ABCG8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ABCG8
Entrez Gene ID	64241
Full Name	ATP binding cassette subfamily G member 8
Synonyms	GBD4,STSL
General protein information	Preferred Names ATP binding cassette subfamily G member 8 Names ATP-binding cassette sub-family G member 8 ATP-binding cassette, sub-family G (WHITE), member 8 sterolin 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p21
Summary	The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008].
Disorder MIM:	605460
Disorder Html:	Sitosterolemia, 210250 (3); {Gallbladder disease 4}, 611465 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001357321.1	NP_001344250.1	ATP-binding cassette sub-family G member 8 isoform 2
XM_011533030.2	XP_011531332.1	ATP-binding cassette sub-family G member 8 isoform X2
XM_005264483.3	XP_005264540.1	ATP-binding cassette sub-family G member 8 isoform X3
NM_022437.2	NP_071882.1	ATP-binding cassette sub-family G member 8 isoform 1
NM_001357321.2	NP_001344250.1	ATP-binding cassette sub-family G member 8 isoform 2
NM_022437.3	NP_071882.1	ATP-binding cassette sub-family G member 8 isoform 1
XM_011533029.2	XP_011531331.1	ATP-binding cassette sub-family G member 8 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABCG8	NP_071882.1
Rattus norvegicus (Norway rat)	Abcg8	NP_569098.2
Saccharomyces cerevisiae (baker's yeast)	YOL075C	NP_014567.2
Kluyveromyces lactis	KLLA0C04477g	XP_452398.1
Magnaporthe oryzae (rice blast fungus)	MGG_10410	XP_003719428.1
Canis lupus familiaris (dog)	ABCG8	XP_531799.3
Mus musculus (house mouse)	Abcg8	NP_080456.1
Xenopus tropicalis (tropical clawed frog)	abcg8	XP_002932437.2
Arabidopsis thaliana (thale cress)	AT2G01320	NP_178241.1
Pan troglodytes (chimpanzee)	ABCG8	XP_525745.2
Gallus gallus (chicken)	ABCG8	XP_419458.4
Oryza sativa (rice)	Os01g0121700	NP_001041878.1
	ABCG8	XP_001111321.2
Bos taurus (cattle)	ABCG8	NP_001019834.1
Danio rerio (zebrafish)	abcg8	NP_001108041.2

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in the ABCG8 gene are associated with sitosterolaemia in the homozygous form and xanthelasmas in the heterozygous form.
Bardawil T, Rebeiz A, Chaabouni M, El Halabi J, Kambris Z, Abbas O, Abou Hassan O, Hamie L, Bitar F, Ghani Kibbi A, Nemer G, Kurban M
European journal of dermatology : EJD27(5)519-523(2017 Oct)

Timely diagnosis of sitosterolemia by next generation sequencing in two children with severe hypercholesterolemia.
Buonuomo PS, Iughetti L, Pisciotta L, Rabacchi C, Papadia F, Bruzzi P, Tummolo A, Bartuli A, Cortese C, Bertolini S, Calandra S
Atherosclerosis26271-77(2017 Jul)

Cholelithiasis in Patients with Gaucher Disease type 1: Risk Factors and the Role of ABCG5/ABCG8 Gene Variants.
Zimmermann A, Popp RA, Al-Khzouz C, Bucerzan S, Na?cu I, Leucuta D, Galle PR, Grigorescu-Sido P
Journal of gastrointestinal and liver diseases : JGLD25(4)447-455(2016 12)

Crystal structure of the human sterol transporter ABCG5/ABCG8.
Lee JY, Kinch LN, Borek DM, Wang J, Wang J, Urbatsch IL, Xie XS, Grishin NV, Cohen JC, Otwinowski Z, Hobbs HH, Rosenbaum DM
Nature533(7604)561-4(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ABCG8 genetic variants may have role in the development of cholelithiasis in patients with Gaucher disease type 1.
Title: Cholelithiasis in Patients with Gaucher Disease type 1: Risk Factors and the Role of ABCG5/ABCG8 Gene Variants.

crystallization in lipid bilayers to determine the X-ray structure of human G5G8 in a nucleotide-free state at 3.9 A resolution, generating the first atomic model of an ABC sterol transporter
Title: Crystal structure of the human sterol transporter ABCG5/ABCG8.

Genetic polymorphism within the ABCG8 gene is a risk factor for diabetes.
Title: ABCG5 and ABCG8 gene polymorphisms in type 2 diabetes mellitus in the Turkish population.

A polymorphism of the sterol transporter ABCG8 has been associated with the prevalence of end-stage renal disease
Title: ABCG8 polymorphisms and renal disease in type 2 diabetic patients.

Mutation in ABCG8 is associated with sitosterolaemia.
Title: Potential effects of NPC1L1 polymorphisms in protecting against clinical disease in a chinese family with sitosterolaemia.

