EFCAB6 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_003586161.4	XP_003586209.2	EF-hand calcium-binding domain-containing protein 6 isoform X1
XM_010805781.2	XP_010804083.1	EF-hand calcium-binding domain-containing protein 6 isoform X2
XM_015463631.1	XP_015319117.1	EF-hand calcium-binding domain-containing protein 6 isoform X1
XM_005207460.3	XP_005207517.1	EF-hand calcium-binding domain-containing protein 6 isoform X1
XM_005207462.4	XP_005207519.1	EF-hand calcium-binding domain-containing protein 6 isoform X3
XM_015463632.1	XP_015319118.1	EF-hand calcium-binding domain-containing protein 6 isoform X2
XM_015463633.1	XP_015319119.1	EF-hand calcium-binding domain-containing protein 6 isoform X3
XM_003586161.5	XP_003586209.2	EF-hand calcium-binding domain-containing protein 6 isoform X1
XM_005207460.4	XP_005207517.1	EF-hand calcium-binding domain-containing protein 6 isoform X1
XM_005207462.3	XP_005207519.1	EF-hand calcium-binding domain-containing protein 6 isoform X3
XM_015463630.1	XP_015319116.1	EF-hand calcium-binding domain-containing protein 6 isoform X1
XM_010805781.3	XP_010804083.1	EF-hand calcium-binding domain-containing protein 6 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa20033	XM_015463630.1 Latest version!	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20033	XM_015463631.1 Latest version!	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20033	XM_005207460.4 Latest version!	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20033	XM_003586161.5 Latest version!	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20034	XM_005207462.4 Latest version!	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20034	XM_015463633.1 Latest version!	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa53162	XM_015463632.1 Latest version!	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa53162	XM_010805781.3 Latest version!	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa20033	XM_003586161.4	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20033	XM_005207460.3	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa20034	XM_005207462.3	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa53162	XM_010805781.2	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

EFCAB6 ( XM_003586161.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586209.2 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20033
Clone ID Related Accession (Same CDS sequence)	XM_003586161.4 , XM_015463631.1 , XM_005207460.3 , XM_003586161.5 , XM_005207460.4 , XM_015463630.1
Accession Version	XM_003586161.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586161.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586209.2
CDS	369..4862
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLALVEKFNLDISRDECQQLLIKYDLKNNGKFAYCSFIQSCVLLLKAKETSLMQRMKIQNANKMKEAGAETCSFYSALLRIQPKIVHCWRPMRRTFKAYDEGGTGLLSVADFRKVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFLRAFLQ

Target ORF information:

RefSeq Version	XM_003586161.4
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586161.4

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_015463631.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319117.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20033
Clone ID Related Accession (Same CDS sequence)	XM_003586161.4 , XM_015463631.1 , XM_005207460.3 , XM_003586161.5 , XM_005207460.4 , XM_015463630.1
Accession Version	XM_015463631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644862.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319117.1
CDS	459..4952
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLALVEKFNLDISRDECQQLLIKYDLKNNGKFAYCSFIQSCVLLLKAKETSLMQRMKIQNANKMKEAGAETCSFYSALLRIQPKIVHCWRPMRRTFKAYDEGGTGLLSVADFRKVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFLRAFLQ

Target ORF information:

RefSeq Version	XM_015463631.1
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463631.1

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_005207460.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207517.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.

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CloneID	OBa20033
Clone ID Related Accession (Same CDS sequence)	XM_003586161.4 , XM_015463631.1 , XM_005207460.3 , XM_003586161.5 , XM_005207460.4 , XM_015463630.1
Accession Version	XM_005207460.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207517.1
CDS	459..4952
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLALVEKFNLDISRDECQQLLIKYDLKNNGKFAYCSFIQSCVLLLKAKETSLMQRMKIQNANKMKEAGAETCSFYSALLRIQPKIVHCWRPMRRTFKAYDEGGTGLLSVADFRKVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFLRAFLQ

Target ORF information:

RefSeq Version	XM_005207460.3
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207460.3

