slit3 cDNA ORF clone, Danio rerio(zebrafish)

The following slit3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slit3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa54914 XM_017359032.1
Latest version!
Danio rerio slit homolog 3 (Drosophila) (slit3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa39431 XM_009291043.3
Latest version!
Danio rerio slit homolog 3 (Drosophila) (slit3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa60483 NM_131736.3
Latest version!
Danio rerio slit homolog 3 (Drosophila) (slit3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
View Old Accession Versions >>
ODa39431 XM_009291043.2 Danio rerio slit homolog 3 (Drosophila) (slit3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa54914
    Clone ID Related Accession (Same CDS sequence) XM_017359032.1
    Accession Version XM_017359032.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product slit homolog 3 protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCCGCTT GTGTGCAACT GGACAGCAAC CACATCAGCT GCATCGAAGA TGGAGCCTTC 
    CGAGCCCTGC GCGATTTGGA GATACTCACT CTCAACAACA ACAATATCAC CCTCATCCCA
    CTGTCAAGCT TCAACCACAT GCCAAAGCTG CGGACACTTC GCCTGCACTC CAACAACCTG
    CACTGCGACT GCCACCTGTC CTGGCTCTCT GATTGGCTGC GGCAGAGGAG GGGTTTGGCC
    CCTTTCACAC AGTGCATGGC CCCCGCCCAT ATGAGAGGTC TCAATGTCCC TGATGTTCAA
    AAGAGAGAAT TTGTTTGCAC AGGTCCCGTA GAGACGGAGC CAAGGTCATG TGCTCCGCAG
    GTGACCATCT GCCCTGCCGC CTGCACCTGC AACAACAACA TTGTGGACTG CCGTAGAAAA
    GGCCTGACTG AGATTCCTGC CAACCTGCCT GAAGGAATCG TGGAAATCCG TCTGGAGCAG
    AACATGATCA AGAACATCCC AGCAGGAGCC TTCTCCACAT ACAAGAAACT CAAGAGAATA
    GACCTGAGCA AGAATCAGAT ATCCGAAATT GCAGAAGACG CATTTAGTGG CCTGCGCTCT
    CTCACGTCAC TGGTCCTGTA CGGAAATAAA ATCGCAGAGA TCCCAAAGGG ACTGTTTGAT
    GGCCTGGTCT CCCTGCAACT GCTACTGCTG AACGCCAACA AGATCAACTG CCTTCGAGTG
    AACACTTTCA AAGATCTGCA GAACCTCAAC CTGCTGTCCC TCTATGACAA CAAACTTCAG
    ACCATCAGCA AAGGCTTGTT CGCTCCTCTG CGTGCCATCA AAACCCTACA CCTCGCTCAG
    AATCCGTTCA TGTGTGACTG TCATCTGAAG TGGCTGGCCG ACTACCTGTT TGACAATCCA
    ATTGAGACCA GCGGGGCTCG ATGCAGCCAC CCGCGCCGCC TCGCCAACAA GCGCATCAGC
    CAGGTCAAGG GCAAGAAGTT TCGCTGCACA GGTGCGGAGG ACTACCGCAG TCGTCTCAGC
    GGCGAGTGTT TCCAAGACCT GGTTTGTCCC GAACGCTGCC GATGTGAAGG AACAGTAGTG
    GACTGCTCCA ACCTCAAACT CACCAAAATC CCCCCTCACC TTCCTGAGCA CACCACTGAC
    CTACGGCTGA ACGACAATGA AATCTCCATC TTAGAGGCTA CAGGAGCATT CAAGAAGCTT
    CCAAATCTGC GCAAGATAAA CTTGAGCAAT AATAAACTCC GGGACATCCG TGAGGGTGCG
    TTTGATGGGG CCGGAGGTGT TTTGGAGCTG CTGTTGACTG GAAACAAGCT GCAGTCGGTC
    AGCGGACGGA TGTTCAAGGG TTTGATCGGC CTCAAAACAC TGATGCTGAG GAGTAATCAG
    ATCAGCTGTG TGGATAACGC CACCTTCACC GGCTTGAGCT CTGTGCGCCT GCTTTCCCTC
    TACGATAACC GGATCTCCAC TATCGCCCCC GGAGCTTTCA ACACCCTGCA TTCCCTTTCC
    ACCATTAACC TGCTGTCTAA TCCCTACGTG TGTGACTGTC ACTTAGCCTG GCTGGGGCTG
    TGGCTTAAGA AGACCAGGGT GGTTAGCGGA AACCCACGCT GTCAGAAACC TGCCTTCCTG
    AAGGAGATCC CTATTCAAGA TGTGGCCATG CCTGATTTCT CCTGTGATGG TGCGGAAGAG
    AATGTGTGCC TGCCGTCAGC CCCTCGATGT CCAGAATCCT GCACATGCTC GGAAACCGTG
    GTGCGCTGTA GTAACCGTGG GCTGCGTTCT CTGCCTAAGA CTATCCCTAA AGATACAACT
    GAACTATACT TGGAAGGAAA CCTCCTCACA TCCGTTCCCA AAGAGCTTCC TAATCTGAAA
    CAACTGACAC TCCTAGACCT TAGTAATAAC AGCATCAGTC ATCTTGCCCC TCTCACCTTC
    AGCAACATGA CACAGCTCGC CACCCTTATC CTGAGCTATA ATCAAATACG CTGCGTCCCG
    GTTCATGCGT TTGATGGCTT GCGCTCTCTC CGCCTGCTAA CTCTCCACGG AAATGACCTT
    TCAACTGTTC CGGAGGGAGC CTTCAACCAC TTGACATCTC TATCTCATCT AGCTCTCGGT
    GCGAACCCTT TATACTGTGA CTGTGAGCTG CGCTGGTTGT CTCAGTGGGT GAAGGCTGGC
    TTTAAAGAGC CTGGCATCGC CCGCTGCACT GGACCACCCG ACATGGCTGA CCGACTGCTG
    CTTACCACCC CCCTCAATCG CTTCCAGTGC AAAGGTCCAG CAGATCTGAA TCTGATGTCC
    AAGTGCGCCC CCTGTCTGGC AACTCCCTGT CAGAATAATG GCACTTGTGT GAGTGATGTC
    ACAGGCTCCT ACCACTGCAC CTGCCCTTTT GGCTACAAGG GTCGTAACTG TGAGATTCCT
    ATTAACGCCT GTATCAGCCT GCCATGTACC AATGGAGGCA CGTGTCACCT CACACCTGGA
    CTGGATGGAC AGTACAGCTG CGTGTGTCCT CCTGGGTATG AGGGCCAGCA GTGTGAACTG
    AATCCCGATG ACTGTGAAGA CAACGATTGT GAGAATAACT CCACCTGTGT GGATGGCATC
    AACAACTACA CTTGTGTCTG CCCCCCTAAC TACAAAGGTG ATCTGTGCGA GGAGGTGGTT
    GACCCCTGTC TGCCTGGGTT TGACCTCTGC CAACACGACT CCAAGTGTGT TCCTCTCAGC
    AAGGGCTACA GATGTGAGTG TTTGCCTGGT TATGTTGGCC AGCAGTGTGA GCAGGATTAC
    AATGACTGTC TGGAGAACAA ATGTCAGCAT GGGGCAGAGT GTGTTGACGC CATCAATGGA
    TACACATGTG TGTGCAAGGA GGGCTTCAGT GGGCTATTCT GTGAGAATCA TCCACCAATG
    GTATTGCTCC AGAACACAAG TCCATGCGAT CAGTCGGATT GTCAAAATGG ATCCGAATGT
    CTGGTCATGG AAGGAGAGCC AGTGTGCCGT TGTCTGCCTG GTTACTTCGG CACTAAGTGT
    CAAAAAATGG TCACACTGCA CTTTCTCGGC AAGGAAGCTT ACGTGGAGCT CTCCGGCACC
    AGGATCCGAC CACCCATGCA CATTTCCTTC CAGGTAGCCA CTGATAAGGA TAATGGCATT
    CTGTTGTATA AAGAGGACCA TGACCCACTC GCCCTAGAAC TGTACCAGGG ACACATCCGT
    TTGATCTATG ACATTTCCAA ATACCCCCCA ACCACTGTGT ACAGTGTGGA GTCAGTGAAT
    GATGGGCTTT TCCACACGGT GGAGGTGCTG ATCCAGAATC AGACACTGAG TTTGGTGGTG
    GATAAAGGGA CTCCTAAAAG CTTGGGCAAA CTCCCCCGTC CCCCTGCTGT GGACCACAAC
    ACACAGCTAT ACATAGGCGG TGTGCCATCT CTGATTGGCA CATCAGGCCT TCGGCCGGGC
    CCATTCCAGT CCTTCAACGG CTGCCTTCAT GACGTACGCC TGAATGGAGA GCTCCAGGAC
    CTCGGTGCTG GACTGGCGGG GATAGAAGGC GTCCTGTTTG GGTGTCACTC CTGCAGCGTC
    TGTGACCAGG GAGCCTGCAG ACAAAGAGGA GACGAAGGGG TGACCTGTGA CTGCCCCACA
    GGACACAGCG GCCCACTATG TGAACAGATG AGCTCCCAAG ACCCCTGTGA AAGCAGCAGG
    TGTGTTCATG GGGAGTGTGT GGCGAAGGGC TCGTCGTACA GCTGTTTATG TGTGGATGGA
