EFCAB6 cDNA ORF clone, Canis lupus familiaris(dog)

The following EFCAB6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFCAB6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe291393 XM_022424330.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe291394 XM_022424331.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe291395 XM_022424332.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe38866 XM_014117294.2
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe38867 XM_014117295.2
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe38868 XM_022424333.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe38868 XM_014117296.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe13362 XM_005625769.2
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe13363 XM_022424329.1
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe13363 XM_538339.6
Latest version!
Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OCe38867 XM_014117295.1 Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe38866 XM_014117294.1 Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe13363 XM_538339.5 Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe291393
    Clone ID Related Accession (Same CDS sequence) XM_022424330.1
    Accession Version XM_022424330.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATCCCC
    CTCACCAGCT CTGGGGCCAT TCCATACCTT GAATTCCTGT CCTGGTTTGG TGGAATGGAC
    CTAAGTATAA ATGTTATACA GAGGGGAGGT GGGAATGAAA TGAGTTGCAG TCGGACAGTA
    AAAGAACTTG AAAAACAAAT AGGAGAAAAG ATTTTCAGAA ACATTAAAAC TATTTTCAAA
    GCCTTCAAGC TCATTGATGT TAGCAAGACT GGTCTGATCC AGTCTTGTGA ACTGAGGAAA
    GTTCTCGAGA CCTTCTGTTT GAAGATGAAA GATGATGAAT ACAAAAAGTT TGCAAAACAC
    TACAACATTG ATAAAGATAC TGCAGTGGAT TACAATGTTT TTTTGAAGAA CCTCAGTGCA
    AATAATGACT TGAACCTTAG ATATTATATG GGAAACCAAG ATATCTCCTG GAAGAATCAA
    CCAGTCCAAA ATTCCAAAGG GGAGTGCCTC CACAGTTCTG GTTCATCTGA AGATGTCTGG
    AAAAACTACA ACCTAGACGA AATTGAGAGA ACTTTTTGCC AAGAGCTTCC CAAGTGCTAT
    GAAAAGGTTG AAAAAGCCCT GAGTGCAGGG GACCCCTCCA AAAGTGGATA TGTATCTTTG
    AATTACCTAA AGATTGTCCT CGACACCTTT GTATACCGAT TACCAAGGAG AATTTTTATC
    CAGTTAATGA AAAGAATCAC TTCTAGAGAT CAATCTTGCA AGGAAAACAT TATCACAAAG
    TTATCTAGAC AAGAGGATCA CTATACATCA TTGAAGAAAG CACTCCTGAT CGCCAACACT
    AAACCTGATG GACAGATAAC GGGGAAAGAA TTGCGATATA TTCTAAATTG CATGGTTGTA
    AAAATCAGTG ATTCAGAATT CAAAGAGCTC ATGCAAATGC TTGACCCTGG GGACACCGGA
    TGGGTTAACA TCTACACACT TATTGAACTG CTTGAAGAGA CCCCTAAGAT ACAAAAACTC
    TCCTCCTCTG CAGACACCAA GGCGCCTCTC CTCCTGGCTT GGGATTCAGT GGAAGAAATC
    ATTCAGGACT CAATTGCTAG GAATCTCCAA GCCTTCTACA AAATGCTACG GTCATATGAC
    CTCGGAGACA CAGGGCTCAT TGGTCGAAAT AATTTCAAGA AAATCATGCG CATCTTCTGT
    CCATTTTTAA CAATCGAACA TTTACTGAAA CTCTGTAGTA GGTTTCAGGA TATTGCTTCA
    GGAAGAATCC TTTATAAGAA ATTTTTGGCA TCCATGGGGA TGAGTGGCCC ACCACCCCCC
    TCTCCGGTTA TTACTCCAAA GGATCAGCTA TTGAGTGACC ATTTTCAAAG AGAGGAGCAG
    CGGAAGTTGG ATCTTTCTGA AAGAACCAAG CCCGTGGAGG ACAGAAGCAC CTCGACCAAG
    AAAATGACGA AGGATGAAGC GATCGAAAAA CTAAAAAACT GTATTCAGCA GCGGGACCCA
    GCATTCAAAA AATTCTTTCT TGACTATAGC AAGGAGCTCA CTGGCAAAAT TCATGTGCGT
    GACTTCAAGA AGGTACTGGA AGCTAGTGGA ATGCCCATGG ATGATGACCA GTACGCTCTG
    CTGACGGCAA AAATAGGATA TAAGAGAGAA GGGATGAGTT ATCTTGATTT TGCAGCAGGA
    TTTGAAGATG CTAAAATGAG CAGGCCAGAA GTGATCCCAT TGCAGACTCC AGTTCTTTCC
    AAGACTAATT TGGACAGATA CTTTATAACC GCTGAAGAGT GCCTGAAGCA GTTCCCCCAG
    AGACTGAAGG AAGCCTTCCG GGACCCCTAC TCTGCCTTCT TTAAAATAGA CACTGACAGG
    GATGGCATAA TCAACATGCA TGATCTTCAC AGATTGTTAC TGCACTTACT GTTCAATCTT
    AAAGACAAAG AATTTGAGCG TTTCCTGGGC CTTCTTGGCT TGAGACTCAG CGTCACTCTC
    AATTTTCGGG AATTTCGAAA TCTGTGTGAG AAGAGAGCAT TGAGAACAGA TGACGCGCCT
    CAAAGACTAA TCAGACCAAA ACAGAAGGTC GCAGATTCAG AACTAGCTTG TGAACAGGCT
    CATCAGTATC TTGTTACCAA AGCAAAAACC AGATGGTCAG ACTTATCTAA GAATTTTATA
    GAAACTGATA ATGAGGGCAA TGGCATCCTT CGGCGGCGGG ATATAAAGAA TGCGCTCTAT
    GGATTTGATA TTCCTCTGAC ACCGAGGGAA TTTGAAAAGC TCTGGATGAG CTATGACACC
    GAGGGAAGAG GGCACATAAC TTACCAAGAA TTTCTACAGA AATTGGGGAT TAACTACTCA
    GCAGACATTC ATCGGCCCTA TGCAGAGGAT TACTTCAACT TCATGGGTCA TTTCACAAAA
    CCCCAGCAGA TGCAAGAGGA GGCCAGACAG CTAGAGCAGA GCGTGGACAG GACTGCGCCA
    GTTAGGGATA AGCTTAAGGA CCATTATCAA GATATCAGCA AAGCACTCAC CAAACTAGAC
    AAATCCAAAA ATGGCTACCT ATCCATGTGC AAGATGCAGA AAGTGCTGCA GGAGCTCGGC
    TGCTCTCTCA AGGAGGAGGA GCTGACCAAT CTTCTGAACA GTTGGGAAAT CAAATGGCAT
    GATAACTCCA TCAATTACCT TGACTTCCTG AAAGCAGTGG AGAGCAGCAA GCCAACAAGA
    CCTCAGCCAA AGAAAAAAGA AGAGAGTGTG CCAATCAGTT TCTCAACACT GAACCCAGAG
    GAGGTTGTAA AGAACATCCA GGAAGTTGTT GCCTCCTCCG ACATGGCTTT GTCAACGGCA
    TTTTCTGCAT TGGATAAAGA GGACACTGGG TTTGTAAAGG CTTCAGATTT TGGACAAGTT
    CTTAAGGATT TCTGTTACAA ACTAACAGAC AACCAGTATC ATTATTTTTT GAGAAAACTG
    AAAATTCATC TAACCCCCCA TATAAATTGG AAATATTTTC TCCAGAACTT CAGTTCTTTC
    TCTGAAGAGA CTGCTGCCGA GTGGGCTGAG AAAATGCCCA AAGGCCCACC GCCCACATCT
    CCTCCCAAGG AAACAGCAAA CCAGGAGCTG CTGGCTCAGC TCCACAAGGC GGTGGGCTCC
    CACTACCATG CGATTGCTCA GGAGTTTGAC AACTTCGACA CCATGAAAAC GTACACCGCC
    TCCAAGGATG AATTCAGGGC CATTTGTGCT CGCCATGTCC AAATTCTGAC AGATGAGCAA
    TTTGACAGAC TGTGGAGCAA GATGCCAGTC AATGCCAAGG GGAGGCTGAA GTACCTGGAC
    CTCCTGAGCA TGTTCAGCTC CAAGAGAGCG CCAACACCAT TGACCACGGG CAGCTCGGCC
    AAGGCCCAGA GAGGCAGCAG TGTTCCTGAA ATCTCAGATG GAAGCAGGCC TGCAGTATCA
    TCGCCCATTC ATGACCTGAA GGCGGGGTTG AAAGCACAGA GCCACCCCTG TACCCCCGCT
    AGTGCCAGTT CGGCGCCAGG CACCCTGCCC TTGCAGAACT GCGAGCCCAT AGAAAGCAAG
    CTCCAGAAGA AAATCCAGGG CTGCTGGCGA GAGTTCCTGA AAGAATGCAA AGAGAAGGAT
    GTGAATAAGC AGGGCCAGGT CACCACGGCT GAATTCCTGG CTCTTGTGGA GAAATTCAAT
    CTGGACATAA GCAAAGAGGA ATGTCAGCAG CTCATCGTGA AGTATGATTT AAAGAACAAC
    GGGAAGTTTG CTTATTGCAA CTTCATCCAG AGCTGTGTCC TCCTGTTGAA AGCGAAGGAA
    ACCTCACTGA TGCAGAGGAT GAAAATTCAG AATGCACATA AAATGAAAGA ATCTGGTGCT
    GAGACTTCTT CCTTTTATTC AGCTTTACTG CGTATCCAGC CCAAAATTGT GCACTGCTGG
    AGGCCCATGC GGCGTACCTT CAAAGCCTAT GATGAGGGCG GGACAGGGCT TTTAAGCGTG
    GCTGATTTCA GGAAGGTCCT GAGACAGTAC AGCATCAACC TCTCGGAGGA GGAGTTCTTT
    CACATTCTGG AGTATTATGA CAAGACGCTG TCTTCCAAGA TCTCCTACAA CGACTTCCTG
    CGGGCGTTCC TTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280038.1
    CDS33..4430
    Translation

    Target ORF information:

    RefSeq Version XM_022424330.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATCCCC
    CTCACCAGCT CTGGGGCCAT TCCATACCTT GAATTCCTGT CCTGGTTTGG TGGAATGGAC
    CTAAGTATAA ATGTTATACA GAGGGGAGGT GGGAATGAAA TGAGTTGCAG TCGGACAGTA
    AAAGAACTTG AAAAACAAAT AGGAGAAAAG ATTTTCAGAA ACATTAAAAC TATTTTCAAA
    GCCTTCAAGC TCATTGATGT TAGCAAGACT GGTCTGATCC AGTCTTGTGA ACTGAGGAAA
    GTTCTCGAGA CCTTCTGTTT GAAGATGAAA GATGATGAAT ACAAAAAGTT TGCAAAACAC
    TACAACATTG ATAAAGATAC TGCAGTGGAT TACAATGTTT TTTTGAAGAA CCTCAGTGCA
    AATAATGACT TGAACCTTAG ATATTATATG GGAAACCAAG ATATCTCCTG GAAGAATCAA
    CCAGTCCAAA ATTCCAAAGG GGAGTGCCTC CACAGTTCTG GTTCATCTGA AGATGTCTGG
    AAAAACTACA ACCTAGACGA AATTGAGAGA ACTTTTTGCC AAGAGCTTCC CAAGTGCTAT
    GAAAAGGTTG AAAAAGCCCT GAGTGCAGGG GACCCCTCCA AAAGTGGATA TGTATCTTTG
    AATTACCTAA AGATTGTCCT CGACACCTTT GTATACCGAT TACCAAGGAG AATTTTTATC
    CAGTTAATGA AAAGAATCAC TTCTAGAGAT CAATCTTGCA AGGAAAACAT TATCACAAAG
    TTATCTAGAC AAGAGGATCA CTATACATCA TTGAAGAAAG CACTCCTGAT CGCCAACACT
    AAACCTGATG GACAGATAAC GGGGAAAGAA TTGCGATATA TTCTAAATTG CATGGTTGTA
    AAAATCAGTG ATTCAGAATT CAAAGAGCTC ATGCAAATGC TTGACCCTGG GGACACCGGA
    TGGGTTAACA TCTACACACT TATTGAACTG CTTGAAGAGA CCCCTAAGAT ACAAAAACTC
    TCCTCCTCTG CAGACACCAA GGCGCCTCTC CTCCTGGCTT GGGATTCAGT GGAAGAAATC
    ATTCAGGACT CAATTGCTAG GAATCTCCAA GCCTTCTACA AAATGCTACG GTCATATGAC
    CTCGGAGACA CAGGGCTCAT TGGTCGAAAT AATTTCAAGA AAATCATGCG CATCTTCTGT
    CCATTTTTAA CAATCGAACA TTTACTGAAA CTCTGTAGTA GGTTTCAGGA TATTGCTTCA
    GGAAGAATCC TTTATAAGAA ATTTTTGGCA TCCATGGGGA TGAGTGGCCC ACCACCCCCC
    TCTCCGGTTA TTACTCCAAA GGATCAGCTA TTGAGTGACC ATTTTCAAAG AGAGGAGCAG
    CGGAAGTTGG ATCTTTCTGA AAGAACCAAG CCCGTGGAGG ACAGAAGCAC CTCGACCAAG
    AAAATGACGA AGGATGAAGC GATCGAAAAA CTAAAAAACT GTATTCAGCA GCGGGACCCA
    GCATTCAAAA AATTCTTTCT TGACTATAGC AAGGAGCTCA CTGGCAAAAT TCATGTGCGT
    GACTTCAAGA AGGTACTGGA AGCTAGTGGA ATGCCCATGG ATGATGACCA GTACGCTCTG
    CTGACGGCAA AAATAGGATA TAAGAGAGAA GGGATGAGTT ATCTTGATTT TGCAGCAGGA
    TTTGAAGATG CTAAAATGAG CAGGCCAGAA GTGATCCCAT TGCAGACTCC AGTTCTTTCC
    AAGACTAATT TGGACAGATA CTTTATAACC GCTGAAGAGT GCCTGAAGCA GTTCCCCCAG
    AGACTGAAGG AAGCCTTCCG GGACCCCTAC TCTGCCTTCT TTAAAATAGA CACTGACAGG
    GATGGCATAA TCAACATGCA TGATCTTCAC AGATTGTTAC TGCACTTACT GTTCAATCTT
    AAAGACAAAG AATTTGAGCG TTTCCTGGGC CTTCTTGGCT TGAGACTCAG CGTCACTCTC
    AATTTTCGGG AATTTCGAAA TCTGTGTGAG AAGAGAGCAT TGAGAACAGA TGACGCGCCT
    CAAAGACTAA TCAGACCAAA ACAGAAGGTC GCAGATTCAG AACTAGCTTG TGAACAGGCT
    CATCAGTATC TTGTTACCAA AGCAAAAACC AGATGGTCAG ACTTATCTAA GAATTTTATA
    GAAACTGATA ATGAGGGCAA TGGCATCCTT CGGCGGCGGG ATATAAAGAA TGCGCTCTAT
    GGATTTGATA TTCCTCTGAC ACCGAGGGAA TTTGAAAAGC TCTGGATGAG CTATGACACC
    GAGGGAAGAG GGCACATAAC TTACCAAGAA TTTCTACAGA AATTGGGGAT TAACTACTCA
    GCAGACATTC ATCGGCCCTA TGCAGAGGAT TACTTCAACT TCATGGGTCA TTTCACAAAA
    CCCCAGCAGA TGCAAGAGGA GGCCAGACAG CTAGAGCAGA GCGTGGACAG GACTGCGCCA
    GTTAGGGATA AGCTTAAGGA CCATTATCAA GATATCAGCA AAGCACTCAC CAAACTAGAC
    AAATCCAAAA ATGGCTACCT ATCCATGTGC AAGATGCAGA AAGTGCTGCA GGAGCTCGGC
    TGCTCTCTCA AGGAGGAGGA GCTGACCAAT CTTCTGAACA GTTGGGAAAT CAAATGGCAT
    GATAACTCCA TCAATTACCT TGACTTCCTG AAAGCAGTGG AGAGCAGCAA GCCAACAAGA
    CCTCAGCCAA AGAAAAAAGA AGAGAGTGTG CCAATCAGTT TCTCAACACT GAACCCAGAG
    GAGGTTGTAA AGAACATCCA GGAAGTTGTT GCCTCCTCCG ACATGGCTTT GTCAACGGCA
    TTTTCTGCAT TGGATAAAGA GGACACTGGG TTTGTAAAGG CTTCAGATTT TGGACAAGTT
    CTTAAGGATT TCTGTTACAA ACTAACAGAC AACCAGTATC ATTATTTTTT GAGAAAACTG
    AAAATTCATC TAACCCCCCA TATAAATTGG AAATATTTTC TCCAGAACTT CAGTTCTTTC
    TCTGAAGAGA CTGCTGCCGA GTGGGCTGAG AAAATGCCCA AAGGCCCACC GCCCACATCT
    CCTCCCAAGG AAACAGCAAA CCAGGAGCTG CTGGCTCAGC TCCACAAGGC GGTGGGCTCC
    CACTACCATG CGATTGCTCA GGAGTTTGAC AACTTCGACA CCATGAAAAC GTACACCGCC
    TCCAAGGATG AATTCAGGGC CATTTGTGCT CGCCATGTCC AAATTCTGAC AGATGAGCAA
    TTTGACAGAC TGTGGAGCAA GATGCCAGTC AATGCCAAGG GGAGGCTGAA GTACCTGGAC
    CTCCTGAGCA TGTTCAGCTC CAAGAGAGCG CCAACACCAT TGACCACGGG CAGCTCGGCC
    AAGGCCCAGA GAGGCAGCAG TGTTCCTGAA ATCTCAGATG GAAGCAGGCC TGCAGTATCA
    TCGCCCATTC ATGACCTGAA GGCGGGGTTG AAAGCACAGA GCCACCCCTG TACCCCCGCT
    AGTGCCAGTT CGGCGCCAGG CACCCTGCCC TTGCAGAACT GCGAGCCCAT AGAAAGCAAG
    CTCCAGAAGA AAATCCAGGG CTGCTGGCGA GAGTTCCTGA AAGAATGCAA AGAGAAGGAT
    GTGAATAAGC AGGGCCAGGT CACCACGGCT GAATTCCTGG CTCTTGTGGA GAAATTCAAT
    CTGGACATAA GCAAAGAGGA ATGTCAGCAG CTCATCGTGA AGTATGATTT AAAGAACAAC
    GGGAAGTTTG CTTATTGCAA CTTCATCCAG AGCTGTGTCC TCCTGTTGAA AGCGAAGGAA
    ACCTCACTGA TGCAGAGGAT GAAAATTCAG AATGCACATA AAATGAAAGA ATCTGGTGCT
    GAGACTTCTT CCTTTTATTC AGCTTTACTG CGTATCCAGC CCAAAATTGT GCACTGCTGG
    AGGCCCATGC GGCGTACCTT CAAAGCCTAT GATGAGGGCG GGACAGGGCT TTTAAGCGTG
    GCTGATTTCA GGAAGGTCCT GAGACAGTAC AGCATCAACC TCTCGGAGGA GGAGTTCTTT
    CACATTCTGG AGTATTATGA CAAGACGCTG TCTTCCAAGA TCTCCTACAA CGACTTCCTG
    CGGGCGTTCC TTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291394
    Clone ID Related Accession (Same CDS sequence) XM_022424331.1
    Accession Version XM_022424331.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATTTTC
    AGAAACATTA AAACTATTTT CAAAGCCTTC AAGCTCATTG ATGTTAGCAA GACTGGTCTG
    ATCCAGTCTT GTGAACTGAG GAAAGTTCTC GAGACCTTCT GTTTGAAGAT GAAAGATGAT
    GAATACAAAA AGTTTGCAAA ACACTACAAC ATTGATAAAG ATACTGCAGT GGATTACAAT
    GTTTTTTTGA AGAACCTCAG TGCAAATAAT GACTTGAACC TTAGATATTA TATGGGAAAC
    CAAGATATCT CCTGGAAGAA TCAACCAGTC CAAAATTCCA AAGGGGAGTG CCTCCACAGT
    TCTGGTTCAT CTGAAGATGT CTGGAAAAAC TACAACCTAG ACGAAATTGA GAGAACTTTT
    TGCCAAGAGC TTCCCAAGTG CTATGAAAAG GTTGAAAAAG CCCTGAGTGC AGGGGACCCC
    TCCAAAAGTG GATATGTATC TTTGAATTAC CTAAAGATTG TCCTCGACAC CTTTGTATAC
    CGATTACCAA GGAGAATTTT TATCCAGTTA ATGAAAAGAT TTGGACTTAA AACCACCACC
    AAAATCAATT GGAAACAATT TCTAACATCA CTTTATGAAC CCCAGTGGTT GGAAGTCAAC
    AATACAGTTC CTCCAACAAA AAGAAATAGA ATCACTTCTA GAGATCAATC TTGCAAGGAA
    AACATTATCA CAAAGTTATC TAGACAAGAG GATCACTATA CATCATTGAA GAAAGCACTC
    CTGATCGCCA ACACTAAACC TGATGGACAG ATAACGGGGA AAGAATTGCG ATATATTCTA
    AATTGCATGG TTGTAAAAAT CAGTGATTCA GAATTCAAAG AGCTCATGCA AATGCTTGAC
    CCTGGGGACA CCGGATGGGT TAACATCTAC ACACTTATTG AACTGCTTGA AGAGACCCCT
    AAGATACAAA AACTCTCCTC CTCTGCAGAC ACCAAGGCGC CTCTCCTCCT GGCTTGGGAT
    TCAGTGGAAG AAATCATTCA GGACTCAATT GCTAGGAATC TCCAAGCCTT CTACAAAATG
    CTACGGTCAT ATGACCTCGG AGACACAGGG CTCATTGGTC GAAATAATTT CAAGAAAATC
    ATGCGCATCT TCTGTCCATT TTTAACAATC GAACATTTAC TGAAACTCTG TAGTAGGTTT
    CAGGATATTG CTTCAGGAAG AATCCTTTAT AAGAAATTTT TGGCATCCAT GGGGATGAGT
    GGCCCACCAC CCCCCTCTCC GGTTATTACT CCAAAGGATC AGCTATTGAG TGACCATTTT
    CAAAGAGAGG AGCAGCGGAA GTTGGATCTT TCTGAAAGAA CCAAGCCCGT GGAGGACAGA
    AGCACCTCGA CCAAGAAAAT GACGAAGGAT GAAGCGATCG AAAAACTAAA AAACTGTATT
    CAGCAGCGGG ACCCAGCATT CAAAAAATTC TTTCTTGACT ATAGCAAGGA GCTCACTGGC
    AAAATTCATG TGCGTGACTT CAAGAAGGTA CTGGAAGCTA GTGGAATGCC CATGGATGAT
    GACCAGTACG CTCTGCTGAC GGCAAAAATA GGATATAAGA GAGAAGGGAT GAGTTATCTT
    GATTTTGCAG CAGGATTTGA AGATGCTAAA ATGAGCAGGC CAGAAGTGAT CCCATTGCAG
    ACTCCAGTTC TTTCCAAGAC TAATTTGGAC AGATACTTTA TAACCGCTGA AGAGTGCCTG
    AAGCAGTTCC CCCAGAGACT GAAGGAAGCC TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ATAGACACTG ACAGGGATGG CATAATCAAC ATGCATGATC TTCACAGATT GTTACTGCAC
    TTACTGTTCA ATCTTAAAGA CAAAGAATTT GAGCGTTTCC TGGGCCTTCT TGGCTTGAGA
    CTCAGCGTCA CTCTCAATTT TCGGGAATTT CGAAATCTGT GTGAGAAGAG AGCATTGAGA
    ACAGATGACG CGCCTCAAAG ACTAATCAGA CCAAAACAGA AGGTCGCAGA TTCAGAACTA
    GCTTGTGAAC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAACCAGATG GTCAGACTTA
    TCTAAGAATT TTATAGAAAC TGATAATGAG GGCAATGGCA TCCTTCGGCG GCGGGATATA
    AAGAATGCGC TCTATGGATT TGATATTCCT CTGACACCGA GGGAATTTGA AAAGCTCTGG
    ATGAGCTATG ACACCGAGGG AAGAGGGCAC ATAACTTACC AAGAATTTCT ACAGAAATTG
    GGGATTAACT ACTCAGCAGA CATTCATCGG CCCTATGCAG AGGATTACTT CAACTTCATG
    GGTCATTTCA CAAAACCCCA GCAGATGCAA GAGGAGGCCA GACAGCTAGA GCAGAGCGTG
    GACAGGACTG CGCCAGTTAG GGATAAGCTT AAGGACCATT ATCAAGATAT CAGCAAAGCA
    CTCACCAAAC TAGACAAATC CAAAAATGGC TACCTATCCA TGTGCAAGAT GCAGAAAGTG
    CTGCAGGAGC TCGGCTGCTC TCTCAAGGAG GAGGAGCTGA CCAATCTTCT GAACAGTTGG
    GAAATCAAAT GGCATGATAA CTCCATCAAT TACCTTGACT TCCTGAAAGC AGTGGAGAGC
    AGCAAGCCAA CAAGACCTCA GCCAAAGAAA AAAGAAGAGA GTGTGCCAAT CAGTTTCTCA
    ACACTGAACC CAGAGGAGGT TGTAAAGAAC ATCCAGGAAG TTGTTGCCTC CTCCGACATG
    GCTTTGTCAA CGGCATTTTC TGCATTGGAT AAAGAGGACA CTGGGTTTGT AAAGGCTTCA
    GATTTTGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CAGACAACCA GTATCATTAT
    TTTTTGAGAA AACTGAAAAT TCATCTAACC CCCCATATAA ATTGGAAATA TTTTCTCCAG
    AACTTCAGTT CTTTCTCTGA AGAGACTGCT GCCGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCACCGCCCA CATCTCCTCC CAAGGAAACA GCAAACCAGG AGCTGCTGGC TCAGCTCCAC
    AAGGCGGTGG GCTCCCACTA CCATGCGATT GCTCAGGAGT TTGACAACTT CGACACCATG
    AAAACGTACA CCGCCTCCAA GGATGAATTC AGGGCCATTT GTGCTCGCCA TGTCCAAATT
    CTGACAGATG AGCAATTTGA CAGACTGTGG AGCAAGATGC CAGTCAATGC CAAGGGGAGG
    CTGAAGTACC TGGACCTCCT GAGCATGTTC AGCTCCAAGA GAGCGCCAAC ACCATTGACC
    ACGGGCAGCT CGGCCAAGGC CCAGAGAGGC AGCAGTGTTC CTGAAATCTC AGATGGAAGC
    AGGCCTGCAG TATCATCGCC CATTCATGAC CTGAAGGCGG GGTTGAAAGC ACAGAGCCAC
    CCCTGTACCC CCGCTAGTGC CAGTTCGGCG CCAGGCACCC TGCCCTTGCA GAACTGCGAG
    CCCATAGAAA GCAAGCTCCA GAAGAAAATC CAGGGCTGCT GGCGAGAGTT CCTGAAAGAA
    TGCAAAGAGA AGGATGTGAA TAAGCAGGGC CAGGTCACCA CGGCTGAATT CCTGGCTCTT
    GTGGAGAAAT TCAATCTGGA CATAAGCAAA GAGGAATGTC AGCAGCTCAT CGTGAAGTAT
    GATTTAAAGA ACAACGGGAA GTTTGCTTAT TGCAACTTCA TCCAGAGCTG TGTCCTCCTG
    TTGAAAGCGA AGGAAACCTC ACTGATGCAG AGGATGAAAA TTCAGAATGC ACATAAAATG
    AAAGAATCTG GTGCTGAGAC TTCTTCCTTT TATTCAGCTT TACTGCGTAT CCAGCCCAAA
    ATTGTGCACT GCTGGAGGCC CATGCGGCGT ACCTTCAAAG CCTATGATGA GGGCGGGACA
    GGGCTTTTAA GCGTGGCTGA TTTCAGGAAG GTCCTGAGAC AGTACAGCAT CAACCTCTCG
    GAGGAGGAGT TCTTTCACAT TCTGGAGTAT TATGACAAGA CGCTGTCTTC CAAGATCTCC
    TACAACGACT TCCTGCGGGC GTTCCTTCAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280039.1
    CDS33..4385
    Translation

    Target ORF information:

    RefSeq Version XM_022424331.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424331.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATTTTC
    AGAAACATTA AAACTATTTT CAAAGCCTTC AAGCTCATTG ATGTTAGCAA GACTGGTCTG
    ATCCAGTCTT GTGAACTGAG GAAAGTTCTC GAGACCTTCT GTTTGAAGAT GAAAGATGAT
    GAATACAAAA AGTTTGCAAA ACACTACAAC ATTGATAAAG ATACTGCAGT GGATTACAAT
    GTTTTTTTGA AGAACCTCAG TGCAAATAAT GACTTGAACC TTAGATATTA TATGGGAAAC
    CAAGATATCT CCTGGAAGAA TCAACCAGTC CAAAATTCCA AAGGGGAGTG CCTCCACAGT
    TCTGGTTCAT CTGAAGATGT CTGGAAAAAC TACAACCTAG ACGAAATTGA GAGAACTTTT
    TGCCAAGAGC TTCCCAAGTG CTATGAAAAG GTTGAAAAAG CCCTGAGTGC AGGGGACCCC
    TCCAAAAGTG GATATGTATC TTTGAATTAC CTAAAGATTG TCCTCGACAC CTTTGTATAC
    CGATTACCAA GGAGAATTTT TATCCAGTTA ATGAAAAGAT TTGGACTTAA AACCACCACC
    AAAATCAATT GGAAACAATT TCTAACATCA CTTTATGAAC CCCAGTGGTT GGAAGTCAAC
    AATACAGTTC CTCCAACAAA AAGAAATAGA ATCACTTCTA GAGATCAATC TTGCAAGGAA
    AACATTATCA CAAAGTTATC TAGACAAGAG GATCACTATA CATCATTGAA GAAAGCACTC
    CTGATCGCCA ACACTAAACC TGATGGACAG ATAACGGGGA AAGAATTGCG ATATATTCTA
    AATTGCATGG TTGTAAAAAT CAGTGATTCA GAATTCAAAG AGCTCATGCA AATGCTTGAC
    CCTGGGGACA CCGGATGGGT TAACATCTAC ACACTTATTG AACTGCTTGA AGAGACCCCT
    AAGATACAAA AACTCTCCTC CTCTGCAGAC ACCAAGGCGC CTCTCCTCCT GGCTTGGGAT
    TCAGTGGAAG AAATCATTCA GGACTCAATT GCTAGGAATC TCCAAGCCTT CTACAAAATG
    CTACGGTCAT ATGACCTCGG AGACACAGGG CTCATTGGTC GAAATAATTT CAAGAAAATC
    ATGCGCATCT TCTGTCCATT TTTAACAATC GAACATTTAC TGAAACTCTG TAGTAGGTTT
    CAGGATATTG CTTCAGGAAG AATCCTTTAT AAGAAATTTT TGGCATCCAT GGGGATGAGT
    GGCCCACCAC CCCCCTCTCC GGTTATTACT CCAAAGGATC AGCTATTGAG TGACCATTTT
    CAAAGAGAGG AGCAGCGGAA GTTGGATCTT TCTGAAAGAA CCAAGCCCGT GGAGGACAGA
    AGCACCTCGA CCAAGAAAAT GACGAAGGAT GAAGCGATCG AAAAACTAAA AAACTGTATT
    CAGCAGCGGG ACCCAGCATT CAAAAAATTC TTTCTTGACT ATAGCAAGGA GCTCACTGGC
    AAAATTCATG TGCGTGACTT CAAGAAGGTA CTGGAAGCTA GTGGAATGCC CATGGATGAT
    GACCAGTACG CTCTGCTGAC GGCAAAAATA GGATATAAGA GAGAAGGGAT GAGTTATCTT
    GATTTTGCAG CAGGATTTGA AGATGCTAAA ATGAGCAGGC CAGAAGTGAT CCCATTGCAG
    ACTCCAGTTC TTTCCAAGAC TAATTTGGAC AGATACTTTA TAACCGCTGA AGAGTGCCTG
    AAGCAGTTCC CCCAGAGACT GAAGGAAGCC TTCCGGGACC CCTACTCTGC CTTCTTTAAA
    ATAGACACTG ACAGGGATGG CATAATCAAC ATGCATGATC TTCACAGATT GTTACTGCAC
    TTACTGTTCA ATCTTAAAGA CAAAGAATTT GAGCGTTTCC TGGGCCTTCT TGGCTTGAGA
    CTCAGCGTCA CTCTCAATTT TCGGGAATTT CGAAATCTGT GTGAGAAGAG AGCATTGAGA
    ACAGATGACG CGCCTCAAAG ACTAATCAGA CCAAAACAGA AGGTCGCAGA TTCAGAACTA
    GCTTGTGAAC AGGCTCATCA GTATCTTGTT ACCAAAGCAA AAACCAGATG GTCAGACTTA
    TCTAAGAATT TTATAGAAAC TGATAATGAG GGCAATGGCA TCCTTCGGCG GCGGGATATA
    AAGAATGCGC TCTATGGATT TGATATTCCT CTGACACCGA GGGAATTTGA AAAGCTCTGG
    ATGAGCTATG ACACCGAGGG AAGAGGGCAC ATAACTTACC AAGAATTTCT ACAGAAATTG
    GGGATTAACT ACTCAGCAGA CATTCATCGG CCCTATGCAG AGGATTACTT CAACTTCATG
    GGTCATTTCA CAAAACCCCA GCAGATGCAA GAGGAGGCCA GACAGCTAGA GCAGAGCGTG
    GACAGGACTG CGCCAGTTAG GGATAAGCTT AAGGACCATT ATCAAGATAT CAGCAAAGCA
    CTCACCAAAC TAGACAAATC CAAAAATGGC TACCTATCCA TGTGCAAGAT GCAGAAAGTG
    CTGCAGGAGC TCGGCTGCTC TCTCAAGGAG GAGGAGCTGA CCAATCTTCT GAACAGTTGG
    GAAATCAAAT GGCATGATAA CTCCATCAAT TACCTTGACT TCCTGAAAGC AGTGGAGAGC
    AGCAAGCCAA CAAGACCTCA GCCAAAGAAA AAAGAAGAGA GTGTGCCAAT CAGTTTCTCA
    ACACTGAACC CAGAGGAGGT TGTAAAGAAC ATCCAGGAAG TTGTTGCCTC CTCCGACATG
    GCTTTGTCAA CGGCATTTTC TGCATTGGAT AAAGAGGACA CTGGGTTTGT AAAGGCTTCA
    GATTTTGGAC AAGTTCTTAA GGATTTCTGT TACAAACTAA CAGACAACCA GTATCATTAT
    TTTTTGAGAA AACTGAAAAT TCATCTAACC CCCCATATAA ATTGGAAATA TTTTCTCCAG
    AACTTCAGTT CTTTCTCTGA AGAGACTGCT GCCGAGTGGG CTGAGAAAAT GCCCAAAGGC
    CCACCGCCCA CATCTCCTCC CAAGGAAACA GCAAACCAGG AGCTGCTGGC TCAGCTCCAC
    AAGGCGGTGG GCTCCCACTA CCATGCGATT GCTCAGGAGT TTGACAACTT CGACACCATG
    AAAACGTACA CCGCCTCCAA GGATGAATTC AGGGCCATTT GTGCTCGCCA TGTCCAAATT
    CTGACAGATG AGCAATTTGA CAGACTGTGG AGCAAGATGC CAGTCAATGC CAAGGGGAGG
    CTGAAGTACC TGGACCTCCT GAGCATGTTC AGCTCCAAGA GAGCGCCAAC ACCATTGACC
    ACGGGCAGCT CGGCCAAGGC CCAGAGAGGC AGCAGTGTTC CTGAAATCTC AGATGGAAGC
    AGGCCTGCAG TATCATCGCC CATTCATGAC CTGAAGGCGG GGTTGAAAGC ACAGAGCCAC
    CCCTGTACCC CCGCTAGTGC CAGTTCGGCG CCAGGCACCC TGCCCTTGCA GAACTGCGAG
    CCCATAGAAA GCAAGCTCCA GAAGAAAATC CAGGGCTGCT GGCGAGAGTT CCTGAAAGAA
    TGCAAAGAGA AGGATGTGAA TAAGCAGGGC CAGGTCACCA CGGCTGAATT CCTGGCTCTT
    GTGGAGAAAT TCAATCTGGA CATAAGCAAA GAGGAATGTC AGCAGCTCAT CGTGAAGTAT
    GATTTAAAGA ACAACGGGAA GTTTGCTTAT TGCAACTTCA TCCAGAGCTG TGTCCTCCTG
    TTGAAAGCGA AGGAAACCTC ACTGATGCAG AGGATGAAAA TTCAGAATGC ACATAAAATG
    AAAGAATCTG GTGCTGAGAC TTCTTCCTTT TATTCAGCTT TACTGCGTAT CCAGCCCAAA
    ATTGTGCACT GCTGGAGGCC CATGCGGCGT ACCTTCAAAG CCTATGATGA GGGCGGGACA
    GGGCTTTTAA GCGTGGCTGA TTTCAGGAAG GTCCTGAGAC AGTACAGCAT CAACCTCTCG
    GAGGAGGAGT TCTTTCACAT TCTGGAGTAT TATGACAAGA CGCTGTCTTC CAAGATCTCC
    TACAACGACT TCCTGCGGGC GTTCCTTCAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe291395
    Clone ID Related Accession (Same CDS sequence) XM_022424332.1
    Accession Version XM_022424332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAACAGA ATAGCACCAA AACACACGTT TCCACAATCA ACAGCACCAA ATCCTGCAGT 
    ACCAAGGCCA GGGATTTAAT GTGCAAAATG GCAATTATAC CAGATTGGCA AACTTCGTAT
    CCTCACACAG GAAAGCTCAA GAATTTAAGA CCTCATTCTT CACCATGTAG AGTGTATTCA
    AGGAACAGTT TCCAAAATAT GTTCAGACCT TCTTCTTCAA CCACAGCTAT TGCAAATCCG
    ATACTGTCCT TCTTAGATGT TAAACGGCTT TTATTTCAAA AAATCACTGA TAAAGGATAT
    GCGTTGAAAA AGGCTTTTGA GCTGCTTGAC ACCAGTCACA ACATGACGGT ATCAAAGAAT
    GAACTGAGAA GAATTATCAC AACCTTCCTG ATGCCTCTCA CGAGAGAACA GTTTCAGGAT
    GTACTGGCTC AGATCCCCCT CACCAGCTCT GGGGCCATTC CATACCTTGA ATTCCTGTCC
    TGGTTTGGTG GAATGGACCT AAGTATAAAT GTTATACAGA GGGGAGGTGG GAATGAAATG
    AGTTGCAGTC GGACAGTAAA AGAACTTGAA AAACAAATAG GAGAAAAGAT TTTCAGAAAC
    ATTAAAACTA TTTTCAAAGC CTTCAAGCTC ATTGATGTTA GCAAGACTGG TCTGATCCAG
    TCTTGTGAAC TGAGGAAAGT TCTCGAGACC TTCTGTTTGA AGATGAAAGA TGATGAATAC
    AAAAAGTTTG CAAAACACTA CAACATTGAT AAAGATACTG CAGTGGATTA CAATGTTTTT
    TTGAAGAACC TCAGTGCAAA TAATGACTTG AACCTTAGAT ATTATATGGG AAACCAAGAT
    ATCTCCTGGA AGAATCAACC AGTCCAAAAT TCCAAAGGGG AGTGCCTCCA CAGTTCTGGT
    TCATCTGAAG ATGTCTGGAA AAACTACAAC CTAGACGAAA TTGAGAGAAC TTTTTGCCAA
    GAGCTTCCCA AGTGCTATGA AAAGGTTGAA AAAGCCCTGA GTGCAGGGGA CCCCTCCAAA
    AGTGGATATG TATCTTTGAA TTACCTAAAG ATTGTCCTCG ACACCTTTGT ATACCGATTA
    CCAAGGAGAA TTTTTATCCA GTTAATGAAA AGATTTGGAC TTAAAACCAC CACCAAAATC
    AATTGGAAAC AATTTCTAAC ATCACTTTAT GAACCCCAGT GGTTGGAAGT CAACAATACA
    GTTCCTCCAA CAAAAAGAAA TAGAATCACT TCTAGAGATC AATCTTGCAA GGAAAACATT
    ATCACAAAGT TATCTAGACA AGAGGATCAC TATACATCAT TGAAGAAAGC ACTCCTGATC
    GCCAACACTA AACCTGATGG ACAGATAACG GGGAAAGAAT TGCGATATAT TCTAAATTGC
    ATGGTTGTAA AAATCAGTGA TTCAGAATTC AAAGAGCTCA TGCAAATGCT TGACCCTGGG
    GACACCGGAT GGGTTAACAT CTACACACTT ATTGAACTGC TTGAAGAGAC CCCTAAGATA
    CAAAAACTCT CCTCCTCTGC AGACACCAAG GCGCCTCTCC TCCTGGCTTG GGATTCAGTG
    GAAGAAATCA TTCAGGACTC AATTGCTAGG AATCTCCAAG CCTTCTACAA AATGCTACGG
    TCATATGACC TCGGAGACAC AGGGCTCATT GGTCGAAATA ATTTCAAGAA AATCATGCGC
    ATCTTCTGTC CATTTTTAAC AATCGAACAT TTACTGAAAC TCTGTAGTAG GTTTCAGGAT
    ATTGCTTCAG GAAGAATCCT TTATAAGAAA TTTTTGGCAT CCATGGGGAT GAGTGGCCCA
    CCACCCCCCT CTCCGGTTAT TACTCCAAAG GATCAGCTAT TGAGTGACCA TTTTCAAAGA
    GAGGAGCAGC GGAAGTTGGA TCTTTCTGAA AGAACCAAGC CCGTGGAGGA CAGAAGCACC
    TCGACCAAGA AAATGACGAA GGATGAAGCG ATCGAAAAAC TAAAAAACTG TATTCAGCAG
    CGGGACCCAG CATTCAAAAA ATTCTTTCTT GACTATAGCA AGGAGCTCAC TGGCAAAATT
    CATGTGCGTG ACTTCAAGAA GGTACTGGAA GCTAGTGGAA TGCCCATGGA TGATGACCAG
    TACGCTCTGC TGACGGCAAA AATAGGATAT AAGAGAGAAG GGATGAGTTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATGC TAAAATGAGC AGGCCAGAAG TGATCCCATT GCAGACTCCA
    GTTCTTTCCA AGACTAATTT GGACAGATAC TTTATAACCG CTGAAGAGTG CCTGAAGCAG
    TTCCCCCAGA GACTGAAGGA AGCCTTCCGG GACCCCTACT CTGCCTTCTT TAAAATAGAC
    ACTGACAGGG ATGGCATAAT CAACATGCAT GATCTTCACA GATTGTTACT GCACTTACTG
    TTCAATCTTA AAGACAAAGA ATTTGAGCGT TTCCTGGGCC TTCTTGGCTT GAGACTCAGC
    GTCACTCTCA ATTTTCGGGA ATTTCGAAAT CTGTGTGAGA AGAGAGCATT GAGAACAGAT
    GACGCGCCTC AAAGACTAAT CAGACCAAAA CAGAAGGTCG CAGATTCAGA ACTAGCTTGT
    GAACAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAACCA GATGGTCAGA CTTATCTAAG
    AATTTTATAG AAACTGATAA TGAGGGCAAT GGCATCCTTC GGCGGCGGGA TATAAAGAAT
    GCGCTCTATG GATTTGATAT TCCTCTGACA CCGAGGGAAT TTGAAAAGCT CTGGATGAGC
    TATGACACCG AGGGAAGAGG GCACATAACT TACCAAGAAT TTCTACAGAA ATTGGGGATT
    AACTACTCAG CAGACATTCA TCGGCCCTAT GCAGAGGATT ACTTCAACTT CATGGGTCAT
    TTCACAAAAC CCCAGCAGAT GCAAGAGGAG GCCAGACAGC TAGAGCAGAG CGTGGACAGG
    ACTGCGCCAG TTAGGGATAA GCTTAAGGAC CATTATCAAG ATATCAGCAA AGCACTCACC
    AAACTAGACA AATCCAAAAA TGGCTACCTA TCCATGTGCA AGATGCAGAA AGTGCTGCAG
    GAGCTCGGCT GCTCTCTCAA GGAGGAGGAG CTGACCAATC TTCTGAACAG TTGGGAAATC
    AAATGGCATG ATAACTCCAT CAATTACCTT GACTTCCTGA AAGCAGTGGA GAGCAGCAAG
    CCAACAAGAC CTCAGCCAAA GAAAAAAGAA GAGAGTGTGC CAATCAGTTT CTCAACACTG
    AACCCAGAGG AGGTTGTAAA GAACATCCAG GAAGTTGTTG CCTCCTCCGA CATGGCTTTG
    TCAACGGCAT TTTCTGCATT GGATAAAGAG GACACTGGGT TTGTAAAGGC TTCAGATTTT
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACAGACA ACCAGTATCA TTATTTTTTG
    AGAAAACTGA AAATTCATCT AACCCCCCAT ATAAATTGGA AATATTTTCT CCAGAACTTC
    AGTTCTTTCT CTGAAGAGAC TGCTGCCGAG TGGGCTGAGA AAATGCCCAA AGGCCCACCG
    CCCACATCTC CTCCCAAGGA AACAGCAAAC CAGGAGCTGC TGGCTCAGCT CCACAAGGCG
    GTGGGCTCCC ACTACCATGC GATTGCTCAG GAGTTTGACA ACTTCGACAC CATGAAAACG
    TACACCGCCT CCAAGGATGA ATTCAGGGCC ATTTGTGCTC GCCATGTCCA AATTCTGACA
    GATGAGCAAA CCCCCGCTAG TGCCAGTTCG GCGCCAGGCA CCCTGCCCTT GCAGAACTGC
    GAGCCCATAG AAAGCAAGCT CCAGAAGAAA ATCCAGGGCT GCTGGCGAGA GTTCCTGAAA
    GAATGCAAAG AGAAGGATGT GAATAAGCAG GGCCAGGTCA CCACGGCTGA ATTCCTGGCT
    CTTGTGGAGA AATTCAATCT GGACATAAGC AAAGAGGAAT GTCAGCAGCT CATCGTGAAG
    TATGATTTAA AGAACAACGG GAAGTTTGCT TATTGCAACT TCATCCAGAG CTGTGTCCTC
    CTGTTGAAAG CGAAGGAAAC CTCACTGATG CAGAGGATGA AAATTCAGAA TGCACATAAA
    ATGAAAGAAT CTGGTGCTGA GACTTCTTCC TTTTATTCAG CTTTACTGCG TATCCAGCCC
    AAAATTGTGC ACTGCTGGAG GCCCATGCGG CGTACCTTCA AAGCCTATGA TGAGGGCGGG
    ACAGGGCTTT TAAGCGTGGC TGATTTCAGG AAGGTCCTGA GACAGTACAG CATCAACCTC
    TCGGAGGAGG AGTTCTTTCA CATTCTGGAG TATTATGACA AGACGCTGTC TTCCAAGATC
    TCCTACAACG ACTTCCTGCG GGCGTTCCTT CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280040.1
    CDS333..4688
    Translation

    Target ORF information:

    RefSeq Version XM_022424332.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAACAGA ATAGCACCAA AACACACGTT TCCACAATCA ACAGCACCAA ATCCTGCAGT 
    ACCAAGGCCA GGGATTTAAT GTGCAAAATG GCAATTATAC CAGATTGGCA AACTTCGTAT
    CCTCACACAG GAAAGCTCAA GAATTTAAGA CCTCATTCTT CACCATGTAG AGTGTATTCA
    AGGAACAGTT TCCAAAATAT GTTCAGACCT TCTTCTTCAA CCACAGCTAT TGCAAATCCG
    ATACTGTCCT TCTTAGATGT TAAACGGCTT TTATTTCAAA AAATCACTGA TAAAGGATAT
    GCGTTGAAAA AGGCTTTTGA GCTGCTTGAC ACCAGTCACA ACATGACGGT ATCAAAGAAT
    GAACTGAGAA GAATTATCAC AACCTTCCTG ATGCCTCTCA CGAGAGAACA GTTTCAGGAT
    GTACTGGCTC AGATCCCCCT CACCAGCTCT GGGGCCATTC CATACCTTGA ATTCCTGTCC
    TGGTTTGGTG GAATGGACCT AAGTATAAAT GTTATACAGA GGGGAGGTGG GAATGAAATG
    AGTTGCAGTC GGACAGTAAA AGAACTTGAA AAACAAATAG GAGAAAAGAT TTTCAGAAAC
    ATTAAAACTA TTTTCAAAGC CTTCAAGCTC ATTGATGTTA GCAAGACTGG TCTGATCCAG
    TCTTGTGAAC TGAGGAAAGT TCTCGAGACC TTCTGTTTGA AGATGAAAGA TGATGAATAC
    AAAAAGTTTG CAAAACACTA CAACATTGAT AAAGATACTG CAGTGGATTA CAATGTTTTT
    TTGAAGAACC TCAGTGCAAA TAATGACTTG AACCTTAGAT ATTATATGGG AAACCAAGAT
    ATCTCCTGGA AGAATCAACC AGTCCAAAAT TCCAAAGGGG AGTGCCTCCA CAGTTCTGGT
    TCATCTGAAG ATGTCTGGAA AAACTACAAC CTAGACGAAA TTGAGAGAAC TTTTTGCCAA
    GAGCTTCCCA AGTGCTATGA AAAGGTTGAA AAAGCCCTGA GTGCAGGGGA CCCCTCCAAA
    AGTGGATATG TATCTTTGAA TTACCTAAAG ATTGTCCTCG ACACCTTTGT ATACCGATTA
    CCAAGGAGAA TTTTTATCCA GTTAATGAAA AGATTTGGAC TTAAAACCAC CACCAAAATC
    AATTGGAAAC AATTTCTAAC ATCACTTTAT GAACCCCAGT GGTTGGAAGT CAACAATACA
    GTTCCTCCAA CAAAAAGAAA TAGAATCACT TCTAGAGATC AATCTTGCAA GGAAAACATT
    ATCACAAAGT TATCTAGACA AGAGGATCAC TATACATCAT TGAAGAAAGC ACTCCTGATC
    GCCAACACTA AACCTGATGG ACAGATAACG GGGAAAGAAT TGCGATATAT TCTAAATTGC
    ATGGTTGTAA AAATCAGTGA TTCAGAATTC AAAGAGCTCA TGCAAATGCT TGACCCTGGG
    GACACCGGAT GGGTTAACAT CTACACACTT ATTGAACTGC TTGAAGAGAC CCCTAAGATA
    CAAAAACTCT CCTCCTCTGC AGACACCAAG GCGCCTCTCC TCCTGGCTTG GGATTCAGTG
    GAAGAAATCA TTCAGGACTC AATTGCTAGG AATCTCCAAG CCTTCTACAA AATGCTACGG
    TCATATGACC TCGGAGACAC AGGGCTCATT GGTCGAAATA ATTTCAAGAA AATCATGCGC
    ATCTTCTGTC CATTTTTAAC AATCGAACAT TTACTGAAAC TCTGTAGTAG GTTTCAGGAT
    ATTGCTTCAG GAAGAATCCT TTATAAGAAA TTTTTGGCAT CCATGGGGAT GAGTGGCCCA
    CCACCCCCCT CTCCGGTTAT TACTCCAAAG GATCAGCTAT TGAGTGACCA TTTTCAAAGA
    GAGGAGCAGC GGAAGTTGGA TCTTTCTGAA AGAACCAAGC CCGTGGAGGA CAGAAGCACC
    TCGACCAAGA AAATGACGAA GGATGAAGCG ATCGAAAAAC TAAAAAACTG TATTCAGCAG
    CGGGACCCAG CATTCAAAAA ATTCTTTCTT GACTATAGCA AGGAGCTCAC TGGCAAAATT
    CATGTGCGTG ACTTCAAGAA GGTACTGGAA GCTAGTGGAA TGCCCATGGA TGATGACCAG
    TACGCTCTGC TGACGGCAAA AATAGGATAT AAGAGAGAAG GGATGAGTTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATGC TAAAATGAGC AGGCCAGAAG TGATCCCATT GCAGACTCCA
    GTTCTTTCCA AGACTAATTT GGACAGATAC TTTATAACCG CTGAAGAGTG CCTGAAGCAG
    TTCCCCCAGA GACTGAAGGA AGCCTTCCGG GACCCCTACT CTGCCTTCTT TAAAATAGAC
    ACTGACAGGG ATGGCATAAT CAACATGCAT GATCTTCACA GATTGTTACT GCACTTACTG
    TTCAATCTTA AAGACAAAGA ATTTGAGCGT TTCCTGGGCC TTCTTGGCTT GAGACTCAGC
    GTCACTCTCA ATTTTCGGGA ATTTCGAAAT CTGTGTGAGA AGAGAGCATT GAGAACAGAT
    GACGCGCCTC AAAGACTAAT CAGACCAAAA CAGAAGGTCG CAGATTCAGA ACTAGCTTGT
    GAACAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAACCA GATGGTCAGA CTTATCTAAG
    AATTTTATAG AAACTGATAA TGAGGGCAAT GGCATCCTTC GGCGGCGGGA TATAAAGAAT
    GCGCTCTATG GATTTGATAT TCCTCTGACA CCGAGGGAAT TTGAAAAGCT CTGGATGAGC
    TATGACACCG AGGGAAGAGG GCACATAACT TACCAAGAAT TTCTACAGAA ATTGGGGATT
    AACTACTCAG CAGACATTCA TCGGCCCTAT GCAGAGGATT ACTTCAACTT CATGGGTCAT
    TTCACAAAAC CCCAGCAGAT GCAAGAGGAG GCCAGACAGC TAGAGCAGAG CGTGGACAGG
    ACTGCGCCAG TTAGGGATAA GCTTAAGGAC CATTATCAAG ATATCAGCAA AGCACTCACC
    AAACTAGACA AATCCAAAAA TGGCTACCTA TCCATGTGCA AGATGCAGAA AGTGCTGCAG
    GAGCTCGGCT GCTCTCTCAA GGAGGAGGAG CTGACCAATC TTCTGAACAG TTGGGAAATC
    AAATGGCATG ATAACTCCAT CAATTACCTT GACTTCCTGA AAGCAGTGGA GAGCAGCAAG
    CCAACAAGAC CTCAGCCAAA GAAAAAAGAA GAGAGTGTGC CAATCAGTTT CTCAACACTG
    AACCCAGAGG AGGTTGTAAA GAACATCCAG GAAGTTGTTG CCTCCTCCGA CATGGCTTTG
    TCAACGGCAT TTTCTGCATT GGATAAAGAG GACACTGGGT TTGTAAAGGC TTCAGATTTT
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACAGACA ACCAGTATCA TTATTTTTTG
    AGAAAACTGA AAATTCATCT AACCCCCCAT ATAAATTGGA AATATTTTCT CCAGAACTTC
    AGTTCTTTCT CTGAAGAGAC TGCTGCCGAG TGGGCTGAGA AAATGCCCAA AGGCCCACCG
    CCCACATCTC CTCCCAAGGA AACAGCAAAC CAGGAGCTGC TGGCTCAGCT CCACAAGGCG
    GTGGGCTCCC ACTACCATGC GATTGCTCAG GAGTTTGACA ACTTCGACAC CATGAAAACG
    TACACCGCCT CCAAGGATGA ATTCAGGGCC ATTTGTGCTC GCCATGTCCA AATTCTGACA
    GATGAGCAAA CCCCCGCTAG TGCCAGTTCG GCGCCAGGCA CCCTGCCCTT GCAGAACTGC
    GAGCCCATAG AAAGCAAGCT CCAGAAGAAA ATCCAGGGCT GCTGGCGAGA GTTCCTGAAA
    GAATGCAAAG AGAAGGATGT GAATAAGCAG GGCCAGGTCA CCACGGCTGA ATTCCTGGCT
    CTTGTGGAGA AATTCAATCT GGACATAAGC AAAGAGGAAT GTCAGCAGCT CATCGTGAAG
    TATGATTTAA AGAACAACGG GAAGTTTGCT TATTGCAACT TCATCCAGAG CTGTGTCCTC
    CTGTTGAAAG CGAAGGAAAC CTCACTGATG CAGAGGATGA AAATTCAGAA TGCACATAAA
    ATGAAAGAAT CTGGTGCTGA GACTTCTTCC TTTTATTCAG CTTTACTGCG TATCCAGCCC
    AAAATTGTGC ACTGCTGGAG GCCCATGCGG CGTACCTTCA AAGCCTATGA TGAGGGCGGG
    ACAGGGCTTT TAAGCGTGGC TGATTTCAGG AAGGTCCTGA GACAGTACAG CATCAACCTC
    TCGGAGGAGG AGTTCTTTCA CATTCTGGAG TATTATGACA AGACGCTGTC TTCCAAGATC
    TCCTACAACG ACTTCCTGCG GGCGTTCCTT CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38866
    Clone ID Related Accession (Same CDS sequence) XM_014117294.1 , XM_014117294.2
    Accession Version XM_014117294.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAACAGA ATAGCACCAA AACACACGTT TCCACAATCA ACAGCACCAA ATCCTGCAGT 
    ACCAAGGCCA GGGATTTAAT GTGCAAAATG GCAATTATAC CAGATTGGCA AACTTCGTAT
    CCTCACACAG GAAAGCTCAA GAATTTAAGA CCTCATTCTT CACCATGTAG AGTGTATTCA
    AGGAACAGTT TCCAAAATAT GTTCAGACCT TCTTCTTCAA CCACAGCTAT TGCAAATCCG
    ATACTGTCCT TCTTAGATGT TAAACGGCTT TTATTTCAAA AAATCACTGA TAAAGGATAT
    GCGTTGAAAA AGGCTTTTGA GCTGCTTGAC ACCAGTCACA ACATGACGGT ATCAAAGAAT
    GAACTGAGAA GAATTATCAC AACCTTCCTG ATGCCTCTCA CGAGAGAACA GTTTCAGGAT
    GTACTGGCTC AGATCCCCCT CACCAGCTCT GGGGCCATTC CATACCTTGA ATTCCTGTCC
    TGGTTTGGTG GAATGGACCT AAGTATAAAT GTTATACAGA GGGGAGGTGG GAATGAAATG
    AGTTGCAGTC GGACAGTAAA AGAACTTGAA AAACAAATAG GAGAAAAGAT TTTCAGAAAC
    ATTAAAACTA TTTTCAAAGC CTTCAAGCTC ATTGATGTTA GCAAGACTGG TCTGATCCAG
    TCTTGTGAAC TGAGGAAAGT TCTCGAGACC TTCTGTTTGA AGATGAAAGA TGATGAATAC
    AAAAAGTTTG CAAAACACTA CAACATTGAT AAAGATACTG CAGTGGATTA CAATGTTTTT
    TTGAAGAACC TCAGTGCAAA TAATGACTTG AACCTTAGAT ATTATATGGG AAACCAAGAT
    ATCTCCTGGA AGAATCAACC AGTCCAAAAT TCCAAAGGGG AGTGCCTCCA CAGTTCTGGT
    TCATCTGAAG ATGTCTGGAA AAACTACAAC CTAGACGAAA TTGAGAGAAC TTTTTGCCAA
    GAGCTTCCCA AGTGCTATGA AAAGGTTGAA AAAGCCCTGA GTGCAGGGGA CCCCTCCAAA
    AGTGGATATG TATCTTTGAA TTACCTAAAG ATTGTCCTCG ACACCTTTGT ATACCGATTA
    CCAAGGAGAA TTTTTATCCA GTTAATGAAA AGATTTGGAC TTAAAACCAC CACCAAAATC
    AATTGGAAAC AATTTCTAAC ATCACTTTAT GAACCCCAGT GGTTGGAAGT CAACAATACA
    GTTCCTCCAA CAAAAAGAAA TAGAATCACT TCTAGAGATC AATCTTGCAA GGAAAACATT
    ATCACAAAGT TATCTAGACA AGAGGATCAC TATACATCAT TGAAGAAAGC ACTCCTGATC
    GCCAACACTA AACCTGATGG ACAGATAACG GGGAAAGAAT TGCGATATAT TCTAAATTGC
    ATGGTTGTAA AAATCAGTGA TTCAGAATTC AAAGAGCTCA TGCAAATGCT TGACCCTGGG
    GACACCGGAT GGGTTAACAT CTACACACTT ATTGAACTGC TTGAAGAGAC CCCTAAGATA
    CAAAAACTCT CCTCCTCTGC AGACACCAAG GCGCCTCTCC TCCTGGCTTG GGATTCAGTG
    GAAGAAATCA TTCAGGACTC AATTGCTAGG AATCTCCAAG CCTTCTACAA AATGCTACGG
    TCATATGACC TCGGAGACAC AGGGCTCATT GGTCGAAATA ATTTCAAGAA AATCATGCGC
    ATCTTCTGTC CATTTTTAAC AATCGAACAT TTACTGAAAC TCTGTAGTAG GTTTCAGGAT
    ATTGCTTCAG GAAGAATCCT TTATAAGAAA TTTTTGGCAT CCATGGGGAT GAGTGGCCCA
    CCACCCCCCT CTCCGGTTAT TACTCCAAAG GATCAGCTAT TGAGTGACCA TTTTCAAAGA
    GAGGAGCAGC GGAAGTTGGA TCTTTCTGAA AGAACCAAGC CCGTGGAGGA CAGAAGCACC
    TCGACCAAGA AAATGACGAA GGATGAAGCG ATCGAAAAAC TAAAAAACTG TATTCAGCAG
    CGGGACCCAG CATTCAAAAA ATTCTTTCTT GACTATAGCA AGGAGCTCAC TGGCAAAATT
    CATGTGCGTG ACTTCAAGAA GGTACTGGAA GCTAGTGGAA TGCCCATGGA TGATGACCAG
    TACGCTCTGC TGACGGCAAA AATAGGATAT AAGAGAGAAG GGATGAGTTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATGC TAAAATGAGC AGGCCAGAAG TGATCCCATT GCAGACTCCA
    GTTCTTTCCA AGACTAATTT GGACAGATAC TTTATAACCG CTGAAGAGTG CCTGAAGCAG
    TTCCCCCAGA GACTGAAGGA AGCCTTCCGG GACCCCTACT CTGCCTTCTT TAAAATAGAC
    ACTGACAGGG ATGGCATAAT CAACATGCAT GATCTTCACA GATTGTTACT GCACTTACTG
    TTCAATCTTA AAGACAAAGA ATTTGAGCGT TTCCTGGGCC TTCTTGGCTT GAGACTCAGC
    GTCACTCTCA ATTTTCGGGA ATTTCGAAAT CTGTGTGAGA AGAGAGCATT GAGAACAGAT
    GACGCGCCTC AAAGACTAAT CAGACCAAAA CAGAAGGTCG CAGATTCAGA ACTAGCTTGT
    GAACAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAACCA GATGGTCAGA CTTATCTAAG
    AATTTTATAG AAACTGATAA TGAGGGCAAT GGCATCCTTC GGCGGCGGGA TATAAAGAAT
    GCGCTCTATG GATTTGATAT TCCTCTGACA CCGAGGGAAT TTGAAAAGCT CTGGATGAGC
    TATGACACCG AGGGAAGAGG GCACATAACT TACCAAGAAT TTCTACAGAA ATTGGGGATT
    AACTACTCAG CAGACATTCA TCGGCCCTAT GCAGAGGATT ACTTCAACTT CATGGGTCAT
    TTCACAAAAC CCCAGCAGAT GCAAGAGGAG GCCAGACAGC TAGAGCAGAG CGTGGACAGG
    ACTGCGCCAG TTAGGGATAA GCTTAAGGAC CATTATCAAG ATATCAGCAA AGCACTCACC
    AAACTAGACA AATCCAAAAA TGGCTACCTA TCCATGTGCA AGATGCAGAA AGTGCTGCAG
    GAGCTCGGCT GCTCTCTCAA GGAGGAGGAG CTGACCAATC TTCTGAACAG TTGGGAAATC
    AAATGGCATG ATAACTCCAT CAATTACCTT GACTTCCTGA AAGCAGTGGA GAGCAGCAAG
    CCAACAAGAC CTCAGCCAAA GAAAAAAGAA GAGAGTGTGC CAATCAGTTT CTCAACACTG
    AACCCAGAGG AGGTTGTAAA GAACATCCAG GAAGTTGTTG CCTCCTCCGA CATGGCTTTG
    TCAACGGCAT TTTCTGCATT GGATAAAGAG GACACTGGGT TTGTAAAGGC TTCAGATTTT
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACAGACA ACCAGTATCA TTATTTTTTG
    AGAAAACTGA AAATTCATCT AACCCCCCAT ATAAATTGGA AATATTTTCT CCAGAACTTC
    AGTTCTTTCT CTGAAGAGAC TGCTGCCGAG TGGGCTGAGA AAATGCCCAA AGGCCCACCG
    CCCACATCTC CTCCCAAGGA AACAGCAAAC CAGGAGCTGC TGGCTCAGCT CCACAAGGCG
    GTGGGCTCCC ACTACCATGC GATTGCTCAG GAGTTTGACA ACTTCGACAC CATGAAAACG
    TACACCGCCT CCAAGGATGA ATTCAGGGCC ATTTGTGCTC GCCATGTCCA AATTCTGACA
    GATGAGCAAT TTGACAGACT GTGGAGCAAG ATGCCAGTCA ATGCCAAGGG GAGGCTGAAG
    TACCTGGACC TCCTGAGCAT GTTCAGCTCC AAGAGAGCGC CAACACCATT GACCACGGGC
    AGCTCGGCCA AGGCCCAGAG AGGCAGCAGT GTTCCTGAAA TCTCAGATGG AAGCAGGCCT
    GCAGTATCAT CGCCCATTCA TGACCTGAAG GCGGGGTTGA AAGCACAGAG CCACCCCTGT
    ACCCCCGCTA GTGCCAGTTC GGCGCCAGGC ACCCTGCCCT TGCAGAACTG CGAGCCCATA
    GAAAGCAAGC TCCAGAAGAA AATCCAGGGC TGCTGGCGAG AGTTCCTGAA AGAATGCAAA
    GAGAAGGATG TGAATAAGCA GGGCCAGGTC ACCACGGCTG AATTCCTGGC TCTTGTGGAG
    AAATTCAATC TGGACATAAG CAAAGAGGAA TGTCAGCAGC TCATCGTGAA GTATGATTTA
    AAGAACAACG GGAAGTTTGC TTATTGCAAC TTCATCCAGA GCTGTGTCCT CCTGTTGAAA
    GCGAAGGAAA CCTCACTGAT GCAGAGGATG AAAATTCAGA ATGCACATAA AATGCTTTAC
    TGCGTATCCA GCCCAAAATT GTGCACTGCT GGAGGCCCAT GCGGCGTACC TTCAAAGCCT
    ATGATGAGGG CGGGACAGGG CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972769.1
    CDS316..4722
    Translation