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_003586161.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586209.2 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

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CloneID	OBa20033
Clone ID Related Accession (Same CDS sequence)	XM_003586161.4 , XM_015463631.1 , XM_005207460.3 , XM_003586161.5 , XM_005207460.4 , XM_015463630.1
Accession Version	XM_003586161.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586161.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586209.2
CDS	374..4867
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLALVEKFNLDISRDECQQLLIKYDLKNNGKFAYCSFIQSCVLLLKAKETSLMQRMKIQNANKMKEAGAETCSFYSALLRIQPKIVHCWRPMRRTFKAYDEGGTGLLSVADFRKVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFLRAFLQ

Target ORF information:

RefSeq Version	XM_003586161.5
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586161.5

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_005207460.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207517.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.

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CloneID	OBa20033
Clone ID Related Accession (Same CDS sequence)	XM_003586161.4 , XM_015463631.1 , XM_005207460.3 , XM_003586161.5 , XM_005207460.4 , XM_015463630.1
Accession Version	XM_005207460.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207460.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207517.1
CDS	465..4958
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLALVEKFNLDISRDECQQLLIKYDLKNNGKFAYCSFIQSCVLLLKAKETSLMQRMKIQNANKMKEAGAETCSFYSALLRIQPKIVHCWRPMRRTFKAYDEGGTGLLSVADFRKVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFLRAFLQ

Target ORF information:

RefSeq Version	XM_005207460.4
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207460.4

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_015463630.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319116.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

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CloneID	OBa20033
Clone ID Related Accession (Same CDS sequence)	XM_003586161.4 , XM_015463631.1 , XM_005207460.3 , XM_003586161.5 , XM_005207460.4 , XM_015463630.1
Accession Version	XM_015463630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644862.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319116.1
CDS	369..4862
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLALVEKFNLDISRDECQQLLIKYDLKNNGKFAYCSFIQSCVLLLKAKETSLMQRMKIQNANKMKEAGAETCSFYSALLRIQPKIVHCWRPMRRTFKAYDEGGTGLLSVADFRKVLRQYSINLSEEEFFHILEYYDKTLSSKISYNDFLRAFLQ

Target ORF information:

RefSeq Version	XM_015463630.1
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463630.1

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAAGAAGCC GGCGCCGAGA CCTGCTCCTT TTACTCTGCC 
TTGCTTCGGA TTCAGCCCAA GATCGTGCAC TGCTGGAGGC CGATGCGGCG CACGTTCAAG 
GCCTACGATG AGGGCGGGAC GGGGCTTCTC AGCGTGGCTG ACTTCAGGAA GGTCCTGAGG 
CAGTACAGCA TCAACCTCTC CGAGGAGGAG TTCTTCCACA TCCTGGAGTA TTACGACAAG 
ACTCTGTCTT CAAAAATCTC CTACAACGAC TTCCTCAGGG CTTTCCTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_005207462.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207519.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa20034
Clone ID Related Accession (Same CDS sequence)	XM_005207462.4 , XM_015463633.1 , XM_005207462.3
Accession Version	XM_005207462.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207462.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGAAAG AAGCCGGCGC CGAGACCTGC TCCTTTTACT CTGCCTTGCT 
TCGGATTCAG CCCAAGATCG TGCACTGCTG GAGGCCGATG CGGCGCACGT TCAAGGCCTA 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207519.1
CDS	376..4521
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLERSRRRDLLLLLCLASDSAQDRALLEADAAHVQGLR

Target ORF information:

RefSeq Version	XM_005207462.4
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207462.4

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGAAAG AAGCCGGCGC CGAGACCTGC TCCTTTTACT CTGCCTTGCT 
TCGGATTCAG CCCAAGATCG TGCACTGCTG GAGGCCGATG CGGCGCACGT TCAAGGCCTA 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_015463633.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319119.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

