPAG1	XP_001097135.1
Gallus gallus (chicken)	SPAG1	XP_418360.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010786	XP_318524.3
Canis lupus familiaris (dog)	SPAG1	XP_005628013.1
Bos taurus (cattle)	SPAG1	XP_002692844.2
Mus musculus (house mouse)	Spag1	NP_036161.2
Pan troglodytes (chimpanzee)	SPAG1	XP_519885.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Spag1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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