EFCAB6 cDNA ORF clone, Homo sapiens(human)

The following EFCAB6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFCAB6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu19563 NM_022785.4
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu53657 XM_011530329.1
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu53657 XM_024452263.1
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu71510 XM_011530328.3
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu71511 XM_011530330.3
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu71508 XM_011530326.3
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu71509 XM_011530327.2
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu93563 XM_017028911.2
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu32342 NM_198856.3
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu53654 XM_005261704.2
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu53654 XM_011530317.3
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu53655 XM_005261705.4
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu53655 XM_011530319.3
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71501 XM_011530316.1
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu71501 XM_017028910.2
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu71502 XM_011530318.3
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu71503 XM_011530320.3
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71504 XM_011530321.3
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71504 XM_011530322.2
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71504 XM_024452262.1
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71505 XM_011530323.2
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71506 XM_017028912.2
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71507 XM_011530325.2
Latest version!
Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu93563 XM_017028911.1 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu71504 XM_011530321.2 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu19563 NM_022785.3 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu32342 NM_198856.2 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71501 XM_017028910.1 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu71502 XM_011530318.2 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu71510 XM_011530328.2 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu71511 XM_011530330.2 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu53654 XM_011530317.2 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu53655 XM_005261705.3 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu53655 XM_011530319.2 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71506 XM_017028912.1 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu71508 XM_011530326.2 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu71503 XM_011530320.2 Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu19563
    Clone ID Related Accession (Same CDS sequence) NM_022785.4 , NM_022785.3
    Accession Version NM_022785.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK058069.1, AK292411.1, BC039315.1 and BP325901.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_022785.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK292411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262726.12/ ENSP00000262726.7 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_073622.2
    CDS283..4788
    Translation

    Target ORF information:

    RefSeq Version NM_022785.4
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022785.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19563
    Clone ID Related Accession (Same CDS sequence) NM_022785.4 , NM_022785.3
    Accession Version NM_022785.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK058069.1, AK292411.1, BC039315.1, BP325901.1 and AA778573.1. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2010 this sequence version replaced NM_022785.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK292411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_073622.2
    CDS315..4820
    Translation

    Target ORF information:

    RefSeq Version NM_022785.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022785.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53657
    Clone ID Related Accession (Same CDS sequence) XM_011530329.1 , XM_024452263.1
    Accession Version XM_011530329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2658bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGAGGGC CGGAAACCAC TCCGCCGCAG CCTCCAACTC CTTCAAAAAG TTACGTGAAC 
    AGCCACTTTA TCACCGCAGA AGAATGCCTG AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA
    TTCCGGGACC CCTACTCTGC CTTCTTTAAA ACAGATGCTG ACAGGGATGG CATAATCAAC
    ATGCATGACC TTCACAGACT GCTCCTGCAT CTACTGCTTA ATCTCAAAGA CGACGAGTTT
    GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA CTTAGTGTCA CTTTAAATTT TCGGGAATTT
    CAAAATTTGT GTGAGAAGAG ACCATGGAGA ACAGATGAAG CGCCTCAAAG ACTCATTAGA
    CCAAAACAGA AGGTTGCGGA TTCAGAACTA GCTTGTGAGC AGGCTCATCA GTATCTTGTT
    ACCAAAGCAA AAAACAGATG GTCAGACTTG TCTAAGAATT TTCTAGAAAC CGATAATGAG
    GGCAATGGCA TTCTTCGACG CCGGGACATA AAGAACGCAC TGTACGGTTT TGATATTCCC
    CTCACACCAA GAGAGTTTGA AAAGCTTTGG GCAAGATACG ACACCGAGGG AAAAGGGCAC
    ATTACTTACC AGGAATTTTT ACAGAAATTG GGTATTAACT ATTCGCCTGC TGTCCATCGG
    CCCTGTGCAG AGGATTATTT CAACTTCATG GGTCATTTTA CAAAGCCACA GCAGCTACAG
    GAAGAGATGA AGGAGCTGCA GCAGAGCACA GAGAAGGCTG TGGCAGCCAG GGATAAGCTT
    ATGGACCGCC ATCAAGATAT CAGCAAAGCA TTCACCAAAA CTGATCAATC CAAAACCAAC
    TACATATCCA TATGCAAGAT GCAGGAAGTG CTGGAAGAAT GTGGATGTTC TCTTACCGAA
    GGGGAGCTGA CCCATCTGCT AAACAGTTGG GGAGTCAGCC GGCATGATAA TGCTATCAAT
    TACCTCGACT TCCTGAGAGC AGTGGAGAAC AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA
    AAAGAAGAGA GCATGCCAAT CAATTTTGCA ACGCTGAATC CACAGGAGGC TGTGAGGAAG
    ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG GCTTTGTCCA CGGCATTTTC TGCATTGGAT
    AAAGAGGATA CAGGATTTGT AAAGGCTACA GAATTCGGAC AAGTTCTTAA GGATTTCTGT
    TACAAACTAA CGGACAATCA GTATCATTAT TTTTTGAGGA AACTAAGAAT TCATCTAACC
    CCCTATATAA ATTGGAAATA TTTTCTCCAG AACTTTAGCT GTTTCCTTGA GGAGACAGCT
    GATGAGTGGG CTGAGAAAAT GCCCAAAGGC CCGCCGCCTA CCTCTCCCAA GGCCACAGCC
    GACAGAGACA TCCTGGCTCG CCTCCACAAA GCAGTGACTT CCCATTACCA TGCCATCACC
    CAGGAGTTTG AGAATTTTGA CACCATGAAA ACGAACACCA TCTCCAGAGA GGAGTTTAGG
    GCCATTTGTA ATCGCCGCGT CCAAATCCTG ACGGACGAAC AGTTTGACAG ACTCTGGAAC
    GAGATGCCAG TCAATGCCAA GGGGAGGCTG AAATACCCGG ACTTCCTGAG CAGGTTCAGT
    TCCGAGACAG CAGCCACACC AATGGCCACT GGTGACTCGG CCGTGGCCCA GAGAGGGAGC
    AGTGTCCCTG ACGTCTCGGA AGGCACCAGA TCTGCCCTCT CATTGCCCAC TCAGGAGCTG
    AGACCAGGGT CAAAGTCGCA GAGCCACCCC TGTACTCCAG CGAGCACCAC CGTGATCCCG
    GGCACTCCAC CCTTGCAGAA CTGTGATCCC ATAGAGAGCA GGCTCCGGAA GAGAATCCAG
    GGCTGCTGGC GCCAGCTCCT GAAAGAATGC AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC
    ATCAACGCCT CCGATTTCCT GGCTCTTGTG GAGAAATTCA ACCTGGACAT AAGCAAAGAG
    GAGTGTCAGC AGCTCATTAT AAAATACGAC TTAAAGAGCA ACGGGAAATT TGCATACTGT
    GACTTCATTC AGAGCTGTGT CCTCCTGCTA AAAGCAAAGG AAAGCTCACT GATGCACAGG
    ATGAAGATCC AGAATGCACA CAAGATGAAA GAAGCCGGCG CGGAGACGCC ATCTTTTTAC
    TCTGCTCTGC TGCGTATTCA GCCCAAAATT GTGCACTGCT GGAGGCCAAT GCGGCGCACG
    TTCAAAAGCT ATGATGAGGC TGGAACAGGG CTGCTAAGCG TCGCAGATTT CAGGACGGTG
    AGCACAGCTC TGCGCCCAGT GCCTCTGCAG GGAGCAGCTG CCAAATGCCA GCGGCCAGAA
    ACTTCCCACG GGGAGCCCTC TACCTGCTGC CGAAGGCCCG GTGTGACCTG GCTGCTTCAG
    ATCTCACTTC CCTGGGACCC CCAAGTTATC CAGTCCTGGG TCCACTCGCC TCACCTTCAG
    ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG GAGCCCTGCG TGGATGCCCC ATACCTGCAT
    CGCCAGCTCC TCAGGGTCCT GAGACAGTAC AGCATCAACC TCTCTGAGGA AGAGTTCTTC
    CATATTCTGG AGTATTACGA TAAGACGCTG TCTTCAAAAA TCTCCTACAA CGACTTCCTC
    CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528631.1
    CDS741..3398
    Translation

    Target ORF information:

    RefSeq Version XM_011530329.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGAGGGC CGGAAACCAC TCCGCCGCAG CCTCCAACTC CTTCAAAAAG TTACGTGAAC 
    AGCCACTTTA TCACCGCAGA AGAATGCCTG AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA
    TTCCGGGACC CCTACTCTGC CTTCTTTAAA ACAGATGCTG ACAGGGATGG CATAATCAAC
    ATGCATGACC TTCACAGACT GCTCCTGCAT CTACTGCTTA ATCTCAAAGA CGACGAGTTT
    GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA CTTAGTGTCA CTTTAAATTT TCGGGAATTT
    CAAAATTTGT GTGAGAAGAG ACCATGGAGA ACAGATGAAG CGCCTCAAAG ACTCATTAGA
    CCAAAACAGA AGGTTGCGGA TTCAGAACTA GCTTGTGAGC AGGCTCATCA GTATCTTGTT
    ACCAAAGCAA AAAACAGATG GTCAGACTTG TCTAAGAATT TTCTAGAAAC CGATAATGAG
    GGCAATGGCA TTCTTCGACG CCGGGACATA AAGAACGCAC TGTACGGTTT TGATATTCCC
    CTCACACCAA GAGAGTTTGA AAAGCTTTGG GCAAGATACG ACACCGAGGG AAAAGGGCAC
    ATTACTTACC AGGAATTTTT ACAGAAATTG GGTATTAACT ATTCGCCTGC TGTCCATCGG
    CCCTGTGCAG AGGATTATTT CAACTTCATG GGTCATTTTA CAAAGCCACA GCAGCTACAG
    GAAGAGATGA AGGAGCTGCA GCAGAGCACA GAGAAGGCTG TGGCAGCCAG GGATAAGCTT
    ATGGACCGCC ATCAAGATAT CAGCAAAGCA TTCACCAAAA CTGATCAATC CAAAACCAAC
    TACATATCCA TATGCAAGAT GCAGGAAGTG CTGGAAGAAT GTGGATGTTC TCTTACCGAA
    GGGGAGCTGA CCCATCTGCT AAACAGTTGG GGAGTCAGCC GGCATGATAA TGCTATCAAT
    TACCTCGACT TCCTGAGAGC AGTGGAGAAC AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA
    AAAGAAGAGA GCATGCCAAT CAATTTTGCA ACGCTGAATC CACAGGAGGC TGTGAGGAAG
    ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG GCTTTGTCCA CGGCATTTTC TGCATTGGAT
    AAAGAGGATA CAGGATTTGT AAAGGCTACA GAATTCGGAC AAGTTCTTAA GGATTTCTGT
    TACAAACTAA CGGACAATCA GTATCATTAT TTTTTGAGGA AACTAAGAAT TCATCTAACC
    CCCTATATAA ATTGGAAATA TTTTCTCCAG AACTTTAGCT GTTTCCTTGA GGAGACAGCT
    GATGAGTGGG CTGAGAAAAT GCCCAAAGGC CCGCCGCCTA CCTCTCCCAA GGCCACAGCC
    GACAGAGACA TCCTGGCTCG CCTCCACAAA GCAGTGACTT CCCATTACCA TGCCATCACC
    CAGGAGTTTG AGAATTTTGA CACCATGAAA ACGAACACCA TCTCCAGAGA GGAGTTTAGG
    GCCATTTGTA ATCGCCGCGT CCAAATCCTG ACGGACGAAC AGTTTGACAG ACTCTGGAAC
    GAGATGCCAG TCAATGCCAA GGGGAGGCTG AAATACCCGG ACTTCCTGAG CAGGTTCAGT
    TCCGAGACAG CAGCCACACC AATGGCCACT GGTGACTCGG CCGTGGCCCA GAGAGGGAGC
    AGTGTCCCTG ACGTCTCGGA AGGCACCAGA TCTGCCCTCT CATTGCCCAC TCAGGAGCTG
    AGACCAGGGT CAAAGTCGCA GAGCCACCCC TGTACTCCAG CGAGCACCAC CGTGATCCCG
    GGCACTCCAC CCTTGCAGAA CTGTGATCCC ATAGAGAGCA GGCTCCGGAA GAGAATCCAG
    GGCTGCTGGC GCCAGCTCCT GAAAGAATGC AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC
    ATCAACGCCT CCGATTTCCT GGCTCTTGTG GAGAAATTCA ACCTGGACAT AAGCAAAGAG
    GAGTGTCAGC AGCTCATTAT AAAATACGAC TTAAAGAGCA ACGGGAAATT TGCATACTGT
    GACTTCATTC AGAGCTGTGT CCTCCTGCTA AAAGCAAAGG AAAGCTCACT GATGCACAGG
    ATGAAGATCC AGAATGCACA CAAGATGAAA GAAGCCGGCG CGGAGACGCC ATCTTTTTAC
    TCTGCTCTGC TGCGTATTCA GCCCAAAATT GTGCACTGCT GGAGGCCAAT GCGGCGCACG
    TTCAAAAGCT ATGATGAGGC TGGAACAGGG CTGCTAAGCG TCGCAGATTT CAGGACGGTG
    AGCACAGCTC TGCGCCCAGT GCCTCTGCAG GGAGCAGCTG CCAAATGCCA GCGGCCAGAA
    ACTTCCCACG GGGAGCCCTC TACCTGCTGC CGAAGGCCCG GTGTGACCTG GCTGCTTCAG
    ATCTCACTTC CCTGGGACCC CCAAGTTATC CAGTCCTGGG TCCACTCGCC TCACCTTCAG
    ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG GAGCCCTGCG TGGATGCCCC ATACCTGCAT
    CGCCAGCTCC TCAGGGTCCT GAGACAGTAC AGCATCAACC TCTCTGAGGA AGAGTTCTTC
    CATATTCTGG AGTATTACGA TAAGACGCTG TCTTCAAAAA TCTCCTACAA CGACTTCCTC
    CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53657
    Clone ID Related Accession (Same CDS sequence) XM_011530329.1 , XM_024452263.1
    Accession Version XM_024452263.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2658bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGAGGGC CGGAAACCAC TCCGCCGCAG CCTCCAACTC CTTCAAAAAG TTACGTGAAC 
    AGCCACTTTA TCACCGCAGA AGAATGCCTG AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA
    TTCCGGGACC CCTACTCTGC CTTCTTTAAA ACAGATGCTG ACAGGGATGG CATAATCAAC
    ATGCATGACC TTCACAGACT GCTCCTGCAT CTACTGCTTA ATCTCAAAGA CGACGAGTTT
    GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA CTTAGTGTCA CTTTAAATTT TCGGGAATTT
    CAAAATTTGT GTGAGAAGAG ACCATGGAGA ACAGATGAAG CGCCTCAAAG ACTCATTAGA
    CCAAAACAGA AGGTTGCGGA TTCAGAACTA GCTTGTGAGC AGGCTCATCA GTATCTTGTT
    ACCAAAGCAA AAAACAGATG GTCAGACTTG TCTAAGAATT TTCTAGAAAC CGATAATGAG
    GGCAATGGCA TTCTTCGACG CCGGGACATA AAGAACGCAC TGTACGGTTT TGATATTCCC
    CTCACACCAA GAGAGTTTGA AAAGCTTTGG GCAAGATACG ACACCGAGGG AAAAGGGCAC
    ATTACTTACC AGGAATTTTT ACAGAAATTG GGTATTAACT ATTCGCCTGC TGTCCATCGG
    CCCTGTGCAG AGGATTATTT CAACTTCATG GGTCATTTTA CAAAGCCACA GCAGCTACAG
    GAAGAGATGA AGGAGCTGCA GCAGAGCACA GAGAAGGCTG TGGCAGCCAG GGATAAGCTT
    ATGGACCGCC ATCAAGATAT CAGCAAAGCA TTCACCAAAA CTGATCAATC CAAAACCAAC
    TACATATCCA TATGCAAGAT GCAGGAAGTG CTGGAAGAAT GTGGATGTTC TCTTACCGAA
    GGGGAGCTGA CCCATCTGCT AAACAGTTGG GGAGTCAGCC GGCATGATAA TGCTATCAAT
    TACCTCGACT TCCTGAGAGC AGTGGAGAAC AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA
    AAAGAAGAGA GCATGCCAAT CAATTTTGCA ACGCTGAATC CACAGGAGGC TGTGAGGAAG
    ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG GCTTTGTCCA CGGCATTTTC TGCATTGGAT
    AAAGAGGATA CAGGATTTGT AAAGGCTACA GAATTCGGAC AAGTTCTTAA GGATTTCTGT
    TACAAACTAA CGGACAATCA GTATCATTAT TTTTTGAGGA AACTAAGAAT TCATCTAACC
    CCCTATATAA ATTGGAAATA TTTTCTCCAG AACTTTAGCT GTTTCCTTGA GGAGACAGCT
    GATGAGTGGG CTGAGAAAAT GCCCAAAGGC CCGCCGCCTA CCTCTCCCAA GGCCACAGCC
    GACAGAGACA TCCTGGCTCG CCTCCACAAA GCAGTGACTT CCCATTACCA TGCCATCACC
    CAGGAGTTTG AGAATTTTGA CACCATGAAA ACGAACACCA TCTCCAGAGA GGAGTTTAGG
    GCCATTTGTA ATCGCCGCGT CCAAATCCTG ACGGACGAAC AGTTTGACAG ACTCTGGAAC
    GAGATGCCAG TCAATGCCAA GGGGAGGCTG AAATACCCGG ACTTCCTGAG CAGGTTCAGT
    TCCGAGACAG CAGCCACACC AATGGCCACT GGTGACTCGG CCGTGGCCCA GAGAGGGAGC
    AGTGTCCCTG ACGTCTCGGA AGGCACCAGA TCTGCCCTCT CATTGCCCAC TCAGGAGCTG
    AGACCAGGGT CAAAGTCGCA GAGCCACCCC TGTACTCCAG CGAGCACCAC CGTGATCCCG
    GGCACTCCAC CCTTGCAGAA CTGTGATCCC ATAGAGAGCA GGCTCCGGAA GAGAATCCAG
    GGCTGCTGGC GCCAGCTCCT GAAAGAATGC AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC
    ATCAACGCCT CCGATTTCCT GGCTCTTGTG GAGAAATTCA ACCTGGACAT AAGCAAAGAG
    GAGTGTCAGC AGCTCATTAT AAAATACGAC TTAAAGAGCA ACGGGAAATT TGCATACTGT
    GACTTCATTC AGAGCTGTGT CCTCCTGCTA AAAGCAAAGG AAAGCTCACT GATGCACAGG
    ATGAAGATCC AGAATGCACA CAAGATGAAA GAAGCCGGCG CGGAGACGCC ATCTTTTTAC
    TCTGCTCTGC TGCGTATTCA GCCCAAAATT GTGCACTGCT GGAGGCCAAT GCGGCGCACG
    TTCAAAAGCT ATGATGAGGC TGGAACAGGG CTGCTAAGCG TCGCAGATTT CAGGACGGTG
    AGCACAGCTC TGCGCCCAGT GCCTCTGCAG GGAGCAGCTG CCAAATGCCA GCGGCCAGAA
    ACTTCCCACG GGGAGCCCTC TACCTGCTGC CGAAGGCCCG GTGTGACCTG GCTGCTTCAG
    ATCTCACTTC CCTGGGACCC CCAAGTTATC CAGTCCTGGG TCCACTCGCC TCACCTTCAG
    ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG GAGCCCTGCG TGGATGCCCC ATACCTGCAT
    CGCCAGCTCC TCAGGGTCCT GAGACAGTAC AGCATCAACC TCTCTGAGGA AGAGTTCTTC
    CATATTCTGG AGTATTACGA TAAGACGCTG TCTTCAAAAA TCTCCTACAA CGACTTCCTC
    CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308031.1
    CDS1195..3852
    Translation

    Target ORF information:

    RefSeq Version XM_024452263.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452263.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGAGGGC CGGAAACCAC TCCGCCGCAG CCTCCAACTC CTTCAAAAAG TTACGTGAAC 
    AGCCACTTTA TCACCGCAGA AGAATGCCTG AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA
    TTCCGGGACC CCTACTCTGC CTTCTTTAAA ACAGATGCTG ACAGGGATGG CATAATCAAC
    ATGCATGACC TTCACAGACT GCTCCTGCAT CTACTGCTTA ATCTCAAAGA CGACGAGTTT
    GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA CTTAGTGTCA CTTTAAATTT TCGGGAATTT
    CAAAATTTGT GTGAGAAGAG ACCATGGAGA ACAGATGAAG CGCCTCAAAG ACTCATTAGA
    CCAAAACAGA AGGTTGCGGA TTCAGAACTA GCTTGTGAGC AGGCTCATCA GTATCTTGTT
    ACCAAAGCAA AAAACAGATG GTCAGACTTG TCTAAGAATT TTCTAGAAAC CGATAATGAG
    GGCAATGGCA TTCTTCGACG CCGGGACATA AAGAACGCAC TGTACGGTTT TGATATTCCC
    CTCACACCAA GAGAGTTTGA AAAGCTTTGG GCAAGATACG ACACCGAGGG AAAAGGGCAC
    ATTACTTACC AGGAATTTTT ACAGAAATTG GGTATTAACT ATTCGCCTGC TGTCCATCGG
    CCCTGTGCAG AGGATTATTT CAACTTCATG GGTCATTTTA CAAAGCCACA GCAGCTACAG
    GAAGAGATGA AGGAGCTGCA GCAGAGCACA GAGAAGGCTG TGGCAGCCAG GGATAAGCTT
    ATGGACCGCC ATCAAGATAT CAGCAAAGCA TTCACCAAAA CTGATCAATC CAAAACCAAC
    TACATATCCA TATGCAAGAT GCAGGAAGTG CTGGAAGAAT GTGGATGTTC TCTTACCGAA
    GGGGAGCTGA CCCATCTGCT AAACAGTTGG GGAGTCAGCC GGCATGATAA TGCTATCAAT
    TACCTCGACT TCCTGAGAGC AGTGGAGAAC AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA
    AAAGAAGAGA GCATGCCAAT CAATTTTGCA ACGCTGAATC CACAGGAGGC TGTGAGGAAG
    ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG GCTTTGTCCA CGGCATTTTC TGCATTGGAT
    AAAGAGGATA CAGGATTTGT AAAGGCTACA GAATTCGGAC AAGTTCTTAA GGATTTCTGT
    TACAAACTAA CGGACAATCA GTATCATTAT TTTTTGAGGA AACTAAGAAT TCATCTAACC
    CCCTATATAA ATTGGAAATA TTTTCTCCAG AACTTTAGCT GTTTCCTTGA GGAGACAGCT
    GATGAGTGGG CTGAGAAAAT GCCCAAAGGC CCGCCGCCTA CCTCTCCCAA GGCCACAGCC
    GACAGAGACA TCCTGGCTCG CCTCCACAAA GCAGTGACTT CCCATTACCA TGCCATCACC
    CAGGAGTTTG AGAATTTTGA CACCATGAAA ACGAACACCA TCTCCAGAGA GGAGTTTAGG
    GCCATTTGTA ATCGCCGCGT CCAAATCCTG ACGGACGAAC AGTTTGACAG ACTCTGGAAC
    GAGATGCCAG TCAATGCCAA GGGGAGGCTG AAATACCCGG ACTTCCTGAG CAGGTTCAGT
    TCCGAGACAG CAGCCACACC AATGGCCACT GGTGACTCGG CCGTGGCCCA GAGAGGGAGC
    AGTGTCCCTG ACGTCTCGGA AGGCACCAGA TCTGCCCTCT CATTGCCCAC TCAGGAGCTG
    AGACCAGGGT CAAAGTCGCA GAGCCACCCC TGTACTCCAG CGAGCACCAC CGTGATCCCG
    GGCACTCCAC CCTTGCAGAA CTGTGATCCC ATAGAGAGCA GGCTCCGGAA GAGAATCCAG
    GGCTGCTGGC GCCAGCTCCT GAAAGAATGC AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC
    ATCAACGCCT CCGATTTCCT GGCTCTTGTG GAGAAATTCA ACCTGGACAT AAGCAAAGAG
    GAGTGTCAGC AGCTCATTAT AAAATACGAC TTAAAGAGCA ACGGGAAATT TGCATACTGT
    GACTTCATTC AGAGCTGTGT CCTCCTGCTA AAAGCAAAGG AAAGCTCACT GATGCACAGG
    ATGAAGATCC AGAATGCACA CAAGATGAAA GAAGCCGGCG CGGAGACGCC ATCTTTTTAC
    TCTGCTCTGC TGCGTATTCA GCCCAAAATT GTGCACTGCT GGAGGCCAAT GCGGCGCACG
    TTCAAAAGCT ATGATGAGGC TGGAACAGGG CTGCTAAGCG TCGCAGATTT CAGGACGGTG
    AGCACAGCTC TGCGCCCAGT GCCTCTGCAG GGAGCAGCTG CCAAATGCCA GCGGCCAGAA
    ACTTCCCACG GGGAGCCCTC TACCTGCTGC CGAAGGCCCG GTGTGACCTG GCTGCTTCAG
    ATCTCACTTC CCTGGGACCC CCAAGTTATC CAGTCCTGGG TCCACTCGCC TCACCTTCAG
    ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG GAGCCCTGCG TGGATGCCCC ATACCTGCAT
    CGCCAGCTCC TCAGGGTCCT GAGACAGTAC AGCATCAACC TCTCTGAGGA AGAGTTCTTC
    CATATTCTGG AGTATTACGA TAAGACGCTG TCTTCAAAAA TCTCCTACAA CGACTTCCTC
    CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71510
    Clone ID Related Accession (Same CDS sequence) XM_011530328.2 , XM_011530328.3
    Accession Version XM_011530328.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGACTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528630.1
    CDS276..3236
    Translation

    Target ORF information:

    RefSeq Version XM_011530328.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGACTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71510
    Clone ID Related Accession (Same CDS sequence) XM_011530328.2 , XM_011530328.3
    Accession Version XM_011530328.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530328.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGACTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528630.1
    CDS984..3944
    Translation

    Target ORF information:

    RefSeq Version XM_011530328.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530328.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGACTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71511
    Clone ID Related Accession (Same CDS sequence) XM_011530330.2 , XM_011530330.3
    Accession Version XM_011530330.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGAATTTTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528632.1
    CDS276..2828
    Translation

    Target ORF information:

    RefSeq Version XM_011530330.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530330.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGAATTTTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71511
    Clone ID Related Accession (Same CDS sequence) XM_011530330.2 , XM_011530330.3
    Accession Version XM_011530330.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530330.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGAATTTTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528632.1
    CDS984..3536
    Translation

    Target ORF information:

    RefSeq Version XM_011530330.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530330.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGAATTTTC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71508
    Clone ID Related Accession (Same CDS sequence) XM_011530326.3 , XM_011530326.2
    Accession Version XM_011530326.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGAGTC TTCCTGAGGC CTCCGCCGAG CGAAGGCCAA ATGGGCGTTT TATTTTTTTA
    ATTTTCTTCT TCACCAGTCA CGTGAGTGCT CACCTCCTGC GAGGCCGGCC TGTCCCACTT
    CTCGCTGGCA CAGGTGCGAC GCTGCCTGTG TTTGCACTGA CACCTGGGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528628.1
    CDS984..4877
    Translation

    Target ORF information:

    RefSeq Version XM_011530326.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530326.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGAGTC TTCCTGAGGC CTCCGCCGAG CGAAGGCCAA ATGGGCGTTT TATTTTTTTA
    ATTTTCTTCT TCACCAGTCA CGTGAGTGCT CACCTCCTGC GAGGCCGGCC TGTCCCACTT
    CTCGCTGGCA CAGGTGCGAC GCTGCCTGTG TTTGCACTGA CACCTGGGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71508
    Clone ID Related Accession (Same CDS sequence) XM_011530326.3 , XM_011530326.2
    Accession Version XM_011530326.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGAGTC TTCCTGAGGC CTCCGCCGAG CGAAGGCCAA ATGGGCGTTT TATTTTTTTA
    ATTTTCTTCT TCACCAGTCA CGTGAGTGCT CACCTCCTGC GAGGCCGGCC TGTCCCACTT
    CTCGCTGGCA CAGGTGCGAC GCTGCCTGTG TTTGCACTGA CACCTGGGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528628.1
    CDS276..4169
    Translation

    Target ORF information:

    RefSeq Version XM_011530326.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530326.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGAGTC TTCCTGAGGC CTCCGCCGAG CGAAGGCCAA ATGGGCGTTT TATTTTTTTA
    ATTTTCTTCT TCACCAGTCA CGTGAGTGCT CACCTCCTGC GAGGCCGGCC TGTCCCACTT
    CTCGCTGGCA CAGGTGCGAC GCTGCCTGTG TTTGCACTGA CACCTGGGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71509
    Clone ID Related Accession (Same CDS sequence) XM_011530327.2
    Accession Version XM_011530327.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAATGTG ATTCGTGCTC TGGAAAGAAT CAGCAGGGGA CTACAATGGA GAATAACAAA 
    GAGGAACTAC TTCTGATTCA GTTTTCTGGA AAGGCTTCTA TGAAAAGATT TGGACTTAAA
    GCCACCACTA AAATCAATTG GAAGCAATTT CTAACATCAT TTCATGAGCC TCAGGGGTTG
    CAAGTTAGCA GTAAAGGTCC CCTGACAAAA AGAAATAGCA TCAACTCTAG AAATGAATCT
    CACAAGGAAA ACATCATCAC AAAGTTATTT AGACACACTG AAGATCACTC TGCGTCACTG
    AAGAAAGCAT TACTGATAAT CAACACTAAA CCCGATGGAC CGATAACAAG AGAAGAATTT
    CGATATATTC TAAATTGCAT GGCTGTAAAA CTAAGCGATT CAGAATTCAA AGAACTAATG
    CAAATGCTTG ACCCTGGGGA CACTGGAGTG GTCAACACCA GCATGTTTAT TGATCTGATT
    GAAGAGAACT GTAGGATGAG AAAGACATCT CCCTGTACAG ATGCTAAGAC ACCTTTCCTC
    CTGGCCTGGG ATTCAGTGGA AGAAATTGTT CATGATACAA TTACTAGGAA CCTACAAGCT
    TTTTATAACA TGCTACGCTC ATATGACCTT GGAGACACAG GGCGCATTGG CCGAAATAAT
    TTCAAGAAAA TCATGCACGT CTTCTGTCCA TTTTTAACGA ATGCACATTT CATAAAACTC
    TGCAGTAAGA TTCAGGACAT TGGTTCAGGA AGAATCCTTT ACAAGAAACT TTTGGCATGC
    ATAGGAATTG ATGGCCCACC CACTGTCTCT CCAGTTCTTG TTCCAAAGGA TCAGCTGTTA
    AGTGAACATT TACAAAAAGA TGAACAGCAG CAGCCAGATC TTTCTGAGAG AACCAAGCTC
    ACGGAGGATA AAACCACCCT GACCAAGAAG ATGACCACAG AAGAAGTGAT TGAAAAATTC
    AAAAAGTGTA TACAGCAGCA GGACCCGGCA TTCAAAAAAC GATTTCTTGA CTTCAGCAAG
    GAGCCTAATG GAAAAATTAA CGTGCATGAC TTTAAGAAGG TACTGGAAGA CACTGGGATG
    CCCATGGACG ATGATCAGTA TGCCCTGCTG ACCACTAAAA TAGGCTTCGA GAAGGAAGGG
    ATGAGCTATC TTGATTTTGC AGCAGGATTT GAAGATCCTC CAATGAGAGG GCCGGAAACC
    ACTCCGCCGC AGCCTCCAAC TCCTTCAAAA AGTTACGTGA ACAGCCACTT TATCACCGCA
    GAAGAATGCC TGAAGCTTTT CCCTAGGAGG CTGAAGGAGT CATTCCGGGA CCCCTACTCT
    GCCTTCTTTA AAACAGATGC TGACAGGGAT GGCATAATCA ACATGCATGA CCTTCACAGA
    CTGCTCCTGC ATCTACTGCT TAATCTCAAA GACGACGAGT TTGAGCGCTT CCTTGGCCTT
    CTTGGCTTGA GACTTAGTGT CACTTTAAAT TTTCGGGAAT TTCAAAATTT GTGTGAGAAG
    AGACCATGGA GAACAGATGA AGCGCCTCAA AGACTCATTA GACCAAAACA GAAGGTTGCG
    GATTCAGAAC TAGCTTGTGA GCAGGCTCAT CAGTATCTTG TTACCAAAGC AAAAAACAGA
    TGGTCAGACT TGTCTAAGAA TTTTCTAGAA ACCGATAATG AGGGCAATGG CATTCTTCGA
    CGCCGGGACA TAAAGAACGC ACTGTACGGT TTTGATATTC CCCTCACACC AAGAGAGTTT
    GAAAAGCTTT GGGCAAGATA CGACACCGAG GGAAAAGGGC ACATTACTTA CCAGGAATTT
    TTACAGAAAT TGGGTATTAA CTATTCGCCT GCTGTCCATC GGCCCTGTGC AGAGGATTAT
    TTCAACTTCA TGGGTCATTT TACAAAGCCA CAGCAGCTAC AGGAAGAGAT GAAGGAGCTG
    CAGCAGAGCA CAGAGAAGGC TGTGGCAGCC AGGGATAAGC TTATGGACCG CCATCAAGAT
    ATCAGCAAAG CATTCACCAA AACTGATCAA TCCAAAACCA ACTACATATC CATATGCAAG
    ATGCAGGAAG TGCTGGAAGA ATGTGGATGT TCTCTTACCG AAGGGGAGCT GACCCATCTG
    CTAAACAGTT GGGGAGTCAG CCGGCATGAT AATGCTATCA ATTACCTCGA CTTCCTGAGA
    GCAGTGGAGA ACAGCAAGTC AACAGGAGCT CAGCCCAAGG AAAAAGAAGA GAGCATGCCA
    ATCAATTTTG CAACGCTGAA TCCACAGGAG GCTGTGAGGA AGATCCAGGA AGTAGTTGAG
    TCCTCCCAGC TGGCTTTGTC CACGGCATTT TCTGCATTGG ATAAAGAGGA TACAGGATTT
    GTAAAGGCTA CAGAATTCGG ACAAGTTCTT AAGGATTTCT GTTACAAACT AACGGACAAT
    CAGTATCATT ATTTTTTGAG GAAACTAAGA ATTCATCTAA CCCCCTATAT AAATTGGAAA
    TATTTTCTCC AGAACTTTAG CTGTTTCCTT GAGGAGACAG CTGATGAGTG GGCTGAGAAA
    ATGCCCAAAG GCCCGCCGCC TACCTCTCCC AAGGCCACAG CCGACAGAGA CATCCTGGCT
    CGCCTCCACA AAGCAGTGAC TTCCCATTAC CATGCCATCA CCCAGGAGTT TGAGAATTTT
    GACACCATGA AAACGAACAC CATCTCCAGA GAGGAGTTTA GGGCCATTTG TAATCGCCGC
    GTCCAAATCC TGACGGACGA ACAGTTTGAC AGACTCTGGA ACGAGATGCC AGTCAATGCC
    AAGGGGAGGC TGAAATACCC GGACTTCCTG AGCAGGTTCA GTTCCGAGAC AGCAGCCACA
    CCAATGGCCA CTGGTGACTC GGCCGTGGCC CAGAGAGGGA GCAGTGTCCC TGACGTCTCG
    GAAGGCACCA GATCTGCCCT CTCATTGCCC ACTCAGGAGC TGAGACCAGG GTCAAAGTCG
    CAGAGCCACC CCTGTACTCC AGCGAGCACC ACCGTGATCC CGGGCACTCC ACCCTTGCAG
    AACTGTGATC CCATAGAGAG CAGGCTCCGG AAGAGAATCC AGGGCTGCTG GCGCCAGCTC
    CTGAAAGAAT GCAAGGAGAA GGACGTGGCC AGACAGGGGG ACATCAACGC CTCCGATTTC
    CTGGCTCTTG TGGAGAAATT CAACCTGGAC ATAAGCAAAG AGGAGTGTCA GCAGCTCATT
    ATAAAATACG ACTTAAAGAG CAACGGGAAA TTTGCATACT GTGACTTCAT TCAGAGCTGT
    GTCCTCCTGC TAAAAGCAAA GGAAAGCTCA CTGATGCACA GGATGAAGAT CCAGAATGCA
    CACAAGATGA AAGAAGCCGG CGCGGAGACG CCATCTTTTT ACTCTGCTCT GCTGCGTATT
    CAGCCCAAAA TTGTGCACTG CTGGAGGCCA ATGCGGCGCA CGTTCAAAAG CTATGATGAG
    GCTGGAACAG GGCTGCTAAG CGTCGCAGAT TTCAGGACGG TGAGCACAGC TCTGCGCCCA
    GTGCCTCTGC AGGGAGCAGC TGCCAAATGC CAGCGGCCAG AAACTTCCCA CGGGGAGCCC
    TCTACCTGCT GCCGAAGGCC CGGTGTGACC TGGCTGCTTC AGATCTCACT TCCCTGGGAC
    CCCCAAGTTA TCCAGTCCTG GGTCCACTCG CCTCACCTTC AGACCCCGGG TTCTCCTTCC
    CAAAGGTTGC TGGAGCCCTG CGTGGATGCC CCATACCTGC ATCGCCAGCT CCTCAGGGTC
    CTGAGACAGT ACAGCATCAA CCTCTCTGAG GAAGAGTTCT TCCATATTCT GGAGTATTAC
    GATAAGACGC TGTCTTCAAA AATCTCCTAC AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528629.1
    CDS87..3926
    Translation

    Target ORF information:

    RefSeq Version XM_011530327.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530327.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCAATGTG ATTCGTGCTC TGGAAAGAAT CAGCAGGGGA CTACAATGGA GAATAACAAA 
    GAGGAACTAC TTCTGATTCA GTTTTCTGGA AAGGCTTCTA TGAAAAGATT TGGACTTAAA
    GCCACCACTA AAATCAATTG GAAGCAATTT CTAACATCAT TTCATGAGCC TCAGGGGTTG
    CAAGTTAGCA GTAAAGGTCC CCTGACAAAA AGAAATAGCA TCAACTCTAG AAATGAATCT
    CACAAGGAAA ACATCATCAC AAAGTTATTT AGACACACTG AAGATCACTC TGCGTCACTG
    AAGAAAGCAT TACTGATAAT CAACACTAAA CCCGATGGAC CGATAACAAG AGAAGAATTT
    CGATATATTC TAAATTGCAT GGCTGTAAAA CTAAGCGATT CAGAATTCAA AGAACTAATG
    CAAATGCTTG ACCCTGGGGA CACTGGAGTG GTCAACACCA GCATGTTTAT TGATCTGATT
    GAAGAGAACT GTAGGATGAG AAAGACATCT CCCTGTACAG ATGCTAAGAC ACCTTTCCTC
    CTGGCCTGGG ATTCAGTGGA AGAAATTGTT CATGATACAA TTACTAGGAA CCTACAAGCT
    TTTTATAACA TGCTACGCTC ATATGACCTT GGAGACACAG GGCGCATTGG CCGAAATAAT
    TTCAAGAAAA TCATGCACGT CTTCTGTCCA TTTTTAACGA ATGCACATTT CATAAAACTC
    TGCAGTAAGA TTCAGGACAT TGGTTCAGGA AGAATCCTTT ACAAGAAACT TTTGGCATGC
    ATAGGAATTG ATGGCCCACC CACTGTCTCT CCAGTTCTTG TTCCAAAGGA TCAGCTGTTA
    AGTGAACATT TACAAAAAGA TGAACAGCAG CAGCCAGATC TTTCTGAGAG AACCAAGCTC
    ACGGAGGATA AAACCACCCT GACCAAGAAG ATGACCACAG AAGAAGTGAT TGAAAAATTC
    AAAAAGTGTA TACAGCAGCA GGACCCGGCA TTCAAAAAAC GATTTCTTGA CTTCAGCAAG
    GAGCCTAATG GAAAAATTAA CGTGCATGAC TTTAAGAAGG TACTGGAAGA CACTGGGATG
    CCCATGGACG ATGATCAGTA TGCCCTGCTG ACCACTAAAA TAGGCTTCGA GAAGGAAGGG
    ATGAGCTATC TTGATTTTGC AGCAGGATTT GAAGATCCTC CAATGAGAGG GCCGGAAACC
    ACTCCGCCGC AGCCTCCAAC TCCTTCAAAA AGTTACGTGA ACAGCCACTT TATCACCGCA
    GAAGAATGCC TGAAGCTTTT CCCTAGGAGG CTGAAGGAGT CATTCCGGGA CCCCTACTCT
    GCCTTCTTTA AAACAGATGC TGACAGGGAT GGCATAATCA ACATGCATGA CCTTCACAGA
    CTGCTCCTGC ATCTACTGCT TAATCTCAAA GACGACGAGT TTGAGCGCTT CCTTGGCCTT
    CTTGGCTTGA GACTTAGTGT CACTTTAAAT TTTCGGGAAT TTCAAAATTT GTGTGAGAAG
    AGACCATGGA GAACAGATGA AGCGCCTCAA AGACTCATTA GACCAAAACA GAAGGTTGCG
    GATTCAGAAC TAGCTTGTGA GCAGGCTCAT CAGTATCTTG TTACCAAAGC AAAAAACAGA
    TGGTCAGACT TGTCTAAGAA TTTTCTAGAA ACCGATAATG AGGGCAATGG CATTCTTCGA
    CGCCGGGACA TAAAGAACGC ACTGTACGGT TTTGATATTC CCCTCACACC AAGAGAGTTT
    GAAAAGCTTT GGGCAAGATA CGACACCGAG GGAAAAGGGC ACATTACTTA CCAGGAATTT
    TTACAGAAAT TGGGTATTAA CTATTCGCCT GCTGTCCATC GGCCCTGTGC AGAGGATTAT
    TTCAACTTCA TGGGTCATTT TACAAAGCCA CAGCAGCTAC AGGAAGAGAT GAAGGAGCTG
    CAGCAGAGCA CAGAGAAGGC TGTGGCAGCC AGGGATAAGC TTATGGACCG CCATCAAGAT
    ATCAGCAAAG CATTCACCAA AACTGATCAA TCCAAAACCA ACTACATATC CATATGCAAG
    ATGCAGGAAG TGCTGGAAGA ATGTGGATGT TCTCTTACCG AAGGGGAGCT GACCCATCTG
    CTAAACAGTT GGGGAGTCAG CCGGCATGAT AATGCTATCA ATTACCTCGA CTTCCTGAGA
    GCAGTGGAGA ACAGCAAGTC AACAGGAGCT CAGCCCAAGG AAAAAGAAGA GAGCATGCCA
    ATCAATTTTG CAACGCTGAA TCCACAGGAG GCTGTGAGGA AGATCCAGGA AGTAGTTGAG
    TCCTCCCAGC TGGCTTTGTC CACGGCATTT TCTGCATTGG ATAAAGAGGA TACAGGATTT
    GTAAAGGCTA CAGAATTCGG ACAAGTTCTT AAGGATTTCT GTTACAAACT AACGGACAAT
    CAGTATCATT ATTTTTTGAG GAAACTAAGA ATTCATCTAA CCCCCTATAT AAATTGGAAA
    TATTTTCTCC AGAACTTTAG CTGTTTCCTT GAGGAGACAG CTGATGAGTG GGCTGAGAAA
    ATGCCCAAAG GCCCGCCGCC TACCTCTCCC AAGGCCACAG CCGACAGAGA CATCCTGGCT
    CGCCTCCACA AAGCAGTGAC TTCCCATTAC CATGCCATCA CCCAGGAGTT TGAGAATTTT
    GACACCATGA AAACGAACAC CATCTCCAGA GAGGAGTTTA GGGCCATTTG TAATCGCCGC
    GTCCAAATCC TGACGGACGA ACAGTTTGAC AGACTCTGGA ACGAGATGCC AGTCAATGCC
    AAGGGGAGGC TGAAATACCC GGACTTCCTG AGCAGGTTCA GTTCCGAGAC AGCAGCCACA
    CCAATGGCCA CTGGTGACTC GGCCGTGGCC CAGAGAGGGA GCAGTGTCCC TGACGTCTCG
    GAAGGCACCA GATCTGCCCT CTCATTGCCC ACTCAGGAGC TGAGACCAGG GTCAAAGTCG
    CAGAGCCACC CCTGTACTCC AGCGAGCACC ACCGTGATCC CGGGCACTCC ACCCTTGCAG
    AACTGTGATC CCATAGAGAG CAGGCTCCGG AAGAGAATCC AGGGCTGCTG GCGCCAGCTC
    CTGAAAGAAT GCAAGGAGAA GGACGTGGCC AGACAGGGGG ACATCAACGC CTCCGATTTC
    CTGGCTCTTG TGGAGAAATT CAACCTGGAC ATAAGCAAAG AGGAGTGTCA GCAGCTCATT
    ATAAAATACG ACTTAAAGAG CAACGGGAAA TTTGCATACT GTGACTTCAT TCAGAGCTGT
    GTCCTCCTGC TAAAAGCAAA GGAAAGCTCA CTGATGCACA GGATGAAGAT CCAGAATGCA
    CACAAGATGA AAGAAGCCGG CGCGGAGACG CCATCTTTTT ACTCTGCTCT GCTGCGTATT
    CAGCCCAAAA TTGTGCACTG CTGGAGGCCA ATGCGGCGCA CGTTCAAAAG CTATGATGAG
    GCTGGAACAG GGCTGCTAAG CGTCGCAGAT TTCAGGACGG TGAGCACAGC TCTGCGCCCA
    GTGCCTCTGC AGGGAGCAGC TGCCAAATGC CAGCGGCCAG AAACTTCCCA CGGGGAGCCC
    TCTACCTGCT GCCGAAGGCC CGGTGTGACC TGGCTGCTTC AGATCTCACT TCCCTGGGAC
    CCCCAAGTTA TCCAGTCCTG GGTCCACTCG CCTCACCTTC AGACCCCGGG TTCTCCTTCC
    CAAAGGTTGC TGGAGCCCTG CGTGGATGCC CCATACCTGC ATCGCCAGCT CCTCAGGGTC
    CTGAGACAGT ACAGCATCAA CCTCTCTGAG GAAGAGTTCT TCCATATTCT GGAGTATTAC
    GATAAGACGC TGTCTTCAAA AATCTCCTAC AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93563
    Clone ID Related Accession (Same CDS sequence) XM_017028911.2 , XM_017028911.1
    Accession Version XM_017028911.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884400.1
    CDS984..5567
    Translation

    Target ORF information:

    RefSeq Version XM_017028911.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028911.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93563
    Clone ID Related Accession (Same CDS sequence) XM_017028911.2 , XM_017028911.1
    Accession Version XM_017028911.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884400.1
    CDS276..4859
    Translation

    Target ORF information:

    RefSeq Version XM_017028911.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028911.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu32342
    Clone ID Related Accession (Same CDS sequence) NM_198856.3 , NM_198856.2
    Accession Version NM_198856.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-30
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK058069.1, AK292411.1, BC039315.1 and BP325901.1. On May 31, 2019 this sequence version replaced NM_198856.2. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: GQ901039.1, BC039315.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTC CTGAGACAGT ACAGCATCAA CCTCTCTGAG
    GAAGAGTTCT TCCATATTCT GGAGTATTAC GATAAGACGC TGTCTTCAAA AATCTCCTAC
    AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_942153.1
    CDS381..4430
    Translation

    Target ORF information:

    RefSeq Version NM_198856.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198856.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTC CTGAGACAGT ACAGCATCAA CCTCTCTGAG
    GAAGAGTTCT TCCATATTCT GGAGTATTAC GATAAGACGC TGTCTTCAAA AATCTCCTAC
    AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu32342
    Clone ID Related Accession (Same CDS sequence) NM_198856.3 , NM_198856.2
    Accession Version NM_198856.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-08
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK058069.1, AK292411.1, BC039315.1, BP325901.1 and AA778573.1. On Jul 3, 2010 this sequence version replaced NM_198856.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: BC039315.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTC CTGAGACAGT ACAGCATCAA CCTCTCTGAG
    GAAGAGTTCT TCCATATTCT GGAGTATTAC GATAAGACGC TGTCTTCAAA AATCTCCTAC
    AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_942153.1
    CDS413..4462
    Translation

    Target ORF information:

    RefSeq Version NM_198856.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198856.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTC CTGAGACAGT ACAGCATCAA CCTCTCTGAG
    GAAGAGTTCT TCCATATTCT GGAGTATTAC GATAAGACGC TGTCTTCAAA AATCTCCTAC
    AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53654
    Clone ID Related Accession (Same CDS sequence) XM_011530317.3 , XM_011530317.2 , XM_005261704.2
    Accession Version XM_011530317.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTGAGCA CAGCTCTGCG CCCAGTGCCT CTGCAGGGAG CAGCTGCCAA ATGCCAGCGG
    CCAGAAACTT CCCACGGGGA GCCCTCTACC TGCTGCCGAA GGCCCGGTGT GACCTGGCTG
    CTTCAGATCT CACTTCCCTG GGACCCCCAA GTTATCCAGT CCTGGGTCCA CTCGCCTCAC
    CTTCAGACCC CGGGTTCTCC TTCCCAAAGG TTGCTGGAGC CCTGCGTGGA TGCCCCATAC
    CTGCATCGCC AGCTCCTCAG GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528619.1
    CDS818..5581
    Translation

    Target ORF information:

    RefSeq Version XM_011530317.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530317.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTGAGCA CAGCTCTGCG CCCAGTGCCT CTGCAGGGAG CAGCTGCCAA ATGCCAGCGG
    CCAGAAACTT CCCACGGGGA GCCCTCTACC TGCTGCCGAA GGCCCGGTGT GACCTGGCTG
    CTTCAGATCT CACTTCCCTG GGACCCCCAA GTTATCCAGT CCTGGGTCCA CTCGCCTCAC
    CTTCAGACCC CGGGTTCTCC TTCCCAAAGG TTGCTGGAGC CCTGCGTGGA TGCCCCATAC
    CTGCATCGCC AGCTCCTCAG GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53654
    Clone ID Related Accession (Same CDS sequence) XM_011530317.3 , XM_011530317.2 , XM_005261704.2
    Accession Version XM_011530317.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTGAGCA CAGCTCTGCG CCCAGTGCCT CTGCAGGGAG CAGCTGCCAA ATGCCAGCGG
    CCAGAAACTT CCCACGGGGA GCCCTCTACC TGCTGCCGAA GGCCCGGTGT GACCTGGCTG
    CTTCAGATCT CACTTCCCTG GGACCCCCAA GTTATCCAGT CCTGGGTCCA CTCGCCTCAC
    CTTCAGACCC CGGGTTCTCC TTCCCAAAGG TTGCTGGAGC CCTGCGTGGA TGCCCCATAC
    CTGCATCGCC AGCTCCTCAG GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528619.1
    CDS102..4865
    Translation

    Target ORF information:

    RefSeq Version XM_011530317.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530317.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTGAGCA CAGCTCTGCG CCCAGTGCCT CTGCAGGGAG CAGCTGCCAA ATGCCAGCGG
    CCAGAAACTT CCCACGGGGA GCCCTCTACC TGCTGCCGAA GGCCCGGTGT GACCTGGCTG
    CTTCAGATCT CACTTCCCTG GGACCCCCAA GTTATCCAGT CCTGGGTCCA CTCGCCTCAC
    CTTCAGACCC CGGGTTCTCC TTCCCAAAGG TTGCTGGAGC CCTGCGTGGA TGCCCCATAC
    CTGCATCGCC AGCTCCTCAG GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53654
    Clone ID Related Accession (Same CDS sequence) XM_011530317.3 , XM_011530317.2 , XM_005261704.2
    Accession Version XM_005261704.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005261704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTGAGCA CAGCTCTGCG CCCAGTGCCT CTGCAGGGAG CAGCTGCCAA ATGCCAGCGG
    CCAGAAACTT CCCACGGGGA GCCCTCTACC TGCTGCCGAA GGCCCGGTGT GACCTGGCTG
    CTTCAGATCT CACTTCCCTG GGACCCCCAA GTTATCCAGT CCTGGGTCCA CTCGCCTCAC
    CTTCAGACCC CGGGTTCTCC TTCCCAAAGG TTGCTGGAGC CCTGCGTGGA TGCCCCATAC
    CTGCATCGCC AGCTCCTCAG GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005261761.1
    CDS255..5018
    Translation

    Target ORF information:

    RefSeq Version XM_005261704.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005261704.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTGCAAAA TGGCGATTAT ACCAGACTGG CTTAGGTCGC ATCCTCACAC ACGAAAATTT 
    ACACATTCAA GACCCCATTC TTCACCGTGT AGAGTATATT CAAGGAATGG TTCCCCAAAT
    AAGTTCAGAT CTTCTTCAAC CACAGCTGTT GCAAATCCAA CACTGTCCTC CTTAGATGTT
    AAACGGATTT TATTTCAAAA AATTACCGAC AGAGGGGATG AGTTGCAAAA AGCCTTTCAG
    CTGCTGGATA CTGGTCAGAA CTTGACTGTG TCAAAAAGTG AACTGAGAAG AATCATCACA
    GACTTCCTGA TGCCGCTCAC ACGAGAACAG TTTCAGGACG TGTTGGCTCA GATCCCCCTT
    AGCACCTCTG GTACTGTACC GTACCTTGCC TTTCTGTCCA GGTTTGGTGG AATTGACCTA
    TATATAAATG GTATAAAGAG GGGAGGTGGG AATGAAATGA ATTGCTGCCG CACATTAAGA
    GAACTTGAAA TCCAAGTGGG AGAAAAGGTT TTCAAAAACA TTAAGACTGT TATGAAAGCC
    TTTGAGCTCA TTGATGTTAA CAAGACTGGA CTGGTTCGAC CGCAGGAGCT AAGAAGGGTT
    CTGGAGACCT TCTGTATGAA GTTAAGAGAC GAGGAATACG AAAAGTTTTC GAAACACTAC
    AACATCCACA AGGATACTGC AGTAGATTAC AACGTGTTTT TGAAGAATCT CAGCATAAAT
    AACGACTTGA ACCTTAGATA TTGTATGGGA AATCAAGAGG TCTCGTTGGA GAATCAACAA
    GCCAAAAATT CCAAAAAGGA ACGTTTGCTA GGTTCTGCAT CATCTGAAGA TATCTGGAGA
    AACTACTCCT TGGATGAAAT TGAGAGGAAC TTTTGTCTAC AACTTTCGAA GTCTTATGAA
    AAGGTTGAAA AGGCCCTCAG TGCAGGGGAC CCCTGTAAAG GTGGCTACGT GTCTTTTAAT
    TATCTAAAGA TTGTCCTCGA CACTTTTGTA TACCAAATAC CAAGAAGAAT TTTTATCCAG
    TTAATGAAAA GATTTGGACT TAAAGCCACC ACTAAAATCA ATTGGAAGCA ATTTCTAACA
    TCATTTCATG AGCCTCAGGG GTTGCAAGTT AGCAGTAAAG GTCCCCTGAC AAAAAGAAAT
    AGCATCAACT CTAGAAATGA ATCTCACAAG GAAAACATCA TCACAAAGTT ATTTAGACAC
    ACTGAAGATC ACTCTGCGTC ACTGAAGAAA GCATTACTGA TAATCAACAC TAAACCCGAT
    GGACCGATAA CAAGAGAAGA ATTTCGATAT ATTCTAAATT GCATGGCTGT AAAACTAAGC
    GATTCAGAAT TCAAAGAACT AATGCAAATG CTTGACCCTG GGGACACTGG AGTGGTCAAC
    ACCAGCATGT TTATTGATCT GATTGAAGAG AACTGTAGGA TGAGAAAGAC ATCTCCCTGT
    ACAGATGCTA AGACACCTTT CCTCCTGGCC TGGGATTCAG TGGAAGAAAT TGTTCATGAT
    ACAATTACTA GGAACCTACA AGCTTTTTAT AACATGCTAC GCTCATATGA CCTTGGAGAC
    ACAGGGCGCA TTGGCCGAAA TAATTTCAAG AAAATCATGC ACGTCTTCTG TCCATTTTTA
    ACGAATGCAC ATTTCATAAA ACTCTGCAGT AAGATTCAGG ACATTGGTTC AGGAAGAATC
    CTTTACAAGA AACTTTTGGC ATGCATAGGA ATTGATGGCC CACCCACTGT CTCTCCAGTT
    CTTGTTCCAA AGGATCAGCT GTTAAGTGAA CATTTACAAA AAGATGAACA GCAGCAGCCA
    GATCTTTCTG AGAGAACCAA GCTCACGGAG GATAAAACCA CCCTGACCAA GAAGATGACC
    ACAGAAGAAG TGATTGAAAA ATTCAAAAAG TGTATACAGC AGCAGGACCC GGCATTCAAA
    AAACGATTTC TTGACTTCAG CAAGGAGCCT AATGGAAAAA TTAACGTGCA TGACTTTAAG
    AAGGTACTGG AAGACACTGG GATGCCCATG GACGATGATC AGTATGCCCT GCTGACCACT
    AAAATAGGCT TCGAGAAGGA AGGGATGAGC TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    CCTCCAATGA GAGGGCCGGA AACCACTCCG CCGCAGCCTC CAACTCCTTC AAAAAGTTAC
    GTGAACAGCC ACTTTATCAC CGCAGAAGAA TGCCTGAAGC TTTTCCCTAG GAGGCTGAAG
    GAGTCATTCC GGGACCCCTA CTCTGCCTTC TTTAAAACAG ATGCTGACAG GGATGGCATA
    ATCAACATGC ATGACCTTCA CAGACTGCTC CTGCATCTAC TGCTTAATCT CAAAGACGAC
    GAGTTTGAGC GCTTCCTTGG CCTTCTTGGC TTGAGACTTA GTGTCACTTT AAATTTTCGG
    GAATTTCAAA ATTTGTGTGA GAAGAGACCA TGGAGAACAG ATGAAGCGCC TCAAAGACTC
    ATTAGACCAA AACAGAAGGT TGCGGATTCA GAACTAGCTT GTGAGCAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAA CAGATGGTCA GACTTGTCTA AGAATTTTCT AGAAACCGAT
    AATGAGGGCA ATGGCATTCT TCGACGCCGG GACATAAAGA ACGCACTGTA CGGTTTTGAT
    ATTCCCCTCA CACCAAGAGA GTTTGAAAAG CTTTGGGCAA GATACGACAC CGAGGGAAAA
    GGGCACATTA CTTACCAGGA ATTTTTACAG AAATTGGGTA TTAACTATTC GCCTGCTGTC
    CATCGGCCCT GTGCAGAGGA TTATTTCAAC TTCATGGGTC ATTTTACAAA GCCACAGCAG
    CTACAGGAAG AGATGAAGGA GCTGCAGCAG AGCACAGAGA AGGCTGTGGC AGCCAGGGAT
    AAGCTTATGG ACCGCCATCA AGATATCAGC AAAGCATTCA CCAAAACTGA TCAATCCAAA
    ACCAACTACA TATCCATATG CAAGATGCAG GAAGTGCTGG AAGAATGTGG ATGTTCTCTT
    ACCGAAGGGG AGCTGACCCA TCTGCTAAAC AGTTGGGGAG TCAGCCGGCA TGATAATGCT
    ATCAATTACC TCGACTTCCT GAGAGCAGTG GAGAACAGCA AGTCAACAGG AGCTCAGCCC
    AAGGAAAAAG AAGAGAGCAT GCCAATCAAT TTTGCAACGC TGAATCCACA GGAGGCTGTG
    AGGAAGATCC AGGAAGTAGT TGAGTCCTCC CAGCTGGCTT TGTCCACGGC ATTTTCTGCA
    TTGGATAAAG AGGATACAGG ATTTGTAAAG GCTACAGAAT TCGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACGGA CAATCAGTAT CATTATTTTT TGAGGAAACT AAGAATTCAT
    CTAACCCCCT ATATAAATTG GAAATATTTT CTCCAGAACT TTAGCTGTTT CCTTGAGGAG
    ACAGCTGATG AGTGGGCTGA GAAAATGCCC AAAGGCCCGC CGCCTACCTC TCCCAAGGCC
    ACAGCCGACA GAGACATCCT GGCTCGCCTC CACAAAGCAG TGACTTCCCA TTACCATGCC
    ATCACCCAGG AGTTTGAGAA TTTTGACACC ATGAAAACGA ACACCATCTC CAGAGAGGAG
    TTTAGGGCCA TTTGTAATCG CCGCGTCCAA ATCCTGACGG ACGAACAGTT TGACAGACTC
    TGGAACGAGA TGCCAGTCAA TGCCAAGGGG AGGCTGAAAT ACCCGGACTT CCTGAGCAGG
    TTCAGTTCCG AGACAGCAGC CACACCAATG GCCACTGGTG ACTCGGCCGT GGCCCAGAGA
    GGGAGCAGTG TCCCTGACGT CTCGGAAGGC ACCAGATCTG CCCTCTCATT GCCCACTCAG
    GAGCTGAGAC CAGGGTCAAA GTCGCAGAGC CACCCCTGTA CTCCAGCGAG CACCACCGTG
    ATCCCGGGCA CTCCACCCTT GCAGAACTGT GATCCCATAG AGAGCAGGCT CCGGAAGAGA
    ATCCAGGGCT GCTGGCGCCA GCTCCTGAAA GAATGCAAGG AGAAGGACGT GGCCAGACAG
    GGGGACATCA ACGCCTCCGA TTTCCTGGCT CTTGTGGAGA AATTCAACCT GGACATAAGC
    AAAGAGGAGT GTCAGCAGCT CATTATAAAA TACGACTTAA AGAGCAACGG GAAATTTGCA
    TACTGTGACT TCATTCAGAG CTGTGTCCTC CTGCTAAAAG CAAAGGAAAG CTCACTGATG
    CACAGGATGA AGATCCAGAA TGCACACAAG ATGAAAGAAG CCGGCGCGGA GACGCCATCT
    TTTTACTCTG CTCTGCTGCG TATTCAGCCC AAAATTGTGC ACTGCTGGAG GCCAATGCGG
    CGCACGTTCA AAAGCTATGA TGAGGCTGGA ACAGGGCTGC TAAGCGTCGC AGATTTCAGG
    ACGGTGAGCA CAGCTCTGCG CCCAGTGCCT CTGCAGGGAG CAGCTGCCAA ATGCCAGCGG
    CCAGAAACTT CCCACGGGGA GCCCTCTACC TGCTGCCGAA GGCCCGGTGT GACCTGGCTG
    CTTCAGATCT CACTTCCCTG GGACCCCCAA GTTATCCAGT CCTGGGTCCA CTCGCCTCAC
    CTTCAGACCC CGGGTTCTCC TTCCCAAAGG TTGCTGGAGC CCTGCGTGGA TGCCCCATAC
    CTGCATCGCC AGCTCCTCAG GGTCCTGAGA CAGTACAGCA TCAACCTCTC TGAGGAAGAG
    TTCTTCCATA TTCTGGAGTA TTACGATAAG ACGCTGTCTT CAAAAATCTC CTACAACGAC
    TTCCTCCGGG CATTCCTCCA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53655
    Clone ID Related Accession (Same CDS sequence) XM_005261705.3 , XM_011530319.2 , XM_005261705.4 , XM_011530319.3
    Accession Version XM_005261705.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005261705.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAATCCCCC TTAGCACCTC TGGTACTGTA 
    CCGTACCTTG CCTTTCTGTC CAGGTTTGGT GGAATTGACC TATATATAAA TGGTATAAAG
    AGGGGAGGTG GGAATGAAAT GAATTGCTGC CGCACATTAA GAGAACTTGA AATCCAAGTG
    GGAGAAAAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005261762.1
    CDS487..4932
    Translation

