SNRPE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SNRPE
Entrez Gene ID	6635
Full Name	small nuclear ribonucleoprotein polypeptide E
Synonyms	HYPT11,SME,Sm-E,snRNP-E
General protein information	Preferred Names small nuclear ribonucleoprotein polypeptide E Names small nuclear ribonucleoprotein E sm protein E
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	The protein encoded by this gene is a core component of U small nuclear ribonucleoproteins, which are key components of the pre-mRNA processing spliceosome. The encoded protein plays a role in the 3' end processing of histone transcripts. This protein is one of the targets in the autoimmune disease systemic lupus erythematosus, and mutations in this gene have been associated with hypotrichosis. Several pseudogenes of this gene have been identified. [provided by RefSeq, Jun 2016].
Disorder MIM:	128260
Disorder Html:	Hypotrichosis 11, 615059 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003094.4	NP_003085.1	small nuclear ribonucleoprotein E isoform 1
NM_003094.3	NP_003085.1	small nuclear ribonucleoprotein E isoform 1
NM_001304464.1	NP_001291393.1	small nuclear ribonucleoprotein E isoform 2
NM_001328637.1	NP_001315566.1	small nuclear ribonucleoprotein E isoform 3
NM_001328638.2	NP_001315567.1	small nuclear ribonucleoprotein E isoform 4
NM_001304464.2	NP_001291393.1	small nuclear ribonucleoprotein E isoform 2
NM_001328638.1	NP_001315567.1	small nuclear ribonucleoprotein E isoform 4
NM_001328637.2	NP_001315566.1	small nuclear ribonucleoprotein E isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	SNRPE	NP_003085.1
Pan troglodytes (chimpanzee)	SNRPE	XP_001158544.1
	SNRPE	XP_001099697.1
Mus musculus (house mouse)	LOC102632439	XP_006500578.1
Rattus norvegicus (Norway rat)	Snrpel1	XP_001064438.1
Canis lupus familiaris (dog)	LOC102153759	XP_005615987.1

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, L?hrmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, L?hrmann R
Nature542(7641)318-323(2017 02)

Molecular architecture of the human U4/U6.U5 tri-snRNP.
Agafonov DE, Kastner B, Dybkov O, Hofele RV, Liu WT, Urlaub H, L?hrmann R, Stark H
Science (New York, N.Y.)351(6280)1416-20(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of SNRPE is associated with highly aggressive breast cancers.
Title: Targeting the deregulated spliceosome core machinery in cancer cells triggers mTOR blockade and autophagy.

Mutations in SNRPE, which encodes a core protein of the spliceosome, cause autosomal-dominant hypotrichosis simplex
Title: Mutations in SNRPE, which encodes a core protein of the spliceosome, cause autosomal-dominant hypotrichosis simplex.

growth arrest by SmE directly correlates with the reduction of cyclin E, CDK2, CDC25C and CDC2 expression, and up-regulation of p27Kip
Title: Spliceosomal protein E regulates neoplastic cell growth by modulating expression of cyclin E/CDK2 and G2/M checkpoint proteins.

In the SME intron, the position of the branchpoint A residue within the region base paired with U12 differs from that in P120 and XTF.
Title: Branchpoint selection in the splicing of U12-dependent introns in vitro.

The following SNRPE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNRPE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29802	NM_003094.4 Latest version!	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu60657	NM_001304464.2 Latest version!	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu101713	NM_001328637.2 Latest version!	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu102019	NM_001328638.2 Latest version!	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu29802	NM_003094.3	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu60657	NM_001304464.1	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu102019	NM_001328638.1	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu101713	NM_001328637.1	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SNRPE ( NM_003094.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003085.1 Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29802
Clone ID Related Accession (Same CDS sequence)	NM_003094.4 , NM_003094.3
Accession Version	NM_003094.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein E isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114402.2 and AC118554.2. On Nov 23, 2018 this sequence version replaced NM_003094.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.22008.1, SRR3476690.1091168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000414487.7/ ENSP00000400591.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTACC GTGGCCAGGG TCAGAAAGTG CAGAAGGTTA TGGTGCAGCC CATCAACCTC 
ATCTTCAGAT ACTTACAAAA TAGATCGCGG ATTCAGGTGT GGCTCTATGA GCAAGTGAAT 
ATGCGGATAG AAGGCTGTAT CATTGGTTTT GATGAGTATA TGAACCTTGT ATTAGATGAT 
GCAGAAGAGA TTCATTCTAA AACAAAGTCA AGAAAACAAC TGGGTCGGAT CATGCTAAAA 
GGAGATAATA TTACTCTGCT ACAAAGTGTC TCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003085.1
CDS	62..340
Translation	MAYRGQGQKVQKVMVQPINLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Target ORF information:

RefSeq Version	NM_003094.4
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003094.4

ORF Insert Sequence:

ATGGCGTACC GTGGCCAGGG TCAGAAAGTG CAGAAGGTTA TGGTGCAGCC CATCAACCTC 
ATCTTCAGAT ACTTACAAAA TAGATCGCGG ATTCAGGTGT GGCTCTATGA GCAAGTGAAT 
ATGCGGATAG AAGGCTGTAT CATTGGTTTT GATGAGTATA TGAACCTTGT ATTAGATGAT 
GCAGAAGAGA TTCATTCTAA AACAAAGTCA AGAAAACAAC TGGGTCGGAT CATGCTAAAA 
GGAGATAATA TTACTCTGCT ACAAAGTGTC TCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPE ( NM_003094.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003085.1 Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29802
Clone ID Related Accession (Same CDS sequence)	NM_003094.4 , NM_003094.3
Accession Version	NM_003094.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein E isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW441956.1, BC090951.1, AC114402.2 and AC118554.2. This sequence is a reference standard in the RefSeqGene project. On Jan 27, 2015 this sequence version replaced NM_003094.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC090951.1, SRR1660805.213995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTACC GTGGCCAGGG TCAGAAAGTG CAGAAGGTTA TGGTGCAGCC CATCAACCTC 
ATCTTCAGAT ACTTACAAAA TAGATCGCGG ATTCAGGTGT GGCTCTATGA GCAAGTGAAT 
ATGCGGATAG AAGGCTGTAT CATTGGTTTT GATGAGTATA TGAACCTTGT ATTAGATGAT 
GCAGAAGAGA TTCATTCTAA AACAAAGTCA AGAAAACAAC TGGGTCGGAT CATGCTAAAA 
GGAGATAATA TTACTCTGCT ACAAAGTGTC TCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003085.1
CDS	76..354
Translation	MAYRGQGQKVQKVMVQPINLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Target ORF information:

RefSeq Version	NM_003094.3
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003094.3

ORF Insert Sequence:

ATGGCGTACC GTGGCCAGGG TCAGAAAGTG CAGAAGGTTA TGGTGCAGCC CATCAACCTC 
ATCTTCAGAT ACTTACAAAA TAGATCGCGG ATTCAGGTGT GGCTCTATGA GCAAGTGAAT 
ATGCGGATAG AAGGCTGTAT CATTGGTTTT GATGAGTATA TGAACCTTGT ATTAGATGAT 
GCAGAAGAGA TTCATTCTAA AACAAAGTCA AGAAAACAAC TGGGTCGGAT CATGCTAAAA 
GGAGATAATA TTACTCTGCT ACAAAGTGTC TCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPE ( NM_001304464.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291393.1 Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu60657
Clone ID Related Accession (Same CDS sequence)	NM_001304464.1 , NM_001304464.2
Accession Version	NM_001304464.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein E isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW441956.1, AK307255.1, BC090951.1, AC114402.2 and AC118554.2. Transcript Variant: This variant (2) uses two alternate splice junctions in the 5' end compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DC370543.1, CD172683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCAGGAC TAGGAGCCAC TGTGGTGCAG AACCTCATCT TCAGATACTT ACAAAATAGA 
TCGCGGATTC AGGTGTGGCT CTATGAGCAA GTGAATATGC GGATAGAAGG CTGTATCATT 
GGTTTTGATG AGTATATGAA CCTTGTATTA GATGATGCAG AAGAGATTCA TTCTAAAACA 
AAGTCAAGAA AACAACTGGG TCGGATCATG CTAAAAGGAG ATAATATTAC TCTGCTACAA 
AGTGTCTCCA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291393.1
CDS	144..398
Translation	MSGLGATVVQNLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Target ORF information:

RefSeq Version	NM_001304464.1
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304464.1

ORF Insert Sequence:

ATGTCAGGAC TAGGAGCCAC TGTGGTGCAG AACCTCATCT TCAGATACTT ACAAAATAGA 
TCGCGGATTC AGGTGTGGCT CTATGAGCAA GTGAATATGC GGATAGAAGG CTGTATCATT 
GGTTTTGATG AGTATATGAA CCTTGTATTA GATGATGCAG AAGAGATTCA TTCTAAAACA 
AAGTCAAGAA AACAACTGGG TCGGATCATG CTAAAAGGAG ATAATATTAC TCTGCTACAA 
AGTGTCTCCA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPE ( NM_001304464.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291393.1 Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu60657
Clone ID Related Accession (Same CDS sequence)	NM_001304464.1 , NM_001304464.2
Accession Version	NM_001304464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein E isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114402.2 and AC118554.2. On May 31, 2019 this sequence version replaced NM_001304464.1. Transcript Variant: This variant (2) uses two alternate splice junctions in the 5' end compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DC370543.1, AK307255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCAGGAC TAGGAGCCAC TGTGGTGCAG AACCTCATCT TCAGATACTT ACAAAATAGA 
TCGCGGATTC AGGTGTGGCT CTATGAGCAA GTGAATATGC GGATAGAAGG CTGTATCATT 
GGTTTTGATG AGTATATGAA CCTTGTATTA GATGATGCAG AAGAGATTCA TTCTAAAACA 
AAGTCAAGAA AACAACTGGG TCGGATCATG CTAAAAGGAG ATAATATTAC TCTGCTACAA 
AGTGTCTCCA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291393.1
CDS	130..384
Translation	MSGLGATVVQNLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Target ORF information:

RefSeq Version	NM_001304464.2
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304464.2

ORF Insert Sequence:

ATGTCAGGAC TAGGAGCCAC TGTGGTGCAG AACCTCATCT TCAGATACTT ACAAAATAGA 
TCGCGGATTC AGGTGTGGCT CTATGAGCAA GTGAATATGC GGATAGAAGG CTGTATCATT 
GGTTTTGATG AGTATATGAA CCTTGTATTA GATGATGCAG AAGAGATTCA TTCTAAAACA 
AAGTCAAGAA AACAACTGGG TCGGATCATG CTAAAAGGAG ATAATATTAC TCTGCTACAA 
AGTGTCTCCA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPE ( NM_001328637.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001315566.1 Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101713
Clone ID Related Accession (Same CDS sequence)	NM_001328637.1 , NM_001328637.2
Accession Version	NM_001328637.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein E isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA344740.1, AK307255.1, BC090951.1, AC114402.2 and AC118554.2. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.573797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGG CAAAAGGAGG GAAGAGAACT TCAGCCACTG TGGTGCAGAA CCTCATCTTC 
AGATACTTAC AAAATAGATC GCGGATTCAG GTGTGGCTCT ATGAGCAAGT GAATATGCGG 
ATAGAAGGCT GTATCATTGG TTTTGATGAG TATATGAACC TTGTATTAGA TGATGCAGAA 
GAGATTCATT CTAAAACAAA GTCAAGAAAA CAACTGGGTC GGATCATGCT AAAAGGAGAT 
AATATTACTC TGCTACAAAG TGTCTCCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315566.1
CDS	73..345
Translation	MDAAKGGKRTSATVVQNLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Target ORF information:

RefSeq Version	NM_001328637.1
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328637.1

ORF Insert Sequence:

ATGGACGCGG CAAAAGGAGG GAAGAGAACT TCAGCCACTG TGGTGCAGAA CCTCATCTTC 
AGATACTTAC AAAATAGATC GCGGATTCAG GTGTGGCTCT ATGAGCAAGT GAATATGCGG 
ATAGAAGGCT GTATCATTGG TTTTGATGAG TATATGAACC TTGTATTAGA TGATGCAGAA 
GAGATTCATT CTAAAACAAA GTCAAGAAAA CAACTGGGTC GGATCATGCT AAAAGGAGAT 
AATATTACTC TGCTACAAAG TGTCTCCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPE ( NM_001328637.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001315566.1 Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101713
Clone ID Related Accession (Same CDS sequence)	NM_001328637.1 , NM_001328637.2
Accession Version	NM_001328637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein E isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114402.2 and AC118554.2. On May 31, 2019 this sequence version replaced NM_001328637.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189658.178549.1, SRR1163655.573797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACGCGG CAAAAGGAGG GAAGAGAACT TCAGCCACTG TGGTGCAGAA CCTCATCTTC 
AGATACTTAC AAAATAGATC GCGGATTCAG GTGTGGCTCT ATGAGCAAGT GAATATGCGG 
ATAGAAGGCT GTATCATTGG TTTTGATGAG TATATGAACC TTGTATTAGA TGATGCAGAA 
GAGATTCATT CTAAAACAAA GTCAAGAAAA CAACTGGGTC GGATCATGCT AAAAGGAGAT 
AATATTACTC TGCTACAAAG TGTCTCCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315566.1
CDS	76..348
Translation	MDAAKGGKRTSATVVQNLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Target ORF information:

RefSeq Version	NM_001328637.2
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328637.2

ORF Insert Sequence:

ATGGACGCGG CAAAAGGAGG GAAGAGAACT TCAGCCACTG TGGTGCAGAA CCTCATCTTC 
AGATACTTAC AAAATAGATC GCGGATTCAG GTGTGGCTCT ATGAGCAAGT GAATATGCGG 
ATAGAAGGCT GTATCATTGG TTTTGATGAG TATATGAACC TTGTATTAGA TGATGCAGAA 
GAGATTCATT CTAAAACAAA GTCAAGAAAA CAACTGGGTC GGATCATGCT AAAAGGAGAT 
AATATTACTC TGCTACAAAG TGTCTCCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPE ( NM_001328638.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001315567.1 Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu102019
Clone ID Related Accession (Same CDS sequence)	NM_001328638.2 , NM_001328638.1
Accession Version	NM_001328638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein E isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114402.2 and AC118554.2. On May 31, 2019 this sequence version replaced NM_001328638.1. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (4) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.497687.1, CN272809.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGGAAT GGACGCGGCA AAAGGAGGGA AGAGAACTTC AGAGATCGCG GATTCAGGTG 
TGGCTCTATG AGCAAGTGAA TATGCGGATA GAAGGCTGTA TCATTGGTTT TGATGAGTAT 
ATGAACCTTG TATTAGATGA TGCAGAAGAG ATTCATTCTA AAACAAAGTC AAGAAAACAA 
CTGGGTCGGA TCATGCTAAA AGGAGATAAT ATTACTCTGC TACAAAGTGT CTCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315567.1
CDS	68..307
Translation	MQEWTRQKEGRELQRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Target ORF information:

RefSeq Version	NM_001328638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328638.2

ORF Insert Sequence:

ATGCAGGAAT GGACGCGGCA AAAGGAGGGA AGAGAACTTC AGAGATCGCG GATTCAGGTG 
TGGCTCTATG AGCAAGTGAA TATGCGGATA GAAGGCTGTA TCATTGGTTT TGATGAGTAT 
ATGAACCTTG TATTAGATGA TGCAGAAGAG ATTCATTCTA AAACAAAGTC AAGAAAACAA 
CTGGGTCGGA TCATGCTAAA AGGAGATAAT ATTACTCTGC TACAAAGTGT CTCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPE ( NM_001328638.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001315567.1 Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu102019
Clone ID Related Accession (Same CDS sequence)	NM_001328638.2 , NM_001328638.1
Accession Version	NM_001328638.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	small nuclear ribonucleoprotein E isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA344740.1, CN272809.1, BC090951.1, AC114402.2 and AC118554.2. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (4) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AA344740.1, SRR1163655.497687.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGGAAT GGACGCGGCA AAAGGAGGGA AGAGAACTTC AGAGATCGCG GATTCAGGTG 
TGGCTCTATG AGCAAGTGAA TATGCGGATA GAAGGCTGTA TCATTGGTTT TGATGAGTAT 
ATGAACCTTG TATTAGATGA TGCAGAAGAG ATTCATTCTA AAACAAAGTC AAGAAAACAA 
CTGGGTCGGA TCATGCTAAA AGGAGATAAT ATTACTCTGC TACAAAGTGT CTCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315567.1
CDS	65..304
Translation	MQEWTRQKEGRELQRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Target ORF information:

RefSeq Version	NM_001328638.1
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide E (SNRPE), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328638.1

ORF Insert Sequence:

ATGCAGGAAT GGACGCGGCA AAAGGAGGGA AGAGAACTTC AGAGATCGCG GATTCAGGTG 
TGGCTCTATG AGCAAGTGAA TATGCGGATA GAAGGCTGTA TCATTGGTTT TGATGAGTAT 
ATGAACCTTG TATTAGATGA TGCAGAAGAG ATTCATTCTA AAACAAAGTC AAGAAAACAA 
CTGGGTCGGA TCATGCTAAA AGGAGATAAT ATTACTCTGC TACAAAGTGT CTCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,L?hrmann R,Stark H

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Nature542(7641)318-323(2017 02)
Bertram K,Agafonov DE,Liu WT,Dybkov O,Will CL,Hartmuth K,Urlaub H,Kastner B,Stark H,L?hrmann R

Molecular architecture of the human U4/U6.U5 tri-snRNP.
Science (New York, N.Y.)351(6280)1416-20(2016 Mar)
Agafonov DE,Kastner B,Dybkov O,Hofele RV,Liu WT,Urlaub H,L?hrmann R,Stark H

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SNRPE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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