SLIT3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLIT3
Entrez Gene ID	6586
Full Name	slit guidance ligand 3
Synonyms	MEGF5,SLIL2,SLIT1,Slit-3,slit2
General protein information	Preferred Names slit guidance ligand 3 Names slit homolog 3 protein multiple EGF-like domains protein 5 multiple epidermal growth factor-like domains protein 5 slit homolog 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q34-q35.1
Summary	The protein encoded by this gene is secreted, likely interacting with roundabout homolog receptors to effect cell migration. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012].
Disorder MIM:	603745

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271946.2	NP_001258875.2	slit homolog 3 protein isoform 1 precursor
NM_001271946.1	NP_001258875.1	slit homolog 3 protein isoform 1 precursor
NM_003062.4	NP_003053.2	slit homolog 3 protein isoform 2 precursor
XM_017009779.1	XP_016865268.1	slit homolog 3 protein isoform X1
NM_003062.3	NP_003053.1	slit homolog 3 protein isoform 2 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SLIT3	NP_001178379.1
Gallus gallus (chicken)	SLIT3	NP_001183971.1
Pan troglodytes (chimpanzee)	SLIT3	XP_003311018.2
Xenopus tropicalis (tropical clawed frog)	slit3	NP_001072738.1
Homo sapiens (human)	SLIT3	NP_001258875.1
Rattus norvegicus (Norway rat)	Slit3	NP_112611.1
Danio rerio (zebrafish)	slit3	NP_571811.2
	LOC703950	XP_002808486.1
Canis lupus familiaris (dog)	SLIT3	XP_005619283.1
Mus musculus (house mouse)	Slit3	NP_035542.2

Gene Ontology
Bibliography

Related articles in PubMed

Function of Slit/Robo signaling in breast cancer.
Gu F, Ma Y, Zhang J, Qin F, Fu L
Frontiers of medicine9(4)431-6(2015 Dec)

Effects of Slit3 silencing on the invasive ability of lung carcinoma A549 cells.
Zhang C, Guo H, Li B, Sui C, Zhang Y, Xia X, Qin Y, Ye L, Xie F, Wang H, Yuan M, Yuan L, Ye J
Oncology reports34(2)952-60(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of Slit3 induces its tumor suppressive effects in Breast cancer.
Title: Function of Slit/Robo signaling in breast cancer.

Results show that silencing of Slit3 promoted proliferation, migration and invasion of A549 cells and induced epithelial-mesenchymal transition suggesting that Slit3 functions as a tumor suppressor in lung carcinoma.
Title: Effects of Slit3 silencing on the invasive ability of lung carcinoma A549 cells.

Estrogen-dependent expression of SLIT3 may play a key role in regulating nerve-vessel interactions within the complex microenvironment of endometriosis lesions.
Title: Estrogen receptor (ER) agonists differentially regulate neuroangiogenesis in peritoneal endometriosis via the repellent factor SLIT3.

SLIT3 is increased with labour, and both amnion and myometrial studies describe a pro-inflammatory effect of SLIT3 in these tissues.
Title: SLIT3 is increased in supracervical human foetal membranes and in labouring myometrium and regulates pro-inflammatory mediators.

High SLIT3 expression is associated with high-grade gliomas.
Title: Experimental validation of 5 in-silico predicted glioma biomarkers.

The following SLIT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLIT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu96024	XM_017009779.1 Latest version!	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu108530	NM_003062.4 Latest version!	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu108532	NM_001271946.2 Latest version!	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu12233	NM_001271946.1 Latest version!	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu20369	NM_003062.3 Latest version!	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLIT3 ( XM_017009779.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865268.1 Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu96024
Clone ID Related Accession (Same CDS sequence)	XM_017009779.1
Accession Version	XM_017009779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	slit homolog 3 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG ACCTGGACAG AAATAATATC ACCAGGATCA CCAAGATGGA CTTCGCTGGG 
CTCAAGAACC TCCGAGTCTT GCATCTGGAA GACAACCAGG TCAGCGTCAT CGAGAGAGGC 
GCCTTCCAGG ACCTGAAGCA GCTAGAGCGA CTGCGCCTGA ACAAGAATAA GCTGCAAGTC 
CTTCCAGAAT TGCTTTTCCA GAGCACGCCG AAGCTCACCA GACTAGATTT GAGTGAAAAC 
CAGATCCAGG GGATCCCGAG GAAGGCGTTC CGCGGCATCA CCGATGTGAA GAACCTGCAA 
CTGGACAACA ACCACATCAG CTGCATTGAA GATGGAGCCT TCCGAGCGCT GCGCGATTTG 
GAGATCCTTA CCCTCAACAA CAACAACATC AGTCGCATCC TGGTCACCAG CTTCAACCAC 
ATGCCGAAGA TCCGAACTCT GCGCCTCCAC TCCAACCACC TGTACTGCGA CTGCCACCTG 
GCCTGGCTCT CGGATTGGCT GCGACAGCGA CGGACAGTTG GCCAGTTCAC ACTCTGCATG 
GCTCCTGTGC ATTTGAGGGG CTTCAACGTG GCGGATGTGC AGAAGAAGGA GTACGTGTGC 
CCAGCCCCCC ACTCGGAGCC CCCATCCTGC AATGCCAACT CCATCTCCTG CCCTTCGCCC 
TGCACGTGCA GCAATAACAT CGTGGACTGT CGAGGAAAGG GCTTGATGGA GATTCCTGCC 
AACTTGCCGG AGGGCATCGT CGAAATACGC CTAGAACAGA ACTCCATCAA AGCCATCCCT 
GCAGGAGCCT TCACCCAGTA CAAGAAACTG AAGCGAATAG ACATCAGCAA GAATCAGATA 
TCGGATATTG CTCCAGATGC CTTCCAGGGC CTGAAATCAC TCACATCGCT GGTCCTGTAT 
GGGAACAAGA TCACCGAGAT TGTCAAGGGA CTGTTTGATG GGCTGGTGTC CCTACAGCTG 
CTCCTCCTCA ATGCCAACAA GATCAACTGC CTGCGGGTGA ACACGTTTCA GGACCTGCAG 
AACCTCAACT TGCTCTCCCT GTATGACAAC AAGCTGCAGA CCATCAGCAA GGGGCTCTTC 
GCCCCTCTGC AGTCCATCCA GACACTCCAC TTAGCCCAAA ACCCATTTGT GTGCGACTGC 
CACTTGAAGT GGCTGGCCGA CTACCTCCAG GACAACCCCA TCGAGACAAG CGGGGCCCGC 
TGCAGCAGCC CGCGCCGACT CGCCAACAAG CGCATCAGCC AGATCAAGAG CAAGAAGTTC 
CGCTGCTCAG GCTCCGAGGA TTACCGCAGC AGGTTCAGCA GCGAGTGCTT CATGGACCTC 
GTGTGCCCCG AGAAGTGTCG CTGTGAGGGC ACGATTGTGG ACTGCTCCAA CCAGAAGCTG 
GTCCGCATCC CAAGCCACCT CCCTGAATAT GTCACCGACC TGCGACTGAA TGACAATGAG 
GTATCTGTTC TGGAGGCCAC TGGCATCTTC AAGAAGTTGC CCAACCTGCG GAAAATAAAT 
CTGAGTAACA ATAAGATCAA GGAGGTGCGA GAGGGAGCTT TCGATGGAGC AGCCAGCGTG 
CAGGAGCTGA TGCTGACAGG GAACCAGCTG GAGACCGTGC ACGGGCGCGT GTTCCGTGGC 
CTCAGTGGCC TCAAAACCTT GATGCTGAGG AGTAACTTGA TCGGCTGTGT GAGTAATGAC 
ACCTTTGCCG GCCTGAGTTC GGTGAGACTG CTGTCCCTCT ATGACAATCG GATCACCACC 
ATCACCCCTG GGGCCTTCAC CACGCTTGTC TCCCTGTCCA CCATAAACCT CCTGTCCAAC 
CCCTTCAACT GCAACTGCCA CCTGGCCTGG CTCGGCAAGT GGTTGAGGAA GAGGCGGATC 
GTCAGTGGGA ACCCTAGGTG CCAGAAGCCA TTTTTCCTCA AGGAGATTCC CATCCAGGAT 
GTGGCCATCC AGGACTTCAC CTGTGATGGC AACGAGGAGA GTAGCTGCCA GCTGAGCCCG 
CGCTGCCCGG AGCAGTGCAC CTGTATGGAG ACAGTGGTGC GATGCAGCAA CAAGGGGCTC 
CGCGCCCTCC CCAGAGGCAT GCCCAAGGAT GTGACCGAGC TGTACCTGGA AGGAAACCAC 
CTAACAGCCG TGCCCAGAGA GCTGTCCGCC CTCCGACACC TGACGCTTAT TGACCTGAGC 
AACAACAGCA TCAGCATGCT GACCAATTAC ACCTTCAGTA ACATGTCTCA CCTCTCCACT 
CTGATCCTGA GCTACAACCG GCTGAGGTGC ATCCCCGTCC ACGCCTTCAA CGGGCTGCGG 
TCCCTGCGAG TGCTAACCCT CCATGGCAAT GACATTTCCA GCGTTCCTGA AGGCTCCTTC 
AACGACCTCA CATCTCTTTC CCATCTGGCG CTGGGAACCA ACCCACTCCA CTGTGACTGC 
AGTCTTCGGT GGCTGTCGGA GTGGGTGAAG GCGGGGTACA AGGAGCCTGG CATCGCCCGC 
TGCAGTAGCC CTGAGCCCAT GGCTGACAGG CTCCTGCTCA CCACCCCAAC CCACCGCTTC 
CAGTGCAAAG GGCCAGTGGA CATCAACATT GTGGCCAAAT GCAATGCCTG CCTCTCCAGC 
CCGTGCAAGA ATAACGGGAC ATGCACCCAG GACCCTGTGG AGCTGTACCG CTGTGCCTGC 
CCCTACAGCT ACAAGGGCAA GGACTGCACT GTGCCCATCA ACACCTGCAT CCAGAACCCC 
TGTCAGCATG GAGGCACCTG CCACCTGAGT GACAGCCACA AGGATGGGTT CAGCTGCTCC 
TGCCCTCTGG GCTTTGAGGG GCAGCGGTGT GAGATCAACC CAGATGACTG TGAGGACAAC 
GACTGCGAAA ACAATGCCAC CTGCGTGGAC GGGATCAACA ACTACGTGTG TATCTGTCCG 
CCTAACTACA CAGGTGAGCT ATGCGACGAG GTGATTGACC ACTGTGTGCC TGAGCTGAAC 
CTCTGTCAGC ATGAGGCCAA GTGCATCCCC CTGGACAAAG GATTCAGCTG CGAGTGTGTC 
CCTGGCTACA GCGGGAAGCT CTGTGAGACA GACAATGATG ACTGTGTGGC CCACAAGTGC 
CGCCACGGGG CCCAGTGCGT GGACACAATC AATGGCTACA CATGCACCTG CCCCCAGGGC 
TTCAGTGGAC CCTTCTGTGA ACACCCCCCA CCCATGGTCC TACTGCAGAC CAGCCCATGC 
GACCAGTACG AGTGCCAGAA CGGGGCCCAG TGCATCGTGG TGCAGCAGGA GCCCACCTGC 
CGCTGCCCAC CAGGCTTCGC CGGCCCCAGA TGCGAGAAGC TCATCACTGT CAACTTCGTG 
GGCAAAGACT CCTACGTGGA ACTGGCCTCC GCCAAGGTCC GACCCCAGGC CAACATCTCC 
CTGCAGGTGG CCACTGACAA GGACAACGGC ATCCTTCTCT ACAAAGGAGA CAATGACCCC 
CTGGCACTGG AGCTGTACCA GGGCCACGTG CGGCTGGTCT ATGACAGCCT GAGTTCCCCT 
CCAACCACAG TGTACAGTGT GGAGACAGTG AATGATGGGC AGTTTCACAG TGTGGAGCTG 
GTGACGCTAA ACCAGACCCT GAACCTAGTA GTGGACAAAG GAACTCCAAA GAGCCTGGGG 
AAGCTCCAGA AGCAGCCAGC AGTGGGCATC AACAGCCCCC TCTACCTTGG AGGCATCCCC 
ACCTCCACCG GCCTCTCTGC CTTGCGCCAG GGCACGGACC GGCCTCTAGG CGGCTTCCAC 
GGATGCATCC ATGAGGTGCG CATCAACAAC GAGCTGCAGG ACTTCAAGGC CCTCCCACCA 
CAGTCCCTGG GGGTGTCACC AGGCTGCAAG TCCTGCACCG TGTGCAAGCA CGGCCTGTGC 
CGCTCCGTGG AGAAGGACAG CGTGGTGTGC GAGTGCCGCC CAGGCTGGAC CGGCCCACTC 
TGCGATCAGG AGGCCCGGGA CCCCTGCCTC GGCCACAGAT GCCACCATGG AAAATGTGTG 
GCAACTGGGA CCTCATACAT GTGCAAGTGT GCCGAGGGCT ATGGAGGGGA CTTGTGTGAC 
AACAAGAATG ACTCTGCCAA TGCCTGCTCA GCCTTCAAGT GTCACCATGG GCAGTGCCAC 
ATCTCAGACC AAGGGGAGCC CTACTGCCTG TGCCAGCCCG GCTTTAGCGG CGAGCACTGC 
CAACAAGAGA ATCCGTGCCT GGGACAAGTA GTCCGAGAGG TGATCCGCCG CCAGAAAGGT 
TATGCATCAT GTGCCACAGC CTCCAAGGTG CCCATCATGG AATGTCGTGG GGGCTGTGGG 
CCCCAGTGCT GCCAGCCCAC CCGCAGCAAG CGGCGGAAAT ACGTCTTCCA GTGCACGGAC 
GGCTCCTCGT TTGTAGAAGA GGTGGAGAGA CACTTAGAGT GCGGCTGCCT CGCGTGTTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865268.1
CDS	13..4395
Translation	MMGDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLDKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLLQTSPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQENPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQCCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGCLACS

Target ORF information:

RefSeq Version	XM_017009779.1
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009779.1

ORF Insert Sequence:

ATGATGGGTG ACCTGGACAG AAATAATATC ACCAGGATCA CCAAGATGGA CTTCGCTGGG 
CTCAAGAACC TCCGAGTCTT GCATCTGGAA GACAACCAGG TCAGCGTCAT CGAGAGAGGC 
GCCTTCCAGG ACCTGAAGCA GCTAGAGCGA CTGCGCCTGA ACAAGAATAA GCTGCAAGTC 
CTTCCAGAAT TGCTTTTCCA GAGCACGCCG AAGCTCACCA GACTAGATTT GAGTGAAAAC 
CAGATCCAGG GGATCCCGAG GAAGGCGTTC CGCGGCATCA CCGATGTGAA GAACCTGCAA 
CTGGACAACA ACCACATCAG CTGCATTGAA GATGGAGCCT TCCGAGCGCT GCGCGATTTG 
GAGATCCTTA CCCTCAACAA CAACAACATC AGTCGCATCC TGGTCACCAG CTTCAACCAC 
ATGCCGAAGA TCCGAACTCT GCGCCTCCAC TCCAACCACC TGTACTGCGA CTGCCACCTG 
GCCTGGCTCT CGGATTGGCT GCGACAGCGA CGGACAGTTG GCCAGTTCAC ACTCTGCATG 
GCTCCTGTGC ATTTGAGGGG CTTCAACGTG GCGGATGTGC AGAAGAAGGA GTACGTGTGC 
CCAGCCCCCC ACTCGGAGCC CCCATCCTGC AATGCCAACT CCATCTCCTG CCCTTCGCCC 
TGCACGTGCA GCAATAACAT CGTGGACTGT CGAGGAAAGG GCTTGATGGA GATTCCTGCC 
AACTTGCCGG AGGGCATCGT CGAAATACGC CTAGAACAGA ACTCCATCAA AGCCATCCCT 
GCAGGAGCCT TCACCCAGTA CAAGAAACTG AAGCGAATAG ACATCAGCAA GAATCAGATA 
TCGGATATTG CTCCAGATGC CTTCCAGGGC CTGAAATCAC TCACATCGCT GGTCCTGTAT 
GGGAACAAGA TCACCGAGAT TGTCAAGGGA CTGTTTGATG GGCTGGTGTC CCTACAGCTG 
CTCCTCCTCA ATGCCAACAA GATCAACTGC CTGCGGGTGA ACACGTTTCA GGACCTGCAG 
AACCTCAACT TGCTCTCCCT GTATGACAAC AAGCTGCAGA CCATCAGCAA GGGGCTCTTC 
GCCCCTCTGC AGTCCATCCA GACACTCCAC TTAGCCCAAA ACCCATTTGT GTGCGACTGC 
CACTTGAAGT GGCTGGCCGA CTACCTCCAG GACAACCCCA TCGAGACAAG CGGGGCCCGC 
TGCAGCAGCC CGCGCCGACT CGCCAACAAG CGCATCAGCC AGATCAAGAG CAAGAAGTTC 
CGCTGCTCAG GCTCCGAGGA TTACCGCAGC AGGTTCAGCA GCGAGTGCTT CATGGACCTC 
GTGTGCCCCG AGAAGTGTCG CTGTGAGGGC ACGATTGTGG ACTGCTCCAA CCAGAAGCTG 
GTCCGCATCC CAAGCCACCT CCCTGAATAT GTCACCGACC TGCGACTGAA TGACAATGAG 
GTATCTGTTC TGGAGGCCAC TGGCATCTTC AAGAAGTTGC CCAACCTGCG GAAAATAAAT 
CTGAGTAACA ATAAGATCAA GGAGGTGCGA GAGGGAGCTT TCGATGGAGC AGCCAGCGTG 
CAGGAGCTGA TGCTGACAGG GAACCAGCTG GAGACCGTGC ACGGGCGCGT GTTCCGTGGC 
CTCAGTGGCC TCAAAACCTT GATGCTGAGG AGTAACTTGA TCGGCTGTGT GAGTAATGAC 
ACCTTTGCCG GCCTGAGTTC GGTGAGACTG CTGTCCCTCT ATGACAATCG GATCACCACC 
ATCACCCCTG GGGCCTTCAC CACGCTTGTC TCCCTGTCCA CCATAAACCT CCTGTCCAAC 
CCCTTCAACT GCAACTGCCA CCTGGCCTGG CTCGGCAAGT GGTTGAGGAA GAGGCGGATC 
GTCAGTGGGA ACCCTAGGTG CCAGAAGCCA TTTTTCCTCA AGGAGATTCC CATCCAGGAT 
GTGGCCATCC AGGACTTCAC CTGTGATGGC AACGAGGAGA GTAGCTGCCA GCTGAGCCCG 
CGCTGCCCGG AGCAGTGCAC CTGTATGGAG ACAGTGGTGC GATGCAGCAA CAAGGGGCTC 
CGCGCCCTCC CCAGAGGCAT GCCCAAGGAT GTGACCGAGC TGTACCTGGA AGGAAACCAC 
CTAACAGCCG TGCCCAGAGA GCTGTCCGCC CTCCGACACC TGACGCTTAT TGACCTGAGC 
AACAACAGCA TCAGCATGCT GACCAATTAC ACCTTCAGTA ACATGTCTCA CCTCTCCACT 
CTGATCCTGA GCTACAACCG GCTGAGGTGC ATCCCCGTCC ACGCCTTCAA CGGGCTGCGG 
TCCCTGCGAG TGCTAACCCT CCATGGCAAT GACATTTCCA GCGTTCCTGA AGGCTCCTTC 
AACGACCTCA CATCTCTTTC CCATCTGGCG CTGGGAACCA ACCCACTCCA CTGTGACTGC 
AGTCTTCGGT GGCTGTCGGA GTGGGTGAAG GCGGGGTACA AGGAGCCTGG CATCGCCCGC 
TGCAGTAGCC CTGAGCCCAT GGCTGACAGG CTCCTGCTCA CCACCCCAAC CCACCGCTTC 
CAGTGCAAAG GGCCAGTGGA CATCAACATT GTGGCCAAAT GCAATGCCTG CCTCTCCAGC 
CCGTGCAAGA ATAACGGGAC ATGCACCCAG GACCCTGTGG AGCTGTACCG CTGTGCCTGC 
CCCTACAGCT ACAAGGGCAA GGACTGCACT GTGCCCATCA ACACCTGCAT CCAGAACCCC 
TGTCAGCATG GAGGCACCTG CCACCTGAGT GACAGCCACA AGGATGGGTT CAGCTGCTCC 
TGCCCTCTGG GCTTTGAGGG GCAGCGGTGT GAGATCAACC CAGATGACTG TGAGGACAAC 
GACTGCGAAA ACAATGCCAC CTGCGTGGAC GGGATCAACA ACTACGTGTG TATCTGTCCG 
CCTAACTACA CAGGTGAGCT ATGCGACGAG GTGATTGACC ACTGTGTGCC TGAGCTGAAC 
CTCTGTCAGC ATGAGGCCAA GTGCATCCCC CTGGACAAAG GATTCAGCTG CGAGTGTGTC 
CCTGGCTACA GCGGGAAGCT CTGTGAGACA GACAATGATG ACTGTGTGGC CCACAAGTGC 
CGCCACGGGG CCCAGTGCGT GGACACAATC AATGGCTACA CATGCACCTG CCCCCAGGGC 
TTCAGTGGAC CCTTCTGTGA ACACCCCCCA CCCATGGTCC TACTGCAGAC CAGCCCATGC 
GACCAGTACG AGTGCCAGAA CGGGGCCCAG TGCATCGTGG TGCAGCAGGA GCCCACCTGC 
CGCTGCCCAC CAGGCTTCGC CGGCCCCAGA TGCGAGAAGC TCATCACTGT CAACTTCGTG 
GGCAAAGACT CCTACGTGGA ACTGGCCTCC GCCAAGGTCC GACCCCAGGC CAACATCTCC 
CTGCAGGTGG CCACTGACAA GGACAACGGC ATCCTTCTCT ACAAAGGAGA CAATGACCCC 
CTGGCACTGG AGCTGTACCA GGGCCACGTG CGGCTGGTCT ATGACAGCCT GAGTTCCCCT 
CCAACCACAG TGTACAGTGT GGAGACAGTG AATGATGGGC AGTTTCACAG TGTGGAGCTG 
GTGACGCTAA ACCAGACCCT GAACCTAGTA GTGGACAAAG GAACTCCAAA GAGCCTGGGG 
AAGCTCCAGA AGCAGCCAGC AGTGGGCATC AACAGCCCCC TCTACCTTGG AGGCATCCCC 
ACCTCCACCG GCCTCTCTGC CTTGCGCCAG GGCACGGACC GGCCTCTAGG CGGCTTCCAC 
GGATGCATCC ATGAGGTGCG CATCAACAAC GAGCTGCAGG ACTTCAAGGC CCTCCCACCA 
CAGTCCCTGG GGGTGTCACC AGGCTGCAAG TCCTGCACCG TGTGCAAGCA CGGCCTGTGC 
CGCTCCGTGG AGAAGGACAG CGTGGTGTGC GAGTGCCGCC CAGGCTGGAC CGGCCCACTC 
TGCGATCAGG AGGCCCGGGA CCCCTGCCTC GGCCACAGAT GCCACCATGG AAAATGTGTG 
GCAACTGGGA CCTCATACAT GTGCAAGTGT GCCGAGGGCT ATGGAGGGGA CTTGTGTGAC 
AACAAGAATG ACTCTGCCAA TGCCTGCTCA GCCTTCAAGT GTCACCATGG GCAGTGCCAC 
ATCTCAGACC AAGGGGAGCC CTACTGCCTG TGCCAGCCCG GCTTTAGCGG CGAGCACTGC 
CAACAAGAGA ATCCGTGCCT GGGACAAGTA GTCCGAGAGG TGATCCGCCG CCAGAAAGGT 
TATGCATCAT GTGCCACAGC CTCCAAGGTG CCCATCATGG AATGTCGTGG GGGCTGTGGG 
CCCCAGTGCT GCCAGCCCAC CCGCAGCAAG CGGCGGAAAT ACGTCTTCCA GTGCACGGAC 
GGCTCCTCGT TTGTAGAAGA GGTGGAGAGA CACTTAGAGT GCGGCTGCCT CGCGTGTTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT3 ( NM_003062.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003053.2 Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu108530
Clone ID Related Accession (Same CDS sequence)	NM_003062.4
Accession Version	NM_003062.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	slit homolog 3 protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008479.6, AC008409.5, AC094081.3, AC027311.4 and AC011365.5. On Jun 2, 2019 this sequence version replaced NM_003062.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146759.1, AB011538.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCCG GGTGGGCAGG GGTCGGCGCC GCCGTGCGCG CCCGCCTGGC GCTGGCCTTG 
GCGCTGGCGA GCGTCCTGAG TGGGCCTCCA GCCGTCGCCT GCCCCACCAA GTGTACCTGC 
TCCGCTGCCA GCGTGGACTG CCACGGGCTG GGCCTCCGCG CGGTTCCTCG GGGCATCCCC 
CGCAACGCTG AGCGCCTTGA CCTGGACAGA AATAATATCA CCAGGATCAC CAAGATGGAC 
TTCGCTGGGC TCAAGAACCT CCGAGTCTTG CATCTGGAAG ACAACCAGGT CAGCGTCATC 
GAGAGAGGCG CCTTCCAGGA CCTGAAGCAG CTAGAGCGAC TGCGCCTGAA CAAGAATAAG 
CTGCAAGTCC TTCCAGAATT GCTTTTCCAG AGCACGCCGA AGCTCACCAG ACTAGATTTG 
AGTGAAAACC AGATCCAGGG GATCCCGAGG AAGGCGTTCC GCGGCATCAC CGATGTGAAG 
AACCTGCAAC TGGACAACAA CCACATCAGC TGCATTGAAG ATGGAGCCTT CCGAGCGCTG 
CGCGATTTGG AGATCCTTAC CCTCAACAAC AACAACATCA GTCGCATCCT GGTCACCAGC 
TTCAACCACA TGCCGAAGAT CCGAACTCTG CGCCTCCACT CCAACCACCT GTACTGCGAC 
TGCCACCTGG CCTGGCTCTC GGATTGGCTG CGACAGCGAC GGACAGTTGG CCAGTTCACA 
CTCTGCATGG CTCCTGTGCA TTTGAGGGGC TTCAACGTGG CGGATGTGCA GAAGAAGGAG 
TACGTGTGCC CAGCCCCCCA CTCGGAGCCC CCATCCTGCA ATGCCAACTC CATCTCCTGC 
CCTTCGCCCT GCACGTGCAG CAATAACATC GTGGACTGTC GAGGAAAGGG CTTGATGGAG 
ATTCCTGCCA ACTTGCCGGA GGGCATCGTC GAAATACGCC TAGAACAGAA CTCCATCAAA 
GCCATCCCTG CAGGAGCCTT CACCCAGTAC AAGAAACTGA AGCGAATAGA CATCAGCAAG 
AATCAGATAT CGGATATTGC TCCAGATGCC TTCCAGGGCC TGAAATCACT CACATCGCTG 
GTCCTGTATG GGAACAAGAT CACCGAGATT GTCAAGGGAC TGTTTGATGG GCTGGTGTCC 
CTACAGCTGC TCCTCCTCAA TGCCAACAAG ATCAACTGCC TGCGGGTGAA CACGTTTCAG 
GACCTGCAGA ACCTCAACTT GCTCTCCCTG TATGACAACA AGCTGCAGAC CATCAGCAAG 
GGGCTCTTCG CCCCTCTGCA GTCCATCCAG ACACTCCACT TAGCCCAAAA CCCATTTGTG 
TGCGACTGCC ACTTGAAGTG GCTGGCCGAC TACCTCCAGG ACAACCCCAT CGAGACAAGC 
GGGGCCCGCT GCAGCAGCCC GCGCCGACTC GCCAACAAGC GCATCAGCCA GATCAAGAGC 
AAGAAGTTCC GCTGCTCAGG CTCCGAGGAT TACCGCAGCA GGTTCAGCAG CGAGTGCTTC 
ATGGACCTCG TGTGCCCCGA GAAGTGTCGC TGTGAGGGCA CGATTGTGGA CTGCTCCAAC 
CAGAAGCTGG TCCGCATCCC AAGCCACCTC CCTGAATATG TCACCGACCT GCGACTGAAT 
GACAATGAGG TATCTGTTCT GGAGGCCACT GGCATCTTCA AGAAGTTGCC CAACCTGCGG 
AAAATAAATC TGAGTAACAA TAAGATCAAG GAGGTGCGAG AGGGAGCTTT CGATGGAGCA 
GCCAGCGTGC AGGAGCTGAT GCTGACAGGG AACCAGCTGG AGACCGTGCA CGGGCGCGTG 
TTCCGTGGCC TCAGTGGCCT CAAAACCTTG ATGCTGAGGA GTAACTTGAT CGGCTGTGTG 
AGTAATGACA CCTTTGCCGG CCTGAGTTCG GTGAGACTGC TGTCCCTCTA TGACAATCGG 
ATCACCACCA TCACCCCTGG GGCCTTCACC ACGCTTGTCT CCCTGTCCAC CATAAACCTC 
CTGTCCAACC CCTTCAACTG CAACTGCCAC CTGGCCTGGC TCGGCAAGTG GTTGAGGAAG 
AGGCGGATCG TCAGTGGGAA CCCTAGGTGC CAGAAGCCAT TTTTCCTCAA GGAGATTCCC 
ATCCAGGATG TGGCCATCCA GGACTTCACC TGTGATGGCA ACGAGGAGAG TAGCTGCCAG 
CTGAGCCCGC GCTGCCCGGA GCAGTGCACC TGTATGGAGA CAGTGGTGCG ATGCAGCAAC 
AAGGGGCTCC GCGCCCTCCC CAGAGGCATG CCCAAGGATG TGACCGAGCT GTACCTGGAA 
GGAAACCACC TAACAGCCGT GCCCAGAGAG CTGTCCGCCC TCCGACACCT GACGCTTATT 
GACCTGAGCA ACAACAGCAT CAGCATGCTG ACCAATTACA CCTTCAGTAA CATGTCTCAC 
CTCTCCACTC TGATCCTGAG CTACAACCGG CTGAGGTGCA TCCCCGTCCA CGCCTTCAAC 
GGGCTGCGGT CCCTGCGAGT GCTAACCCTC CATGGCAATG ACATTTCCAG CGTTCCTGAA 
GGCTCCTTCA ACGACCTCAC ATCTCTTTCC CATCTGGCGC TGGGAACCAA CCCACTCCAC 
TGTGACTGCA GTCTTCGGTG GCTGTCGGAG TGGGTGAAGG CGGGGTACAA GGAGCCTGGC 
ATCGCCCGCT GCAGTAGCCC TGAGCCCATG GCTGACAGGC TCCTGCTCAC CACCCCAACC 
CACCGCTTCC AGTGCAAAGG GCCAGTGGAC ATCAACATTG TGGCCAAATG CAATGCCTGC 
CTCTCCAGCC CGTGCAAGAA TAACGGGACA TGCACCCAGG ACCCTGTGGA GCTGTACCGC 
TGTGCCTGCC CCTACAGCTA CAAGGGCAAG GACTGCACTG TGCCCATCAA CACCTGCATC 
CAGAACCCCT GTCAGCATGG AGGCACCTGC CACCTGAGTG ACAGCCACAA GGATGGGTTC 
AGCTGCTCCT GCCCTCTGGG CTTTGAGGGG CAGCGGTGTG AGATCAACCC AGATGACTGT 
GAGGACAACG ACTGCGAAAA CAATGCCACC TGCGTGGACG GGATCAACAA CTACGTGTGT 
ATCTGTCCGC CTAACTACAC AGGTGAGCTA TGCGACGAGG TGATTGACCA CTGTGTGCCT 
GAGCTGAACC TCTGTCAGCA TGAGGCCAAG TGCATCCCCC TGGACAAAGG ATTCAGCTGC 
GAGTGTGTCC CTGGCTACAG CGGGAAGCTC TGTGAGACAG ACAATGATGA CTGTGTGGCC 
CACAAGTGCC GCCACGGGGC CCAGTGCGTG GACACAATCA ATGGCTACAC ATGCACCTGC 
CCCCAGGGCT TCAGTGGACC CTTCTGTGAA CACCCCCCAC CCATGGTCCT ACTGCAGACC 
AGCCCATGCG ACCAGTACGA GTGCCAGAAC GGGGCCCAGT GCATCGTGGT GCAGCAGGAG 
CCCACCTGCC GCTGCCCACC AGGCTTCGCC GGCCCCAGAT GCGAGAAGCT CATCACTGTC 
AACTTCGTGG GCAAAGACTC CTACGTGGAA CTGGCCTCCG CCAAGGTCCG ACCCCAGGCC 
AACATCTCCC TGCAGGTGGC CACTGACAAG GACAACGGCA TCCTTCTCTA CAAAGGAGAC 
AATGACCCCC TGGCACTGGA GCTGTACCAG GGCCACGTGC GGCTGGTCTA TGACAGCCTG 
AGTTCCCCTC CAACCACAGT GTACAGTGTG GAGACAGTGA ATGATGGGCA GTTTCACAGT 
GTGGAGCTGG TGACGCTAAA CCAGACCCTG AACCTAGTAG TGGACAAAGG AACTCCAAAG 
AGCCTGGGGA AGCTCCAGAA GCAGCCAGCA GTGGGCATCA ACAGCCCCCT CTACCTTGGA 
GGCATCCCCA CCTCCACCGG CCTCTCTGCC TTGCGCCAGG GCACGGACCG GCCTCTAGGC 
GGCTTCCACG GATGCATCCA TGAGGTGCGC ATCAACAACG AGCTGCAGGA CTTCAAGGCC 
CTCCCACCAC AGTCCCTGGG GGTGTCACCA GGCTGCAAGT CCTGCACCGT GTGCAAGCAC 
GGCCTGTGCC GCTCCGTGGA GAAGGACAGC GTGGTGTGCG AGTGCCGCCC AGGCTGGACC 
GGCCCACTCT GCGATCAGGA GGCCCGGGAC CCCTGCCTCG GCCACAGATG CCACCATGGA 
AAATGTGTGG CAACTGGGAC CTCATACATG TGCAAGTGTG CCGAGGGCTA TGGAGGGGAC 
TTGTGTGACA ACAAGAATGA CTCTGCCAAT GCCTGCTCAG CCTTCAAGTG TCACCATGGG 
CAGTGCCACA TCTCAGACCA AGGGGAGCCC TACTGCCTGT GCCAGCCCGG CTTTAGCGGC 
GAGCACTGCC AACAAGAGAA TCCGTGCCTG GGACAAGTAG TCCGAGAGGT GATCCGCCGC 
CAGAAAGGTT ATGCATCATG TGCCACAGCC TCCAAGGTGC CCATCATGGA ATGTCGTGGG 
GGCTGTGGGC CCCAGTGCTG CCAGCCCACC CGCAGCAAGC GGCGGAAATA CGTCTTCCAG 
TGCACGGACG GCTCCTCGTT TGTAGAAGAG GTGGAGAGAC ACTTAGAGTG CGGCTGCCTC 
GCGTGTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003053.2
CDS	431..5002
Translation	MAPGWAGVGAAVRARLALALALASVLSGPPAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLDKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLLQTSPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQENPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQCCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGCLACS

Target ORF information:

RefSeq Version	NM_003062.4
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003062.4

ORF Insert Sequence:

ATGGCCCCCG GGTGGGCAGG GGTCGGCGCC GCCGTGCGCG CCCGCCTGGC GCTGGCCTTG 
GCGCTGGCGA GCGTCCTGAG TGGGCCTCCA GCCGTCGCCT GCCCCACCAA GTGTACCTGC 
TCCGCTGCCA GCGTGGACTG CCACGGGCTG GGCCTCCGCG CGGTTCCTCG GGGCATCCCC 
CGCAACGCTG AGCGCCTTGA CCTGGACAGA AATAATATCA CCAGGATCAC CAAGATGGAC 
TTCGCTGGGC TCAAGAACCT CCGAGTCTTG CATCTGGAAG ACAACCAGGT CAGCGTCATC 
GAGAGAGGCG CCTTCCAGGA CCTGAAGCAG CTAGAGCGAC TGCGCCTGAA CAAGAATAAG 
CTGCAAGTCC TTCCAGAATT GCTTTTCCAG AGCACGCCGA AGCTCACCAG ACTAGATTTG 
AGTGAAAACC AGATCCAGGG GATCCCGAGG AAGGCGTTCC GCGGCATCAC CGATGTGAAG 
AACCTGCAAC TGGACAACAA CCACATCAGC TGCATTGAAG ATGGAGCCTT CCGAGCGCTG 
CGCGATTTGG AGATCCTTAC CCTCAACAAC AACAACATCA GTCGCATCCT GGTCACCAGC 
TTCAACCACA TGCCGAAGAT CCGAACTCTG CGCCTCCACT CCAACCACCT GTACTGCGAC 
TGCCACCTGG CCTGGCTCTC GGATTGGCTG CGACAGCGAC GGACAGTTGG CCAGTTCACA 
CTCTGCATGG CTCCTGTGCA TTTGAGGGGC TTCAACGTGG CGGATGTGCA GAAGAAGGAG 
TACGTGTGCC CAGCCCCCCA CTCGGAGCCC CCATCCTGCA ATGCCAACTC CATCTCCTGC 
CCTTCGCCCT GCACGTGCAG CAATAACATC GTGGACTGTC GAGGAAAGGG CTTGATGGAG 
ATTCCTGCCA ACTTGCCGGA GGGCATCGTC GAAATACGCC TAGAACAGAA CTCCATCAAA 
GCCATCCCTG CAGGAGCCTT CACCCAGTAC AAGAAACTGA AGCGAATAGA CATCAGCAAG 
AATCAGATAT CGGATATTGC TCCAGATGCC TTCCAGGGCC TGAAATCACT CACATCGCTG 
GTCCTGTATG GGAACAAGAT CACCGAGATT GTCAAGGGAC TGTTTGATGG GCTGGTGTCC 
CTACAGCTGC TCCTCCTCAA TGCCAACAAG ATCAACTGCC TGCGGGTGAA CACGTTTCAG 
GACCTGCAGA ACCTCAACTT GCTCTCCCTG TATGACAACA AGCTGCAGAC CATCAGCAAG 
GGGCTCTTCG CCCCTCTGCA GTCCATCCAG ACACTCCACT TAGCCCAAAA CCCATTTGTG 
TGCGACTGCC ACTTGAAGTG GCTGGCCGAC TACCTCCAGG ACAACCCCAT CGAGACAAGC 
GGGGCCCGCT GCAGCAGCCC GCGCCGACTC GCCAACAAGC GCATCAGCCA GATCAAGAGC 
AAGAAGTTCC GCTGCTCAGG CTCCGAGGAT TACCGCAGCA GGTTCAGCAG CGAGTGCTTC 
ATGGACCTCG TGTGCCCCGA GAAGTGTCGC TGTGAGGGCA CGATTGTGGA CTGCTCCAAC 
CAGAAGCTGG TCCGCATCCC AAGCCACCTC CCTGAATATG TCACCGACCT GCGACTGAAT 
GACAATGAGG TATCTGTTCT GGAGGCCACT GGCATCTTCA AGAAGTTGCC CAACCTGCGG 
AAAATAAATC TGAGTAACAA TAAGATCAAG GAGGTGCGAG AGGGAGCTTT CGATGGAGCA 
GCCAGCGTGC AGGAGCTGAT GCTGACAGGG AACCAGCTGG AGACCGTGCA CGGGCGCGTG 
TTCCGTGGCC TCAGTGGCCT CAAAACCTTG ATGCTGAGGA GTAACTTGAT CGGCTGTGTG 
AGTAATGACA CCTTTGCCGG CCTGAGTTCG GTGAGACTGC TGTCCCTCTA TGACAATCGG 
ATCACCACCA TCACCCCTGG GGCCTTCACC ACGCTTGTCT CCCTGTCCAC CATAAACCTC 
CTGTCCAACC CCTTCAACTG CAACTGCCAC CTGGCCTGGC TCGGCAAGTG GTTGAGGAAG 
AGGCGGATCG TCAGTGGGAA CCCTAGGTGC CAGAAGCCAT TTTTCCTCAA GGAGATTCCC 
ATCCAGGATG TGGCCATCCA GGACTTCACC TGTGATGGCA ACGAGGAGAG TAGCTGCCAG 
CTGAGCCCGC GCTGCCCGGA GCAGTGCACC TGTATGGAGA CAGTGGTGCG ATGCAGCAAC 
AAGGGGCTCC GCGCCCTCCC CAGAGGCATG CCCAAGGATG TGACCGAGCT GTACCTGGAA 
GGAAACCACC TAACAGCCGT GCCCAGAGAG CTGTCCGCCC TCCGACACCT GACGCTTATT 
GACCTGAGCA ACAACAGCAT CAGCATGCTG ACCAATTACA CCTTCAGTAA CATGTCTCAC 
CTCTCCACTC TGATCCTGAG CTACAACCGG CTGAGGTGCA TCCCCGTCCA CGCCTTCAAC 
GGGCTGCGGT CCCTGCGAGT GCTAACCCTC CATGGCAATG ACATTTCCAG CGTTCCTGAA 
GGCTCCTTCA ACGACCTCAC ATCTCTTTCC CATCTGGCGC TGGGAACCAA CCCACTCCAC 
TGTGACTGCA GTCTTCGGTG GCTGTCGGAG TGGGTGAAGG CGGGGTACAA GGAGCCTGGC 
ATCGCCCGCT GCAGTAGCCC TGAGCCCATG GCTGACAGGC TCCTGCTCAC CACCCCAACC 
CACCGCTTCC AGTGCAAAGG GCCAGTGGAC ATCAACATTG TGGCCAAATG CAATGCCTGC 
CTCTCCAGCC CGTGCAAGAA TAACGGGACA TGCACCCAGG ACCCTGTGGA GCTGTACCGC 
TGTGCCTGCC CCTACAGCTA CAAGGGCAAG GACTGCACTG TGCCCATCAA CACCTGCATC 
CAGAACCCCT GTCAGCATGG AGGCACCTGC CACCTGAGTG ACAGCCACAA GGATGGGTTC 
AGCTGCTCCT GCCCTCTGGG CTTTGAGGGG CAGCGGTGTG AGATCAACCC AGATGACTGT 
GAGGACAACG ACTGCGAAAA CAATGCCACC TGCGTGGACG GGATCAACAA CTACGTGTGT 
ATCTGTCCGC CTAACTACAC AGGTGAGCTA TGCGACGAGG TGATTGACCA CTGTGTGCCT 
GAGCTGAACC TCTGTCAGCA TGAGGCCAAG TGCATCCCCC TGGACAAAGG ATTCAGCTGC 
GAGTGTGTCC CTGGCTACAG CGGGAAGCTC TGTGAGACAG ACAATGATGA CTGTGTGGCC 
CACAAGTGCC GCCACGGGGC CCAGTGCGTG GACACAATCA ATGGCTACAC ATGCACCTGC 
CCCCAGGGCT TCAGTGGACC CTTCTGTGAA CACCCCCCAC CCATGGTCCT ACTGCAGACC 
AGCCCATGCG ACCAGTACGA GTGCCAGAAC GGGGCCCAGT GCATCGTGGT GCAGCAGGAG 
CCCACCTGCC GCTGCCCACC AGGCTTCGCC GGCCCCAGAT GCGAGAAGCT CATCACTGTC 
AACTTCGTGG GCAAAGACTC CTACGTGGAA CTGGCCTCCG CCAAGGTCCG ACCCCAGGCC 
AACATCTCCC TGCAGGTGGC CACTGACAAG GACAACGGCA TCCTTCTCTA CAAAGGAGAC 
AATGACCCCC TGGCACTGGA GCTGTACCAG GGCCACGTGC GGCTGGTCTA TGACAGCCTG 
AGTTCCCCTC CAACCACAGT GTACAGTGTG GAGACAGTGA ATGATGGGCA GTTTCACAGT 
GTGGAGCTGG TGACGCTAAA CCAGACCCTG AACCTAGTAG TGGACAAAGG AACTCCAAAG 
AGCCTGGGGA AGCTCCAGAA GCAGCCAGCA GTGGGCATCA ACAGCCCCCT CTACCTTGGA 
GGCATCCCCA CCTCCACCGG CCTCTCTGCC TTGCGCCAGG GCACGGACCG GCCTCTAGGC 
GGCTTCCACG GATGCATCCA TGAGGTGCGC ATCAACAACG AGCTGCAGGA CTTCAAGGCC 
CTCCCACCAC AGTCCCTGGG GGTGTCACCA GGCTGCAAGT CCTGCACCGT GTGCAAGCAC 
GGCCTGTGCC GCTCCGTGGA GAAGGACAGC GTGGTGTGCG AGTGCCGCCC AGGCTGGACC 
GGCCCACTCT GCGATCAGGA GGCCCGGGAC CCCTGCCTCG GCCACAGATG CCACCATGGA 
AAATGTGTGG CAACTGGGAC CTCATACATG TGCAAGTGTG CCGAGGGCTA TGGAGGGGAC 
TTGTGTGACA ACAAGAATGA CTCTGCCAAT GCCTGCTCAG CCTTCAAGTG TCACCATGGG 
CAGTGCCACA TCTCAGACCA AGGGGAGCCC TACTGCCTGT GCCAGCCCGG CTTTAGCGGC 
GAGCACTGCC AACAAGAGAA TCCGTGCCTG GGACAAGTAG TCCGAGAGGT GATCCGCCGC 
CAGAAAGGTT ATGCATCATG TGCCACAGCC TCCAAGGTGC CCATCATGGA ATGTCGTGGG 
GGCTGTGGGC CCCAGTGCTG CCAGCCCACC CGCAGCAAGC GGCGGAAATA CGTCTTCCAG 
TGCACGGACG GCTCCTCGTT TGTAGAAGAG GTGGAGAGAC ACTTAGAGTG CGGCTGCCTC 
GCGTGTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT3 ( NM_001271946.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258875.2 Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 1, mRNA.