The following ABCG8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABCG8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25026	NM_022437.3 Latest version!	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu35547	NM_001357321.2 Latest version!	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu35547	XM_005264483.3 Latest version!	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu71311	XM_011533029.2 Latest version!	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu71312	XM_011533030.2 Latest version!	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu35547	NM_001357321.1	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu25026	NM_022437.2	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABCG8 ( NM_022437.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_071882.1 Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25026
Clone ID Related Accession (Same CDS sequence)	NM_022437.2 , NM_022437.3
Accession Version	NM_022437.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 8 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Karyn Megy. The reference sequence was derived from AF320294.1 and AF324494.1. This sequence is a reference standard in the RefSeqGene project. On Sep 4, 2003 this sequence version replaced NM_022437.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF324494.1, BC113657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCAG CGTGACCCCA 
CTAGACACCA ACTGCCTCCC GAGTCCTACG AAGATGCCTG GGGCGGTGCA GCAGTTTACG 
ACGCTGATCC GTCGTCAGAT TTCCAACGAC TTCCGAGACC TGCCCACCCT CCTCATCCAT 
GGGGCGGAGG CCTGTCTGAT GTCAATGACC ATCGGCTTCC TCTATTTTGG CCATGGGAGC 
ATCCAGCTCT CCTTCATGGA TACAGCCGCC CTCTTGTTCA TGATCGGTGC TCTCATCCCT 
TTCAACGTCA TTCTGGATGT CATCTCCAAA TGTTACTCAG AGAGGGCAAT GCTTTACTAT 
GAACTGGAAG ACGGGCTGTA CACCACTGGT CCATATTTCT TTGCCAAGAT CCTCGGGGAG 
CTTCCGGAGC ACTGTGCCTA CATCATCATC TACGGGATGC CCACCTACTG GCTGGCCAAC 
CTGAGGCCAG GCCTCCAGCC CTTCCTGCTG CACTTCCTGC TGGTGTGGCT GGTGGTCTTC 
TGTTGCAGGA TTATGGCCCT GGCCGCCGCG GCCCTGCTCC CCACCTTCCA CATGGCCTCC 
TTCTTCAGCA ATGCCCTCTA CAACTCCTTC TACCTCGCCG GGGGCTTCAT GATAAACTTG 
AGCAGCCTGT GGACAGTGCC CGCGTGGATT TCCAAAGTGT CCTTCCTGCG GTGGTGTTTT 
GAAGGGCTGA TGAAGATTCA GTTCAGCAGA AGAACTTATA AAATGCCTCT CGGGAACCTC 
ACCATCGCGG TCTCAGGAGA TAAAATCCTC AGTGTCATGG AGCTGGACTC GTACCCTCTC 
TACGCCATCT ACCTCATCGT CATTGGCCTC AGCGGTGGCT TCATGGTCCT GTACTACGTG 
TCCTTAAGGT TCATCAAACA GAAACCAAGT CAAGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071882.1
CDS	91..2112
Translation	MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW

Target ORF information:

RefSeq Version	NM_022437.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022437.2

ORF Insert Sequence:

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCAG CGTGACCCCA 
CTAGACACCA ACTGCCTCCC GAGTCCTACG AAGATGCCTG GGGCGGTGCA GCAGTTTACG 
ACGCTGATCC GTCGTCAGAT TTCCAACGAC TTCCGAGACC TGCCCACCCT CCTCATCCAT 
GGGGCGGAGG CCTGTCTGAT GTCAATGACC ATCGGCTTCC TCTATTTTGG CCATGGGAGC 
ATCCAGCTCT CCTTCATGGA TACAGCCGCC CTCTTGTTCA TGATCGGTGC TCTCATCCCT 
TTCAACGTCA TTCTGGATGT CATCTCCAAA TGTTACTCAG AGAGGGCAAT GCTTTACTAT 
GAACTGGAAG ACGGGCTGTA CACCACTGGT CCATATTTCT TTGCCAAGAT CCTCGGGGAG 
CTTCCGGAGC ACTGTGCCTA CATCATCATC TACGGGATGC CCACCTACTG GCTGGCCAAC 
CTGAGGCCAG GCCTCCAGCC CTTCCTGCTG CACTTCCTGC TGGTGTGGCT GGTGGTCTTC 
TGTTGCAGGA TTATGGCCCT GGCCGCCGCG GCCCTGCTCC CCACCTTCCA CATGGCCTCC 
TTCTTCAGCA ATGCCCTCTA CAACTCCTTC TACCTCGCCG GGGGCTTCAT GATAAACTTG 
AGCAGCCTGT GGACAGTGCC CGCGTGGATT TCCAAAGTGT CCTTCCTGCG GTGGTGTTTT 
GAAGGGCTGA TGAAGATTCA GTTCAGCAGA AGAACTTATA AAATGCCTCT CGGGAACCTC 
ACCATCGCGG TCTCAGGAGA TAAAATCCTC AGTGTCATGG AGCTGGACTC GTACCCTCTC 
TACGCCATCT ACCTCATCGT CATTGGCCTC AGCGGTGGCT TCATGGTCCT GTACTACGTG 
TCCTTAAGGT TCATCAAACA GAAACCAAGT CAAGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG8 ( NM_022437.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071882.1 Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25026
Clone ID Related Accession (Same CDS sequence)	NM_022437.2 , NM_022437.3
Accession Version	NM_022437.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 8 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Karyn Megy. The reference sequence was derived from AC108476.5. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2018 this sequence version replaced NM_022437.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF324494.1, BC113657.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000272286.4/ ENSP00000272286.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCAG CGTGACCCCA 
CTAGACACCA ACTGCCTCCC GAGTCCTACG AAGATGCCTG GGGCGGTGCA GCAGTTTACG 
ACGCTGATCC GTCGTCAGAT TTCCAACGAC TTCCGAGACC TGCCCACCCT CCTCATCCAT 
GGGGCGGAGG CCTGTCTGAT GTCAATGACC ATCGGCTTCC TCTATTTTGG CCATGGGAGC 
ATCCAGCTCT CCTTCATGGA TACAGCCGCC CTCTTGTTCA TGATCGGTGC TCTCATCCCT 
TTCAACGTCA TTCTGGATGT CATCTCCAAA TGTTACTCAG AGAGGGCAAT GCTTTACTAT 
GAACTGGAAG ACGGGCTGTA CACCACTGGT CCATATTTCT TTGCCAAGAT CCTCGGGGAG 
CTTCCGGAGC ACTGTGCCTA CATCATCATC TACGGGATGC CCACCTACTG GCTGGCCAAC 
CTGAGGCCAG GCCTCCAGCC CTTCCTGCTG CACTTCCTGC TGGTGTGGCT GGTGGTCTTC 
TGTTGCAGGA TTATGGCCCT GGCCGCCGCG GCCCTGCTCC CCACCTTCCA CATGGCCTCC 
TTCTTCAGCA ATGCCCTCTA CAACTCCTTC TACCTCGCCG GGGGCTTCAT GATAAACTTG 
AGCAGCCTGT GGACAGTGCC CGCGTGGATT TCCAAAGTGT CCTTCCTGCG GTGGTGTTTT 
GAAGGGCTGA TGAAGATTCA GTTCAGCAGA AGAACTTATA AAATGCCTCT CGGGAACCTC 
ACCATCGCGG TCTCAGGAGA TAAAATCCTC AGTGTCATGG AGCTGGACTC GTACCCTCTC 
TACGCCATCT ACCTCATCGT CATTGGCCTC AGCGGTGGCT TCATGGTCCT GTACTACGTG 
TCCTTAAGGT TCATCAAACA GAAACCAAGT CAAGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071882.1
CDS	84..2105
Translation	MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW

Target ORF information:

RefSeq Version	NM_022437.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022437.3

ORF Insert Sequence:

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCAG CGTGACCCCA 
CTAGACACCA ACTGCCTCCC GAGTCCTACG AAGATGCCTG GGGCGGTGCA GCAGTTTACG 
ACGCTGATCC GTCGTCAGAT TTCCAACGAC TTCCGAGACC TGCCCACCCT CCTCATCCAT 
GGGGCGGAGG CCTGTCTGAT GTCAATGACC ATCGGCTTCC TCTATTTTGG CCATGGGAGC 
ATCCAGCTCT CCTTCATGGA TACAGCCGCC CTCTTGTTCA TGATCGGTGC TCTCATCCCT 
TTCAACGTCA TTCTGGATGT CATCTCCAAA TGTTACTCAG AGAGGGCAAT GCTTTACTAT 
GAACTGGAAG ACGGGCTGTA CACCACTGGT CCATATTTCT TTGCCAAGAT CCTCGGGGAG 
CTTCCGGAGC ACTGTGCCTA CATCATCATC TACGGGATGC CCACCTACTG GCTGGCCAAC 
CTGAGGCCAG GCCTCCAGCC CTTCCTGCTG CACTTCCTGC TGGTGTGGCT GGTGGTCTTC 
TGTTGCAGGA TTATGGCCCT GGCCGCCGCG GCCCTGCTCC CCACCTTCCA CATGGCCTCC 
TTCTTCAGCA ATGCCCTCTA CAACTCCTTC TACCTCGCCG GGGGCTTCAT GATAAACTTG 
AGCAGCCTGT GGACAGTGCC CGCGTGGATT TCCAAAGTGT CCTTCCTGCG GTGGTGTTTT 
GAAGGGCTGA TGAAGATTCA GTTCAGCAGA AGAACTTATA AAATGCCTCT CGGGAACCTC 
ACCATCGCGG TCTCAGGAGA TAAAATCCTC AGTGTCATGG AGCTGGACTC GTACCCTCTC 
TACGCCATCT ACCTCATCGT CATTGGCCTC AGCGGTGGCT TCATGGTCCT GTACTACGTG 
TCCTTAAGGT TCATCAAACA GAAACCAAGT CAAGACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG8 ( NM_001357321.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001344250.1 Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 2, mRNA.

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CloneID	OHu35547
Clone ID Related Accession (Same CDS sequence)	NM_001357321.1 , XM_005264483.3 , NM_001357321.2
Accession Version	NM_001357321.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 8 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF324494.1, BC143564.1 and AC108476.5. On Oct 24, 2017 this sequence version replaced XM_005264483.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCGT GACCCCACTA 
GACACCAACT GCCTCCCGAG TCCTACGAAG ATGCCTGGGG CGGTGCAGCA GTTTACGACG 
CTGATCCGTC GTCAGATTTC CAACGACTTC CGAGACCTGC CCACCCTCCT CATCCATGGG 
GCGGAGGCCT GTCTGATGTC AATGACCATC GGCTTCCTCT ATTTTGGCCA TGGGAGCATC 
CAGCTCTCCT TCATGGATAC AGCCGCCCTC TTGTTCATGA TCGGTGCTCT CATCCCTTTC 
AACGTCATTC TGGATGTCAT CTCCAAATGT TACTCAGAGA GGGCAATGCT TTACTATGAA 
CTGGAAGACG GGCTGTACAC CACTGGTCCA TATTTCTTTG CCAAGATCCT CGGGGAGCTT 
CCGGAGCACT GTGCCTACAT CATCATCTAC GGGATGCCCA CCTACTGGCT GGCCAACCTG 
AGGCCAGGCC TCCAGCCCTT CCTGCTGCAC TTCCTGCTGG TGTGGCTGGT GGTCTTCTGT 
TGCAGGATTA TGGCCCTGGC CGCCGCGGCC CTGCTCCCCA CCTTCCACAT GGCCTCCTTC 
TTCAGCAATG CCCTCTACAA CTCCTTCTAC CTCGCCGGGG GCTTCATGAT AAACTTGAGC 
AGCCTGTGGA CAGTGCCCGC GTGGATTTCC AAAGTGTCCT TCCTGCGGTG GTGTTTTGAA 
GGGCTGATGA AGATTCAGTT CAGCAGAAGA ACTTATAAAA TGCCTCTCGG GAACCTCACC 
ATCGCGGTCT CAGGAGATAA AATCCTCAGT GTCATGGAGC TGGACTCGTA CCCTCTCTAC 
GCCATCTACC TCATCGTCAT TGGCCTCAGC GGTGGCTTCA TGGTCCTGTA CTACGTGTCC 
TTAAGGTTCA TCAAACAGAA ACCAAGTCAA GACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344250.1
CDS	91..2109
Translation	MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW

Target ORF information:

RefSeq Version	NM_001357321.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357321.1

ORF Insert Sequence:

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCGT GACCCCACTA 
GACACCAACT GCCTCCCGAG TCCTACGAAG ATGCCTGGGG CGGTGCAGCA GTTTACGACG 
CTGATCCGTC GTCAGATTTC CAACGACTTC CGAGACCTGC CCACCCTCCT CATCCATGGG 
GCGGAGGCCT GTCTGATGTC AATGACCATC GGCTTCCTCT ATTTTGGCCA TGGGAGCATC 
CAGCTCTCCT TCATGGATAC AGCCGCCCTC TTGTTCATGA TCGGTGCTCT CATCCCTTTC 
AACGTCATTC TGGATGTCAT CTCCAAATGT TACTCAGAGA GGGCAATGCT TTACTATGAA 
CTGGAAGACG GGCTGTACAC CACTGGTCCA TATTTCTTTG CCAAGATCCT CGGGGAGCTT 
CCGGAGCACT GTGCCTACAT CATCATCTAC GGGATGCCCA CCTACTGGCT GGCCAACCTG 
AGGCCAGGCC TCCAGCCCTT CCTGCTGCAC TTCCTGCTGG TGTGGCTGGT GGTCTTCTGT 
TGCAGGATTA TGGCCCTGGC CGCCGCGGCC CTGCTCCCCA CCTTCCACAT GGCCTCCTTC 
TTCAGCAATG CCCTCTACAA CTCCTTCTAC CTCGCCGGGG GCTTCATGAT AAACTTGAGC 
AGCCTGTGGA CAGTGCCCGC GTGGATTTCC AAAGTGTCCT TCCTGCGGTG GTGTTTTGAA 
GGGCTGATGA AGATTCAGTT CAGCAGAAGA ACTTATAAAA TGCCTCTCGG GAACCTCACC 
ATCGCGGTCT CAGGAGATAA AATCCTCAGT GTCATGGAGC TGGACTCGTA CCCTCTCTAC 
GCCATCTACC TCATCGTCAT TGGCCTCAGC GGTGGCTTCA TGGTCCTGTA CTACGTGTCC 
TTAAGGTTCA TCAAACAGAA ACCAAGTCAA GACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG8 ( NM_001357321.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001344250.1 Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 2, mRNA.