    TACTCAGGCC AGTTCTGTGA TAGACAGGAC GAGTCCAGCG CCTGCAAGGG ACTGCACTGT
    GTTTATGGAG AGTGCAGACT GACGGAGAGC GGAGAGCCCG CTTGTCACTG CCAGCCTGGA
    TACAGCGGGC CAACCTGCGA AACAGAACTG ACATGCGAAG GAGAAGCAGT TCGTGATTAT
    CTGTATCGCC AAATGGCTCA TAAAATCTGC ACGTCCACCA GCAGAGTCCA GCGGCTGGAG
    TGTCGCCACT CCTGCTCATC TGGGCTGTGC TGTGCTCCCA CCAAAAGCCG GAGACGGAAG
    CTCAGCTTCA AGTGTCCCGA TGGATCATCC TTTTCCGAGG AGATGGAGAT CACTGTGGAG
    TGTGGCTGCA CAAAGTGCTC ATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214521.1
    CDS16..4122
    Translation

    Target ORF information:

    RefSeq Version XM_017359032.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio slit homolog 3 (Drosophila) (slit3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017359032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCCGCTT GTGTGCAACT GGACAGCAAC CACATCAGCT GCATCGAAGA TGGAGCCTTC 
    CGAGCCCTGC GCGATTTGGA GATACTCACT CTCAACAACA ACAATATCAC CCTCATCCCA
    CTGTCAAGCT TCAACCACAT GCCAAAGCTG CGGACACTTC GCCTGCACTC CAACAACCTG
    CACTGCGACT GCCACCTGTC CTGGCTCTCT GATTGGCTGC GGCAGAGGAG GGGTTTGGCC
    CCTTTCACAC AGTGCATGGC CCCCGCCCAT ATGAGAGGTC TCAATGTCCC TGATGTTCAA
    AAGAGAGAAT TTGTTTGCAC AGGTCCCGTA GAGACGGAGC CAAGGTCATG TGCTCCGCAG
    GTGACCATCT GCCCTGCCGC CTGCACCTGC AACAACAACA TTGTGGACTG CCGTAGAAAA
    GGCCTGACTG AGATTCCTGC CAACCTGCCT GAAGGAATCG TGGAAATCCG TCTGGAGCAG
    AACATGATCA AGAACATCCC AGCAGGAGCC TTCTCCACAT ACAAGAAACT CAAGAGAATA
    GACCTGAGCA AGAATCAGAT ATCCGAAATT GCAGAAGACG CATTTAGTGG CCTGCGCTCT
    CTCACGTCAC TGGTCCTGTA CGGAAATAAA ATCGCAGAGA TCCCAAAGGG ACTGTTTGAT
    GGCCTGGTCT CCCTGCAACT GCTACTGCTG AACGCCAACA AGATCAACTG CCTTCGAGTG
    AACACTTTCA AAGATCTGCA GAACCTCAAC CTGCTGTCCC TCTATGACAA CAAACTTCAG
    ACCATCAGCA AAGGCTTGTT CGCTCCTCTG CGTGCCATCA AAACCCTACA CCTCGCTCAG
    AATCCGTTCA TGTGTGACTG TCATCTGAAG TGGCTGGCCG ACTACCTGTT TGACAATCCA
    ATTGAGACCA GCGGGGCTCG ATGCAGCCAC CCGCGCCGCC TCGCCAACAA GCGCATCAGC
    CAGGTCAAGG GCAAGAAGTT TCGCTGCACA GGTGCGGAGG ACTACCGCAG TCGTCTCAGC
    GGCGAGTGTT TCCAAGACCT GGTTTGTCCC GAACGCTGCC GATGTGAAGG AACAGTAGTG
    GACTGCTCCA ACCTCAAACT CACCAAAATC CCCCCTCACC TTCCTGAGCA CACCACTGAC
    CTACGGCTGA ACGACAATGA AATCTCCATC TTAGAGGCTA CAGGAGCATT CAAGAAGCTT
    CCAAATCTGC GCAAGATAAA CTTGAGCAAT AATAAACTCC GGGACATCCG TGAGGGTGCG
    TTTGATGGGG CCGGAGGTGT TTTGGAGCTG CTGTTGACTG GAAACAAGCT GCAGTCGGTC
    AGCGGACGGA TGTTCAAGGG TTTGATCGGC CTCAAAACAC TGATGCTGAG GAGTAATCAG
    ATCAGCTGTG TGGATAACGC CACCTTCACC GGCTTGAGCT CTGTGCGCCT GCTTTCCCTC
    TACGATAACC GGATCTCCAC TATCGCCCCC GGAGCTTTCA ACACCCTGCA TTCCCTTTCC
    ACCATTAACC TGCTGTCTAA TCCCTACGTG TGTGACTGTC ACTTAGCCTG GCTGGGGCTG
    TGGCTTAAGA AGACCAGGGT GGTTAGCGGA AACCCACGCT GTCAGAAACC TGCCTTCCTG
    AAGGAGATCC CTATTCAAGA TGTGGCCATG CCTGATTTCT CCTGTGATGG TGCGGAAGAG
    AATGTGTGCC TGCCGTCAGC CCCTCGATGT CCAGAATCCT GCACATGCTC GGAAACCGTG
    GTGCGCTGTA GTAACCGTGG GCTGCGTTCT CTGCCTAAGA CTATCCCTAA AGATACAACT
    GAACTATACT TGGAAGGAAA CCTCCTCACA TCCGTTCCCA AAGAGCTTCC TAATCTGAAA
    CAACTGACAC TCCTAGACCT TAGTAATAAC AGCATCAGTC ATCTTGCCCC TCTCACCTTC
    AGCAACATGA CACAGCTCGC CACCCTTATC CTGAGCTATA ATCAAATACG CTGCGTCCCG
    GTTCATGCGT TTGATGGCTT GCGCTCTCTC CGCCTGCTAA CTCTCCACGG AAATGACCTT
    TCAACTGTTC CGGAGGGAGC CTTCAACCAC TTGACATCTC TATCTCATCT AGCTCTCGGT
    GCGAACCCTT TATACTGTGA CTGTGAGCTG CGCTGGTTGT CTCAGTGGGT GAAGGCTGGC
    TTTAAAGAGC CTGGCATCGC CCGCTGCACT GGACCACCCG ACATGGCTGA CCGACTGCTG
    CTTACCACCC CCCTCAATCG CTTCCAGTGC AAAGGTCCAG CAGATCTGAA TCTGATGTCC
    AAGTGCGCCC CCTGTCTGGC AACTCCCTGT CAGAATAATG GCACTTGTGT GAGTGATGTC
    ACAGGCTCCT ACCACTGCAC CTGCCCTTTT GGCTACAAGG GTCGTAACTG TGAGATTCCT
    ATTAACGCCT GTATCAGCCT GCCATGTACC AATGGAGGCA CGTGTCACCT CACACCTGGA
    CTGGATGGAC AGTACAGCTG CGTGTGTCCT CCTGGGTATG AGGGCCAGCA GTGTGAACTG
    AATCCCGATG ACTGTGAAGA CAACGATTGT GAGAATAACT CCACCTGTGT GGATGGCATC
    AACAACTACA CTTGTGTCTG CCCCCCTAAC TACAAAGGTG ATCTGTGCGA GGAGGTGGTT
    GACCCCTGTC TGCCTGGGTT TGACCTCTGC CAACACGACT CCAAGTGTGT TCCTCTCAGC
    AAGGGCTACA GATGTGAGTG TTTGCCTGGT TATGTTGGCC AGCAGTGTGA GCAGGATTAC
    AATGACTGTC TGGAGAACAA ATGTCAGCAT GGGGCAGAGT GTGTTGACGC CATCAATGGA
    TACACATGTG TGTGCAAGGA GGGCTTCAGT GGGCTATTCT GTGAGAATCA TCCACCAATG
    GTATTGCTCC AGAACACAAG TCCATGCGAT CAGTCGGATT GTCAAAATGG ATCCGAATGT
    CTGGTCATGG AAGGAGAGCC AGTGTGCCGT TGTCTGCCTG GTTACTTCGG CACTAAGTGT
    CAAAAAATGG TCACACTGCA CTTTCTCGGC AAGGAAGCTT ACGTGGAGCT CTCCGGCACC
    AGGATCCGAC CACCCATGCA CATTTCCTTC CAGGTAGCCA CTGATAAGGA TAATGGCATT
    CTGTTGTATA AAGAGGACCA TGACCCACTC GCCCTAGAAC TGTACCAGGG ACACATCCGT
    TTGATCTATG ACATTTCCAA ATACCCCCCA ACCACTGTGT ACAGTGTGGA GTCAGTGAAT
    GATGGGCTTT TCCACACGGT GGAGGTGCTG ATCCAGAATC AGACACTGAG TTTGGTGGTG
    GATAAAGGGA CTCCTAAAAG CTTGGGCAAA CTCCCCCGTC CCCCTGCTGT GGACCACAAC
    ACACAGCTAT ACATAGGCGG TGTGCCATCT CTGATTGGCA CATCAGGCCT TCGGCCGGGC
    CCATTCCAGT CCTTCAACGG CTGCCTTCAT GACGTACGCC TGAATGGAGA GCTCCAGGAC
    CTCGGTGCTG GACTGGCGGG GATAGAAGGC GTCCTGTTTG GGTGTCACTC CTGCAGCGTC
    TGTGACCAGG GAGCCTGCAG ACAAAGAGGA GACGAAGGGG TGACCTGTGA CTGCCCCACA
    GGACACAGCG GCCCACTATG TGAACAGATG AGCTCCCAAG ACCCCTGTGA AAGCAGCAGG
    TGTGTTCATG GGGAGTGTGT GGCGAAGGGC TCGTCGTACA GCTGTTTATG TGTGGATGGA
    TACTCAGGCC AGTTCTGTGA TAGACAGGAC GAGTCCAGCG CCTGCAAGGG ACTGCACTGT