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CloneID	OHu108532
Clone ID Related Accession (Same CDS sequence)	NM_001271946.2
Accession Version	NM_001271946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	slit homolog 3 protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008479.6, AC008409.5, AC094081.3, AC027311.4 and AC011365.5. On Jun 2, 2019 this sequence version replaced NM_001271946.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC142717.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCCG GGTGGGCAGG GGTCGGCGCC GCCGTGCGCG CCCGCCTGGC GCTGGCCTTG 
GCGCTGGCGA GCGTCCTGAG TGGGCCTCCA GCCGTCGCCT GCCCCACCAA GTGTACCTGC 
TCCGCTGCCA GCGTGGACTG CCACGGGCTG GGCCTCCGCG CGGTTCCTCG GGGCATCCCC 
CGCAACGCTG AGCGCCTTGA CCTGGACAGA AATAATATCA CCAGGATCAC CAAGATGGAC 
TTCGCTGGGC TCAAGAACCT CCGAGTCTTG CATCTGGAAG ACAACCAGGT CAGCGTCATC 
GAGAGAGGCG CCTTCCAGGA CCTGAAGCAG CTAGAGCGAC TGCGCCTGAA CAAGAATAAG 
CTGCAAGTCC TTCCAGAATT GCTTTTCCAG AGCACGCCGA AGCTCACCAG ACTAGATTTG 
AGTGAAAACC AGATCCAGGG GATCCCGAGG AAGGCGTTCC GCGGCATCAC CGATGTGAAG 
AACCTGCAAC TGGACAACAA CCACATCAGC TGCATTGAAG ATGGAGCCTT CCGAGCGCTG 
CGCGATTTGG AGATCCTTAC CCTCAACAAC AACAACATCA GTCGCATCCT GGTCACCAGC 
TTCAACCACA TGCCGAAGAT CCGAACTCTG CGCCTCCACT CCAACCACCT GTACTGCGAC 
TGCCACCTGG CCTGGCTCTC GGATTGGCTG CGACAGCGAC GGACAGTTGG CCAGTTCACA 
CTCTGCATGG CTCCTGTGCA TTTGAGGGGC TTCAACGTGG CGGATGTGCA GAAGAAGGAG 
TACGTGTGCC CAGCCCCCCA CTCGGAGCCC CCATCCTGCA ATGCCAACTC CATCTCCTGC 
CCTTCGCCCT GCACGTGCAG CAATAACATC GTGGACTGTC GAGGAAAGGG CTTGATGGAG 
ATTCCTGCCA ACTTGCCGGA GGGCATCGTC GAAATACGCC TAGAACAGAA CTCCATCAAA 
GCCATCCCTG CAGGAGCCTT CACCCAGTAC AAGAAACTGA AGCGAATAGA CATCAGCAAG 
AATCAGATAT CGGATATTGC TCCAGATGCC TTCCAGGGCC TGAAATCACT CACATCGCTG 
GTCCTGTATG GGAACAAGAT CACCGAGATT GTCAAGGGAC TGTTTGATGG GCTGGTGTCC 
CTACAGCTGC TCCTCCTCAA TGCCAACAAG ATCAACTGCC TGCGGGTGAA CACGTTTCAG 
GACCTGCAGA ACCTCAACTT GCTCTCCCTG TATGACAACA AGCTGCAGAC CATCAGCAAG 
GGGCTCTTCG CCCCTCTGCA GTCCATCCAG ACACTCCACT TAGCCCAAAA CCCATTTGTG 
TGCGACTGCC ACTTGAAGTG GCTGGCCGAC TACCTCCAGG ACAACCCCAT CGAGACAAGC 
GGGGCCCGCT GCAGCAGCCC GCGCCGACTC GCCAACAAGC GCATCAGCCA GATCAAGAGC 
AAGAAGTTCC GCTGCTCAGG CTCCGAGGAT TACCGCAGCA GGTTCAGCAG CGAGTGCTTC 
ATGGACCTCG TGTGCCCCGA GAAGTGTCGC TGTGAGGGCA CGATTGTGGA CTGCTCCAAC 
CAGAAGCTGG TCCGCATCCC AAGCCACCTC CCTGAATATG TCACCGACCT GCGACTGAAT 
GACAATGAGG TATCTGTTCT GGAGGCCACT GGCATCTTCA AGAAGTTGCC CAACCTGCGG 
AAAATAAATC TGAGTAACAA TAAGATCAAG GAGGTGCGAG AGGGAGCTTT CGATGGAGCA 
GCCAGCGTGC AGGAGCTGAT GCTGACAGGG AACCAGCTGG AGACCGTGCA CGGGCGCGTG 
TTCCGTGGCC TCAGTGGCCT CAAAACCTTG ATGCTGAGGA GTAACTTGAT CGGCTGTGTG 
AGTAATGACA CCTTTGCCGG CCTGAGTTCG GTGAGACTGC TGTCCCTCTA TGACAATCGG 
ATCACCACCA TCACCCCTGG GGCCTTCACC ACGCTTGTCT CCCTGTCCAC CATAAACCTC 
CTGTCCAACC CCTTCAACTG CAACTGCCAC CTGGCCTGGC TCGGCAAGTG GTTGAGGAAG 
AGGCGGATCG TCAGTGGGAA CCCTAGGTGC CAGAAGCCAT TTTTCCTCAA GGAGATTCCC 
ATCCAGGATG TGGCCATCCA GGACTTCACC TGTGATGGCA ACGAGGAGAG TAGCTGCCAG 
CTGAGCCCGC GCTGCCCGGA GCAGTGCACC TGTATGGAGA CAGTGGTGCG ATGCAGCAAC 
AAGGGGCTCC GCGCCCTCCC CAGAGGCATG CCCAAGGATG TGACCGAGCT GTACCTGGAA 
GGAAACCACC TAACAGCCGT GCCCAGAGAG CTGTCCGCCC TCCGACACCT GACGCTTATT 
GACCTGAGCA ACAACAGCAT CAGCATGCTG ACCAATTACA CCTTCAGTAA CATGTCTCAC 
CTCTCCACTC TGATCCTGAG CTACAACCGG CTGAGGTGCA TCCCCGTCCA CGCCTTCAAC 
GGGCTGCGGT CCCTGCGAGT GCTAACCCTC CATGGCAATG ACATTTCCAG CGTTCCTGAA 
GGCTCCTTCA ACGACCTCAC ATCTCTTTCC CATCTGGCGC TGGGAACCAA CCCACTCCAC 
TGTGACTGCA GTCTTCGGTG GCTGTCGGAG TGGGTGAAGG CGGGGTACAA GGAGCCTGGC 
ATCGCCCGCT GCAGTAGCCC TGAGCCCATG GCTGACAGGC TCCTGCTCAC CACCCCAACC 
CACCGCTTCC AGTGCAAAGT ACTCTGGTTT TGCTGCCCAG GGCCAGTGGA CATCAACATT 
GTGGCCAAAT GCAATGCCTG CCTCTCCAGC CCGTGCAAGA ATAACGGGAC ATGCACCCAG 
GACCCTGTGG AGCTGTACCG CTGTGCCTGC CCCTACAGCT ACAAGGGCAA GGACTGCACT 
GTGCCCATCA ACACCTGCAT CCAGAACCCC TGTCAGCATG GAGGCACCTG CCACCTGAGT 
GACAGCCACA AGGATGGGTT CAGCTGCTCC TGCCCTCTGG GCTTTGAGGG GCAGCGGTGT 
GAGATCAACC CAGATGACTG TGAGGACAAC GACTGCGAAA ACAATGCCAC CTGCGTGGAC 
GGGATCAACA ACTACGTGTG TATCTGTCCG CCTAACTACA CAGGTGAGCT ATGCGACGAG 
GTGATTGACC ACTGTGTGCC TGAGCTGAAC CTCTGTCAGC ATGAGGCCAA GTGCATCCCC 
CTGGACAAAG GATTCAGCTG CGAGTGTGTC CCTGGCTACA GCGGGAAGCT CTGTGAGACA 
GACAATGATG ACTGTGTGGC CCACAAGTGC CGCCACGGGG CCCAGTGCGT GGACACAATC 
AATGGCTACA CATGCACCTG CCCCCAGGGC TTCAGTGGAC CCTTCTGTGA ACACCCCCCA 
CCCATGGTCC TACTGCAGAC CAGCCCATGC GACCAGTACG AGTGCCAGAA CGGGGCCCAG 
TGCATCGTGG TGCAGCAGGA GCCCACCTGC CGCTGCCCAC CAGGCTTCGC CGGCCCCAGA 
TGCGAGAAGC TCATCACTGT CAACTTCGTG GGCAAAGACT CCTACGTGGA ACTGGCCTCC 
GCCAAGGTCC GACCCCAGGC CAACATCTCC CTGCAGGTGG CCACTGACAA GGACAACGGC 
ATCCTTCTCT ACAAAGGAGA CAATGACCCC CTGGCACTGG AGCTGTACCA GGGCCACGTG 
CGGCTGGTCT ATGACAGCCT GAGTTCCCCT CCAACCACAG TGTACAGTGT GGAGACAGTG 
AATGATGGGC AGTTTCACAG TGTGGAGCTG GTGACGCTAA ACCAGACCCT GAACCTAGTA 
GTGGACAAAG GAACTCCAAA GAGCCTGGGG AAGCTCCAGA AGCAGCCAGC AGTGGGCATC 
AACAGCCCCC TCTACCTTGG AGGCATCCCC ACCTCCACCG GCCTCTCTGC CTTGCGCCAG 
GGCACGGACC GGCCTCTAGG CGGCTTCCAC GGATGCATCC ATGAGGTGCG CATCAACAAC 
GAGCTGCAGG ACTTCAAGGC CCTCCCACCA CAGTCCCTGG GGGTGTCACC AGGCTGCAAG 
TCCTGCACCG TGTGCAAGCA CGGCCTGTGC CGCTCCGTGG AGAAGGACAG CGTGGTGTGC 
GAGTGCCGCC CAGGCTGGAC CGGCCCACTC TGCGATCAGG AGGCCCGGGA CCCCTGCCTC 
GGCCACAGAT GCCACCATGG AAAATGTGTG GCAACTGGGA CCTCATACAT GTGCAAGTGT 
GCCGAGGGCT ATGGAGGGGA CTTGTGTGAC AACAAGAATG ACTCTGCCAA TGCCTGCTCA 
GCCTTCAAGT GTCACCATGG GCAGTGCCAC ATCTCAGACC AAGGGGAGCC CTACTGCCTG 
TGCCAGCCCG GCTTTAGCGG CGAGCACTGC CAACAAGAGA ATCCGTGCCT GGGACAAGTA 
GTCCGAGAGG TGATCCGCCG CCAGAAAGGT TATGCATCAT GTGCCACAGC CTCCAAGGTG 
CCCATCATGG AATGTCGTGG GGGCTGTGGG CCCCAGTGCT GCCAGCCCAC CCGCAGCAAG 
CGGCGGAAAT ACGTCTTCCA GTGCACGGAC GGCTCCTCGT TTGTAGAAGA GGTGGAGAGA 
CACTTAGAGT GCGGCTGCCT CGCGTGTTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258875.2
CDS	431..5023
Translation	MAPGWAGVGAAVRARLALALALASVLSGPPAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKVLWFCCPGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLDKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLLQTSPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQENPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQCCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGCLACS

Target ORF information:

RefSeq Version	NM_001271946.2
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271946.2

ORF Insert Sequence:

ATGGCCCCCG GGTGGGCAGG GGTCGGCGCC GCCGTGCGCG CCCGCCTGGC GCTGGCCTTG 
GCGCTGGCGA GCGTCCTGAG TGGGCCTCCA GCCGTCGCCT GCCCCACCAA GTGTACCTGC 
TCCGCTGCCA GCGTGGACTG CCACGGGCTG GGCCTCCGCG CGGTTCCTCG GGGCATCCCC 
CGCAACGCTG AGCGCCTTGA CCTGGACAGA AATAATATCA CCAGGATCAC CAAGATGGAC 
TTCGCTGGGC TCAAGAACCT CCGAGTCTTG CATCTGGAAG ACAACCAGGT CAGCGTCATC 
GAGAGAGGCG CCTTCCAGGA CCTGAAGCAG CTAGAGCGAC TGCGCCTGAA CAAGAATAAG 
CTGCAAGTCC TTCCAGAATT GCTTTTCCAG AGCACGCCGA AGCTCACCAG ACTAGATTTG 
AGTGAAAACC AGATCCAGGG GATCCCGAGG AAGGCGTTCC GCGGCATCAC CGATGTGAAG 
AACCTGCAAC TGGACAACAA CCACATCAGC TGCATTGAAG ATGGAGCCTT CCGAGCGCTG 
CGCGATTTGG AGATCCTTAC CCTCAACAAC AACAACATCA GTCGCATCCT GGTCACCAGC 
TTCAACCACA TGCCGAAGAT CCGAACTCTG CGCCTCCACT CCAACCACCT GTACTGCGAC 
TGCCACCTGG CCTGGCTCTC GGATTGGCTG CGACAGCGAC GGACAGTTGG CCAGTTCACA 
CTCTGCATGG CTCCTGTGCA TTTGAGGGGC TTCAACGTGG CGGATGTGCA GAAGAAGGAG 
TACGTGTGCC CAGCCCCCCA CTCGGAGCCC CCATCCTGCA ATGCCAACTC CATCTCCTGC 
CCTTCGCCCT GCACGTGCAG CAATAACATC GTGGACTGTC GAGGAAAGGG CTTGATGGAG 
ATTCCTGCCA ACTTGCCGGA GGGCATCGTC GAAATACGCC TAGAACAGAA CTCCATCAAA 
GCCATCCCTG CAGGAGCCTT CACCCAGTAC AAGAAACTGA AGCGAATAGA CATCAGCAAG 
AATCAGATAT CGGATATTGC TCCAGATGCC TTCCAGGGCC TGAAATCACT CACATCGCTG 
GTCCTGTATG GGAACAAGAT CACCGAGATT GTCAAGGGAC TGTTTGATGG GCTGGTGTCC 
CTACAGCTGC TCCTCCTCAA TGCCAACAAG ATCAACTGCC TGCGGGTGAA CACGTTTCAG 
GACCTGCAGA ACCTCAACTT GCTCTCCCTG TATGACAACA AGCTGCAGAC CATCAGCAAG 
GGGCTCTTCG CCCCTCTGCA GTCCATCCAG ACACTCCACT TAGCCCAAAA CCCATTTGTG 
TGCGACTGCC ACTTGAAGTG GCTGGCCGAC TACCTCCAGG ACAACCCCAT CGAGACAAGC 
GGGGCCCGCT GCAGCAGCCC GCGCCGACTC GCCAACAAGC GCATCAGCCA GATCAAGAGC 
AAGAAGTTCC GCTGCTCAGG CTCCGAGGAT TACCGCAGCA GGTTCAGCAG CGAGTGCTTC 
ATGGACCTCG TGTGCCCCGA GAAGTGTCGC TGTGAGGGCA CGATTGTGGA CTGCTCCAAC 
CAGAAGCTGG TCCGCATCCC AAGCCACCTC CCTGAATATG TCACCGACCT GCGACTGAAT 
GACAATGAGG TATCTGTTCT GGAGGCCACT GGCATCTTCA AGAAGTTGCC CAACCTGCGG 
AAAATAAATC TGAGTAACAA TAAGATCAAG GAGGTGCGAG AGGGAGCTTT CGATGGAGCA 
GCCAGCGTGC AGGAGCTGAT GCTGACAGGG AACCAGCTGG AGACCGTGCA CGGGCGCGTG 
TTCCGTGGCC TCAGTGGCCT CAAAACCTTG ATGCTGAGGA GTAACTTGAT CGGCTGTGTG 
AGTAATGACA CCTTTGCCGG CCTGAGTTCG GTGAGACTGC TGTCCCTCTA TGACAATCGG 
ATCACCACCA TCACCCCTGG GGCCTTCACC ACGCTTGTCT CCCTGTCCAC CATAAACCTC 
CTGTCCAACC CCTTCAACTG CAACTGCCAC CTGGCCTGGC TCGGCAAGTG GTTGAGGAAG 
AGGCGGATCG TCAGTGGGAA CCCTAGGTGC CAGAAGCCAT TTTTCCTCAA GGAGATTCCC 
ATCCAGGATG TGGCCATCCA GGACTTCACC TGTGATGGCA ACGAGGAGAG TAGCTGCCAG 
CTGAGCCCGC GCTGCCCGGA GCAGTGCACC TGTATGGAGA CAGTGGTGCG ATGCAGCAAC 
AAGGGGCTCC GCGCCCTCCC CAGAGGCATG CCCAAGGATG TGACCGAGCT GTACCTGGAA 
GGAAACCACC TAACAGCCGT GCCCAGAGAG CTGTCCGCCC TCCGACACCT GACGCTTATT 
GACCTGAGCA ACAACAGCAT CAGCATGCTG ACCAATTACA CCTTCAGTAA CATGTCTCAC 
CTCTCCACTC TGATCCTGAG CTACAACCGG CTGAGGTGCA TCCCCGTCCA CGCCTTCAAC 
GGGCTGCGGT CCCTGCGAGT GCTAACCCTC CATGGCAATG ACATTTCCAG CGTTCCTGAA 
GGCTCCTTCA ACGACCTCAC ATCTCTTTCC CATCTGGCGC TGGGAACCAA CCCACTCCAC 
TGTGACTGCA GTCTTCGGTG GCTGTCGGAG TGGGTGAAGG CGGGGTACAA GGAGCCTGGC 
ATCGCCCGCT GCAGTAGCCC TGAGCCCATG GCTGACAGGC TCCTGCTCAC CACCCCAACC 
CACCGCTTCC AGTGCAAAGT ACTCTGGTTT TGCTGCCCAG GGCCAGTGGA CATCAACATT 
GTGGCCAAAT GCAATGCCTG CCTCTCCAGC CCGTGCAAGA ATAACGGGAC ATGCACCCAG 
GACCCTGTGG AGCTGTACCG CTGTGCCTGC CCCTACAGCT ACAAGGGCAA GGACTGCACT 
GTGCCCATCA ACACCTGCAT CCAGAACCCC TGTCAGCATG GAGGCACCTG CCACCTGAGT 
GACAGCCACA AGGATGGGTT CAGCTGCTCC TGCCCTCTGG GCTTTGAGGG GCAGCGGTGT 
GAGATCAACC CAGATGACTG TGAGGACAAC GACTGCGAAA ACAATGCCAC CTGCGTGGAC 
GGGATCAACA ACTACGTGTG TATCTGTCCG CCTAACTACA CAGGTGAGCT ATGCGACGAG 
GTGATTGACC ACTGTGTGCC TGAGCTGAAC CTCTGTCAGC ATGAGGCCAA GTGCATCCCC 
CTGGACAAAG GATTCAGCTG CGAGTGTGTC CCTGGCTACA GCGGGAAGCT CTGTGAGACA 
GACAATGATG ACTGTGTGGC CCACAAGTGC CGCCACGGGG CCCAGTGCGT GGACACAATC 
AATGGCTACA CATGCACCTG CCCCCAGGGC TTCAGTGGAC CCTTCTGTGA ACACCCCCCA 
CCCATGGTCC TACTGCAGAC CAGCCCATGC GACCAGTACG AGTGCCAGAA CGGGGCCCAG 
TGCATCGTGG TGCAGCAGGA GCCCACCTGC CGCTGCCCAC CAGGCTTCGC CGGCCCCAGA 
TGCGAGAAGC TCATCACTGT CAACTTCGTG GGCAAAGACT CCTACGTGGA ACTGGCCTCC 
GCCAAGGTCC GACCCCAGGC CAACATCTCC CTGCAGGTGG CCACTGACAA GGACAACGGC 
ATCCTTCTCT ACAAAGGAGA CAATGACCCC CTGGCACTGG AGCTGTACCA GGGCCACGTG 
CGGCTGGTCT ATGACAGCCT GAGTTCCCCT CCAACCACAG TGTACAGTGT GGAGACAGTG 
AATGATGGGC AGTTTCACAG TGTGGAGCTG GTGACGCTAA ACCAGACCCT GAACCTAGTA 
GTGGACAAAG GAACTCCAAA GAGCCTGGGG AAGCTCCAGA AGCAGCCAGC AGTGGGCATC 
AACAGCCCCC TCTACCTTGG AGGCATCCCC ACCTCCACCG GCCTCTCTGC CTTGCGCCAG 
GGCACGGACC GGCCTCTAGG CGGCTTCCAC GGATGCATCC ATGAGGTGCG CATCAACAAC 
GAGCTGCAGG ACTTCAAGGC CCTCCCACCA CAGTCCCTGG GGGTGTCACC AGGCTGCAAG 
TCCTGCACCG TGTGCAAGCA CGGCCTGTGC CGCTCCGTGG AGAAGGACAG CGTGGTGTGC 
GAGTGCCGCC CAGGCTGGAC CGGCCCACTC TGCGATCAGG AGGCCCGGGA CCCCTGCCTC 
GGCCACAGAT GCCACCATGG AAAATGTGTG GCAACTGGGA CCTCATACAT GTGCAAGTGT 
GCCGAGGGCT ATGGAGGGGA CTTGTGTGAC AACAAGAATG ACTCTGCCAA TGCCTGCTCA 
GCCTTCAAGT GTCACCATGG GCAGTGCCAC ATCTCAGACC AAGGGGAGCC CTACTGCCTG 
TGCCAGCCCG GCTTTAGCGG CGAGCACTGC CAACAAGAGA ATCCGTGCCT GGGACAAGTA 
GTCCGAGAGG TGATCCGCCG CCAGAAAGGT TATGCATCAT GTGCCACAGC CTCCAAGGTG 
CCCATCATGG AATGTCGTGG GGGCTGTGGG CCCCAGTGCT GCCAGCCCAC CCGCAGCAAG 
CGGCGGAAAT ACGTCTTCCA GTGCACGGAC GGCTCCTCGT TTGTAGAAGA GGTGGAGAGA 
CACTTAGAGT GCGGCTGCCT CGCGTGTTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT3 ( NM_001271946.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258875.1 Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 1, mRNA.