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CloneID	OHu35547
Clone ID Related Accession (Same CDS sequence)	NM_001357321.1 , XM_005264483.3 , NM_001357321.2
Accession Version	NM_001357321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-20
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 8 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF324494.1, BC143564.1 and AC108476.5. On May 31, 2019 this sequence version replaced NM_001357321.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC143564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835, SAMEA2152474 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCGT GACCCCACTA 
GACACCAACT GCCTCCCGAG TCCTACGAAG ATGCCTGGGG CGGTGCAGCA GTTTACGACG 
CTGATCCGTC GTCAGATTTC CAACGACTTC CGAGACCTGC CCACCCTCCT CATCCATGGG 
GCGGAGGCCT GTCTGATGTC AATGACCATC GGCTTCCTCT ATTTTGGCCA TGGGAGCATC 
CAGCTCTCCT TCATGGATAC AGCCGCCCTC TTGTTCATGA TCGGTGCTCT CATCCCTTTC 
AACGTCATTC TGGATGTCAT CTCCAAATGT TACTCAGAGA GGGCAATGCT TTACTATGAA 
CTGGAAGACG GGCTGTACAC CACTGGTCCA TATTTCTTTG CCAAGATCCT CGGGGAGCTT 
CCGGAGCACT GTGCCTACAT CATCATCTAC GGGATGCCCA CCTACTGGCT GGCCAACCTG 
AGGCCAGGCC TCCAGCCCTT CCTGCTGCAC TTCCTGCTGG TGTGGCTGGT GGTCTTCTGT 
TGCAGGATTA TGGCCCTGGC CGCCGCGGCC CTGCTCCCCA CCTTCCACAT GGCCTCCTTC 
TTCAGCAATG CCCTCTACAA CTCCTTCTAC CTCGCCGGGG GCTTCATGAT AAACTTGAGC 
AGCCTGTGGA CAGTGCCCGC GTGGATTTCC AAAGTGTCCT TCCTGCGGTG GTGTTTTGAA 
GGGCTGATGA AGATTCAGTT CAGCAGAAGA ACTTATAAAA TGCCTCTCGG GAACCTCACC 
ATCGCGGTCT CAGGAGATAA AATCCTCAGT GTCATGGAGC TGGACTCGTA CCCTCTCTAC 
GCCATCTACC TCATCGTCAT TGGCCTCAGC GGTGGCTTCA TGGTCCTGTA CTACGTGTCC 
TTAAGGTTCA TCAAACAGAA ACCAAGTCAA GACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344250.1
CDS	84..2102
Translation	MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW

Target ORF information:

RefSeq Version	NM_001357321.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357321.2

ORF Insert Sequence:

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCGT GACCCCACTA 
GACACCAACT GCCTCCCGAG TCCTACGAAG ATGCCTGGGG CGGTGCAGCA GTTTACGACG 
CTGATCCGTC GTCAGATTTC CAACGACTTC CGAGACCTGC CCACCCTCCT CATCCATGGG 
GCGGAGGCCT GTCTGATGTC AATGACCATC GGCTTCCTCT ATTTTGGCCA TGGGAGCATC 
CAGCTCTCCT TCATGGATAC AGCCGCCCTC TTGTTCATGA TCGGTGCTCT CATCCCTTTC 
AACGTCATTC TGGATGTCAT CTCCAAATGT TACTCAGAGA GGGCAATGCT TTACTATGAA 
CTGGAAGACG GGCTGTACAC CACTGGTCCA TATTTCTTTG CCAAGATCCT CGGGGAGCTT 
CCGGAGCACT GTGCCTACAT CATCATCTAC GGGATGCCCA CCTACTGGCT GGCCAACCTG 
AGGCCAGGCC TCCAGCCCTT CCTGCTGCAC TTCCTGCTGG TGTGGCTGGT GGTCTTCTGT 
TGCAGGATTA TGGCCCTGGC CGCCGCGGCC CTGCTCCCCA CCTTCCACAT GGCCTCCTTC 
TTCAGCAATG CCCTCTACAA CTCCTTCTAC CTCGCCGGGG GCTTCATGAT AAACTTGAGC 
AGCCTGTGGA CAGTGCCCGC GTGGATTTCC AAAGTGTCCT TCCTGCGGTG GTGTTTTGAA 
GGGCTGATGA AGATTCAGTT CAGCAGAAGA ACTTATAAAA TGCCTCTCGG GAACCTCACC 
ATCGCGGTCT CAGGAGATAA AATCCTCAGT GTCATGGAGC TGGACTCGTA CCCTCTCTAC 
GCCATCTACC TCATCGTCAT TGGCCTCAGC GGTGGCTTCA TGGTCCTGTA CTACGTGTCC 
TTAAGGTTCA TCAAACAGAA ACCAAGTCAA GACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG8 ( XM_005264483.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264540.1 Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant X5, mRNA.