    GTTTATGGAG AGTGCAGACT GACGGAGAGC GGAGAGCCCG CTTGTCACTG CCAGCCTGGA
    TACAGCGGGC CAACCTGCGA AACAGAACTG ACATGCGAAG GAGAAGCAGT TCGTGATTAT
    CTGTATCGCC AAATGGCTCA TAAAATCTGC ACGTCCACCA GCAGAGTCCA GCGGCTGGAG
    TGTCGCCACT CCTGCTCATC TGGGCTGTGC TGTGCTCCCA CCAAAAGCCG GAGACGGAAG
    CTCAGCTTCA AGTGTCCCGA TGGATCATCC TTTTCCGAGG AGATGGAGAT CACTGTGGAG
    TGTGGCTGCA CAAAGTGCTC ATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39431
    Clone ID Related Accession (Same CDS sequence) XM_009291043.2 , XM_009291043.3
    Accession Version XM_009291043.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product slit homolog 3 protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCAAAGC TGCGGACACT TCGCCTGCAC TCCAACAACC TGCACTGCGA CTGCCACCTG 
    TCCTGGCTCT CTGATTGGCT GCGGCAGAGG AGGGGTTTGG CCCCTTTCAC ACAGTGCATG
    GCCCCCGCCC ATATGAGAGG TCTCAATGTC CCTGATGTTC AAAAGAGAGA ATTTGTTTGC
    ACAGGTCCCG TAGAGACGGA GCCAAGGTCA TGTGCTCCGC AGGTGACCAT CTGCCCTGCC
    GCCTGCACCT GCAACAACAA CATTGTGGAC TGCCGTAGAA AAGGCCTGAC TGAGATTCCT
    GCCAACCTGC CTGAAGGAAT CGTGGAAATC CGTCTGGAGC AGAACATGAT CAAGAACATC
    CCAGCAGGAG CCTTCTCCAC ATACAAGAAA CTCAAGAGAA TAGACCTGAG CAAGAATCAG
    ATATCCGAAA TTGCAGAAGA CGCATTTAGT GGCCTGCGCT CTCTCACGTC ACTGGTCCTG
    TACGGAAATA AAATCGCAGA GATCCCAAAG GGACTGTTTG ATGGCCTGGT CTCCCTGCAA
    CTGCTACTGC TGAACGCCAA CAAGATCAAC TGCCTTCGAG TGAACACTTT CAAAGATCTG
    CAGAACCTCA ACCTGCTGTC CCTCTATGAC AACAAACTTC AGACCATCAG CAAAGGCTTG
    TTCGCTCCTC TGCGTGCCAT CAAAACCCTA CACCTCGCTC AGAATCCGTT CATGTGTGAC
    TGTCATCTGA AGTGGCTGGC CGACTACCTG TTTGACAATC CAATTGAGAC CAGCGGGGCT
    CGATGCAGCC ACCCGCGCCG CCTCGCCAAC AAGCGCATCA GCCAGGTCAA GGGCAAGAAG
    TTTCGCTGCA CAGGTGCGGA GGACTACCGC AGTCGTCTCA GCGGCGAGTG TTTCCAAGAC
    CTGGTTTGTC CCGAACGCTG CCGATGTGAA GGAACAGTAG TGGACTGCTC CAACCTCAAA
    CTCACCAAAA TCCCCCCTCA CCTTCCTGAG CACACCACTG ACCTACGGCT GAACGACAAT
    GAAATCTCCA TCTTAGAGGC TACAGGAGCA TTCAAGAAGC TTCCAAATCT GCGCAAGATA
    AACTTGAGCA ATAATAAACT CCGGGACATC CGTGAGGGTG CGTTTGATGG GGCCGGAGGT
    GTTTTGGAGC TGCTGTTGAC TGGAAACAAG CTGCAGTCGG TCAGCGGACG GATGTTCAAG
    GGTTTGATCG GCCTCAAAAC ACTGATGCTG AGGAGTAATC AGATCAGCTG TGTGGATAAC
    GCCACCTTCA CCGGCTTGAG CTCTGTGCGC CTGCTTTCCC TCTACGATAA CCGGATCTCC
    ACTATCGCCC CCGGAGCTTT CAACACCCTG CATTCCCTTT CCACCATTAA CCTGCTGTCT
    AATCCCTACG TGTGTGACTG TCACTTAGCC TGGCTGGGGC TGTGGCTTAA GAAGACCAGG
    GTGGTTAGCG GAAACCCACG CTGTCAGAAA CCTGCCTTCC TGAAGGAGAT CCCTATTCAA
    GATGTGGCCA TGCCTGATTT CTCCTGTGAT GGTGCGGAAG AGAATGTGTG CCTGCCGTCA
    GCCCCTCGAT GTCCAGAATC CTGCACATGC TCGGAAACCG TGGTGCGCTG TAGTAACCGT
    GGGCTGCGTT CTCTGCCTAA GACTATCCCT AAAGATACAA CTGAACTATA CTTGGAAGGA
    AACCTCCTCA CATCCGTTCC CAAAGAGCTT CCTAATCTGA AACAACTGAC ACTCCTAGAC
    CTTAGTAATA ACAGCATCAG TCATCTTGCC CCTCTCACCT TCAGCAACAT GACACAGCTC
    GCCACCCTTA TCCTGAGCTA TAATCAAATA CGCTGCGTCC CGGTTCATGC GTTTGATGGC
    TTGCGCTCTC TCCGCCTGCT AACTCTCCAC GGAAATGACC TTTCAACTGT TCCGGAGGGA
    GCCTTCAACC ACTTGACATC TCTATCTCAT CTAGCTCTCG GTGCGAACCC TTTATACTGT
    GACTGTGAGC TGCGCTGGTT GTCTCAGTGG GTGAAGGCTG GCTTTAAAGA GCCTGGCATC
    GCCCGCTGCA CTGGACCACC CGACATGGCT GACCGACTGC TGCTTACCAC CCCCCTCAAT
    CGCTTCCAGT GCAAAGGTCC AGCAGATCTG AATCTGATGT CCAAGTGCGC CCCCTGTCTG
    GCAACTCCCT GTCAGAATAA TGGCACTTGT GTGAGTGATG TCACAGGCTC CTACCACTGC
    ACCTGCCCTT TTGGCTACAA GGGTCGTAAC TGTGAGATTC CTATTAACGC CTGTATCAGC
    CTGCCATGTA CCAATGGAGG CACGTGTCAC CTCACACCTG GACTGGATGG ACAGTACAGC
    TGCGTGTGTC CTCCTGGGTA TGAGGGCCAG CAGTGTGAAC TGAATCCCGA TGACTGTGAA
    GACAACGATT GTGAGAATAA CTCCACCTGT GTGGATGGCA TCAACAACTA CACTTGTGTC
    TGCCCCCCTA ACTACAAAGG TGATCTGTGC GAGGAGGTGG TTGACCCCTG TCTGCCTGGG
    TTTGACCTCT GCCAACACGA CTCCAAGTGT GTTCCTCTCA GCAAGGGCTA CAGATGTGAG
    TGTTTGCCTG GTTATGTTGG CCAGCAGTGT GAGCAGGATT ACAATGACTG TCTGGAGAAC
    AAATGTCAGC ATGGGGCAGA GTGTGTTGAC GCCATCAATG GATACACATG TGTGTGCAAG
    GAGGGCTTCA GTGGGCTATT CTGTGAGAAT CATCCACCAA TGGTATTGCT CCAGAACACA
    AGTCCATGCG ATCAGTCGGA TTGTCAAAAT GGATCCGAAT GTCTGGTCAT GGAAGGAGAG
    CCAGTGTGCC GTTGTCTGCC TGGTTACTTC GGCACTAAGT GTCAAAAAAT GGTCACACTG
    CACTTTCTCG GCAAGGAAGC TTACGTGGAG CTCTCCGGCA CCAGGATCCG ACCACCCATG
    CACATTTCCT TCCAGGTAGC CACTGATAAG GATAATGGCA TTCTGTTGTA TAAAGAGGAC
    CATGACCCAC TCGCCCTAGA ACTGTACCAG GGACACATCC GTTTGATCTA TGACATTTCC
    AAATACCCCC CAACCACTGT GTACAGTGTG GAGTCAGTGA ATGATGGGCT TTTCCACACG
    GTGGAGGTGC TGATCCAGAA TCAGACACTG AGTTTGGTGG TGGATAAAGG GACTCCTAAA
    AGCTTGGGCA AACTCCCCCG TCCCCCTGCT GTGGACCACA ACACACAGCT ATACATAGGC
    GGTGTGCCAT CTCTGATTGG CACATCAGGC CTTCGGCCGG GCCCATTCCA GTCCTTCAAC
    GGCTGCCTTC ATGACGTACG CCTGAATGGA GAGCTCCAGG ACCTCGGTGC TGGACTGGCG
    GGGATAGAAG GCGTCCTGTT TGGGTGTCAC TCCTGCAGCG TCTGTGACCA GGGAGCCTGC
    AGACAAAGAG GAGACGAAGG GGTGACCTGT GACTGCCCCA CAGGACACAG CGGCCCACTA
    TGTGAACAGA TGAGCTCCCA AGACCCCTGT GAAAGCAGCA GGTGTGTTCA TGGGGAGTGT
    GTGGCGAAGG GCTCGTCGTA CAGCTGTTTA TGTGTGGATG GATACTCAGG CCAGTTCTGT
    GATAGACAGG ACGAGTCCAG CGCCTGCAAG GGACTGCACT GTGTTTATGG AGAGTGCAGA
    CTGACGGAGA GCGGAGAGCC CGCTTGTCAC TGCCAGCCTG GATACAGCGG GCCAACCTGC
    GAAACAGAAC TGACATGCGA AGGAGAAGCA GTTCGTGATT ATCTGTATCG CCAAATGGCT
    CATAAAATCT GCACGTCCAC CAGCAGAGTC CAGCGGCTGG AGTGTCGCCA CTCCTGCTCA
    TCTGGGCTGT GCTGTGCTCC CACCAAAAGC CGGAGACGGA AGCTCAGCTT CAAGTGTCCC
    GATGGATCAT CCTTTTCCGA GGAGATGGAG ATCACTGTGG AGTGTGGCTG CACAAAGTGC
    TCATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289318.1
    CDS303..4271
    Translation

    Target ORF information:

    RefSeq Version XM_009291043.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio slit homolog 3 (Drosophila) (slit3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291043.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCAAAGC TGCGGACACT TCGCCTGCAC TCCAACAACC TGCACTGCGA CTGCCACCTG 
    TCCTGGCTCT CTGATTGGCT GCGGCAGAGG AGGGGTTTGG CCCCTTTCAC ACAGTGCATG
    GCCCCCGCCC ATATGAGAGG TCTCAATGTC CCTGATGTTC AAAAGAGAGA ATTTGTTTGC
    ACAGGTCCCG TAGAGACGGA GCCAAGGTCA TGTGCTCCGC AGGTGACCAT CTGCCCTGCC
    GCCTGCACCT GCAACAACAA CATTGTGGAC TGCCGTAGAA AAGGCCTGAC TGAGATTCCT
    GCCAACCTGC CTGAAGGAAT CGTGGAAATC CGTCTGGAGC AGAACATGAT CAAGAACATC
    CCAGCAGGAG CCTTCTCCAC ATACAAGAAA CTCAAGAGAA TAGACCTGAG CAAGAATCAG
    ATATCCGAAA TTGCAGAAGA CGCATTTAGT GGCCTGCGCT CTCTCACGTC ACTGGTCCTG
    TACGGAAATA AAATCGCAGA GATCCCAAAG GGACTGTTTG ATGGCCTGGT CTCCCTGCAA
    CTGCTACTGC TGAACGCCAA CAAGATCAAC TGCCTTCGAG TGAACACTTT CAAAGATCTG
    CAGAACCTCA ACCTGCTGTC CCTCTATGAC AACAAACTTC AGACCATCAG CAAAGGCTTG
    TTCGCTCCTC TGCGTGCCAT CAAAACCCTA CACCTCGCTC AGAATCCGTT CATGTGTGAC
    TGTCATCTGA AGTGGCTGGC CGACTACCTG TTTGACAATC CAATTGAGAC CAGCGGGGCT
    CGATGCAGCC ACCCGCGCCG CCTCGCCAAC AAGCGCATCA GCCAGGTCAA GGGCAAGAAG
    TTTCGCTGCA CAGGTGCGGA GGACTACCGC AGTCGTCTCA GCGGCGAGTG TTTCCAAGAC
    CTGGTTTGTC CCGAACGCTG CCGATGTGAA GGAACAGTAG TGGACTGCTC CAACCTCAAA
    CTCACCAAAA TCCCCCCTCA CCTTCCTGAG CACACCACTG ACCTACGGCT GAACGACAAT
    GAAATCTCCA TCTTAGAGGC TACAGGAGCA TTCAAGAAGC TTCCAAATCT GCGCAAGATA
    AACTTGAGCA ATAATAAACT CCGGGACATC CGTGAGGGTG CGTTTGATGG GGCCGGAGGT
    GTTTTGGAGC TGCTGTTGAC TGGAAACAAG CTGCAGTCGG TCAGCGGACG GATGTTCAAG
    GGTTTGATCG GCCTCAAAAC ACTGATGCTG AGGAGTAATC AGATCAGCTG TGTGGATAAC
    GCCACCTTCA CCGGCTTGAG CTCTGTGCGC CTGCTTTCCC TCTACGATAA CCGGATCTCC
    ACTATCGCCC CCGGAGCTTT CAACACCCTG CATTCCCTTT CCACCATTAA CCTGCTGTCT
    AATCCCTACG TGTGTGACTG TCACTTAGCC TGGCTGGGGC TGTGGCTTAA GAAGACCAGG
    GTGGTTAGCG GAAACCCACG CTGTCAGAAA CCTGCCTTCC TGAAGGAGAT CCCTATTCAA
    GATGTGGCCA TGCCTGATTT CTCCTGTGAT GGTGCGGAAG AGAATGTGTG CCTGCCGTCA
    GCCCCTCGAT GTCCAGAATC CTGCACATGC TCGGAAACCG TGGTGCGCTG TAGTAACCGT
    GGGCTGCGTT CTCTGCCTAA GACTATCCCT AAAGATACAA CTGAACTATA CTTGGAAGGA
    AACCTCCTCA CATCCGTTCC CAAAGAGCTT CCTAATCTGA AACAACTGAC ACTCCTAGAC
    CTTAGTAATA ACAGCATCAG TCATCTTGCC CCTCTCACCT TCAGCAACAT GACACAGCTC
    GCCACCCTTA TCCTGAGCTA TAATCAAATA CGCTGCGTCC CGGTTCATGC GTTTGATGGC
    TTGCGCTCTC TCCGCCTGCT AACTCTCCAC GGAAATGACC TTTCAACTGT TCCGGAGGGA
    GCCTTCAACC ACTTGACATC TCTATCTCAT CTAGCTCTCG GTGCGAACCC TTTATACTGT
    GACTGTGAGC TGCGCTGGTT GTCTCAGTGG GTGAAGGCTG GCTTTAAAGA GCCTGGCATC
    GCCCGCTGCA CTGGACCACC CGACATGGCT GACCGACTGC TGCTTACCAC CCCCCTCAAT
    CGCTTCCAGT GCAAAGGTCC AGCAGATCTG AATCTGATGT CCAAGTGCGC CCCCTGTCTG
    GCAACTCCCT GTCAGAATAA TGGCACTTGT GTGAGTGATG TCACAGGCTC CTACCACTGC
    ACCTGCCCTT TTGGCTACAA GGGTCGTAAC TGTGAGATTC CTATTAACGC CTGTATCAGC
    CTGCCATGTA CCAATGGAGG CACGTGTCAC CTCACACCTG GACTGGATGG ACAGTACAGC
    TGCGTGTGTC CTCCTGGGTA TGAGGGCCAG CAGTGTGAAC TGAATCCCGA TGACTGTGAA
    GACAACGATT GTGAGAATAA CTCCACCTGT GTGGATGGCA TCAACAACTA CACTTGTGTC
    TGCCCCCCTA ACTACAAAGG TGATCTGTGC GAGGAGGTGG TTGACCCCTG TCTGCCTGGG
    TTTGACCTCT GCCAACACGA CTCCAAGTGT GTTCCTCTCA GCAAGGGCTA CAGATGTGAG
    TGTTTGCCTG GTTATGTTGG CCAGCAGTGT GAGCAGGATT ACAATGACTG TCTGGAGAAC
    AAATGTCAGC ATGGGGCAGA GTGTGTTGAC GCCATCAATG GATACACATG TGTGTGCAAG
    GAGGGCTTCA GTGGGCTATT CTGTGAGAAT CATCCACCAA TGGTATTGCT CCAGAACACA
    AGTCCATGCG ATCAGTCGGA TTGTCAAAAT GGATCCGAAT GTCTGGTCAT GGAAGGAGAG
    CCAGTGTGCC GTTGTCTGCC TGGTTACTTC GGCACTAAGT GTCAAAAAAT GGTCACACTG
    CACTTTCTCG GCAAGGAAGC TTACGTGGAG CTCTCCGGCA CCAGGATCCG ACCACCCATG
    CACATTTCCT TCCAGGTAGC CACTGATAAG GATAATGGCA TTCTGTTGTA TAAAGAGGAC
    CATGACCCAC TCGCCCTAGA ACTGTACCAG GGACACATCC GTTTGATCTA TGACATTTCC
    AAATACCCCC CAACCACTGT GTACAGTGTG GAGTCAGTGA ATGATGGGCT TTTCCACACG
    GTGGAGGTGC TGATCCAGAA TCAGACACTG AGTTTGGTGG TGGATAAAGG GACTCCTAAA
    AGCTTGGGCA AACTCCCCCG TCCCCCTGCT GTGGACCACA ACACACAGCT ATACATAGGC
    GGTGTGCCAT CTCTGATTGG CACATCAGGC CTTCGGCCGG GCCCATTCCA GTCCTTCAAC
    GGCTGCCTTC ATGACGTACG CCTGAATGGA GAGCTCCAGG ACCTCGGTGC TGGACTGGCG
    GGGATAGAAG GCGTCCTGTT TGGGTGTCAC TCCTGCAGCG TCTGTGACCA GGGAGCCTGC
    AGACAAAGAG GAGACGAAGG GGTGACCTGT GACTGCCCCA CAGGACACAG CGGCCCACTA
    TGTGAACAGA TGAGCTCCCA AGACCCCTGT GAAAGCAGCA GGTGTGTTCA TGGGGAGTGT
    GTGGCGAAGG GCTCGTCGTA CAGCTGTTTA TGTGTGGATG GATACTCAGG CCAGTTCTGT
    GATAGACAGG ACGAGTCCAG CGCCTGCAAG GGACTGCACT GTGTTTATGG AGAGTGCAGA
    CTGACGGAGA GCGGAGAGCC CGCTTGTCAC TGCCAGCCTG GATACAGCGG GCCAACCTGC
    GAAACAGAAC TGACATGCGA AGGAGAAGCA GTTCGTGATT ATCTGTATCG CCAAATGGCT
    CATAAAATCT GCACGTCCAC CAGCAGAGTC CAGCGGCTGG AGTGTCGCCA CTCCTGCTCA
    TCTGGGCTGT GCTGTGCTCC CACCAAAAGC CGGAGACGGA AGCTCAGCTT CAAGTGTCCC
    GATGGATCAT CCTTTTCCGA GGAGATGGAG ATCACTGTGG AGTGTGGCTG CACAAAGTGC
    TCATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39431
    Clone ID Related Accession (Same CDS sequence) XM_009291043.2 , XM_009291043.3
    Accession Version XM_009291043.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 3 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCAAAGC TGCGGACACT TCGCCTGCAC TCCAACAACC TGCACTGCGA CTGCCACCTG 
