UPF3B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UPF3B
Entrez Gene ID	65109
Full Name	UPF3B, regulator of nonsense mediated mRNA decay
Synonyms	HUPF3B,MRX62,MRXS14,RENT3B,UPF3BP1,UPF3BP2,UPF3BP3,UPF3X,Upf3p-X
General protein information	Preferred Names UPF3B, regulator of nonsense mediated mRNA decay Names regulator of nonsense transcripts 3B UPF3 regulator of nonsense transcripts homolog B UPF3B pseudogene 1 UPF3B pseudogene 2 UPF3B pseudogene 3 mental retardation, X-linked 62 nonsense mRNA reducing factor 3B up-frameshift suppressor 3 homolog B up-frameshift suppressor 3 homolog on chromosome X
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq24
Summary	This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	300298
Disorder Html:	Mental retardation, X-linked, syndromic 14, 300676 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017029737.1	XP_016885226.1	regulator of nonsense transcripts 3B isoform X1
XM_017029738.1	XP_016885227.1	regulator of nonsense transcripts 3B isoform X2
XM_017029739.1	XP_016885228.1	regulator of nonsense transcripts 3B isoform X3
NM_023010.3	NP_075386.1	regulator of nonsense transcripts 3B isoform 2
NM_080632.2	NP_542199.1	regulator of nonsense transcripts 3B isoform 1
NM_080632.3	NP_542199.1	regulator of nonsense transcripts 3B isoform 1
XM_017029740.1	XP_016885229.1	regulator of nonsense transcripts 3B isoform X4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Upf3b	NP_080849.1
Homo sapiens (human)	UPF3B	NP_542199.1
	UPF3B	XP_001083072.1
Rattus norvegicus (Norway rat)	Upf3b	NP_001129345.1
Gallus gallus (chicken)	UPF3B	XP_003641170.1
Xenopus tropicalis (tropical clawed frog)	upf3b	XP_002940330.1
Pan troglodytes (chimpanzee)	UPF3B	XP_003954560.1
Canis lupus familiaris (dog)	UPF3B	XP_851843.1
Danio rerio (zebrafish)	upf3b	NP_957248.1

Gene Ontology
Bibliography

Related articles in PubMed

Dual function of UPF3B in early and late translation termination.
Neu-Yilik G, Raimondeau E, Eliseev B, Yeramala L, Amthor B, Deniaud A, Huard K, Kerschgens K, Hentze MW, Schaffitzel C, Kulozik AE
The EMBO journal36(20)2968-2986(2017 10)

Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex.
Buchwald G, Ebert J, Basquin C, Sauliere J, Jayachandran U, Bono F, Le Hir H, Conti E
Proceedings of the National Academy of Sciences of the United States of America107(22)10050-5(2010 Jun)

A UPF3-mediated regulatory switch that maintains RNA surveillance.
Chan WK, Bhalla AD, Le Hir H, Nguyen LS, Huang L, G?cz J, Wilkinson MF
Nature structural & molecular biology16(7)747-53(2009 Jul)

Nonsense mediated decay induced by tethered human UPF3B is restricted to the cytoplasm.
Lu S, Cullen BR
RNA biology1(1)42-7(2004 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors discovered that UPF3B (i) interacts with the release factors, (ii) delays translation termination and (iii) dissociates post-termination ribosomal complexes that are devoid of the nascent peptide.
Title: Dual function of UPF3B in early and late translation termination.

UPF3B gene mutation is associated with Lujan-Fryns syndrome.
Title: Tentative clinical diagnosis of Lujan-Fryns syndrome--A conglomeration of different genetic entities?

the neurodevelopmental phenotype of UPF3B missense mutation is caused by impairment of nonsense-mediated mRNA decay pathway function altering neuronal differentiation.
Title: Full UPF3B function is critical for neuronal differentiation of neural stem cells.

These findings indicate that SATB2 activates UPF3B expression through binding to its promoter.
Title: Disorders with similar clinical phenotypes reveal underlying genetic interaction: SATB2 acts as an activator of the UPF3B gene.

