SPAG1 cDNA ORF clone, Canis lupus familiaris(dog)

The following SPAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe293072 XM_022427113.1
Latest version!
Canis lupus familiaris sperm associated antigen 1 (SPAG1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe39609 XM_014118515.1
Latest version!
Canis lupus familiaris sperm associated antigen 1 (SPAG1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe293072
    Clone ID Related Accession (Same CDS sequence) XM_022427113.1
    Accession Version XM_022427113.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product LOW QUALITY PROTEIN: sperm-associated antigen 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACTGCCA AAGATTATCC GTCATTTTGG GGCTTTGGAA CAACAAAAAT GTTTAAAATT 
    CCTGTTGAAC ATTTGGATTT CAAGTATATT GAAAAATGTT CAGATGTTAA ACACTTGGAA
    AAAATTTTTT GTGTGCTCAG GTCTGGTGAG GAAGGATATT ATCCGGAACT TACGGAATTT
    TGTGAAAAGC GCCTTAAAGG CTTGGCTCCT GAAAGTAGAG CTTTGAGGAA AGATAAACCT
    CCAGCAACAG CATCCAGTTT TACAGCAGAA GAATGGAGAC ATATTGACAG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAT GAAGTATACT TCCATGAGAC TGAAACATTT
    CCAGCAATGG AAGATAATTT GCCTCCAGTT CGTGGTTCAA ACAGCCGTCT TCATGTTAGT
    AAGGAAAAAT ATTCTAATAG TAGACCAACT AAAAAGAAAA TTCCGAGGGA TTATGCAGAA
    TGGGATAAAT TTGACGTGGA AAAGGAATGT TCAAAAATTG ATGAAGATTA CAAAGAAAAA
    ACTGTAGTAA ACAACAAGTC ATATTTGTCT AAAATTGAGA CAAGAATAGG CACAGCAGGT
    CTAACTGAGA AGGAAAAGAC TTGCCTTGCT ACTCGTGAAA AGGAAAAAGG AAATGAAGCT
    TTCAACTCAG GAGACTATGA AGAGGCTGTG ATGTATTATA CTAGGAGTAT ATCAGTGCTT
    CCCACCGTGG TGGCCTACAA CAATCGGGCT CAAGCAGAAC TCAAACTACA GAACTGGAAC
    AGTGCTTTTT GGGATTGTGA AAAGGTCTTG GAATTAGAAC CTGGAAACAT AAAAGCTCTT
    CTTCGCCGTG CCACTACCTA TAAACATCAA AACAAGCTTC AGGAGGCTCT CGAAGATTTG
    AATAAAGTAC TGAATGTTGA ACCTGATAAT GAATTGGCCA AAAAGACCTT GTTGGAGGTT
    GAGAGAGATA TGAAAAATTC TGAACCTGCA TCTAAGACTC AAACCAAAGG GACGAGGATG
    GTTATCCAGG AAGTAGAAAA TTCAGAGGAT GAAGGTGGAA AGGACAGTGG AAGAACACAC
    AAAGACGGCA GTGGAGTTAA GAGGTACCTT GGGTGCAATC ACTGTATTCA AGGGTTGGAA
    ACTCGGTGCG TTCGTGAATG GTCGGTGCCA GGAGGGCACG TATTTTGTGA GGGGCACCTC
    GCCACGGACG CCTTGACCCG CGGCGACTTG GAGGAGCGGT GCTGCGGCCC GGAGCCGCTC