    TCCTGGCTCT CTGATTGGCT GCGGCAGAGG AGGGGTTTGG CCCCTTTCAC ACAGTGCATG
    GCCCCCGCCC ATATGAGAGG TCTCAATGTC CCTGATGTTC AAAAGAGAGA ATTTGTTTGC
    ACAGGTCCCG TAGAGACGGA GCCAAGGTCA TGTGCTCCGC AGGTGACCAT CTGCCCTGCC
    GCCTGCACCT GCAACAACAA CATTGTGGAC TGCCGTAGAA AAGGCCTGAC TGAGATTCCT
    GCCAACCTGC CTGAAGGAAT CGTGGAAATC CGTCTGGAGC AGAACATGAT CAAGAACATC
    CCAGCAGGAG CCTTCTCCAC ATACAAGAAA CTCAAGAGAA TAGACCTGAG CAAGAATCAG
    ATATCCGAAA TTGCAGAAGA CGCATTTAGT GGCCTGCGCT CTCTCACGTC ACTGGTCCTG
    TACGGAAATA AAATCGCAGA GATCCCAAAG GGACTGTTTG ATGGCCTGGT CTCCCTGCAA
    CTGCTACTGC TGAACGCCAA CAAGATCAAC TGCCTTCGAG TGAACACTTT CAAAGATCTG
    CAGAACCTCA ACCTGCTGTC CCTCTATGAC AACAAACTTC AGACCATCAG CAAAGGCTTG
    TTCGCTCCTC TGCGTGCCAT CAAAACCCTA CACCTCGCTC AGAATCCGTT CATGTGTGAC
    TGTCATCTGA AGTGGCTGGC CGACTACCTG TTTGACAATC CAATTGAGAC CAGCGGGGCT
    CGATGCAGCC ACCCGCGCCG CCTCGCCAAC AAGCGCATCA GCCAGGTCAA GGGCAAGAAG
    TTTCGCTGCA CAGGTGCGGA GGACTACCGC AGTCGTCTCA GCGGCGAGTG TTTCCAAGAC
    CTGGTTTGTC CCGAACGCTG CCGATGTGAA GGAACAGTAG TGGACTGCTC CAACCTCAAA
    CTCACCAAAA TCCCCCCTCA CCTTCCTGAG CACACCACTG ACCTACGGCT GAACGACAAT
    GAAATCTCCA TCTTAGAGGC TACAGGAGCA TTCAAGAAGC TTCCAAATCT GCGCAAGATA
    AACTTGAGCA ATAATAAACT CCGGGACATC CGTGAGGGTG CGTTTGATGG GGCCGGAGGT
    GTTTTGGAGC TGCTGTTGAC TGGAAACAAG CTGCAGTCGG TCAGCGGACG GATGTTCAAG
    GGTTTGATCG GCCTCAAAAC ACTGATGCTG AGGAGTAATC AGATCAGCTG TGTGGATAAC
    GCCACCTTCA CCGGCTTGAG CTCTGTGCGC CTGCTTTCCC TCTACGATAA CCGGATCTCC
    ACTATCGCCC CCGGAGCTTT CAACACCCTG CATTCCCTTT CCACCATTAA CCTGCTGTCT
    AATCCCTACG TGTGTGACTG TCACTTAGCC TGGCTGGGGC TGTGGCTTAA GAAGACCAGG
    GTGGTTAGCG GAAACCCACG CTGTCAGAAA CCTGCCTTCC TGAAGGAGAT CCCTATTCAA
    GATGTGGCCA TGCCTGATTT CTCCTGTGAT GGTGCGGAAG AGAATGTGTG CCTGCCGTCA
    GCCCCTCGAT GTCCAGAATC CTGCACATGC TCGGAAACCG TGGTGCGCTG TAGTAACCGT
    GGGCTGCGTT CTCTGCCTAA GACTATCCCT AAAGATACAA CTGAACTATA CTTGGAAGGA
    AACCTCCTCA CATCCGTTCC CAAAGAGCTT CCTAATCTGA AACAACTGAC ACTCCTAGAC
    CTTAGTAATA ACAGCATCAG TCATCTTGCC CCTCTCACCT TCAGCAACAT GACACAGCTC
    GCCACCCTTA TCCTGAGCTA TAATCAAATA CGCTGCGTCC CGGTTCATGC GTTTGATGGC
    TTGCGCTCTC TCCGCCTGCT AACTCTCCAC GGAAATGACC TTTCAACTGT TCCGGAGGGA
    GCCTTCAACC ACTTGACATC TCTATCTCAT CTAGCTCTCG GTGCGAACCC TTTATACTGT
    GACTGTGAGC TGCGCTGGTT GTCTCAGTGG GTGAAGGCTG GCTTTAAAGA GCCTGGCATC
    GCCCGCTGCA CTGGACCACC CGACATGGCT GACCGACTGC TGCTTACCAC CCCCCTCAAT
    CGCTTCCAGT GCAAAGGTCC AGCAGATCTG AATCTGATGT CCAAGTGCGC CCCCTGTCTG
    GCAACTCCCT GTCAGAATAA TGGCACTTGT GTGAGTGATG TCACAGGCTC CTACCACTGC
    ACCTGCCCTT TTGGCTACAA GGGTCGTAAC TGTGAGATTC CTATTAACGC CTGTATCAGC
    CTGCCATGTA CCAATGGAGG CACGTGTCAC CTCACACCTG GACTGGATGG ACAGTACAGC
    TGCGTGTGTC CTCCTGGGTA TGAGGGCCAG CAGTGTGAAC TGAATCCCGA TGACTGTGAA
    GACAACGATT GTGAGAATAA CTCCACCTGT GTGGATGGCA TCAACAACTA CACTTGTGTC
    TGCCCCCCTA ACTACAAAGG TGATCTGTGC GAGGAGGTGG TTGACCCCTG TCTGCCTGGG
    TTTGACCTCT GCCAACACGA CTCCAAGTGT GTTCCTCTCA GCAAGGGCTA CAGATGTGAG
    TGTTTGCCTG GTTATGTTGG CCAGCAGTGT GAGCAGGATT ACAATGACTG TCTGGAGAAC
    AAATGTCAGC ATGGGGCAGA GTGTGTTGAC GCCATCAATG GATACACATG TGTGTGCAAG
    GAGGGCTTCA GTGGGCTATT CTGTGAGAAT CATCCACCAA TGGTATTGCT CCAGAACACA
    AGTCCATGCG ATCAGTCGGA TTGTCAAAAT GGATCCGAAT GTCTGGTCAT GGAAGGAGAG
    CCAGTGTGCC GTTGTCTGCC TGGTTACTTC GGCACTAAGT GTCAAAAAAT GGTCACACTG
    CACTTTCTCG GCAAGGAAGC TTACGTGGAG CTCTCCGGCA CCAGGATCCG ACCACCCATG
    CACATTTCCT TCCAGGTAGC CACTGATAAG GATAATGGCA TTCTGTTGTA TAAAGAGGAC
    CATGACCCAC TCGCCCTAGA ACTGTACCAG GGACACATCC GTTTGATCTA TGACATTTCC
    AAATACCCCC CAACCACTGT GTACAGTGTG GAGTCAGTGA ATGATGGGCT TTTCCACACG
    GTGGAGGTGC TGATCCAGAA TCAGACACTG AGTTTGGTGG TGGATAAAGG GACTCCTAAA
    AGCTTGGGCA AACTCCCCCG TCCCCCTGCT GTGGACCACA ACACACAGCT ATACATAGGC
    GGTGTGCCAT CTCTGATTGG CACATCAGGC CTTCGGCCGG GCCCATTCCA GTCCTTCAAC
    GGCTGCCTTC ATGACGTACG CCTGAATGGA GAGCTCCAGG ACCTCGGTGC TGGACTGGCG
    GGGATAGAAG GCGTCCTGTT TGGGTGTCAC TCCTGCAGCG TCTGTGACCA GGGAGCCTGC
    AGACAAAGAG GAGACGAAGG GGTGACCTGT GACTGCCCCA CAGGACACAG CGGCCCACTA
    TGTGAACAGA TGAGCTCCCA AGACCCCTGT GAAAGCAGCA GGTGTGTTCA TGGGGAGTGT
    GTGGCGAAGG GCTCGTCGTA CAGCTGTTTA TGTGTGGATG GATACTCAGG CCAGTTCTGT
    GATAGACAGG ACGAGTCCAG CGCCTGCAAG GGACTGCACT GTGTTTATGG AGAGTGCAGA
    CTGACGGAGA GCGGAGAGCC CGCTTGTCAC TGCCAGCCTG GATACAGCGG GCCAACCTGC
    GAAACAGAAC TGACATGCGA AGGAGAAGCA GTTCGTGATT ATCTGTATCG CCAAATGGCT
    CATAAAATCT GCACGTCCAC CAGCAGAGTC CAGCGGCTGG AGTGTCGCCA CTCCTGCTCA
    TCTGGGCTGT GCTGTGCTCC CACCAAAAGC CGGAGACGGA AGCTCAGCTT CAAGTGTCCC
    GATGGATCAT CCTTTTCCGA GGAGATGGAG ATCACTGTGG AGTGTGGCTG CACAAAGTGC
    TCATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289318.1
    CDS227..4195
    Translation

    Target ORF information:

    RefSeq Version XM_009291043.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 3 (Drosophila) (slit3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291043.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCCAAAGC TGCGGACACT TCGCCTGCAC TCCAACAACC TGCACTGCGA CTGCCACCTG 
    TCCTGGCTCT CTGATTGGCT GCGGCAGAGG AGGGGTTTGG CCCCTTTCAC ACAGTGCATG
    GCCCCCGCCC ATATGAGAGG TCTCAATGTC CCTGATGTTC AAAAGAGAGA ATTTGTTTGC
    ACAGGTCCCG TAGAGACGGA GCCAAGGTCA TGTGCTCCGC AGGTGACCAT CTGCCCTGCC
    GCCTGCACCT GCAACAACAA CATTGTGGAC TGCCGTAGAA AAGGCCTGAC TGAGATTCCT