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CloneID	OBa20034
Clone ID Related Accession (Same CDS sequence)	XM_005207462.4 , XM_015463633.1 , XM_005207462.3
Accession Version	XM_015463633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644862.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGAAAG AAGCCGGCGC CGAGACCTGC TCCTTTTACT CTGCCTTGCT 
TCGGATTCAG CCCAAGATCG TGCACTGCTG GAGGCCGATG CGGCGCACGT TCAAGGCCTA 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319119.1
CDS	370..4515
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLERSRRRDLLLLLCLASDSAQDRALLEADAAHVQGLR

Target ORF information:

RefSeq Version	XM_015463633.1
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463633.1

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGAAAG AAGCCGGCGC CGAGACCTGC TCCTTTTACT CTGCCTTGCT 
TCGGATTCAG CCCAAGATCG TGCACTGCTG GAGGCCGATG CGGCGCACGT TCAAGGCCTA 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_005207462.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207519.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa20034
Clone ID Related Accession (Same CDS sequence)	XM_005207462.4 , XM_015463633.1 , XM_005207462.3
Accession Version	XM_005207462.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207462.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGAAAG AAGCCGGCGC CGAGACCTGC TCCTTTTACT CTGCCTTGCT 
TCGGATTCAG CCCAAGATCG TGCACTGCTG GAGGCCGATG CGGCGCACGT TCAAGGCCTA 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207519.1
CDS	370..4515
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLERSRRRDLLLLLCLASDSAQDRALLEADAAHVQGLR

Target ORF information:

RefSeq Version	XM_005207462.3
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207462.3

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGAAAG AAGCCGGCGC CGAGACCTGC TCCTTTTACT CTGCCTTGCT 
TCGGATTCAG CCCAAGATCG TGCACTGCTG GAGGCCGATG CGGCGCACGT TCAAGGCCTA 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_010805781.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804083.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa53162
Clone ID Related Accession (Same CDS sequence)	XM_010805781.2 , XM_015463632.1 , XM_010805781.3
Accession Version	XM_010805781.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103944.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAGCCGGCG CCGAGACCTG CTCCTTTTAC TCTGCCTTGC 
TTCGGATTCA GCCCAAGATC GTGCACTGCT GGAGGCCGAT GCGGCGCACG TTCAAGGCCT 
ACGATGAGGG CGGGACGGGG CTTCTCAGCG TGGCTGACTT CAGGAAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804083.1
CDS	369..4742
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLALVEKFNLDISRDECQQLLIKYDLKNNGKFAYCSFIQSCVLLLKAKETSLMQRMKIQNANKMKPAPRPAPFTLPCFGFSPRSCTAGGRCGARSRPTMRAGRGFSAWLTSGRS

Target ORF information:

RefSeq Version	XM_010805781.2
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805781.2

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAGCCGGCG CCGAGACCTG CTCCTTTTAC TCTGCCTTGC 
TTCGGATTCA GCCCAAGATC GTGCACTGCT GGAGGCCGAT GCGGCGCACG TTCAAGGCCT 
ACGATGAGGG CGGGACGGGG CTTCTCAGCG TGGCTGACTT CAGGAAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_015463632.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319118.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.

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CloneID	OBa53162
Clone ID Related Accession (Same CDS sequence)	XM_010805781.2 , XM_015463632.1 , XM_010805781.3
Accession Version	XM_015463632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644862.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAGCCGGCG CCGAGACCTG CTCCTTTTAC TCTGCCTTGC 
TTCGGATTCA GCCCAAGATC GTGCACTGCT GGAGGCCGAT GCGGCGCACG TTCAAGGCCT 
ACGATGAGGG CGGGACGGGG CTTCTCAGCG TGGCTGACTT CAGGAAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319118.1
CDS	369..4742
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLALVEKFNLDISRDECQQLLIKYDLKNNGKFAYCSFIQSCVLLLKAKETSLMQRMKIQNANKMKPAPRPAPFTLPCFGFSPRSCTAGGRCGARSRPTMRAGRGFSAWLTSGRS

Target ORF information:

RefSeq Version	XM_015463632.1
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463632.1

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAGCCGGCG CCGAGACCTG CTCCTTTTAC TCTGCCTTGC 
TTCGGATTCA GCCCAAGATC GTGCACTGCT GGAGGCCGAT GCGGCGCACG TTCAAGGCCT 
ACGATGAGGG CGGGACGGGG CTTCTCAGCG TGGCTGACTT CAGGAAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFCAB6 ( XM_010805781.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804083.1 Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.