    GGCGTTCAGC GAGTTACTCC GCCCCCGGCC GCCGCGGTAG GTAGTTCCTC CCGCCCCGCG
    GCCTTGAAGA GCCAGGGCAA CGAGCTGTTC AAAAGCGGCC AGTTCGCCGA GGCGGCCCTC
    AAGTACTCGG CGGCGATCGC GCGGCTGGAG CCTGCAGGGA GTGGAAGTGC AGACGATCTA
    AGTGTCTTAT ATGCAAATAG AGCGGCGTGT TACCTGAAGG AAGGAAACTG CGGCGGCTGC
    ATTCAAGATT GTAACAGGGC TCTGGAACTT CACCCATTCT CAGTCAAACC TCTTCTTCGT
    CGAGCGATGG CCTATGAAAC TCTAGAGCAA TATCAGAAGG CCTATGTGGA TTACAAAACC
    GTGTTGCAGA TAGACTGCAA AATCCAGCTG GCAAATGACA GTATTAACAG AATAACAAGG
    ATTTTGATGA ATCTCGATGG GCCAAGCTGG CGGGAGAAGC TATCACCCAT TCCTGCCGTG
    CCCACCTCTG CGCATTTGCG AGCTTGGCAG CTGGCAGTAG AGCCGCCACC CAACCAAGTG
    GGAGATTCCT GCAGCCGTCC ACAGCCTGGC ATCACAGATG AAAAAATGTT TAAAAACCTT
    AAAGAAGAAG GAAATCAATG TGTAAAGGAC AAAAACTATA AAGATGCCCT TAGTAAATAC
    AGTGAATGCT TAACGATTAA CAACAAGGAA TGTGCCATTT ATACAAACAG AGCTCTCTGT
    TACTTGAAGC TGTGCCAGTT TGAAGAGGCA AAGCAGGACT GCGATCGGGC CCTTCAGATA
    GACAACGGGA ATGTGAAAGC ATGCTATCGA CGAGCCCTTG CTCACAAAGG ACTTAAGGAT
    TATCAGAAAA GTTTAAACGA TCTCAATAAA GTTCTCCTAC TAGATTCAAG TATTGTTGAG
    GCAAAGGTGG AACTGGAAGA GGTAACCAGA TTCCTTAACA TGAAGGATAA TATAGCATCA
    TTCAACAAAG AAAAAGAGAG AAGGAAAATT GAGATTCAAG AGGTGAGTGA AGGCCAGGAA
    GAAGAACCTG AAAGGACCTC GGAGGAGCTC TTCACTCACT GCATGGCCTC TGAGAATGGG
    GACACAACTA ATGGGCCACC AGAATGCGAT GAAGAACTAC CGATTACCAA GCCTAACAAT
    GCCTATGAAT TTGGTCAGAT TATAAATGCT ATTAGTATGA GGAATGATAA AGAAGCCTGT
    GCACACCTTT TAGCCATCAC TGAACCAAAA GATTTGCCAG TGTTGTTGAG TAACAAACTT
    GAAGGGGATA CGTTCTTCCT CCTCATTCAG TCTCTGAAAA ATAATCTTAT TGGAAAAGAT
    CCCTCACTGG TGTATCAGCA TCTTTTATAT CTGAGTAAAG CAGAAAGGTT CATGATGATG
    TTGACCCTAA TTAGCAAGAG CCAAAAGGAA CAAATTGAAC AGCTCTTTGA TGATCTCTCA
    GACACACCAA ATAAACATTT TACTTTGGAA GATGTACATG CCCTAAAAAG GAAGTATGAG
    CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022282821.1
    CDS77..2842
    Translation

    Target ORF information:

    RefSeq Version XM_022427113.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris sperm associated antigen 1 (SPAG1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022427113.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACTGCCA AAGATTATCC GTCATTTTGG GGCTTTGGAA CAACAAAAAT GTTTAAAATT 