    GCCAACCTGC CTGAAGGAAT CGTGGAAATC CGTCTGGAGC AGAACATGAT CAAGAACATC
    CCAGCAGGAG CCTTCTCCAC ATACAAGAAA CTCAAGAGAA TAGACCTGAG CAAGAATCAG
    ATATCCGAAA TTGCAGAAGA CGCATTTAGT GGCCTGCGCT CTCTCACGTC ACTGGTCCTG
    TACGGAAATA AAATCGCAGA GATCCCAAAG GGACTGTTTG ATGGCCTGGT CTCCCTGCAA
    CTGCTACTGC TGAACGCCAA CAAGATCAAC TGCCTTCGAG TGAACACTTT CAAAGATCTG
    CAGAACCTCA ACCTGCTGTC CCTCTATGAC AACAAACTTC AGACCATCAG CAAAGGCTTG
    TTCGCTCCTC TGCGTGCCAT CAAAACCCTA CACCTCGCTC AGAATCCGTT CATGTGTGAC
    TGTCATCTGA AGTGGCTGGC CGACTACCTG TTTGACAATC CAATTGAGAC CAGCGGGGCT
    CGATGCAGCC ACCCGCGCCG CCTCGCCAAC AAGCGCATCA GCCAGGTCAA GGGCAAGAAG
    TTTCGCTGCA CAGGTGCGGA GGACTACCGC AGTCGTCTCA GCGGCGAGTG TTTCCAAGAC
    CTGGTTTGTC CCGAACGCTG CCGATGTGAA GGAACAGTAG TGGACTGCTC CAACCTCAAA
    CTCACCAAAA TCCCCCCTCA CCTTCCTGAG CACACCACTG ACCTACGGCT GAACGACAAT
    GAAATCTCCA TCTTAGAGGC TACAGGAGCA TTCAAGAAGC TTCCAAATCT GCGCAAGATA
    AACTTGAGCA ATAATAAACT CCGGGACATC CGTGAGGGTG CGTTTGATGG GGCCGGAGGT
    GTTTTGGAGC TGCTGTTGAC TGGAAACAAG CTGCAGTCGG TCAGCGGACG GATGTTCAAG
    GGTTTGATCG GCCTCAAAAC ACTGATGCTG AGGAGTAATC AGATCAGCTG TGTGGATAAC
    GCCACCTTCA CCGGCTTGAG CTCTGTGCGC CTGCTTTCCC TCTACGATAA CCGGATCTCC
    ACTATCGCCC CCGGAGCTTT CAACACCCTG CATTCCCTTT CCACCATTAA CCTGCTGTCT
    AATCCCTACG TGTGTGACTG TCACTTAGCC TGGCTGGGGC TGTGGCTTAA GAAGACCAGG
    GTGGTTAGCG GAAACCCACG CTGTCAGAAA CCTGCCTTCC TGAAGGAGAT CCCTATTCAA
    GATGTGGCCA TGCCTGATTT CTCCTGTGAT GGTGCGGAAG AGAATGTGTG CCTGCCGTCA
    GCCCCTCGAT GTCCAGAATC CTGCACATGC TCGGAAACCG TGGTGCGCTG TAGTAACCGT
    GGGCTGCGTT CTCTGCCTAA GACTATCCCT AAAGATACAA CTGAACTATA CTTGGAAGGA
    AACCTCCTCA CATCCGTTCC CAAAGAGCTT CCTAATCTGA AACAACTGAC ACTCCTAGAC
    CTTAGTAATA ACAGCATCAG TCATCTTGCC CCTCTCACCT TCAGCAACAT GACACAGCTC
    GCCACCCTTA TCCTGAGCTA TAATCAAATA CGCTGCGTCC CGGTTCATGC GTTTGATGGC
    TTGCGCTCTC TCCGCCTGCT AACTCTCCAC GGAAATGACC TTTCAACTGT TCCGGAGGGA
    GCCTTCAACC ACTTGACATC TCTATCTCAT CTAGCTCTCG GTGCGAACCC TTTATACTGT
    GACTGTGAGC TGCGCTGGTT GTCTCAGTGG GTGAAGGCTG GCTTTAAAGA GCCTGGCATC
    GCCCGCTGCA CTGGACCACC CGACATGGCT GACCGACTGC TGCTTACCAC CCCCCTCAAT
    CGCTTCCAGT GCAAAGGTCC AGCAGATCTG AATCTGATGT CCAAGTGCGC CCCCTGTCTG
    GCAACTCCCT GTCAGAATAA TGGCACTTGT GTGAGTGATG TCACAGGCTC CTACCACTGC
    ACCTGCCCTT TTGGCTACAA GGGTCGTAAC TGTGAGATTC CTATTAACGC CTGTATCAGC
    CTGCCATGTA CCAATGGAGG CACGTGTCAC CTCACACCTG GACTGGATGG ACAGTACAGC
    TGCGTGTGTC CTCCTGGGTA TGAGGGCCAG CAGTGTGAAC TGAATCCCGA TGACTGTGAA
    GACAACGATT GTGAGAATAA CTCCACCTGT GTGGATGGCA TCAACAACTA CACTTGTGTC
    TGCCCCCCTA ACTACAAAGG TGATCTGTGC GAGGAGGTGG TTGACCCCTG TCTGCCTGGG
    TTTGACCTCT GCCAACACGA CTCCAAGTGT GTTCCTCTCA GCAAGGGCTA CAGATGTGAG
    TGTTTGCCTG GTTATGTTGG CCAGCAGTGT GAGCAGGATT ACAATGACTG TCTGGAGAAC
    AAATGTCAGC ATGGGGCAGA GTGTGTTGAC GCCATCAATG GATACACATG TGTGTGCAAG
    GAGGGCTTCA GTGGGCTATT CTGTGAGAAT CATCCACCAA TGGTATTGCT CCAGAACACA
    AGTCCATGCG ATCAGTCGGA TTGTCAAAAT GGATCCGAAT GTCTGGTCAT GGAAGGAGAG
    CCAGTGTGCC GTTGTCTGCC TGGTTACTTC GGCACTAAGT GTCAAAAAAT GGTCACACTG
    CACTTTCTCG GCAAGGAAGC TTACGTGGAG CTCTCCGGCA CCAGGATCCG ACCACCCATG
    CACATTTCCT TCCAGGTAGC CACTGATAAG GATAATGGCA TTCTGTTGTA TAAAGAGGAC
    CATGACCCAC TCGCCCTAGA ACTGTACCAG GGACACATCC GTTTGATCTA TGACATTTCC
    AAATACCCCC CAACCACTGT GTACAGTGTG GAGTCAGTGA ATGATGGGCT TTTCCACACG
    GTGGAGGTGC TGATCCAGAA TCAGACACTG AGTTTGGTGG TGGATAAAGG GACTCCTAAA
    AGCTTGGGCA AACTCCCCCG TCCCCCTGCT GTGGACCACA ACACACAGCT ATACATAGGC
    GGTGTGCCAT CTCTGATTGG CACATCAGGC CTTCGGCCGG GCCCATTCCA GTCCTTCAAC
    GGCTGCCTTC ATGACGTACG CCTGAATGGA GAGCTCCAGG ACCTCGGTGC TGGACTGGCG
    GGGATAGAAG GCGTCCTGTT TGGGTGTCAC TCCTGCAGCG TCTGTGACCA GGGAGCCTGC
    AGACAAAGAG GAGACGAAGG GGTGACCTGT GACTGCCCCA CAGGACACAG CGGCCCACTA
    TGTGAACAGA TGAGCTCCCA AGACCCCTGT GAAAGCAGCA GGTGTGTTCA TGGGGAGTGT
    GTGGCGAAGG GCTCGTCGTA CAGCTGTTTA TGTGTGGATG GATACTCAGG CCAGTTCTGT
    GATAGACAGG ACGAGTCCAG CGCCTGCAAG GGACTGCACT GTGTTTATGG AGAGTGCAGA
    CTGACGGAGA GCGGAGAGCC CGCTTGTCAC TGCCAGCCTG GATACAGCGG GCCAACCTGC
    GAAACAGAAC TGACATGCGA AGGAGAAGCA GTTCGTGATT ATCTGTATCG CCAAATGGCT
    CATAAAATCT GCACGTCCAC CAGCAGAGTC CAGCGGCTGG AGTGTCGCCA CTCCTGCTCA
    TCTGGGCTGT GCTGTGCTCC CACCAAAAGC CGGAGACGGA AGCTCAGCTT CAAGTGTCCC
    GATGGATCAT CCTTTTCCGA GGAGATGGAG ATCACTGTGG AGTGTGGCTG CACAAAGTGC
    TCATCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60483
    Clone ID Related Accession (Same CDS sequence) NM_131736.3
    Accession Version NM_131736.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-18
    Organism Danio rerio(zebrafish)
    Product slit homolog 3 protein precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL928691.7 and BX323583.36. On Oct 29, 2015 this sequence version replaced NM_131736.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC139487.1, GDQH01012275.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGCGGT GGGTAAAGTA TTGGGGGACA GTGGCTGTGC TCTGCGGGCT GCTGAGCTGT 
    GCTGTGAATG GATGCCCGCA TAAGTGCAGT TGTTCGGGCT CTCATGTGGA TTGTCAGGGT
    CAAGCCTTCA AGACGGTGCC TAGGGGAATC CCGAGGAATG CCGAGAGACT GGATCTGGAC
    AGGAATAACA TCACCCGGAT CACCAAAGTG GACTTCTCTG GCCTCAAGAA CCTGCGCGTG
    CTACATCTAG AGAACAATCA AATAAGTGTG ATTGAAAGAG GAGCATTTGA GGGTCTCAAA
    CAACTTGAGA GAATCCGCCT GAACCGGAAC CGGCTTCAGG TCCTGCCGGA GCTGCTGTTC