Data indicate the mutation p.R430X of UPF3B gene as the genetic etiology in the mental retardation pedigree.
Title: Exome sequencing identifies UPF3B as the causative gene for a Chinese non-syndrome mental retardation pedigree.

The following UPF3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UPF3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03963	NM_023010.3 Latest version!	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu18945	NM_080632.3 Latest version!	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu99427	XM_017029737.1 Latest version!	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu99428	XM_017029738.1 Latest version!	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu99429	XM_017029739.1 Latest version!	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu99430	XM_017029740.1 Latest version!	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu18945	NM_080632.2	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UPF3B ( NM_023010.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_075386.1 Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03963
Clone ID Related Accession (Same CDS sequence)	NM_023010.3
Accession Version	NM_023010.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 3B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA949746.1, BC121017.1 and AY013251.1. On Aug 21, 2010 this sequence version replaced NM_023010.2. Transcript Variant: This variant (2) lacks an alternate coding segment, compared to variant 1, and encodes the shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY013251.1, SRR1803612.192324.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGCTG CTCAAGAAGC CAGAAAAAGG AGATGAAAAA 
GAATTGGACA AAAGAGAAAA AGCCAAGAAA TTGGACAAAG AGAATCTCAG TGATGAAAGA 
GCCAGTGGGC AAAGTTGTAC ATTGCCCAAG CGTTCTGATA GCGAACTTAA AGATGAAAAA 
CCAAAGAGAC CTGAAGATGA GAGCGGCAGA GACTATAGGG AGAGGGAACG GGAATATGAA 
CGAGATCAGG AGCGCATACT TCGAGAAAGA GAGAGGCTGA AGCGGCAAGA AGAAGAGCGC 
CGTAGGCAGA AGGAGCGCTA TGAGAAAGAG AAGACTTTTA AGAGAAAAGA AGAAGAAATG 
AAAAAAGAGA AAGACACACT TCGGGATAAA GGAAAGAAGG CTGAAAGTAC AGAATCAATA 
GGCAGCTCAG AAAAAACTGA AAAGAAAGAA GAAGTGGTCA AGAGAGATCG AATAAGAAAC 
AAGGATCGTC CAGCGATGCA GCTTTACCAA CCAGGAGCTC GAAGCCGAAA TCGACTCTGT 
CCCCCTGATG ACAGCACCAA GTCTGGAGAT TCAGCAGCAG AAAGGAAGCA GGAAAGTGGT 
ATTAGCCATA GAAAAGAAGG AGGAGAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075386.1
CDS	101..1513
Translation	MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE

Target ORF information:

RefSeq Version	NM_023010.3
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023010.3

ORF Insert Sequence:

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGCTG CTCAAGAAGC CAGAAAAAGG AGATGAAAAA 
GAATTGGACA AAAGAGAAAA AGCCAAGAAA TTGGACAAAG AGAATCTCAG TGATGAAAGA 
GCCAGTGGGC AAAGTTGTAC ATTGCCCAAG CGTTCTGATA GCGAACTTAA AGATGAAAAA 
CCAAAGAGAC CTGAAGATGA GAGCGGCAGA GACTATAGGG AGAGGGAACG GGAATATGAA 
CGAGATCAGG AGCGCATACT TCGAGAAAGA GAGAGGCTGA AGCGGCAAGA AGAAGAGCGC 
CGTAGGCAGA AGGAGCGCTA TGAGAAAGAG AAGACTTTTA AGAGAAAAGA AGAAGAAATG 
AAAAAAGAGA AAGACACACT TCGGGATAAA GGAAAGAAGG CTGAAAGTAC AGAATCAATA 
GGCAGCTCAG AAAAAACTGA AAAGAAAGAA GAAGTGGTCA AGAGAGATCG AATAAGAAAC 
AAGGATCGTC CAGCGATGCA GCTTTACCAA CCAGGAGCTC GAAGCCGAAA TCGACTCTGT 
CCCCCTGATG ACAGCACCAA GTCTGGAGAT TCAGCAGCAG AAAGGAAGCA GGAAAGTGGT 
ATTAGCCATA GAAAAGAAGG AGGAGAGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF3B ( NM_080632.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_542199.1 Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18945
Clone ID Related Accession (Same CDS sequence)	NM_080632.2 , NM_080632.3
Accession Version	NM_080632.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 3B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA949746.1, AF318576.1 and CN412717.1. This sequence is a reference standard in the RefSeqGene project. On Aug 21, 2010 this sequence version replaced NM_080632.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1), also known as hUpf3-X. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.207054.1, SRR1803615.262131.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GAAAGTGGTA TTAGCCATAG AAAAGAAGGA 
GGAGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542199.1
CDS	101..1552
Translation	MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQKNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE

Target ORF information:

RefSeq Version	NM_080632.2
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080632.2

ORF Insert Sequence:

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GAAAGTGGTA TTAGCCATAG AAAAGAAGGA 
GGAGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF3B ( NM_080632.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_542199.1 Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18945
Clone ID Related Accession (Same CDS sequence)	NM_080632.2 , NM_080632.3
Accession Version	NM_080632.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 3B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC121017.1, AF318576.1 and AA657616.1. On Oct 30, 2019 this sequence version replaced NM_080632.2. Transcript Variant: This variant (1) encodes the longer protein (isoform 1), also known as hUpf3-X. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GAAAGTGGTA TTAGCCATAG AAAAGAAGGA 
GGAGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_542199.1
CDS	36..1487
Translation	MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQKNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE

Target ORF information:

RefSeq Version	NM_080632.3
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080632.3

ORF Insert Sequence:

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GAAAGTGGTA TTAGCCATAG AAAAGAAGGA 
GGAGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF3B ( XM_017029737.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885226.1 Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu99427
Clone ID Related Accession (Same CDS sequence)	XM_017029737.1
Accession Version	XM_017029737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GGTCGTCCAG GGATGCAGTC TTACCATCCA 
GGAGTTCAAA GCCACTATCA ACACTATTTT CCTGATGACA GCACCAACTA TAGATACTCA 
CCAGTAGAAG AAAAGCAGGA AAGTAGTATT AGCTGTAGAA AAGAAGGAGG AGGCCAGGCG 
TGGTGGCGCA CACCTGTAAT CCCAGCACTT TGGGAGGCTG AGGCGGGCAG ATCGCATGAG 
GACTGTCCAT GGATGCAGTG TTACCATCGA GGAGCTCGAG GCCGAGGTCG ACTCTGGCAC 
CCTGATGACA AGTCTGGAGA TTCAGCAGTA GAAAGAAAGC AGAATCATCC AGGGATGCAG 
CGTTACCATC CAGGAGCTCG AAGCCAAGGT CGACTCTCGC ACCCTGATGA CATAACCAAG 
TCTGGAGATT CAGCAGTAGA AAGAAAGCAG GAAAGTTGTA TTAGCCATAC AAAAGAAGAG 
GAGAGGCTGG GTGTGATGGT TCACGCCTGT AATCCCAGCA CCTTGGGAGG CCGAGGCGGG 
TGGATTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885226.1
CDS	101..2032
Translation	MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQKNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQGRPGMQSYHPGVQSHYQHYFPDDSTNYRYSPVEEKQESSISCRKEGGGQAWWRTPVIPALWEAEAGRSHEDCPWMQCYHRGARGRGRLWHPDDKSGDSAVERKQNHPGMQRYHPGARSQGRLSHPDDITKSGDSAVERKQESCISHTKEEERLGVMVHACNPSTLGGRGGWIT

Target ORF information:

RefSeq Version	XM_017029737.1
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029737.1

ORF Insert Sequence:

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GGTCGTCCAG GGATGCAGTC TTACCATCCA 
GGAGTTCAAA GCCACTATCA ACACTATTTT CCTGATGACA GCACCAACTA TAGATACTCA 
CCAGTAGAAG AAAAGCAGGA AAGTAGTATT AGCTGTAGAA AAGAAGGAGG AGGCCAGGCG 
TGGTGGCGCA CACCTGTAAT CCCAGCACTT TGGGAGGCTG AGGCGGGCAG ATCGCATGAG 
GACTGTCCAT GGATGCAGTG TTACCATCGA GGAGCTCGAG GCCGAGGTCG ACTCTGGCAC 
CCTGATGACA AGTCTGGAGA TTCAGCAGTA GAAAGAAAGC AGAATCATCC AGGGATGCAG 
CGTTACCATC CAGGAGCTCG AAGCCAAGGT CGACTCTCGC ACCCTGATGA CATAACCAAG 
TCTGGAGATT CAGCAGTAGA AAGAAAGCAG GAAAGTTGTA TTAGCCATAC AAAAGAAGAG 
GAGAGGCTGG GTGTGATGGT TCACGCCTGT AATCCCAGCA CCTTGGGAGG CCGAGGCGGG 
TGGATTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF3B ( XM_017029738.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885227.1 Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu99428
Clone ID Related Accession (Same CDS sequence)	XM_017029738.1
Accession Version	XM_017029738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 3B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GGTCGTCCAG GGATGCAGTC TTACCATCCA 
GGAGTTCAAA GCCACTATCA ACACTATTTT CCTGATGACA GCACCAACTA TAGATACTCA 
CCAGTAGAAG AAAAGCAGGA AAGTAGTATT AGCTGTAGAA AAGAAGGAGG AGGCCAGGCG 
TGGTGGCGCA CACCTGTAAT CCCAGCACTT TGGGAGGCTG AGGCGGGCAG ATCGCATGAG 
GACTGTCCAT GGATGCAGTG TTACCATCGA GGAGCTCGAG GCCGAGGTCG ACTCTGGCAC 
CCTGATGACA AGTCTGGAGA TTCAGCAGTA GAAAGAAAGC AGAATCATCC AGGGATGCAG 
CGTTACCATC CAGGAGCTCG AAGCCAAGGT CGACTCTCGC ACCCTGATGA CATAACCAAG 
TCTGGAGATT CAGCAGTAGA AAGAAAGCAG GATCATCCAG GGATGAAGCT TTACCATCCA 
GGAGCTCGAA GCCCAGGTCG ATTCTGGTGG CAAGATGGCA GCACCAATCT GGAGATTCAG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885227.1
CDS	101..2026
Translation	MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQKNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQGRPGMQSYHPGVQSHYQHYFPDDSTNYRYSPVEEKQESSISCRKEGGGQAWWRTPVIPALWEAEAGRSHEDCPWMQCYHRGARGRGRLWHPDDKSGDSAVERKQNHPGMQRYHPGARSQGRLSHPDDITKSGDSAVERKQDHPGMKLYHPGARSPGRFWWQDGSTNLEIQQ

Target ORF information:

RefSeq Version	XM_017029738.1
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029738.1

ORF Insert Sequence:

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GGTCGTCCAG GGATGCAGTC TTACCATCCA 
GGAGTTCAAA GCCACTATCA ACACTATTTT CCTGATGACA GCACCAACTA TAGATACTCA 
CCAGTAGAAG AAAAGCAGGA AAGTAGTATT AGCTGTAGAA AAGAAGGAGG AGGCCAGGCG 
TGGTGGCGCA CACCTGTAAT CCCAGCACTT TGGGAGGCTG AGGCGGGCAG ATCGCATGAG 
GACTGTCCAT GGATGCAGTG TTACCATCGA GGAGCTCGAG GCCGAGGTCG ACTCTGGCAC 
CCTGATGACA AGTCTGGAGA TTCAGCAGTA GAAAGAAAGC AGAATCATCC AGGGATGCAG 
CGTTACCATC CAGGAGCTCG AAGCCAAGGT CGACTCTCGC ACCCTGATGA CATAACCAAG 
TCTGGAGATT CAGCAGTAGA AAGAAAGCAG GATCATCCAG GGATGAAGCT TTACCATCCA 
GGAGCTCGAA GCCCAGGTCG ATTCTGGTGG CAAGATGGCA GCACCAATCT GGAGATTCAG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF3B ( XM_017029739.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885228.1 Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu99429
Clone ID Related Accession (Same CDS sequence)	XM_017029739.1
Accession Version	XM_017029739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 3B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGCTG CTCAAGAAGC CAGAAAAAGG AGATGAAAAA 
GAATTGGACA AAAGAGAAAA AGCCAAGAAA TTGGACAAAG AGAATCTCAG TGATGAAAGA 
GCCAGTGGGC AAAGTTGTAC ATTGCCCAAG CGTTCTGATA GCGAACTTAA AGATGAAAAA 
CCAAAGAGAC CTGAAGATGA GAGCGGCAGA GACTATAGGG AGAGGGAACG GGAATATGAA 
CGAGATCAGG AGCGCATACT TCGAGAAAGA GAGAGGCTGA AGCGGCAAGA AGAAGAGCGC 
CGTAGGCAGA AGGAGCGCTA TGAGAAAGAG AAGACTTTTA AGAGAAAAGA AGAAGAAATG 
AAAAAAGAGA AAGACACACT TCGGGATAAA GGAAAGAAGG CTGAAAGTAC AGAATCAATA 
GGCAGCTCAG AAAAAACTGA AAAGAAAGAA GAAGTGGTCA AGAGAGATCG AATAAGAAAC 
AAGGATCGTC CAGCGATGCA GCTTTACCAA CCAGGAGCTC GAAGCCGAAA TCGACTCTGT 
CCCCCTGATG ACAGCACCAA GTCTGGAGAT TCAGCAGCAG AAAGGAAGCA GGGTCGTCCA 
GGGATGCAGT CTTACCATCC AGGAGTTCAA AGCCACTATC AACACTATTT TCCTGATGAC 
AGCACCAACT ATAGATACTC ACCAGTAGAA GAAAAGCAGG AAAGTAGTAT TAGCTGTAGA 
AAAGAAGGAG GAGGCCAGGC GTGGTGGCGC ACACCTGTAA TCCCAGCACT TTGGGAGGCT 
GAGGCGGGCA GATCGCATGA GGACTGTCCA TGGATGCAGT GTTACCATCG AGGAGCTCGA 
GGCCGAGGTC GACTCTGGCA CCCTGATGAC AAGTCTGGAG ATTCAGCAGT AGAAAGAAAG 
CAGAATCATC CAGGGATGCA GCGTTACCAT CCAGGAGCTC GAAGCCAAGG TCGACTCTCG 
CACCCTGATG ACATAACCAA GTCTGGAGAT TCAGCAGTAG AAAGAAAGCA GGAAAGTTGT 
ATTAGCCATA CAAAAGAAGA GGAGAGGCTG GGTGTGATGG TTCACGCCTG TAATCCCAGC 
ACCTTGGGAG GCCGAGGCGG GTGGATTACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885228.1
CDS	101..1993
Translation	MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQGRPGMQSYHPGVQSHYQHYFPDDSTNYRYSPVEEKQESSISCRKEGGGQAWWRTPVIPALWEAEAGRSHEDCPWMQCYHRGARGRGRLWHPDDKSGDSAVERKQNHPGMQRYHPGARSQGRLSHPDDITKSGDSAVERKQESCISHTKEEERLGVMVHACNPSTLGGRGGWIT

Target ORF information:

RefSeq Version	XM_017029739.1
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029739.1

ORF Insert Sequence:

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGCTG CTCAAGAAGC CAGAAAAAGG AGATGAAAAA 
GAATTGGACA AAAGAGAAAA AGCCAAGAAA TTGGACAAAG AGAATCTCAG TGATGAAAGA 
GCCAGTGGGC AAAGTTGTAC ATTGCCCAAG CGTTCTGATA GCGAACTTAA AGATGAAAAA 
CCAAAGAGAC CTGAAGATGA GAGCGGCAGA GACTATAGGG AGAGGGAACG GGAATATGAA 
CGAGATCAGG AGCGCATACT TCGAGAAAGA GAGAGGCTGA AGCGGCAAGA AGAAGAGCGC 
CGTAGGCAGA AGGAGCGCTA TGAGAAAGAG AAGACTTTTA AGAGAAAAGA AGAAGAAATG 
AAAAAAGAGA AAGACACACT TCGGGATAAA GGAAAGAAGG CTGAAAGTAC AGAATCAATA 
GGCAGCTCAG AAAAAACTGA AAAGAAAGAA GAAGTGGTCA AGAGAGATCG AATAAGAAAC 
AAGGATCGTC CAGCGATGCA GCTTTACCAA CCAGGAGCTC GAAGCCGAAA TCGACTCTGT 
CCCCCTGATG ACAGCACCAA GTCTGGAGAT TCAGCAGCAG AAAGGAAGCA GGGTCGTCCA 
GGGATGCAGT CTTACCATCC AGGAGTTCAA AGCCACTATC AACACTATTT TCCTGATGAC 
AGCACCAACT ATAGATACTC ACCAGTAGAA GAAAAGCAGG AAAGTAGTAT TAGCTGTAGA 
AAAGAAGGAG GAGGCCAGGC GTGGTGGCGC ACACCTGTAA TCCCAGCACT TTGGGAGGCT 
GAGGCGGGCA GATCGCATGA GGACTGTCCA TGGATGCAGT GTTACCATCG AGGAGCTCGA 
GGCCGAGGTC GACTCTGGCA CCCTGATGAC AAGTCTGGAG ATTCAGCAGT AGAAAGAAAG 
CAGAATCATC CAGGGATGCA GCGTTACCAT CCAGGAGCTC GAAGCCAAGG TCGACTCTCG 
CACCCTGATG ACATAACCAA GTCTGGAGAT TCAGCAGTAG AAAGAAAGCA GGAAAGTTGT 
ATTAGCCATA CAAAAGAAGA GGAGAGGCTG GGTGTGATGG TTCACGCCTG TAATCCCAGC 
ACCTTGGGAG GCCGAGGCGG GTGGATTACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF3B ( XM_017029740.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885229.1 Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X4, mRNA.

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CloneID	OHu99430
Clone ID Related Accession (Same CDS sequence)	XM_017029740.1
Accession Version	XM_017029740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	regulator of nonsense transcripts 3B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GGTCGTCCAG GGATGCAGTC TTACCATCCA 
GGAGTTCAAA GCCACTATCA ACACTATTTT CCTGATGACA GCACCAACTA TAGATACTCA 
CCAGTAGAAG AAAAGCAGGA CTGTCCATGG ATGCAGTGTT ACCATCGAGG AGCTCGAGGC 
CGAGGTCGAC TCTGGCACCC TGATGACAAG TCTGGAGATT CAGCAGTAGA AAGAAAGCAG 
AATCATCCAG GGATGCAGCG TTACCATCCA GGAGCTCGAA GCCAAGGTCG ACTCTCGCAC 
CCTGATGACA TAACCAAGTC TGGAGATTCA GCAGTAGAAA GAAAGCAGGA AAGTTGTATT 
AGCCATACAA AAGAAGAGGA GAGGCTGGGT GTGATGGTTC ACGCCTGTAA TCCCAGCACC 
TTGGGAGGCC GAGGCGGGTG GATTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885229.1
CDS	101..1930
Translation	MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEHLQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAAKKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRMREEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQKNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQGRPGMQSYHPGVQSHYQHYFPDDSTNYRYSPVEEKQDCPWMQCYHRGARGRGRLWHPDDKSGDSAVERKQNHPGMQRYHPGARSQGRLSHPDDITKSGDSAVERKQESCISHTKEEERLGVMVHACNPSTLGGRGGWIT