    CCTGTTGAAC ATTTGGATTT CAAGTATATT GAAAAATGTT CAGATGTTAA ACACTTGGAA
    AAAATTTTTT GTGTGCTCAG GTCTGGTGAG GAAGGATATT ATCCGGAACT TACGGAATTT
    TGTGAAAAGC GCCTTAAAGG CTTGGCTCCT GAAAGTAGAG CTTTGAGGAA AGATAAACCT
    CCAGCAACAG CATCCAGTTT TACAGCAGAA GAATGGAGAC ATATTGACAG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAT GAAGTATACT TCCATGAGAC TGAAACATTT
    CCAGCAATGG AAGATAATTT GCCTCCAGTT CGTGGTTCAA ACAGCCGTCT TCATGTTAGT
    AAGGAAAAAT ATTCTAATAG TAGACCAACT AAAAAGAAAA TTCCGAGGGA TTATGCAGAA
    TGGGATAAAT TTGACGTGGA AAAGGAATGT TCAAAAATTG ATGAAGATTA CAAAGAAAAA
    ACTGTAGTAA ACAACAAGTC ATATTTGTCT AAAATTGAGA CAAGAATAGG CACAGCAGGT
    CTAACTGAGA AGGAAAAGAC TTGCCTTGCT ACTCGTGAAA AGGAAAAAGG AAATGAAGCT
    TTCAACTCAG GAGACTATGA AGAGGCTGTG ATGTATTATA CTAGGAGTAT ATCAGTGCTT
    CCCACCGTGG TGGCCTACAA CAATCGGGCT CAAGCAGAAC TCAAACTACA GAACTGGAAC
    AGTGCTTTTT GGGATTGTGA AAAGGTCTTG GAATTAGAAC CTGGAAACAT AAAAGCTCTT
    CTTCGCCGTG CCACTACCTA TAAACATCAA AACAAGCTTC AGGAGGCTCT CGAAGATTTG
    AATAAAGTAC TGAATGTTGA ACCTGATAAT GAATTGGCCA AAAAGACCTT GTTGGAGGTT
    GAGAGAGATA TGAAAAATTC TGAACCTGCA TCTAAGACTC AAACCAAAGG GACGAGGATG
    GTTATCCAGG AAGTAGAAAA TTCAGAGGAT GAAGGTGGAA AGGACAGTGG AAGAACACAC
    AAAGACGGCA GTGGAGTTAA GAGGTACCTT GGGTGCAATC ACTGTATTCA AGGGTTGGAA
    ACTCGGTGCG TTCGTGAATG GTCGGTGCCA GGAGGGCACG TATTTTGTGA GGGGCACCTC
    GCCACGGACG CCTTGACCCG CGGCGACTTG GAGGAGCGGT GCTGCGGCCC GGAGCCGCTC
    GGCGTTCAGC GAGTTACTCC GCCCCCGGCC GCCGCGGTAG GTAGTTCCTC CCGCCCCGCG
    GCCTTGAAGA GCCAGGGCAA CGAGCTGTTC AAAAGCGGCC AGTTCGCCGA GGCGGCCCTC
    AAGTACTCGG CGGCGATCGC GCGGCTGGAG CCTGCAGGGA GTGGAAGTGC AGACGATCTA
    AGTGTCTTAT ATGCAAATAG AGCGGCGTGT TACCTGAAGG AAGGAAACTG CGGCGGCTGC
    ATTCAAGATT GTAACAGGGC TCTGGAACTT CACCCATTCT CAGTCAAACC TCTTCTTCGT
    CGAGCGATGG CCTATGAAAC TCTAGAGCAA TATCAGAAGG CCTATGTGGA TTACAAAACC
    GTGTTGCAGA TAGACTGCAA AATCCAGCTG GCAAATGACA GTATTAACAG AATAACAAGG
    ATTTTGATGA ATCTCGATGG GCCAAGCTGG CGGGAGAAGC TATCACCCAT TCCTGCCGTG
    CCCACCTCTG CGCATTTGCG AGCTTGGCAG CTGGCAGTAG AGCCGCCACC CAACCAAGTG