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CloneID	OHu35547
Clone ID Related Accession (Same CDS sequence)	NM_001357321.1 , XM_005264483.3 , NM_001357321.2
Accession Version	XM_005264483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	ATP-binding cassette sub-family G member 8 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCGT GACCCCACTA 
GACACCAACT GCCTCCCGAG TCCTACGAAG ATGCCTGGGG CGGTGCAGCA GTTTACGACG 
CTGATCCGTC GTCAGATTTC CAACGACTTC CGAGACCTGC CCACCCTCCT CATCCATGGG 
GCGGAGGCCT GTCTGATGTC AATGACCATC GGCTTCCTCT ATTTTGGCCA TGGGAGCATC 
CAGCTCTCCT TCATGGATAC AGCCGCCCTC TTGTTCATGA TCGGTGCTCT CATCCCTTTC 
AACGTCATTC TGGATGTCAT CTCCAAATGT TACTCAGAGA GGGCAATGCT TTACTATGAA 
CTGGAAGACG GGCTGTACAC CACTGGTCCA TATTTCTTTG CCAAGATCCT CGGGGAGCTT 
CCGGAGCACT GTGCCTACAT CATCATCTAC GGGATGCCCA CCTACTGGCT GGCCAACCTG 
AGGCCAGGCC TCCAGCCCTT CCTGCTGCAC TTCCTGCTGG TGTGGCTGGT GGTCTTCTGT 
TGCAGGATTA TGGCCCTGGC CGCCGCGGCC CTGCTCCCCA CCTTCCACAT GGCCTCCTTC 
TTCAGCAATG CCCTCTACAA CTCCTTCTAC CTCGCCGGGG GCTTCATGAT AAACTTGAGC 
AGCCTGTGGA CAGTGCCCGC GTGGATTTCC AAAGTGTCCT TCCTGCGGTG GTGTTTTGAA 
GGGCTGATGA AGATTCAGTT CAGCAGAAGA ACTTATAAAA TGCCTCTCGG GAACCTCACC 
ATCGCGGTCT CAGGAGATAA AATCCTCAGT GTCATGGAGC TGGACTCGTA CCCTCTCTAC 
GCCATCTACC TCATCGTCAT TGGCCTCAGC GGTGGCTTCA TGGTCCTGTA CTACGTGTCC 
TTAAGGTTCA TCAAACAGAA ACCAAGTCAA GACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264540.1
CDS	206..2224
Translation	MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW

Target ORF information:

RefSeq Version	XM_005264483.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264483.3

ORF Insert Sequence:

ATGGCCGGGA AGGCGGCAGA GGAGAGAGGG CTGCCGAAAG GGGCCACTCC CCAGGATACC 
TCGGGCCTCC AGGATAGATT GTTCTCCTCT GAAAGTGACA ACAGCCTGTA CTTCACCTAC 
AGTGGCCAGC CCAACACCCT GGAGGTCAGA GACCTCAACT ACCAGGTGGA CCTGGCCTCT 
CAGGTCCCTT GGTTTGAGCA GCTGGCTCAG TTCAAGATGC CCTGGACATC TCCCAGCTGC 
CAGAATTCTT GTGAGCTGGG CATCCAGAAC CTAAGCTTCA AAGTGAGAAG TGGGCAGATG 
CTGGCCATCA TAGGGAGCTC AGGTTGTGGG AGAGCCTCCT TGCTAGATGT GATCACTGGC 
CGAGGTCACG GCGGCAAGAT CAAGTCAGGC CAGATCTGGA TCAATGGGCA GCCCAGCTCG 
CCTCAGCTGG TGAGGAAGTG TGTGGCCCAC GTGCGCCAGC ACAACCAGCT GCTCCCCAAC 
TTGACTGTGC GAGAGACCTT GGCCTTCATT GCCCAGATGC GGCTGCCCAG AACCTTCTCC 
CAGGCCCAGC GTGACAAAAG GGTGGAGGAC GTGATCGCGG AGCTGCGGCT TAGGCAGTGC 
GCTGACACCC GCGTGGGCAA CATGTACGTG CGGGGGTTGT CGGGGGGTGA GCGCAGGAGA 
GTCAGCATTG GGGTGCAGCT CCTGTGGAAC CCAGGAATCC TTATTCTCGA CGAACCCACC 
TCTGGGCTCG ACAGCTTCAC AGCCCACAAC CTGGTGAAGA CCTTGTCCAG GCTGGCCAAA 
GGCAACCGGC TGGTGCTCAT CTCCCTCCAC CAGCCTCGCT CTGACATCTT CAGGCTGTTT 
GATCTGGTCC TCCTGATGAC GTCTGGCACC CCCATCTACT TAGGGGCGGC CCAGCACATG 
GTCCAGTATT TCACAGCCAT CGGCTACCCC TGTCCTCGCT ACAGCAATCC TGCTGACTTC 
TATGTGGACC TGACCAGCAT TGACAGGCGC AGCAGAGAGC AGGAATTGGC CACCAGGGAG 
AAGGCTCAGT CACTCGCAGC CCTGTTTCTA GAAAAAGTGC GTGACTTAGA TGACTTTCTA 
TGGAAAGCAG AGACGAAGGA TCTTGACGAG GACACCTGTG TGGAAAGCGT GACCCCACTA 
GACACCAACT GCCTCCCGAG TCCTACGAAG ATGCCTGGGG CGGTGCAGCA GTTTACGACG 
CTGATCCGTC GTCAGATTTC CAACGACTTC CGAGACCTGC CCACCCTCCT CATCCATGGG 
GCGGAGGCCT GTCTGATGTC AATGACCATC GGCTTCCTCT ATTTTGGCCA TGGGAGCATC 
CAGCTCTCCT TCATGGATAC AGCCGCCCTC TTGTTCATGA TCGGTGCTCT CATCCCTTTC 
AACGTCATTC TGGATGTCAT CTCCAAATGT TACTCAGAGA GGGCAATGCT TTACTATGAA 
CTGGAAGACG GGCTGTACAC CACTGGTCCA TATTTCTTTG CCAAGATCCT CGGGGAGCTT 
CCGGAGCACT GTGCCTACAT CATCATCTAC GGGATGCCCA CCTACTGGCT GGCCAACCTG 
AGGCCAGGCC TCCAGCCCTT CCTGCTGCAC TTCCTGCTGG TGTGGCTGGT GGTCTTCTGT 
TGCAGGATTA TGGCCCTGGC CGCCGCGGCC CTGCTCCCCA CCTTCCACAT GGCCTCCTTC 
TTCAGCAATG CCCTCTACAA CTCCTTCTAC CTCGCCGGGG GCTTCATGAT AAACTTGAGC 
AGCCTGTGGA CAGTGCCCGC GTGGATTTCC AAAGTGTCCT TCCTGCGGTG GTGTTTTGAA 
GGGCTGATGA AGATTCAGTT CAGCAGAAGA ACTTATAAAA TGCCTCTCGG GAACCTCACC 
ATCGCGGTCT CAGGAGATAA AATCCTCAGT GTCATGGAGC TGGACTCGTA CCCTCTCTAC 
GCCATCTACC TCATCGTCAT TGGCCTCAGC GGTGGCTTCA TGGTCCTGTA CTACGTGTCC 
TTAAGGTTCA TCAAACAGAA ACCAAGTCAA GACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG8 ( XM_011533029.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531331.1 Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant X1, mRNA.