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CloneID	OBa53162
Clone ID Related Accession (Same CDS sequence)	XM_010805781.2 , XM_015463632.1 , XM_010805781.3
Accession Version	XM_010805781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	EF-hand calcium-binding domain-containing protein 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAGCCGGCG CCGAGACCTG CTCCTTTTAC TCTGCCTTGC 
TTCGGATTCA GCCCAAGATC GTGCACTGCT GGAGGCCGAT GCGGCGCACG TTCAAGGCCT 
ACGATGAGGG CGGGACGGGG CTTCTCAGCG TGGCTGACTT CAGGAAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804083.1
CDS	375..4748
Translation	MCKMAVVPDWHIICPYTRKFTHSRPHSSPCRVYSRSVFQNMFRPSSSTTVANPILSSLDVKRILFQKITEKGDELKRAFHLLDTANNMTVTKSELRRVITTFLLPLTREQFQDVLAQIPLTSSGAVPYLVFLSRFGGIDLNINVIKRGGENEMNGCRTLKDLEAQVGEKIFKNIKTVIKALMLIDVNTTGLVQPHELRRVLETFCLKMKDEEYKKFAQHYNIDKDAAVDYNVFLKNLGSNNDLNLKYNMGSQEVLWENQRVKSFKREEPPSSAVSDNIWKNYSLDEIETAFCLELSKCYEKVEKALSAGDPCTSGYVSLNYLKIVLDTFVYRLPRRIFIQLMKRFGLKTTTKVNWKQFLTSFYEPQYEASSKAPLTKRNSINFRNQVHKENVITKLFRHEDHDSSLKKALLLIKSKPDGQITGQELQRILNCMVVKISDSEFRELMRILDPGCTGCVNVSRFIELIEESPKLHKIPAYKDTKMPLFLAWDSVEEIIHDSIAKNLSAFCNMLRSYDLGDTGLIGRNNFKKIMRVFCPFLTTEHLVKLCSKFQDIASGRILYKKLLACLGINRPPTVSPVSMPKGQLLNEHLQKEEQPLPDHSERATPAESQGAVTRNMTKDEVIEKLKSCIQQQDPAFRKRFLDFTKEPNGKIHTHDFKKVLEDYGMPMDDDQYALLTTKLGYKKEGMSYLDFAAGFEDPKMNEPEVTPPQTPVLPKGDVDSFFVTAEECLRQFPRRLKEFFRDPYAAFFKMDTDRDGILTMHDLHRLLQHLLFNLKDEEFERLLGLLGLRLSITLNFREFRNLCEKRSFSADDDAPQRLPRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDSEGNGILRRRDMKNALYGFDIPLTPREFEKLWMRYDSEGRGHITYQEFLQKLGINYSADIHRPYAEEYFNFMGHFTKPQQVQEELKELQQSTEKAMPARDKLKDHDQDISKALAKLDKSRTGYISLGRLQRLLQECGCSLKEEELIDLLNSWGIAWHNNSINYLDFLRAVENNKLTRPQSQEKEESVPINFAALSPEEVLKNIQGVIAASSLALSTAFSALDKEDTGFVKASDFGQVLKDFCYKLTDNQYHYFLRKLRLHLTPYINWKYFLQNFSSYVEETAVEWAEKMPRGPRPLSPKEMANQELLARLHKAVTSHYHAIAQEFENFDTMKTNTASRDEFRSICTRHVQVLTDEQFDRLWEEMPVNSKGRLRYLDFLSSFNVDKQATPLATSDSAKAQRGSSVPEVSDRGRSAGSSPTRDPKAGSKPRSHPCTPSSVSGTPPLQNCEPIETKLRKKVQGCWRELLRECKERDFNKQGEIPGPEFLALVEKFNLDISRDECQQLLIKYDLKNNGKFAYCSFIQSCVLLLKAKETSLMQRMKIQNANKMKPAPRPAPFTLPCFGFSPRSCTAGGRCGARSRPTMRAGRGFSAWLTSGRS