    GGAGATTCCT GCAGCCGTCC ACAGCCTGGC ATCACAGATG AAAAAATGTT TAAAAACCTT
    AAAGAAGAAG GAAATCAATG TGTAAAGGAC AAAAACTATA AAGATGCCCT TAGTAAATAC
    AGTGAATGCT TAACGATTAA CAACAAGGAA TGTGCCATTT ATACAAACAG AGCTCTCTGT
    TACTTGAAGC TGTGCCAGTT TGAAGAGGCA AAGCAGGACT GCGATCGGGC CCTTCAGATA
    GACAACGGGA ATGTGAAAGC ATGCTATCGA CGAGCCCTTG CTCACAAAGG ACTTAAGGAT
    TATCAGAAAA GTTTAAACGA TCTCAATAAA GTTCTCCTAC TAGATTCAAG TATTGTTGAG
    GCAAAGGTGG AACTGGAAGA GGTAACCAGA TTCCTTAACA TGAAGGATAA TATAGCATCA
    TTCAACAAAG AAAAAGAGAG AAGGAAAATT GAGATTCAAG AGGTGAGTGA AGGCCAGGAA
    GAAGAACCTG AAAGGACCTC GGAGGAGCTC TTCACTCACT GCATGGCCTC TGAGAATGGG
    GACACAACTA ATGGGCCACC AGAATGCGAT GAAGAACTAC CGATTACCAA GCCTAACAAT
    GCCTATGAAT TTGGTCAGAT TATAAATGCT ATTAGTATGA GGAATGATAA AGAAGCCTGT
    GCACACCTTT TAGCCATCAC TGAACCAAAA GATTTGCCAG TGTTGTTGAG TAACAAACTT
    GAAGGGGATA CGTTCTTCCT CCTCATTCAG TCTCTGAAAA ATAATCTTAT TGGAAAAGAT
    CCCTCACTGG TGTATCAGCA TCTTTTATAT CTGAGTAAAG CAGAAAGGTT CATGATGATG
    TTGACCCTAA TTAGCAAGAG CCAAAAGGAA CAAATTGAAC AGCTCTTTGA TGATCTCTCA
    GACACACCAA ATAAACATTT TACTTTGGAA GATGTACATG CCCTAAAAAG GAAGTATGAG
    CTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39609
    Clone ID Related Accession (Same CDS sequence) XM_014118515.1
    Accession Version XM_014118515.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product LOW QUALITY PROTEIN: sperm-associated antigen 1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726082.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGCCA AAGATTATCC GTCATTTTGG GGCTTTGGAA CAACAAAAAT GTTTAAAATT 
    CCTGTTGAAC ATTTGGATTT CAAGTATATT GAAAAATGTT CAGATGTTAA ACACTTGGAA
    AAAATTTTTT GTGTGCTCAG GTCTGGTGAG GAAGGATATT ATCCGGAACT TACGGAATTT
    TGTGAAAAGC GCCTTAAAGG CTTGGCTCCT GAAAGTAGAG CTTTGAGGAA AGATAAACCT
    CCAGCAACAG CATCCAGTTT TACAGCAGAA GAATGGAGAC ATATTGACAG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAT GAAGTATACT TCCATGAGAC TGAAACATTT
    CCAGCAATGG AAGATAATTT GCCTCCAGTT CGTGGTTCAA ACAGCCGTCT TCATGTTAGT
    AAGGAAAAAT ATTCTAATAG TAGACCAACT AAAAAGAAAA TTCCGAGGGA TTATGCAGAA
    TGGGATAAAT TTGACGTGGA AAAGGAATGT TCAAAAATTG ATGAAGATTA CAAAGAAAAA