    Target ORF information:

    RefSeq Version XM_014117294.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAACAGA ATAGCACCAA AACACACGTT TCCACAATCA ACAGCACCAA ATCCTGCAGT 
    ACCAAGGCCA GGGATTTAAT GTGCAAAATG GCAATTATAC CAGATTGGCA AACTTCGTAT
    CCTCACACAG GAAAGCTCAA GAATTTAAGA CCTCATTCTT CACCATGTAG AGTGTATTCA
    AGGAACAGTT TCCAAAATAT GTTCAGACCT TCTTCTTCAA CCACAGCTAT TGCAAATCCG
    ATACTGTCCT TCTTAGATGT TAAACGGCTT TTATTTCAAA AAATCACTGA TAAAGGATAT
    GCGTTGAAAA AGGCTTTTGA GCTGCTTGAC ACCAGTCACA ACATGACGGT ATCAAAGAAT
    GAACTGAGAA GAATTATCAC AACCTTCCTG ATGCCTCTCA CGAGAGAACA GTTTCAGGAT
    GTACTGGCTC AGATCCCCCT CACCAGCTCT GGGGCCATTC CATACCTTGA ATTCCTGTCC
    TGGTTTGGTG GAATGGACCT AAGTATAAAT GTTATACAGA GGGGAGGTGG GAATGAAATG
    AGTTGCAGTC GGACAGTAAA AGAACTTGAA AAACAAATAG GAGAAAAGAT TTTCAGAAAC
    ATTAAAACTA TTTTCAAAGC CTTCAAGCTC ATTGATGTTA GCAAGACTGG TCTGATCCAG
    TCTTGTGAAC TGAGGAAAGT TCTCGAGACC TTCTGTTTGA AGATGAAAGA TGATGAATAC
    AAAAAGTTTG CAAAACACTA CAACATTGAT AAAGATACTG CAGTGGATTA CAATGTTTTT
    TTGAAGAACC TCAGTGCAAA TAATGACTTG AACCTTAGAT ATTATATGGG AAACCAAGAT
    ATCTCCTGGA AGAATCAACC AGTCCAAAAT TCCAAAGGGG AGTGCCTCCA CAGTTCTGGT
    TCATCTGAAG ATGTCTGGAA AAACTACAAC CTAGACGAAA TTGAGAGAAC TTTTTGCCAA
    GAGCTTCCCA AGTGCTATGA AAAGGTTGAA AAAGCCCTGA GTGCAGGGGA CCCCTCCAAA
    AGTGGATATG TATCTTTGAA TTACCTAAAG ATTGTCCTCG ACACCTTTGT ATACCGATTA
    CCAAGGAGAA TTTTTATCCA GTTAATGAAA AGATTTGGAC TTAAAACCAC CACCAAAATC
    AATTGGAAAC AATTTCTAAC ATCACTTTAT GAACCCCAGT GGTTGGAAGT CAACAATACA
    GTTCCTCCAA CAAAAAGAAA TAGAATCACT TCTAGAGATC AATCTTGCAA GGAAAACATT
    ATCACAAAGT TATCTAGACA AGAGGATCAC TATACATCAT TGAAGAAAGC ACTCCTGATC
    GCCAACACTA AACCTGATGG ACAGATAACG GGGAAAGAAT TGCGATATAT TCTAAATTGC
    ATGGTTGTAA AAATCAGTGA TTCAGAATTC AAAGAGCTCA TGCAAATGCT TGACCCTGGG
    GACACCGGAT GGGTTAACAT CTACACACTT ATTGAACTGC TTGAAGAGAC CCCTAAGATA
    CAAAAACTCT CCTCCTCTGC AGACACCAAG GCGCCTCTCC TCCTGGCTTG GGATTCAGTG
    GAAGAAATCA TTCAGGACTC AATTGCTAGG AATCTCCAAG CCTTCTACAA AATGCTACGG
    TCATATGACC TCGGAGACAC AGGGCTCATT GGTCGAAATA ATTTCAAGAA AATCATGCGC
    ATCTTCTGTC CATTTTTAAC AATCGAACAT TTACTGAAAC TCTGTAGTAG GTTTCAGGAT
    ATTGCTTCAG GAAGAATCCT TTATAAGAAA TTTTTGGCAT CCATGGGGAT GAGTGGCCCA
    CCACCCCCCT CTCCGGTTAT TACTCCAAAG GATCAGCTAT TGAGTGACCA TTTTCAAAGA
    GAGGAGCAGC GGAAGTTGGA TCTTTCTGAA AGAACCAAGC CCGTGGAGGA CAGAAGCACC
    TCGACCAAGA AAATGACGAA GGATGAAGCG ATCGAAAAAC TAAAAAACTG TATTCAGCAG
    CGGGACCCAG CATTCAAAAA ATTCTTTCTT GACTATAGCA AGGAGCTCAC TGGCAAAATT
    CATGTGCGTG ACTTCAAGAA GGTACTGGAA GCTAGTGGAA TGCCCATGGA TGATGACCAG
    TACGCTCTGC TGACGGCAAA AATAGGATAT AAGAGAGAAG GGATGAGTTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATGC TAAAATGAGC AGGCCAGAAG TGATCCCATT GCAGACTCCA
    GTTCTTTCCA AGACTAATTT GGACAGATAC TTTATAACCG CTGAAGAGTG CCTGAAGCAG
    TTCCCCCAGA GACTGAAGGA AGCCTTCCGG GACCCCTACT CTGCCTTCTT TAAAATAGAC
    ACTGACAGGG ATGGCATAAT CAACATGCAT GATCTTCACA GATTGTTACT GCACTTACTG
    TTCAATCTTA AAGACAAAGA ATTTGAGCGT TTCCTGGGCC TTCTTGGCTT GAGACTCAGC
    GTCACTCTCA ATTTTCGGGA ATTTCGAAAT CTGTGTGAGA AGAGAGCATT GAGAACAGAT
    GACGCGCCTC AAAGACTAAT CAGACCAAAA CAGAAGGTCG CAGATTCAGA ACTAGCTTGT
    GAACAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAACCA GATGGTCAGA CTTATCTAAG
    AATTTTATAG AAACTGATAA TGAGGGCAAT GGCATCCTTC GGCGGCGGGA TATAAAGAAT
    GCGCTCTATG GATTTGATAT TCCTCTGACA CCGAGGGAAT TTGAAAAGCT CTGGATGAGC
    TATGACACCG AGGGAAGAGG GCACATAACT TACCAAGAAT TTCTACAGAA ATTGGGGATT
    AACTACTCAG CAGACATTCA TCGGCCCTAT GCAGAGGATT ACTTCAACTT CATGGGTCAT
    TTCACAAAAC CCCAGCAGAT GCAAGAGGAG GCCAGACAGC TAGAGCAGAG CGTGGACAGG
    ACTGCGCCAG TTAGGGATAA GCTTAAGGAC CATTATCAAG ATATCAGCAA AGCACTCACC
    AAACTAGACA AATCCAAAAA TGGCTACCTA TCCATGTGCA AGATGCAGAA AGTGCTGCAG
    GAGCTCGGCT GCTCTCTCAA GGAGGAGGAG CTGACCAATC TTCTGAACAG TTGGGAAATC
    AAATGGCATG ATAACTCCAT CAATTACCTT GACTTCCTGA AAGCAGTGGA GAGCAGCAAG
    CCAACAAGAC CTCAGCCAAA GAAAAAAGAA GAGAGTGTGC CAATCAGTTT CTCAACACTG
    AACCCAGAGG AGGTTGTAAA GAACATCCAG GAAGTTGTTG CCTCCTCCGA CATGGCTTTG
    TCAACGGCAT TTTCTGCATT GGATAAAGAG GACACTGGGT TTGTAAAGGC TTCAGATTTT
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACAGACA ACCAGTATCA TTATTTTTTG
    AGAAAACTGA AAATTCATCT AACCCCCCAT ATAAATTGGA AATATTTTCT CCAGAACTTC
    AGTTCTTTCT CTGAAGAGAC TGCTGCCGAG TGGGCTGAGA AAATGCCCAA AGGCCCACCG
    CCCACATCTC CTCCCAAGGA AACAGCAAAC CAGGAGCTGC TGGCTCAGCT CCACAAGGCG
    GTGGGCTCCC ACTACCATGC GATTGCTCAG GAGTTTGACA ACTTCGACAC CATGAAAACG
    TACACCGCCT CCAAGGATGA ATTCAGGGCC ATTTGTGCTC GCCATGTCCA AATTCTGACA
    GATGAGCAAT TTGACAGACT GTGGAGCAAG ATGCCAGTCA ATGCCAAGGG GAGGCTGAAG
    TACCTGGACC TCCTGAGCAT GTTCAGCTCC AAGAGAGCGC CAACACCATT GACCACGGGC
    AGCTCGGCCA AGGCCCAGAG AGGCAGCAGT GTTCCTGAAA TCTCAGATGG AAGCAGGCCT
    GCAGTATCAT CGCCCATTCA TGACCTGAAG GCGGGGTTGA AAGCACAGAG CCACCCCTGT
    ACCCCCGCTA GTGCCAGTTC GGCGCCAGGC ACCCTGCCCT TGCAGAACTG CGAGCCCATA
    GAAAGCAAGC TCCAGAAGAA AATCCAGGGC TGCTGGCGAG AGTTCCTGAA AGAATGCAAA
    GAGAAGGATG TGAATAAGCA GGGCCAGGTC ACCACGGCTG AATTCCTGGC TCTTGTGGAG
    AAATTCAATC TGGACATAAG CAAAGAGGAA TGTCAGCAGC TCATCGTGAA GTATGATTTA
    AAGAACAACG GGAAGTTTGC TTATTGCAAC TTCATCCAGA GCTGTGTCCT CCTGTTGAAA
    GCGAAGGAAA CCTCACTGAT GCAGAGGATG AAAATTCAGA ATGCACATAA AATGCTTTAC
    TGCGTATCCA GCCCAAAATT GTGCACTGCT GGAGGCCCAT GCGGCGTACC TTCAAAGCCT
    ATGATGAGGG CGGGACAGGG CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38866
    Clone ID Related Accession (Same CDS sequence) XM_014117294.1 , XM_014117294.2
    Accession Version XM_014117294.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAACAGA ATAGCACCAA AACACACGTT TCCACAATCA ACAGCACCAA ATCCTGCAGT 
    ACCAAGGCCA GGGATTTAAT GTGCAAAATG GCAATTATAC CAGATTGGCA AACTTCGTAT
    CCTCACACAG GAAAGCTCAA GAATTTAAGA CCTCATTCTT CACCATGTAG AGTGTATTCA
    AGGAACAGTT TCCAAAATAT GTTCAGACCT TCTTCTTCAA CCACAGCTAT TGCAAATCCG
    ATACTGTCCT TCTTAGATGT TAAACGGCTT TTATTTCAAA AAATCACTGA TAAAGGATAT
    GCGTTGAAAA AGGCTTTTGA GCTGCTTGAC ACCAGTCACA ACATGACGGT ATCAAAGAAT
    GAACTGAGAA GAATTATCAC AACCTTCCTG ATGCCTCTCA CGAGAGAACA GTTTCAGGAT
    GTACTGGCTC AGATCCCCCT CACCAGCTCT GGGGCCATTC CATACCTTGA ATTCCTGTCC
    TGGTTTGGTG GAATGGACCT AAGTATAAAT GTTATACAGA GGGGAGGTGG GAATGAAATG
    AGTTGCAGTC GGACAGTAAA AGAACTTGAA AAACAAATAG GAGAAAAGAT TTTCAGAAAC
    ATTAAAACTA TTTTCAAAGC CTTCAAGCTC ATTGATGTTA GCAAGACTGG TCTGATCCAG
    TCTTGTGAAC TGAGGAAAGT TCTCGAGACC TTCTGTTTGA AGATGAAAGA TGATGAATAC
    AAAAAGTTTG CAAAACACTA CAACATTGAT AAAGATACTG CAGTGGATTA CAATGTTTTT
    TTGAAGAACC TCAGTGCAAA TAATGACTTG AACCTTAGAT ATTATATGGG AAACCAAGAT
    ATCTCCTGGA AGAATCAACC AGTCCAAAAT TCCAAAGGGG AGTGCCTCCA CAGTTCTGGT
    TCATCTGAAG ATGTCTGGAA AAACTACAAC CTAGACGAAA TTGAGAGAAC TTTTTGCCAA
    GAGCTTCCCA AGTGCTATGA AAAGGTTGAA AAAGCCCTGA GTGCAGGGGA CCCCTCCAAA
    AGTGGATATG TATCTTTGAA TTACCTAAAG ATTGTCCTCG ACACCTTTGT ATACCGATTA
    CCAAGGAGAA TTTTTATCCA GTTAATGAAA AGATTTGGAC TTAAAACCAC CACCAAAATC
    AATTGGAAAC AATTTCTAAC ATCACTTTAT GAACCCCAGT GGTTGGAAGT CAACAATACA
    GTTCCTCCAA CAAAAAGAAA TAGAATCACT TCTAGAGATC AATCTTGCAA GGAAAACATT
    ATCACAAAGT TATCTAGACA AGAGGATCAC TATACATCAT TGAAGAAAGC ACTCCTGATC
    GCCAACACTA AACCTGATGG ACAGATAACG GGGAAAGAAT TGCGATATAT TCTAAATTGC
    ATGGTTGTAA AAATCAGTGA TTCAGAATTC AAAGAGCTCA TGCAAATGCT TGACCCTGGG
    GACACCGGAT GGGTTAACAT CTACACACTT ATTGAACTGC TTGAAGAGAC CCCTAAGATA
    CAAAAACTCT CCTCCTCTGC AGACACCAAG GCGCCTCTCC TCCTGGCTTG GGATTCAGTG
    GAAGAAATCA TTCAGGACTC AATTGCTAGG AATCTCCAAG CCTTCTACAA AATGCTACGG
    TCATATGACC TCGGAGACAC AGGGCTCATT GGTCGAAATA ATTTCAAGAA AATCATGCGC
    ATCTTCTGTC CATTTTTAAC AATCGAACAT TTACTGAAAC TCTGTAGTAG GTTTCAGGAT
    ATTGCTTCAG GAAGAATCCT TTATAAGAAA TTTTTGGCAT CCATGGGGAT GAGTGGCCCA
    CCACCCCCCT CTCCGGTTAT TACTCCAAAG GATCAGCTAT TGAGTGACCA TTTTCAAAGA
    GAGGAGCAGC GGAAGTTGGA TCTTTCTGAA AGAACCAAGC CCGTGGAGGA CAGAAGCACC
    TCGACCAAGA AAATGACGAA GGATGAAGCG ATCGAAAAAC TAAAAAACTG TATTCAGCAG
    CGGGACCCAG CATTCAAAAA ATTCTTTCTT GACTATAGCA AGGAGCTCAC TGGCAAAATT
    CATGTGCGTG ACTTCAAGAA GGTACTGGAA GCTAGTGGAA TGCCCATGGA TGATGACCAG
    TACGCTCTGC TGACGGCAAA AATAGGATAT AAGAGAGAAG GGATGAGTTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATGC TAAAATGAGC AGGCCAGAAG TGATCCCATT GCAGACTCCA
    GTTCTTTCCA AGACTAATTT GGACAGATAC TTTATAACCG CTGAAGAGTG CCTGAAGCAG
    TTCCCCCAGA GACTGAAGGA AGCCTTCCGG GACCCCTACT CTGCCTTCTT TAAAATAGAC
    ACTGACAGGG ATGGCATAAT CAACATGCAT GATCTTCACA GATTGTTACT GCACTTACTG
    TTCAATCTTA AAGACAAAGA ATTTGAGCGT TTCCTGGGCC TTCTTGGCTT GAGACTCAGC
    GTCACTCTCA ATTTTCGGGA ATTTCGAAAT CTGTGTGAGA AGAGAGCATT GAGAACAGAT
    GACGCGCCTC AAAGACTAAT CAGACCAAAA CAGAAGGTCG CAGATTCAGA ACTAGCTTGT
    GAACAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAACCA GATGGTCAGA CTTATCTAAG
    AATTTTATAG AAACTGATAA TGAGGGCAAT GGCATCCTTC GGCGGCGGGA TATAAAGAAT
    GCGCTCTATG GATTTGATAT TCCTCTGACA CCGAGGGAAT TTGAAAAGCT CTGGATGAGC
    TATGACACCG AGGGAAGAGG GCACATAACT TACCAAGAAT TTCTACAGAA ATTGGGGATT
    AACTACTCAG CAGACATTCA TCGGCCCTAT GCAGAGGATT ACTTCAACTT CATGGGTCAT
    TTCACAAAAC CCCAGCAGAT GCAAGAGGAG GCCAGACAGC TAGAGCAGAG CGTGGACAGG
    ACTGCGCCAG TTAGGGATAA GCTTAAGGAC CATTATCAAG ATATCAGCAA AGCACTCACC
    AAACTAGACA AATCCAAAAA TGGCTACCTA TCCATGTGCA AGATGCAGAA AGTGCTGCAG
    GAGCTCGGCT GCTCTCTCAA GGAGGAGGAG CTGACCAATC TTCTGAACAG TTGGGAAATC
    AAATGGCATG ATAACTCCAT CAATTACCTT GACTTCCTGA AAGCAGTGGA GAGCAGCAAG
    CCAACAAGAC CTCAGCCAAA GAAAAAAGAA GAGAGTGTGC CAATCAGTTT CTCAACACTG
    AACCCAGAGG AGGTTGTAAA GAACATCCAG GAAGTTGTTG CCTCCTCCGA CATGGCTTTG
    TCAACGGCAT TTTCTGCATT GGATAAAGAG GACACTGGGT TTGTAAAGGC TTCAGATTTT
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACAGACA ACCAGTATCA TTATTTTTTG
    AGAAAACTGA AAATTCATCT AACCCCCCAT ATAAATTGGA AATATTTTCT CCAGAACTTC
    AGTTCTTTCT CTGAAGAGAC TGCTGCCGAG TGGGCTGAGA AAATGCCCAA AGGCCCACCG
    CCCACATCTC CTCCCAAGGA AACAGCAAAC CAGGAGCTGC TGGCTCAGCT CCACAAGGCG
    GTGGGCTCCC ACTACCATGC GATTGCTCAG GAGTTTGACA ACTTCGACAC CATGAAAACG
    TACACCGCCT CCAAGGATGA ATTCAGGGCC ATTTGTGCTC GCCATGTCCA AATTCTGACA
    GATGAGCAAT TTGACAGACT GTGGAGCAAG ATGCCAGTCA ATGCCAAGGG GAGGCTGAAG
    TACCTGGACC TCCTGAGCAT GTTCAGCTCC AAGAGAGCGC CAACACCATT GACCACGGGC
    AGCTCGGCCA AGGCCCAGAG AGGCAGCAGT GTTCCTGAAA TCTCAGATGG AAGCAGGCCT
    GCAGTATCAT CGCCCATTCA TGACCTGAAG GCGGGGTTGA AAGCACAGAG CCACCCCTGT
    ACCCCCGCTA GTGCCAGTTC GGCGCCAGGC ACCCTGCCCT TGCAGAACTG CGAGCCCATA
    GAAAGCAAGC TCCAGAAGAA AATCCAGGGC TGCTGGCGAG AGTTCCTGAA AGAATGCAAA
    GAGAAGGATG TGAATAAGCA GGGCCAGGTC ACCACGGCTG AATTCCTGGC TCTTGTGGAG
    AAATTCAATC TGGACATAAG CAAAGAGGAA TGTCAGCAGC TCATCGTGAA GTATGATTTA
    AAGAACAACG GGAAGTTTGC TTATTGCAAC TTCATCCAGA GCTGTGTCCT CCTGTTGAAA
    GCGAAGGAAA CCTCACTGAT GCAGAGGATG AAAATTCAGA ATGCACATAA AATGCTTTAC
    TGCGTATCCA GCCCAAAATT GTGCACTGCT GGAGGCCCAT GCGGCGTACC TTCAAAGCCT
    ATGATGAGGG CGGGACAGGG CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972769.1
    CDS333..4739
    Translation

    Target ORF information:

    RefSeq Version XM_014117294.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117294.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGAAACAGA ATAGCACCAA AACACACGTT TCCACAATCA ACAGCACCAA ATCCTGCAGT 
    ACCAAGGCCA GGGATTTAAT GTGCAAAATG GCAATTATAC CAGATTGGCA AACTTCGTAT
    CCTCACACAG GAAAGCTCAA GAATTTAAGA CCTCATTCTT CACCATGTAG AGTGTATTCA
    AGGAACAGTT TCCAAAATAT GTTCAGACCT TCTTCTTCAA CCACAGCTAT TGCAAATCCG
    ATACTGTCCT TCTTAGATGT TAAACGGCTT TTATTTCAAA AAATCACTGA TAAAGGATAT
    GCGTTGAAAA AGGCTTTTGA GCTGCTTGAC ACCAGTCACA ACATGACGGT ATCAAAGAAT
    GAACTGAGAA GAATTATCAC AACCTTCCTG ATGCCTCTCA CGAGAGAACA GTTTCAGGAT
    GTACTGGCTC AGATCCCCCT CACCAGCTCT GGGGCCATTC CATACCTTGA ATTCCTGTCC
    TGGTTTGGTG GAATGGACCT AAGTATAAAT GTTATACAGA GGGGAGGTGG GAATGAAATG
    AGTTGCAGTC GGACAGTAAA AGAACTTGAA AAACAAATAG GAGAAAAGAT TTTCAGAAAC
    ATTAAAACTA TTTTCAAAGC CTTCAAGCTC ATTGATGTTA GCAAGACTGG TCTGATCCAG
    TCTTGTGAAC TGAGGAAAGT TCTCGAGACC TTCTGTTTGA AGATGAAAGA TGATGAATAC
    AAAAAGTTTG CAAAACACTA CAACATTGAT AAAGATACTG CAGTGGATTA CAATGTTTTT
    TTGAAGAACC TCAGTGCAAA TAATGACTTG AACCTTAGAT ATTATATGGG AAACCAAGAT
    ATCTCCTGGA AGAATCAACC AGTCCAAAAT TCCAAAGGGG AGTGCCTCCA CAGTTCTGGT
    TCATCTGAAG ATGTCTGGAA AAACTACAAC CTAGACGAAA TTGAGAGAAC TTTTTGCCAA
    GAGCTTCCCA AGTGCTATGA AAAGGTTGAA AAAGCCCTGA GTGCAGGGGA CCCCTCCAAA
    AGTGGATATG TATCTTTGAA TTACCTAAAG ATTGTCCTCG ACACCTTTGT ATACCGATTA
    CCAAGGAGAA TTTTTATCCA GTTAATGAAA AGATTTGGAC TTAAAACCAC CACCAAAATC
    AATTGGAAAC AATTTCTAAC ATCACTTTAT GAACCCCAGT GGTTGGAAGT CAACAATACA
    GTTCCTCCAA CAAAAAGAAA TAGAATCACT TCTAGAGATC AATCTTGCAA GGAAAACATT
    ATCACAAAGT TATCTAGACA AGAGGATCAC TATACATCAT TGAAGAAAGC ACTCCTGATC
    GCCAACACTA AACCTGATGG ACAGATAACG GGGAAAGAAT TGCGATATAT TCTAAATTGC
    ATGGTTGTAA AAATCAGTGA TTCAGAATTC AAAGAGCTCA TGCAAATGCT TGACCCTGGG
    GACACCGGAT GGGTTAACAT CTACACACTT ATTGAACTGC TTGAAGAGAC CCCTAAGATA
    CAAAAACTCT CCTCCTCTGC AGACACCAAG GCGCCTCTCC TCCTGGCTTG GGATTCAGTG
    GAAGAAATCA TTCAGGACTC AATTGCTAGG AATCTCCAAG CCTTCTACAA AATGCTACGG
    TCATATGACC TCGGAGACAC AGGGCTCATT GGTCGAAATA ATTTCAAGAA AATCATGCGC
    ATCTTCTGTC CATTTTTAAC AATCGAACAT TTACTGAAAC TCTGTAGTAG GTTTCAGGAT
    ATTGCTTCAG GAAGAATCCT TTATAAGAAA TTTTTGGCAT CCATGGGGAT GAGTGGCCCA
    CCACCCCCCT CTCCGGTTAT TACTCCAAAG GATCAGCTAT TGAGTGACCA TTTTCAAAGA
    GAGGAGCAGC GGAAGTTGGA TCTTTCTGAA AGAACCAAGC CCGTGGAGGA CAGAAGCACC
    TCGACCAAGA AAATGACGAA GGATGAAGCG ATCGAAAAAC TAAAAAACTG TATTCAGCAG
    CGGGACCCAG CATTCAAAAA ATTCTTTCTT GACTATAGCA AGGAGCTCAC TGGCAAAATT
    CATGTGCGTG ACTTCAAGAA GGTACTGGAA GCTAGTGGAA TGCCCATGGA TGATGACCAG
    TACGCTCTGC TGACGGCAAA AATAGGATAT AAGAGAGAAG GGATGAGTTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATGC TAAAATGAGC AGGCCAGAAG TGATCCCATT GCAGACTCCA
    GTTCTTTCCA AGACTAATTT GGACAGATAC TTTATAACCG CTGAAGAGTG CCTGAAGCAG
    TTCCCCCAGA GACTGAAGGA AGCCTTCCGG GACCCCTACT CTGCCTTCTT TAAAATAGAC
    ACTGACAGGG ATGGCATAAT CAACATGCAT GATCTTCACA GATTGTTACT GCACTTACTG
    TTCAATCTTA AAGACAAAGA ATTTGAGCGT TTCCTGGGCC TTCTTGGCTT GAGACTCAGC
    GTCACTCTCA ATTTTCGGGA ATTTCGAAAT CTGTGTGAGA AGAGAGCATT GAGAACAGAT
    GACGCGCCTC AAAGACTAAT CAGACCAAAA CAGAAGGTCG CAGATTCAGA ACTAGCTTGT
    GAACAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAACCA GATGGTCAGA CTTATCTAAG
    AATTTTATAG AAACTGATAA TGAGGGCAAT GGCATCCTTC GGCGGCGGGA TATAAAGAAT
    GCGCTCTATG GATTTGATAT TCCTCTGACA CCGAGGGAAT TTGAAAAGCT CTGGATGAGC
    TATGACACCG AGGGAAGAGG GCACATAACT TACCAAGAAT TTCTACAGAA ATTGGGGATT
    AACTACTCAG CAGACATTCA TCGGCCCTAT GCAGAGGATT ACTTCAACTT CATGGGTCAT
    TTCACAAAAC CCCAGCAGAT GCAAGAGGAG GCCAGACAGC TAGAGCAGAG CGTGGACAGG
    ACTGCGCCAG TTAGGGATAA GCTTAAGGAC CATTATCAAG ATATCAGCAA AGCACTCACC
    AAACTAGACA AATCCAAAAA TGGCTACCTA TCCATGTGCA AGATGCAGAA AGTGCTGCAG
    GAGCTCGGCT GCTCTCTCAA GGAGGAGGAG CTGACCAATC TTCTGAACAG TTGGGAAATC
    AAATGGCATG ATAACTCCAT CAATTACCTT GACTTCCTGA AAGCAGTGGA GAGCAGCAAG
    CCAACAAGAC CTCAGCCAAA GAAAAAAGAA GAGAGTGTGC CAATCAGTTT CTCAACACTG
    AACCCAGAGG AGGTTGTAAA GAACATCCAG GAAGTTGTTG CCTCCTCCGA CATGGCTTTG
    TCAACGGCAT TTTCTGCATT GGATAAAGAG GACACTGGGT TTGTAAAGGC TTCAGATTTT
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACAGACA ACCAGTATCA TTATTTTTTG
    AGAAAACTGA AAATTCATCT AACCCCCCAT ATAAATTGGA AATATTTTCT CCAGAACTTC
    AGTTCTTTCT CTGAAGAGAC TGCTGCCGAG TGGGCTGAGA AAATGCCCAA AGGCCCACCG
    CCCACATCTC CTCCCAAGGA AACAGCAAAC CAGGAGCTGC TGGCTCAGCT CCACAAGGCG
    GTGGGCTCCC ACTACCATGC GATTGCTCAG GAGTTTGACA ACTTCGACAC CATGAAAACG
    TACACCGCCT CCAAGGATGA ATTCAGGGCC ATTTGTGCTC GCCATGTCCA AATTCTGACA
    GATGAGCAAT TTGACAGACT GTGGAGCAAG ATGCCAGTCA ATGCCAAGGG GAGGCTGAAG
    TACCTGGACC TCCTGAGCAT GTTCAGCTCC AAGAGAGCGC CAACACCATT GACCACGGGC
    AGCTCGGCCA AGGCCCAGAG AGGCAGCAGT GTTCCTGAAA TCTCAGATGG AAGCAGGCCT
    GCAGTATCAT CGCCCATTCA TGACCTGAAG GCGGGGTTGA AAGCACAGAG CCACCCCTGT
    ACCCCCGCTA GTGCCAGTTC GGCGCCAGGC ACCCTGCCCT TGCAGAACTG CGAGCCCATA
    GAAAGCAAGC TCCAGAAGAA AATCCAGGGC TGCTGGCGAG AGTTCCTGAA AGAATGCAAA
    GAGAAGGATG TGAATAAGCA GGGCCAGGTC ACCACGGCTG AATTCCTGGC TCTTGTGGAG
    AAATTCAATC TGGACATAAG CAAAGAGGAA TGTCAGCAGC TCATCGTGAA GTATGATTTA
    AAGAACAACG GGAAGTTTGC TTATTGCAAC TTCATCCAGA GCTGTGTCCT CCTGTTGAAA
    GCGAAGGAAA CCTCACTGAT GCAGAGGATG AAAATTCAGA ATGCACATAA AATGCTTTAC
    TGCGTATCCA GCCCAAAATT GTGCACTGCT GGAGGCCCAT GCGGCGTACC TTCAAAGCCT
    ATGATGAGGG CGGGACAGGG CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38867
    Clone ID Related Accession (Same CDS sequence) XM_014117295.1 , XM_014117295.2
    Accession Version XM_014117295.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACCTAA GTATAAATGT TATACAGAGG GGAGGTGGGA ATGAAATGAG TTGCAGTCGG 
    ACAGTAAAAG AACTTGAAAA ACAAATAGGA GAAAAGATTT TCAGAAACAT TAAAACTATT
    TTCAAAGCCT TCAAGCTCAT TGATGTTAGC AAGACTGGTC TGATCCAGTC TTGTGAACTG
    AGGAAAGTTC TCGAGACCTT CTGTTTGAAG ATGAAAGATG ATGAATACAA AAAGTTTGCA
    AAACACTACA ACATTGATAA AGATACTGCA GTGGATTACA ATGTTTTTTT GAAGAACCTC
    AGTGCAAATA ATGACTTGAA CCTTAGATAT TATATGGGAA ACCAAGATAT CTCCTGGAAG
    AATCAACCAG TCCAAAATTC CAAAGGGGAG TGCCTCCACA GTTCTGGTTC ATCTGAAGAT
    GTCTGGAAAA ACTACAACCT AGACGAAATT GAGAGAACTT TTTGCCAAGA GCTTCCCAAG
    TGCTATGAAA AGGTTGAAAA AGCCCTGAGT GCAGGGGACC CCTCCAAAAG TGGATATGTA
    TCTTTGAATT ACCTAAAGAT TGTCCTCGAC ACCTTTGTAT ACCGATTACC AAGGAGAATT
    TTTATCCAGT TAATGAAAAG ATTTGGACTT AAAACCACCA CCAAAATCAA TTGGAAACAA
    TTTCTAACAT CACTTTATGA ACCCCAGTGG TTGGAAGTCA ACAATACAGT TCCTCCAACA
    AAAAGAAATA GAATCACTTC TAGAGATCAA TCTTGCAAGG AAAACATTAT CACAAAGTTA
    TCTAGACAAG AGGATCACTA TACATCATTG AAGAAAGCAC TCCTGATCGC CAACACTAAA
    CCTGATGGAC AGATAACGGG GAAAGAATTG CGATATATTC TAAATTGCAT GGTTGTAAAA
    ATCAGTGATT CAGAATTCAA AGAGCTCATG CAAATGCTTG ACCCTGGGGA CACCGGATGG
    GTTAACATCT ACACACTTAT TGAACTGCTT GAAGAGACCC CTAAGATACA AAAACTCTCC
    TCCTCTGCAG ACACCAAGGC GCCTCTCCTC CTGGCTTGGG ATTCAGTGGA AGAAATCATT
    CAGGACTCAA TTGCTAGGAA TCTCCAAGCC TTCTACAAAA TGCTACGGTC ATATGACCTC
    GGAGACACAG GGCTCATTGG TCGAAATAAT TTCAAGAAAA TCATGCGCAT CTTCTGTCCA
    TTTTTAACAA TCGAACATTT ACTGAAACTC TGTAGTAGGT TTCAGGATAT TGCTTCAGGA
    AGAATCCTTT ATAAGAAATT TTTGGCATCC ATGGGGATGA GTGGCCCACC ACCCCCCTCT
    CCGGTTATTA CTCCAAAGGA TCAGCTATTG AGTGACCATT TTCAAAGAGA GGAGCAGCGG
    AAGTTGGATC TTTCTGAAAG AACCAAGCCC GTGGAGGACA GAAGCACCTC GACCAAGAAA
    ATGACGAAGG ATGAAGCGAT CGAAAAACTA AAAAACTGTA TTCAGCAGCG GGACCCAGCA
    TTCAAAAAAT TCTTTCTTGA CTATAGCAAG GAGCTCACTG GCAAAATTCA TGTGCGTGAC
    TTCAAGAAGG TACTGGAAGC TAGTGGAATG CCCATGGATG ATGACCAGTA CGCTCTGCTG
    ACGGCAAAAA TAGGATATAA GAGAGAAGGG ATGAGTTATC TTGATTTTGC AGCAGGATTT
    GAAGATGCTA AAATGAGCAG GCCAGAAGTG ATCCCATTGC AGACTCCAGT TCTTTCCAAG
    ACTAATTTGG ACAGATACTT TATAACCGCT GAAGAGTGCC TGAAGCAGTT CCCCCAGAGA
    CTGAAGGAAG CCTTCCGGGA CCCCTACTCT GCCTTCTTTA AAATAGACAC TGACAGGGAT
    GGCATAATCA ACATGCATGA TCTTCACAGA TTGTTACTGC ACTTACTGTT CAATCTTAAA
    GACAAAGAAT TTGAGCGTTT CCTGGGCCTT CTTGGCTTGA GACTCAGCGT CACTCTCAAT
    TTTCGGGAAT TTCGAAATCT GTGTGAGAAG AGAGCATTGA GAACAGATGA CGCGCCTCAA
    AGACTAATCA GACCAAAACA GAAGGTCGCA GATTCAGAAC TAGCTTGTGA ACAGGCTCAT
    CAGTATCTTG TTACCAAAGC AAAAACCAGA TGGTCAGACT TATCTAAGAA TTTTATAGAA
    ACTGATAATG AGGGCAATGG CATCCTTCGG CGGCGGGATA TAAAGAATGC GCTCTATGGA
    TTTGATATTC CTCTGACACC GAGGGAATTT GAAAAGCTCT GGATGAGCTA TGACACCGAG
    GGAAGAGGGC ACATAACTTA CCAAGAATTT CTACAGAAAT TGGGGATTAA CTACTCAGCA
    GACATTCATC GGCCCTATGC AGAGGATTAC TTCAACTTCA TGGGTCATTT CACAAAACCC
    CAGCAGATGC AAGAGGAGGC CAGACAGCTA GAGCAGAGCG TGGACAGGAC TGCGCCAGTT
    AGGGATAAGC TTAAGGACCA TTATCAAGAT ATCAGCAAAG CACTCACCAA ACTAGACAAA
    TCCAAAAATG GCTACCTATC CATGTGCAAG ATGCAGAAAG TGCTGCAGGA GCTCGGCTGC
    TCTCTCAAGG AGGAGGAGCT GACCAATCTT CTGAACAGTT GGGAAATCAA ATGGCATGAT
    AACTCCATCA ATTACCTTGA CTTCCTGAAA GCAGTGGAGA GCAGCAAGCC AACAAGACCT
    CAGCCAAAGA AAAAAGAAGA GAGTGTGCCA ATCAGTTTCT CAACACTGAA CCCAGAGGAG
    GTTGTAAAGA ACATCCAGGA AGTTGTTGCC TCCTCCGACA TGGCTTTGTC AACGGCATTT
    TCTGCATTGG ATAAAGAGGA CACTGGGTTT GTAAAGGCTT CAGATTTTGG ACAAGTTCTT
    AAGGATTTCT GTTACAAACT AACAGACAAC CAGTATCATT ATTTTTTGAG AAAACTGAAA
    ATTCATCTAA CCCCCCATAT AAATTGGAAA TATTTTCTCC AGAACTTCAG TTCTTTCTCT
    GAAGAGACTG CTGCCGAGTG GGCTGAGAAA ATGCCCAAAG GCCCACCGCC CACATCTCCT
    CCCAAGGAAA CAGCAAACCA GGAGCTGCTG GCTCAGCTCC ACAAGGCGGT GGGCTCCCAC
    TACCATGCGA TTGCTCAGGA GTTTGACAAC TTCGACACCA TGAAAACGTA CACCGCCTCC
    AAGGATGAAT TCAGGGCCAT TTGTGCTCGC CATGTCCAAA TTCTGACAGA TGAGCAATTT
    GACAGACTGT GGAGCAAGAT GCCAGTCAAT GCCAAGGGGA GGCTGAAGTA CCTGGACCTC
    CTGAGCATGT TCAGCTCCAA GAGAGCGCCA ACACCATTGA CCACGGGCAG CTCGGCCAAG
    GCCCAGAGAG GCAGCAGTGT TCCTGAAATC TCAGATGGAA GCAGGCCTGC AGTATCATCG
    CCCATTCATG ACCTGAAGGC GGGGTTGAAA GCACAGAGCC ACCCCTGTAC CCCCGCTAGT
    GCCAGTTCGG CGCCAGGCAC CCTGCCCTTG CAGAACTGCG AGCCCATAGA AAGCAAGCTC
    CAGAAGAAAA TCCAGGGCTG CTGGCGAGAG TTCCTGAAAG AATGCAAAGA GAAGGATGTG
    AATAAGCAGG GCCAGGTCAC CACGGCTGAA TTCCTGGCTC TTGTGGAGAA ATTCAATCTG
    GACATAAGCA AAGAGGAATG TCAGCAGCTC ATCGTGAAGT ATGATTTAAA GAACAACGGG
    AAGTTTGCTT ATTGCAACTT CATCCAGAGC TGTGTCCTCC TGTTGAAAGC GAAGGAAACC
    TCACTGATGC AGAGGATGAA AATTCAGAAT GCACATAAAA TGAAAGAATC TGGTGCTGAG
    ACTTCTTCCT TTTATTCAGC TTTACTGCGT ATCCAGCCCA AAATTGTGCA CTGCTGGAGG
    CCCATGCGGC GTACCTTCAA AGCCTATGAT GAGGGCGGGA CAGGGCTTTT AAGCGTGGCT
    GATTTCAGGA AGGTCCTGAG ACAGTACAGC ATCAACCTCT CGGAGGAGGA GTTCTTTCAC
    ATTCTGGAGT ATTATGACAA GACGCTGTCT TCCAAGATCT CCTACAACGA CTTCCTGCGG
    GCGTTCCTTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972770.1
    CDS141..4235
    Translation

    Target ORF information:

    RefSeq Version XM_014117295.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117295.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACCTAA GTATAAATGT TATACAGAGG GGAGGTGGGA ATGAAATGAG TTGCAGTCGG 
    ACAGTAAAAG AACTTGAAAA ACAAATAGGA GAAAAGATTT TCAGAAACAT TAAAACTATT
    TTCAAAGCCT TCAAGCTCAT TGATGTTAGC AAGACTGGTC TGATCCAGTC TTGTGAACTG
    AGGAAAGTTC TCGAGACCTT CTGTTTGAAG ATGAAAGATG ATGAATACAA AAAGTTTGCA
    AAACACTACA ACATTGATAA AGATACTGCA GTGGATTACA ATGTTTTTTT GAAGAACCTC
    AGTGCAAATA ATGACTTGAA CCTTAGATAT TATATGGGAA ACCAAGATAT CTCCTGGAAG
    AATCAACCAG TCCAAAATTC CAAAGGGGAG TGCCTCCACA GTTCTGGTTC ATCTGAAGAT
    GTCTGGAAAA ACTACAACCT AGACGAAATT GAGAGAACTT TTTGCCAAGA GCTTCCCAAG
    TGCTATGAAA AGGTTGAAAA AGCCCTGAGT GCAGGGGACC CCTCCAAAAG TGGATATGTA
    TCTTTGAATT ACCTAAAGAT TGTCCTCGAC ACCTTTGTAT ACCGATTACC AAGGAGAATT
    TTTATCCAGT TAATGAAAAG ATTTGGACTT AAAACCACCA CCAAAATCAA TTGGAAACAA
    TTTCTAACAT CACTTTATGA ACCCCAGTGG TTGGAAGTCA ACAATACAGT TCCTCCAACA
    AAAAGAAATA GAATCACTTC TAGAGATCAA TCTTGCAAGG AAAACATTAT CACAAAGTTA
    TCTAGACAAG AGGATCACTA TACATCATTG AAGAAAGCAC TCCTGATCGC CAACACTAAA
    CCTGATGGAC AGATAACGGG GAAAGAATTG CGATATATTC TAAATTGCAT GGTTGTAAAA
    ATCAGTGATT CAGAATTCAA AGAGCTCATG CAAATGCTTG ACCCTGGGGA CACCGGATGG
    GTTAACATCT ACACACTTAT TGAACTGCTT GAAGAGACCC CTAAGATACA AAAACTCTCC
    TCCTCTGCAG ACACCAAGGC GCCTCTCCTC CTGGCTTGGG ATTCAGTGGA AGAAATCATT
    CAGGACTCAA TTGCTAGGAA TCTCCAAGCC TTCTACAAAA TGCTACGGTC ATATGACCTC
    GGAGACACAG GGCTCATTGG TCGAAATAAT TTCAAGAAAA TCATGCGCAT CTTCTGTCCA
    TTTTTAACAA TCGAACATTT ACTGAAACTC TGTAGTAGGT TTCAGGATAT TGCTTCAGGA
    AGAATCCTTT ATAAGAAATT TTTGGCATCC ATGGGGATGA GTGGCCCACC ACCCCCCTCT
    CCGGTTATTA CTCCAAAGGA TCAGCTATTG AGTGACCATT TTCAAAGAGA GGAGCAGCGG
    AAGTTGGATC TTTCTGAAAG AACCAAGCCC GTGGAGGACA GAAGCACCTC GACCAAGAAA
    ATGACGAAGG ATGAAGCGAT CGAAAAACTA AAAAACTGTA TTCAGCAGCG GGACCCAGCA
    TTCAAAAAAT TCTTTCTTGA CTATAGCAAG GAGCTCACTG GCAAAATTCA TGTGCGTGAC
    TTCAAGAAGG TACTGGAAGC TAGTGGAATG CCCATGGATG ATGACCAGTA CGCTCTGCTG
    ACGGCAAAAA TAGGATATAA GAGAGAAGGG ATGAGTTATC TTGATTTTGC AGCAGGATTT
    GAAGATGCTA AAATGAGCAG GCCAGAAGTG ATCCCATTGC AGACTCCAGT TCTTTCCAAG
    ACTAATTTGG ACAGATACTT TATAACCGCT GAAGAGTGCC TGAAGCAGTT CCCCCAGAGA
    CTGAAGGAAG CCTTCCGGGA CCCCTACTCT GCCTTCTTTA AAATAGACAC TGACAGGGAT
    GGCATAATCA ACATGCATGA TCTTCACAGA TTGTTACTGC ACTTACTGTT CAATCTTAAA
    GACAAAGAAT TTGAGCGTTT CCTGGGCCTT CTTGGCTTGA GACTCAGCGT CACTCTCAAT
    TTTCGGGAAT TTCGAAATCT GTGTGAGAAG AGAGCATTGA GAACAGATGA CGCGCCTCAA
    AGACTAATCA GACCAAAACA GAAGGTCGCA GATTCAGAAC TAGCTTGTGA ACAGGCTCAT
    CAGTATCTTG TTACCAAAGC AAAAACCAGA TGGTCAGACT TATCTAAGAA TTTTATAGAA
    ACTGATAATG AGGGCAATGG CATCCTTCGG CGGCGGGATA TAAAGAATGC GCTCTATGGA
    TTTGATATTC CTCTGACACC GAGGGAATTT GAAAAGCTCT GGATGAGCTA TGACACCGAG
    GGAAGAGGGC ACATAACTTA CCAAGAATTT CTACAGAAAT TGGGGATTAA CTACTCAGCA
    GACATTCATC GGCCCTATGC AGAGGATTAC TTCAACTTCA TGGGTCATTT CACAAAACCC
    CAGCAGATGC AAGAGGAGGC CAGACAGCTA GAGCAGAGCG TGGACAGGAC TGCGCCAGTT
    AGGGATAAGC TTAAGGACCA TTATCAAGAT ATCAGCAAAG CACTCACCAA ACTAGACAAA
    TCCAAAAATG GCTACCTATC CATGTGCAAG ATGCAGAAAG TGCTGCAGGA GCTCGGCTGC
    TCTCTCAAGG AGGAGGAGCT GACCAATCTT CTGAACAGTT GGGAAATCAA ATGGCATGAT
    AACTCCATCA ATTACCTTGA CTTCCTGAAA GCAGTGGAGA GCAGCAAGCC AACAAGACCT
    CAGCCAAAGA AAAAAGAAGA GAGTGTGCCA ATCAGTTTCT CAACACTGAA CCCAGAGGAG
    GTTGTAAAGA ACATCCAGGA AGTTGTTGCC TCCTCCGACA TGGCTTTGTC AACGGCATTT
    TCTGCATTGG ATAAAGAGGA CACTGGGTTT GTAAAGGCTT CAGATTTTGG ACAAGTTCTT
    AAGGATTTCT GTTACAAACT AACAGACAAC CAGTATCATT ATTTTTTGAG AAAACTGAAA
    ATTCATCTAA CCCCCCATAT AAATTGGAAA TATTTTCTCC AGAACTTCAG TTCTTTCTCT
    GAAGAGACTG CTGCCGAGTG GGCTGAGAAA ATGCCCAAAG GCCCACCGCC CACATCTCCT
    CCCAAGGAAA CAGCAAACCA GGAGCTGCTG GCTCAGCTCC ACAAGGCGGT GGGCTCCCAC
    TACCATGCGA TTGCTCAGGA GTTTGACAAC TTCGACACCA TGAAAACGTA CACCGCCTCC
    AAGGATGAAT TCAGGGCCAT TTGTGCTCGC CATGTCCAAA TTCTGACAGA TGAGCAATTT
    GACAGACTGT GGAGCAAGAT GCCAGTCAAT GCCAAGGGGA GGCTGAAGTA CCTGGACCTC
    CTGAGCATGT TCAGCTCCAA GAGAGCGCCA ACACCATTGA CCACGGGCAG CTCGGCCAAG
    GCCCAGAGAG GCAGCAGTGT TCCTGAAATC TCAGATGGAA GCAGGCCTGC AGTATCATCG
    CCCATTCATG ACCTGAAGGC GGGGTTGAAA GCACAGAGCC ACCCCTGTAC CCCCGCTAGT
    GCCAGTTCGG CGCCAGGCAC CCTGCCCTTG CAGAACTGCG AGCCCATAGA AAGCAAGCTC
    CAGAAGAAAA TCCAGGGCTG CTGGCGAGAG TTCCTGAAAG AATGCAAAGA GAAGGATGTG
    AATAAGCAGG GCCAGGTCAC CACGGCTGAA TTCCTGGCTC TTGTGGAGAA ATTCAATCTG
    GACATAAGCA AAGAGGAATG TCAGCAGCTC ATCGTGAAGT ATGATTTAAA GAACAACGGG
    AAGTTTGCTT ATTGCAACTT CATCCAGAGC TGTGTCCTCC TGTTGAAAGC GAAGGAAACC
    TCACTGATGC AGAGGATGAA AATTCAGAAT GCACATAAAA TGAAAGAATC TGGTGCTGAG
    ACTTCTTCCT TTTATTCAGC TTTACTGCGT ATCCAGCCCA AAATTGTGCA CTGCTGGAGG
    CCCATGCGGC GTACCTTCAA AGCCTATGAT GAGGGCGGGA CAGGGCTTTT AAGCGTGGCT
    GATTTCAGGA AGGTCCTGAG ACAGTACAGC ATCAACCTCT CGGAGGAGGA GTTCTTTCAC
    ATTCTGGAGT ATTATGACAA GACGCTGTCT TCCAAGATCT CCTACAACGA CTTCCTGCGG
    GCGTTCCTTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38867
    Clone ID Related Accession (Same CDS sequence) XM_014117295.1 , XM_014117295.2
    Accession Version XM_014117295.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117295.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACCTAA GTATAAATGT TATACAGAGG GGAGGTGGGA ATGAAATGAG TTGCAGTCGG 
    ACAGTAAAAG AACTTGAAAA ACAAATAGGA GAAAAGATTT TCAGAAACAT TAAAACTATT
    TTCAAAGCCT TCAAGCTCAT TGATGTTAGC AAGACTGGTC TGATCCAGTC TTGTGAACTG
    AGGAAAGTTC TCGAGACCTT CTGTTTGAAG ATGAAAGATG ATGAATACAA AAAGTTTGCA
    AAACACTACA ACATTGATAA AGATACTGCA GTGGATTACA ATGTTTTTTT GAAGAACCTC
    AGTGCAAATA ATGACTTGAA CCTTAGATAT TATATGGGAA ACCAAGATAT CTCCTGGAAG
    AATCAACCAG TCCAAAATTC CAAAGGGGAG TGCCTCCACA GTTCTGGTTC ATCTGAAGAT
    GTCTGGAAAA ACTACAACCT AGACGAAATT GAGAGAACTT TTTGCCAAGA GCTTCCCAAG
    TGCTATGAAA AGGTTGAAAA AGCCCTGAGT GCAGGGGACC CCTCCAAAAG TGGATATGTA
    TCTTTGAATT ACCTAAAGAT TGTCCTCGAC ACCTTTGTAT ACCGATTACC AAGGAGAATT
    TTTATCCAGT TAATGAAAAG ATTTGGACTT AAAACCACCA CCAAAATCAA TTGGAAACAA
    TTTCTAACAT CACTTTATGA ACCCCAGTGG TTGGAAGTCA ACAATACAGT TCCTCCAACA
    AAAAGAAATA GAATCACTTC TAGAGATCAA TCTTGCAAGG AAAACATTAT CACAAAGTTA
    TCTAGACAAG AGGATCACTA TACATCATTG AAGAAAGCAC TCCTGATCGC CAACACTAAA
    CCTGATGGAC AGATAACGGG GAAAGAATTG CGATATATTC TAAATTGCAT GGTTGTAAAA
    ATCAGTGATT CAGAATTCAA AGAGCTCATG CAAATGCTTG ACCCTGGGGA CACCGGATGG
    GTTAACATCT ACACACTTAT TGAACTGCTT GAAGAGACCC CTAAGATACA AAAACTCTCC
    TCCTCTGCAG ACACCAAGGC GCCTCTCCTC CTGGCTTGGG ATTCAGTGGA AGAAATCATT
    CAGGACTCAA TTGCTAGGAA TCTCCAAGCC TTCTACAAAA TGCTACGGTC ATATGACCTC
    GGAGACACAG GGCTCATTGG TCGAAATAAT TTCAAGAAAA TCATGCGCAT CTTCTGTCCA
    TTTTTAACAA TCGAACATTT ACTGAAACTC TGTAGTAGGT TTCAGGATAT TGCTTCAGGA
    AGAATCCTTT ATAAGAAATT TTTGGCATCC ATGGGGATGA GTGGCCCACC ACCCCCCTCT
    CCGGTTATTA CTCCAAAGGA TCAGCTATTG AGTGACCATT TTCAAAGAGA GGAGCAGCGG
    AAGTTGGATC TTTCTGAAAG AACCAAGCCC GTGGAGGACA GAAGCACCTC GACCAAGAAA
    ATGACGAAGG ATGAAGCGAT CGAAAAACTA AAAAACTGTA TTCAGCAGCG GGACCCAGCA
    TTCAAAAAAT TCTTTCTTGA CTATAGCAAG GAGCTCACTG GCAAAATTCA TGTGCGTGAC
    TTCAAGAAGG TACTGGAAGC TAGTGGAATG CCCATGGATG ATGACCAGTA CGCTCTGCTG
    ACGGCAAAAA TAGGATATAA GAGAGAAGGG ATGAGTTATC TTGATTTTGC AGCAGGATTT
    GAAGATGCTA AAATGAGCAG GCCAGAAGTG ATCCCATTGC AGACTCCAGT TCTTTCCAAG
    ACTAATTTGG ACAGATACTT TATAACCGCT GAAGAGTGCC TGAAGCAGTT CCCCCAGAGA
    CTGAAGGAAG CCTTCCGGGA CCCCTACTCT GCCTTCTTTA AAATAGACAC TGACAGGGAT
    GGCATAATCA ACATGCATGA TCTTCACAGA TTGTTACTGC ACTTACTGTT CAATCTTAAA
    GACAAAGAAT TTGAGCGTTT CCTGGGCCTT CTTGGCTTGA GACTCAGCGT CACTCTCAAT
    TTTCGGGAAT TTCGAAATCT GTGTGAGAAG AGAGCATTGA GAACAGATGA CGCGCCTCAA
    AGACTAATCA GACCAAAACA GAAGGTCGCA GATTCAGAAC TAGCTTGTGA ACAGGCTCAT
    CAGTATCTTG TTACCAAAGC AAAAACCAGA TGGTCAGACT TATCTAAGAA TTTTATAGAA
    ACTGATAATG AGGGCAATGG CATCCTTCGG CGGCGGGATA TAAAGAATGC GCTCTATGGA
    TTTGATATTC CTCTGACACC GAGGGAATTT GAAAAGCTCT GGATGAGCTA TGACACCGAG
    GGAAGAGGGC ACATAACTTA CCAAGAATTT CTACAGAAAT TGGGGATTAA CTACTCAGCA
    GACATTCATC GGCCCTATGC AGAGGATTAC TTCAACTTCA TGGGTCATTT CACAAAACCC
    CAGCAGATGC AAGAGGAGGC CAGACAGCTA GAGCAGAGCG TGGACAGGAC TGCGCCAGTT
    AGGGATAAGC TTAAGGACCA TTATCAAGAT ATCAGCAAAG CACTCACCAA ACTAGACAAA
    TCCAAAAATG GCTACCTATC CATGTGCAAG ATGCAGAAAG TGCTGCAGGA GCTCGGCTGC
    TCTCTCAAGG AGGAGGAGCT GACCAATCTT CTGAACAGTT GGGAAATCAA ATGGCATGAT
    AACTCCATCA ATTACCTTGA CTTCCTGAAA GCAGTGGAGA GCAGCAAGCC AACAAGACCT
    CAGCCAAAGA AAAAAGAAGA GAGTGTGCCA ATCAGTTTCT CAACACTGAA CCCAGAGGAG
    GTTGTAAAGA ACATCCAGGA AGTTGTTGCC TCCTCCGACA TGGCTTTGTC AACGGCATTT
    TCTGCATTGG ATAAAGAGGA CACTGGGTTT GTAAAGGCTT CAGATTTTGG ACAAGTTCTT
    AAGGATTTCT GTTACAAACT AACAGACAAC CAGTATCATT ATTTTTTGAG AAAACTGAAA
    ATTCATCTAA CCCCCCATAT AAATTGGAAA TATTTTCTCC AGAACTTCAG TTCTTTCTCT
    GAAGAGACTG CTGCCGAGTG GGCTGAGAAA ATGCCCAAAG GCCCACCGCC CACATCTCCT
    CCCAAGGAAA CAGCAAACCA GGAGCTGCTG GCTCAGCTCC ACAAGGCGGT GGGCTCCCAC
    TACCATGCGA TTGCTCAGGA GTTTGACAAC TTCGACACCA TGAAAACGTA CACCGCCTCC
    AAGGATGAAT TCAGGGCCAT TTGTGCTCGC CATGTCCAAA TTCTGACAGA TGAGCAATTT
    GACAGACTGT GGAGCAAGAT GCCAGTCAAT GCCAAGGGGA GGCTGAAGTA CCTGGACCTC
    CTGAGCATGT TCAGCTCCAA GAGAGCGCCA ACACCATTGA CCACGGGCAG CTCGGCCAAG
    GCCCAGAGAG GCAGCAGTGT TCCTGAAATC TCAGATGGAA GCAGGCCTGC AGTATCATCG
    CCCATTCATG ACCTGAAGGC GGGGTTGAAA GCACAGAGCC ACCCCTGTAC CCCCGCTAGT
    GCCAGTTCGG CGCCAGGCAC CCTGCCCTTG CAGAACTGCG AGCCCATAGA AAGCAAGCTC
    CAGAAGAAAA TCCAGGGCTG CTGGCGAGAG TTCCTGAAAG AATGCAAAGA GAAGGATGTG
    AATAAGCAGG GCCAGGTCAC CACGGCTGAA TTCCTGGCTC TTGTGGAGAA ATTCAATCTG
    GACATAAGCA AAGAGGAATG TCAGCAGCTC ATCGTGAAGT ATGATTTAAA GAACAACGGG
    AAGTTTGCTT ATTGCAACTT CATCCAGAGC TGTGTCCTCC TGTTGAAAGC GAAGGAAACC
    TCACTGATGC AGAGGATGAA AATTCAGAAT GCACATAAAA TGAAAGAATC TGGTGCTGAG
    ACTTCTTCCT TTTATTCAGC TTTACTGCGT ATCCAGCCCA AAATTGTGCA CTGCTGGAGG
    CCCATGCGGC GTACCTTCAA AGCCTATGAT GAGGGCGGGA CAGGGCTTTT AAGCGTGGCT
    GATTTCAGGA AGGTCCTGAG ACAGTACAGC ATCAACCTCT CGGAGGAGGA GTTCTTTCAC
    ATTCTGGAGT ATTATGACAA GACGCTGTCT TCCAAGATCT CCTACAACGA CTTCCTGCGG
    GCGTTCCTTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972770.1
    CDS200..4294
    Translation