    CAATCCACAA CCAAGCTGAG CCGCCTGGAC CTGAGCGAGA ATCAGATTCA GGCTGTTCCT
    CGTAAGGCTT TCCGAGGGAT CACCACTGTC AAGAACCTGC AACTGGACAG CAACCACATC
    AGCTGCATCG AAGATGGAGC CTTCCGAGCC CTGCGCGATT TGGAGATACT CACTCTCAAC
    AACAACAATA TCACCCTCAT CCCACTGTCA AGCTTCAACC ACATGCCAAA GCTGCGGACA
    CTTCGCCTGC ACTCCAACAA CCTGCACTGC GACTGCCACC TGTCCTGGCT CTCTGATTGG
    CTGCGGCAGA GGAGGGGTTT GGCCCCTTTC ACACAGTGCA TGGCCCCCGC CCATATGAGA
    GGTCTCAATG TCCCTGATGT TCAAAAGAGA GAATTTGTTT GCACAGGTCC CGTAGAGACG
    GAGCCAAGGT CATGTGCTCC GCAGGTGACC ATCTGCCCTG CCGCCTGCAC CTGCAACAAC
    AACATTGTGG ACTGCCGTAG AAAAGGCCTG ACTGAGATTC CTGCCAACCT GCCTGAAGGA
    ATCGTGGAAA TCCGTCTGGA GCAGAACATG ATCAAGAACA TCCCAGCAGG AGCCTTCTCC
    ACATACAAGA AACTCAAGAG AATAGACCTG AGCAAGAATC AGATATCCGA AATTGCAGAA
    GACGCATTTA GTGGCCTGCG CTCTCTCACG TCACTGGTCC TGTACGGAAA TAAAATCGCA
    GAGATCCCAA AGGGACTGTT TGATGGCCTG GTCTCCCTGC AACTGCTACT GCTGAACGCC
    AACAAGATCA ACTGCCTTCG AGTGAACACT TTCAAAGATC TGCAGAACCT CAACCTGCTG
    TCCCTCTATG ACAACAAACT TCAGACCATC AGCAAAGGCT TGTTCGCTCC TCTGCGTGCC
    ATCAAAACCC TACACCTCGC TCAGAATCCG TTCATGTGTG ACTGTCATCT GAAGTGGCTG
    GCCGACTACC TGTTTGACAA TCCAATTGAG ACCAGCGGGG CTCGATGCAG CCACCCGCGC
    CGCCTCGCCA ACAAGCGCAT CAGCCAGGTC AAGGGCAAGA AGTTTCGCTG CACAGGTGCG
    GAGGACTACC GCAGTCGTCT CAGCGGCGAG TGTTTCCAAG ACCTGGTTTG TCCCGAACGC
    TGCCGATGTG AAGGAACAGT AGTGGACTGC TCCAACCTCA AACTCACCAA AATCCCCCCT
    CACCTTCCTG AGCACACCAC TGACCTACGG CTGAACGACA ATGAAATCTC CATCTTAGAG
    GCTACAGGAG CATTCAAGAA GCTTCCAAAT CTGCGCAAGA TAAACTTGAG CAATAATAAA
    CTCCGGGACA TCCGTGAGGG TGCGTTTGAT GGGGCCGGAG GTGTTTTGGA GCTGCTGTTG
    ACTGGAAACA AGCTGCAGTC GGTCAGCGGA CGGATGTTCA AGGGTTTGAT CGGCCTCAAA
    ACACTGATGC TGAGGAGTAA TCAGATCAGC TGTGTGGATA ACGCCACCTT CACCGGCTTG
    AGCTCTGTGC GCCTGCTTTC CCTCTACGAT AACCGGATCT CCACTATCGC CCCCGGAGCT
    TTCAACACCC TGCATTCCCT TTCCACCATT AACCTGCTGT CTAATCCCTA CGTGTGTGAC
    TGTCACTTAG CCTGGCTGGG GCTGTGGCTT AAGAAGACCA GGGTGGTTAG CGGAAACCCA
    CGCTGTCAGA AACCTGCCTT CCTGAAGGAG ATCCCTATTC AAGATGTGGC CATGCCTGAT
    TTCTCCTGTG ATGGTGCGGA AGAGAATGTG TGCCTGCCGT CAGCCCCTCG ATGTCCAGAA
    TCCTGCACAT GCTCGGAAAC CGTGGTGCGC TGTAGTAACC GTGGGCTGCG TTCTCTGCCT
    AAGACTATCC CTAAAGATAC AACTGAACTA TACTTGGAAG GAAACCTCCT CACATCCGTT
    CCCAAAGAGC TTCCTAATCT GAAACAACTG ACACTCCTAG ACCTTAGTAA TAACAGCATC
    AGTCATCTTG CCCCTCTCAC CTTCAGCAAC ATGACACAGC TCGCCACCCT TATCCTGAGC
    TATAATCAAA TACGCTGCGT CCCGGTTCAT GCGTTTGATG GCTTGCGCTC TCTCCGCCTG
    CTAACTCTCC ACGGAAATGA CCTTTCAACT GTTCCGGAGG GAGCCTTCAA CCACTTGACA
    TCTCTATCTC ATCTAGCTCT CGGTGCGAAC CCTTTATACT GTGACTGTGA GCTGCGCTGG
    TTGTCTCAGT GGGTGAAGGC TGGCTTTAAA GAGCCTGGCA TCGCCCGCTG CACTGGACCA
    CCCGACATGG CTGACCGACT GCTGCTTACC ACCCCCCTCA ATCGCTTCCA GTGCAAAGGT
    CCAGCAGATC TGAATCTGAT GTCCAAGTGC GCCCCCTGTC TGGCAACTCC CTGTCAGAAT
    AATGGCACTT GTGTGAGTGA TGTCACAGGC TCCTACCACT GCACCTGCCC TTTTGGCTAC
    AAGGGTCGTA ACTGTGAGAT TCCTATTAAC GCCTGTATCA GCCTGCCATG TACCAATGGA
    GGCACGTGTC ACCTCACACC TGGACTGGAT GGACAGTACA GCTGCGTGTG TCCTCCTGGG
    TATGAGGGCC AGCAGTGTGA ACTGAATCCC GATGACTGTG AAGACAACGA TTGTGAGAAT
    AACTCCACCT GTGTGGATGG CATCAACAAC TACACTTGTG TCTGCCCCCC TAACTACAAA
    GGTGATCTGT GCGAGGAGGT GGTTGACCCC TGTCTGCCTG GGTTTGACCT CTGCCAACAC
    GACTCCAAGT GTGTTCCTCT CAGCAAGGGC TACAGATGTG AGTGTTTGCC TGGTTATGTT
    GGCCAGCAGT GTGAGCAGGA TTACAATGAC TGTCTGGAGA ACAAATGTCA GCATGGGGCA
    GAGTGTGTTG ACGCCATCAA TGGATACACA TGTGTGTGCA AGGAGGGCTT CAGTGGGCTA
    TTCTGTGAGA ATCATCCACC AATGGTATTG CTCCAGAACA CAAGTCCATG CGATCAGTCG
    GATTGTCAAA ATGGATCCGA ATGTCTGGTC ATGGAAGGAG AGCCAGTGTG CCGTTGTCTG
    CCTGGTTACT TCGGCACTAA GTGTCAAAAA ATGGTCACAC TGCACTTTCT CGGCAAGGAA
    GCTTACGTGG AGCTCTCCGG CACCAGGATC CGACCACCCA TGCACATTTC CTTCCAGGTA
    GCCACTGATA AGGATAATGG CATTCTGTTG TATAAAGAGG ACCATGACCC ACTCGCCCTA
    GAACTGTACC AGGGACACAT CCGTTTGATC TATGACATTT CCAAATACCC CCCAACCACT
    GTGTACAGTG TGGAGTCAGT GAATGATGGG CTTTTCCACA CGGTGGAGGT GCTGATCCAG
    AATCAGACAC TGAGTTTGGT GGTGGATAAA GGGACTCCTA AAAGCTTGGG CAAACTCCCC
    CGTCCCCCTG CTGTGGACCA CAACACACAG CTATACATAG GCGGTGTGCC ATCTCTGATT
    GGCACATCAG GCCTTCGGCC GGGCCCATTC CAGTCCTTCA ACGGCTGCCT TCATGACGTA
    CGCCTGAATG GAGAGCTCCA GGACCTCGGT GCTGGACTGG CGGGGATAGA AGGCGTCCTG
    TTTGGGTGTC ACTCCTGCAG CGTCTGTGAC CAGGGAGCCT GCAGACAAAG AGGAGACGAA
    GGGGTGACCT GTGACTGCCC CACAGGACAC AGCGGCCCAC TATGTGAACA GATGAGCTCC
    CAAGACCCCT GTGAAAGCAG CAGGTGTGTT CATGGGGAGT GTGTGGCGAA GGGCTCGTCG
    TACAGCTGTT TATGTGTGGA TGGATACTCA GGCCAGTTCT GTGATAGACA GGACGAGTCC
    AGCGCCTGCA AGGGACTGCA CTGTGTTTAT GGAGAGTGCA GACTGACGGA GAGCGGAGAG
    CCCGCTTGTC ACTGCCAGCC TGGATACAGC GGGCCAACCT GCGAAACAGA ACTGACATGC
    GAAGGAGAAG CAGTTCGTGA TTATCTGTAT CGCCAAATGG CTCATAAAAT CTGCACGTCC
    ACCAGCAGAG TCCAGCGGCT GGAGTGTCGC CACTCCTGCT CATCTGGGCT GTGCTGTGCT
    CCCACCAAAA GCCGGAGACG GAAGCTCAGC TTCAAGTGTC CCGATGGATC ATCCTTTTCC
    GAGGAGATGG AGATCACTGT GGAGTGTGGC TGCACAAAGT GCTCATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_571811.3
    CDS430..4980
    Translation

    Target ORF information:

    RefSeq Version NM_131736.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 3 (Drosophila) (slit3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_131736.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGCGGT GGGTAAAGTA TTGGGGGACA GTGGCTGTGC TCTGCGGGCT GCTGAGCTGT 