    ACTGTAGTAA ACAACAAGTC ATATTTGTCT AAAATTGAGA CAAGAATAGG CACAGCAGGT
    CTAACTGAGA AGGAAAAGAC TTGCCTTGCT ACTCGTGAAA AGGAAAAAGG AAATGAAGCT
    TTCAACTCAG GAGACTATGA AGAGGCTGTG ATGTATTATA CTAGGAGTAT ATCAGTGCTT
    CCCACCGTGG TGGCCTACAA CAATCGGGCT CAAGCAGAAC TCAAACTACA GAACTGGAAC
    AGTGCTTTTT GGGATTGTGA AAAGGTCTTG GAATTAGAAC CTGGAAACAT AAAAGCTCTT
    CTTCGCCGTG CCACTACCTA TAAACATCAA AACAAGCTTC AGGAGGCTCT CGAAGATTTG
    AATAAAGTAC TGAATGTTGA ACCTGATAAT GAATTGGCCA AAAAGACCTT GTTGGAGGTT
    GAGAGAGATA TGAAAAATTC TGAACCTGCA TCTAAGACTC AAACCAAAGG GACGAGGATG
    GTTATCCAGG AAGTAGAAAA TTCAGAGGAT GAAGGTGGAA AGGACAGTGG AAGAACACAC
    AAAGACGGCA GTGGAGTTAA GAAGCAGGGT ACCTTGGGTG CAATCACTGT ATTCAAGGGT
    TGGAAACTCG GTGCGTTCGT GAATGGTCGG TGCCAGGAGG GCACGTATTT TGTGAGGGGC
    ACCTCGCCAC GGACGCCTAG GCCGCCGAGC CGGCTGCAGC CGGGAGCCCC GCCGCGCCAA
    GCGCCATGGG CAATCATCCA GAAGAAGCTG TCGGGCGAGG GCGAGGGCGG CGGCAGCGGC
    CGGAGAGGGG CGCGCCGCGA TGGGGCCGGG GGACGTGGGT CCGAACGGAC AACTGGGACT
    ACTGCTAGCG GCCCCGCGGC CTTGAAGAGC CAGGGCAACG AGCTGTTCAA AAGCGGCCAG
    TTCGCCGAGG CGGCCCTCAA GTACTCGGCG GCGATCGCGC GGCTGGAGCC TGCAGGGAGT
    GGAAGTGCAG ACGATCTAAG TGTCTTATAT GCAAATAGAG CGGCGTGTTA CCTGAAGGAA
    GGAAACTGCG GCGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA CCCATTCTCA
    GTCAAACCTC TTCTTCGTCG AGCGATGGCC TATGAAACTC TAGAGCAATA TCAGAAGGCC
    TATGTGGATT ACAAAACCGT GTTGCAGATA GACTGCAAAA TCCAGCTGGC AAATGACAGT
    ATTAACAGAA TAACAAGGAT TTTGATGAAT CTCGATGGGC CAAGCTGGCG GGAGAAGCTA
    TCACCCATTC CTGCCGTGCC CACCTCTGCG CATTTGCGAG CTTGGCAGCT GGCAGTAGAG
    CCGCCACCCA ACCAAGTGGG AGATTCCTGC AGCCGTCCAC AGCCTGGCAT CACAGATGAA
    AAAATGTTTA AAAACCTTAA AGAAGAAGGA AATCAATGTG TAAAGGACAA AAACTATAAA
    GATGCCCTTA GTAAATACAG TGAATGCTTA ACGATTAACA ACAAGGAATG TGCCATTTAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAGGCAAA GCAGGACTGC
    GATCGGGCCC TTCAGATAGA CAACGGGAAT GTGAAAGCAT GCTATCGACG AGCCCTTGCT
    CACAAAGGAC TTAAGGATTA TCAGAAAAGT TTAAACGATC TCAATAAAGT TCTCCTACTA
    GATTCAAGTA TTGTTGAGGC AAAGGTGGAA CTGGAAGAGG TAACCAGATT CCTTAACATG
    AAGGATAATA TAGCATCATT CAACAAAGAA AAAGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAGTGAAG GCCAGGAAGA AGAACCTGAA AGGACCTCGG AGGAGCTCTT CACTCACTGC