    Target ORF information:

    RefSeq Version XM_014117295.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117295.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACCTAA GTATAAATGT TATACAGAGG GGAGGTGGGA ATGAAATGAG TTGCAGTCGG 
    ACAGTAAAAG AACTTGAAAA ACAAATAGGA GAAAAGATTT TCAGAAACAT TAAAACTATT
    TTCAAAGCCT TCAAGCTCAT TGATGTTAGC AAGACTGGTC TGATCCAGTC TTGTGAACTG
    AGGAAAGTTC TCGAGACCTT CTGTTTGAAG ATGAAAGATG ATGAATACAA AAAGTTTGCA
    AAACACTACA ACATTGATAA AGATACTGCA GTGGATTACA ATGTTTTTTT GAAGAACCTC
    AGTGCAAATA ATGACTTGAA CCTTAGATAT TATATGGGAA ACCAAGATAT CTCCTGGAAG
    AATCAACCAG TCCAAAATTC CAAAGGGGAG TGCCTCCACA GTTCTGGTTC ATCTGAAGAT
    GTCTGGAAAA ACTACAACCT AGACGAAATT GAGAGAACTT TTTGCCAAGA GCTTCCCAAG
    TGCTATGAAA AGGTTGAAAA AGCCCTGAGT GCAGGGGACC CCTCCAAAAG TGGATATGTA
    TCTTTGAATT ACCTAAAGAT TGTCCTCGAC ACCTTTGTAT ACCGATTACC AAGGAGAATT
    TTTATCCAGT TAATGAAAAG ATTTGGACTT AAAACCACCA CCAAAATCAA TTGGAAACAA
    TTTCTAACAT CACTTTATGA ACCCCAGTGG TTGGAAGTCA ACAATACAGT TCCTCCAACA
    AAAAGAAATA GAATCACTTC TAGAGATCAA TCTTGCAAGG AAAACATTAT CACAAAGTTA
    TCTAGACAAG AGGATCACTA TACATCATTG AAGAAAGCAC TCCTGATCGC CAACACTAAA
    CCTGATGGAC AGATAACGGG GAAAGAATTG CGATATATTC TAAATTGCAT GGTTGTAAAA
    ATCAGTGATT CAGAATTCAA AGAGCTCATG CAAATGCTTG ACCCTGGGGA CACCGGATGG
    GTTAACATCT ACACACTTAT TGAACTGCTT GAAGAGACCC CTAAGATACA AAAACTCTCC
    TCCTCTGCAG ACACCAAGGC GCCTCTCCTC CTGGCTTGGG ATTCAGTGGA AGAAATCATT
    CAGGACTCAA TTGCTAGGAA TCTCCAAGCC TTCTACAAAA TGCTACGGTC ATATGACCTC
    GGAGACACAG GGCTCATTGG TCGAAATAAT TTCAAGAAAA TCATGCGCAT CTTCTGTCCA
    TTTTTAACAA TCGAACATTT ACTGAAACTC TGTAGTAGGT TTCAGGATAT TGCTTCAGGA
    AGAATCCTTT ATAAGAAATT TTTGGCATCC ATGGGGATGA GTGGCCCACC ACCCCCCTCT
    CCGGTTATTA CTCCAAAGGA TCAGCTATTG AGTGACCATT TTCAAAGAGA GGAGCAGCGG
    AAGTTGGATC TTTCTGAAAG AACCAAGCCC GTGGAGGACA GAAGCACCTC GACCAAGAAA
    ATGACGAAGG ATGAAGCGAT CGAAAAACTA AAAAACTGTA TTCAGCAGCG GGACCCAGCA
    TTCAAAAAAT TCTTTCTTGA CTATAGCAAG GAGCTCACTG GCAAAATTCA TGTGCGTGAC
    TTCAAGAAGG TACTGGAAGC TAGTGGAATG CCCATGGATG ATGACCAGTA CGCTCTGCTG
    ACGGCAAAAA TAGGATATAA GAGAGAAGGG ATGAGTTATC TTGATTTTGC AGCAGGATTT
    GAAGATGCTA AAATGAGCAG GCCAGAAGTG ATCCCATTGC AGACTCCAGT TCTTTCCAAG
    ACTAATTTGG ACAGATACTT TATAACCGCT GAAGAGTGCC TGAAGCAGTT CCCCCAGAGA
    CTGAAGGAAG CCTTCCGGGA CCCCTACTCT GCCTTCTTTA AAATAGACAC TGACAGGGAT
    GGCATAATCA ACATGCATGA TCTTCACAGA TTGTTACTGC ACTTACTGTT CAATCTTAAA
    GACAAAGAAT TTGAGCGTTT CCTGGGCCTT CTTGGCTTGA GACTCAGCGT CACTCTCAAT
    TTTCGGGAAT TTCGAAATCT GTGTGAGAAG AGAGCATTGA GAACAGATGA CGCGCCTCAA
    AGACTAATCA GACCAAAACA GAAGGTCGCA GATTCAGAAC TAGCTTGTGA ACAGGCTCAT
    CAGTATCTTG TTACCAAAGC AAAAACCAGA TGGTCAGACT TATCTAAGAA TTTTATAGAA
    ACTGATAATG AGGGCAATGG CATCCTTCGG CGGCGGGATA TAAAGAATGC GCTCTATGGA
    TTTGATATTC CTCTGACACC GAGGGAATTT GAAAAGCTCT GGATGAGCTA TGACACCGAG
    GGAAGAGGGC ACATAACTTA CCAAGAATTT CTACAGAAAT TGGGGATTAA CTACTCAGCA
    GACATTCATC GGCCCTATGC AGAGGATTAC TTCAACTTCA TGGGTCATTT CACAAAACCC
    CAGCAGATGC AAGAGGAGGC CAGACAGCTA GAGCAGAGCG TGGACAGGAC TGCGCCAGTT
    AGGGATAAGC TTAAGGACCA TTATCAAGAT ATCAGCAAAG CACTCACCAA ACTAGACAAA
    TCCAAAAATG GCTACCTATC CATGTGCAAG ATGCAGAAAG TGCTGCAGGA GCTCGGCTGC
    TCTCTCAAGG AGGAGGAGCT GACCAATCTT CTGAACAGTT GGGAAATCAA ATGGCATGAT
    AACTCCATCA ATTACCTTGA CTTCCTGAAA GCAGTGGAGA GCAGCAAGCC AACAAGACCT
    CAGCCAAAGA AAAAAGAAGA GAGTGTGCCA ATCAGTTTCT CAACACTGAA CCCAGAGGAG
    GTTGTAAAGA ACATCCAGGA AGTTGTTGCC TCCTCCGACA TGGCTTTGTC AACGGCATTT
    TCTGCATTGG ATAAAGAGGA CACTGGGTTT GTAAAGGCTT CAGATTTTGG ACAAGTTCTT
    AAGGATTTCT GTTACAAACT AACAGACAAC CAGTATCATT ATTTTTTGAG AAAACTGAAA
    ATTCATCTAA CCCCCCATAT AAATTGGAAA TATTTTCTCC AGAACTTCAG TTCTTTCTCT
    GAAGAGACTG CTGCCGAGTG GGCTGAGAAA ATGCCCAAAG GCCCACCGCC CACATCTCCT
    CCCAAGGAAA CAGCAAACCA GGAGCTGCTG GCTCAGCTCC ACAAGGCGGT GGGCTCCCAC
    TACCATGCGA TTGCTCAGGA GTTTGACAAC TTCGACACCA TGAAAACGTA CACCGCCTCC
    AAGGATGAAT TCAGGGCCAT TTGTGCTCGC CATGTCCAAA TTCTGACAGA TGAGCAATTT
    GACAGACTGT GGAGCAAGAT GCCAGTCAAT GCCAAGGGGA GGCTGAAGTA CCTGGACCTC
    CTGAGCATGT TCAGCTCCAA GAGAGCGCCA ACACCATTGA CCACGGGCAG CTCGGCCAAG
    GCCCAGAGAG GCAGCAGTGT TCCTGAAATC TCAGATGGAA GCAGGCCTGC AGTATCATCG
    CCCATTCATG ACCTGAAGGC GGGGTTGAAA GCACAGAGCC ACCCCTGTAC CCCCGCTAGT
    GCCAGTTCGG CGCCAGGCAC CCTGCCCTTG CAGAACTGCG AGCCCATAGA AAGCAAGCTC
    CAGAAGAAAA TCCAGGGCTG CTGGCGAGAG TTCCTGAAAG AATGCAAAGA GAAGGATGTG
    AATAAGCAGG GCCAGGTCAC CACGGCTGAA TTCCTGGCTC TTGTGGAGAA ATTCAATCTG
    GACATAAGCA AAGAGGAATG TCAGCAGCTC ATCGTGAAGT ATGATTTAAA GAACAACGGG
    AAGTTTGCTT ATTGCAACTT CATCCAGAGC TGTGTCCTCC TGTTGAAAGC GAAGGAAACC
    TCACTGATGC AGAGGATGAA AATTCAGAAT GCACATAAAA TGAAAGAATC TGGTGCTGAG
    ACTTCTTCCT TTTATTCAGC TTTACTGCGT ATCCAGCCCA AAATTGTGCA CTGCTGGAGG
    CCCATGCGGC GTACCTTCAA AGCCTATGAT GAGGGCGGGA CAGGGCTTTT AAGCGTGGCT
    GATTTCAGGA AGGTCCTGAG ACAGTACAGC ATCAACCTCT CGGAGGAGGA GTTCTTTCAC
    ATTCTGGAGT ATTATGACAA GACGCTGTCT TCCAAGATCT CCTACAACGA CTTCCTGCGG
    GCGTTCCTTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38868
    Clone ID Related Accession (Same CDS sequence) XM_014117296.1 , XM_022424333.1
    Accession Version XM_014117296.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAGATG ATGAATACAA AAAGTTTGCA AAACACTACA ACATTGATAA AGATACTGCA 
    GTGGATTACA ATGTTTTTTT GAAGAACCTC AGTGCAAATA ATGACTTGAA CCTTAGATAT
    TATATGGGAA ACCAAGATAT CTCCTGGAAG AATCAACCAG TCCAAAATTC CAAAGGGGAG
    TGCCTCCACA GTTCTGGTTC ATCTGAAGAT GTCTGGAAAA ACTACAACCT AGACGAAATT
    GAGAGAACTT TTTGCCAAGA GCTTCCCAAG TGCTATGAAA AGGTTGAAAA AGCCCTGAGT
    GCAGGGGACC CCTCCAAAAG TGGATATGTA TCTTTGAATT ACCTAAAGAT TGTCCTCGAC
    ACCTTTGTAT ACCGATTACC AAGGAGAATT TTTATCCAGT TAATGAAAAG ATTTGGACTT
    AAAACCACCA CCAAAATCAA TTGGAAACAA TTTCTAACAT CACTTTATGA ACCCCAGTGG
    TTGGAAGTCA ACAATACAGT TCCTCCAACA AAAAGAAATA GAATCACTTC TAGAGATCAA
    TCTTGCAAGG AAAACATTAT CACAAAGTTA TCTAGACAAG AGGATCACTA TACATCATTG
    AAGAAAGCAC TCCTGATCGC CAACACTAAA CCTGATGGAC AGATAACGGG GAAAGAATTG
    CGATATATTC TAAATTGCAT GGTTGTAAAA ATCAGTGATT CAGAATTCAA AGAGCTCATG
    CAAATGCTTG ACCCTGGGGA CACCGGATGG GTTAACATCT ACACACTTAT TGAACTGCTT
    GAAGAGACCC CTAAGATACA AAAACTCTCC TCCTCTGCAG ACACCAAGGC GCCTCTCCTC
    CTGGCTTGGG ATTCAGTGGA AGAAATCATT CAGGACTCAA TTGCTAGGAA TCTCCAAGCC
    TTCTACAAAA TGCTACGGTC ATATGACCTC GGAGACACAG GGCTCATTGG TCGAAATAAT
    TTCAAGAAAA TCATGCGCAT CTTCTGTCCA TTTTTAACAA TCGAACATTT ACTGAAACTC
    TGTAGTAGGT TTCAGGATAT TGCTTCAGGA AGAATCCTTT ATAAGAAATT TTTGGCATCC
    ATGGGGATGA GTGGCCCACC ACCCCCCTCT CCGGTTATTA CTCCAAAGGA TCAGCTATTG
    AGTGACCATT TTCAAAGAGA GGAGCAGCGG AAGTTGGATC TTTCTGAAAG AACCAAGCCC
    GTGGAGGACA GAAGCACCTC GACCAAGAAA ATGACGAAGG ATGAAGCGAT CGAAAAACTA
    AAAAACTGTA TTCAGCAGCG GGACCCAGCA TTCAAAAAAT TCTTTCTTGA CTATAGCAAG
    GAGCTCACTG GCAAAATTCA TGTGCGTGAC TTCAAGAAGG TACTGGAAGC TAGTGGAATG
    CCCATGGATG ATGACCAGTA CGCTCTGCTG ACGGCAAAAA TAGGATATAA GAGAGAAGGG
    ATGAGTTATC TTGATTTTGC AGCAGGATTT GAAGATGCTA AAATGAGCAG GCCAGAAGTG
    ATCCCATTGC AGACTCCAGT TCTTTCCAAG ACTAATTTGG ACAGATACTT TATAACCGCT
    GAAGAGTGCC TGAAGCAGTT CCCCCAGAGA CTGAAGGAAG CCTTCCGGGA CCCCTACTCT
    GCCTTCTTTA AAATAGACAC TGACAGGGAT GGCATAATCA ACATGCATGA TCTTCACAGA
    TTGTTACTGC ACTTACTGTT CAATCTTAAA GACAAAGAAT TTGAGCGTTT CCTGGGCCTT
    CTTGGCTTGA GACTCAGCGT CACTCTCAAT TTTCGGGAAT TTCGAAATCT GTGTGAGAAG
    AGAGCATTGA GAACAGATGA CGCGCCTCAA AGACTAATCA GACCAAAACA GAAGGTCGCA
    GATTCAGAAC TAGCTTGTGA ACAGGCTCAT CAGTATCTTG TTACCAAAGC AAAAACCAGA
    TGGTCAGACT TATCTAAGAA TTTTATAGAA ACTGATAATG AGGGCAATGG CATCCTTCGG
    CGGCGGGATA TAAAGAATGC GCTCTATGGA TTTGATATTC CTCTGACACC GAGGGAATTT
    GAAAAGCTCT GGATGAGCTA TGACACCGAG GGAAGAGGGC ACATAACTTA CCAAGAATTT
    CTACAGAAAT TGGGGATTAA CTACTCAGCA GACATTCATC GGCCCTATGC AGAGGATTAC
    TTCAACTTCA TGGGTCATTT CACAAAACCC CAGCAGATGC AAGAGGAGGC CAGACAGCTA
    GAGCAGAGCG TGGACAGGAC TGCGCCAGTT AGGGATAAGC TTAAGGACCA TTATCAAGAT
    ATCAGCAAAG CACTCACCAA ACTAGACAAA TCCAAAAATG GCTACCTATC CATGTGCAAG
    ATGCAGAAAG TGCTGCAGGA GCTCGGCTGC TCTCTCAAGG AGGAGGAGCT GACCAATCTT
    CTGAACAGTT GGGAAATCAA ATGGCATGAT AACTCCATCA ATTACCTTGA CTTCCTGAAA
    GCAGTGGAGA GCAGCAAGCC AACAAGACCT CAGCCAAAGA AAAAAGAAGA GAGTGTGCCA
    ATCAGTTTCT CAACACTGAA CCCAGAGGAG GTTGTAAAGA ACATCCAGGA AGTTGTTGCC
    TCCTCCGACA TGGCTTTGTC AACGGCATTT TCTGCATTGG ATAAAGAGGA CACTGGGTTT
    GTAAAGGCTT CAGATTTTGG ACAAGTTCTT AAGGATTTCT GTTACAAACT AACAGACAAC
    CAGTATCATT ATTTTTTGAG AAAACTGAAA ATTCATCTAA CCCCCCATAT AAATTGGAAA
    TATTTTCTCC AGAACTTCAG TTCTTTCTCT GAAGAGACTG CTGCCGAGTG GGCTGAGAAA
    ATGCCCAAAG GCCCACCGCC CACATCTCCT CCCAAGGAAA CAGCAAACCA GGAGCTGCTG
    GCTCAGCTCC ACAAGGCGGT GGGCTCCCAC TACCATGCGA TTGCTCAGGA GTTTGACAAC
    TTCGACACCA TGAAAACGTA CACCGCCTCC AAGGATGAAT TCAGGGCCAT TTGTGCTCGC
    CATGTCCAAA TTCTGACAGA TGAGCAATTT GACAGACTGT GGAGCAAGAT GCCAGTCAAT
    GCCAAGGGGA GGCTGAAGTA CCTGGACCTC CTGAGCATGT TCAGCTCCAA GAGAGCGCCA
    ACACCATTGA CCACGGGCAG CTCGGCCAAG GCCCAGAGAG GCAGCAGTGT TCCTGAAATC
    TCAGATGGAA GCAGGCCTGC AGTATCATCG CCCATTCATG ACCTGAAGGC GGGGTTGAAA
    GCACAGAGCC ACCCCTGTAC CCCCGCTAGT GCCAGTTCGG CGCCAGGCAC CCTGCCCTTG
    CAGAACTGCG AGCCCATAGA AAGCAAGCTC CAGAAGAAAA TCCAGGGCTG CTGGCGAGAG
    TTCCTGAAAG AATGCAAAGA GAAGGATGTG AATAAGCAGG GCCAGGTCAC CACGGCTGAA
    TTCCTGGCTC TTGTGGAGAA ATTCAATCTG GACATAAGCA AAGAGGAATG TCAGCAGCTC
    ATCGTGAAGT ATGATTTAAA GAACAACGGG AAGTTTGCTT ATTGCAACTT CATCCAGAGC
    TGTGTCCTCC TGTTGAAAGC GAAGGAAACC TCACTGATGC AGAGGATGAA AATTCAGAAT
    GCACATAAAA TGAAAGAATC TGGTGCTGAG ACTTCTTCCT TTTATTCAGC TTTACTGCGT
    ATCCAGCCCA AAATTGTGCA CTGCTGGAGG CCCATGCGGC GTACCTTCAA AGCCTATGAT
    GAGGGCGGGA CAGGGCTTTT AAGCGTGGCT GATTTCAGGA AGGTCCTGAG ACAGTACAGC
    ATCAACCTCT CGGAGGAGGA GTTCTTTCAC ATTCTGGAGT ATTATGACAA GACGCTGTCT
    TCCAAGATCT CCTACAACGA CTTCCTGCGG GCGTTCCTTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972771.1
    CDS284..4168
    Translation

    Target ORF information:

    RefSeq Version XM_014117296.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117296.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAGATG ATGAATACAA AAAGTTTGCA AAACACTACA ACATTGATAA AGATACTGCA 
    GTGGATTACA ATGTTTTTTT GAAGAACCTC AGTGCAAATA ATGACTTGAA CCTTAGATAT
    TATATGGGAA ACCAAGATAT CTCCTGGAAG AATCAACCAG TCCAAAATTC CAAAGGGGAG
    TGCCTCCACA GTTCTGGTTC ATCTGAAGAT GTCTGGAAAA ACTACAACCT AGACGAAATT
    GAGAGAACTT TTTGCCAAGA GCTTCCCAAG TGCTATGAAA AGGTTGAAAA AGCCCTGAGT
    GCAGGGGACC CCTCCAAAAG TGGATATGTA TCTTTGAATT ACCTAAAGAT TGTCCTCGAC
    ACCTTTGTAT ACCGATTACC AAGGAGAATT TTTATCCAGT TAATGAAAAG ATTTGGACTT
    AAAACCACCA CCAAAATCAA TTGGAAACAA TTTCTAACAT CACTTTATGA ACCCCAGTGG
    TTGGAAGTCA ACAATACAGT TCCTCCAACA AAAAGAAATA GAATCACTTC TAGAGATCAA
    TCTTGCAAGG AAAACATTAT CACAAAGTTA TCTAGACAAG AGGATCACTA TACATCATTG
    AAGAAAGCAC TCCTGATCGC CAACACTAAA CCTGATGGAC AGATAACGGG GAAAGAATTG
    CGATATATTC TAAATTGCAT GGTTGTAAAA ATCAGTGATT CAGAATTCAA AGAGCTCATG
    CAAATGCTTG ACCCTGGGGA CACCGGATGG GTTAACATCT ACACACTTAT TGAACTGCTT
    GAAGAGACCC CTAAGATACA AAAACTCTCC TCCTCTGCAG ACACCAAGGC GCCTCTCCTC
    CTGGCTTGGG ATTCAGTGGA AGAAATCATT CAGGACTCAA TTGCTAGGAA TCTCCAAGCC
    TTCTACAAAA TGCTACGGTC ATATGACCTC GGAGACACAG GGCTCATTGG TCGAAATAAT
    TTCAAGAAAA TCATGCGCAT CTTCTGTCCA TTTTTAACAA TCGAACATTT ACTGAAACTC
    TGTAGTAGGT TTCAGGATAT TGCTTCAGGA AGAATCCTTT ATAAGAAATT TTTGGCATCC
    ATGGGGATGA GTGGCCCACC ACCCCCCTCT CCGGTTATTA CTCCAAAGGA TCAGCTATTG
    AGTGACCATT TTCAAAGAGA GGAGCAGCGG AAGTTGGATC TTTCTGAAAG AACCAAGCCC
    GTGGAGGACA GAAGCACCTC GACCAAGAAA ATGACGAAGG ATGAAGCGAT CGAAAAACTA
    AAAAACTGTA TTCAGCAGCG GGACCCAGCA TTCAAAAAAT TCTTTCTTGA CTATAGCAAG
    GAGCTCACTG GCAAAATTCA TGTGCGTGAC TTCAAGAAGG TACTGGAAGC TAGTGGAATG
    CCCATGGATG ATGACCAGTA CGCTCTGCTG ACGGCAAAAA TAGGATATAA GAGAGAAGGG
    ATGAGTTATC TTGATTTTGC AGCAGGATTT GAAGATGCTA AAATGAGCAG GCCAGAAGTG
    ATCCCATTGC AGACTCCAGT TCTTTCCAAG ACTAATTTGG ACAGATACTT TATAACCGCT
    GAAGAGTGCC TGAAGCAGTT CCCCCAGAGA CTGAAGGAAG CCTTCCGGGA CCCCTACTCT
    GCCTTCTTTA AAATAGACAC TGACAGGGAT GGCATAATCA ACATGCATGA TCTTCACAGA
    TTGTTACTGC ACTTACTGTT CAATCTTAAA GACAAAGAAT TTGAGCGTTT CCTGGGCCTT
    CTTGGCTTGA GACTCAGCGT CACTCTCAAT TTTCGGGAAT TTCGAAATCT GTGTGAGAAG
    AGAGCATTGA GAACAGATGA CGCGCCTCAA AGACTAATCA GACCAAAACA GAAGGTCGCA
    GATTCAGAAC TAGCTTGTGA ACAGGCTCAT CAGTATCTTG TTACCAAAGC AAAAACCAGA
    TGGTCAGACT TATCTAAGAA TTTTATAGAA ACTGATAATG AGGGCAATGG CATCCTTCGG
    CGGCGGGATA TAAAGAATGC GCTCTATGGA TTTGATATTC CTCTGACACC GAGGGAATTT
    GAAAAGCTCT GGATGAGCTA TGACACCGAG GGAAGAGGGC ACATAACTTA CCAAGAATTT
    CTACAGAAAT TGGGGATTAA CTACTCAGCA GACATTCATC GGCCCTATGC AGAGGATTAC
    TTCAACTTCA TGGGTCATTT CACAAAACCC CAGCAGATGC AAGAGGAGGC CAGACAGCTA
    GAGCAGAGCG TGGACAGGAC TGCGCCAGTT AGGGATAAGC TTAAGGACCA TTATCAAGAT
    ATCAGCAAAG CACTCACCAA ACTAGACAAA TCCAAAAATG GCTACCTATC CATGTGCAAG
    ATGCAGAAAG TGCTGCAGGA GCTCGGCTGC TCTCTCAAGG AGGAGGAGCT GACCAATCTT
    CTGAACAGTT GGGAAATCAA ATGGCATGAT AACTCCATCA ATTACCTTGA CTTCCTGAAA
    GCAGTGGAGA GCAGCAAGCC AACAAGACCT CAGCCAAAGA AAAAAGAAGA GAGTGTGCCA
    ATCAGTTTCT CAACACTGAA CCCAGAGGAG GTTGTAAAGA ACATCCAGGA AGTTGTTGCC
    TCCTCCGACA TGGCTTTGTC AACGGCATTT TCTGCATTGG ATAAAGAGGA CACTGGGTTT
    GTAAAGGCTT CAGATTTTGG ACAAGTTCTT AAGGATTTCT GTTACAAACT AACAGACAAC
    CAGTATCATT ATTTTTTGAG AAAACTGAAA ATTCATCTAA CCCCCCATAT AAATTGGAAA
    TATTTTCTCC AGAACTTCAG TTCTTTCTCT GAAGAGACTG CTGCCGAGTG GGCTGAGAAA
    ATGCCCAAAG GCCCACCGCC CACATCTCCT CCCAAGGAAA CAGCAAACCA GGAGCTGCTG
    GCTCAGCTCC ACAAGGCGGT GGGCTCCCAC TACCATGCGA TTGCTCAGGA GTTTGACAAC
    TTCGACACCA TGAAAACGTA CACCGCCTCC AAGGATGAAT TCAGGGCCAT TTGTGCTCGC
    CATGTCCAAA TTCTGACAGA TGAGCAATTT GACAGACTGT GGAGCAAGAT GCCAGTCAAT
    GCCAAGGGGA GGCTGAAGTA CCTGGACCTC CTGAGCATGT TCAGCTCCAA GAGAGCGCCA
    ACACCATTGA CCACGGGCAG CTCGGCCAAG GCCCAGAGAG GCAGCAGTGT TCCTGAAATC
    TCAGATGGAA GCAGGCCTGC AGTATCATCG CCCATTCATG ACCTGAAGGC GGGGTTGAAA
    GCACAGAGCC ACCCCTGTAC CCCCGCTAGT GCCAGTTCGG CGCCAGGCAC CCTGCCCTTG
    CAGAACTGCG AGCCCATAGA AAGCAAGCTC CAGAAGAAAA TCCAGGGCTG CTGGCGAGAG
    TTCCTGAAAG AATGCAAAGA GAAGGATGTG AATAAGCAGG GCCAGGTCAC CACGGCTGAA
    TTCCTGGCTC TTGTGGAGAA ATTCAATCTG GACATAAGCA AAGAGGAATG TCAGCAGCTC
    ATCGTGAAGT ATGATTTAAA GAACAACGGG AAGTTTGCTT ATTGCAACTT CATCCAGAGC
    TGTGTCCTCC TGTTGAAAGC GAAGGAAACC TCACTGATGC AGAGGATGAA AATTCAGAAT
    GCACATAAAA TGAAAGAATC TGGTGCTGAG ACTTCTTCCT TTTATTCAGC TTTACTGCGT
    ATCCAGCCCA AAATTGTGCA CTGCTGGAGG CCCATGCGGC GTACCTTCAA AGCCTATGAT
    GAGGGCGGGA CAGGGCTTTT AAGCGTGGCT GATTTCAGGA AGGTCCTGAG ACAGTACAGC
    ATCAACCTCT CGGAGGAGGA GTTCTTTCAC ATTCTGGAGT ATTATGACAA GACGCTGTCT
    TCCAAGATCT CCTACAACGA CTTCCTGCGG GCGTTCCTTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe38868
    Clone ID Related Accession (Same CDS sequence) XM_014117296.1 , XM_022424333.1
    Accession Version XM_022424333.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAGATG ATGAATACAA AAAGTTTGCA AAACACTACA ACATTGATAA AGATACTGCA 
    GTGGATTACA ATGTTTTTTT GAAGAACCTC AGTGCAAATA ATGACTTGAA CCTTAGATAT
    TATATGGGAA ACCAAGATAT CTCCTGGAAG AATCAACCAG TCCAAAATTC CAAAGGGGAG
    TGCCTCCACA GTTCTGGTTC ATCTGAAGAT GTCTGGAAAA ACTACAACCT AGACGAAATT
    GAGAGAACTT TTTGCCAAGA GCTTCCCAAG TGCTATGAAA AGGTTGAAAA AGCCCTGAGT
    GCAGGGGACC CCTCCAAAAG TGGATATGTA TCTTTGAATT ACCTAAAGAT TGTCCTCGAC
    ACCTTTGTAT ACCGATTACC AAGGAGAATT TTTATCCAGT TAATGAAAAG ATTTGGACTT
    AAAACCACCA CCAAAATCAA TTGGAAACAA TTTCTAACAT CACTTTATGA ACCCCAGTGG
    TTGGAAGTCA ACAATACAGT TCCTCCAACA AAAAGAAATA GAATCACTTC TAGAGATCAA
    TCTTGCAAGG AAAACATTAT CACAAAGTTA TCTAGACAAG AGGATCACTA TACATCATTG
    AAGAAAGCAC TCCTGATCGC CAACACTAAA CCTGATGGAC AGATAACGGG GAAAGAATTG
    CGATATATTC TAAATTGCAT GGTTGTAAAA ATCAGTGATT CAGAATTCAA AGAGCTCATG
    CAAATGCTTG ACCCTGGGGA CACCGGATGG GTTAACATCT ACACACTTAT TGAACTGCTT
    GAAGAGACCC CTAAGATACA AAAACTCTCC TCCTCTGCAG ACACCAAGGC GCCTCTCCTC
    CTGGCTTGGG ATTCAGTGGA AGAAATCATT CAGGACTCAA TTGCTAGGAA TCTCCAAGCC
    TTCTACAAAA TGCTACGGTC ATATGACCTC GGAGACACAG GGCTCATTGG TCGAAATAAT
    TTCAAGAAAA TCATGCGCAT CTTCTGTCCA TTTTTAACAA TCGAACATTT ACTGAAACTC
    TGTAGTAGGT TTCAGGATAT TGCTTCAGGA AGAATCCTTT ATAAGAAATT TTTGGCATCC
    ATGGGGATGA GTGGCCCACC ACCCCCCTCT CCGGTTATTA CTCCAAAGGA TCAGCTATTG
    AGTGACCATT TTCAAAGAGA GGAGCAGCGG AAGTTGGATC TTTCTGAAAG AACCAAGCCC
    GTGGAGGACA GAAGCACCTC GACCAAGAAA ATGACGAAGG ATGAAGCGAT CGAAAAACTA
    AAAAACTGTA TTCAGCAGCG GGACCCAGCA TTCAAAAAAT TCTTTCTTGA CTATAGCAAG
    GAGCTCACTG GCAAAATTCA TGTGCGTGAC TTCAAGAAGG TACTGGAAGC TAGTGGAATG
    CCCATGGATG ATGACCAGTA CGCTCTGCTG ACGGCAAAAA TAGGATATAA GAGAGAAGGG
    ATGAGTTATC TTGATTTTGC AGCAGGATTT GAAGATGCTA AAATGAGCAG GCCAGAAGTG
    ATCCCATTGC AGACTCCAGT TCTTTCCAAG ACTAATTTGG ACAGATACTT TATAACCGCT
    GAAGAGTGCC TGAAGCAGTT CCCCCAGAGA CTGAAGGAAG CCTTCCGGGA CCCCTACTCT
    GCCTTCTTTA AAATAGACAC TGACAGGGAT GGCATAATCA ACATGCATGA TCTTCACAGA
    TTGTTACTGC ACTTACTGTT CAATCTTAAA GACAAAGAAT TTGAGCGTTT CCTGGGCCTT
    CTTGGCTTGA GACTCAGCGT CACTCTCAAT TTTCGGGAAT TTCGAAATCT GTGTGAGAAG
    AGAGCATTGA GAACAGATGA CGCGCCTCAA AGACTAATCA GACCAAAACA GAAGGTCGCA
    GATTCAGAAC TAGCTTGTGA ACAGGCTCAT CAGTATCTTG TTACCAAAGC AAAAACCAGA
    TGGTCAGACT TATCTAAGAA TTTTATAGAA ACTGATAATG AGGGCAATGG CATCCTTCGG
    CGGCGGGATA TAAAGAATGC GCTCTATGGA TTTGATATTC CTCTGACACC GAGGGAATTT
    GAAAAGCTCT GGATGAGCTA TGACACCGAG GGAAGAGGGC ACATAACTTA CCAAGAATTT
    CTACAGAAAT TGGGGATTAA CTACTCAGCA GACATTCATC GGCCCTATGC AGAGGATTAC
    TTCAACTTCA TGGGTCATTT CACAAAACCC CAGCAGATGC AAGAGGAGGC CAGACAGCTA
    GAGCAGAGCG TGGACAGGAC TGCGCCAGTT AGGGATAAGC TTAAGGACCA TTATCAAGAT
    ATCAGCAAAG CACTCACCAA ACTAGACAAA TCCAAAAATG GCTACCTATC CATGTGCAAG
    ATGCAGAAAG TGCTGCAGGA GCTCGGCTGC TCTCTCAAGG AGGAGGAGCT GACCAATCTT
    CTGAACAGTT GGGAAATCAA ATGGCATGAT AACTCCATCA ATTACCTTGA CTTCCTGAAA
    GCAGTGGAGA GCAGCAAGCC AACAAGACCT CAGCCAAAGA AAAAAGAAGA GAGTGTGCCA
    ATCAGTTTCT CAACACTGAA CCCAGAGGAG GTTGTAAAGA ACATCCAGGA AGTTGTTGCC
    TCCTCCGACA TGGCTTTGTC AACGGCATTT TCTGCATTGG ATAAAGAGGA CACTGGGTTT
    GTAAAGGCTT CAGATTTTGG ACAAGTTCTT AAGGATTTCT GTTACAAACT AACAGACAAC
    CAGTATCATT ATTTTTTGAG AAAACTGAAA ATTCATCTAA CCCCCCATAT AAATTGGAAA
    TATTTTCTCC AGAACTTCAG TTCTTTCTCT GAAGAGACTG CTGCCGAGTG GGCTGAGAAA
    ATGCCCAAAG GCCCACCGCC CACATCTCCT CCCAAGGAAA CAGCAAACCA GGAGCTGCTG
    GCTCAGCTCC ACAAGGCGGT GGGCTCCCAC TACCATGCGA TTGCTCAGGA GTTTGACAAC
    TTCGACACCA TGAAAACGTA CACCGCCTCC AAGGATGAAT TCAGGGCCAT TTGTGCTCGC
    CATGTCCAAA TTCTGACAGA TGAGCAATTT GACAGACTGT GGAGCAAGAT GCCAGTCAAT
    GCCAAGGGGA GGCTGAAGTA CCTGGACCTC CTGAGCATGT TCAGCTCCAA GAGAGCGCCA
    ACACCATTGA CCACGGGCAG CTCGGCCAAG GCCCAGAGAG GCAGCAGTGT TCCTGAAATC
    TCAGATGGAA GCAGGCCTGC AGTATCATCG CCCATTCATG ACCTGAAGGC GGGGTTGAAA
    GCACAGAGCC ACCCCTGTAC CCCCGCTAGT GCCAGTTCGG CGCCAGGCAC CCTGCCCTTG
    CAGAACTGCG AGCCCATAGA AAGCAAGCTC CAGAAGAAAA TCCAGGGCTG CTGGCGAGAG
    TTCCTGAAAG AATGCAAAGA GAAGGATGTG AATAAGCAGG GCCAGGTCAC CACGGCTGAA
    TTCCTGGCTC TTGTGGAGAA ATTCAATCTG GACATAAGCA AAGAGGAATG TCAGCAGCTC
    ATCGTGAAGT ATGATTTAAA GAACAACGGG AAGTTTGCTT ATTGCAACTT CATCCAGAGC
    TGTGTCCTCC TGTTGAAAGC GAAGGAAACC TCACTGATGC AGAGGATGAA AATTCAGAAT
    GCACATAAAA TGAAAGAATC TGGTGCTGAG ACTTCTTCCT TTTATTCAGC TTTACTGCGT
    ATCCAGCCCA AAATTGTGCA CTGCTGGAGG CCCATGCGGC GTACCTTCAA AGCCTATGAT
    GAGGGCGGGA CAGGGCTTTT AAGCGTGGCT GATTTCAGGA AGGTCCTGAG ACAGTACAGC
    ATCAACCTCT CGGAGGAGGA GTTCTTTCAC ATTCTGGAGT ATTATGACAA GACGCTGTCT
    TCCAAGATCT CCTACAACGA CTTCCTGCGG GCGTTCCTTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280041.1
    CDS222..4106
    Translation