Target ORF information:

RefSeq Version	XM_017029740.1
Organism	Homo sapiens(human)
Definition	Homo sapiens UPF3B regulator of nonsense mediated mRNA decay (UPF3B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029740.1

ORF Insert Sequence:

ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC 
GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG 
GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC 
ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT 
GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC 
TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT 
GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA 
AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT 
AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA 
CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT 
TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG 
AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG 
AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC 
AAATTAAAGG ATGAACCAAA GATTAAGGTA CACAGGTTTC TGTTACAAGC TGTGAATCAG 
AAAAATCTGC TCAAGAAGCC AGAAAAAGGA GATGAAAAAG AATTGGACAA AAGAGAAAAA 
GCCAAGAAAT TGGACAAAGA GAATCTCAGT GATGAAAGAG CCAGTGGGCA AAGTTGTACA 
TTGCCCAAGC GTTCTGATAG CGAACTTAAA GATGAAAAAC CAAAGAGACC TGAAGATGAG 
AGCGGCAGAG ACTATAGGGA GAGGGAACGG GAATATGAAC GAGATCAGGA GCGCATACTT 
CGAGAAAGAG AGAGGCTGAA GCGGCAAGAA GAAGAGCGCC GTAGGCAGAA GGAGCGCTAT 
GAGAAAGAGA AGACTTTTAA GAGAAAAGAA GAAGAAATGA AAAAAGAGAA AGACACACTT 
CGGGATAAAG GAAAGAAGGC TGAAAGTACA GAATCAATAG GCAGCTCAGA AAAAACTGAA 
AAGAAAGAAG AAGTGGTCAA GAGAGATCGA ATAAGAAACA AGGATCGTCC AGCGATGCAG 
CTTTACCAAC CAGGAGCTCG AAGCCGAAAT CGACTCTGTC CCCCTGATGA CAGCACCAAG 
TCTGGAGATT CAGCAGCAGA AAGGAAGCAG GGTCGTCCAG GGATGCAGTC TTACCATCCA 
GGAGTTCAAA GCCACTATCA ACACTATTTT CCTGATGACA GCACCAACTA TAGATACTCA 
CCAGTAGAAG AAAAGCAGGA CTGTCCATGG ATGCAGTGTT ACCATCGAGG AGCTCGAGGC 
CGAGGTCGAC TCTGGCACCC TGATGACAAG TCTGGAGATT CAGCAGTAGA AAGAAAGCAG 
AATCATCCAG GGATGCAGCG TTACCATCCA GGAGCTCGAA GCCAAGGTCG ACTCTCGCAC 
CCTGATGACA TAACCAAGTC TGGAGATTCA GCAGTAGAAA GAAAGCAGGA AAGTTGTATT 
AGCCATACAA AAGAAGAGGA GAGGCTGGGT GTGATGGTTC ACGCCTGTAA TCCCAGCACC 
TTGGGAGGCC GAGGCGGGTG GATTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dual function of UPF3B in early and late translation termination.
The EMBO journal36(20)2968-2986(2017 10)
Neu-Yilik G,Raimondeau E,Eliseev B,Yeramala L,Amthor B,Deniaud A,Huard K,Kerschgens K,Hentze MW,Schaffitzel C,Kulozik AE

Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex.
Proceedings of the National Academy of Sciences of the United States of America107(22)10050-5(2010 Jun)
Buchwald G,Ebert J,Basquin C,Sauliere J,Jayachandran U,Bono F,Le Hir H,Conti E

A UPF3-mediated regulatory switch that maintains RNA surveillance.
Nature structural & molecular biology16(7)747-53(2009 Jul)
Chan WK,Bhalla AD,Le Hir H,Nguyen LS,Huang L,G?cz J,Wilkinson MF

Nonsense mediated decay induced by tethered human UPF3B is restricted to the cytoplasm.
RNA biology1(1)42-7(2004 May)
Lu S,Cullen BR

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UPF3B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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