Target ORF information:

RefSeq Version	XM_010805781.3
Organism	Bos taurus(cattle)
Definition	Bos taurus EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805781.3

ORF Insert Sequence:

ATGTGCAAAA TGGCAGTTGT ACCAGATTGG CATATTATAT GTCCTTACAC ACGGAAATTC 
ACACATTCAA GACCGCACTC TTCACCATGC AGAGTGTATT CAAGGAGTGT TTTCCAAAAT 
ATGTTCAGAC CTTCTTCATC CACAACTGTT GCAAATCCAA TACTGTCGTC CTTAGATGTT 
AAACGGATTT TATTTCAAAA AATTACCGAG AAAGGAGATG AGTTGAAGAG AGCCTTTCAC 
CTGCTCGACA CCGCTAACAA CATGACGGTG ACCAAGAGTG AACTGCGGAG GGTCATCACA 
ACCTTCCTGC TGCCTCTCAC GAGAGAACAG TTTCAGGATG TTCTGGCTCA GATCCCCCTC 
ACCAGTTCTG GGGCTGTTCC GTACCTTGTA TTCCTGTCCA GATTTGGAGG AATTGACCTG 
AATATCAATG TTATAAAGAG GGGAGGTGAG AATGAAATGA ATGGCTGTCG GACGTTAAAA 
GACCTCGAGG CCCAAGTAGG AGAAAAGATT TTCAAAAACA TTAAAACTGT CATCAAAGCC 
CTTATGCTCA TTGATGTTAA CACAACTGGT CTGGTCCAGC CACACGAGCT GAGGAGGGTT 
CTGGAGACCT TCTGTTTGAA GATGAAAGAT GAGGAATACA AAAAGTTCGC ACAACACTAC 
AATATCGATA AAGACGCTGC AGTGGATTAT AACGTTTTCT TGAAGAACCT CGGTTCAAAT 
AACGACTTGA ACCTTAAATA TAATATGGGA AGTCAAGAGG TCCTGTGGGA GAATCAACGA 
GTCAAAAGTT TCAAAAGAGA GGAACCCCCC AGTTCCGCTG TATCTGACAA TATCTGGAAA 
AACTACTCCC TGGATGAAAT TGAGACGGCC TTTTGCCTAG AGCTTTCCAA GTGCTATGAA 
AAGGTTGAGA AGGCGCTGAG TGCAGGGGAC CCCTGCACAA GTGGATATGT GTCTTTGAAT 
TACCTAAAGA TTGTCCTCGA CACCTTTGTA TACCGATTAC CAAGAAGAAT TTTCATCCAG 
TTAATGAAAA GATTTGGACT TAAAACCACC ACTAAAGTCA ATTGGAAGCA ATTTTTAACA 
TCATTTTATG AACCTCAATA TGAAGCTAGC AGTAAAGCTC CCTTGACAAA AAGAAATAGC 
ATCAACTTTA GAAATCAGGT TCACAAGGAA AACGTTATCA CGAAGTTATT TAGACATGAA 
GATCACGATT CATCGTTGAA GAAAGCACTG CTGCTCATCA AGTCTAAACC TGATGGACAG 
ATAACAGGGC AAGAACTGCA ACGCATTCTA AATTGCATGG TGGTAAAAAT AAGTGATTCA 
GAATTCAGGG AACTAATGCG AATACTTGAC CCTGGGTGCA CCGGATGTGT CAACGTCAGC 
AGGTTTATTG AGCTAATTGA AGAGAGCCCT AAGCTGCACA AAATACCTGC CTATAAAGAC 
ACCAAGATGC CTCTCTTCCT GGCTTGGGAT TCAGTGGAAG AAATTATTCA TGACTCGATT 
GCTAAGAATC TATCAGCTTT CTGTAATATG CTGCGGTCAT ATGACCTCGG AGACACAGGA 
CTGATTGGGC GAAATAATTT CAAGAAAATC ATGCGTGTAT TCTGCCCATT TTTAACAACT 