    Target ORF information:

    RefSeq Version XM_022424333.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424333.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAAGATG ATGAATACAA AAAGTTTGCA AAACACTACA ACATTGATAA AGATACTGCA 
    GTGGATTACA ATGTTTTTTT GAAGAACCTC AGTGCAAATA ATGACTTGAA CCTTAGATAT
    TATATGGGAA ACCAAGATAT CTCCTGGAAG AATCAACCAG TCCAAAATTC CAAAGGGGAG
    TGCCTCCACA GTTCTGGTTC ATCTGAAGAT GTCTGGAAAA ACTACAACCT AGACGAAATT
    GAGAGAACTT TTTGCCAAGA GCTTCCCAAG TGCTATGAAA AGGTTGAAAA AGCCCTGAGT
    GCAGGGGACC CCTCCAAAAG TGGATATGTA TCTTTGAATT ACCTAAAGAT TGTCCTCGAC
    ACCTTTGTAT ACCGATTACC AAGGAGAATT TTTATCCAGT TAATGAAAAG ATTTGGACTT
    AAAACCACCA CCAAAATCAA TTGGAAACAA TTTCTAACAT CACTTTATGA ACCCCAGTGG
    TTGGAAGTCA ACAATACAGT TCCTCCAACA AAAAGAAATA GAATCACTTC TAGAGATCAA
    TCTTGCAAGG AAAACATTAT CACAAAGTTA TCTAGACAAG AGGATCACTA TACATCATTG
    AAGAAAGCAC TCCTGATCGC CAACACTAAA CCTGATGGAC AGATAACGGG GAAAGAATTG
    CGATATATTC TAAATTGCAT GGTTGTAAAA ATCAGTGATT CAGAATTCAA AGAGCTCATG
    CAAATGCTTG ACCCTGGGGA CACCGGATGG GTTAACATCT ACACACTTAT TGAACTGCTT
    GAAGAGACCC CTAAGATACA AAAACTCTCC TCCTCTGCAG ACACCAAGGC GCCTCTCCTC
    CTGGCTTGGG ATTCAGTGGA AGAAATCATT CAGGACTCAA TTGCTAGGAA TCTCCAAGCC
    TTCTACAAAA TGCTACGGTC ATATGACCTC GGAGACACAG GGCTCATTGG TCGAAATAAT
    TTCAAGAAAA TCATGCGCAT CTTCTGTCCA TTTTTAACAA TCGAACATTT ACTGAAACTC
    TGTAGTAGGT TTCAGGATAT TGCTTCAGGA AGAATCCTTT ATAAGAAATT TTTGGCATCC
    ATGGGGATGA GTGGCCCACC ACCCCCCTCT CCGGTTATTA CTCCAAAGGA TCAGCTATTG
    AGTGACCATT TTCAAAGAGA GGAGCAGCGG AAGTTGGATC TTTCTGAAAG AACCAAGCCC
    GTGGAGGACA GAAGCACCTC GACCAAGAAA ATGACGAAGG ATGAAGCGAT CGAAAAACTA
    AAAAACTGTA TTCAGCAGCG GGACCCAGCA TTCAAAAAAT TCTTTCTTGA CTATAGCAAG
    GAGCTCACTG GCAAAATTCA TGTGCGTGAC TTCAAGAAGG TACTGGAAGC TAGTGGAATG
    CCCATGGATG ATGACCAGTA CGCTCTGCTG ACGGCAAAAA TAGGATATAA GAGAGAAGGG
    ATGAGTTATC TTGATTTTGC AGCAGGATTT GAAGATGCTA AAATGAGCAG GCCAGAAGTG
    ATCCCATTGC AGACTCCAGT TCTTTCCAAG ACTAATTTGG ACAGATACTT TATAACCGCT
    GAAGAGTGCC TGAAGCAGTT CCCCCAGAGA CTGAAGGAAG CCTTCCGGGA CCCCTACTCT
    GCCTTCTTTA AAATAGACAC TGACAGGGAT GGCATAATCA ACATGCATGA TCTTCACAGA
    TTGTTACTGC ACTTACTGTT CAATCTTAAA GACAAAGAAT TTGAGCGTTT CCTGGGCCTT
    CTTGGCTTGA GACTCAGCGT CACTCTCAAT TTTCGGGAAT TTCGAAATCT GTGTGAGAAG
    AGAGCATTGA GAACAGATGA CGCGCCTCAA AGACTAATCA GACCAAAACA GAAGGTCGCA
    GATTCAGAAC TAGCTTGTGA ACAGGCTCAT CAGTATCTTG TTACCAAAGC AAAAACCAGA
    TGGTCAGACT TATCTAAGAA TTTTATAGAA ACTGATAATG AGGGCAATGG CATCCTTCGG
    CGGCGGGATA TAAAGAATGC GCTCTATGGA TTTGATATTC CTCTGACACC GAGGGAATTT
    GAAAAGCTCT GGATGAGCTA TGACACCGAG GGAAGAGGGC ACATAACTTA CCAAGAATTT
    CTACAGAAAT TGGGGATTAA CTACTCAGCA GACATTCATC GGCCCTATGC AGAGGATTAC
    TTCAACTTCA TGGGTCATTT CACAAAACCC CAGCAGATGC AAGAGGAGGC CAGACAGCTA
    GAGCAGAGCG TGGACAGGAC TGCGCCAGTT AGGGATAAGC TTAAGGACCA TTATCAAGAT
    ATCAGCAAAG CACTCACCAA ACTAGACAAA TCCAAAAATG GCTACCTATC CATGTGCAAG
    ATGCAGAAAG TGCTGCAGGA GCTCGGCTGC TCTCTCAAGG AGGAGGAGCT GACCAATCTT
    CTGAACAGTT GGGAAATCAA ATGGCATGAT AACTCCATCA ATTACCTTGA CTTCCTGAAA
    GCAGTGGAGA GCAGCAAGCC AACAAGACCT CAGCCAAAGA AAAAAGAAGA GAGTGTGCCA
    ATCAGTTTCT CAACACTGAA CCCAGAGGAG GTTGTAAAGA ACATCCAGGA AGTTGTTGCC
    TCCTCCGACA TGGCTTTGTC AACGGCATTT TCTGCATTGG ATAAAGAGGA CACTGGGTTT
    GTAAAGGCTT CAGATTTTGG ACAAGTTCTT AAGGATTTCT GTTACAAACT AACAGACAAC
    CAGTATCATT ATTTTTTGAG AAAACTGAAA ATTCATCTAA CCCCCCATAT AAATTGGAAA
    TATTTTCTCC AGAACTTCAG TTCTTTCTCT GAAGAGACTG CTGCCGAGTG GGCTGAGAAA
    ATGCCCAAAG GCCCACCGCC CACATCTCCT CCCAAGGAAA CAGCAAACCA GGAGCTGCTG
    GCTCAGCTCC ACAAGGCGGT GGGCTCCCAC TACCATGCGA TTGCTCAGGA GTTTGACAAC
    TTCGACACCA TGAAAACGTA CACCGCCTCC AAGGATGAAT TCAGGGCCAT TTGTGCTCGC
    CATGTCCAAA TTCTGACAGA TGAGCAATTT GACAGACTGT GGAGCAAGAT GCCAGTCAAT
    GCCAAGGGGA GGCTGAAGTA CCTGGACCTC CTGAGCATGT TCAGCTCCAA GAGAGCGCCA
    ACACCATTGA CCACGGGCAG CTCGGCCAAG GCCCAGAGAG GCAGCAGTGT TCCTGAAATC
    TCAGATGGAA GCAGGCCTGC AGTATCATCG CCCATTCATG ACCTGAAGGC GGGGTTGAAA
    GCACAGAGCC ACCCCTGTAC CCCCGCTAGT GCCAGTTCGG CGCCAGGCAC CCTGCCCTTG
    CAGAACTGCG AGCCCATAGA AAGCAAGCTC CAGAAGAAAA TCCAGGGCTG CTGGCGAGAG
    TTCCTGAAAG AATGCAAAGA GAAGGATGTG AATAAGCAGG GCCAGGTCAC CACGGCTGAA
    TTCCTGGCTC TTGTGGAGAA ATTCAATCTG GACATAAGCA AAGAGGAATG TCAGCAGCTC
    ATCGTGAAGT ATGATTTAAA GAACAACGGG AAGTTTGCTT ATTGCAACTT CATCCAGAGC
    TGTGTCCTCC TGTTGAAAGC GAAGGAAACC TCACTGATGC AGAGGATGAA AATTCAGAAT
    GCACATAAAA TGAAAGAATC TGGTGCTGAG ACTTCTTCCT TTTATTCAGC TTTACTGCGT
    ATCCAGCCCA AAATTGTGCA CTGCTGGAGG CCCATGCGGC GTACCTTCAA AGCCTATGAT
    GAGGGCGGGA CAGGGCTTTT AAGCGTGGCT GATTTCAGGA AGGTCCTGAG ACAGTACAGC
    ATCAACCTCT CGGAGGAGGA GTTCTTTCAC ATTCTGGAGT ATTATGACAA GACGCTGTCT
    TCCAAGATCT CCTACAACGA CTTCCTGCGG GCGTTCCTTC AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13362
    Clone ID Related Accession (Same CDS sequence) XM_005625769.2
    Accession Version XM_005625769.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005625769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACAGA ATAGCACCAA AACACACGTT TCCACAATCA ACAGCACCAA ATCCTGCAGT 
    ACCAAGGCCA GGGATTTAAT GTGCAAAATG GCAATTATAC CAGATTGGCA AACTTCGTAT
    CCTCACACAG GAAAGCTCAA GAATTTAAGA CCTCATTCTT CACCATGTAG AGTGTATTCA
    AGGAACAGTT TCCAAAATAT GTTCAGACCT TCTTCTTCAA CCACAGCTAT TGCAAATCCG
    ATACTGTCCT TCTTAGATGT TAAACGGCTT TTATTTCAAA AAATCACTGA TAAAGGATAT
    GCGTTGAAAA AGGCTTTTGA GCTGCTTGAC ACCAGTCACA ACATGACGGT ATCAAAGAAT
    GAACTGAGAA GAATTATCAC AACCTTCCTG ATGCCTCTCA CGAGAGAACA GTTTCAGGAT
    GTACTGGCTC AGATCCCCCT CACCAGCTCT GGGGCCATTC CATACCTTGA ATTCCTGTCC
    TGGTTTGGTG GAATGGACCT AAGTATAAAT GTTATACAGA GGGGAGGTGG GAATGAAATG
    AGTTGCAGTC GGACAGTAAA AGAACTTGAA AAACAAATAG GAGAAAAGAT TTTCAGAAAC
    ATTAAAACTA TTTTCAAAGC CTTCAAGCTC ATTGATGTTA GCAAGACTGG TCTGATCCAG
    TCTTGTGAAC TGAGGAAAGT TCTCGAGACC TTCTGTTTGA AGATGAAAGA TGATGAATAC
    AAAAAGTTTG CAAAACACTA CAACATTGAT AAAGATACTG CAGTGGATTA CAATGTTTTT
    TTGAAGAACC TCAGTGCAAA TAATGACTTG AACCTTAGAT ATTATATGGG AAACCAAGAT
    ATCTCCTGGA AGAATCAACC AGTCCAAAAT TCCAAAGGGG AGTGCCTCCA CAGTTCTGGT
    TCATCTGAAG ATGTCTGGAA AAACTACAAC CTAGACGAAA TTGAGAGAAC TTTTTGCCAA
    GAGCTTCCCA AGTGCTATGA AAAGGTTGAA AAAGCCCTGA GTGCAGGGGA CCCCTCCAAA
    AGTGGATATG TATCTTTGAA TTACCTAAAG ATTGTCCTCG ACACCTTTGT ATACCGATTA
    CCAAGGAGAA TTTTTATCCA GTTAATGAAA AGATTTGGAC TTAAAACCAC CACCAAAATC
    AATTGGAAAC AATTTCTAAC ATCACTTTAT GAACCCCAGT GGTTGGAAGT CAACAATACA
    GTTCCTCCAA CAAAAAGAAA TAGAATCACT TCTAGAGATC AATCTTGCAA GGAAAACATT
    ATCACAAAGT TATCTAGACA AGAGGATCAC TATACATCAT TGAAGAAAGC ACTCCTGATC
    GCCAACACTA AACCTGATGG ACAGATAACG GGGAAAGAAT TGCGATATAT TCTAAATTGC
    ATGGTTGTAA AAATCAGTGA TTCAGAATTC AAAGAGCTCA TGCAAATGCT TGACCCTGGG
    GACACCGGAT GGGTTAACAT CTACACACTT ATTGAACTGC TTGAAGAGAC CCCTAAGATA
    CAAAAACTCT CCTCCTCTGC AGACACCAAG GCGCCTCTCC TCCTGGCTTG GGATTCAGTG
    GAAGAAATCA TTCAGGACTC AATTGCTAGG AATCTCCAAG CCTTCTACAA AATGCTACGG
    TCATATGACC TCGGAGACAC AGGGCTCATT GGTCGAAATA ATTTCAAGAA AATCATGCGC
    ATCTTCTGTC CATTTTTAAC AATCGAACAT TTACTGAAAC TCTGTAGTAG GTTTCAGGAT
    ATTGCTTCAG GAAGAATCCT TTATAAGAAA TTTTTGGCAT CCATGGGGAT GAGTGGCCCA
    CCACCCCCCT CTCCGGTTAT TACTCCAAAG GATCAGCTAT TGAGTGACCA TTTTCAAAGA
    GAGGAGCAGC GGAAGTTGGA TCTTTCTGAA AGAACCAAGC CCGTGGAGGA CAGAAGCACC
    TCGACCAAGA AAATGACGAA GGATGAAGCG ATCGAAAAAC TAAAAAACTG TATTCAGCAG
    CGGGACCCAG CATTCAAAAA ATTCTTTCTT GACTATAGCA AGGAGCTCAC TGGCAAAATT
    CATGTGCGTG ACTTCAAGAA GGTACTGGAA GCTAGTGGAA TGCCCATGGA TGATGACCAG
    TACGCTCTGC TGACGGCAAA AATAGGATAT AAGAGAGAAG GGATGAGTTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATGC TAAAATGAGC AGGCCAGAAG TGATCCCATT GCAGACTCCA
    GTTCTTTCCA AGACTAATTT GGACAGATAC TTTATAACCG CTGAAGAGTG CCTGAAGCAG
    TTCCCCCAGA GACTGAAGGA AGCCTTCCGG GACCCCTACT CTGCCTTCTT TAAAATAGAC
    ACTGACAGGG ATGGCATAAT CAACATGCAT GATCTTCACA GATTGTTACT GCACTTACTG
    TTCAATCTTA AAGACAAAGA ATTTGAGCGT TTCCTGGGCC TTCTTGGCTT GAGACTCAGC
    GTCACTCTCA ATTTTCGGGA ATTTCGAAAT CTGTGTGAGA AGAGAGCATT GAGAACAGAT
    GACGCGCCTC AAAGACTAAT CAGACCAAAA CAGAAGGTCG CAGATTCAGA ACTAGCTTGT
    GAACAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAACCA GATGGTCAGA CTTATCTAAG
    AATTTTATAG AAACTGATAA TGAGGGCAAT GGCATCCTTC GGCGGCGGGA TATAAAGAAT
    GCGCTCTATG GATTTGATAT TCCTCTGACA CCGAGGGAAT TTGAAAAGCT CTGGATGAGC
    TATGACACCG AGGGAAGAGG GCACATAACT TACCAAGAAT TTCTACAGAA ATTGGGGATT
    AACTACTCAG CAGACATTCA TCGGCCCTAT GCAGAGGATT ACTTCAACTT CATGGGTCAT
    TTCACAAAAC CCCAGCAGAT GCAAGAGGAG GCCAGACAGC TAGAGCAGAG CGTGGACAGG
    ACTGCGCCAG TTAGGGATAA GCTTAAGGAC CATTATCAAG ATATCAGCAA AGCACTCACC
    AAACTAGACA AATCCAAAAA TGGCTACCTA TCCATGTGCA AGATGCAGAA AGTGCTGCAG
    GAGCTCGGCT GCTCTCTCAA GGAGGAGGAG CTGACCAATC TTCTGAACAG TTGGGAAATC
    AAATGGCATG ATAACTCCAT CAATTACCTT GACTTCCTGA AAGCAGTGGA GAGCAGCAAG
    CCAACAAGAC CTCAGCCAAA GAAAAAAGAA GAGAGTGTGC CAATCAGTTT CTCAACACTG
    AACCCAGAGG AGGTTGTAAA GAACATCCAG GAAGTTGTTG CCTCCTCCGA CATGGCTTTG
    TCAACGGCAT TTTCTGCATT GGATAAAGAG GACACTGGGT TTGTAAAGGC TTCAGATTTT
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACAGACA ACCAGTATCA TTATTTTTTG
    AGAAAACTGA AAATTCATCT AACCCCCCAT ATAAATTGGA AATATTTTCT CCAGAACTTC
    AGTTCTTTCT CTGAAGAGAC TGCTGCCGAG TGGGCTGAGA AAATGCCCAA AGGCCCACCG
    CCCACATCTC CTCCCAAGGA AACAGCAAAC CAGGAGCTGC TGGCTCAGCT CCACAAGGCG
    GTGGGCTCCC ACTACCATGC GATTGCTCAG GAGTTTGACA ACTTCGACAC CATGAAAACG
    TACACCGCCT CCAAGGATGA ATTCAGGGCC ATTTGTGCTC GCCATGTCCA AATTCTGACA
    GATGAGCAAT TTGACAGACT GTGGAGCAAG ATGCCAGTCA ATGCCAAGGG GAGGCTGAAG
    TACCTGGACC TCCTGAGCAT GTTCAGCTCC AAGAGAGCGC CAACACCATT GACCACGGGC
    AGCTCGGCCA AGGCCCAGAG AGGCAGCAGT GTTCCTGAAA TCTCAGATGG AAGCAGGCCT
    GCAGTATCAT CGCCCATTCA TGACCTGAAG GCGGGGTTGA AAGCACAGAG CCACCCCTGT
    ACCCCCGCTA GTGCCAGTTC GGCGCCAGGC ACCCTGCCCT TGCAGAACTG CGAGCCCATA
    GAAAGCAAGC TCCAGAAGAA AATCCAGGGC TGCTGGCGAG AGTTCCTGAA AGAATGCAAA
    GAGAAGGATG TGAATAAGCA GGGCCAGGTC ACCACGGCTG AATTCCTGGC TCTTGTGGAG
    AAATTCAATC TGGACATAAG CAAAGAGGAA TGTCAGCAGC TCATCGTGAA GTATGATTTA
    AAGAACAACG GGAAGTTTGC TTATTGCAAC TTCATCCAGA GCTGTGTCCT CCTGTTGAAA
    GCGAAGGAAA CCTCACTGAT GCAGAGGATG AAAATTCAGA ATGCACATAA AATGAAAGAA
    TCTGGTGCTG AGACTTCTTC CTTTTATTCA GCTTTACTGC GTATCCAGCC CAAAATTGTG
    CACTGCTGGA GGCCCATGCG GCGTACCTTC AAAGCCTATG ATGAGGGCGG GACAGGGCTT
    TTAAGCGTGG CTGATTTCAG GAAGGTCCTG AGACAGTACA GCATCAACCT CTCGGAGGAG
    GAGTTCTTTC ACATTCTGGA GTATTATGAC AAGACGCTGT CTTCCAAGAT CTCCTACAAC
    GACTTCCTGC GGGCGTTCCT TCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005625826.1
    CDS315..4901
    Translation

    Target ORF information:

    RefSeq Version XM_005625769.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005625769.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAAACAGA ATAGCACCAA AACACACGTT TCCACAATCA ACAGCACCAA ATCCTGCAGT 
    ACCAAGGCCA GGGATTTAAT GTGCAAAATG GCAATTATAC CAGATTGGCA AACTTCGTAT
    CCTCACACAG GAAAGCTCAA GAATTTAAGA CCTCATTCTT CACCATGTAG AGTGTATTCA
    AGGAACAGTT TCCAAAATAT GTTCAGACCT TCTTCTTCAA CCACAGCTAT TGCAAATCCG
    ATACTGTCCT TCTTAGATGT TAAACGGCTT TTATTTCAAA AAATCACTGA TAAAGGATAT
    GCGTTGAAAA AGGCTTTTGA GCTGCTTGAC ACCAGTCACA ACATGACGGT ATCAAAGAAT
    GAACTGAGAA GAATTATCAC AACCTTCCTG ATGCCTCTCA CGAGAGAACA GTTTCAGGAT
    GTACTGGCTC AGATCCCCCT CACCAGCTCT GGGGCCATTC CATACCTTGA ATTCCTGTCC
    TGGTTTGGTG GAATGGACCT AAGTATAAAT GTTATACAGA GGGGAGGTGG GAATGAAATG
    AGTTGCAGTC GGACAGTAAA AGAACTTGAA AAACAAATAG GAGAAAAGAT TTTCAGAAAC
    ATTAAAACTA TTTTCAAAGC CTTCAAGCTC ATTGATGTTA GCAAGACTGG TCTGATCCAG
    TCTTGTGAAC TGAGGAAAGT TCTCGAGACC TTCTGTTTGA AGATGAAAGA TGATGAATAC
    AAAAAGTTTG CAAAACACTA CAACATTGAT AAAGATACTG CAGTGGATTA CAATGTTTTT
    TTGAAGAACC TCAGTGCAAA TAATGACTTG AACCTTAGAT ATTATATGGG AAACCAAGAT
    ATCTCCTGGA AGAATCAACC AGTCCAAAAT TCCAAAGGGG AGTGCCTCCA CAGTTCTGGT
    TCATCTGAAG ATGTCTGGAA AAACTACAAC CTAGACGAAA TTGAGAGAAC TTTTTGCCAA
    GAGCTTCCCA AGTGCTATGA AAAGGTTGAA AAAGCCCTGA GTGCAGGGGA CCCCTCCAAA
    AGTGGATATG TATCTTTGAA TTACCTAAAG ATTGTCCTCG ACACCTTTGT ATACCGATTA
    CCAAGGAGAA TTTTTATCCA GTTAATGAAA AGATTTGGAC TTAAAACCAC CACCAAAATC
    AATTGGAAAC AATTTCTAAC ATCACTTTAT GAACCCCAGT GGTTGGAAGT CAACAATACA
    GTTCCTCCAA CAAAAAGAAA TAGAATCACT TCTAGAGATC AATCTTGCAA GGAAAACATT
    ATCACAAAGT TATCTAGACA AGAGGATCAC TATACATCAT TGAAGAAAGC ACTCCTGATC
    GCCAACACTA AACCTGATGG ACAGATAACG GGGAAAGAAT TGCGATATAT TCTAAATTGC
    ATGGTTGTAA AAATCAGTGA TTCAGAATTC AAAGAGCTCA TGCAAATGCT TGACCCTGGG
    GACACCGGAT GGGTTAACAT CTACACACTT ATTGAACTGC TTGAAGAGAC CCCTAAGATA
    CAAAAACTCT CCTCCTCTGC AGACACCAAG GCGCCTCTCC TCCTGGCTTG GGATTCAGTG
    GAAGAAATCA TTCAGGACTC AATTGCTAGG AATCTCCAAG CCTTCTACAA AATGCTACGG
    TCATATGACC TCGGAGACAC AGGGCTCATT GGTCGAAATA ATTTCAAGAA AATCATGCGC
    ATCTTCTGTC CATTTTTAAC AATCGAACAT TTACTGAAAC TCTGTAGTAG GTTTCAGGAT
    ATTGCTTCAG GAAGAATCCT TTATAAGAAA TTTTTGGCAT CCATGGGGAT GAGTGGCCCA
    CCACCCCCCT CTCCGGTTAT TACTCCAAAG GATCAGCTAT TGAGTGACCA TTTTCAAAGA
    GAGGAGCAGC GGAAGTTGGA TCTTTCTGAA AGAACCAAGC CCGTGGAGGA CAGAAGCACC
    TCGACCAAGA AAATGACGAA GGATGAAGCG ATCGAAAAAC TAAAAAACTG TATTCAGCAG
    CGGGACCCAG CATTCAAAAA ATTCTTTCTT GACTATAGCA AGGAGCTCAC TGGCAAAATT
    CATGTGCGTG ACTTCAAGAA GGTACTGGAA GCTAGTGGAA TGCCCATGGA TGATGACCAG
    TACGCTCTGC TGACGGCAAA AATAGGATAT AAGAGAGAAG GGATGAGTTA TCTTGATTTT
    GCAGCAGGAT TTGAAGATGC TAAAATGAGC AGGCCAGAAG TGATCCCATT GCAGACTCCA
    GTTCTTTCCA AGACTAATTT GGACAGATAC TTTATAACCG CTGAAGAGTG CCTGAAGCAG
    TTCCCCCAGA GACTGAAGGA AGCCTTCCGG GACCCCTACT CTGCCTTCTT TAAAATAGAC
    ACTGACAGGG ATGGCATAAT CAACATGCAT GATCTTCACA GATTGTTACT GCACTTACTG
    TTCAATCTTA AAGACAAAGA ATTTGAGCGT TTCCTGGGCC TTCTTGGCTT GAGACTCAGC
    GTCACTCTCA ATTTTCGGGA ATTTCGAAAT CTGTGTGAGA AGAGAGCATT GAGAACAGAT
    GACGCGCCTC AAAGACTAAT CAGACCAAAA CAGAAGGTCG CAGATTCAGA ACTAGCTTGT
    GAACAGGCTC ATCAGTATCT TGTTACCAAA GCAAAAACCA GATGGTCAGA CTTATCTAAG
    AATTTTATAG AAACTGATAA TGAGGGCAAT GGCATCCTTC GGCGGCGGGA TATAAAGAAT
    GCGCTCTATG GATTTGATAT TCCTCTGACA CCGAGGGAAT TTGAAAAGCT CTGGATGAGC
    TATGACACCG AGGGAAGAGG GCACATAACT TACCAAGAAT TTCTACAGAA ATTGGGGATT
    AACTACTCAG CAGACATTCA TCGGCCCTAT GCAGAGGATT ACTTCAACTT CATGGGTCAT
    TTCACAAAAC CCCAGCAGAT GCAAGAGGAG GCCAGACAGC TAGAGCAGAG CGTGGACAGG
    ACTGCGCCAG TTAGGGATAA GCTTAAGGAC CATTATCAAG ATATCAGCAA AGCACTCACC
    AAACTAGACA AATCCAAAAA TGGCTACCTA TCCATGTGCA AGATGCAGAA AGTGCTGCAG
    GAGCTCGGCT GCTCTCTCAA GGAGGAGGAG CTGACCAATC TTCTGAACAG TTGGGAAATC
    AAATGGCATG ATAACTCCAT CAATTACCTT GACTTCCTGA AAGCAGTGGA GAGCAGCAAG
    CCAACAAGAC CTCAGCCAAA GAAAAAAGAA GAGAGTGTGC CAATCAGTTT CTCAACACTG
    AACCCAGAGG AGGTTGTAAA GAACATCCAG GAAGTTGTTG CCTCCTCCGA CATGGCTTTG
    TCAACGGCAT TTTCTGCATT GGATAAAGAG GACACTGGGT TTGTAAAGGC TTCAGATTTT
    GGACAAGTTC TTAAGGATTT CTGTTACAAA CTAACAGACA ACCAGTATCA TTATTTTTTG
    AGAAAACTGA AAATTCATCT AACCCCCCAT ATAAATTGGA AATATTTTCT CCAGAACTTC
    AGTTCTTTCT CTGAAGAGAC TGCTGCCGAG TGGGCTGAGA AAATGCCCAA AGGCCCACCG
    CCCACATCTC CTCCCAAGGA AACAGCAAAC CAGGAGCTGC TGGCTCAGCT CCACAAGGCG
    GTGGGCTCCC ACTACCATGC GATTGCTCAG GAGTTTGACA ACTTCGACAC CATGAAAACG
    TACACCGCCT CCAAGGATGA ATTCAGGGCC ATTTGTGCTC GCCATGTCCA AATTCTGACA
    GATGAGCAAT TTGACAGACT GTGGAGCAAG ATGCCAGTCA ATGCCAAGGG GAGGCTGAAG
    TACCTGGACC TCCTGAGCAT GTTCAGCTCC AAGAGAGCGC CAACACCATT GACCACGGGC
    AGCTCGGCCA AGGCCCAGAG AGGCAGCAGT GTTCCTGAAA TCTCAGATGG AAGCAGGCCT
    GCAGTATCAT CGCCCATTCA TGACCTGAAG GCGGGGTTGA AAGCACAGAG CCACCCCTGT
    ACCCCCGCTA GTGCCAGTTC GGCGCCAGGC ACCCTGCCCT TGCAGAACTG CGAGCCCATA
    GAAAGCAAGC TCCAGAAGAA AATCCAGGGC TGCTGGCGAG AGTTCCTGAA AGAATGCAAA
    GAGAAGGATG TGAATAAGCA GGGCCAGGTC ACCACGGCTG AATTCCTGGC TCTTGTGGAG
    AAATTCAATC TGGACATAAG CAAAGAGGAA TGTCAGCAGC TCATCGTGAA GTATGATTTA
    AAGAACAACG GGAAGTTTGC TTATTGCAAC TTCATCCAGA GCTGTGTCCT CCTGTTGAAA
    GCGAAGGAAA CCTCACTGAT GCAGAGGATG AAAATTCAGA ATGCACATAA AATGAAAGAA
    TCTGGTGCTG AGACTTCTTC CTTTTATTCA GCTTTACTGC GTATCCAGCC CAAAATTGTG
    CACTGCTGGA GGCCCATGCG GCGTACCTTC AAAGCCTATG ATGAGGGCGG GACAGGGCTT
    TTAAGCGTGG CTGATTTCAG GAAGGTCCTG AGACAGTACA GCATCAACCT CTCGGAGGAG
    GAGTTCTTTC ACATTCTGGA GTATTATGAC AAGACGCTGT CTTCCAAGAT CTCCTACAAC
    GACTTCCTGC GGGCGTTCCT TCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13363
    Clone ID Related Accession (Same CDS sequence) XM_538339.6 , XM_538339.5 , XM_022424329.1
    Accession Version XM_538339.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_538339.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATCCCC
    CTCACCAGCT CTGGGGCCAT TCCATACCTT GAATTCCTGT CCTGGTTTGG TGGAATGGAC
    CTAAGTATAA ATGTTATACA GAGGGGAGGT GGGAATGAAA TGAGTTGCAG TCGGACAGTA
    AAAGAACTTG AAAAACAAAT AGGAGAAAAG ATTTTCAGAA ACATTAAAAC TATTTTCAAA
    GCCTTCAAGC TCATTGATGT TAGCAAGACT GGTCTGATCC AGTCTTGTGA ACTGAGGAAA
    GTTCTCGAGA CCTTCTGTTT GAAGATGAAA GATGATGAAT ACAAAAAGTT TGCAAAACAC
    TACAACATTG ATAAAGATAC TGCAGTGGAT TACAATGTTT TTTTGAAGAA CCTCAGTGCA
    AATAATGACT TGAACCTTAG ATATTATATG GGAAACCAAG ATATCTCCTG GAAGAATCAA
    CCAGTCCAAA ATTCCAAAGG GGAGTGCCTC CACAGTTCTG GTTCATCTGA AGATGTCTGG
    AAAAACTACA ACCTAGACGA AATTGAGAGA ACTTTTTGCC AAGAGCTTCC CAAGTGCTAT
    GAAAAGGTTG AAAAAGCCCT GAGTGCAGGG GACCCCTCCA AAAGTGGATA TGTATCTTTG
    AATTACCTAA AGATTGTCCT CGACACCTTT GTATACCGAT TACCAAGGAG AATTTTTATC
    CAGTTAATGA AAAGATTTGG ACTTAAAACC ACCACCAAAA TCAATTGGAA ACAATTTCTA
    ACATCACTTT ATGAACCCCA GTGGTTGGAA GTCAACAATA CAGTTCCTCC AACAAAAAGA
    AATAGAATCA CTTCTAGAGA TCAATCTTGC AAGGAAAACA TTATCACAAA GTTATCTAGA
    CAAGAGGATC ACTATACATC ATTGAAGAAA GCACTCCTGA TCGCCAACAC TAAACCTGAT
    GGACAGATAA CGGGGAAAGA ATTGCGATAT ATTCTAAATT GCATGGTTGT AAAAATCAGT
    GATTCAGAAT TCAAAGAGCT CATGCAAATG CTTGACCCTG GGGACACCGG ATGGGTTAAC
    ATCTACACAC TTATTGAACT GCTTGAAGAG ACCCCTAAGA TACAAAAACT CTCCTCCTCT
    GCAGACACCA AGGCGCCTCT CCTCCTGGCT TGGGATTCAG TGGAAGAAAT CATTCAGGAC
    TCAATTGCTA GGAATCTCCA AGCCTTCTAC AAAATGCTAC GGTCATATGA CCTCGGAGAC
    ACAGGGCTCA TTGGTCGAAA TAATTTCAAG AAAATCATGC GCATCTTCTG TCCATTTTTA
    ACAATCGAAC ATTTACTGAA ACTCTGTAGT AGGTTTCAGG ATATTGCTTC AGGAAGAATC
    CTTTATAAGA AATTTTTGGC ATCCATGGGG ATGAGTGGCC CACCACCCCC CTCTCCGGTT
    ATTACTCCAA AGGATCAGCT ATTGAGTGAC CATTTTCAAA GAGAGGAGCA GCGGAAGTTG
    GATCTTTCTG AAAGAACCAA GCCCGTGGAG GACAGAAGCA CCTCGACCAA GAAAATGACG
    AAGGATGAAG CGATCGAAAA ACTAAAAAAC TGTATTCAGC AGCGGGACCC AGCATTCAAA
    AAATTCTTTC TTGACTATAG CAAGGAGCTC ACTGGCAAAA TTCATGTGCG TGACTTCAAG
    AAGGTACTGG AAGCTAGTGG AATGCCCATG GATGATGACC AGTACGCTCT GCTGACGGCA
    AAAATAGGAT ATAAGAGAGA AGGGATGAGT TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    GCTAAAATGA GCAGGCCAGA AGTGATCCCA TTGCAGACTC CAGTTCTTTC CAAGACTAAT
    TTGGACAGAT ACTTTATAAC CGCTGAAGAG TGCCTGAAGC AGTTCCCCCA GAGACTGAAG
    GAAGCCTTCC GGGACCCCTA CTCTGCCTTC TTTAAAATAG ACACTGACAG GGATGGCATA
    ATCAACATGC ATGATCTTCA CAGATTGTTA CTGCACTTAC TGTTCAATCT TAAAGACAAA
    GAATTTGAGC GTTTCCTGGG CCTTCTTGGC TTGAGACTCA GCGTCACTCT CAATTTTCGG
    GAATTTCGAA ATCTGTGTGA GAAGAGAGCA TTGAGAACAG ATGACGCGCC TCAAAGACTA
    ATCAGACCAA AACAGAAGGT CGCAGATTCA GAACTAGCTT GTGAACAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAC CAGATGGTCA GACTTATCTA AGAATTTTAT AGAAACTGAT
    AATGAGGGCA ATGGCATCCT TCGGCGGCGG GATATAAAGA ATGCGCTCTA TGGATTTGAT
    ATTCCTCTGA CACCGAGGGA ATTTGAAAAG CTCTGGATGA GCTATGACAC CGAGGGAAGA
    GGGCACATAA CTTACCAAGA ATTTCTACAG AAATTGGGGA TTAACTACTC AGCAGACATT
    CATCGGCCCT ATGCAGAGGA TTACTTCAAC TTCATGGGTC ATTTCACAAA ACCCCAGCAG
    ATGCAAGAGG AGGCCAGACA GCTAGAGCAG AGCGTGGACA GGACTGCGCC AGTTAGGGAT
    AAGCTTAAGG ACCATTATCA AGATATCAGC AAAGCACTCA CCAAACTAGA CAAATCCAAA
    AATGGCTACC TATCCATGTG CAAGATGCAG AAAGTGCTGC AGGAGCTCGG CTGCTCTCTC
    AAGGAGGAGG AGCTGACCAA TCTTCTGAAC AGTTGGGAAA TCAAATGGCA TGATAACTCC
    ATCAATTACC TTGACTTCCT GAAAGCAGTG GAGAGCAGCA AGCCAACAAG ACCTCAGCCA
    AAGAAAAAAG AAGAGAGTGT GCCAATCAGT TTCTCAACAC TGAACCCAGA GGAGGTTGTA
    AAGAACATCC AGGAAGTTGT TGCCTCCTCC GACATGGCTT TGTCAACGGC ATTTTCTGCA
    TTGGATAAAG AGGACACTGG GTTTGTAAAG GCTTCAGATT TTGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACAGA CAACCAGTAT CATTATTTTT TGAGAAAACT GAAAATTCAT
    CTAACCCCCC ATATAAATTG GAAATATTTT CTCCAGAACT TCAGTTCTTT CTCTGAAGAG
    ACTGCTGCCG AGTGGGCTGA GAAAATGCCC AAAGGCCCAC CGCCCACATC TCCTCCCAAG
    GAAACAGCAA ACCAGGAGCT GCTGGCTCAG CTCCACAAGG CGGTGGGCTC CCACTACCAT
    GCGATTGCTC AGGAGTTTGA CAACTTCGAC ACCATGAAAA CGTACACCGC CTCCAAGGAT
    GAATTCAGGG CCATTTGTGC TCGCCATGTC CAAATTCTGA CAGATGAGCA ATTTGACAGA
    CTGTGGAGCA AGATGCCAGT CAATGCCAAG GGGAGGCTGA AGTACCTGGA CCTCCTGAGC
    ATGTTCAGCT CCAAGAGAGC GCCAACACCA TTGACCACGG GCAGCTCGGC CAAGGCCCAG
    AGAGGCAGCA GTGTTCCTGA AATCTCAGAT GGAAGCAGGC CTGCAGTATC ATCGCCCATT
    CATGACCTGA AGGCGGGGTT GAAAGCACAG AGCCACCCCT GTACCCCCGC TAGTGCCAGT
    TCGGCGCCAG GCACCCTGCC CTTGCAGAAC TGCGAGCCCA TAGAAAGCAA GCTCCAGAAG
    AAAATCCAGG GCTGCTGGCG AGAGTTCCTG AAAGAATGCA AAGAGAAGGA TGTGAATAAG
    CAGGGCCAGG TCACCACGGC TGAATTCCTG GCTCTTGTGG AGAAATTCAA TCTGGACATA
    AGCAAAGAGG AATGTCAGCA GCTCATCGTG AAGTATGATT TAAAGAACAA CGGGAAGTTT
    GCTTATTGCA ACTTCATCCA GAGCTGTGTC CTCCTGTTGA AAGCGAAGGA AACCTCACTG
    ATGCAGAGGA TGAAAATTCA GAATGCACAT AAAATGAAAG AATCTGGTGC TGAGACTTCT
    TCCTTTTATT CAGCTTTACT GCGTATCCAG CCCAAAATTG TGCACTGCTG GAGGCCCATG
    CGGCGTACCT TCAAAGCCTA TGATGAGGGC GGGACAGGGC TTTTAAGCGT GGCTGATTTC
    AGGAAGGTCC TGAGACAGTA CAGCATCAAC CTCTCGGAGG AGGAGTTCTT TCACATTCTG
    GAGTATTATG ACAAGACGCT GTCTTCCAAG ATCTCCTACA ACGACTTCCT GCGGGCGTTC
    CTTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_538339.3
    CDS319..4827
    Translation

    Target ORF information:

    RefSeq Version XM_538339.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_538339.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATCCCC
    CTCACCAGCT CTGGGGCCAT TCCATACCTT GAATTCCTGT CCTGGTTTGG TGGAATGGAC
    CTAAGTATAA ATGTTATACA GAGGGGAGGT GGGAATGAAA TGAGTTGCAG TCGGACAGTA
    AAAGAACTTG AAAAACAAAT AGGAGAAAAG ATTTTCAGAA ACATTAAAAC TATTTTCAAA
    GCCTTCAAGC TCATTGATGT TAGCAAGACT GGTCTGATCC AGTCTTGTGA ACTGAGGAAA
    GTTCTCGAGA CCTTCTGTTT GAAGATGAAA GATGATGAAT ACAAAAAGTT TGCAAAACAC
    TACAACATTG ATAAAGATAC TGCAGTGGAT TACAATGTTT TTTTGAAGAA CCTCAGTGCA
    AATAATGACT TGAACCTTAG ATATTATATG GGAAACCAAG ATATCTCCTG GAAGAATCAA
    CCAGTCCAAA ATTCCAAAGG GGAGTGCCTC CACAGTTCTG GTTCATCTGA AGATGTCTGG
    AAAAACTACA ACCTAGACGA AATTGAGAGA ACTTTTTGCC AAGAGCTTCC CAAGTGCTAT
    GAAAAGGTTG AAAAAGCCCT GAGTGCAGGG GACCCCTCCA AAAGTGGATA TGTATCTTTG
    AATTACCTAA AGATTGTCCT CGACACCTTT GTATACCGAT TACCAAGGAG AATTTTTATC
    CAGTTAATGA AAAGATTTGG ACTTAAAACC ACCACCAAAA TCAATTGGAA ACAATTTCTA
    ACATCACTTT ATGAACCCCA GTGGTTGGAA GTCAACAATA CAGTTCCTCC AACAAAAAGA
    AATAGAATCA CTTCTAGAGA TCAATCTTGC AAGGAAAACA TTATCACAAA GTTATCTAGA
    CAAGAGGATC ACTATACATC ATTGAAGAAA GCACTCCTGA TCGCCAACAC TAAACCTGAT
    GGACAGATAA CGGGGAAAGA ATTGCGATAT ATTCTAAATT GCATGGTTGT AAAAATCAGT
    GATTCAGAAT TCAAAGAGCT CATGCAAATG CTTGACCCTG GGGACACCGG ATGGGTTAAC
    ATCTACACAC TTATTGAACT GCTTGAAGAG ACCCCTAAGA TACAAAAACT CTCCTCCTCT
    GCAGACACCA AGGCGCCTCT CCTCCTGGCT TGGGATTCAG TGGAAGAAAT CATTCAGGAC
    TCAATTGCTA GGAATCTCCA AGCCTTCTAC AAAATGCTAC GGTCATATGA CCTCGGAGAC
    ACAGGGCTCA TTGGTCGAAA TAATTTCAAG AAAATCATGC GCATCTTCTG TCCATTTTTA
    ACAATCGAAC ATTTACTGAA ACTCTGTAGT AGGTTTCAGG ATATTGCTTC AGGAAGAATC
    CTTTATAAGA AATTTTTGGC ATCCATGGGG ATGAGTGGCC CACCACCCCC CTCTCCGGTT
    ATTACTCCAA AGGATCAGCT ATTGAGTGAC CATTTTCAAA GAGAGGAGCA GCGGAAGTTG
    GATCTTTCTG AAAGAACCAA GCCCGTGGAG GACAGAAGCA CCTCGACCAA GAAAATGACG
    AAGGATGAAG CGATCGAAAA ACTAAAAAAC TGTATTCAGC AGCGGGACCC AGCATTCAAA
    AAATTCTTTC TTGACTATAG CAAGGAGCTC ACTGGCAAAA TTCATGTGCG TGACTTCAAG
    AAGGTACTGG AAGCTAGTGG AATGCCCATG GATGATGACC AGTACGCTCT GCTGACGGCA
    AAAATAGGAT ATAAGAGAGA AGGGATGAGT TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    GCTAAAATGA GCAGGCCAGA AGTGATCCCA TTGCAGACTC CAGTTCTTTC CAAGACTAAT
    TTGGACAGAT ACTTTATAAC CGCTGAAGAG TGCCTGAAGC AGTTCCCCCA GAGACTGAAG
    GAAGCCTTCC GGGACCCCTA CTCTGCCTTC TTTAAAATAG ACACTGACAG GGATGGCATA
    ATCAACATGC ATGATCTTCA CAGATTGTTA CTGCACTTAC TGTTCAATCT TAAAGACAAA
    GAATTTGAGC GTTTCCTGGG CCTTCTTGGC TTGAGACTCA GCGTCACTCT CAATTTTCGG
    GAATTTCGAA ATCTGTGTGA GAAGAGAGCA TTGAGAACAG ATGACGCGCC TCAAAGACTA
    ATCAGACCAA AACAGAAGGT CGCAGATTCA GAACTAGCTT GTGAACAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAC CAGATGGTCA GACTTATCTA AGAATTTTAT AGAAACTGAT
    AATGAGGGCA ATGGCATCCT TCGGCGGCGG GATATAAAGA ATGCGCTCTA TGGATTTGAT
    ATTCCTCTGA CACCGAGGGA ATTTGAAAAG CTCTGGATGA GCTATGACAC CGAGGGAAGA
    GGGCACATAA CTTACCAAGA ATTTCTACAG AAATTGGGGA TTAACTACTC AGCAGACATT
    CATCGGCCCT ATGCAGAGGA TTACTTCAAC TTCATGGGTC ATTTCACAAA ACCCCAGCAG
    ATGCAAGAGG AGGCCAGACA GCTAGAGCAG AGCGTGGACA GGACTGCGCC AGTTAGGGAT
    AAGCTTAAGG ACCATTATCA AGATATCAGC AAAGCACTCA CCAAACTAGA CAAATCCAAA
    AATGGCTACC TATCCATGTG CAAGATGCAG AAAGTGCTGC AGGAGCTCGG CTGCTCTCTC
    AAGGAGGAGG AGCTGACCAA TCTTCTGAAC AGTTGGGAAA TCAAATGGCA TGATAACTCC
    ATCAATTACC TTGACTTCCT GAAAGCAGTG GAGAGCAGCA AGCCAACAAG ACCTCAGCCA
    AAGAAAAAAG AAGAGAGTGT GCCAATCAGT TTCTCAACAC TGAACCCAGA GGAGGTTGTA
    AAGAACATCC AGGAAGTTGT TGCCTCCTCC GACATGGCTT TGTCAACGGC ATTTTCTGCA
    TTGGATAAAG AGGACACTGG GTTTGTAAAG GCTTCAGATT TTGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACAGA CAACCAGTAT CATTATTTTT TGAGAAAACT GAAAATTCAT
    CTAACCCCCC ATATAAATTG GAAATATTTT CTCCAGAACT TCAGTTCTTT CTCTGAAGAG
    ACTGCTGCCG AGTGGGCTGA GAAAATGCCC AAAGGCCCAC CGCCCACATC TCCTCCCAAG
    GAAACAGCAA ACCAGGAGCT GCTGGCTCAG CTCCACAAGG CGGTGGGCTC CCACTACCAT
    GCGATTGCTC AGGAGTTTGA CAACTTCGAC ACCATGAAAA CGTACACCGC CTCCAAGGAT
    GAATTCAGGG CCATTTGTGC TCGCCATGTC CAAATTCTGA CAGATGAGCA ATTTGACAGA
    CTGTGGAGCA AGATGCCAGT CAATGCCAAG GGGAGGCTGA AGTACCTGGA CCTCCTGAGC
    ATGTTCAGCT CCAAGAGAGC GCCAACACCA TTGACCACGG GCAGCTCGGC CAAGGCCCAG
    AGAGGCAGCA GTGTTCCTGA AATCTCAGAT GGAAGCAGGC CTGCAGTATC ATCGCCCATT
    CATGACCTGA AGGCGGGGTT GAAAGCACAG AGCCACCCCT GTACCCCCGC TAGTGCCAGT
    TCGGCGCCAG GCACCCTGCC CTTGCAGAAC TGCGAGCCCA TAGAAAGCAA GCTCCAGAAG
    AAAATCCAGG GCTGCTGGCG AGAGTTCCTG AAAGAATGCA AAGAGAAGGA TGTGAATAAG
    CAGGGCCAGG TCACCACGGC TGAATTCCTG GCTCTTGTGG AGAAATTCAA TCTGGACATA
    AGCAAAGAGG AATGTCAGCA GCTCATCGTG AAGTATGATT TAAAGAACAA CGGGAAGTTT
    GCTTATTGCA ACTTCATCCA GAGCTGTGTC CTCCTGTTGA AAGCGAAGGA AACCTCACTG
    ATGCAGAGGA TGAAAATTCA GAATGCACAT AAAATGAAAG AATCTGGTGC TGAGACTTCT
    TCCTTTTATT CAGCTTTACT GCGTATCCAG CCCAAAATTG TGCACTGCTG GAGGCCCATG
    CGGCGTACCT TCAAAGCCTA TGATGAGGGC GGGACAGGGC TTTTAAGCGT GGCTGATTTC
    AGGAAGGTCC TGAGACAGTA CAGCATCAAC CTCTCGGAGG AGGAGTTCTT TCACATTCTG
    GAGTATTATG ACAAGACGCT GTCTTCCAAG ATCTCCTACA ACGACTTCCT GCGGGCGTTC
    CTTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13363
    Clone ID Related Accession (Same CDS sequence) XM_538339.6 , XM_538339.5 , XM_022424329.1
    Accession Version XM_538339.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_538339.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATCCCC
    CTCACCAGCT CTGGGGCCAT TCCATACCTT GAATTCCTGT CCTGGTTTGG TGGAATGGAC
    CTAAGTATAA ATGTTATACA GAGGGGAGGT GGGAATGAAA TGAGTTGCAG TCGGACAGTA
    AAAGAACTTG AAAAACAAAT AGGAGAAAAG ATTTTCAGAA ACATTAAAAC TATTTTCAAA
    GCCTTCAAGC TCATTGATGT TAGCAAGACT GGTCTGATCC AGTCTTGTGA ACTGAGGAAA
    GTTCTCGAGA CCTTCTGTTT GAAGATGAAA GATGATGAAT ACAAAAAGTT TGCAAAACAC
    TACAACATTG ATAAAGATAC TGCAGTGGAT TACAATGTTT TTTTGAAGAA CCTCAGTGCA
    AATAATGACT TGAACCTTAG ATATTATATG GGAAACCAAG ATATCTCCTG GAAGAATCAA
    CCAGTCCAAA ATTCCAAAGG GGAGTGCCTC CACAGTTCTG GTTCATCTGA AGATGTCTGG
    AAAAACTACA ACCTAGACGA AATTGAGAGA ACTTTTTGCC AAGAGCTTCC CAAGTGCTAT
    GAAAAGGTTG AAAAAGCCCT GAGTGCAGGG GACCCCTCCA AAAGTGGATA TGTATCTTTG
    AATTACCTAA AGATTGTCCT CGACACCTTT GTATACCGAT TACCAAGGAG AATTTTTATC
    CAGTTAATGA AAAGATTTGG ACTTAAAACC ACCACCAAAA TCAATTGGAA ACAATTTCTA
    ACATCACTTT ATGAACCCCA GTGGTTGGAA GTCAACAATA CAGTTCCTCC AACAAAAAGA
    AATAGAATCA CTTCTAGAGA TCAATCTTGC AAGGAAAACA TTATCACAAA GTTATCTAGA
    CAAGAGGATC ACTATACATC ATTGAAGAAA GCACTCCTGA TCGCCAACAC TAAACCTGAT
    GGACAGATAA CGGGGAAAGA ATTGCGATAT ATTCTAAATT GCATGGTTGT AAAAATCAGT
    GATTCAGAAT TCAAAGAGCT CATGCAAATG CTTGACCCTG GGGACACCGG ATGGGTTAAC
    ATCTACACAC TTATTGAACT GCTTGAAGAG ACCCCTAAGA TACAAAAACT CTCCTCCTCT
    GCAGACACCA AGGCGCCTCT CCTCCTGGCT TGGGATTCAG TGGAAGAAAT CATTCAGGAC
    TCAATTGCTA GGAATCTCCA AGCCTTCTAC AAAATGCTAC GGTCATATGA CCTCGGAGAC
    ACAGGGCTCA TTGGTCGAAA TAATTTCAAG AAAATCATGC GCATCTTCTG TCCATTTTTA
    ACAATCGAAC ATTTACTGAA ACTCTGTAGT AGGTTTCAGG ATATTGCTTC AGGAAGAATC
    CTTTATAAGA AATTTTTGGC ATCCATGGGG ATGAGTGGCC CACCACCCCC CTCTCCGGTT
    ATTACTCCAA AGGATCAGCT ATTGAGTGAC CATTTTCAAA GAGAGGAGCA GCGGAAGTTG
    GATCTTTCTG AAAGAACCAA GCCCGTGGAG GACAGAAGCA CCTCGACCAA GAAAATGACG
    AAGGATGAAG CGATCGAAAA ACTAAAAAAC TGTATTCAGC AGCGGGACCC AGCATTCAAA
    AAATTCTTTC TTGACTATAG CAAGGAGCTC ACTGGCAAAA TTCATGTGCG TGACTTCAAG
    AAGGTACTGG AAGCTAGTGG AATGCCCATG GATGATGACC AGTACGCTCT GCTGACGGCA
    AAAATAGGAT ATAAGAGAGA AGGGATGAGT TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    GCTAAAATGA GCAGGCCAGA AGTGATCCCA TTGCAGACTC CAGTTCTTTC CAAGACTAAT
    TTGGACAGAT ACTTTATAAC CGCTGAAGAG TGCCTGAAGC AGTTCCCCCA GAGACTGAAG
    GAAGCCTTCC GGGACCCCTA CTCTGCCTTC TTTAAAATAG ACACTGACAG GGATGGCATA
    ATCAACATGC ATGATCTTCA CAGATTGTTA CTGCACTTAC TGTTCAATCT TAAAGACAAA
    GAATTTGAGC GTTTCCTGGG CCTTCTTGGC TTGAGACTCA GCGTCACTCT CAATTTTCGG
    GAATTTCGAA ATCTGTGTGA GAAGAGAGCA TTGAGAACAG ATGACGCGCC TCAAAGACTA
    ATCAGACCAA AACAGAAGGT CGCAGATTCA GAACTAGCTT GTGAACAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAC CAGATGGTCA GACTTATCTA AGAATTTTAT AGAAACTGAT
    AATGAGGGCA ATGGCATCCT TCGGCGGCGG GATATAAAGA ATGCGCTCTA TGGATTTGAT
    ATTCCTCTGA CACCGAGGGA ATTTGAAAAG CTCTGGATGA GCTATGACAC CGAGGGAAGA
    GGGCACATAA CTTACCAAGA ATTTCTACAG AAATTGGGGA TTAACTACTC AGCAGACATT
    CATCGGCCCT ATGCAGAGGA TTACTTCAAC TTCATGGGTC ATTTCACAAA ACCCCAGCAG
    ATGCAAGAGG AGGCCAGACA GCTAGAGCAG AGCGTGGACA GGACTGCGCC AGTTAGGGAT
    AAGCTTAAGG ACCATTATCA AGATATCAGC AAAGCACTCA CCAAACTAGA CAAATCCAAA
    AATGGCTACC TATCCATGTG CAAGATGCAG AAAGTGCTGC AGGAGCTCGG CTGCTCTCTC
    AAGGAGGAGG AGCTGACCAA TCTTCTGAAC AGTTGGGAAA TCAAATGGCA TGATAACTCC
    ATCAATTACC TTGACTTCCT GAAAGCAGTG GAGAGCAGCA AGCCAACAAG ACCTCAGCCA
    AAGAAAAAAG AAGAGAGTGT GCCAATCAGT TTCTCAACAC TGAACCCAGA GGAGGTTGTA
    AAGAACATCC AGGAAGTTGT TGCCTCCTCC GACATGGCTT TGTCAACGGC ATTTTCTGCA
    TTGGATAAAG AGGACACTGG GTTTGTAAAG GCTTCAGATT TTGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACAGA CAACCAGTAT CATTATTTTT TGAGAAAACT GAAAATTCAT
    CTAACCCCCC ATATAAATTG GAAATATTTT CTCCAGAACT TCAGTTCTTT CTCTGAAGAG
    ACTGCTGCCG AGTGGGCTGA GAAAATGCCC AAAGGCCCAC CGCCCACATC TCCTCCCAAG
    GAAACAGCAA ACCAGGAGCT GCTGGCTCAG CTCCACAAGG CGGTGGGCTC CCACTACCAT
    GCGATTGCTC AGGAGTTTGA CAACTTCGAC ACCATGAAAA CGTACACCGC CTCCAAGGAT
    GAATTCAGGG CCATTTGTGC TCGCCATGTC CAAATTCTGA CAGATGAGCA ATTTGACAGA
    CTGTGGAGCA AGATGCCAGT CAATGCCAAG GGGAGGCTGA AGTACCTGGA CCTCCTGAGC
    ATGTTCAGCT CCAAGAGAGC GCCAACACCA TTGACCACGG GCAGCTCGGC CAAGGCCCAG
    AGAGGCAGCA GTGTTCCTGA AATCTCAGAT GGAAGCAGGC CTGCAGTATC ATCGCCCATT
    CATGACCTGA AGGCGGGGTT GAAAGCACAG AGCCACCCCT GTACCCCCGC TAGTGCCAGT
    TCGGCGCCAG GCACCCTGCC CTTGCAGAAC TGCGAGCCCA TAGAAAGCAA GCTCCAGAAG
    AAAATCCAGG GCTGCTGGCG AGAGTTCCTG AAAGAATGCA AAGAGAAGGA TGTGAATAAG
    CAGGGCCAGG TCACCACGGC TGAATTCCTG GCTCTTGTGG AGAAATTCAA TCTGGACATA
    AGCAAAGAGG AATGTCAGCA GCTCATCGTG AAGTATGATT TAAAGAACAA CGGGAAGTTT
    GCTTATTGCA ACTTCATCCA GAGCTGTGTC CTCCTGTTGA AAGCGAAGGA AACCTCACTG
    ATGCAGAGGA TGAAAATTCA GAATGCACAT AAAATGAAAG AATCTGGTGC TGAGACTTCT
    TCCTTTTATT CAGCTTTACT GCGTATCCAG CCCAAAATTG TGCACTGCTG GAGGCCCATG
    CGGCGTACCT TCAAAGCCTA TGATGAGGGC GGGACAGGGC TTTTAAGCGT GGCTGATTTC
    AGGAAGGTCC TGAGACAGTA CAGCATCAAC CTCTCGGAGG AGGAGTTCTT TCACATTCTG
    GAGTATTATG ACAAGACGCT GTCTTCCAAG ATCTCCTACA ACGACTTCCT GCGGGCGTTC
    CTTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_538339.3
    CDS301..4809
    Translation