    ATGGCCTCTG AGAATGGGGA CACAACTAAT GGGCCACCAG AATGCGATGA AGAACTACCG
    ATTACCAAGC CTAACAATGC CTATGAATTT GGTCAGATTA TAAATGCTAT TAGTATGAGG
    AATGATAAAG AAGCCTGTGC ACACCTTTTA GCCATCACTG AACCAAAAGA TTTGCCAGTG
    TTGTTGAGTA ACAAACTTGA AGGGGATACG TTCTTCCTCC TCATTCAGTC TCTGAAAAAT
    AATCTTATTG GAAAAGATCC CTCACTGGTG TATCAGCATC TTTTATATCT GAGTAAAGCA
    GAAAGGTTCA TGATGATGTT GACCCTAATT AGCAAGAGCC AAAAGGAACA AATTGAACAG
    CTCTTTGATG ATCTCTCAGA CACACCAAAT AAACATTTTA CTTTGGAAGA TGTACATGCC
    CTAAAAAGGA AGTATGAGCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973990.1
    CDS77..2920
    Translation

    Target ORF information:

    RefSeq Version XM_014118515.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sperm associated antigen 1 (SPAG1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014118515.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGACTGCCA AAGATTATCC GTCATTTTGG GGCTTTGGAA CAACAAAAAT GTTTAAAATT 
    CCTGTTGAAC ATTTGGATTT CAAGTATATT GAAAAATGTT CAGATGTTAA ACACTTGGAA
    AAAATTTTTT GTGTGCTCAG GTCTGGTGAG GAAGGATATT ATCCGGAACT TACGGAATTT
    TGTGAAAAGC GCCTTAAAGG CTTGGCTCCT GAAAGTAGAG CTTTGAGGAA AGATAAACCT
    CCAGCAACAG CATCCAGTTT TACAGCAGAA GAATGGAGAC ATATTGACAG TGATATAAAG
    AGTTGGGTAT CAGAAATTAA AAAAGAAGAT GAAGTATACT TCCATGAGAC TGAAACATTT
    CCAGCAATGG AAGATAATTT GCCTCCAGTT CGTGGTTCAA ACAGCCGTCT TCATGTTAGT
    AAGGAAAAAT ATTCTAATAG TAGACCAACT AAAAAGAAAA TTCCGAGGGA TTATGCAGAA
    TGGGATAAAT TTGACGTGGA AAAGGAATGT TCAAAAATTG ATGAAGATTA CAAAGAAAAA
    ACTGTAGTAA ACAACAAGTC ATATTTGTCT AAAATTGAGA CAAGAATAGG CACAGCAGGT
    CTAACTGAGA AGGAAAAGAC TTGCCTTGCT ACTCGTGAAA AGGAAAAAGG AAATGAAGCT
    TTCAACTCAG GAGACTATGA AGAGGCTGTG ATGTATTATA CTAGGAGTAT ATCAGTGCTT
    CCCACCGTGG TGGCCTACAA CAATCGGGCT CAAGCAGAAC TCAAACTACA GAACTGGAAC
    AGTGCTTTTT GGGATTGTGA AAAGGTCTTG GAATTAGAAC CTGGAAACAT AAAAGCTCTT
    CTTCGCCGTG CCACTACCTA TAAACATCAA AACAAGCTTC AGGAGGCTCT CGAAGATTTG
    AATAAAGTAC TGAATGTTGA ACCTGATAAT GAATTGGCCA AAAAGACCTT GTTGGAGGTT
    GAGAGAGATA TGAAAAATTC TGAACCTGCA TCTAAGACTC AAACCAAAGG GACGAGGATG
    GTTATCCAGG AAGTAGAAAA TTCAGAGGAT GAAGGTGGAA AGGACAGTGG AAGAACACAC