GAACATTTAG TAAAACTCTG CAGTAAGTTT CAGGATATTG CTTCAGGAAG AATCCTTTAC 
AAGAAACTTT TGGCATGCCT AGGAATTAAT CGCCCGCCCA CTGTCTCTCC AGTTTCCATG 
CCAAAGGGTC AACTGTTAAA CGAACATCTT CAAAAAGAGG AGCAGCCACT GCCAGACCAT 
TCTGAGAGAG CCACGCCCGC TGAGAGCCAA GGTGCTGTGA CCAGGAACAT GACCAAGGAT 
GAAGTCATTG AAAAGCTCAA AAGCTGTATA CAGCAGCAGG ACCCAGCATT CAGAAAACGA 
TTTCTCGACT TCACCAAGGA GCCTAACGGC AAAATTCACA CACATGACTT CAAGAAGGTC 
CTAGAAGATT ATGGAATGCC CATGGATGAC GATCAGTACG CTCTGTTGAC CACAAAATTA 
GGATATAAGA AAGAAGGGAT GAGTTATCTT GATTTTGCAG CGGGATTCGA AGATCCTAAG 
ATGAACGAGC CAGAAGTGAC CCCACCCCAG ACTCCAGTTC TTCCCAAAGG TGATGTGGAC 
AGCTTCTTTG TCACTGCTGA AGAGTGTCTG AGACAGTTCC CCCGGAGACT GAAGGAGTTC 
TTCCGGGACC CGTACGCCGC CTTCTTTAAG ATGGATACAG ACAGGGACGG CATCCTCACC 
ATGCACGACC TCCACCGCCT GCTGCAGCAC CTGCTCTTCA ACCTGAAGGA TGAGGAGTTC 
GAGCGCCTCC TGGGCCTTCT CGGCCTGAGG CTCAGCATCA CGTTAAACTT CCGGGAGTTT 
CGGAACCTGT GCGAGAAGAG GTCGTTCAGT GCGGATGACG ACGCGCCCCA GAGACTGCCC 
AGACCAAAAC AGAAGGTTGC AGATTCAGAA CTAGCTTGTG AACAGGCGCA TCAGTACCTT 
GTCACCAAAG CAAAAACCAG ATGGTCAGAC TTGTCGAAGA ATTTTATAGA AACTGACAGC 
GAGGGCAACG GTATCCTTCG ACGACGGGAC ATGAAGAACG CGCTGTACGG CTTTGACATC 
CCCCTCACGC CGAGAGAGTT TGAAAAGCTC TGGATGAGGT ACGACAGTGA GGGAAGAGGG 
CACATCACTT ACCAAGAATT TTTGCAGAAA CTGGGGATTA ACTACTCAGC AGATATTCAC 
CGGCCCTACG CGGAGGAGTA CTTTAACTTC ATGGGTCATT TCACGAAGCC TCAGCAGGTG 
CAAGAAGAGC TGAAGGAGCT GCAGCAGAGC ACAGAGAAGG CCATGCCAGC CAGGGATAAA 
CTTAAGGACC ACGATCAAGA TATCAGCAAA GCACTTGCCA AACTAGATAA ATCCAGAACT 
GGCTACATAT CCTTGGGCAG GCTGCAGAGG CTGCTGCAGG AGTGTGGCTG TTCTCTGAAG 
GAAGAGGAGC TGATCGATCT TCTAAACAGT TGGGGAATCG CCTGGCACAA TAACTCCATC 
AATTACCTTG ACTTCTTGCG AGCGGTGGAG AACAACAAGC TGACAAGACC TCAGTCCCAG 
GAGAAAGAGG AGAGCGTGCC GATCAATTTT GCAGCCCTAA GTCCAGAGGA GGTTCTGAAG 
AACATCCAGG GAGTCATCGC CGCCTCCAGT CTGGCTTTGT CAACGGCATT TTCTGCATTG 
GATAAAGAGG ACACTGGGTT TGTAAAGGCT TCAGATTTTG GACAGGTTCT TAAGGATTTC 
TGTTACAAAC TAACAGATAA CCAGTATCAT TATTTTTTGA GAAAACTCAG ACTTCATCTC 
ACCCCCTACA TAAATTGGAA ATATTTTCTC CAGAACTTCA GCAGTTACGT TGAGGAGACC 