    Target ORF information:

    RefSeq Version XM_538339.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_538339.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATCCCC
    CTCACCAGCT CTGGGGCCAT TCCATACCTT GAATTCCTGT CCTGGTTTGG TGGAATGGAC
    CTAAGTATAA ATGTTATACA GAGGGGAGGT GGGAATGAAA TGAGTTGCAG TCGGACAGTA
    AAAGAACTTG AAAAACAAAT AGGAGAAAAG ATTTTCAGAA ACATTAAAAC TATTTTCAAA
    GCCTTCAAGC TCATTGATGT TAGCAAGACT GGTCTGATCC AGTCTTGTGA ACTGAGGAAA
    GTTCTCGAGA CCTTCTGTTT GAAGATGAAA GATGATGAAT ACAAAAAGTT TGCAAAACAC
    TACAACATTG ATAAAGATAC TGCAGTGGAT TACAATGTTT TTTTGAAGAA CCTCAGTGCA
    AATAATGACT TGAACCTTAG ATATTATATG GGAAACCAAG ATATCTCCTG GAAGAATCAA
    CCAGTCCAAA ATTCCAAAGG GGAGTGCCTC CACAGTTCTG GTTCATCTGA AGATGTCTGG
    AAAAACTACA ACCTAGACGA AATTGAGAGA ACTTTTTGCC AAGAGCTTCC CAAGTGCTAT
    GAAAAGGTTG AAAAAGCCCT GAGTGCAGGG GACCCCTCCA AAAGTGGATA TGTATCTTTG
    AATTACCTAA AGATTGTCCT CGACACCTTT GTATACCGAT TACCAAGGAG AATTTTTATC
    CAGTTAATGA AAAGATTTGG ACTTAAAACC ACCACCAAAA TCAATTGGAA ACAATTTCTA
    ACATCACTTT ATGAACCCCA GTGGTTGGAA GTCAACAATA CAGTTCCTCC AACAAAAAGA
    AATAGAATCA CTTCTAGAGA TCAATCTTGC AAGGAAAACA TTATCACAAA GTTATCTAGA
    CAAGAGGATC ACTATACATC ATTGAAGAAA GCACTCCTGA TCGCCAACAC TAAACCTGAT
    GGACAGATAA CGGGGAAAGA ATTGCGATAT ATTCTAAATT GCATGGTTGT AAAAATCAGT
    GATTCAGAAT TCAAAGAGCT CATGCAAATG CTTGACCCTG GGGACACCGG ATGGGTTAAC
    ATCTACACAC TTATTGAACT GCTTGAAGAG ACCCCTAAGA TACAAAAACT CTCCTCCTCT
    GCAGACACCA AGGCGCCTCT CCTCCTGGCT TGGGATTCAG TGGAAGAAAT CATTCAGGAC
    TCAATTGCTA GGAATCTCCA AGCCTTCTAC AAAATGCTAC GGTCATATGA CCTCGGAGAC
    ACAGGGCTCA TTGGTCGAAA TAATTTCAAG AAAATCATGC GCATCTTCTG TCCATTTTTA
    ACAATCGAAC ATTTACTGAA ACTCTGTAGT AGGTTTCAGG ATATTGCTTC AGGAAGAATC
    CTTTATAAGA AATTTTTGGC ATCCATGGGG ATGAGTGGCC CACCACCCCC CTCTCCGGTT
    ATTACTCCAA AGGATCAGCT ATTGAGTGAC CATTTTCAAA GAGAGGAGCA GCGGAAGTTG
    GATCTTTCTG AAAGAACCAA GCCCGTGGAG GACAGAAGCA CCTCGACCAA GAAAATGACG
    AAGGATGAAG CGATCGAAAA ACTAAAAAAC TGTATTCAGC AGCGGGACCC AGCATTCAAA
    AAATTCTTTC TTGACTATAG CAAGGAGCTC ACTGGCAAAA TTCATGTGCG TGACTTCAAG
    AAGGTACTGG AAGCTAGTGG AATGCCCATG GATGATGACC AGTACGCTCT GCTGACGGCA
    AAAATAGGAT ATAAGAGAGA AGGGATGAGT TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    GCTAAAATGA GCAGGCCAGA AGTGATCCCA TTGCAGACTC CAGTTCTTTC CAAGACTAAT
    TTGGACAGAT ACTTTATAAC CGCTGAAGAG TGCCTGAAGC AGTTCCCCCA GAGACTGAAG
    GAAGCCTTCC GGGACCCCTA CTCTGCCTTC TTTAAAATAG ACACTGACAG GGATGGCATA
    ATCAACATGC ATGATCTTCA CAGATTGTTA CTGCACTTAC TGTTCAATCT TAAAGACAAA
    GAATTTGAGC GTTTCCTGGG CCTTCTTGGC TTGAGACTCA GCGTCACTCT CAATTTTCGG
    GAATTTCGAA ATCTGTGTGA GAAGAGAGCA TTGAGAACAG ATGACGCGCC TCAAAGACTA
    ATCAGACCAA AACAGAAGGT CGCAGATTCA GAACTAGCTT GTGAACAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAC CAGATGGTCA GACTTATCTA AGAATTTTAT AGAAACTGAT
    AATGAGGGCA ATGGCATCCT TCGGCGGCGG GATATAAAGA ATGCGCTCTA TGGATTTGAT
    ATTCCTCTGA CACCGAGGGA ATTTGAAAAG CTCTGGATGA GCTATGACAC CGAGGGAAGA
    GGGCACATAA CTTACCAAGA ATTTCTACAG AAATTGGGGA TTAACTACTC AGCAGACATT
    CATCGGCCCT ATGCAGAGGA TTACTTCAAC TTCATGGGTC ATTTCACAAA ACCCCAGCAG
    ATGCAAGAGG AGGCCAGACA GCTAGAGCAG AGCGTGGACA GGACTGCGCC AGTTAGGGAT
    AAGCTTAAGG ACCATTATCA AGATATCAGC AAAGCACTCA CCAAACTAGA CAAATCCAAA
    AATGGCTACC TATCCATGTG CAAGATGCAG AAAGTGCTGC AGGAGCTCGG CTGCTCTCTC
    AAGGAGGAGG AGCTGACCAA TCTTCTGAAC AGTTGGGAAA TCAAATGGCA TGATAACTCC
    ATCAATTACC TTGACTTCCT GAAAGCAGTG GAGAGCAGCA AGCCAACAAG ACCTCAGCCA
    AAGAAAAAAG AAGAGAGTGT GCCAATCAGT TTCTCAACAC TGAACCCAGA GGAGGTTGTA
    AAGAACATCC AGGAAGTTGT TGCCTCCTCC GACATGGCTT TGTCAACGGC ATTTTCTGCA
    TTGGATAAAG AGGACACTGG GTTTGTAAAG GCTTCAGATT TTGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACAGA CAACCAGTAT CATTATTTTT TGAGAAAACT GAAAATTCAT
    CTAACCCCCC ATATAAATTG GAAATATTTT CTCCAGAACT TCAGTTCTTT CTCTGAAGAG
    ACTGCTGCCG AGTGGGCTGA GAAAATGCCC AAAGGCCCAC CGCCCACATC TCCTCCCAAG
    GAAACAGCAA ACCAGGAGCT GCTGGCTCAG CTCCACAAGG CGGTGGGCTC CCACTACCAT
    GCGATTGCTC AGGAGTTTGA CAACTTCGAC ACCATGAAAA CGTACACCGC CTCCAAGGAT
    GAATTCAGGG CCATTTGTGC TCGCCATGTC CAAATTCTGA CAGATGAGCA ATTTGACAGA
    CTGTGGAGCA AGATGCCAGT CAATGCCAAG GGGAGGCTGA AGTACCTGGA CCTCCTGAGC
    ATGTTCAGCT CCAAGAGAGC GCCAACACCA TTGACCACGG GCAGCTCGGC CAAGGCCCAG
    AGAGGCAGCA GTGTTCCTGA AATCTCAGAT GGAAGCAGGC CTGCAGTATC ATCGCCCATT
    CATGACCTGA AGGCGGGGTT GAAAGCACAG AGCCACCCCT GTACCCCCGC TAGTGCCAGT
    TCGGCGCCAG GCACCCTGCC CTTGCAGAAC TGCGAGCCCA TAGAAAGCAA GCTCCAGAAG
    AAAATCCAGG GCTGCTGGCG AGAGTTCCTG AAAGAATGCA AAGAGAAGGA TGTGAATAAG
    CAGGGCCAGG TCACCACGGC TGAATTCCTG GCTCTTGTGG AGAAATTCAA TCTGGACATA
    AGCAAAGAGG AATGTCAGCA GCTCATCGTG AAGTATGATT TAAAGAACAA CGGGAAGTTT
    GCTTATTGCA ACTTCATCCA GAGCTGTGTC CTCCTGTTGA AAGCGAAGGA AACCTCACTG
    ATGCAGAGGA TGAAAATTCA GAATGCACAT AAAATGAAAG AATCTGGTGC TGAGACTTCT
    TCCTTTTATT CAGCTTTACT GCGTATCCAG CCCAAAATTG TGCACTGCTG GAGGCCCATG
    CGGCGTACCT TCAAAGCCTA TGATGAGGGC GGGACAGGGC TTTTAAGCGT GGCTGATTTC
    AGGAAGGTCC TGAGACAGTA CAGCATCAAC CTCTCGGAGG AGGAGTTCTT TCACATTCTG
    GAGTATTATG ACAAGACGCT GTCTTCCAAG ATCTCCTACA ACGACTTCCT GCGGGCGTTC
    CTTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13363
    Clone ID Related Accession (Same CDS sequence) XM_538339.6 , XM_538339.5 , XM_022424329.1
    Accession Version XM_022424329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product EF-hand calcium-binding domain-containing protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATCCCC
    CTCACCAGCT CTGGGGCCAT TCCATACCTT GAATTCCTGT CCTGGTTTGG TGGAATGGAC
    CTAAGTATAA ATGTTATACA GAGGGGAGGT GGGAATGAAA TGAGTTGCAG TCGGACAGTA
    AAAGAACTTG AAAAACAAAT AGGAGAAAAG ATTTTCAGAA ACATTAAAAC TATTTTCAAA
    GCCTTCAAGC TCATTGATGT TAGCAAGACT GGTCTGATCC AGTCTTGTGA ACTGAGGAAA
    GTTCTCGAGA CCTTCTGTTT GAAGATGAAA GATGATGAAT ACAAAAAGTT TGCAAAACAC
    TACAACATTG ATAAAGATAC TGCAGTGGAT TACAATGTTT TTTTGAAGAA CCTCAGTGCA
    AATAATGACT TGAACCTTAG ATATTATATG GGAAACCAAG ATATCTCCTG GAAGAATCAA
    CCAGTCCAAA ATTCCAAAGG GGAGTGCCTC CACAGTTCTG GTTCATCTGA AGATGTCTGG
    AAAAACTACA ACCTAGACGA AATTGAGAGA ACTTTTTGCC AAGAGCTTCC CAAGTGCTAT
    GAAAAGGTTG AAAAAGCCCT GAGTGCAGGG GACCCCTCCA AAAGTGGATA TGTATCTTTG
    AATTACCTAA AGATTGTCCT CGACACCTTT GTATACCGAT TACCAAGGAG AATTTTTATC
    CAGTTAATGA AAAGATTTGG ACTTAAAACC ACCACCAAAA TCAATTGGAA ACAATTTCTA
    ACATCACTTT ATGAACCCCA GTGGTTGGAA GTCAACAATA CAGTTCCTCC AACAAAAAGA
    AATAGAATCA CTTCTAGAGA TCAATCTTGC AAGGAAAACA TTATCACAAA GTTATCTAGA
    CAAGAGGATC ACTATACATC ATTGAAGAAA GCACTCCTGA TCGCCAACAC TAAACCTGAT
    GGACAGATAA CGGGGAAAGA ATTGCGATAT ATTCTAAATT GCATGGTTGT AAAAATCAGT
    GATTCAGAAT TCAAAGAGCT CATGCAAATG CTTGACCCTG GGGACACCGG ATGGGTTAAC
    ATCTACACAC TTATTGAACT GCTTGAAGAG ACCCCTAAGA TACAAAAACT CTCCTCCTCT
    GCAGACACCA AGGCGCCTCT CCTCCTGGCT TGGGATTCAG TGGAAGAAAT CATTCAGGAC
    TCAATTGCTA GGAATCTCCA AGCCTTCTAC AAAATGCTAC GGTCATATGA CCTCGGAGAC
    ACAGGGCTCA TTGGTCGAAA TAATTTCAAG AAAATCATGC GCATCTTCTG TCCATTTTTA
    ACAATCGAAC ATTTACTGAA ACTCTGTAGT AGGTTTCAGG ATATTGCTTC AGGAAGAATC
    CTTTATAAGA AATTTTTGGC ATCCATGGGG ATGAGTGGCC CACCACCCCC CTCTCCGGTT
    ATTACTCCAA AGGATCAGCT ATTGAGTGAC CATTTTCAAA GAGAGGAGCA GCGGAAGTTG
    GATCTTTCTG AAAGAACCAA GCCCGTGGAG GACAGAAGCA CCTCGACCAA GAAAATGACG
    AAGGATGAAG CGATCGAAAA ACTAAAAAAC TGTATTCAGC AGCGGGACCC AGCATTCAAA
    AAATTCTTTC TTGACTATAG CAAGGAGCTC ACTGGCAAAA TTCATGTGCG TGACTTCAAG
    AAGGTACTGG AAGCTAGTGG AATGCCCATG GATGATGACC AGTACGCTCT GCTGACGGCA
    AAAATAGGAT ATAAGAGAGA AGGGATGAGT TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    GCTAAAATGA GCAGGCCAGA AGTGATCCCA TTGCAGACTC CAGTTCTTTC CAAGACTAAT
    TTGGACAGAT ACTTTATAAC CGCTGAAGAG TGCCTGAAGC AGTTCCCCCA GAGACTGAAG
    GAAGCCTTCC GGGACCCCTA CTCTGCCTTC TTTAAAATAG ACACTGACAG GGATGGCATA
    ATCAACATGC ATGATCTTCA CAGATTGTTA CTGCACTTAC TGTTCAATCT TAAAGACAAA
    GAATTTGAGC GTTTCCTGGG CCTTCTTGGC TTGAGACTCA GCGTCACTCT CAATTTTCGG
    GAATTTCGAA ATCTGTGTGA GAAGAGAGCA TTGAGAACAG ATGACGCGCC TCAAAGACTA
    ATCAGACCAA AACAGAAGGT CGCAGATTCA GAACTAGCTT GTGAACAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAC CAGATGGTCA GACTTATCTA AGAATTTTAT AGAAACTGAT
    AATGAGGGCA ATGGCATCCT TCGGCGGCGG GATATAAAGA ATGCGCTCTA TGGATTTGAT
    ATTCCTCTGA CACCGAGGGA ATTTGAAAAG CTCTGGATGA GCTATGACAC CGAGGGAAGA
    GGGCACATAA CTTACCAAGA ATTTCTACAG AAATTGGGGA TTAACTACTC AGCAGACATT
    CATCGGCCCT ATGCAGAGGA TTACTTCAAC TTCATGGGTC ATTTCACAAA ACCCCAGCAG
    ATGCAAGAGG AGGCCAGACA GCTAGAGCAG AGCGTGGACA GGACTGCGCC AGTTAGGGAT
    AAGCTTAAGG ACCATTATCA AGATATCAGC AAAGCACTCA CCAAACTAGA CAAATCCAAA
    AATGGCTACC TATCCATGTG CAAGATGCAG AAAGTGCTGC AGGAGCTCGG CTGCTCTCTC
    AAGGAGGAGG AGCTGACCAA TCTTCTGAAC AGTTGGGAAA TCAAATGGCA TGATAACTCC
    ATCAATTACC TTGACTTCCT GAAAGCAGTG GAGAGCAGCA AGCCAACAAG ACCTCAGCCA
    AAGAAAAAAG AAGAGAGTGT GCCAATCAGT TTCTCAACAC TGAACCCAGA GGAGGTTGTA
    AAGAACATCC AGGAAGTTGT TGCCTCCTCC GACATGGCTT TGTCAACGGC ATTTTCTGCA
    TTGGATAAAG AGGACACTGG GTTTGTAAAG GCTTCAGATT TTGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACAGA CAACCAGTAT CATTATTTTT TGAGAAAACT GAAAATTCAT
    CTAACCCCCC ATATAAATTG GAAATATTTT CTCCAGAACT TCAGTTCTTT CTCTGAAGAG
    ACTGCTGCCG AGTGGGCTGA GAAAATGCCC AAAGGCCCAC CGCCCACATC TCCTCCCAAG
    GAAACAGCAA ACCAGGAGCT GCTGGCTCAG CTCCACAAGG CGGTGGGCTC CCACTACCAT
    GCGATTGCTC AGGAGTTTGA CAACTTCGAC ACCATGAAAA CGTACACCGC CTCCAAGGAT
    GAATTCAGGG CCATTTGTGC TCGCCATGTC CAAATTCTGA CAGATGAGCA ATTTGACAGA
    CTGTGGAGCA AGATGCCAGT CAATGCCAAG GGGAGGCTGA AGTACCTGGA CCTCCTGAGC
    ATGTTCAGCT CCAAGAGAGC GCCAACACCA TTGACCACGG GCAGCTCGGC CAAGGCCCAG
    AGAGGCAGCA GTGTTCCTGA AATCTCAGAT GGAAGCAGGC CTGCAGTATC ATCGCCCATT
    CATGACCTGA AGGCGGGGTT GAAAGCACAG AGCCACCCCT GTACCCCCGC TAGTGCCAGT
    TCGGCGCCAG GCACCCTGCC CTTGCAGAAC TGCGAGCCCA TAGAAAGCAA GCTCCAGAAG
    AAAATCCAGG GCTGCTGGCG AGAGTTCCTG AAAGAATGCA AAGAGAAGGA TGTGAATAAG
    CAGGGCCAGG TCACCACGGC TGAATTCCTG GCTCTTGTGG AGAAATTCAA TCTGGACATA
    AGCAAAGAGG AATGTCAGCA GCTCATCGTG AAGTATGATT TAAAGAACAA CGGGAAGTTT
    GCTTATTGCA ACTTCATCCA GAGCTGTGTC CTCCTGTTGA AAGCGAAGGA AACCTCACTG
    ATGCAGAGGA TGAAAATTCA GAATGCACAT AAAATGAAAG AATCTGGTGC TGAGACTTCT
    TCCTTTTATT CAGCTTTACT GCGTATCCAG CCCAAAATTG TGCACTGCTG GAGGCCCATG
    CGGCGTACCT TCAAAGCCTA TGATGAGGGC GGGACAGGGC TTTTAAGCGT GGCTGATTTC
    AGGAAGGTCC TGAGACAGTA CAGCATCAAC CTCTCGGAGG AGGAGTTCTT TCACATTCTG
    GAGTATTATG ACAAGACGCT GTCTTCCAAG ATCTCCTACA ACGACTTCCT GCGGGCGTTC
    CTTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280037.1
    CDS33..4541
    Translation

    Target ORF information:

    RefSeq Version XM_022424329.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris EF-hand calcium binding domain 6 (EFCAB6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTGCAAAA TGGCAATTAT ACCAGATTGG CAAACTTCGT ATCCTCACAC AGGAAAGCTC 
    AAGAATTTAA GACCTCATTC TTCACCATGT AGAGTGTATT CAAGGAACAG TTTCCAAAAT
    ATGTTCAGAC CTTCTTCTTC AACCACAGCT ATTGCAAATC CGATACTGTC CTTCTTAGAT
    GTTAAACGGC TTTTATTTCA AAAAATCACT GATAAAGGAT ATGCGTTGAA AAAGGCTTTT
    GAGCTGCTTG ACACCAGTCA CAACATGACG GTATCAAAGA ATGAACTGAG AAGAATTATC
    ACAACCTTCC TGATGCCTCT CACGAGAGAA CAGTTTCAGG ATGTACTGGC TCAGATCCCC
    CTCACCAGCT CTGGGGCCAT TCCATACCTT GAATTCCTGT CCTGGTTTGG TGGAATGGAC
    CTAAGTATAA ATGTTATACA GAGGGGAGGT GGGAATGAAA TGAGTTGCAG TCGGACAGTA
    AAAGAACTTG AAAAACAAAT AGGAGAAAAG ATTTTCAGAA ACATTAAAAC TATTTTCAAA
    GCCTTCAAGC TCATTGATGT TAGCAAGACT GGTCTGATCC AGTCTTGTGA ACTGAGGAAA
    GTTCTCGAGA CCTTCTGTTT GAAGATGAAA GATGATGAAT ACAAAAAGTT TGCAAAACAC
    TACAACATTG ATAAAGATAC TGCAGTGGAT TACAATGTTT TTTTGAAGAA CCTCAGTGCA
    AATAATGACT TGAACCTTAG ATATTATATG GGAAACCAAG ATATCTCCTG GAAGAATCAA
    CCAGTCCAAA ATTCCAAAGG GGAGTGCCTC CACAGTTCTG GTTCATCTGA AGATGTCTGG
    AAAAACTACA ACCTAGACGA AATTGAGAGA ACTTTTTGCC AAGAGCTTCC CAAGTGCTAT
    GAAAAGGTTG AAAAAGCCCT GAGTGCAGGG GACCCCTCCA AAAGTGGATA TGTATCTTTG
    AATTACCTAA AGATTGTCCT CGACACCTTT GTATACCGAT TACCAAGGAG AATTTTTATC
    CAGTTAATGA AAAGATTTGG ACTTAAAACC ACCACCAAAA TCAATTGGAA ACAATTTCTA
    ACATCACTTT ATGAACCCCA GTGGTTGGAA GTCAACAATA CAGTTCCTCC AACAAAAAGA
    AATAGAATCA CTTCTAGAGA TCAATCTTGC AAGGAAAACA TTATCACAAA GTTATCTAGA
    CAAGAGGATC ACTATACATC ATTGAAGAAA GCACTCCTGA TCGCCAACAC TAAACCTGAT
    GGACAGATAA CGGGGAAAGA ATTGCGATAT ATTCTAAATT GCATGGTTGT AAAAATCAGT
    GATTCAGAAT TCAAAGAGCT CATGCAAATG CTTGACCCTG GGGACACCGG ATGGGTTAAC
    ATCTACACAC TTATTGAACT GCTTGAAGAG ACCCCTAAGA TACAAAAACT CTCCTCCTCT
    GCAGACACCA AGGCGCCTCT CCTCCTGGCT TGGGATTCAG TGGAAGAAAT CATTCAGGAC
    TCAATTGCTA GGAATCTCCA AGCCTTCTAC AAAATGCTAC GGTCATATGA CCTCGGAGAC
    ACAGGGCTCA TTGGTCGAAA TAATTTCAAG AAAATCATGC GCATCTTCTG TCCATTTTTA
    ACAATCGAAC ATTTACTGAA ACTCTGTAGT AGGTTTCAGG ATATTGCTTC AGGAAGAATC
    CTTTATAAGA AATTTTTGGC ATCCATGGGG ATGAGTGGCC CACCACCCCC CTCTCCGGTT
    ATTACTCCAA AGGATCAGCT ATTGAGTGAC CATTTTCAAA GAGAGGAGCA GCGGAAGTTG
    GATCTTTCTG AAAGAACCAA GCCCGTGGAG GACAGAAGCA CCTCGACCAA GAAAATGACG
    AAGGATGAAG CGATCGAAAA ACTAAAAAAC TGTATTCAGC AGCGGGACCC AGCATTCAAA
    AAATTCTTTC TTGACTATAG CAAGGAGCTC ACTGGCAAAA TTCATGTGCG TGACTTCAAG
    AAGGTACTGG AAGCTAGTGG AATGCCCATG GATGATGACC AGTACGCTCT GCTGACGGCA
    AAAATAGGAT ATAAGAGAGA AGGGATGAGT TATCTTGATT TTGCAGCAGG ATTTGAAGAT
    GCTAAAATGA GCAGGCCAGA AGTGATCCCA TTGCAGACTC CAGTTCTTTC CAAGACTAAT
    TTGGACAGAT ACTTTATAAC CGCTGAAGAG TGCCTGAAGC AGTTCCCCCA GAGACTGAAG
    GAAGCCTTCC GGGACCCCTA CTCTGCCTTC TTTAAAATAG ACACTGACAG GGATGGCATA
    ATCAACATGC ATGATCTTCA CAGATTGTTA CTGCACTTAC TGTTCAATCT TAAAGACAAA
    GAATTTGAGC GTTTCCTGGG CCTTCTTGGC TTGAGACTCA GCGTCACTCT CAATTTTCGG
    GAATTTCGAA ATCTGTGTGA GAAGAGAGCA TTGAGAACAG ATGACGCGCC TCAAAGACTA
    ATCAGACCAA AACAGAAGGT CGCAGATTCA GAACTAGCTT GTGAACAGGC TCATCAGTAT
    CTTGTTACCA AAGCAAAAAC CAGATGGTCA GACTTATCTA AGAATTTTAT AGAAACTGAT
    AATGAGGGCA ATGGCATCCT TCGGCGGCGG GATATAAAGA ATGCGCTCTA TGGATTTGAT
    ATTCCTCTGA CACCGAGGGA ATTTGAAAAG CTCTGGATGA GCTATGACAC CGAGGGAAGA
    GGGCACATAA CTTACCAAGA ATTTCTACAG AAATTGGGGA TTAACTACTC AGCAGACATT
    CATCGGCCCT ATGCAGAGGA TTACTTCAAC TTCATGGGTC ATTTCACAAA ACCCCAGCAG
    ATGCAAGAGG AGGCCAGACA GCTAGAGCAG AGCGTGGACA GGACTGCGCC AGTTAGGGAT
    AAGCTTAAGG ACCATTATCA AGATATCAGC AAAGCACTCA CCAAACTAGA CAAATCCAAA
    AATGGCTACC TATCCATGTG CAAGATGCAG AAAGTGCTGC AGGAGCTCGG CTGCTCTCTC
    AAGGAGGAGG AGCTGACCAA TCTTCTGAAC AGTTGGGAAA TCAAATGGCA TGATAACTCC
    ATCAATTACC TTGACTTCCT GAAAGCAGTG GAGAGCAGCA AGCCAACAAG ACCTCAGCCA
    AAGAAAAAAG AAGAGAGTGT GCCAATCAGT TTCTCAACAC TGAACCCAGA GGAGGTTGTA
    AAGAACATCC AGGAAGTTGT TGCCTCCTCC GACATGGCTT TGTCAACGGC ATTTTCTGCA
    TTGGATAAAG AGGACACTGG GTTTGTAAAG GCTTCAGATT TTGGACAAGT TCTTAAGGAT
    TTCTGTTACA AACTAACAGA CAACCAGTAT CATTATTTTT TGAGAAAACT GAAAATTCAT
    CTAACCCCCC ATATAAATTG GAAATATTTT CTCCAGAACT TCAGTTCTTT CTCTGAAGAG
    ACTGCTGCCG AGTGGGCTGA GAAAATGCCC AAAGGCCCAC CGCCCACATC TCCTCCCAAG
    GAAACAGCAA ACCAGGAGCT GCTGGCTCAG CTCCACAAGG CGGTGGGCTC CCACTACCAT
    GCGATTGCTC AGGAGTTTGA CAACTTCGAC ACCATGAAAA CGTACACCGC CTCCAAGGAT
    GAATTCAGGG CCATTTGTGC TCGCCATGTC CAAATTCTGA CAGATGAGCA ATTTGACAGA
    CTGTGGAGCA AGATGCCAGT CAATGCCAAG GGGAGGCTGA AGTACCTGGA CCTCCTGAGC
    ATGTTCAGCT CCAAGAGAGC GCCAACACCA TTGACCACGG GCAGCTCGGC CAAGGCCCAG
    AGAGGCAGCA GTGTTCCTGA AATCTCAGAT GGAAGCAGGC CTGCAGTATC ATCGCCCATT
    CATGACCTGA AGGCGGGGTT GAAAGCACAG AGCCACCCCT GTACCCCCGC TAGTGCCAGT
    TCGGCGCCAG GCACCCTGCC CTTGCAGAAC TGCGAGCCCA TAGAAAGCAA GCTCCAGAAG
    AAAATCCAGG GCTGCTGGCG AGAGTTCCTG AAAGAATGCA AAGAGAAGGA TGTGAATAAG
    CAGGGCCAGG TCACCACGGC TGAATTCCTG GCTCTTGTGG AGAAATTCAA TCTGGACATA
    AGCAAAGAGG AATGTCAGCA GCTCATCGTG AAGTATGATT TAAAGAACAA CGGGAAGTTT
    GCTTATTGCA ACTTCATCCA GAGCTGTGTC CTCCTGTTGA AAGCGAAGGA AACCTCACTG
    ATGCAGAGGA TGAAAATTCA GAATGCACAT AAAATGAAAG AATCTGGTGC TGAGACTTCT
    TCCTTTTATT CAGCTTTACT GCGTATCCAG CCCAAAATTG TGCACTGCTG GAGGCCCATG
    CGGCGTACCT TCAAAGCCTA TGATGAGGGC GGGACAGGGC TTTTAAGCGT GGCTGATTTC
    AGGAAGGTCC TGAGACAGTA CAGCATCAAC CTCTCGGAGG AGGAGTTCTT TCACATTCTG
    GAGTATTATG ACAAGACGCT GTCTTCCAAG ATCTCCTACA ACGACTTCCT GCGGGCGTTC
    CTTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.