    Target ORF information:

    RefSeq Version XM_005261705.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005261705.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAATCCCCC TTAGCACCTC TGGTACTGTA 
    CCGTACCTTG CCTTTCTGTC CAGGTTTGGT GGAATTGACC TATATATAAA TGGTATAAAG
    AGGGGAGGTG GGAATGAAAT GAATTGCTGC CGCACATTAA GAGAACTTGA AATCCAAGTG
    GGAGAAAAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53655
    Clone ID Related Accession (Same CDS sequence) XM_005261705.3 , XM_011530319.2 , XM_005261705.4 , XM_011530319.3
    Accession Version XM_011530319.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530319.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAATCCCCC TTAGCACCTC TGGTACTGTA 
    CCGTACCTTG CCTTTCTGTC CAGGTTTGGT GGAATTGACC TATATATAAA TGGTATAAAG
    AGGGGAGGTG GGAATGAAAT GAATTGCTGC CGCACATTAA GAGAACTTGA AATCCAAGTG
    GGAGAAAAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528621.1
    CDS208..4653
    Translation

    Target ORF information:

    RefSeq Version XM_011530319.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530319.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAATCCCCC TTAGCACCTC TGGTACTGTA 
    CCGTACCTTG CCTTTCTGTC CAGGTTTGGT GGAATTGACC TATATATAAA TGGTATAAAG
    AGGGGAGGTG GGAATGAAAT GAATTGCTGC CGCACATTAA GAGAACTTGA AATCCAAGTG
    GGAGAAAAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53655
    Clone ID Related Accession (Same CDS sequence) XM_005261705.3 , XM_011530319.2 , XM_005261705.4 , XM_011530319.3
    Accession Version XM_005261705.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005261705.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAATCCCCC TTAGCACCTC TGGTACTGTA 
    CCGTACCTTG CCTTTCTGTC CAGGTTTGGT GGAATTGACC TATATATAAA TGGTATAAAG
    AGGGGAGGTG GGAATGAAAT GAATTGCTGC CGCACATTAA GAGAACTTGA AATCCAAGTG
    GGAGAAAAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005261762.1
    CDS1061..5506
    Translation

    Target ORF information:

    RefSeq Version XM_005261705.4
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005261705.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAATCCCCC TTAGCACCTC TGGTACTGTA 
    CCGTACCTTG CCTTTCTGTC CAGGTTTGGT GGAATTGACC TATATATAAA TGGTATAAAG
    AGGGGAGGTG GGAATGAAAT GAATTGCTGC CGCACATTAA GAGAACTTGA AATCCAAGTG
    GGAGAAAAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53655
    Clone ID Related Accession (Same CDS sequence) XM_005261705.3 , XM_011530319.2 , XM_005261705.4 , XM_011530319.3
    Accession Version XM_011530319.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530319.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAATCCCCC TTAGCACCTC TGGTACTGTA 
    CCGTACCTTG CCTTTCTGTC CAGGTTTGGT GGAATTGACC TATATATAAA TGGTATAAAG
    AGGGGAGGTG GGAATGAAAT GAATTGCTGC CGCACATTAA GAGAACTTGA AATCCAAGTG
    GGAGAAAAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528621.1
    CDS924..5369
    Translation

    Target ORF information:

    RefSeq Version XM_011530319.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530319.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAATCCCCC TTAGCACCTC TGGTACTGTA 
    CCGTACCTTG CCTTTCTGTC CAGGTTTGGT GGAATTGACC TATATATAAA TGGTATAAAG
    AGGGGAGGTG GGAATGAAAT GAATTGCTGC CGCACATTAA GAGAACTTGA AATCCAAGTG
    GGAGAAAAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71501
    Clone ID Related Accession (Same CDS sequence) XM_017028910.1 , XM_017028910.2 , XM_011530316.1
    Accession Version XM_017028910.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTGAGCACA GCTCTGCGCC CAGTGCCTCT GCAGGGAGCA
    GCTGCCAAAT GCCAGCGGCC AGAAACTTCC CACGGGGAGC CCTCTACCTG CTGCCGAAGG
    CCCGGTGTGA CCTGGCTGCT TCAGATCTCA CTTCCCTGGG ACCCCCAAGT TATCCAGTCC
    TGGGTCCACT CGCCTCACCT TCAGACCCCG GGTTCTCCTT CCCAAAGGTT GCTGGAGCCC
    TGCGTGGATG CCCCATACCT GCATCGCCAG CTCCTCAGGG TCCTGAGACA GTACAGCATC
    AACCTCTCTG AGGAAGAGTT CTTCCATATT CTGGAGTATT ACGATAAGAC GCTGTCTTCA
    AAAATCTCCT ACAACGACTT CCTCCGGGCA TTCCTCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884399.1
    CDS276..5117
    Translation

    Target ORF information:

    RefSeq Version XM_017028910.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028910.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTGAGCACA GCTCTGCGCC CAGTGCCTCT GCAGGGAGCA
    GCTGCCAAAT GCCAGCGGCC AGAAACTTCC CACGGGGAGC CCTCTACCTG CTGCCGAAGG
    CCCGGTGTGA CCTGGCTGCT TCAGATCTCA CTTCCCTGGG ACCCCCAAGT TATCCAGTCC
    TGGGTCCACT CGCCTCACCT TCAGACCCCG GGTTCTCCTT CCCAAAGGTT GCTGGAGCCC
    TGCGTGGATG CCCCATACCT GCATCGCCAG CTCCTCAGGG TCCTGAGACA GTACAGCATC
    AACCTCTCTG AGGAAGAGTT CTTCCATATT CTGGAGTATT ACGATAAGAC GCTGTCTTCA
    AAAATCTCCT ACAACGACTT CCTCCGGGCA TTCCTCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71501
    Clone ID Related Accession (Same CDS sequence) XM_017028910.1 , XM_017028910.2 , XM_011530316.1
    Accession Version XM_017028910.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTGAGCACA GCTCTGCGCC CAGTGCCTCT GCAGGGAGCA
    GCTGCCAAAT GCCAGCGGCC AGAAACTTCC CACGGGGAGC CCTCTACCTG CTGCCGAAGG
    CCCGGTGTGA CCTGGCTGCT TCAGATCTCA CTTCCCTGGG ACCCCCAAGT TATCCAGTCC
    TGGGTCCACT CGCCTCACCT TCAGACCCCG GGTTCTCCTT CCCAAAGGTT GCTGGAGCCC
    TGCGTGGATG CCCCATACCT GCATCGCCAG CTCCTCAGGG TCCTGAGACA GTACAGCATC
    AACCTCTCTG AGGAAGAGTT CTTCCATATT CTGGAGTATT ACGATAAGAC GCTGTCTTCA
    AAAATCTCCT ACAACGACTT CCTCCGGGCA TTCCTCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884399.1
    CDS984..5825
    Translation

    Target ORF information:

    RefSeq Version XM_017028910.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028910.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTGAGCACA GCTCTGCGCC CAGTGCCTCT GCAGGGAGCA
    GCTGCCAAAT GCCAGCGGCC AGAAACTTCC CACGGGGAGC CCTCTACCTG CTGCCGAAGG
    CCCGGTGTGA CCTGGCTGCT TCAGATCTCA CTTCCCTGGG ACCCCCAAGT TATCCAGTCC
    TGGGTCCACT CGCCTCACCT TCAGACCCCG GGTTCTCCTT CCCAAAGGTT GCTGGAGCCC
    TGCGTGGATG CCCCATACCT GCATCGCCAG CTCCTCAGGG TCCTGAGACA GTACAGCATC
    AACCTCTCTG AGGAAGAGTT CTTCCATATT CTGGAGTATT ACGATAAGAC GCTGTCTTCA
    AAAATCTCCT ACAACGACTT CCTCCGGGCA TTCCTCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71501
    Clone ID Related Accession (Same CDS sequence) XM_017028910.1 , XM_017028910.2 , XM_011530316.1
    Accession Version XM_011530316.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTGAGCACA GCTCTGCGCC CAGTGCCTCT GCAGGGAGCA
    GCTGCCAAAT GCCAGCGGCC AGAAACTTCC CACGGGGAGC CCTCTACCTG CTGCCGAAGG
    CCCGGTGTGA CCTGGCTGCT TCAGATCTCA CTTCCCTGGG ACCCCCAAGT TATCCAGTCC
    TGGGTCCACT CGCCTCACCT TCAGACCCCG GGTTCTCCTT CCCAAAGGTT GCTGGAGCCC
    TGCGTGGATG CCCCATACCT GCATCGCCAG CTCCTCAGGG TCCTGAGACA GTACAGCATC
    AACCTCTCTG AGGAAGAGTT CTTCCATATT CTGGAGTATT ACGATAAGAC GCTGTCTTCA
    AAAATCTCCT ACAACGACTT CCTCCGGGCA TTCCTCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528618.1
    CDS464..5305
    Translation

    Target ORF information:

    RefSeq Version XM_011530316.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530316.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTGAGCACA GCTCTGCGCC CAGTGCCTCT GCAGGGAGCA
    GCTGCCAAAT GCCAGCGGCC AGAAACTTCC CACGGGGAGC CCTCTACCTG CTGCCGAAGG
    CCCGGTGTGA CCTGGCTGCT TCAGATCTCA CTTCCCTGGG ACCCCCAAGT TATCCAGTCC
    TGGGTCCACT CGCCTCACCT TCAGACCCCG GGTTCTCCTT CCCAAAGGTT GCTGGAGCCC
    TGCGTGGATG CCCCATACCT GCATCGCCAG CTCCTCAGGG TCCTGAGACA GTACAGCATC
    AACCTCTCTG AGGAAGAGTT CTTCCATATT CTGGAGTATT ACGATAAGAC GCTGTCTTCA
    AAAATCTCCT ACAACGACTT CCTCCGGGCA TTCCTCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71502
    Clone ID Related Accession (Same CDS sequence) XM_011530318.2 , XM_011530318.3
    Accession Version XM_011530318.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528620.1
    CDS276..4961
    Translation

    Target ORF information:

    RefSeq Version XM_011530318.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71502
    Clone ID Related Accession (Same CDS sequence) XM_011530318.2 , XM_011530318.3
    Accession Version XM_011530318.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528620.1
    CDS984..5669
    Translation

    Target ORF information:

    RefSeq Version XM_011530318.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530318.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGG TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT
    AACAAGACTG GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG
    AAGTTAAGAG ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT
    GCAGTAGATT ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA
    TATTGTATGG GAAATCAAGA GGTCTCGTTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG
    GAACGTTTGC TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA
    ATTGAGAGGA ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC
    AGTGCAGGGG ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC
    GACACTTTTG TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA
    CTTAAAGCCA CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG
    GGGTTGCAAG TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT
    GAATCTCACA AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG
    TCACTGAAGA AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA
    GAATTTCGAT ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA
    CTAATGCAAA TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT
    CTGATTGAAG AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT
    TTCCTCCTGG CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA
    CAAGCTTTTT ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA
    AATAATTTCA AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA
    AAACTCTGCA GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG
    GCATGCATAG GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG
    CTGTTAAGTG AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC
    AAGCTCACGG AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA
    AAATTCAAAA AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC
    AGCAAGGAGC CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT
    GGGATGCCCA TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG
    GAAGGGATGA GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG
    GAAACCACTC CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC
    ACCGCAGAAG AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC
    TACTCTGCCT TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT
    CACAGACTGC TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT
    GGCCTTCTTG GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT
    GAGAAGAGAC CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG
    GTTGCGGATT CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA
    AACAGATGGT CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT
    CTTCGACGCC GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA
    GAGTTTGAAA AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG
    GAATTTTTAC AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG
    GATTATTTCA ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG
    GAGCTGCAGC AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT
    CAAGATATCA GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA
    TGCAAGATGC AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC
    CATCTGCTAA ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC
    CTGAGAGCAG TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC
    ATGCCAATCA ATTTTGCAAC GCTGAATCCA CAGGAGGCTG TGAGGAAGAT CCAGGAAGTA
    GTTGAGTCCT CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGATAA AGAGGATACA
    GGATTTGTAA AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG
    GACAATCAGT ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT
    TGGAAATATT TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT
    GAGAAAATGC CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC
    CTGGCTCGCC TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG
    AATTTTGACA CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT
    CGCCGCGTCC AAATCCTGAC GGACGAACAG TTTGACAGAC TCTGGAACGA GATGCCAGTC
    AATGCCAAGG GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA
    GCCACACCAA TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC
    GTCTCGGAAG GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA
    AAGTCGCAGA GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC
    TTGCAGAACT GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC
    CAGCTCCTGA AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC
    GATTTCCTGG CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG
    CTCATTATAA AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG
    AGCTGTGTCC TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG
    AATGCACACA AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG
    CGTATTCAGC CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT
    GATGAGGCTG GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTGAG CACAGCTCTG
    CGCCCAGTGC CTCTGCAGGG AGCAGCTGCC AAATGCCAGC GGCCAGAAAC TTCCCACGGG
    GAGCCCTCTA CCTGCTGCCG AAGGCCCGGT GTGACCTGGC TGCTTCAGAT CTCACTTCCC
    TGGGACCCCC AAGTTATCCA GTCCTGGGTC CACTCGCCTC ACCTTCAGAC CCCGGGTTCT
    CCTTCCCAAA GGTTGCTGGA GCCCTGCGTG GATGCCCCAT ACCTGCATCG CCAGCTCCTC
    AGGGTCCTGA GACAGTACAG CATCAACCTC TCTGAGGAAG AGTTCTTCCA TATTCTGGAG
    TATTACGATA AGACGCTGTC TTCAAAAATC TCCTACAACG ACTTCCTCCG GGCATTCCTC
    CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71503
    Clone ID Related Accession (Same CDS sequence) XM_011530320.2 , XM_011530320.3
    Accession Version XM_011530320.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAGCCGGCG
    CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT GTGCACTGCT
    GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528622.1
    CDS276..4712
    Translation

    Target ORF information:

    RefSeq Version XM_011530320.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530320.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAGCCGGCG
    CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT GTGCACTGCT
    GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71503
    Clone ID Related Accession (Same CDS sequence) XM_011530320.2 , XM_011530320.3
    Accession Version XM_011530320.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530320.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAGCCGGCG
    CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT GTGCACTGCT
    GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528622.1
    CDS984..5420
    Translation

    Target ORF information:

    RefSeq Version XM_011530320.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530320.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAACGAA ATAGCACCAA AACAAATGCT CCCAAAGGCA GCAGCACCCT CTCAGGCAGT 
    ACCAAAGCCA GGGATTTAAT GTGCAAAATG GCGATTATAC CAGACTGGCT TAGGTCGCAT
    CCTCACACAC GAAAATTTAC ACATTCAAGA CCCCATTCTT CACCGTGTAG AGTATATTCA
    AGGAATGGTT CCCCAAATAA GTTCAGATCT TCTTCAACCA CAGCTGTTGC AAATCCAACA
    CTGTCCTCCT TAGATGTTAA ACGGATTTTA TTTCAAAAAA TTACCGACAG AGGGGATGAG
    TTGCAAAAAG CCTTTCAGCT GCTGGATACT GGTCAGAACT TGACTGTGTC AAAAAGTGAA
    CTGAGAAGAA TCATCACAGA CTTCCTGATG CCGCTCACAC GAGAACAGTT TCAGGACGTG
    TTGGCTCAGA TCCCCCTTAG CACCTCTGGT ACTGTACCGT ACCTTGCCTT TCTGTCCAGG
    TTTGGTGGAA TTGACCTATA TATAAATGGT ATAAAGAGGG GAGGTGGGAA TGAAATGAAT
    TGCTGCCGCA CATTAAGAGA ACTTGAAATC CAAGTGGGAG AAAAGGTTTT CAAAAACATT
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAGCCGGCG
    CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT GTGCACTGCT
    GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG CTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71504
    Clone ID Related Accession (Same CDS sequence) XM_011530321.3 , XM_011530321.2 , XM_011530322.2 , XM_024452262.1
    Accession Version XM_011530321.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530321.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTG AGCACAGCTC TGCGCCCAGT GCCTCTGCAG
    GGAGCAGCTG CCAAATGCCA GCGGCCAGAA ACTTCCCACG GGGAGCCCTC TACCTGCTGC
    CGAAGGCCCG GTGTGACCTG GCTGCTTCAG ATCTCACTTC CCTGGGACCC CCAAGTTATC
    CAGTCCTGGG TCCACTCGCC TCACCTTCAG ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG
    GAGCCCTGCG TGGATGCCCC ATACCTGCAT CGCCAGCTCC TCAGGGTCCT GAGACAGTAC
    AGCATCAACC TCTCTGAGGA AGAGTTCTTC CATATTCTGG AGTATTACGA TAAGACGCTG
    TCTTCAAAAA TCTCCTACAA CGACTTCCTC CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528623.1
    CDS1053..5360
    Translation

    Target ORF information:

    RefSeq Version XM_011530321.3
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530321.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTG AGCACAGCTC TGCGCCCAGT GCCTCTGCAG
    GGAGCAGCTG CCAAATGCCA GCGGCCAGAA ACTTCCCACG GGGAGCCCTC TACCTGCTGC
    CGAAGGCCCG GTGTGACCTG GCTGCTTCAG ATCTCACTTC CCTGGGACCC CCAAGTTATC
    CAGTCCTGGG TCCACTCGCC TCACCTTCAG ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG
    GAGCCCTGCG TGGATGCCCC ATACCTGCAT CGCCAGCTCC TCAGGGTCCT GAGACAGTAC
    AGCATCAACC TCTCTGAGGA AGAGTTCTTC CATATTCTGG AGTATTACGA TAAGACGCTG
    TCTTCAAAAA TCTCCTACAA CGACTTCCTC CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71504
    Clone ID Related Accession (Same CDS sequence) XM_011530321.3 , XM_011530321.2 , XM_011530322.2 , XM_024452262.1
    Accession Version XM_011530321.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTG AGCACAGCTC TGCGCCCAGT GCCTCTGCAG
    GGAGCAGCTG CCAAATGCCA GCGGCCAGAA ACTTCCCACG GGGAGCCCTC TACCTGCTGC
    CGAAGGCCCG GTGTGACCTG GCTGCTTCAG ATCTCACTTC CCTGGGACCC CCAAGTTATC
    CAGTCCTGGG TCCACTCGCC TCACCTTCAG ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG
    GAGCCCTGCG TGGATGCCCC ATACCTGCAT CGCCAGCTCC TCAGGGTCCT GAGACAGTAC
    AGCATCAACC TCTCTGAGGA AGAGTTCTTC CATATTCTGG AGTATTACGA TAAGACGCTG
    TCTTCAAAAA TCTCCTACAA CGACTTCCTC CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528623.1
    CDS341..4648
    Translation

