HHIP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HHIP
Entrez Gene ID	64399
Full Name	hedgehog interacting protein
Synonyms	HIP
General protein information	Preferred Names hedgehog interacting protein Names hedgehog-interacting protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q31.21
Summary	This gene encodes a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.[provided by RefSeq, Feb 2011].
Disorder MIM:	606178

mRNA and Protein(s)

mRNA	Protein	Name
XM_005263178.4	XP_005263235.1	hedgehog-interacting protein isoform X1
NM_022475.2	NP_071920.1	hedgehog-interacting protein precursor
NM_022475.3	NP_071920.1	hedgehog-interacting protein precursor
XM_005263178.5	XP_005263235.1	hedgehog-interacting protein isoform X1
XM_006714288.4	XP_006714351.1	hedgehog-interacting protein isoform X2
XM_006714288.3	XP_006714351.1	hedgehog-interacting protein isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	HHIP	XP_420424.4
Danio rerio (zebrafish)	hhip	NP_001073481.1
Homo sapiens (human)	HHIP	NP_071920.1
	HHIP	XP_001093950.1
Rattus norvegicus (Norway rat)	Hhip	NP_001178746.1
Pan troglodytes (chimpanzee)	HHIP	XP_001146476.1
Canis lupus familiaris (dog)	HHIP	XP_003639584.1
Mus musculus (house mouse)	Hhip	NP_064655.4
Xenopus tropicalis (tropical clawed frog)	hhip	NP_001007191.1
Bos taurus (cattle)	HHIP	NP_001092584.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of the role of HHIP SNPs in susceptibility to chronic obstructive pulmonary disease between Chinese Han and Mongolian populations.
Xu G, Gao X, Zhang S, Wang Y, Ding M, Liu W, Shen J, Sun D
Gene63750-56(2017 Dec)

Hedgehog-Interacting Protein (HIP) Regulates Apoptosis Evasion and Angiogenic Function of Late Endothelial Progenitor Cells.
Lee BNR, Son YS, Lee D, Choi YJ, Kwon SM, Chang HK, Kim PH, Cho JY
Scientific reports7(1)12449(2017 Sep)

Genetic Variants in the Hedgehog Interacting Protein Gene Are Associated with the FEV1/FVC Ratio in Southern Han Chinese Subjects with Chronic Obstructive Pulmonary Disease.
Zhang Z, Wang J, Zheng Z, Chen X, Zeng X, Zhang Y, Li D, Shu J, Yang K, Lai N, Dong L, Lu W
BioMed research international20172756726(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Single nucleotide polymorphism in HHIP gene is associated with chronic obstructive pulmonary disease.
Title: Comparison of the role of HHIP SNPs in susceptibility to chronic obstructive pulmonary disease between Chinese Han and Mongolian populations.

loss of AT2 R is associated with podocyte loss/dysfunction and is mediated, at least in part, via augmented ectopic hedgehog interacting protein expression in podocytes
Title: AT

Results showed that smoking and HHIP variant rs7654947 were associated with chronic obstructive pulmonary disease (COPD)development and lung function decline. Moreover, we found that cigarette smoking and gene susceptibility have cooperative effects on COPD risk and lung function decline
Title: Smoking status and gene susceptibility play important roles in the development of chronic obstructive pulmonary disease and lung function decline: A population-based prospective study.

this study shows that HHIP confers a risk for airway obstruction in general that is not driven exclusively by cigarette smoking, which is the main risk factor for chronic obstructive pulmonary disease
Title: Genome-wide association study on the FEV

HHIP plays a role in lung branching development, and reduced levels of HHIP ultimately result in lung hypoplasia.
Title: The Well-Known Gene HHIP and Novel Gene MECR Are Implicated in Small Airway Obstruction.