GCTGTGGAGT GGGCTGAGAA GATGCCCAGA GGTCCACGGC CCTTATCGCC CAAGGAAATG 
GCAAACCAGG AGCTGCTGGC TCGCCTGCAC AAGGCCGTGA CCTCCCACTA CCACGCCATC 
GCCCAGGAGT TCGAGAACTT TGACACCATG AAGACGAACA CCGCCTCCAG GGACGAGTTC 
CGGTCCATCT GTACTCGCCA TGTCCAGGTT CTAACGGACG AGCAATTTGA CAGGCTCTGG 
GAGGAGATGC CCGTCAACTC CAAGGGGAGG CTGAGGTACC TGGACTTCCT GAGCAGCTTC 
AACGTGGACA AGCAAGCCAC GCCGCTGGCC ACGAGTGACT CAGCCAAGGC GCAGAGAGGG 
AGCAGTGTCC CCGAGGTCTC GGACCGGGGC CGGTCCGCAG GGTCGTCGCC CACTCGCGAC 
CCAAAAGCGG GGTCCAAGCC ACGGAGTCAC CCCTGCACCC CCTCGAGCGT GAGCGGGACC 
CCACCCCTGC AGAACTGCGA GCCCATCGAG ACCAAGCTTC GCAAGAAGGT CCAGGGCTGC 
TGGCGCGAGC TGCTACGCGA GTGCAAGGAG AGGGACTTCA ACAAGCAGGG CGAGATCCCC 
GGCCCCGAGT TCCTGGCTCT CGTGGAGAAA TTCAACCTGG ACATCAGCAG AGACGAGTGC 
CAGCAGCTCC TCATCAAGTA CGACTTGAAG AACAACGGGA AGTTCGCTTA CTGCAGCTTC 
ATCCAGAGCT GCGTCCTCCT GTTAAAAGCA AAGGAGACCT CACTGATGCA GAGAATGAAA 
ATCCAGAACG CGAATAAAAT GAAGCCGGCG CCGAGACCTG CTCCTTTTAC TCTGCCTTGC 
TTCGGATTCA GCCCAAGATC GTGCACTGCT GGAGGCCGAT GCGGCGCACG TTCAAGGCCT 
ACGATGAGGG CGGGACGGGG CTTCTCAGCG TGGCTGACTT CAGGAAGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EFCAB6
Entrez Gene ID	535401
Full Name	EF-hand calcium binding domain 6
General protein information	Preferred Names EF-hand calcium binding domain 6 Names EF-hand calcium-binding domain-containing protein 6 CAP-binding protein complex interacting protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	EFCAB6	XP_515181.2
Bos taurus (cattle)	EFCAB6	XP_003586209.2
Danio rerio (zebrafish)	im:7142172	XP_003201483.2
Canis lupus familiaris (dog)	EFCAB6	XP_005625826.1
Mus musculus (house mouse)	Efcab6	NP_084222.4
Rattus norvegicus (Norway rat)	Efcab6	XP_006242207.1
Homo sapiens (human)	EFCAB6	NP_073622.2
	EFCAB6	XP_001108976.1
Xenopus tropicalis (tropical clawed frog)	efcab6	XP_002942488.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EFCAB6 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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