    Target ORF information:

    RefSeq Version XM_011530321.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530321.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTG AGCACAGCTC TGCGCCCAGT GCCTCTGCAG
    GGAGCAGCTG CCAAATGCCA GCGGCCAGAA ACTTCCCACG GGGAGCCCTC TACCTGCTGC
    CGAAGGCCCG GTGTGACCTG GCTGCTTCAG ATCTCACTTC CCTGGGACCC CCAAGTTATC
    CAGTCCTGGG TCCACTCGCC TCACCTTCAG ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG
    GAGCCCTGCG TGGATGCCCC ATACCTGCAT CGCCAGCTCC TCAGGGTCCT GAGACAGTAC
    AGCATCAACC TCTCTGAGGA AGAGTTCTTC CATATTCTGG AGTATTACGA TAAGACGCTG
    TCTTCAAAAA TCTCCTACAA CGACTTCCTC CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71504
    Clone ID Related Accession (Same CDS sequence) XM_011530321.3 , XM_011530321.2 , XM_011530322.2 , XM_024452262.1
    Accession Version XM_011530322.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTG AGCACAGCTC TGCGCCCAGT GCCTCTGCAG
    GGAGCAGCTG CCAAATGCCA GCGGCCAGAA ACTTCCCACG GGGAGCCCTC TACCTGCTGC
    CGAAGGCCCG GTGTGACCTG GCTGCTTCAG ATCTCACTTC CCTGGGACCC CCAAGTTATC
    CAGTCCTGGG TCCACTCGCC TCACCTTCAG ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG
    GAGCCCTGCG TGGATGCCCC ATACCTGCAT CGCCAGCTCC TCAGGGTCCT GAGACAGTAC
    AGCATCAACC TCTCTGAGGA AGAGTTCTTC CATATTCTGG AGTATTACGA TAAGACGCTG
    TCTTCAAAAA TCTCCTACAA CGACTTCCTC CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528624.1
    CDS469..4776
    Translation

    Target ORF information:

    RefSeq Version XM_011530322.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530322.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTG AGCACAGCTC TGCGCCCAGT GCCTCTGCAG
    GGAGCAGCTG CCAAATGCCA GCGGCCAGAA ACTTCCCACG GGGAGCCCTC TACCTGCTGC
    CGAAGGCCCG GTGTGACCTG GCTGCTTCAG ATCTCACTTC CCTGGGACCC CCAAGTTATC
    CAGTCCTGGG TCCACTCGCC TCACCTTCAG ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG
    GAGCCCTGCG TGGATGCCCC ATACCTGCAT CGCCAGCTCC TCAGGGTCCT GAGACAGTAC
    AGCATCAACC TCTCTGAGGA AGAGTTCTTC CATATTCTGG AGTATTACGA TAAGACGCTG
    TCTTCAAAAA TCTCCTACAA CGACTTCCTC CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71504
    Clone ID Related Accession (Same CDS sequence) XM_011530321.3 , XM_011530321.2 , XM_011530322.2 , XM_024452262.1
    Accession Version XM_024452262.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTG AGCACAGCTC TGCGCCCAGT GCCTCTGCAG
    GGAGCAGCTG CCAAATGCCA GCGGCCAGAA ACTTCCCACG GGGAGCCCTC TACCTGCTGC
    CGAAGGCCCG GTGTGACCTG GCTGCTTCAG ATCTCACTTC CCTGGGACCC CCAAGTTATC
    CAGTCCTGGG TCCACTCGCC TCACCTTCAG ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG
    GAGCCCTGCG TGGATGCCCC ATACCTGCAT CGCCAGCTCC TCAGGGTCCT GAGACAGTAC
    AGCATCAACC TCTCTGAGGA AGAGTTCTTC CATATTCTGG AGTATTACGA TAAGACGCTG
    TCTTCAAAAA TCTCCTACAA CGACTTCCTC CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308030.1
    CDS1646..5953
    Translation

    Target ORF information:

    RefSeq Version XM_024452262.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452262.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATTGCT GCCGCACATT AAGAGAACTT GAAATCCAAG TGGGAGAAAA GGTTTTCAAA 
    AACATTAAGA CTGTTATGAA AGCCTTTGAG CTCATTGATG TTAACAAGAC TGGACTGGTT
    CGACCGCAGG AGCTAAGAAG GGTTCTGGAG ACCTTCTGTA TGAAGTTAAG AGACGAGGAA
    TACGAAAAGT TTTCGAAACA CTACAACATC CACAAGGATA CTGCAGTAGA TTACAACGTG
    TTTTTGAAGA ATCTCAGCAT AAATAACGAC TTGAACCTTA GATATTGTAT GGGAAATCAA
    GAGGTCTCGT TGGAGAATCA ACAAGCCAAA AATTCCAAAA AGGAACGTTT GCTAGGTTCT
    GCATCATCTG AAGATATCTG GAGAAACTAC TCCTTGGATG AAATTGAGAG GAACTTTTGT
    CTACAACTTT CGAAGTCTTA TGAAAAGGTT GAAAAGGCCC TCAGTGCAGG GGACCCCTGT
    AAAGGTGGCT ACGTGTCTTT TAATTATCTA AAGATTGTCC TCGACACTTT TGTATACCAA
    ATACCAAGAA GAATTTTTAT CCAGTTAATG AAAAGATTTG GACTTAAAGC CACCACTAAA
    ATCAATTGGA AGCAATTTCT AACATCATTT CATGAGCCTC AGGGGTTGCA AGTTAGCAGT
    AAAGGTCCCC TGACAAAAAG AAATAGCATC AACTCTAGAA ATGAATCTCA CAAGGAAAAC
    ATCATCACAA AGTTATTTAG ACACACTGAA GATCACTCTG CGTCACTGAA GAAAGCATTA
    CTGATAATCA ACACTAAACC CGATGGACCG ATAACAAGAG AAGAATTTCG ATATATTCTA
    AATTGCATGG CTGTAAAACT AAGCGATTCA GAATTCAAAG AACTAATGCA AATGCTTGAC
    CCTGGGGACA CTGGAGTGGT CAACACCAGC ATGTTTATTG ATCTGATTGA AGAGAACTGT
    AGGATGAGAA AGACATCTCC CTGTACAGAT GCTAAGACAC CTTTCCTCCT GGCCTGGGAT
    TCAGTGGAAG AAATTGTTCA TGATACAATT ACTAGGAACC TACAAGCTTT TTATAACATG
    CTACGCTCAT ATGACCTTGG AGACACAGGG CGCATTGGCC GAAATAATTT CAAGAAAATC
    ATGCACGTCT TCTGTCCATT TTTAACGAAT GCACATTTCA TAAAACTCTG CAGTAAGATT
    CAGGACATTG GTTCAGGAAG AATCCTTTAC AAGAAACTTT TGGCATGCAT AGGAATTGAT
    GGCCCACCCA CTGTCTCTCC AGTTCTTGTT CCAAAGGATC AGCTGTTAAG TGAACATTTA
    CAAAAAGATG AACAGCAGCA GCCAGATCTT TCTGAGAGAA CCAAGCTCAC GGAGGATAAA
    ACCACCCTGA CCAAGAAGAT GACCACAGAA GAAGTGATTG AAAAATTCAA AAAGTGTATA
    CAGCAGCAGG ACCCGGCATT CAAAAAACGA TTTCTTGACT TCAGCAAGGA GCCTAATGGA
    AAAATTAACG TGCATGACTT TAAGAAGGTA CTGGAAGACA CTGGGATGCC CATGGACGAT
    GATCAGTATG CCCTGCTGAC CACTAAAATA GGCTTCGAGA AGGAAGGGAT GAGCTATCTT
    GATTTTGCAG CAGGATTTGA AGATCCTCCA ATGAGAGGGC CGGAAACCAC TCCGCCGCAG
    CCTCCAACTC CTTCAAAAAG TTACGTGAAC AGCCACTTTA TCACCGCAGA AGAATGCCTG
    AAGCTTTTCC CTAGGAGGCT GAAGGAGTCA TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ACAGATGCTG ACAGGGATGG CATAATCAAC ATGCATGACC TTCACAGACT GCTCCTGCAT
    CTACTGCTTA ATCTCAAAGA CGACGAGTTT GAGCGCTTCC TTGGCCTTCT TGGCTTGAGA
    CTTAGTGTCA CTTTAAATTT TCGGGAATTT CAAAATTTGT GTGAGAAGAG ACCATGGAGA
    ACAGATGAAG CGCCTCAAAG ACTCATTAGA CCAAAACAGA AGGTTGCGGA TTCAGAACTA
    GCTTGTGAGC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAAACAGATG GTCAGACTTG
    TCTAAGAATT TTCTAGAAAC CGATAATGAG GGCAATGGCA TTCTTCGACG CCGGGACATA
    AAGAACGCAC TGTACGGTTT TGATATTCCC CTCACACCAA GAGAGTTTGA AAAGCTTTGG
    GCAAGATACG ACACCGAGGG AAAAGGGCAC ATTACTTACC AGGAATTTTT ACAGAAATTG
    GGTATTAACT ATTCGCCTGC TGTCCATCGG CCCTGTGCAG AGGATTATTT CAACTTCATG
    GGTCATTTTA CAAAGCCACA GCAGCTACAG GAAGAGATGA AGGAGCTGCA GCAGAGCACA
    GAGAAGGCTG TGGCAGCCAG GGATAAGCTT ATGGACCGCC ATCAAGATAT CAGCAAAGCA
    TTCACCAAAA CTGATCAATC CAAAACCAAC TACATATCCA TATGCAAGAT GCAGGAAGTG
    CTGGAAGAAT GTGGATGTTC TCTTACCGAA GGGGAGCTGA CCCATCTGCT AAACAGTTGG
    GGAGTCAGCC GGCATGATAA TGCTATCAAT TACCTCGACT TCCTGAGAGC AGTGGAGAAC
    AGCAAGTCAA CAGGAGCTCA GCCCAAGGAA AAAGAAGAGA GCATGCCAAT CAATTTTGCA
    ACGCTGAATC CACAGGAGGC TGTGAGGAAG ATCCAGGAAG TAGTTGAGTC CTCCCAGCTG
    GCTTTGTCCA CGGCATTTTC TGCATTGGAT AAAGAGGATA CAGGATTTGT AAAGGCTACA
    GAATTCGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CGGACAATCA GTATCATTAT
    TTTTTGAGGA AACTAAGAAT TCATCTAACC CCCTATATAA ATTGGAAATA TTTTCTCCAG
    AACTTTAGCT GTTTCCTTGA GGAGACAGCT GATGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCGCCGCCTA CCTCTCCCAA GGCCACAGCC GACAGAGACA TCCTGGCTCG CCTCCACAAA
    GCAGTGACTT CCCATTACCA TGCCATCACC CAGGAGTTTG AGAATTTTGA CACCATGAAA
    ACGAACACCA TCTCCAGAGA GGAGTTTAGG GCCATTTGTA ATCGCCGCGT CCAAATCCTG
    ACGGACGAAC AGTTTGACAG ACTCTGGAAC GAGATGCCAG TCAATGCCAA GGGGAGGCTG
    AAATACCCGG ACTTCCTGAG CAGGTTCAGT TCCGAGACAG CAGCCACACC AATGGCCACT
    GGTGACTCGG CCGTGGCCCA GAGAGGGAGC AGTGTCCCTG ACGTCTCGGA AGGCACCAGA
    TCTGCCCTCT CATTGCCCAC TCAGGAGCTG AGACCAGGGT CAAAGTCGCA GAGCCACCCC
    TGTACTCCAG CGAGCACCAC CGTGATCCCG GGCACTCCAC CCTTGCAGAA CTGTGATCCC
    ATAGAGAGCA GGCTCCGGAA GAGAATCCAG GGCTGCTGGC GCCAGCTCCT GAAAGAATGC
    AAGGAGAAGG ACGTGGCCAG ACAGGGGGAC ATCAACGCCT CCGATTTCCT GGCTCTTGTG
    GAGAAATTCA ACCTGGACAT AAGCAAAGAG GAGTGTCAGC AGCTCATTAT AAAATACGAC
    TTAAAGAGCA ACGGGAAATT TGCATACTGT GACTTCATTC AGAGCTGTGT CCTCCTGCTA
    AAAGCAAAGG AAAGCTCACT GATGCACAGG ATGAAGATCC AGAATGCACA CAAGATGAAA
    GAAGCCGGCG CGGAGACGCC ATCTTTTTAC TCTGCTCTGC TGCGTATTCA GCCCAAAATT
    GTGCACTGCT GGAGGCCAAT GCGGCGCACG TTCAAAAGCT ATGATGAGGC TGGAACAGGG
    CTGCTAAGCG TCGCAGATTT CAGGACGGTG AGCACAGCTC TGCGCCCAGT GCCTCTGCAG
    GGAGCAGCTG CCAAATGCCA GCGGCCAGAA ACTTCCCACG GGGAGCCCTC TACCTGCTGC
    CGAAGGCCCG GTGTGACCTG GCTGCTTCAG ATCTCACTTC CCTGGGACCC CCAAGTTATC
    CAGTCCTGGG TCCACTCGCC TCACCTTCAG ACCCCGGGTT CTCCTTCCCA AAGGTTGCTG
    GAGCCCTGCG TGGATGCCCC ATACCTGCAT CGCCAGCTCC TCAGGGTCCT GAGACAGTAC
    AGCATCAACC TCTCTGAGGA AGAGTTCTTC CATATTCTGG AGTATTACGA TAAGACGCTG
    TCTTCAAAAA TCTCCTACAA CGACTTCCTC CGGGCATTCC TCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71505
    Clone ID Related Accession (Same CDS sequence) XM_011530323.2
    Accession Version XM_011530323.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCATTTG AAGAGGAGTT TGGAGAGAAG ATAAGAGGAT TGGGGGTTTT CAAAAACATT 
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTGAGCACA GCTCTGCGCC CAGTGCCTCT GCAGGGAGCA
    GCTGCCAAAT GCCAGCGGCC AGAAACTTCC CACGGGGAGC CCTCTACCTG CTGCCGAAGG
    CCCGGTGTGA CCTGGCTGCT TCAGATCTCA CTTCCCTGGG ACCCCCAAGT TATCCAGTCC
    TGGGTCCACT CGCCTCACCT TCAGACCCCG GGTTCTCCTT CCCAAAGGTT GCTGGAGCCC
    TGCGTGGATG CCCCATACCT GCATCGCCAG CTCCTCAGGG TCCTGAGACA GTACAGCATC
    AACCTCTCTG AGGAAGAGTT CTTCCATATT CTGGAGTATT ACGATAAGAC GCTGTCTTCA
    AAAATCTCCT ACAACGACTT CCTCCGGGCA TTCCTCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528625.1
    CDS133..4434
    Translation

    Target ORF information:

    RefSeq Version XM_011530323.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530323.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCATTTG AAGAGGAGTT TGGAGAGAAG ATAAGAGGAT TGGGGGTTTT CAAAAACATT 
    AAGACTGTTA TGAAAGCCTT TGAGCTCATT GATGTTAACA AGACTGGACT GGTTCGACCG
    CAGGAGCTAA GAAGGGTTCT GGAGACCTTC TGTATGAAGT TAAGAGACGA GGAATACGAA
    AAGTTTTCGA AACACTACAA CATCCACAAG GATACTGCAG TAGATTACAA CGTGTTTTTG
    AAGAATCTCA GCATAAATAA CGACTTGAAC CTTAGATATT GTATGGGAAA TCAAGAGGTC
    TCGTTGGAGA ATCAACAAGC CAAAAATTCC AAAAAGGAAC GTTTGCTAGG TTCTGCATCA
    TCTGAAGATA TCTGGAGAAA CTACTCCTTG GATGAAATTG AGAGGAACTT TTGTCTACAA
    CTTTCGAAGT CTTATGAAAA GGTTGAAAAG GCCCTCAGTG CAGGGGACCC CTGTAAAGGT
    GGCTACGTGT CTTTTAATTA TCTAAAGATT GTCCTCGACA CTTTTGTATA CCAAATACCA
    AGAAGAATTT TTATCCAGTT AATGAAAAGA TTTGGACTTA AAGCCACCAC TAAAATCAAT
    TGGAAGCAAT TTCTAACATC ATTTCATGAG CCTCAGGGGT TGCAAGTTAG CAGTAAAGGT
    CCCCTGACAA AAAGAAATAG CATCAACTCT AGAAATGAAT CTCACAAGGA AAACATCATC
    ACAAAGTTAT TTAGACACAC TGAAGATCAC TCTGCGTCAC TGAAGAAAGC ATTACTGATA
    ATCAACACTA AACCCGATGG ACCGATAACA AGAGAAGAAT TTCGATATAT TCTAAATTGC
    ATGGCTGTAA AACTAAGCGA TTCAGAATTC AAAGAACTAA TGCAAATGCT TGACCCTGGG
    GACACTGGAG TGGTCAACAC CAGCATGTTT ATTGATCTGA TTGAAGAGAA CTGTAGGATG
    AGAAAGACAT CTCCCTGTAC AGATGCTAAG ACACCTTTCC TCCTGGCCTG GGATTCAGTG
    GAAGAAATTG TTCATGATAC AATTACTAGG AACCTACAAG CTTTTTATAA CATGCTACGC
    TCATATGACC TTGGAGACAC AGGGCGCATT GGCCGAAATA ATTTCAAGAA AATCATGCAC
    GTCTTCTGTC CATTTTTAAC GAATGCACAT TTCATAAAAC TCTGCAGTAA GATTCAGGAC
    ATTGGTTCAG GAAGAATCCT TTACAAGAAA CTTTTGGCAT GCATAGGAAT TGATGGCCCA
    CCCACTGTCT CTCCAGTTCT TGTTCCAAAG GATCAGCTGT TAAGTGAACA TTTACAAAAA
    GATGAACAGC AGCAGCCAGA TCTTTCTGAG AGAACCAAGC TCACGGAGGA TAAAACCACC
    CTGACCAAGA AGATGACCAC AGAAGAAGTG ATTGAAAAAT TCAAAAAGTG TATACAGCAG
    CAGGACCCGG CATTCAAAAA ACGATTTCTT GACTTCAGCA AGGAGCCTAA TGGAAAAATT
    AACGTGCATG ACTTTAAGAA GGTACTGGAA GACACTGGGA TGCCCATGGA CGATGATCAG
    TATGCCCTGC TGACCACTAA AATAGGCTTC GAGAAGGAAG GGATGAGCTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATCC TCCAATGAGA GGGCCGGAAA CCACTCCGCC GCAGCCTCCA
    ACTCCTTCAA AAAGTTACGT GAACAGCCAC TTTATCACCG CAGAAGAATG CCTGAAGCTT
    TTCCCTAGGA GGCTGAAGGA GTCATTCCGG GACCCCTACT CTGCCTTCTT TAAAACAGAT
    GCTGACAGGG ATGGCATAAT CAACATGCAT GACCTTCACA GACTGCTCCT GCATCTACTG
    CTTAATCTCA AAGACGACGA GTTTGAGCGC TTCCTTGGCC TTCTTGGCTT GAGACTTAGT
    GTCACTTTAA ATTTTCGGGA ATTTCAAAAT TTGTGTGAGA AGAGACCATG GAGAACAGAT
    GAAGCGCCTC AAAGACTCAT TAGACCAAAA CAGAAGGTTG CGGATTCAGA ACTAGCTTGT
    GAGCAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAAACA GATGGTCAGA CTTGTCTAAG
    AATTTTCTAG AAACCGATAA TGAGGGCAAT GGCATTCTTC GACGCCGGGA CATAAAGAAC
    GCACTGTACG GTTTTGATAT TCCCCTCACA CCAAGAGAGT TTGAAAAGCT TTGGGCAAGA
    TACGACACCG AGGGAAAAGG GCACATTACT TACCAGGAAT TTTTACAGAA ATTGGGTATT
    AACTATTCGC CTGCTGTCCA TCGGCCCTGT GCAGAGGATT ATTTCAACTT CATGGGTCAT
    TTTACAAAGC CACAGCAGCT ACAGGAAGAG ATGAAGGAGC TGCAGCAGAG CACAGAGAAG
    GCTGTGGCAG CCAGGGATAA GCTTATGGAC CGCCATCAAG ATATCAGCAA AGCATTCACC
    AAAACTGATC AATCCAAAAC CAACTACATA TCCATATGCA AGATGCAGGA AGTGCTGGAA
    GAATGTGGAT GTTCTCTTAC CGAAGGGGAG CTGACCCATC TGCTAAACAG TTGGGGAGTC
    AGCCGGCATG ATAATGCTAT CAATTACCTC GACTTCCTGA GAGCAGTGGA GAACAGCAAG
    TCAACAGGAG CTCAGCCCAA GGAAAAAGAA GAGAGCATGC CAATCAATTT TGCAACGCTG
    AATCCACAGG AGGCTGTGAG GAAGATCCAG GAAGTAGTTG AGTCCTCCCA GCTGGCTTTG
    TCCACGGCAT TTTCTGCATT GGATAAAGAG GATACAGGAT TTGTAAAGGC TACAGAATTC
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACGGACA ATCAGTATCA TTATTTTTTG
    AGGAAACTAA GAATTCATCT AACCCCCTAT ATAAATTGGA AATATTTTCT CCAGAACTTT
    AGCTGTTTCC TTGAGGAGAC AGCTGATGAG TGGGCTGAGA AAATGCCCAA AGGCCCGCCG
    CCTACCTCTC CCAAGGCCAC AGCCGACAGA GACATCCTGG CTCGCCTCCA CAAAGCAGTG
    ACTTCCCATT ACCATGCCAT CACCCAGGAG TTTGAGAATT TTGACACCAT GAAAACGAAC
    ACCATCTCCA GAGAGGAGTT TAGGGCCATT TGTAATCGCC GCGTCCAAAT CCTGACGGAC
    GAACAGTTTG ACAGACTCTG GAACGAGATG CCAGTCAATG CCAAGGGGAG GCTGAAATAC
    CCGGACTTCC TGAGCAGGTT CAGTTCCGAG ACAGCAGCCA CACCAATGGC CACTGGTGAC
    TCGGCCGTGG CCCAGAGAGG GAGCAGTGTC CCTGACGTCT CGGAAGGCAC CAGATCTGCC
    CTCTCATTGC CCACTCAGGA GCTGAGACCA GGGTCAAAGT CGCAGAGCCA CCCCTGTACT
    CCAGCGAGCA CCACCGTGAT CCCGGGCACT CCACCCTTGC AGAACTGTGA TCCCATAGAG
    AGCAGGCTCC GGAAGAGAAT CCAGGGCTGC TGGCGCCAGC TCCTGAAAGA ATGCAAGGAG
    AAGGACGTGG CCAGACAGGG GGACATCAAC GCCTCCGATT TCCTGGCTCT TGTGGAGAAA
    TTCAACCTGG ACATAAGCAA AGAGGAGTGT CAGCAGCTCA TTATAAAATA CGACTTAAAG
    AGCAACGGGA AATTTGCATA CTGTGACTTC ATTCAGAGCT GTGTCCTCCT GCTAAAAGCA
    AAGGAAAGCT CACTGATGCA CAGGATGAAG ATCCAGAATG CACACAAGAT GAAAGAAGCC
    GGCGCGGAGA CGCCATCTTT TTACTCTGCT CTGCTGCGTA TTCAGCCCAA AATTGTGCAC
    TGCTGGAGGC CAATGCGGCG CACGTTCAAA AGCTATGATG AGGCTGGAAC AGGGCTGCTA
    AGCGTCGCAG ATTTCAGGAC GGTGAGCACA GCTCTGCGCC CAGTGCCTCT GCAGGGAGCA
    GCTGCCAAAT GCCAGCGGCC AGAAACTTCC CACGGGGAGC CCTCTACCTG CTGCCGAAGG
    CCCGGTGTGA CCTGGCTGCT TCAGATCTCA CTTCCCTGGG ACCCCCAAGT TATCCAGTCC
    TGGGTCCACT CGCCTCACCT TCAGACCCCG GGTTCTCCTT CCCAAAGGTT GCTGGAGCCC
    TGCGTGGATG CCCCATACCT GCATCGCCAG CTCCTCAGGG TCCTGAGACA GTACAGCATC
    AACCTCTCTG AGGAAGAGTT CTTCCATATT CTGGAGTATT ACGATAAGAC GCTGTCTTCA
    AAAATCTCCT ACAACGACTT CCTCCGGGCA TTCCTCCAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71506
    Clone ID Related Accession (Same CDS sequence) XM_017028912.2 , XM_017028912.1
    Accession Version XM_017028912.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028912.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAGTTTTCA AAAACATTAA GACTGTTATG 
    AAAGCCTTTG AGCTCATTGA TGTTAACAAG ACTGGACTGG TTCGACCGCA GGAGCTAAGA
    AGGGTTCTGG AGACCTTCTG TATGAAGTTA AGAGACGAGG AATACGAAAA GTTTTCGAAA
    CACTACAACA TCCACAAGGA TACTGCAGTA GATTACAACG TGTTTTTGAA GAATCTCAGC
    ATAAATAACG ACTTGAACCT TAGATATTGT ATGGGAAATC AAGAGGTCTC GTTGGAGAAT
    CAACAAGCCA AAAATTCCAA AAAGGAACGT TTGCTAGGTT CTGCATCATC TGAAGATATC
    TGGAGAAACT ACTCCTTGGA TGAAATTGAG AGGAACTTTT GTCTACAACT TTCGAAGTCT
    TATGAAAAGG TTGAAAAGGC CCTCAGTGCA GGGGACCCCT GTAAAGGTGG CTACGTGTCT
    TTTAATTATC TAAAGATTGT CCTCGACACT TTTGTATACC AAATACCAAG AAGAATTTTT
    ATCCAGTTAA TGAAAAGATT TGGACTTAAA GCCACCACTA AAATCAATTG GAAGCAATTT
    CTAACATCAT TTCATGAGCC TCAGGGGTTG CAAGTTAGCA GTAAAGGTCC CCTGACAAAA
    AGAAATAGCA TCAACTCTAG AAATGAATCT CACAAGGAAA ACATCATCAC AAAGTTATTT
    AGACACACTG AAGATCACTC TGCGTCACTG AAGAAAGCAT TACTGATAAT CAACACTAAA
    CCCGATGGAC CGATAACAAG AGAAGAATTT CGATATATTC TAAATTGCAT GGCTGTAAAA
    CTAAGCGATT CAGAATTCAA AGAACTAATG CAAATGCTTG ACCCTGGGGA CACTGGAGTG
    GTCAACACCA GCATGTTTAT TGATCTGATT GAAGAGAACT GTAGGATGAG AAAGACATCT
    CCCTGTACAG ATGCTAAGAC ACCTTTCCTC CTGGCCTGGG ATTCAGTGGA AGAAATTGTT
    CATGATACAA TTACTAGGAA CCTACAAGCT TTTTATAACA TGCTACGCTC ATATGACCTT
    GGAGACACAG GGCGCATTGG CCGAAATAAT TTCAAGAAAA TCATGCACGT CTTCTGTCCA
    TTTTTAACGA ATGCACATTT CATAAAACTC TGCAGTAAGA TTCAGGACAT TGGTTCAGGA
    AGAATCCTTT ACAAGAAACT TTTGGCATGC ATAGGAATTG ATGGCCCACC CACTGTCTCT
    CCAGTTCTTG TTCCAAAGGA TCAGCTGTTA AGTGAACATT TACAAAAAGA TGAACAGCAG
    CAGCCAGATC TTTCTGAGAG AACCAAGCTC ACGGAGGATA AAACCACCCT GACCAAGAAG
    ATGACCACAG AAGAAGTGAT TGAAAAATTC AAAAAGTGTA TACAGCAGCA GGACCCGGCA
    TTCAAAAAAC GATTTCTTGA CTTCAGCAAG GAGCCTAATG GAAAAATTAA CGTGCATGAC
    TTTAAGAAGG TACTGGAAGA CACTGGGATG CCCATGGACG ATGATCAGTA TGCCCTGCTG
    ACCACTAAAA TAGGCTTCGA GAAGGAAGGG ATGAGCTATC TTGATTTTGC AGCAGGATTT
    GAAGATCCTC CAATGAGAGG GCCGGAAACC ACTCCGCCGC AGCCTCCAAC TCCTTCAAAA
    AGTTACGTGA ACAGCCACTT TATCACCGCA GAAGAATGCC TGAAGCTTTT CCCTAGGAGG
    CTGAAGGAGT CATTCCGGGA CCCCTACTCT GCCTTCTTTA AAACAGATGC TGACAGGGAT
    GGCATAATCA ACATGCATGA CCTTCACAGA CTGCTCCTGC ATCTACTGCT TAATCTCAAA
    GACGACGAGT TTGAGCGCTT CCTTGGCCTT CTTGGCTTGA GACTTAGTGT CACTTTAAAT
    TTTCGGGAAT TTCAAAATTT GTGTGAGAAG AGACCATGGA GAACAGATGA AGCGCCTCAA
    AGACTCATTA GACCAAAACA GAAGGTTGCG GATTCAGAAC TAGCTTGTGA GCAGGCTCAT
    CAGTATCTTG TTACCAAAGC AAAAAACAGA TGGTCAGACT TGTCTAAGAA TTTTCTAGAA
    ACCGATAATG AGGGCAATGG CATTCTTCGA CGCCGGGACA TAAAGAACGC ACTGTACGGT
    TTTGATATTC CCCTCACACC AAGAGAGTTT GAAAAGCTTT GGGCAAGATA CGACACCGAG
    GGAAAAGGGC ACATTACTTA CCAGGAATTT TTACAGAAAT TGGGTATTAA CTATTCGCCT
    GCTGTCCATC GGCCCTGTGC AGAGGATTAT TTCAACTTCA TGGGTCATTT TACAAAGCCA
    CAGCAGCTAC AGGAAGAGAT GAAGGAGCTG CAGCAGAGCA CAGAGAAGGC TGTGGCAGCC
    AGGGATAAGC TTATGGACCG CCATCAAGAT ATCAGCAAAG CATTCACCAA AACTGATCAA
    TCCAAAACCA ACTACATATC CATATGCAAG ATGCAGGAAG TGCTGGAAGA ATGTGGATGT
    TCTCTTACCG AAGGGGAGCT GACCCATCTG CTAAACAGTT GGGGAGTCAG CCGGCATGAT
    AATGCTATCA ATTACCTCGA CTTCCTGAGA GCAGTGGAGA ACAGCAAGTC AACAGGAGCT
    CAGCCCAAGG AAAAAGAAGA GAGCATGCCA ATCAATTTTG CAACGCTGAA TCCACAGGAG
    GCTGTGAGGA AGATCCAGGA AGTAGTTGAG TCCTCCCAGC TGGCTTTGTC CACGGCATTT
    TCTGCATTGG ATAAAGAGGA TACAGGATTT GTAAAGGCTA CAGAATTCGG ACAAGTTCTT
    AAGGATTTCT GTTACAAACT AACGGACAAT CAGTATCATT ATTTTTTGAG GAAACTAAGA
    ATTCATCTAA CCCCCTATAT AAATTGGAAA TATTTTCTCC AGAACTTTAG CTGTTTCCTT
    GAGGAGACAG CTGATGAGTG GGCTGAGAAA ATGCCCAAAG GCCCGCCGCC TACCTCTCCC
    AAGGCCACAG CCGACAGAGA CATCCTGGCT CGCCTCCACA AAGCAGTGAC TTCCCATTAC
    CATGCCATCA CCCAGGAGTT TGAGAATTTT GACACCATGA AAACGAACAC CATCTCCAGA
    GAGGAGTTTA GGGCCATTTG TAATCGCCGC GTCCAAATCC TGACGGACGA ACAGTTTGAC
    AGACTCTGGA ACGAGATGCC AGTCAATGCC AAGGGGAGGC TGAAATACCC GGACTTCCTG
    AGCAGGTTCA GTTCCGAGAC AGCAGCCACA CCAATGGCCA CTGGTGACTC GGCCGTGGCC
    CAGAGAGGGA GCAGTGTCCC TGACGTCTCG GAAGGCACCA GATCTGCCCT CTCATTGCCC
    ACTCAGGAGC TGAGACCAGG GTCAAAGTCG CAGAGCCACC CCTGTACTCC AGCGAGCACC
    ACCGTGATCC CGGGCACTCC ACCCTTGCAG AACTGTGATC CCATAGAGAG CAGGCTCCGG
    AAGAGAATCC AGGGCTGCTG GCGCCAGCTC CTGAAAGAAT GCAAGGAGAA GGACGTGGCC
    AGACAGGGGG ACATCAACGC CTCCGATTTC CTGGCTCTTG TGGAGAAATT CAACCTGGAC
    ATAAGCAAAG AGGAGTGTCA GCAGCTCATT ATAAAATACG ACTTAAAGAG CAACGGGAAA
    TTTGCATACT GTGACTTCAT TCAGAGCTGT GTCCTCCTGC TAAAAGCAAA GGAAAGCTCA
    CTGATGCACA GGATGAAGAT CCAGAATGCA CACAAGATGA AAGAAGCCGG CGCGGAGACG
    CCATCTTTTT ACTCTGCTCT GCTGCGTATT CAGCCCAAAA TTGTGCACTG CTGGAGGCCA
    ATGCGGCGCA CGTTCAAAAG CTATGATGAG GCTGGAACAG GGCTGCTAAG CGTCGCAGAT
    TTCAGGACGG TGAGCACAGC TCTGCGCCCA GTGCCTCTGC AGGGAGCAGC TGCCAAATGC
    CAGCGGCCAG AAACTTCCCA CGGGGAGCCC TCTACCTGCT GCCGAAGGCC CGGTGTGACC
    TGGCTGCTTC AGATCTCACT TCCCTGGGAC CCCCAAGTTA TCCAGTCCTG GGTCCACTCG
    CCTCACCTTC AGACCCCGGG TTCTCCTTCC CAAAGGTTGC TGGAGCCCTG CGTGGATGCC
    CCATACCTGC ATCGCCAGCT CCTCAGGGTC CTGAGACAGT ACAGCATCAA CCTCTCTGAG
    GAAGAGTTCT TCCATATTCT GGAGTATTAC GATAAGACGC TGTCTTCAAA AATCTCCTAC
    AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884401.1
    CDS1061..5350
    Translation

    Target ORF information:

    RefSeq Version XM_017028912.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028912.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAGTTTTCA AAAACATTAA GACTGTTATG 
    AAAGCCTTTG AGCTCATTGA TGTTAACAAG ACTGGACTGG TTCGACCGCA GGAGCTAAGA
    AGGGTTCTGG AGACCTTCTG TATGAAGTTA AGAGACGAGG AATACGAAAA GTTTTCGAAA
    CACTACAACA TCCACAAGGA TACTGCAGTA GATTACAACG TGTTTTTGAA GAATCTCAGC
    ATAAATAACG ACTTGAACCT TAGATATTGT ATGGGAAATC AAGAGGTCTC GTTGGAGAAT
    CAACAAGCCA AAAATTCCAA AAAGGAACGT TTGCTAGGTT CTGCATCATC TGAAGATATC
    TGGAGAAACT ACTCCTTGGA TGAAATTGAG AGGAACTTTT GTCTACAACT TTCGAAGTCT
    TATGAAAAGG TTGAAAAGGC CCTCAGTGCA GGGGACCCCT GTAAAGGTGG CTACGTGTCT
    TTTAATTATC TAAAGATTGT CCTCGACACT TTTGTATACC AAATACCAAG AAGAATTTTT
    ATCCAGTTAA TGAAAAGATT TGGACTTAAA GCCACCACTA AAATCAATTG GAAGCAATTT
    CTAACATCAT TTCATGAGCC TCAGGGGTTG CAAGTTAGCA GTAAAGGTCC CCTGACAAAA
    AGAAATAGCA TCAACTCTAG AAATGAATCT CACAAGGAAA ACATCATCAC AAAGTTATTT
    AGACACACTG AAGATCACTC TGCGTCACTG AAGAAAGCAT TACTGATAAT CAACACTAAA
    CCCGATGGAC CGATAACAAG AGAAGAATTT CGATATATTC TAAATTGCAT GGCTGTAAAA
    CTAAGCGATT CAGAATTCAA AGAACTAATG CAAATGCTTG ACCCTGGGGA CACTGGAGTG
    GTCAACACCA GCATGTTTAT TGATCTGATT GAAGAGAACT GTAGGATGAG AAAGACATCT
    CCCTGTACAG ATGCTAAGAC ACCTTTCCTC CTGGCCTGGG ATTCAGTGGA AGAAATTGTT
    CATGATACAA TTACTAGGAA CCTACAAGCT TTTTATAACA TGCTACGCTC ATATGACCTT
    GGAGACACAG GGCGCATTGG CCGAAATAAT TTCAAGAAAA TCATGCACGT CTTCTGTCCA
    TTTTTAACGA ATGCACATTT CATAAAACTC TGCAGTAAGA TTCAGGACAT TGGTTCAGGA
    AGAATCCTTT ACAAGAAACT TTTGGCATGC ATAGGAATTG ATGGCCCACC CACTGTCTCT
    CCAGTTCTTG TTCCAAAGGA TCAGCTGTTA AGTGAACATT TACAAAAAGA TGAACAGCAG
    CAGCCAGATC TTTCTGAGAG AACCAAGCTC ACGGAGGATA AAACCACCCT GACCAAGAAG
    ATGACCACAG AAGAAGTGAT TGAAAAATTC AAAAAGTGTA TACAGCAGCA GGACCCGGCA
    TTCAAAAAAC GATTTCTTGA CTTCAGCAAG GAGCCTAATG GAAAAATTAA CGTGCATGAC
    TTTAAGAAGG TACTGGAAGA CACTGGGATG CCCATGGACG ATGATCAGTA TGCCCTGCTG
    ACCACTAAAA TAGGCTTCGA GAAGGAAGGG ATGAGCTATC TTGATTTTGC AGCAGGATTT
    GAAGATCCTC CAATGAGAGG GCCGGAAACC ACTCCGCCGC AGCCTCCAAC TCCTTCAAAA
    AGTTACGTGA ACAGCCACTT TATCACCGCA GAAGAATGCC TGAAGCTTTT CCCTAGGAGG
    CTGAAGGAGT CATTCCGGGA CCCCTACTCT GCCTTCTTTA AAACAGATGC TGACAGGGAT
    GGCATAATCA ACATGCATGA CCTTCACAGA CTGCTCCTGC ATCTACTGCT TAATCTCAAA
    GACGACGAGT TTGAGCGCTT CCTTGGCCTT CTTGGCTTGA GACTTAGTGT CACTTTAAAT
    TTTCGGGAAT TTCAAAATTT GTGTGAGAAG AGACCATGGA GAACAGATGA AGCGCCTCAA
    AGACTCATTA GACCAAAACA GAAGGTTGCG GATTCAGAAC TAGCTTGTGA GCAGGCTCAT
    CAGTATCTTG TTACCAAAGC AAAAAACAGA TGGTCAGACT TGTCTAAGAA TTTTCTAGAA
    ACCGATAATG AGGGCAATGG CATTCTTCGA CGCCGGGACA TAAAGAACGC ACTGTACGGT
    TTTGATATTC CCCTCACACC AAGAGAGTTT GAAAAGCTTT GGGCAAGATA CGACACCGAG
    GGAAAAGGGC ACATTACTTA CCAGGAATTT TTACAGAAAT TGGGTATTAA CTATTCGCCT
    GCTGTCCATC GGCCCTGTGC AGAGGATTAT TTCAACTTCA TGGGTCATTT TACAAAGCCA
    CAGCAGCTAC AGGAAGAGAT GAAGGAGCTG CAGCAGAGCA CAGAGAAGGC TGTGGCAGCC
    AGGGATAAGC TTATGGACCG CCATCAAGAT ATCAGCAAAG CATTCACCAA AACTGATCAA
    TCCAAAACCA ACTACATATC CATATGCAAG ATGCAGGAAG TGCTGGAAGA ATGTGGATGT
    TCTCTTACCG AAGGGGAGCT GACCCATCTG CTAAACAGTT GGGGAGTCAG CCGGCATGAT
    AATGCTATCA ATTACCTCGA CTTCCTGAGA GCAGTGGAGA ACAGCAAGTC AACAGGAGCT
    CAGCCCAAGG AAAAAGAAGA GAGCATGCCA ATCAATTTTG CAACGCTGAA TCCACAGGAG
    GCTGTGAGGA AGATCCAGGA AGTAGTTGAG TCCTCCCAGC TGGCTTTGTC CACGGCATTT
    TCTGCATTGG ATAAAGAGGA TACAGGATTT GTAAAGGCTA CAGAATTCGG ACAAGTTCTT
    AAGGATTTCT GTTACAAACT AACGGACAAT CAGTATCATT ATTTTTTGAG GAAACTAAGA
    ATTCATCTAA CCCCCTATAT AAATTGGAAA TATTTTCTCC AGAACTTTAG CTGTTTCCTT
    GAGGAGACAG CTGATGAGTG GGCTGAGAAA ATGCCCAAAG GCCCGCCGCC TACCTCTCCC
    AAGGCCACAG CCGACAGAGA CATCCTGGCT CGCCTCCACA AAGCAGTGAC TTCCCATTAC
    CATGCCATCA CCCAGGAGTT TGAGAATTTT GACACCATGA AAACGAACAC CATCTCCAGA
    GAGGAGTTTA GGGCCATTTG TAATCGCCGC GTCCAAATCC TGACGGACGA ACAGTTTGAC
    AGACTCTGGA ACGAGATGCC AGTCAATGCC AAGGGGAGGC TGAAATACCC GGACTTCCTG
    AGCAGGTTCA GTTCCGAGAC AGCAGCCACA CCAATGGCCA CTGGTGACTC GGCCGTGGCC
    CAGAGAGGGA GCAGTGTCCC TGACGTCTCG GAAGGCACCA GATCTGCCCT CTCATTGCCC
    ACTCAGGAGC TGAGACCAGG GTCAAAGTCG CAGAGCCACC CCTGTACTCC AGCGAGCACC
    ACCGTGATCC CGGGCACTCC ACCCTTGCAG AACTGTGATC CCATAGAGAG CAGGCTCCGG
    AAGAGAATCC AGGGCTGCTG GCGCCAGCTC CTGAAAGAAT GCAAGGAGAA GGACGTGGCC
    AGACAGGGGG ACATCAACGC CTCCGATTTC CTGGCTCTTG TGGAGAAATT CAACCTGGAC
    ATAAGCAAAG AGGAGTGTCA GCAGCTCATT ATAAAATACG ACTTAAAGAG CAACGGGAAA
    TTTGCATACT GTGACTTCAT TCAGAGCTGT GTCCTCCTGC TAAAAGCAAA GGAAAGCTCA
    CTGATGCACA GGATGAAGAT CCAGAATGCA CACAAGATGA AAGAAGCCGG CGCGGAGACG
    CCATCTTTTT ACTCTGCTCT GCTGCGTATT CAGCCCAAAA TTGTGCACTG CTGGAGGCCA
    ATGCGGCGCA CGTTCAAAAG CTATGATGAG GCTGGAACAG GGCTGCTAAG CGTCGCAGAT
    TTCAGGACGG TGAGCACAGC TCTGCGCCCA GTGCCTCTGC AGGGAGCAGC TGCCAAATGC
    CAGCGGCCAG AAACTTCCCA CGGGGAGCCC TCTACCTGCT GCCGAAGGCC CGGTGTGACC
    TGGCTGCTTC AGATCTCACT TCCCTGGGAC CCCCAAGTTA TCCAGTCCTG GGTCCACTCG
    CCTCACCTTC AGACCCCGGG TTCTCCTTCC CAAAGGTTGC TGGAGCCCTG CGTGGATGCC
    CCATACCTGC ATCGCCAGCT CCTCAGGGTC CTGAGACAGT ACAGCATCAA CCTCTCTGAG
    GAAGAGTTCT TCCATATTCT GGAGTATTAC GATAAGACGC TGTCTTCAAA AATCTCCTAC
    AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71506
    Clone ID Related Accession (Same CDS sequence) XM_017028912.2 , XM_017028912.1
    Accession Version XM_017028912.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAGTTTTCA AAAACATTAA GACTGTTATG 
    AAAGCCTTTG AGCTCATTGA TGTTAACAAG ACTGGACTGG TTCGACCGCA GGAGCTAAGA
    AGGGTTCTGG AGACCTTCTG TATGAAGTTA AGAGACGAGG AATACGAAAA GTTTTCGAAA
    CACTACAACA TCCACAAGGA TACTGCAGTA GATTACAACG TGTTTTTGAA GAATCTCAGC
    ATAAATAACG ACTTGAACCT TAGATATTGT ATGGGAAATC AAGAGGTCTC GTTGGAGAAT
    CAACAAGCCA AAAATTCCAA AAAGGAACGT TTGCTAGGTT CTGCATCATC TGAAGATATC
    TGGAGAAACT ACTCCTTGGA TGAAATTGAG AGGAACTTTT GTCTACAACT TTCGAAGTCT
    TATGAAAAGG TTGAAAAGGC CCTCAGTGCA GGGGACCCCT GTAAAGGTGG CTACGTGTCT
    TTTAATTATC TAAAGATTGT CCTCGACACT TTTGTATACC AAATACCAAG AAGAATTTTT
    ATCCAGTTAA TGAAAAGATT TGGACTTAAA GCCACCACTA AAATCAATTG GAAGCAATTT
    CTAACATCAT TTCATGAGCC TCAGGGGTTG CAAGTTAGCA GTAAAGGTCC CCTGACAAAA
    AGAAATAGCA TCAACTCTAG AAATGAATCT CACAAGGAAA ACATCATCAC AAAGTTATTT
    AGACACACTG AAGATCACTC TGCGTCACTG AAGAAAGCAT TACTGATAAT CAACACTAAA
    CCCGATGGAC CGATAACAAG AGAAGAATTT CGATATATTC TAAATTGCAT GGCTGTAAAA
    CTAAGCGATT CAGAATTCAA AGAACTAATG CAAATGCTTG ACCCTGGGGA CACTGGAGTG
    GTCAACACCA GCATGTTTAT TGATCTGATT GAAGAGAACT GTAGGATGAG AAAGACATCT
    CCCTGTACAG ATGCTAAGAC ACCTTTCCTC CTGGCCTGGG ATTCAGTGGA AGAAATTGTT
    CATGATACAA TTACTAGGAA CCTACAAGCT TTTTATAACA TGCTACGCTC ATATGACCTT
    GGAGACACAG GGCGCATTGG CCGAAATAAT TTCAAGAAAA TCATGCACGT CTTCTGTCCA
    TTTTTAACGA ATGCACATTT CATAAAACTC TGCAGTAAGA TTCAGGACAT TGGTTCAGGA
    AGAATCCTTT ACAAGAAACT TTTGGCATGC ATAGGAATTG ATGGCCCACC CACTGTCTCT
    CCAGTTCTTG TTCCAAAGGA TCAGCTGTTA AGTGAACATT TACAAAAAGA TGAACAGCAG
    CAGCCAGATC TTTCTGAGAG AACCAAGCTC ACGGAGGATA AAACCACCCT GACCAAGAAG
    ATGACCACAG AAGAAGTGAT TGAAAAATTC AAAAAGTGTA TACAGCAGCA GGACCCGGCA
    TTCAAAAAAC GATTTCTTGA CTTCAGCAAG GAGCCTAATG GAAAAATTAA CGTGCATGAC
    TTTAAGAAGG TACTGGAAGA CACTGGGATG CCCATGGACG ATGATCAGTA TGCCCTGCTG
    ACCACTAAAA TAGGCTTCGA GAAGGAAGGG ATGAGCTATC TTGATTTTGC AGCAGGATTT
    GAAGATCCTC CAATGAGAGG GCCGGAAACC ACTCCGCCGC AGCCTCCAAC TCCTTCAAAA
    AGTTACGTGA ACAGCCACTT TATCACCGCA GAAGAATGCC TGAAGCTTTT CCCTAGGAGG
    CTGAAGGAGT CATTCCGGGA CCCCTACTCT GCCTTCTTTA AAACAGATGC TGACAGGGAT
    GGCATAATCA ACATGCATGA CCTTCACAGA CTGCTCCTGC ATCTACTGCT TAATCTCAAA
    GACGACGAGT TTGAGCGCTT CCTTGGCCTT CTTGGCTTGA GACTTAGTGT CACTTTAAAT
    TTTCGGGAAT TTCAAAATTT GTGTGAGAAG AGACCATGGA GAACAGATGA AGCGCCTCAA
    AGACTCATTA GACCAAAACA GAAGGTTGCG GATTCAGAAC TAGCTTGTGA GCAGGCTCAT
    CAGTATCTTG TTACCAAAGC AAAAAACAGA TGGTCAGACT TGTCTAAGAA TTTTCTAGAA
    ACCGATAATG AGGGCAATGG CATTCTTCGA CGCCGGGACA TAAAGAACGC ACTGTACGGT
    TTTGATATTC CCCTCACACC AAGAGAGTTT GAAAAGCTTT GGGCAAGATA CGACACCGAG
    GGAAAAGGGC ACATTACTTA CCAGGAATTT TTACAGAAAT TGGGTATTAA CTATTCGCCT
    GCTGTCCATC GGCCCTGTGC AGAGGATTAT TTCAACTTCA TGGGTCATTT TACAAAGCCA
    CAGCAGCTAC AGGAAGAGAT GAAGGAGCTG CAGCAGAGCA CAGAGAAGGC TGTGGCAGCC
    AGGGATAAGC TTATGGACCG CCATCAAGAT ATCAGCAAAG CATTCACCAA AACTGATCAA
    TCCAAAACCA ACTACATATC CATATGCAAG ATGCAGGAAG TGCTGGAAGA ATGTGGATGT
    TCTCTTACCG AAGGGGAGCT GACCCATCTG CTAAACAGTT GGGGAGTCAG CCGGCATGAT
    AATGCTATCA ATTACCTCGA CTTCCTGAGA GCAGTGGAGA ACAGCAAGTC AACAGGAGCT
    CAGCCCAAGG AAAAAGAAGA GAGCATGCCA ATCAATTTTG CAACGCTGAA TCCACAGGAG
    GCTGTGAGGA AGATCCAGGA AGTAGTTGAG TCCTCCCAGC TGGCTTTGTC CACGGCATTT
    TCTGCATTGG ATAAAGAGGA TACAGGATTT GTAAAGGCTA CAGAATTCGG ACAAGTTCTT
    AAGGATTTCT GTTACAAACT AACGGACAAT CAGTATCATT ATTTTTTGAG GAAACTAAGA
    ATTCATCTAA CCCCCTATAT AAATTGGAAA TATTTTCTCC AGAACTTTAG CTGTTTCCTT
    GAGGAGACAG CTGATGAGTG GGCTGAGAAA ATGCCCAAAG GCCCGCCGCC TACCTCTCCC
    AAGGCCACAG CCGACAGAGA CATCCTGGCT CGCCTCCACA AAGCAGTGAC TTCCCATTAC
    CATGCCATCA CCCAGGAGTT TGAGAATTTT GACACCATGA AAACGAACAC CATCTCCAGA
    GAGGAGTTTA GGGCCATTTG TAATCGCCGC GTCCAAATCC TGACGGACGA ACAGTTTGAC
    AGACTCTGGA ACGAGATGCC AGTCAATGCC AAGGGGAGGC TGAAATACCC GGACTTCCTG
    AGCAGGTTCA GTTCCGAGAC AGCAGCCACA CCAATGGCCA CTGGTGACTC GGCCGTGGCC
    CAGAGAGGGA GCAGTGTCCC TGACGTCTCG GAAGGCACCA GATCTGCCCT CTCATTGCCC
    ACTCAGGAGC TGAGACCAGG GTCAAAGTCG CAGAGCCACC CCTGTACTCC AGCGAGCACC
    ACCGTGATCC CGGGCACTCC ACCCTTGCAG AACTGTGATC CCATAGAGAG CAGGCTCCGG
    AAGAGAATCC AGGGCTGCTG GCGCCAGCTC CTGAAAGAAT GCAAGGAGAA GGACGTGGCC
    AGACAGGGGG ACATCAACGC CTCCGATTTC CTGGCTCTTG TGGAGAAATT CAACCTGGAC
    ATAAGCAAAG AGGAGTGTCA GCAGCTCATT ATAAAATACG ACTTAAAGAG CAACGGGAAA
    TTTGCATACT GTGACTTCAT TCAGAGCTGT GTCCTCCTGC TAAAAGCAAA GGAAAGCTCA
    CTGATGCACA GGATGAAGAT CCAGAATGCA CACAAGATGA AAGAAGCCGG CGCGGAGACG
    CCATCTTTTT ACTCTGCTCT GCTGCGTATT CAGCCCAAAA TTGTGCACTG CTGGAGGCCA
    ATGCGGCGCA CGTTCAAAAG CTATGATGAG GCTGGAACAG GGCTGCTAAG CGTCGCAGAT
    TTCAGGACGG TGAGCACAGC TCTGCGCCCA GTGCCTCTGC AGGGAGCAGC TGCCAAATGC
    CAGCGGCCAG AAACTTCCCA CGGGGAGCCC TCTACCTGCT GCCGAAGGCC CGGTGTGACC
    TGGCTGCTTC AGATCTCACT TCCCTGGGAC CCCCAAGTTA TCCAGTCCTG GGTCCACTCG
    CCTCACCTTC AGACCCCGGG TTCTCCTTCC CAAAGGTTGC TGGAGCCCTG CGTGGATGCC
    CCATACCTGC ATCGCCAGCT CCTCAGGGTC CTGAGACAGT ACAGCATCAA CCTCTCTGAG
    GAAGAGTTCT TCCATATTCT GGAGTATTAC GATAAGACGC TGTCTTCAAA AATCTCCTAC
    AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884401.1
    CDS487..4776
    Translation

    Target ORF information:

    RefSeq Version XM_017028912.1
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028912.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGTTCCCC AAATAAGTTC AGATCTTCTT CAAGTTTTCA AAAACATTAA GACTGTTATG 
    AAAGCCTTTG AGCTCATTGA TGTTAACAAG ACTGGACTGG TTCGACCGCA GGAGCTAAGA
    AGGGTTCTGG AGACCTTCTG TATGAAGTTA AGAGACGAGG AATACGAAAA GTTTTCGAAA
    CACTACAACA TCCACAAGGA TACTGCAGTA GATTACAACG TGTTTTTGAA GAATCTCAGC
    ATAAATAACG ACTTGAACCT TAGATATTGT ATGGGAAATC AAGAGGTCTC GTTGGAGAAT
    CAACAAGCCA AAAATTCCAA AAAGGAACGT TTGCTAGGTT CTGCATCATC TGAAGATATC
    TGGAGAAACT ACTCCTTGGA TGAAATTGAG AGGAACTTTT GTCTACAACT TTCGAAGTCT
    TATGAAAAGG TTGAAAAGGC CCTCAGTGCA GGGGACCCCT GTAAAGGTGG CTACGTGTCT
    TTTAATTATC TAAAGATTGT CCTCGACACT TTTGTATACC AAATACCAAG AAGAATTTTT
    ATCCAGTTAA TGAAAAGATT TGGACTTAAA GCCACCACTA AAATCAATTG GAAGCAATTT
    CTAACATCAT TTCATGAGCC TCAGGGGTTG CAAGTTAGCA GTAAAGGTCC CCTGACAAAA
    AGAAATAGCA TCAACTCTAG AAATGAATCT CACAAGGAAA ACATCATCAC AAAGTTATTT
    AGACACACTG AAGATCACTC TGCGTCACTG AAGAAAGCAT TACTGATAAT CAACACTAAA
    CCCGATGGAC CGATAACAAG AGAAGAATTT CGATATATTC TAAATTGCAT GGCTGTAAAA
    CTAAGCGATT CAGAATTCAA AGAACTAATG CAAATGCTTG ACCCTGGGGA CACTGGAGTG
    GTCAACACCA GCATGTTTAT TGATCTGATT GAAGAGAACT GTAGGATGAG AAAGACATCT
    CCCTGTACAG ATGCTAAGAC ACCTTTCCTC CTGGCCTGGG ATTCAGTGGA AGAAATTGTT
    CATGATACAA TTACTAGGAA CCTACAAGCT TTTTATAACA TGCTACGCTC ATATGACCTT
    GGAGACACAG GGCGCATTGG CCGAAATAAT TTCAAGAAAA TCATGCACGT CTTCTGTCCA
    TTTTTAACGA ATGCACATTT CATAAAACTC TGCAGTAAGA TTCAGGACAT TGGTTCAGGA
    AGAATCCTTT ACAAGAAACT TTTGGCATGC ATAGGAATTG ATGGCCCACC CACTGTCTCT
    CCAGTTCTTG TTCCAAAGGA TCAGCTGTTA AGTGAACATT TACAAAAAGA TGAACAGCAG
    CAGCCAGATC TTTCTGAGAG AACCAAGCTC ACGGAGGATA AAACCACCCT GACCAAGAAG
    ATGACCACAG AAGAAGTGAT TGAAAAATTC AAAAAGTGTA TACAGCAGCA GGACCCGGCA
    TTCAAAAAAC GATTTCTTGA CTTCAGCAAG GAGCCTAATG GAAAAATTAA CGTGCATGAC
    TTTAAGAAGG TACTGGAAGA CACTGGGATG CCCATGGACG ATGATCAGTA TGCCCTGCTG
    ACCACTAAAA TAGGCTTCGA GAAGGAAGGG ATGAGCTATC TTGATTTTGC AGCAGGATTT
    GAAGATCCTC CAATGAGAGG GCCGGAAACC ACTCCGCCGC AGCCTCCAAC TCCTTCAAAA
    AGTTACGTGA ACAGCCACTT TATCACCGCA GAAGAATGCC TGAAGCTTTT CCCTAGGAGG
    CTGAAGGAGT CATTCCGGGA CCCCTACTCT GCCTTCTTTA AAACAGATGC TGACAGGGAT
    GGCATAATCA ACATGCATGA CCTTCACAGA CTGCTCCTGC ATCTACTGCT TAATCTCAAA
    GACGACGAGT TTGAGCGCTT CCTTGGCCTT CTTGGCTTGA GACTTAGTGT CACTTTAAAT
    TTTCGGGAAT TTCAAAATTT GTGTGAGAAG AGACCATGGA GAACAGATGA AGCGCCTCAA
    AGACTCATTA GACCAAAACA GAAGGTTGCG GATTCAGAAC TAGCTTGTGA GCAGGCTCAT
    CAGTATCTTG TTACCAAAGC AAAAAACAGA TGGTCAGACT TGTCTAAGAA TTTTCTAGAA
    ACCGATAATG AGGGCAATGG CATTCTTCGA CGCCGGGACA TAAAGAACGC ACTGTACGGT
    TTTGATATTC CCCTCACACC AAGAGAGTTT GAAAAGCTTT GGGCAAGATA CGACACCGAG
    GGAAAAGGGC ACATTACTTA CCAGGAATTT TTACAGAAAT TGGGTATTAA CTATTCGCCT
    GCTGTCCATC GGCCCTGTGC AGAGGATTAT TTCAACTTCA TGGGTCATTT TACAAAGCCA
    CAGCAGCTAC AGGAAGAGAT GAAGGAGCTG CAGCAGAGCA CAGAGAAGGC TGTGGCAGCC
    AGGGATAAGC TTATGGACCG CCATCAAGAT ATCAGCAAAG CATTCACCAA AACTGATCAA
    TCCAAAACCA ACTACATATC CATATGCAAG ATGCAGGAAG TGCTGGAAGA ATGTGGATGT
    TCTCTTACCG AAGGGGAGCT GACCCATCTG CTAAACAGTT GGGGAGTCAG CCGGCATGAT
    AATGCTATCA ATTACCTCGA CTTCCTGAGA GCAGTGGAGA ACAGCAAGTC AACAGGAGCT
    CAGCCCAAGG AAAAAGAAGA GAGCATGCCA ATCAATTTTG CAACGCTGAA TCCACAGGAG
    GCTGTGAGGA AGATCCAGGA AGTAGTTGAG TCCTCCCAGC TGGCTTTGTC CACGGCATTT
    TCTGCATTGG ATAAAGAGGA TACAGGATTT GTAAAGGCTA CAGAATTCGG ACAAGTTCTT
    AAGGATTTCT GTTACAAACT AACGGACAAT CAGTATCATT ATTTTTTGAG GAAACTAAGA
    ATTCATCTAA CCCCCTATAT AAATTGGAAA TATTTTCTCC AGAACTTTAG CTGTTTCCTT
    GAGGAGACAG CTGATGAGTG GGCTGAGAAA ATGCCCAAAG GCCCGCCGCC TACCTCTCCC
    AAGGCCACAG CCGACAGAGA CATCCTGGCT CGCCTCCACA AAGCAGTGAC TTCCCATTAC
    CATGCCATCA CCCAGGAGTT TGAGAATTTT GACACCATGA AAACGAACAC CATCTCCAGA
    GAGGAGTTTA GGGCCATTTG TAATCGCCGC GTCCAAATCC TGACGGACGA ACAGTTTGAC
    AGACTCTGGA ACGAGATGCC AGTCAATGCC AAGGGGAGGC TGAAATACCC GGACTTCCTG
    AGCAGGTTCA GTTCCGAGAC AGCAGCCACA CCAATGGCCA CTGGTGACTC GGCCGTGGCC
    CAGAGAGGGA GCAGTGTCCC TGACGTCTCG GAAGGCACCA GATCTGCCCT CTCATTGCCC
    ACTCAGGAGC TGAGACCAGG GTCAAAGTCG CAGAGCCACC CCTGTACTCC AGCGAGCACC
    ACCGTGATCC CGGGCACTCC ACCCTTGCAG AACTGTGATC CCATAGAGAG CAGGCTCCGG
    AAGAGAATCC AGGGCTGCTG GCGCCAGCTC CTGAAAGAAT GCAAGGAGAA GGACGTGGCC
    AGACAGGGGG ACATCAACGC CTCCGATTTC CTGGCTCTTG TGGAGAAATT CAACCTGGAC
    ATAAGCAAAG AGGAGTGTCA GCAGCTCATT ATAAAATACG ACTTAAAGAG CAACGGGAAA
    TTTGCATACT GTGACTTCAT TCAGAGCTGT GTCCTCCTGC TAAAAGCAAA GGAAAGCTCA
    CTGATGCACA GGATGAAGAT CCAGAATGCA CACAAGATGA AAGAAGCCGG CGCGGAGACG
    CCATCTTTTT ACTCTGCTCT GCTGCGTATT CAGCCCAAAA TTGTGCACTG CTGGAGGCCA
    ATGCGGCGCA CGTTCAAAAG CTATGATGAG GCTGGAACAG GGCTGCTAAG CGTCGCAGAT
    TTCAGGACGG TGAGCACAGC TCTGCGCCCA GTGCCTCTGC AGGGAGCAGC TGCCAAATGC
    CAGCGGCCAG AAACTTCCCA CGGGGAGCCC TCTACCTGCT GCCGAAGGCC CGGTGTGACC
    TGGCTGCTTC AGATCTCACT TCCCTGGGAC CCCCAAGTTA TCCAGTCCTG GGTCCACTCG
    CCTCACCTTC AGACCCCGGG TTCTCCTTCC CAAAGGTTGC TGGAGCCCTG CGTGGATGCC
    CCATACCTGC ATCGCCAGCT CCTCAGGGTC CTGAGACAGT ACAGCATCAA CCTCTCTGAG
    GAAGAGTTCT TCCATATTCT GGAGTATTAC GATAAGACGC TGTCTTCAAA AATCTCCTAC
    AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu71507
    Clone ID Related Accession (Same CDS sequence) XM_011530325.2
    Accession Version XM_011530325.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4020bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGAAATC AAGAGGTCTC GTTGGAGAAT CAACAAGCCA AAAATTCCAA AAAGGAACGT 
    TTGCTAGGTT CTGCATCATC TGAAGATATC TGGAGAAACT ACTCCTTGGA TGAAATTGAG
    AGGAACTTTT GTCTACAACT TTCGAAGTCT TATGAAAAGG TTGAAAAGGC CCTCAGTGCA
    GGGGACCCCT GTAAAGGTGG CTACGTGTCT TTTAATTATC TAAAGATTGT CCTCGACACT
    TTTGTATACC AAATACCAAG AAGAATTTTT ATCCAGTTAA TGAAAAGATT TGGACTTAAA
    GCCACCACTA AAATCAATTG GAAGCAATTT CTAACATCAT TTCATGAGCC TCAGGGGTTG
    CAAGTTAGCA GTAAAGGTCC CCTGACAAAA AGAAATAGCA TCAACTCTAG AAATGAATCT
    CACAAGGAAA ACATCATCAC AAAGTTATTT AGACACACTG AAGATCACTC TGCGTCACTG
    AAGAAAGCAT TACTGATAAT CAACACTAAA CCCGATGGAC CGATAACAAG AGAAGAATTT
    CGATATATTC TAAATTGCAT GGCTGTAAAA CTAAGCGATT CAGAATTCAA AGAACTAATG
    CAAATGCTTG ACCCTGGGGA CACTGGAGTG GTCAACACCA GCATGTTTAT TGATCTGATT
    GAAGAGAACT GTAGGATGAG AAAGACATCT CCCTGTACAG ATGCTAAGAC ACCTTTCCTC
    CTGGCCTGGG ATTCAGTGGA AGAAATTGTT CATGATACAA TTACTAGGAA CCTACAAGCT
    TTTTATAACA TGCTACGCTC ATATGACCTT GGAGACACAG GGCGCATTGG CCGAAATAAT
    TTCAAGAAAA TCATGCACGT CTTCTGTCCA TTTTTAACGA ATGCACATTT CATAAAACTC
    TGCAGTAAGA TTCAGGACAT TGGTTCAGGA AGAATCCTTT ACAAGAAACT TTTGGCATGC
    ATAGGAATTG ATGGCCCACC CACTGTCTCT CCAGTTCTTG TTCCAAAGGA TCAGCTGTTA
    AGTGAACATT TACAAAAAGA TGAACAGCAG CAGCCAGATC TTTCTGAGAG AACCAAGCTC
    ACGGAGGATA AAACCACCCT GACCAAGAAG ATGACCACAG AAGAAGTGAT TGAAAAATTC
    AAAAAGTGTA TACAGCAGCA GGACCCGGCA TTCAAAAAAC GATTTCTTGA CTTCAGCAAG
    GAGCCTAATG GAAAAATTAA CGTGCATGAC TTTAAGAAGG TACTGGAAGA CACTGGGATG
    CCCATGGACG ATGATCAGTA TGCCCTGCTG ACCACTAAAA TAGGCTTCGA GAAGGAAGGG
    ATGAGCTATC TTGATTTTGC AGCAGGATTT GAAGATCCTC CAATGAGAGG GCCGGAAACC
    ACTCCGCCGC AGCCTCCAAC TCCTTCAAAA AGTTACGTGA ACAGCCACTT TATCACCGCA
    GAAGAATGCC TGAAGCTTTT CCCTAGGAGG CTGAAGGAGT CATTCCGGGA CCCCTACTCT
    GCCTTCTTTA AAACAGATGC TGACAGGGAT GGCATAATCA ACATGCATGA CCTTCACAGA
    CTGCTCCTGC ATCTACTGCT TAATCTCAAA GACGACGAGT TTGAGCGCTT CCTTGGCCTT
    CTTGGCTTGA GACTTAGTGT CACTTTAAAT TTTCGGGAAT TTCAAAATTT GTGTGAGAAG
    AGACCATGGA GAACAGATGA AGCGCCTCAA AGACTCATTA GACCAAAACA GAAGGTTGCG
    GATTCAGAAC TAGCTTGTGA GCAGGCTCAT CAGTATCTTG TTACCAAAGC AAAAAACAGA
    TGGTCAGACT TGTCTAAGAA TTTTCTAGAA ACCGATAATG AGGGCAATGG CATTCTTCGA
    CGCCGGGACA TAAAGAACGC ACTGTACGGT TTTGATATTC CCCTCACACC AAGAGAGTTT
    GAAAAGCTTT GGGCAAGATA CGACACCGAG GGAAAAGGGC ACATTACTTA CCAGGAATTT
    TTACAGAAAT TGGGTATTAA CTATTCGCCT GCTGTCCATC GGCCCTGTGC AGAGGATTAT
    TTCAACTTCA TGGGTCATTT TACAAAGCCA CAGCAGCTAC AGGAAGAGAT GAAGGAGCTG
    CAGCAGAGCA CAGAGAAGGC TGTGGCAGCC AGGGATAAGC TTATGGACCG CCATCAAGAT
    ATCAGCAAAG CATTCACCAA AACTGATCAA TCCAAAACCA ACTACATATC CATATGCAAG
    ATGCAGGAAG TGCTGGAAGA ATGTGGATGT TCTCTTACCG AAGGGGAGCT GACCCATCTG
    CTAAACAGTT GGGGAGTCAG CCGGCATGAT AATGCTATCA ATTACCTCGA CTTCCTGAGA
    GCAGTGGAGA ACAGCAAGTC AACAGGAGCT CAGCCCAAGG AAAAAGAAGA GAGCATGCCA
    ATCAATTTTG CAACGCTGAA TCCACAGGAG GCTGTGAGGA AGATCCAGGA AGTAGTTGAG
    TCCTCCCAGC TGGCTTTGTC CACGGCATTT TCTGCATTGG ATAAAGAGGA TACAGGATTT
    GTAAAGGCTA CAGAATTCGG ACAAGTTCTT AAGGATTTCT GTTACAAACT AACGGACAAT
    CAGTATCATT ATTTTTTGAG GAAACTAAGA ATTCATCTAA CCCCCTATAT AAATTGGAAA
    TATTTTCTCC AGAACTTTAG CTGTTTCCTT GAGGAGACAG CTGATGAGTG GGCTGAGAAA
    ATGCCCAAAG GCCCGCCGCC TACCTCTCCC AAGGCCACAG CCGACAGAGA CATCCTGGCT
    CGCCTCCACA AAGCAGTGAC TTCCCATTAC CATGCCATCA CCCAGGAGTT TGAGAATTTT
    GACACCATGA AAACGAACAC CATCTCCAGA GAGGAGTTTA GGGCCATTTG TAATCGCCGC
    GTCCAAATCC TGACGGACGA ACAGTTTGAC AGACTCTGGA ACGAGATGCC AGTCAATGCC
    AAGGGGAGGC TGAAATACCC GGACTTCCTG AGCAGGTTCA GTTCCGAGAC AGCAGCCACA
    CCAATGGCCA CTGGTGACTC GGCCGTGGCC CAGAGAGGGA GCAGTGTCCC TGACGTCTCG
    GAAGGCACCA GATCTGCCCT CTCATTGCCC ACTCAGGAGC TGAGACCAGG GTCAAAGTCG
    CAGAGCCACC CCTGTACTCC AGCGAGCACC ACCGTGATCC CGGGCACTCC ACCCTTGCAG
    AACTGTGATC CCATAGAGAG CAGGCTCCGG AAGAGAATCC AGGGCTGCTG GCGCCAGCTC
    CTGAAAGAAT GCAAGGAGAA GGACGTGGCC AGACAGGGGG ACATCAACGC CTCCGATTTC
    CTGGCTCTTG TGGAGAAATT CAACCTGGAC ATAAGCAAAG AGGAGTGTCA GCAGCTCATT
    ATAAAATACG ACTTAAAGAG CAACGGGAAA TTTGCATACT GTGACTTCAT TCAGAGCTGT
    GTCCTCCTGC TAAAAGCAAA GGAAAGCTCA CTGATGCACA GGATGAAGAT CCAGAATGCA
    CACAAGATGA AAGAAGCCGG CGCGGAGACG CCATCTTTTT ACTCTGCTCT GCTGCGTATT
    CAGCCCAAAA TTGTGCACTG CTGGAGGCCA ATGCGGCGCA CGTTCAAAAG CTATGATGAG
    GCTGGAACAG GGCTGCTAAG CGTCGCAGAT TTCAGGACGG TGAGCACAGC TCTGCGCCCA
    GTGCCTCTGC AGGGAGCAGC TGCCAAATGC CAGCGGCCAG AAACTTCCCA CGGGGAGCCC
    TCTACCTGCT GCCGAAGGCC CGGTGTGACC TGGCTGCTTC AGATCTCACT TCCCTGGGAC
    CCCCAAGTTA TCCAGTCCTG GGTCCACTCG CCTCACCTTC AGACCCCGGG TTCTCCTTCC
    CAAAGGTTGC TGGAGCCCTG CGTGGATGCC CCATACCTGC ATCGCCAGCT CCTCAGGGTC
    CTGAGACAGT ACAGCATCAA CCTCTCTGAG GAAGAGTTCT TCCATATTCT GGAGTATTAC
    GATAAGACGC TGTCTTCAAA AATCTCCTAC AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528627.1
    CDS160..4179
    Translation

    Target ORF information:

    RefSeq Version XM_011530325.2
    Organism Homo sapiens(human)
    Definition Homo sapiens EF-hand calcium binding domain 6 (EFCAB6), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530325.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGAAATC AAGAGGTCTC GTTGGAGAAT CAACAAGCCA AAAATTCCAA AAAGGAACGT 
    TTGCTAGGTT CTGCATCATC TGAAGATATC TGGAGAAACT ACTCCTTGGA TGAAATTGAG
    AGGAACTTTT GTCTACAACT TTCGAAGTCT TATGAAAAGG TTGAAAAGGC CCTCAGTGCA
    GGGGACCCCT GTAAAGGTGG CTACGTGTCT TTTAATTATC TAAAGATTGT CCTCGACACT
    TTTGTATACC AAATACCAAG AAGAATTTTT ATCCAGTTAA TGAAAAGATT TGGACTTAAA
    GCCACCACTA AAATCAATTG GAAGCAATTT CTAACATCAT TTCATGAGCC TCAGGGGTTG
    CAAGTTAGCA GTAAAGGTCC CCTGACAAAA AGAAATAGCA TCAACTCTAG AAATGAATCT
    CACAAGGAAA ACATCATCAC AAAGTTATTT AGACACACTG AAGATCACTC TGCGTCACTG
    AAGAAAGCAT TACTGATAAT CAACACTAAA CCCGATGGAC CGATAACAAG AGAAGAATTT
    CGATATATTC TAAATTGCAT GGCTGTAAAA CTAAGCGATT CAGAATTCAA AGAACTAATG
    CAAATGCTTG ACCCTGGGGA CACTGGAGTG GTCAACACCA GCATGTTTAT TGATCTGATT
    GAAGAGAACT GTAGGATGAG AAAGACATCT CCCTGTACAG ATGCTAAGAC ACCTTTCCTC
    CTGGCCTGGG ATTCAGTGGA AGAAATTGTT CATGATACAA TTACTAGGAA CCTACAAGCT
    TTTTATAACA TGCTACGCTC ATATGACCTT GGAGACACAG GGCGCATTGG CCGAAATAAT
    TTCAAGAAAA TCATGCACGT CTTCTGTCCA TTTTTAACGA ATGCACATTT CATAAAACTC
    TGCAGTAAGA TTCAGGACAT TGGTTCAGGA AGAATCCTTT ACAAGAAACT TTTGGCATGC
    ATAGGAATTG ATGGCCCACC CACTGTCTCT CCAGTTCTTG TTCCAAAGGA TCAGCTGTTA
    AGTGAACATT TACAAAAAGA TGAACAGCAG CAGCCAGATC TTTCTGAGAG AACCAAGCTC
    ACGGAGGATA AAACCACCCT GACCAAGAAG ATGACCACAG AAGAAGTGAT TGAAAAATTC
    AAAAAGTGTA TACAGCAGCA GGACCCGGCA TTCAAAAAAC GATTTCTTGA CTTCAGCAAG
    GAGCCTAATG GAAAAATTAA CGTGCATGAC TTTAAGAAGG TACTGGAAGA CACTGGGATG
    CCCATGGACG ATGATCAGTA TGCCCTGCTG ACCACTAAAA TAGGCTTCGA GAAGGAAGGG
    ATGAGCTATC TTGATTTTGC AGCAGGATTT GAAGATCCTC CAATGAGAGG GCCGGAAACC
    ACTCCGCCGC AGCCTCCAAC TCCTTCAAAA AGTTACGTGA ACAGCCACTT TATCACCGCA
    GAAGAATGCC TGAAGCTTTT CCCTAGGAGG CTGAAGGAGT CATTCCGGGA CCCCTACTCT
    GCCTTCTTTA AAACAGATGC TGACAGGGAT GGCATAATCA ACATGCATGA CCTTCACAGA
    CTGCTCCTGC ATCTACTGCT TAATCTCAAA GACGACGAGT TTGAGCGCTT CCTTGGCCTT
    CTTGGCTTGA GACTTAGTGT CACTTTAAAT TTTCGGGAAT TTCAAAATTT GTGTGAGAAG
    AGACCATGGA GAACAGATGA AGCGCCTCAA AGACTCATTA GACCAAAACA GAAGGTTGCG
    GATTCAGAAC TAGCTTGTGA GCAGGCTCAT CAGTATCTTG TTACCAAAGC AAAAAACAGA
    TGGTCAGACT TGTCTAAGAA TTTTCTAGAA ACCGATAATG AGGGCAATGG CATTCTTCGA
    CGCCGGGACA TAAAGAACGC ACTGTACGGT TTTGATATTC CCCTCACACC AAGAGAGTTT
    GAAAAGCTTT GGGCAAGATA CGACACCGAG GGAAAAGGGC ACATTACTTA CCAGGAATTT
    TTACAGAAAT TGGGTATTAA CTATTCGCCT GCTGTCCATC GGCCCTGTGC AGAGGATTAT
    TTCAACTTCA TGGGTCATTT TACAAAGCCA CAGCAGCTAC AGGAAGAGAT GAAGGAGCTG
    CAGCAGAGCA CAGAGAAGGC TGTGGCAGCC AGGGATAAGC TTATGGACCG CCATCAAGAT
    ATCAGCAAAG CATTCACCAA AACTGATCAA TCCAAAACCA ACTACATATC CATATGCAAG
    ATGCAGGAAG TGCTGGAAGA ATGTGGATGT TCTCTTACCG AAGGGGAGCT GACCCATCTG
    CTAAACAGTT GGGGAGTCAG CCGGCATGAT AATGCTATCA ATTACCTCGA CTTCCTGAGA
    GCAGTGGAGA ACAGCAAGTC AACAGGAGCT CAGCCCAAGG AAAAAGAAGA GAGCATGCCA
    ATCAATTTTG CAACGCTGAA TCCACAGGAG GCTGTGAGGA AGATCCAGGA AGTAGTTGAG
    TCCTCCCAGC TGGCTTTGTC CACGGCATTT TCTGCATTGG ATAAAGAGGA TACAGGATTT
    GTAAAGGCTA CAGAATTCGG ACAAGTTCTT AAGGATTTCT GTTACAAACT AACGGACAAT
    CAGTATCATT ATTTTTTGAG GAAACTAAGA ATTCATCTAA CCCCCTATAT AAATTGGAAA
    TATTTTCTCC AGAACTTTAG CTGTTTCCTT GAGGAGACAG CTGATGAGTG GGCTGAGAAA
    ATGCCCAAAG GCCCGCCGCC TACCTCTCCC AAGGCCACAG CCGACAGAGA CATCCTGGCT
    CGCCTCCACA AAGCAGTGAC TTCCCATTAC CATGCCATCA CCCAGGAGTT TGAGAATTTT
    GACACCATGA AAACGAACAC CATCTCCAGA GAGGAGTTTA GGGCCATTTG TAATCGCCGC
    GTCCAAATCC TGACGGACGA ACAGTTTGAC AGACTCTGGA ACGAGATGCC AGTCAATGCC
    AAGGGGAGGC TGAAATACCC GGACTTCCTG AGCAGGTTCA GTTCCGAGAC AGCAGCCACA
    CCAATGGCCA CTGGTGACTC GGCCGTGGCC CAGAGAGGGA GCAGTGTCCC TGACGTCTCG
    GAAGGCACCA GATCTGCCCT CTCATTGCCC ACTCAGGAGC TGAGACCAGG GTCAAAGTCG
    CAGAGCCACC CCTGTACTCC AGCGAGCACC ACCGTGATCC CGGGCACTCC ACCCTTGCAG
    AACTGTGATC CCATAGAGAG CAGGCTCCGG AAGAGAATCC AGGGCTGCTG GCGCCAGCTC
    CTGAAAGAAT GCAAGGAGAA GGACGTGGCC AGACAGGGGG ACATCAACGC CTCCGATTTC
    CTGGCTCTTG TGGAGAAATT CAACCTGGAC ATAAGCAAAG AGGAGTGTCA GCAGCTCATT
    ATAAAATACG ACTTAAAGAG CAACGGGAAA TTTGCATACT GTGACTTCAT TCAGAGCTGT
    GTCCTCCTGC TAAAAGCAAA GGAAAGCTCA CTGATGCACA GGATGAAGAT CCAGAATGCA
    CACAAGATGA AAGAAGCCGG CGCGGAGACG CCATCTTTTT ACTCTGCTCT GCTGCGTATT
    CAGCCCAAAA TTGTGCACTG CTGGAGGCCA ATGCGGCGCA CGTTCAAAAG CTATGATGAG
    GCTGGAACAG GGCTGCTAAG CGTCGCAGAT TTCAGGACGG TGAGCACAGC TCTGCGCCCA
    GTGCCTCTGC AGGGAGCAGC TGCCAAATGC CAGCGGCCAG AAACTTCCCA CGGGGAGCCC
    TCTACCTGCT GCCGAAGGCC CGGTGTGACC TGGCTGCTTC AGATCTCACT TCCCTGGGAC
    CCCCAAGTTA TCCAGTCCTG GGTCCACTCG CCTCACCTTC AGACCCCGGG TTCTCCTTCC
    CAAAGGTTGC TGGAGCCCTG CGTGGATGCC CCATACCTGC ATCGCCAGCT CCTCAGGGTC
    CTGAGACAGT ACAGCATCAA CCTCTCTGAG GAAGAGTTCT TCCATATTCT GGAGTATTAC
    GATAAGACGC TGTCTTCAAA AATCTCCTAC AACGACTTCC TCCGGGCATT CCTCCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.