    GCTGTGAATG GATGCCCGCA TAAGTGCAGT TGTTCGGGCT CTCATGTGGA TTGTCAGGGT
    CAAGCCTTCA AGACGGTGCC TAGGGGAATC CCGAGGAATG CCGAGAGACT GGATCTGGAC
    AGGAATAACA TCACCCGGAT CACCAAAGTG GACTTCTCTG GCCTCAAGAA CCTGCGCGTG
    CTACATCTAG AGAACAATCA AATAAGTGTG ATTGAAAGAG GAGCATTTGA GGGTCTCAAA
    CAACTTGAGA GAATCCGCCT GAACCGGAAC CGGCTTCAGG TCCTGCCGGA GCTGCTGTTC
    CAATCCACAA CCAAGCTGAG CCGCCTGGAC CTGAGCGAGA ATCAGATTCA GGCTGTTCCT
    CGTAAGGCTT TCCGAGGGAT CACCACTGTC AAGAACCTGC AACTGGACAG CAACCACATC
    AGCTGCATCG AAGATGGAGC CTTCCGAGCC CTGCGCGATT TGGAGATACT CACTCTCAAC
    AACAACAATA TCACCCTCAT CCCACTGTCA AGCTTCAACC ACATGCCAAA GCTGCGGACA
    CTTCGCCTGC ACTCCAACAA CCTGCACTGC GACTGCCACC TGTCCTGGCT CTCTGATTGG
    CTGCGGCAGA GGAGGGGTTT GGCCCCTTTC ACACAGTGCA TGGCCCCCGC CCATATGAGA
    GGTCTCAATG TCCCTGATGT TCAAAAGAGA GAATTTGTTT GCACAGGTCC CGTAGAGACG
    GAGCCAAGGT CATGTGCTCC GCAGGTGACC ATCTGCCCTG CCGCCTGCAC CTGCAACAAC
    AACATTGTGG ACTGCCGTAG AAAAGGCCTG ACTGAGATTC CTGCCAACCT GCCTGAAGGA
    ATCGTGGAAA TCCGTCTGGA GCAGAACATG ATCAAGAACA TCCCAGCAGG AGCCTTCTCC
    ACATACAAGA AACTCAAGAG AATAGACCTG AGCAAGAATC AGATATCCGA AATTGCAGAA
    GACGCATTTA GTGGCCTGCG CTCTCTCACG TCACTGGTCC TGTACGGAAA TAAAATCGCA
    GAGATCCCAA AGGGACTGTT TGATGGCCTG GTCTCCCTGC AACTGCTACT GCTGAACGCC
    AACAAGATCA ACTGCCTTCG AGTGAACACT TTCAAAGATC TGCAGAACCT CAACCTGCTG
    TCCCTCTATG ACAACAAACT TCAGACCATC AGCAAAGGCT TGTTCGCTCC TCTGCGTGCC
    ATCAAAACCC TACACCTCGC TCAGAATCCG TTCATGTGTG ACTGTCATCT GAAGTGGCTG
    GCCGACTACC TGTTTGACAA TCCAATTGAG ACCAGCGGGG CTCGATGCAG CCACCCGCGC
    CGCCTCGCCA ACAAGCGCAT CAGCCAGGTC AAGGGCAAGA AGTTTCGCTG CACAGGTGCG
    GAGGACTACC GCAGTCGTCT CAGCGGCGAG TGTTTCCAAG ACCTGGTTTG TCCCGAACGC
    TGCCGATGTG AAGGAACAGT AGTGGACTGC TCCAACCTCA AACTCACCAA AATCCCCCCT
    CACCTTCCTG AGCACACCAC TGACCTACGG CTGAACGACA ATGAAATCTC CATCTTAGAG
    GCTACAGGAG CATTCAAGAA GCTTCCAAAT CTGCGCAAGA TAAACTTGAG CAATAATAAA
    CTCCGGGACA TCCGTGAGGG TGCGTTTGAT GGGGCCGGAG GTGTTTTGGA GCTGCTGTTG
    ACTGGAAACA AGCTGCAGTC GGTCAGCGGA CGGATGTTCA AGGGTTTGAT CGGCCTCAAA
    ACACTGATGC TGAGGAGTAA TCAGATCAGC TGTGTGGATA ACGCCACCTT CACCGGCTTG
    AGCTCTGTGC GCCTGCTTTC CCTCTACGAT AACCGGATCT CCACTATCGC CCCCGGAGCT
    TTCAACACCC TGCATTCCCT TTCCACCATT AACCTGCTGT CTAATCCCTA CGTGTGTGAC
    TGTCACTTAG CCTGGCTGGG GCTGTGGCTT AAGAAGACCA GGGTGGTTAG CGGAAACCCA
    CGCTGTCAGA AACCTGCCTT CCTGAAGGAG ATCCCTATTC AAGATGTGGC CATGCCTGAT
    TTCTCCTGTG ATGGTGCGGA AGAGAATGTG TGCCTGCCGT CAGCCCCTCG ATGTCCAGAA
    TCCTGCACAT GCTCGGAAAC CGTGGTGCGC TGTAGTAACC GTGGGCTGCG TTCTCTGCCT
    AAGACTATCC CTAAAGATAC AACTGAACTA TACTTGGAAG GAAACCTCCT CACATCCGTT
    CCCAAAGAGC TTCCTAATCT GAAACAACTG ACACTCCTAG ACCTTAGTAA TAACAGCATC
    AGTCATCTTG CCCCTCTCAC CTTCAGCAAC ATGACACAGC TCGCCACCCT TATCCTGAGC
    TATAATCAAA TACGCTGCGT CCCGGTTCAT GCGTTTGATG GCTTGCGCTC TCTCCGCCTG
    CTAACTCTCC ACGGAAATGA CCTTTCAACT GTTCCGGAGG GAGCCTTCAA CCACTTGACA
    TCTCTATCTC ATCTAGCTCT CGGTGCGAAC CCTTTATACT GTGACTGTGA GCTGCGCTGG
    TTGTCTCAGT GGGTGAAGGC TGGCTTTAAA GAGCCTGGCA TCGCCCGCTG CACTGGACCA
    CCCGACATGG CTGACCGACT GCTGCTTACC ACCCCCCTCA ATCGCTTCCA GTGCAAAGGT
    CCAGCAGATC TGAATCTGAT GTCCAAGTGC GCCCCCTGTC TGGCAACTCC CTGTCAGAAT
    AATGGCACTT GTGTGAGTGA TGTCACAGGC TCCTACCACT GCACCTGCCC TTTTGGCTAC
    AAGGGTCGTA ACTGTGAGAT TCCTATTAAC GCCTGTATCA GCCTGCCATG TACCAATGGA
    GGCACGTGTC ACCTCACACC TGGACTGGAT GGACAGTACA GCTGCGTGTG TCCTCCTGGG
    TATGAGGGCC AGCAGTGTGA ACTGAATCCC GATGACTGTG AAGACAACGA TTGTGAGAAT
    AACTCCACCT GTGTGGATGG CATCAACAAC TACACTTGTG TCTGCCCCCC TAACTACAAA
    GGTGATCTGT GCGAGGAGGT GGTTGACCCC TGTCTGCCTG GGTTTGACCT CTGCCAACAC
    GACTCCAAGT GTGTTCCTCT CAGCAAGGGC TACAGATGTG AGTGTTTGCC TGGTTATGTT
    GGCCAGCAGT GTGAGCAGGA TTACAATGAC TGTCTGGAGA ACAAATGTCA GCATGGGGCA
    GAGTGTGTTG ACGCCATCAA TGGATACACA TGTGTGTGCA AGGAGGGCTT CAGTGGGCTA
    TTCTGTGAGA ATCATCCACC AATGGTATTG CTCCAGAACA CAAGTCCATG CGATCAGTCG
    GATTGTCAAA ATGGATCCGA ATGTCTGGTC ATGGAAGGAG AGCCAGTGTG CCGTTGTCTG
    CCTGGTTACT TCGGCACTAA GTGTCAAAAA ATGGTCACAC TGCACTTTCT CGGCAAGGAA
    GCTTACGTGG AGCTCTCCGG CACCAGGATC CGACCACCCA TGCACATTTC CTTCCAGGTA
    GCCACTGATA AGGATAATGG CATTCTGTTG TATAAAGAGG ACCATGACCC ACTCGCCCTA
    GAACTGTACC AGGGACACAT CCGTTTGATC TATGACATTT CCAAATACCC CCCAACCACT
    GTGTACAGTG TGGAGTCAGT GAATGATGGG CTTTTCCACA CGGTGGAGGT GCTGATCCAG
    AATCAGACAC TGAGTTTGGT GGTGGATAAA GGGACTCCTA AAAGCTTGGG CAAACTCCCC
    CGTCCCCCTG CTGTGGACCA CAACACACAG CTATACATAG GCGGTGTGCC ATCTCTGATT
    GGCACATCAG GCCTTCGGCC GGGCCCATTC CAGTCCTTCA ACGGCTGCCT TCATGACGTA
    CGCCTGAATG GAGAGCTCCA GGACCTCGGT GCTGGACTGG CGGGGATAGA AGGCGTCCTG
    TTTGGGTGTC ACTCCTGCAG CGTCTGTGAC CAGGGAGCCT GCAGACAAAG AGGAGACGAA
    GGGGTGACCT GTGACTGCCC CACAGGACAC AGCGGCCCAC TATGTGAACA GATGAGCTCC
    CAAGACCCCT GTGAAAGCAG CAGGTGTGTT CATGGGGAGT GTGTGGCGAA GGGCTCGTCG
    TACAGCTGTT TATGTGTGGA TGGATACTCA GGCCAGTTCT GTGATAGACA GGACGAGTCC
    AGCGCCTGCA AGGGACTGCA CTGTGTTTAT GGAGAGTGCA GACTGACGGA GAGCGGAGAG
    CCCGCTTGTC ACTGCCAGCC TGGATACAGC GGGCCAACCT GCGAAACAGA ACTGACATGC
    GAAGGAGAAG CAGTTCGTGA TTATCTGTAT CGCCAAATGG CTCATAAAAT CTGCACGTCC
    ACCAGCAGAG TCCAGCGGCT GGAGTGTCGC CACTCCTGCT CATCTGGGCT GTGCTGTGCT
    CCCACCAAAA GCCGGAGACG GAAGCTCAGC TTCAAGTGTC CCGATGGATC ATCCTTTTCC
    GAGGAGATGG AGATCACTGT GGAGTGTGGC TGCACAAAGT GCTCATCGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.