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CloneID	OHu12233
Clone ID Related Accession (Same CDS sequence)	NM_001271946.1
Accession Version	NM_001271946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	slit homolog 3 protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC146759.1, BC142665.1, AC011365.5 and HY285022.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC142665.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCCG GGTGGGCAGG GGTCGGCGCC GCCGTGCGCG CCCGCCTGGC GCTGGCCTTG 
GCGCTGGCGA GCGTCCTGAG TGGGCCTCCA GCCGTCGCCT GCCCCACCAA GTGTACCTGC 
TCCGCTGCCA GCGTGGACTG CCACGGGCTG GGCCTCCGCG CGGTTCCTCG GGGCATCCCC 
CGCAACGCTG AGCGCCTTGA CCTGGACAGA AATAATATCA CCAGGATCAC CAAGATGGAC 
TTCGCTGGGC TCAAGAACCT CCGAGTCTTG CATCTGGAAG ACAACCAGGT CAGCGTCATC 
GAGAGAGGCG CCTTCCAGGA CCTGAAGCAG CTAGAGCGAC TGCGCCTGAA CAAGAATAAG 
CTGCAAGTCC TTCCAGAATT GCTTTTCCAG AGCACGCCGA AGCTCACCAG ACTAGATTTG 
AGTGAAAACC AGATCCAGGG GATCCCGAGG AAGGCGTTCC GCGGCATCAC CGATGTGAAG 
AACCTGCAAC TGGACAACAA CCACATCAGC TGCATTGAAG ATGGAGCCTT CCGAGCGCTG 
CGCGATTTGG AGATCCTTAC CCTCAACAAC AACAACATCA GTCGCATCCT GGTCACCAGC 
TTCAACCACA TGCCGAAGAT CCGAACTCTG CGCCTCCACT CCAACCACCT GTACTGCGAC 
TGCCACCTGG CCTGGCTCTC GGATTGGCTG CGACAGCGAC GGACAGTTGG CCAGTTCACA 
CTCTGCATGG CTCCTGTGCA TTTGAGGGGC TTCAACGTGG CGGATGTGCA GAAGAAGGAG 
TACGTGTGCC CAGCCCCCCA CTCGGAGCCC CCATCCTGCA ATGCCAACTC CATCTCCTGC 
CCTTCGCCCT GCACGTGCAG CAATAACATC GTGGACTGTC GAGGAAAGGG CTTGATGGAG 
ATTCCTGCCA ACTTGCCGGA GGGCATCGTC GAAATACGCC TAGAACAGAA CTCCATCAAA 
GCCATCCCTG CAGGAGCCTT CACCCAGTAC AAGAAACTGA AGCGAATAGA CATCAGCAAG 
AATCAGATAT CGGATATTGC TCCAGATGCC TTCCAGGGCC TGAAATCACT CACATCGCTG 
GTCCTGTATG GGAACAAGAT CACCGAGATT GCCAAGGGAC TGTTTGATGG GCTGGTGTCC 
CTACAGCTGC TCCTCCTCAA TGCCAACAAG ATCAACTGCC TGCGGGTGAA CACGTTTCAG 
GACCTGCAGA ACCTCAACTT GCTCTCCCTG TATGACAACA AGCTGCAGAC CATCAGCAAG 
GGGCTCTTCG CCCCTCTGCA GTCCATCCAG ACACTCCACT TAGCCCAAAA CCCATTTGTG 
TGCGACTGCC ACTTGAAGTG GCTGGCCGAC TACCTCCAGG ACAACCCCAT CGAGACAAGC 
GGGGCCCGCT GCAGCAGCCC GCGCCGACTC GCCAACAAGC GCATCAGCCA GATCAAGAGC 
AAGAAGTTCC GCTGCTCAGG CTCCGAGGAT TACCGCAGCA GGTTCAGCAG CGAGTGCTTC 
ATGGACCTCG TGTGCCCCGA GAAGTGTCGC TGTGAGGGCA CGATTGTGGA CTGCTCCAAC 
CAGAAGCTGG TCCGCATCCC AAGCCACCTC CCTGAATATG TCACCGACCT GCGACTGAAT 
GACAATGAGG TATCTGTTCT GGAGGCCACT GGCATCTTCA AGAAGTTGCC CAACCTGCGG 
AAAATAAATC TGAGTAACAA TAAGATCAAG GAGGTGCGAG AGGGAGCTTT CGATGGAGCA 
GCCAGCGTGC AGGAGCTGAT GCTGACAGGG AACCAGCTGG AGACCGTGCA CGGGCGCGTG 
TTCCGTGGCC TCAGTGGCCT CAAAACCTTG ATGCTGAGGA GTAACTTGAT CGGCTGTGTG 
AGTAATGACA CCTTTGCCGG CCTGAGTTCG GTGAGACTGC TGTCCCTCTA TGACAATCGG 
ATCACCACCA TCACCCCTGG GGCCTTCACC ACGCTTGTCT CCCTGTCCAC CATAAACCTC 
CTGTCCAACC CCTTCAACTG CAACTGCCAC CTGGCCTGGC TCGGCAAGTG GTTGAGGAAG 
AGGCGGATCG TCAGTGGGAA CCCTAGGTGC CAGAAGCCAT TTTTCCTCAA GGAGATTCCC 
ATCCAGGATG TGGCCATCCA GGACTTCACC TGTGATGGCA ACGAGGAGAG TAGCTGCCAG 
CTGAGCCCGC GCTGCCCGGA GCAGTGCACC TGTATGGAGA CAGTGGTGCG ATGCAGCAAC 
AAGGGGCTCC GCGCCCTCCC CAGAGGCATG CCCAAGGATG TGACCGAGCT GTACCTGGAA 
GGAAACCACC TAACAGCCGT GCCCAGAGAG CTGTCCGCCC TCCGACACCT GACGCTTATT 
GACCTGAGCA ACAACAGCAT CAGCATGCTG ACCAATTACA CCTTCAGTAA CATGTCTCAC 
CTCTCCACTC TGATCCTGAG CTACAACCGG CTGAGGTGCA TCCCCGTCCA CGCCTTCAAC 
GGGCTGCGGT CCCTGCGAGT GCTAACCCTC CATGGCAATG ACATTTCCAG CGTTCCTGAA 
GGCTCCTTCA ACGACCTCAC ATCTCTTTCC CATCTGGCGC TGGGAACCAA CCCACTCCAC 
TGTGACTGCA GTCTTCGGTG GCTGTCGGAG TGGGTGAAGG CGGGGTACAA GGAGCCTGGC 
ATCGCCCGCT GCAGTAGCCC TGAGCCCATG GCTGACAGGC TCCTGCTCAC CACCCCAACC 
CACCGCTTCC AGTGCAAAGT ACTCTGGTTT TGCTGCCCAG GGCCAGTGGA CATCAACATT 
GTGGCCAAAT GCAATGCCTG CCTCTCCAGC CCGTGCAAGA ATAACGGGAC ATGCACCCAG 
GACCCTGTGG AGCTGTACCG CTGTGCCTGC CCCTACAGCT ACAAGGGCAA GGACTGCACT 
GTGCCCATCA ACACCTGCAT CCAGAACCCC TGTCAGCATG GAGGCACCTG CCACCTGAGT 
GACAGCCACA AGGATGGGTT CAGCTGCTCC TGCCCTCTGG GCTTTGAGGG GCAGCGGTGT 
GAGATCAACC CAGATGACTG TGAGGACAAC GACTGCGAAA ACAATGCCAC CTGCGTGGAC 
GGGATCAACA ACTACGTGTG TATCTGTCCG CCTAACTACA CAGGTGAGCT ATGCGACGAG 
GTGATTGACC ACTGTGTGCC TGAGCTGAAC CTCTGTCAGC ATGAGGCCAA GTGCATCCCC 
CTGGACAAAG GATTCAGCTG CGAGTGTGTC CCTGGCTACA GCGGGAAGCT CTGTGAGACA 
GACAATGATG ACTGTGTGGC CCACAAGTGC CGCCACGGGG CCCAGTGCGT GGACACAATC 
AATGGCTACA CATGCACCTG CCCCCAGGGC TTCAGTGGAC CCTTCTGTGA ACACCCCCCA 
CCCATGGTCC TACTGCAGAC CAGCCCATGC GACCAGTACG AGTGCCAGAA CGGGGCCCAG 
TGCATCGTGG TGCAGCAGGA GCCCACCTGC CGCTGCCCAC CAGGCTTCGC CGGCCCCAGA 
TGCGAGAAGC TCATCACTGT CAACTTCGTG GGCAAAGACT CCTACGTGGA ACTGGCCTCC 
GCCAAGGTCC GACCCCAGGC CAACATCTCC CTGCAGGTGG CCACTGACAA GGACAACGGC 
ATCCTTCTCT ACAAAGGAGA CAATGACCCC CTGGCACTGG AGCTGTACCA GGGCCACGTG 
CGGCTGGTCT ATGACAGCCT GAGTTCCCCT CCAACCACAG TGTACAGTGT GGAGACAGTG 
AATGATGGGC AGTTTCACAG TGTGGAGCTG GTGACGCTAA ACCAGACCCT GAACCTAGTA 
GTGGACAAAG GAACTCCAAA GAGCCTGGGG AAGCTCCAGA AGCAGCCAGC AGTGGGCATC 
AACAGCCCCC TCTACCTTGG AGGCATCCCC ACCTCCACCG GCCTCTCTGC CTTGCGCCAG 
GGCACGGACC GGCCTCTAGG CGGCTTCCAC GGATGCATCC ATGAGGTGCG CATCAACAAC 
GAGCTGCAGG ACTTCAAGGC CCTCCCACCA CAGTCCCTGG GGGTGTCACC AGGCTGCAAG 
TCCTGCACCG TGTGCAAGCA CGGCCTGTGC CGCTCCGTGG AGAAGGACAG CGTGGTGTGC 
GAGTGCCGCC CAGGCTGGAC CGGCCCACTC TGCGATCAGG AGGCCCGGGA CCCCTGCCTC 
GGCCACAGAT GCCACCATGG AAAATGTGTG GCAACTGGGA CCTCATACAT GTGCAAGTGT 
GCCGAGGGCT ATGGAGGGGA CTTGTGTGAC AACAAGAATG ACTCTGCCAA TGCCTGCTCA 
GCCTTCAAGT GTCACCATGG GCAGTGCCAC ATCTCAGACC AAGGGGAGCC CTACTGCCTG 
TGCCAGCCCG GCTTTAGCGG CGAGCACTGC CAACAAGAGA ATCCGTGCCT GGGACAAGTA 
GTCCGAGAGG TGATCCGCCG CCAGAAAGGT TATGCATCAT GTGCCACAGC CTCCAAGGTG 
CCCATCATGG AATGTCGTGG GGGCTGTGGG CCCCAGTGCT GCCAGCCCAC CCGCAGCAAG 
CGGCGGAAAT ACGTCTTCCA GTGCACGGAC GGCTCCTCGT TTGTAGAAGA GGTGGAGAGA 
CACTTAGAGT GCGGCTGCCT CGCGTGTTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258875.1
CDS	421..5013
Translation	MAPGWAGVGAAVRARLALALALASVLSGPPAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKVLWFCCPGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLDKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLLQTSPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQENPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQCCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGCLACS

Target ORF information:

RefSeq Version	NM_001271946.1
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271946.1

ORF Insert Sequence:

ATGGCCCCCG GGTGGGCAGG GGTCGGCGCC GCCGTGCGCG CCCGCCTGGC GCTGGCCTTG 
GCGCTGGCGA GCGTCCTGAG TGGGCCTCCA GCCGTCGCCT GCCCCACCAA GTGTACCTGC 
TCCGCTGCCA GCGTGGACTG CCACGGGCTG GGCCTCCGCG CGGTTCCTCG GGGCATCCCC 
CGCAACGCTG AGCGCCTTGA CCTGGACAGA AATAATATCA CCAGGATCAC CAAGATGGAC 
TTCGCTGGGC TCAAGAACCT CCGAGTCTTG CATCTGGAAG ACAACCAGGT CAGCGTCATC 
GAGAGAGGCG CCTTCCAGGA CCTGAAGCAG CTAGAGCGAC TGCGCCTGAA CAAGAATAAG 
CTGCAAGTCC TTCCAGAATT GCTTTTCCAG AGCACGCCGA AGCTCACCAG ACTAGATTTG 
AGTGAAAACC AGATCCAGGG GATCCCGAGG AAGGCGTTCC GCGGCATCAC CGATGTGAAG 
AACCTGCAAC TGGACAACAA CCACATCAGC TGCATTGAAG ATGGAGCCTT CCGAGCGCTG 
CGCGATTTGG AGATCCTTAC CCTCAACAAC AACAACATCA GTCGCATCCT GGTCACCAGC 
TTCAACCACA TGCCGAAGAT CCGAACTCTG CGCCTCCACT CCAACCACCT GTACTGCGAC 
TGCCACCTGG CCTGGCTCTC GGATTGGCTG CGACAGCGAC GGACAGTTGG CCAGTTCACA 
CTCTGCATGG CTCCTGTGCA TTTGAGGGGC TTCAACGTGG CGGATGTGCA GAAGAAGGAG 
TACGTGTGCC CAGCCCCCCA CTCGGAGCCC CCATCCTGCA ATGCCAACTC CATCTCCTGC 
CCTTCGCCCT GCACGTGCAG CAATAACATC GTGGACTGTC GAGGAAAGGG CTTGATGGAG 
ATTCCTGCCA ACTTGCCGGA GGGCATCGTC GAAATACGCC TAGAACAGAA CTCCATCAAA 
GCCATCCCTG CAGGAGCCTT CACCCAGTAC AAGAAACTGA AGCGAATAGA CATCAGCAAG 
AATCAGATAT CGGATATTGC TCCAGATGCC TTCCAGGGCC TGAAATCACT CACATCGCTG 
GTCCTGTATG GGAACAAGAT CACCGAGATT GCCAAGGGAC TGTTTGATGG GCTGGTGTCC 
CTACAGCTGC TCCTCCTCAA TGCCAACAAG ATCAACTGCC TGCGGGTGAA CACGTTTCAG 
GACCTGCAGA ACCTCAACTT GCTCTCCCTG TATGACAACA AGCTGCAGAC CATCAGCAAG 
GGGCTCTTCG CCCCTCTGCA GTCCATCCAG ACACTCCACT TAGCCCAAAA CCCATTTGTG 
TGCGACTGCC ACTTGAAGTG GCTGGCCGAC TACCTCCAGG ACAACCCCAT CGAGACAAGC 
GGGGCCCGCT GCAGCAGCCC GCGCCGACTC GCCAACAAGC GCATCAGCCA GATCAAGAGC 
AAGAAGTTCC GCTGCTCAGG CTCCGAGGAT TACCGCAGCA GGTTCAGCAG CGAGTGCTTC 
ATGGACCTCG TGTGCCCCGA GAAGTGTCGC TGTGAGGGCA CGATTGTGGA CTGCTCCAAC 
CAGAAGCTGG TCCGCATCCC AAGCCACCTC CCTGAATATG TCACCGACCT GCGACTGAAT 
GACAATGAGG TATCTGTTCT GGAGGCCACT GGCATCTTCA AGAAGTTGCC CAACCTGCGG 
AAAATAAATC TGAGTAACAA TAAGATCAAG GAGGTGCGAG AGGGAGCTTT CGATGGAGCA 
GCCAGCGTGC AGGAGCTGAT GCTGACAGGG AACCAGCTGG AGACCGTGCA CGGGCGCGTG 
TTCCGTGGCC TCAGTGGCCT CAAAACCTTG ATGCTGAGGA GTAACTTGAT CGGCTGTGTG 
AGTAATGACA CCTTTGCCGG CCTGAGTTCG GTGAGACTGC TGTCCCTCTA TGACAATCGG 
ATCACCACCA TCACCCCTGG GGCCTTCACC ACGCTTGTCT CCCTGTCCAC CATAAACCTC 
CTGTCCAACC CCTTCAACTG CAACTGCCAC CTGGCCTGGC TCGGCAAGTG GTTGAGGAAG 
AGGCGGATCG TCAGTGGGAA CCCTAGGTGC CAGAAGCCAT TTTTCCTCAA GGAGATTCCC 
ATCCAGGATG TGGCCATCCA GGACTTCACC TGTGATGGCA ACGAGGAGAG TAGCTGCCAG 
CTGAGCCCGC GCTGCCCGGA GCAGTGCACC TGTATGGAGA CAGTGGTGCG ATGCAGCAAC 
AAGGGGCTCC GCGCCCTCCC CAGAGGCATG CCCAAGGATG TGACCGAGCT GTACCTGGAA 
GGAAACCACC TAACAGCCGT GCCCAGAGAG CTGTCCGCCC TCCGACACCT GACGCTTATT 
GACCTGAGCA ACAACAGCAT CAGCATGCTG ACCAATTACA CCTTCAGTAA CATGTCTCAC 
CTCTCCACTC TGATCCTGAG CTACAACCGG CTGAGGTGCA TCCCCGTCCA CGCCTTCAAC 
GGGCTGCGGT CCCTGCGAGT GCTAACCCTC CATGGCAATG ACATTTCCAG CGTTCCTGAA 
GGCTCCTTCA ACGACCTCAC ATCTCTTTCC CATCTGGCGC TGGGAACCAA CCCACTCCAC 
TGTGACTGCA GTCTTCGGTG GCTGTCGGAG TGGGTGAAGG CGGGGTACAA GGAGCCTGGC 
ATCGCCCGCT GCAGTAGCCC TGAGCCCATG GCTGACAGGC TCCTGCTCAC CACCCCAACC 
CACCGCTTCC AGTGCAAAGT ACTCTGGTTT TGCTGCCCAG GGCCAGTGGA CATCAACATT 
GTGGCCAAAT GCAATGCCTG CCTCTCCAGC CCGTGCAAGA ATAACGGGAC ATGCACCCAG 
GACCCTGTGG AGCTGTACCG CTGTGCCTGC CCCTACAGCT ACAAGGGCAA GGACTGCACT 
GTGCCCATCA ACACCTGCAT CCAGAACCCC TGTCAGCATG GAGGCACCTG CCACCTGAGT 
GACAGCCACA AGGATGGGTT CAGCTGCTCC TGCCCTCTGG GCTTTGAGGG GCAGCGGTGT 
GAGATCAACC CAGATGACTG TGAGGACAAC GACTGCGAAA ACAATGCCAC CTGCGTGGAC 
GGGATCAACA ACTACGTGTG TATCTGTCCG CCTAACTACA CAGGTGAGCT ATGCGACGAG 
GTGATTGACC ACTGTGTGCC TGAGCTGAAC CTCTGTCAGC ATGAGGCCAA GTGCATCCCC 
CTGGACAAAG GATTCAGCTG CGAGTGTGTC CCTGGCTACA GCGGGAAGCT CTGTGAGACA 
GACAATGATG ACTGTGTGGC CCACAAGTGC CGCCACGGGG CCCAGTGCGT GGACACAATC 
AATGGCTACA CATGCACCTG CCCCCAGGGC TTCAGTGGAC CCTTCTGTGA ACACCCCCCA 
CCCATGGTCC TACTGCAGAC CAGCCCATGC GACCAGTACG AGTGCCAGAA CGGGGCCCAG 
TGCATCGTGG TGCAGCAGGA GCCCACCTGC CGCTGCCCAC CAGGCTTCGC CGGCCCCAGA 
TGCGAGAAGC TCATCACTGT CAACTTCGTG GGCAAAGACT CCTACGTGGA ACTGGCCTCC 
GCCAAGGTCC GACCCCAGGC CAACATCTCC CTGCAGGTGG CCACTGACAA GGACAACGGC 
ATCCTTCTCT ACAAAGGAGA CAATGACCCC CTGGCACTGG AGCTGTACCA GGGCCACGTG 
CGGCTGGTCT ATGACAGCCT GAGTTCCCCT CCAACCACAG TGTACAGTGT GGAGACAGTG 
AATGATGGGC AGTTTCACAG TGTGGAGCTG GTGACGCTAA ACCAGACCCT GAACCTAGTA 
GTGGACAAAG GAACTCCAAA GAGCCTGGGG AAGCTCCAGA AGCAGCCAGC AGTGGGCATC 
AACAGCCCCC TCTACCTTGG AGGCATCCCC ACCTCCACCG GCCTCTCTGC CTTGCGCCAG 
GGCACGGACC GGCCTCTAGG CGGCTTCCAC GGATGCATCC ATGAGGTGCG CATCAACAAC 
GAGCTGCAGG ACTTCAAGGC CCTCCCACCA CAGTCCCTGG GGGTGTCACC AGGCTGCAAG 
TCCTGCACCG TGTGCAAGCA CGGCCTGTGC CGCTCCGTGG AGAAGGACAG CGTGGTGTGC 
GAGTGCCGCC CAGGCTGGAC CGGCCCACTC TGCGATCAGG AGGCCCGGGA CCCCTGCCTC 
GGCCACAGAT GCCACCATGG AAAATGTGTG GCAACTGGGA CCTCATACAT GTGCAAGTGT 
GCCGAGGGCT ATGGAGGGGA CTTGTGTGAC AACAAGAATG ACTCTGCCAA TGCCTGCTCA 
GCCTTCAAGT GTCACCATGG GCAGTGCCAC ATCTCAGACC AAGGGGAGCC CTACTGCCTG 
TGCCAGCCCG GCTTTAGCGG CGAGCACTGC CAACAAGAGA ATCCGTGCCT GGGACAAGTA 
GTCCGAGAGG TGATCCGCCG CCAGAAAGGT TATGCATCAT GTGCCACAGC CTCCAAGGTG 
CCCATCATGG AATGTCGTGG GGGCTGTGGG CCCCAGTGCT GCCAGCCCAC CCGCAGCAAG 
CGGCGGAAAT ACGTCTTCCA GTGCACGGAC GGCTCCTCGT TTGTAGAAGA GGTGGAGAGA 
CACTTAGAGT GCGGCTGCCT CGCGTGTTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT3 ( NM_003062.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003053.1 Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 2, mRNA.

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CloneID	OHu20369
Clone ID Related Accession (Same CDS sequence)	NM_003062.3
Accession Version	NM_003062.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	slit homolog 3 protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC146759.1, AC011365.5 and HY285022.1. This sequence is a reference standard in the RefSeqGene project. On Dec 14, 2012 this sequence version replaced NM_003062.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146759.1, BC098388.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCCG GGTGGGCAGG GGTCGGCGCC GCCGTGCGCG CCCGCCTGGC GCTGGCCTTG 
GCGCTGGCGA GCGTCCTGAG TGGGCCTCCA GCCGTCGCCT GCCCCACCAA GTGTACCTGC 
TCCGCTGCCA GCGTGGACTG CCACGGGCTG GGCCTCCGCG CGGTTCCTCG GGGCATCCCC 
CGCAACGCTG AGCGCCTTGA CCTGGACAGA AATAATATCA CCAGGATCAC CAAGATGGAC 
TTCGCTGGGC TCAAGAACCT CCGAGTCTTG CATCTGGAAG ACAACCAGGT CAGCGTCATC 
GAGAGAGGCG CCTTCCAGGA CCTGAAGCAG CTAGAGCGAC TGCGCCTGAA CAAGAATAAG 
CTGCAAGTCC TTCCAGAATT GCTTTTCCAG AGCACGCCGA AGCTCACCAG ACTAGATTTG 
AGTGAAAACC AGATCCAGGG GATCCCGAGG AAGGCGTTCC GCGGCATCAC CGATGTGAAG 
AACCTGCAAC TGGACAACAA CCACATCAGC TGCATTGAAG ATGGAGCCTT CCGAGCGCTG 
CGCGATTTGG AGATCCTTAC CCTCAACAAC AACAACATCA GTCGCATCCT GGTCACCAGC 
TTCAACCACA TGCCGAAGAT CCGAACTCTG CGCCTCCACT CCAACCACCT GTACTGCGAC 
TGCCACCTGG CCTGGCTCTC GGATTGGCTG CGACAGCGAC GGACAGTTGG CCAGTTCACA 
CTCTGCATGG CTCCTGTGCA TTTGAGGGGC TTCAACGTGG CGGATGTGCA GAAGAAGGAG 
TACGTGTGCC CAGCCCCCCA CTCGGAGCCC CCATCCTGCA ATGCCAACTC CATCTCCTGC 
CCTTCGCCCT GCACGTGCAG CAATAACATC GTGGACTGTC GAGGAAAGGG CTTGATGGAG 
ATTCCTGCCA ACTTGCCGGA GGGCATCGTC GAAATACGCC TAGAACAGAA CTCCATCAAA 
GCCATCCCTG CAGGAGCCTT CACCCAGTAC AAGAAACTGA AGCGAATAGA CATCAGCAAG 
AATCAGATAT CGGATATTGC TCCAGATGCC TTCCAGGGCC TGAAATCACT CACATCGCTG 
GTCCTGTATG GGAACAAGAT CACCGAGATT GCCAAGGGAC TGTTTGATGG GCTGGTGTCC 
CTACAGCTGC TCCTCCTCAA TGCCAACAAG ATCAACTGCC TGCGGGTGAA CACGTTTCAG 
GACCTGCAGA ACCTCAACTT GCTCTCCCTG TATGACAACA AGCTGCAGAC CATCAGCAAG 
GGGCTCTTCG CCCCTCTGCA GTCCATCCAG ACACTCCACT TAGCCCAAAA CCCATTTGTG 
TGCGACTGCC ACTTGAAGTG GCTGGCCGAC TACCTCCAGG ACAACCCCAT CGAGACAAGC 
GGGGCCCGCT GCAGCAGCCC GCGCCGACTC GCCAACAAGC GCATCAGCCA GATCAAGAGC 
AAGAAGTTCC GCTGCTCAGG CTCCGAGGAT TACCGCAGCA GGTTCAGCAG CGAGTGCTTC 
ATGGACCTCG TGTGCCCCGA GAAGTGTCGC TGTGAGGGCA CGATTGTGGA CTGCTCCAAC 
CAGAAGCTGG TCCGCATCCC AAGCCACCTC CCTGAATATG TCACCGACCT GCGACTGAAT 
GACAATGAGG TATCTGTTCT GGAGGCCACT GGCATCTTCA AGAAGTTGCC CAACCTGCGG 
AAAATAAATC TGAGTAACAA TAAGATCAAG GAGGTGCGAG AGGGAGCTTT CGATGGAGCA 
GCCAGCGTGC AGGAGCTGAT GCTGACAGGG AACCAGCTGG AGACCGTGCA CGGGCGCGTG 
TTCCGTGGCC TCAGTGGCCT CAAAACCTTG ATGCTGAGGA GTAACTTGAT CGGCTGTGTG 
AGTAATGACA CCTTTGCCGG CCTGAGTTCG GTGAGACTGC TGTCCCTCTA TGACAATCGG 
ATCACCACCA TCACCCCTGG GGCCTTCACC ACGCTTGTCT CCCTGTCCAC CATAAACCTC 
CTGTCCAACC CCTTCAACTG CAACTGCCAC CTGGCCTGGC TCGGCAAGTG GTTGAGGAAG 
AGGCGGATCG TCAGTGGGAA CCCTAGGTGC CAGAAGCCAT TTTTCCTCAA GGAGATTCCC 
ATCCAGGATG TGGCCATCCA GGACTTCACC TGTGATGGCA ACGAGGAGAG TAGCTGCCAG 
CTGAGCCCGC GCTGCCCGGA GCAGTGCACC TGTATGGAGA CAGTGGTGCG ATGCAGCAAC 
AAGGGGCTCC GCGCCCTCCC CAGAGGCATG CCCAAGGATG TGACCGAGCT GTACCTGGAA 
GGAAACCACC TAACAGCCGT GCCCAGAGAG CTGTCCGCCC TCCGACACCT GACGCTTATT 
GACCTGAGCA ACAACAGCAT CAGCATGCTG ACCAATTACA CCTTCAGTAA CATGTCTCAC 
CTCTCCACTC TGATCCTGAG CTACAACCGG CTGAGGTGCA TCCCCGTCCA CGCCTTCAAC 
GGGCTGCGGT CCCTGCGAGT GCTAACCCTC CATGGCAATG ACATTTCCAG CGTTCCTGAA 
GGCTCCTTCA ACGACCTCAC ATCTCTTTCC CATCTGGCGC TGGGAACCAA CCCACTCCAC 
TGTGACTGCA GTCTTCGGTG GCTGTCGGAG TGGGTGAAGG CGGGGTACAA GGAGCCTGGC 
ATCGCCCGCT GCAGTAGCCC TGAGCCCATG GCTGACAGGC TCCTGCTCAC CACCCCAACC 
CACCGCTTCC AGTGCAAAGG GCCAGTGGAC ATCAACATTG TGGCCAAATG CAATGCCTGC 
CTCTCCAGCC CGTGCAAGAA TAACGGGACA TGCACCCAGG ACCCTGTGGA GCTGTACCGC 
TGTGCCTGCC CCTACAGCTA CAAGGGCAAG GACTGCACTG TGCCCATCAA CACCTGCATC 
CAGAACCCCT GTCAGCATGG AGGCACCTGC CACCTGAGTG ACAGCCACAA GGATGGGTTC 
AGCTGCTCCT GCCCTCTGGG CTTTGAGGGG CAGCGGTGTG AGATCAACCC AGATGACTGT 
GAGGACAACG ACTGCGAAAA CAATGCCACC TGCGTGGACG GGATCAACAA CTACGTGTGT 
ATCTGTCCGC CTAACTACAC AGGTGAGCTA TGCGACGAGG TGATTGACCA CTGTGTGCCT 
GAGCTGAACC TCTGTCAGCA TGAGGCCAAG TGCATCCCCC TGGACAAAGG ATTCAGCTGC 
GAGTGTGTCC CTGGCTACAG CGGGAAGCTC TGTGAGACAG ACAATGATGA CTGTGTGGCC 
CACAAGTGCC GCCACGGGGC CCAGTGCGTG GACACAATCA ATGGCTACAC ATGCACCTGC 
CCCCAGGGCT TCAGTGGACC CTTCTGTGAA CACCCCCCAC CCATGGTCCT ACTGCAGACC 
AGCCCATGCG ACCAGTACGA GTGCCAGAAC GGGGCCCAGT GCATCGTGGT GCAGCAGGAG 
CCCACCTGCC GCTGCCCACC AGGCTTCGCC GGCCCCAGAT GCGAGAAGCT CATCACTGTC 
AACTTCGTGG GCAAAGACTC CTACGTGGAA CTGGCCTCCG CCAAGGTCCG ACCCCAGGCC 
AACATCTCCC TGCAGGTGGC CACTGACAAG GACAACGGCA TCCTTCTCTA CAAAGGAGAC 
AATGACCCCC TGGCACTGGA GCTGTACCAG GGCCACGTGC GGCTGGTCTA TGACAGCCTG 
AGTTCCCCTC CAACCACAGT GTACAGTGTG GAGACAGTGA ATGATGGGCA GTTTCACAGT 
GTGGAGCTGG TGACGCTAAA CCAGACCCTG AACCTAGTAG TGGACAAAGG AACTCCAAAG 
AGCCTGGGGA AGCTCCAGAA GCAGCCAGCA GTGGGCATCA ACAGCCCCCT CTACCTTGGA 
GGCATCCCCA CCTCCACCGG CCTCTCTGCC TTGCGCCAGG GCACGGACCG GCCTCTAGGC 
GGCTTCCACG GATGCATCCA TGAGGTGCGC ATCAACAACG AGCTGCAGGA CTTCAAGGCC 
CTCCCACCAC AGTCCCTGGG GGTGTCACCA GGCTGCAAGT CCTGCACCGT GTGCAAGCAC 
GGCCTGTGCC GCTCCGTGGA GAAGGACAGC GTGGTGTGCG AGTGCCGCCC AGGCTGGACC 
GGCCCACTCT GCGATCAGGA GGCCCGGGAC CCCTGCCTCG GCCACAGATG CCACCATGGA 
AAATGTGTGG CAACTGGGAC CTCATACATG TGCAAGTGTG CCGAGGGCTA TGGAGGGGAC 
TTGTGTGACA ACAAGAATGA CTCTGCCAAT GCCTGCTCAG CCTTCAAGTG TCACCATGGG 
CAGTGCCACA TCTCAGACCA AGGGGAGCCC TACTGCCTGT GCCAGCCCGG CTTTAGCGGC 
GAGCACTGCC AACAAGAGAA TCCGTGCCTG GGACAAGTAG TCCGAGAGGT GATCCGCCGC 
CAGAAAGGTT ATGCATCATG TGCCACAGCC TCCAAGGTGC CCATCATGGA ATGTCGTGGG 
GGCTGTGGGC CCCAGTGCTG CCAGCCCACC CGCAGCAAGC GGCGGAAATA CGTCTTCCAG 
TGCACGGACG GCTCCTCGTT TGTAGAAGAG GTGGAGAGAC ACTTAGAGTG CGGCTGCCTC 
GCGTGTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003053.1
CDS	421..4992
Translation	MAPGWAGVGAAVRARLALALALASVLSGPPAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIAKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLDKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLLQTSPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQENPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQCCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGCLACS

Target ORF information:

RefSeq Version	NM_003062.3
Organism	Homo sapiens(human)
Definition	Homo sapiens slit guidance ligand 3 (SLIT3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003062.3

ORF Insert Sequence:

ATGGCCCCCG GGTGGGCAGG GGTCGGCGCC GCCGTGCGCG CCCGCCTGGC GCTGGCCTTG 
GCGCTGGCGA GCGTCCTGAG TGGGCCTCCA GCCGTCGCCT GCCCCACCAA GTGTACCTGC 
TCCGCTGCCA GCGTGGACTG CCACGGGCTG GGCCTCCGCG CGGTTCCTCG GGGCATCCCC 
CGCAACGCTG AGCGCCTTGA CCTGGACAGA AATAATATCA CCAGGATCAC CAAGATGGAC 
TTCGCTGGGC TCAAGAACCT CCGAGTCTTG CATCTGGAAG ACAACCAGGT CAGCGTCATC 
GAGAGAGGCG CCTTCCAGGA CCTGAAGCAG CTAGAGCGAC TGCGCCTGAA CAAGAATAAG 
CTGCAAGTCC TTCCAGAATT GCTTTTCCAG AGCACGCCGA AGCTCACCAG ACTAGATTTG 
AGTGAAAACC AGATCCAGGG GATCCCGAGG AAGGCGTTCC GCGGCATCAC CGATGTGAAG 
AACCTGCAAC TGGACAACAA CCACATCAGC TGCATTGAAG ATGGAGCCTT CCGAGCGCTG 
CGCGATTTGG AGATCCTTAC CCTCAACAAC AACAACATCA GTCGCATCCT GGTCACCAGC 
TTCAACCACA TGCCGAAGAT CCGAACTCTG CGCCTCCACT CCAACCACCT GTACTGCGAC 
TGCCACCTGG CCTGGCTCTC GGATTGGCTG CGACAGCGAC GGACAGTTGG CCAGTTCACA 
CTCTGCATGG CTCCTGTGCA TTTGAGGGGC TTCAACGTGG CGGATGTGCA GAAGAAGGAG 
TACGTGTGCC CAGCCCCCCA CTCGGAGCCC CCATCCTGCA ATGCCAACTC CATCTCCTGC 
CCTTCGCCCT GCACGTGCAG CAATAACATC GTGGACTGTC GAGGAAAGGG CTTGATGGAG 
ATTCCTGCCA ACTTGCCGGA GGGCATCGTC GAAATACGCC TAGAACAGAA CTCCATCAAA 
GCCATCCCTG CAGGAGCCTT CACCCAGTAC AAGAAACTGA AGCGAATAGA CATCAGCAAG 
AATCAGATAT CGGATATTGC TCCAGATGCC TTCCAGGGCC TGAAATCACT CACATCGCTG 
GTCCTGTATG GGAACAAGAT CACCGAGATT GCCAAGGGAC TGTTTGATGG GCTGGTGTCC 
CTACAGCTGC TCCTCCTCAA TGCCAACAAG ATCAACTGCC TGCGGGTGAA CACGTTTCAG 
GACCTGCAGA ACCTCAACTT GCTCTCCCTG TATGACAACA AGCTGCAGAC CATCAGCAAG 
GGGCTCTTCG CCCCTCTGCA GTCCATCCAG ACACTCCACT TAGCCCAAAA CCCATTTGTG 
TGCGACTGCC ACTTGAAGTG GCTGGCCGAC TACCTCCAGG ACAACCCCAT CGAGACAAGC 
GGGGCCCGCT GCAGCAGCCC GCGCCGACTC GCCAACAAGC GCATCAGCCA GATCAAGAGC 
AAGAAGTTCC GCTGCTCAGG CTCCGAGGAT TACCGCAGCA GGTTCAGCAG CGAGTGCTTC 
ATGGACCTCG TGTGCCCCGA GAAGTGTCGC TGTGAGGGCA CGATTGTGGA CTGCTCCAAC 
CAGAAGCTGG TCCGCATCCC AAGCCACCTC CCTGAATATG TCACCGACCT GCGACTGAAT 
GACAATGAGG TATCTGTTCT GGAGGCCACT GGCATCTTCA AGAAGTTGCC CAACCTGCGG 
AAAATAAATC TGAGTAACAA TAAGATCAAG GAGGTGCGAG AGGGAGCTTT CGATGGAGCA 
GCCAGCGTGC AGGAGCTGAT GCTGACAGGG AACCAGCTGG AGACCGTGCA CGGGCGCGTG 
TTCCGTGGCC TCAGTGGCCT CAAAACCTTG ATGCTGAGGA GTAACTTGAT CGGCTGTGTG 
AGTAATGACA CCTTTGCCGG CCTGAGTTCG GTGAGACTGC TGTCCCTCTA TGACAATCGG 
ATCACCACCA TCACCCCTGG GGCCTTCACC ACGCTTGTCT CCCTGTCCAC CATAAACCTC 
CTGTCCAACC CCTTCAACTG CAACTGCCAC CTGGCCTGGC TCGGCAAGTG GTTGAGGAAG 
AGGCGGATCG TCAGTGGGAA CCCTAGGTGC CAGAAGCCAT TTTTCCTCAA GGAGATTCCC 
ATCCAGGATG TGGCCATCCA GGACTTCACC TGTGATGGCA ACGAGGAGAG TAGCTGCCAG 
CTGAGCCCGC GCTGCCCGGA GCAGTGCACC TGTATGGAGA CAGTGGTGCG ATGCAGCAAC 
AAGGGGCTCC GCGCCCTCCC CAGAGGCATG CCCAAGGATG TGACCGAGCT GTACCTGGAA 
GGAAACCACC TAACAGCCGT GCCCAGAGAG CTGTCCGCCC TCCGACACCT GACGCTTATT 
GACCTGAGCA ACAACAGCAT CAGCATGCTG ACCAATTACA CCTTCAGTAA CATGTCTCAC 
CTCTCCACTC TGATCCTGAG CTACAACCGG CTGAGGTGCA TCCCCGTCCA CGCCTTCAAC 
GGGCTGCGGT CCCTGCGAGT GCTAACCCTC CATGGCAATG ACATTTCCAG CGTTCCTGAA 
GGCTCCTTCA ACGACCTCAC ATCTCTTTCC CATCTGGCGC TGGGAACCAA CCCACTCCAC 
TGTGACTGCA GTCTTCGGTG GCTGTCGGAG TGGGTGAAGG CGGGGTACAA GGAGCCTGGC 
ATCGCCCGCT GCAGTAGCCC TGAGCCCATG GCTGACAGGC TCCTGCTCAC CACCCCAACC 
CACCGCTTCC AGTGCAAAGG GCCAGTGGAC ATCAACATTG TGGCCAAATG CAATGCCTGC 
CTCTCCAGCC CGTGCAAGAA TAACGGGACA TGCACCCAGG ACCCTGTGGA GCTGTACCGC 
TGTGCCTGCC CCTACAGCTA CAAGGGCAAG GACTGCACTG TGCCCATCAA CACCTGCATC 
CAGAACCCCT GTCAGCATGG AGGCACCTGC CACCTGAGTG ACAGCCACAA GGATGGGTTC 
AGCTGCTCCT GCCCTCTGGG CTTTGAGGGG CAGCGGTGTG AGATCAACCC AGATGACTGT 
GAGGACAACG ACTGCGAAAA CAATGCCACC TGCGTGGACG GGATCAACAA CTACGTGTGT 
ATCTGTCCGC CTAACTACAC AGGTGAGCTA TGCGACGAGG TGATTGACCA CTGTGTGCCT 
GAGCTGAACC TCTGTCAGCA TGAGGCCAAG TGCATCCCCC TGGACAAAGG ATTCAGCTGC 
GAGTGTGTCC CTGGCTACAG CGGGAAGCTC TGTGAGACAG ACAATGATGA CTGTGTGGCC 
CACAAGTGCC GCCACGGGGC CCAGTGCGTG GACACAATCA ATGGCTACAC ATGCACCTGC 
CCCCAGGGCT TCAGTGGACC CTTCTGTGAA CACCCCCCAC CCATGGTCCT ACTGCAGACC 
AGCCCATGCG ACCAGTACGA GTGCCAGAAC GGGGCCCAGT GCATCGTGGT GCAGCAGGAG 
CCCACCTGCC GCTGCCCACC AGGCTTCGCC GGCCCCAGAT GCGAGAAGCT CATCACTGTC 
AACTTCGTGG GCAAAGACTC CTACGTGGAA CTGGCCTCCG CCAAGGTCCG ACCCCAGGCC 
AACATCTCCC TGCAGGTGGC CACTGACAAG GACAACGGCA TCCTTCTCTA CAAAGGAGAC 
AATGACCCCC TGGCACTGGA GCTGTACCAG GGCCACGTGC GGCTGGTCTA TGACAGCCTG 
AGTTCCCCTC CAACCACAGT GTACAGTGTG GAGACAGTGA ATGATGGGCA GTTTCACAGT 
GTGGAGCTGG TGACGCTAAA CCAGACCCTG AACCTAGTAG TGGACAAAGG AACTCCAAAG 
AGCCTGGGGA AGCTCCAGAA GCAGCCAGCA GTGGGCATCA ACAGCCCCCT CTACCTTGGA 
GGCATCCCCA CCTCCACCGG CCTCTCTGCC TTGCGCCAGG GCACGGACCG GCCTCTAGGC 
GGCTTCCACG GATGCATCCA TGAGGTGCGC ATCAACAACG AGCTGCAGGA CTTCAAGGCC 
CTCCCACCAC AGTCCCTGGG GGTGTCACCA GGCTGCAAGT CCTGCACCGT GTGCAAGCAC 
GGCCTGTGCC GCTCCGTGGA GAAGGACAGC GTGGTGTGCG AGTGCCGCCC AGGCTGGACC 
GGCCCACTCT GCGATCAGGA GGCCCGGGAC CCCTGCCTCG GCCACAGATG CCACCATGGA 
AAATGTGTGG CAACTGGGAC CTCATACATG TGCAAGTGTG CCGAGGGCTA TGGAGGGGAC 
TTGTGTGACA ACAAGAATGA CTCTGCCAAT GCCTGCTCAG CCTTCAAGTG TCACCATGGG 
CAGTGCCACA TCTCAGACCA AGGGGAGCCC TACTGCCTGT GCCAGCCCGG CTTTAGCGGC 
GAGCACTGCC AACAAGAGAA TCCGTGCCTG GGACAAGTAG TCCGAGAGGT GATCCGCCGC 
CAGAAAGGTT ATGCATCATG TGCCACAGCC TCCAAGGTGC CCATCATGGA ATGTCGTGGG 
GGCTGTGGGC CCCAGTGCTG CCAGCCCACC CGCAGCAAGC GGCGGAAATA CGTCTTCCAG 
TGCACGGACG GCTCCTCGTT TGTAGAAGAG GTGGAGAGAC ACTTAGAGTG CGGCTGCCTC 
GCGTGTTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Function of Slit/Robo signaling in breast cancer.
Frontiers of medicine9(4)431-6(2015 Dec)
Gu F,Ma Y,Zhang J,Qin F,Fu L

Effects of Slit3 silencing on the invasive ability of lung carcinoma A549 cells.
Oncology reports34(2)952-60(2015 Aug)
Zhang C,Guo H,Li B,Sui C,Zhang Y,Xia X,Qin Y,Ye L,Xie F,Wang H,Yuan M,Yuan L,Ye J

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SLIT3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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