    AAAGACGGCA GTGGAGTTAA GAAGCAGGGT ACCTTGGGTG CAATCACTGT ATTCAAGGGT
    TGGAAACTCG GTGCGTTCGT GAATGGTCGG TGCCAGGAGG GCACGTATTT TGTGAGGGGC
    ACCTCGCCAC GGACGCCTAG GCCGCCGAGC CGGCTGCAGC CGGGAGCCCC GCCGCGCCAA
    GCGCCATGGG CAATCATCCA GAAGAAGCTG TCGGGCGAGG GCGAGGGCGG CGGCAGCGGC
    CGGAGAGGGG CGCGCCGCGA TGGGGCCGGG GGACGTGGGT CCGAACGGAC AACTGGGACT
    ACTGCTAGCG GCCCCGCGGC CTTGAAGAGC CAGGGCAACG AGCTGTTCAA AAGCGGCCAG
    TTCGCCGAGG CGGCCCTCAA GTACTCGGCG GCGATCGCGC GGCTGGAGCC TGCAGGGAGT
    GGAAGTGCAG ACGATCTAAG TGTCTTATAT GCAAATAGAG CGGCGTGTTA CCTGAAGGAA
    GGAAACTGCG GCGGCTGCAT TCAAGATTGT AACAGGGCTC TGGAACTTCA CCCATTCTCA
    GTCAAACCTC TTCTTCGTCG AGCGATGGCC TATGAAACTC TAGAGCAATA TCAGAAGGCC
    TATGTGGATT ACAAAACCGT GTTGCAGATA GACTGCAAAA TCCAGCTGGC AAATGACAGT
    ATTAACAGAA TAACAAGGAT TTTGATGAAT CTCGATGGGC CAAGCTGGCG GGAGAAGCTA
    TCACCCATTC CTGCCGTGCC CACCTCTGCG CATTTGCGAG CTTGGCAGCT GGCAGTAGAG
    CCGCCACCCA ACCAAGTGGG AGATTCCTGC AGCCGTCCAC AGCCTGGCAT CACAGATGAA
    AAAATGTTTA AAAACCTTAA AGAAGAAGGA AATCAATGTG TAAAGGACAA AAACTATAAA
    GATGCCCTTA GTAAATACAG TGAATGCTTA ACGATTAACA ACAAGGAATG TGCCATTTAT
    ACAAACAGAG CTCTCTGTTA CTTGAAGCTG TGCCAGTTTG AAGAGGCAAA GCAGGACTGC
    GATCGGGCCC TTCAGATAGA CAACGGGAAT GTGAAAGCAT GCTATCGACG AGCCCTTGCT
    CACAAAGGAC TTAAGGATTA TCAGAAAAGT TTAAACGATC TCAATAAAGT TCTCCTACTA
    GATTCAAGTA TTGTTGAGGC AAAGGTGGAA CTGGAAGAGG TAACCAGATT CCTTAACATG
    AAGGATAATA TAGCATCATT CAACAAAGAA AAAGAGAGAA GGAAAATTGA GATTCAAGAG
    GTGAGTGAAG GCCAGGAAGA AGAACCTGAA AGGACCTCGG AGGAGCTCTT CACTCACTGC
    ATGGCCTCTG AGAATGGGGA CACAACTAAT GGGCCACCAG AATGCGATGA AGAACTACCG
    ATTACCAAGC CTAACAATGC CTATGAATTT GGTCAGATTA TAAATGCTAT TAGTATGAGG
    AATGATAAAG AAGCCTGTGC ACACCTTTTA GCCATCACTG AACCAAAAGA TTTGCCAGTG
    TTGTTGAGTA ACAAACTTGA AGGGGATACG TTCTTCCTCC TCATTCAGTC TCTGAAAAAT
    AATCTTATTG GAAAAGATCC CTCACTGGTG TATCAGCATC TTTTATATCT GAGTAAAGCA
    GAAAGGTTCA TGATGATGTT GACCCTAATT AGCAAGAGCC AAAAGGAACA AATTGAACAG
    CTCTTTGATG ATCTCTCAGA CACACCAAAT AAACATTTTA CTTTGGAAGA TGTACATGCC
    CTAAAAAGGA AGTATGAGCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.