The following HHIP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HHIP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27070	NM_022475.3 Latest version!	Homo sapiens hedgehog interacting protein (HHIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu39052	XM_005263178.5 Latest version!	Homo sapiens hedgehog interacting protein (HHIP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu39053	XM_006714288.4 Latest version!	Homo sapiens hedgehog interacting protein (HHIP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu27070	NM_022475.2	Homo sapiens hedgehog interacting protein (HHIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu39052	XM_005263178.4	Homo sapiens hedgehog interacting protein (HHIP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu39053	XM_006714288.3	Homo sapiens hedgehog interacting protein (HHIP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HHIP ( NM_022475.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_071920.1 Homo sapiens hedgehog interacting protein (HHIP), mRNA.

Price & Availability↑

CloneID	OHu27070
Clone ID Related Accession (Same CDS sequence)	NM_022475.2 , NM_022475.3
Accession Version	NM_022475.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	hedgehog-interacting protein precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI602235.1, AY009951.1 and BC025311.1. This sequence is a reference standard in the RefSeqGene project. On Feb 25, 2011 this sequence version replaced NM_022475.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY009951.1, SRR1660807.35458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGGCAGGT 
ATTCTTGATC AGATCATTGA CATGACATCT TACTTGCTGG ATCTAACAAG TTACATTGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071920.1
CDS	681..2783
Translation	MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISRKHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHRKHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQPPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGPLVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGTSGSCRGYFSGHILGFGEDELGEVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRATVQPAQTLTSECSRLCRNGYCTPTGKCCCSPGWEGDFCRTAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNIRRVTRAGILDQIIDMTSYLLDLTSYIV

Target ORF information:

RefSeq Version	NM_022475.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hedgehog interacting protein (HHIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022475.2

ORF Insert Sequence:

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGGCAGGT 
ATTCTTGATC AGATCATTGA CATGACATCT TACTTGCTGG ATCTAACAAG TTACATTGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HHIP ( NM_022475.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071920.1 Homo sapiens hedgehog interacting protein (HHIP), mRNA.

Price & Availability↑

CloneID	OHu27070
Clone ID Related Accession (Same CDS sequence)	NM_022475.2 , NM_022475.3
Accession Version	NM_022475.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	hedgehog-interacting protein precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY009951.1, BC025311.1 and AC098588.3. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_022475.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY009317.1, SRR1660805.192992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000296575.8/ ENSP00000296575.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGGCAGGT 
ATTCTTGATC AGATCATTGA CATGACATCT TACTTGCTGG ATCTAACAAG TTACATTGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071920.1
CDS	521..2623
Translation	MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISRKHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHRKHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQPPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGPLVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGTSGSCRGYFSGHILGFGEDELGEVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRATVQPAQTLTSECSRLCRNGYCTPTGKCCCSPGWEGDFCRTAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNIRRVTRAGILDQIIDMTSYLLDLTSYIV

Target ORF information:

RefSeq Version	NM_022475.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hedgehog interacting protein (HHIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022475.3

ORF Insert Sequence:

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGGCAGGT 
ATTCTTGATC AGATCATTGA CATGACATCT TACTTGCTGG ATCTAACAAG TTACATTGTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HHIP ( XM_005263178.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263235.1 Homo sapiens hedgehog interacting protein (HHIP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu39052
Clone ID Related Accession (Same CDS sequence)	XM_005263178.4 , XM_005263178.5
Accession Version	XM_005263178.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hedgehog-interacting protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263178.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGGCAGAC 
ATCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263235.1
CDS	550..2598
Translation	MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISRKHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHRKHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQPPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGPLVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGTSGSCRGYFSGHILGFGEDELGEVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRATVQPAQTLTSECSRLCRNGYCTPTGKCCCSPGWEGDFCRTAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNIRRVTRADIT

Target ORF information:

RefSeq Version	XM_005263178.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hedgehog interacting protein (HHIP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263178.4

ORF Insert Sequence:

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGGCAGAC 
ATCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HHIP ( XM_005263178.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263235.1 Homo sapiens hedgehog interacting protein (HHIP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu39052
Clone ID Related Accession (Same CDS sequence)	XM_005263178.4 , XM_005263178.5
Accession Version	XM_005263178.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hedgehog-interacting protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263178.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGGCAGAC 
ATCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263235.1
CDS	523..2571
Translation	MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISRKHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHRKHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQPPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGPLVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGTSGSCRGYFSGHILGFGEDELGEVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRATVQPAQTLTSECSRLCRNGYCTPTGKCCCSPGWEGDFCRTAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNIRRVTRADIT

Target ORF information:

RefSeq Version	XM_005263178.5
Organism	Homo sapiens(human)
Definition	Homo sapiens hedgehog interacting protein (HHIP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263178.5

ORF Insert Sequence:

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGGCAGAC 
ATCACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HHIP ( XM_006714288.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714351.1 Homo sapiens hedgehog interacting protein (HHIP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu39053
Clone ID Related Accession (Same CDS sequence)	XM_006714288.4 , XM_006714288.3
Accession Version	XM_006714288.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	hedgehog-interacting protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006714288.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGACATCA 
CCTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714351.1
CDS	523..2571
Translation	MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISRKHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHRKHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQPPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGPLVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGTSGSCRGYFSGHILGFGEDELGEVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRATVQPAQTLTSECSRLCRNGYCTPTGKCCCSPGWEGDFCRTAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNIRRVTRTSPR

Target ORF information:

RefSeq Version	XM_006714288.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hedgehog interacting protein (HHIP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714288.4

ORF Insert Sequence:

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGACATCA 
CCTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HHIP ( XM_006714288.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714351.1 Homo sapiens hedgehog interacting protein (HHIP), transcript variant X2, mRNA.

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CloneID	OHu39053
Clone ID Related Accession (Same CDS sequence)	XM_006714288.4 , XM_006714288.3
Accession Version	XM_006714288.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	hedgehog-interacting protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714288.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGACATCA 
CCTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714351.1
CDS	550..2598
Translation	MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISRKHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHRKHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQPPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGPLVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGTSGSCRGYFSGHILGFGEDELGEVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRATVQPAQTLTSECSRLCRNGYCTPTGKCCCSPGWEGDFCRTAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNIRRVTRTSPR

Target ORF information:

RefSeq Version	XM_006714288.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hedgehog interacting protein (HHIP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714288.3

ORF Insert Sequence:

ATGCTGAAGA TGCTCTCCTT TAAGCTGCTG CTGCTGGCCG TGGCTCTGGG CTTCTTTGAA 
GGAGATGCTA AGTTTGGGGA AAGAAACGAA GGGAGCGGAG CAAGGAGGAG AAGGTGCCTG 
AATGGGAACC CCCCGAAGCG CCTGAAAAGG AGAGACAGGA GGATGATGTC CCAGCTGGAG 
CTGCTGAGTG GGGGAGAGAT GCTGTGCGGT GGCTTCTACC CTCGGCTGTC CTGCTGCCTG 
CGGAGTGACA GCCCGGGGCT AGGGCGCCTG GAGAATAAGA TATTTTCTGT TACCAACAAC 
ACAGAATGTG GGAAGTTACT GGAGGAAATC AAATGTGCAC TTTGCTCTCC ACATTCTCAA 
AGCCTGTTCC ACTCACCTGA GAGAGAAGTC TTGGAAAGAG ACCTAGTACT TCCTCTGCTC 
TGCAAAGACT ATTGCAAAGA ATTCTTTTAC ACTTGCCGAG GCCATATTCC AGGTTTCCTT 
CAAACAACTG CGGATGAGTT TTGCTTTTAC TATGCAAGAA AAGATGGTGG GTTGTGCTTT 
CCAGATTTTC CAAGAAAACA AGTCAGAGGA CCAGCATCTA ACTACTTGGA CCAGATGGAA 
GAATATGACA AAGTGGAAGA GATCAGCAGA AAGCACAAAC ACAACTGCTT CTGTATTCAG 
GAGGTTGTGA GTGGGCTGCG GCAGCCCGTT GGTGCCCTGC ATAGTGGGGA TGGCTCGCAA 
CGTCTCTTCA TTCTGGAAAA AGAAGGTTAT GTGAAGATAC TTACCCCTGA AGGAGAAATT 
TTCAAGGAGC CTTATTTGGA CATTCACAAA CTTGTTCAAA GTGGAATAAA GGGAGGAGAT 
GAAAGAGGAC TGCTAAGCCT CGCATTCCAT CCCAATTACA AGAAAAATGG AAAGTTGTAT 
GTGTCCTATA CCACCAACCA AGAACGGTGG GCTATCGGGC CTCATGACCA CATTCTTAGG 
GTTGTGGAAT ACACAGTATC CAGAAAAAAT CCACACCAAG TTGATTTGAG AACAGCCAGA 
GTCTTTCTTG AAGTTGCAGA ACTCCACAGA AAGCATCTGG GAGGACAACT GCTCTTTGGC 
CCTGACGGCT TTTTGTACAT CATTCTTGGT GATGGGATGA TTACACTGGA TGATATGGAA 
GAAATGGATG GGTTAAGTGA TTTCACAGGC TCAGTGCTAC GGCTGGATGT GGACACAGAC 
ATGTGCAACG TGCCTTATTC CATACCAAGG AGCAACCCAC ACTTCAACAG CACCAACCAG 
CCCCCCGAAG TGTTTGCTCA TGGGCTCCAC GATCCAGGCA GATGTGCTGT GGATAGACAT 
CCCACTGATA TAAACATCAA TTTAACGATA CTGTGTTCAG ACTCCAATGG AAAAAACAGA 
TCATCAGCCA GAATTCTACA GATAATAAAG GGGAAAGATT ATGAAAGTGA GCCATCACTT 
TTAGAATTCA AGCCATTCAG TAATGGTCCT TTGGTTGGTG GATTTGTATA CCGGGGCTGC 
CAGTCAGAAA GATTGTATGG AAGCTACGTG TTTGGAGATC GTAATGGGAA TTTCCTAACT 
CTCCAGCAAA GTCCTGTGAC AAAGCAGTGG CAAGAAAAAC CACTCTGTCT CGGCACTAGT 
GGGTCCTGTA GAGGCTACTT TTCCGGTCAC ATCTTGGGAT TTGGAGAAGA TGAACTAGGT 
GAAGTTTACA TTTTATCAAG CAGTAAAAGT ATGACCCAGA CTCACAATGG AAAACTCTAC 
AAAATTGTAG ATCCCAAAAG ACCTTTAATG CCTGAGGAAT GCAGAGCCAC GGTACAACCT 
GCACAGACAC TGACTTCAGA GTGCTCCAGG CTCTGTCGAA ACGGCTACTG CACCCCCACG 
GGAAAGTGCT GCTGCAGTCC AGGCTGGGAG GGGGACTTCT GCAGAACTGC AAAATGTGAG 
CCAGCATGTC GTCATGGAGG TGTCTGTGTT AGACCGAACA AGTGCCTCTG TAAAAAAGGA 
TATCTTGGTC CTCAATGTGA ACAAGTGGAC AGAAACATCC GCAGAGTGAC CAGGACATCA 
CCTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparison of the role of HHIP SNPs in susceptibility to chronic obstructive pulmonary disease between Chinese Han and Mongolian populations.
Gene63750-56(2017 Dec)
Xu G,Gao X,Zhang S,Wang Y,Ding M,Liu W,Shen J,Sun D

Hedgehog-Interacting Protein (HIP) Regulates Apoptosis Evasion and Angiogenic Function of Late Endothelial Progenitor Cells.
Scientific reports7(1)12449(2017 Sep)
Lee BNR,Son YS,Lee D,Choi YJ,Kwon SM,Chang HK,Kim PH,Cho JY

Genetic Variants in the Hedgehog Interacting Protein Gene Are Associated with the FEV1/FVC Ratio in Southern Han Chinese Subjects with Chronic Obstructive Pulmonary Disease.
BioMed research international20172756726(2017)
Zhang Z,Wang J,Zheng Z,Chen X,Zeng X,Zhang Y,Li D,Shu J,Yang K,Lai N,Dong L,Lu W

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HHIP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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