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CloneID	OHu71311
Clone ID Related Accession (Same CDS sequence)	XM_011533029.2
Accession Version	XM_011533029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGCC TCAAGCCGCA CCCAAGACCT GCTGAGTCAG CTCTGCTTCA GTCGAGTTTG 
AGAAGCACTG GCTTGGGCCT CCAGGATAGA TTGTTCTCCT CTGAAAGTGA CAACAGCCTG 
TACTTCACCT ACAGTGGCCA GCCCAACACC CTGGAGGTCA GAGACCTCAA CTACCAGGTG 
GACCTGGCCT CTCAGGTCCC TTGGTTTGAG CAGCTGGCTC AGTTCAAGAT GCCCTGGACA 
TCTCCCAGCT GCCAGAATTC TTGTGAGCTG GGCATCCAGA ACCTAAGCTT CAAAGTGAGA 
AGTGGGCAGA TGCTGGCCAT CATAGGGAGC TCAGGTTGTG GGAGAGCCTC CTTGCTAGAT 
GTGATCACTG GCCGAGGTCA CGGCGGCAAG ATCAAGTCAG GCCAGATCTG GATCAATGGG 
CAGCCCAGCT CGCCTCAGCT GGTGAGGAAG TGTGTGGCCC ACGTGCGCCA GCACAACCAG 
CTGCTCCCCA ACTTGACTGT GCGAGAGACC TTGGCCTTCA TTGCCCAGAT GCGGCTGCCC 
AGAACCTTCT CCCAGGCCCA GCGTGACAAA AGGGTGGAGG ACGTGATCGC GGAGCTGCGG 
CTTAGGCAGT GCGCTGACAC CCGCGTGGGC AACATGTACG TGCGGGGGTT GTCGGGGGGT 
GAGCGCAGGA GAGTCAGCAT TGGGGTGCAG CTCCTGTGGA ACCCAGGAAT CCTTATTCTC 
GACGAACCCA CCTCTGGGCT CGACAGCTTC ACAGCCCACA ACCTGGTGAA GACCTTGTCC 
AGGCTGGCCA AAGGCAACCG GCTGGTGCTC ATCTCCCTCC ACCAGCCTCG CTCTGACATC 
TTCAGGCTGT TTGATCTGGT CCTCCTGATG ACGTCTGGCA CCCCCATCTA CTTAGGGGCG 
GCCCAGCACA TGGTCCAGTA TTTCACAGCC ATCGGCTACC CCTGTCCTCG CTACAGCAAT 
CCTGCTGACT TCTATGTGGA CCTGACCAGC ATTGACAGGC GCAGCAGAGA GCAGGAATTG 
GCCACCAGGG AGAAGGCTCA GTCACTCGCA GCCCTGTTTC TAGAAAAAGT GCGTGACTTA 
GATGACTTTC TATGGAAAGC AGAGACGAAG GATCTTGACG AGGACACCTG TGTGGAAAGC 
AGCGTGACCC CACTAGACAC CAACTGCCTC CCGAGTCCTA CGAAGATGCC TGGGGCGGTG 
CAGCAGTTTA CGACGCTGAT CCGTCGTCAG ATTTCCAACG ACTTCCGAGA CCTGCCCACC 
CTCCTCATCC ATGGGGCGGA GGCCTGTCTG ATGTCAATGA CCATCGGCTT CCTCTATTTT 
GGCCATGGGA GCATCCAGCT CTCCTTCATG GATACAGCCG CCCTCTTGTT CATGATCGGT 
GCTCTCATCC CTTTCAACGT CATTCTGGAT GTCATCTCCA AATGTTACTC AGAGAGGGCA 
ATGCTTTACT ATGAACTGGA AGACGGGCTG TACACCACTG GTCCATATTT CTTTGCCAAG 
ATCCTCGGGG AGCTTCCGGA GCACTGTGCC TACATCATCA TCTACGGGAT GCCCACCTAC 
TGGCTGGCCA ACCTGAGGCC AGGCCTCCAG CCCTTCCTGC TGCACTTCCT GCTGGTGTGG 
CTGGTGGTCT TCTGTTGCAG GATTATGGCC CTGGCCGCCG CGGCCCTGCT CCCCACCTTC 
CACATGGCCT CCTTCTTCAG CAATGCCCTC TACAACTCCT TCTACCTCGC CGGGGGCTTC 
ATGATAAACT TGAGCAGCCT GTGGACAGTG CCCGCGTGGA TTTCCAAAGT GTCCTTCCTG 
CGGTGGTGTT TTGAAGGGCT GATGAAGATT CAGTTCAGCA GAAGAACTTA TAAAATGCCT 
CTCGGGAACC TCACCATCGC GGTCTCAGGA GATAAAATCC TCAGTGTCAT GGAGCTGGAC 
TCGTACCCTC TCTACGCCAT CTACCTCATC GTCATTGGCC TCAGCGGTGG CTTCATGGTC 
CTGTACTACG TGTCCTTAAG GTTCATCAAA CAGAAACCAA GTCAAGACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531331.1
CDS	646..2679
Translation	MQSLKPHPRPAESALLQSSLRSTGLGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW

Target ORF information:

RefSeq Version	XM_011533029.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533029.2

ORF Insert Sequence:

ATGCAGAGCC TCAAGCCGCA CCCAAGACCT GCTGAGTCAG CTCTGCTTCA GTCGAGTTTG 
AGAAGCACTG GCTTGGGCCT CCAGGATAGA TTGTTCTCCT CTGAAAGTGA CAACAGCCTG 
TACTTCACCT ACAGTGGCCA GCCCAACACC CTGGAGGTCA GAGACCTCAA CTACCAGGTG 
GACCTGGCCT CTCAGGTCCC TTGGTTTGAG CAGCTGGCTC AGTTCAAGAT GCCCTGGACA 
TCTCCCAGCT GCCAGAATTC TTGTGAGCTG GGCATCCAGA ACCTAAGCTT CAAAGTGAGA 
AGTGGGCAGA TGCTGGCCAT CATAGGGAGC TCAGGTTGTG GGAGAGCCTC CTTGCTAGAT 
GTGATCACTG GCCGAGGTCA CGGCGGCAAG ATCAAGTCAG GCCAGATCTG GATCAATGGG 
CAGCCCAGCT CGCCTCAGCT GGTGAGGAAG TGTGTGGCCC ACGTGCGCCA GCACAACCAG 
CTGCTCCCCA ACTTGACTGT GCGAGAGACC TTGGCCTTCA TTGCCCAGAT GCGGCTGCCC 
AGAACCTTCT CCCAGGCCCA GCGTGACAAA AGGGTGGAGG ACGTGATCGC GGAGCTGCGG 
CTTAGGCAGT GCGCTGACAC CCGCGTGGGC AACATGTACG TGCGGGGGTT GTCGGGGGGT 
GAGCGCAGGA GAGTCAGCAT TGGGGTGCAG CTCCTGTGGA ACCCAGGAAT CCTTATTCTC 
GACGAACCCA CCTCTGGGCT CGACAGCTTC ACAGCCCACA ACCTGGTGAA GACCTTGTCC 
AGGCTGGCCA AAGGCAACCG GCTGGTGCTC ATCTCCCTCC ACCAGCCTCG CTCTGACATC 
TTCAGGCTGT TTGATCTGGT CCTCCTGATG ACGTCTGGCA CCCCCATCTA CTTAGGGGCG 
GCCCAGCACA TGGTCCAGTA TTTCACAGCC ATCGGCTACC CCTGTCCTCG CTACAGCAAT 
CCTGCTGACT TCTATGTGGA CCTGACCAGC ATTGACAGGC GCAGCAGAGA GCAGGAATTG 
GCCACCAGGG AGAAGGCTCA GTCACTCGCA GCCCTGTTTC TAGAAAAAGT GCGTGACTTA 
GATGACTTTC TATGGAAAGC AGAGACGAAG GATCTTGACG AGGACACCTG TGTGGAAAGC 
AGCGTGACCC CACTAGACAC CAACTGCCTC CCGAGTCCTA CGAAGATGCC TGGGGCGGTG 
CAGCAGTTTA CGACGCTGAT CCGTCGTCAG ATTTCCAACG ACTTCCGAGA CCTGCCCACC 
CTCCTCATCC ATGGGGCGGA GGCCTGTCTG ATGTCAATGA CCATCGGCTT CCTCTATTTT 
GGCCATGGGA GCATCCAGCT CTCCTTCATG GATACAGCCG CCCTCTTGTT CATGATCGGT 
GCTCTCATCC CTTTCAACGT CATTCTGGAT GTCATCTCCA AATGTTACTC AGAGAGGGCA 
ATGCTTTACT ATGAACTGGA AGACGGGCTG TACACCACTG GTCCATATTT CTTTGCCAAG 
ATCCTCGGGG AGCTTCCGGA GCACTGTGCC TACATCATCA TCTACGGGAT GCCCACCTAC 
TGGCTGGCCA ACCTGAGGCC AGGCCTCCAG CCCTTCCTGC TGCACTTCCT GCTGGTGTGG 
CTGGTGGTCT TCTGTTGCAG GATTATGGCC CTGGCCGCCG CGGCCCTGCT CCCCACCTTC 
CACATGGCCT CCTTCTTCAG CAATGCCCTC TACAACTCCT TCTACCTCGC CGGGGGCTTC 
ATGATAAACT TGAGCAGCCT GTGGACAGTG CCCGCGTGGA TTTCCAAAGT GTCCTTCCTG 
CGGTGGTGTT TTGAAGGGCT GATGAAGATT CAGTTCAGCA GAAGAACTTA TAAAATGCCT 
CTCGGGAACC TCACCATCGC GGTCTCAGGA GATAAAATCC TCAGTGTCAT GGAGCTGGAC 
TCGTACCCTC TCTACGCCAT CTACCTCATC GTCATTGGCC TCAGCGGTGG CTTCATGGTC 
CTGTACTACG TGTCCTTAAG GTTCATCAAA CAGAAACCAA GTCAAGACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCG8 ( XM_011533030.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531332.1 Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu71312
Clone ID Related Accession (Same CDS sequence)	XM_011533030.2
Accession Version	XM_011533030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-binding cassette sub-family G member 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGCC TCAAGCCGCA CCCAAGACCT GCTGAGTCAG CTCTGCTTCA GTCGAGTTTG 
AGAAGCACTG GCTTGGGCCT CCAGGATAGA TTGTTCTCCT CTGAAAGTGA CAACAGCCTG 
TACTTCACCT ACAGTGGCCA GCCCAACACC CTGGAGGTCA GAGACCTCAA CTACCAGGTG 
GACCTGGCCT CTCAGGTCCC TTGGTTTGAG CAGCTGGCTC AGTTCAAGAT GCCCTGGACA 
TCTCCCAGCT GCCAGAATTC TTGTGAGCTG GGCATCCAGA ACCTAAGCTT CAAAGTGAGA 
AGTGGGCAGA TGCTGGCCAT CATAGGGAGC TCAGGTTGTG GGAGAGCCTC CTTGCTAGAT 
GTGATCACTG GCCGAGGTCA CGGCGGCAAG ATCAAGTCAG GCCAGATCTG GATCAATGGG 
CAGCCCAGCT CGCCTCAGCT GGTGAGGAAG TGTGTGGCCC ACGTGCGCCA GCACAACCAG 
CTGCTCCCCA ACTTGACTGT GCGAGAGACC TTGGCCTTCA TTGCCCAGAT GCGGCTGCCC 
AGAACCTTCT CCCAGGCCCA GCGTGACAAA AGGGTGGAGG ACGTGATCGC GGAGCTGCGG 
CTTAGGCAGT GCGCTGACAC CCGCGTGGGC AACATGTACG TGCGGGGGTT GTCGGGGGGT 
GAGCGCAGGA GAGTCAGCAT TGGGGTGCAG CTCCTGTGGA ACCCAGGAAT CCTTATTCTC 
GACGAACCCA CCTCTGGGCT CGACAGCTTC ACAGCCCACA ACCTGGTGAA GACCTTGTCC 
AGGCTGGCCA AAGGCAACCG GCTGGTGCTC ATCTCCCTCC ACCAGCCTCG CTCTGACATC 
TTCAGGCTGT TTGATCTGGT CCTCCTGATG ACGTCTGGCA CCCCCATCTA CTTAGGGGCG 
GCCCAGCACA TGGTCCAGTA TTTCACAGCC ATCGGCTACC CCTGTCCTCG CTACAGCAAT 
CCTGCTGACT TCTATGTGGA CCTGACCAGC ATTGACAGGC GCAGCAGAGA GCAGGAATTG 
GCCACCAGGG AGAAGGCTCA GTCACTCGCA GCCCTGTTTC TAGAAAAAGT GCGTGACTTA 
GATGACTTTC TATGGAAAGC AGAGACGAAG GATCTTGACG AGGACACCTG TGTGGAAAGC 
GTGACCCCAC TAGACACCAA CTGCCTCCCG AGTCCTACGA AGATGCCTGG GGCGGTGCAG 
CAGTTTACGA CGCTGATCCG TCGTCAGATT TCCAACGACT TCCGAGACCT GCCCACCCTC 
CTCATCCATG GGGCGGAGGC CTGTCTGATG TCAATGACCA TCGGCTTCCT CTATTTTGGC 
CATGGGAGCA TCCAGCTCTC CTTCATGGAT ACAGCCGCCC TCTTGTTCAT GATCGGTGCT 
CTCATCCCTT TCAACGTCAT TCTGGATGTC ATCTCCAAAT GTTACTCAGA GAGGGCAATG 
CTTTACTATG AACTGGAAGA CGGGCTGTAC ACCACTGGTC CATATTTCTT TGCCAAGATC 
CTCGGGGAGC TTCCGGAGCA CTGTGCCTAC ATCATCATCT ACGGGATGCC CACCTACTGG 
CTGGCCAACC TGAGGCCAGG CCTCCAGCCC TTCCTGCTGC ACTTCCTGCT GGTGTGGCTG 
GTGGTCTTCT GTTGCAGGAT TATGGCCCTG GCCGCCGCGG CCCTGCTCCC CACCTTCCAC 
ATGGCCTCCT TCTTCAGCAA TGCCCTCTAC AACTCCTTCT ACCTCGCCGG GGGCTTCATG 
ATAAACTTGA GCAGCCTGTG GACAGTGCCC GCGTGGATTT CCAAAGTGTC CTTCCTGCGG 
TGGTGTTTTG AAGGGCTGAT GAAGATTCAG TTCAGCAGAA GAACTTATAA AATGCCTCTC 
GGGAACCTCA CCATCGCGGT CTCAGGAGAT AAAATCCTCA GTGTCATGGA GCTGGACTCG 
TACCCTCTCT ACGCCATCTA CCTCATCGTC ATTGGCCTCA GCGGTGGCTT CATGGTCCTG 
TACTACGTGT CCTTAAGGTT CATCAAACAG AAACCAAGTC AAGACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531332.1
CDS	491..2521
Translation	MQSLKPHPRPAESALLQSSLRSTGLGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW

Target ORF information:

RefSeq Version	XM_011533030.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP binding cassette subfamily G member 8 (ABCG8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533030.2

ORF Insert Sequence:

ATGCAGAGCC TCAAGCCGCA CCCAAGACCT GCTGAGTCAG CTCTGCTTCA GTCGAGTTTG 
AGAAGCACTG GCTTGGGCCT CCAGGATAGA TTGTTCTCCT CTGAAAGTGA CAACAGCCTG 
TACTTCACCT ACAGTGGCCA GCCCAACACC CTGGAGGTCA GAGACCTCAA CTACCAGGTG 
GACCTGGCCT CTCAGGTCCC TTGGTTTGAG CAGCTGGCTC AGTTCAAGAT GCCCTGGACA 
TCTCCCAGCT GCCAGAATTC TTGTGAGCTG GGCATCCAGA ACCTAAGCTT CAAAGTGAGA 
AGTGGGCAGA TGCTGGCCAT CATAGGGAGC TCAGGTTGTG GGAGAGCCTC CTTGCTAGAT 
GTGATCACTG GCCGAGGTCA CGGCGGCAAG ATCAAGTCAG GCCAGATCTG GATCAATGGG 
CAGCCCAGCT CGCCTCAGCT GGTGAGGAAG TGTGTGGCCC ACGTGCGCCA GCACAACCAG 
CTGCTCCCCA ACTTGACTGT GCGAGAGACC TTGGCCTTCA TTGCCCAGAT GCGGCTGCCC 
AGAACCTTCT CCCAGGCCCA GCGTGACAAA AGGGTGGAGG ACGTGATCGC GGAGCTGCGG 
CTTAGGCAGT GCGCTGACAC CCGCGTGGGC AACATGTACG TGCGGGGGTT GTCGGGGGGT 
GAGCGCAGGA GAGTCAGCAT TGGGGTGCAG CTCCTGTGGA ACCCAGGAAT CCTTATTCTC 
GACGAACCCA CCTCTGGGCT CGACAGCTTC ACAGCCCACA ACCTGGTGAA GACCTTGTCC 
AGGCTGGCCA AAGGCAACCG GCTGGTGCTC ATCTCCCTCC ACCAGCCTCG CTCTGACATC 
TTCAGGCTGT TTGATCTGGT CCTCCTGATG ACGTCTGGCA CCCCCATCTA CTTAGGGGCG 
GCCCAGCACA TGGTCCAGTA TTTCACAGCC ATCGGCTACC CCTGTCCTCG CTACAGCAAT 
CCTGCTGACT TCTATGTGGA CCTGACCAGC ATTGACAGGC GCAGCAGAGA GCAGGAATTG 
GCCACCAGGG AGAAGGCTCA GTCACTCGCA GCCCTGTTTC TAGAAAAAGT GCGTGACTTA 
GATGACTTTC TATGGAAAGC AGAGACGAAG GATCTTGACG AGGACACCTG TGTGGAAAGC 
GTGACCCCAC TAGACACCAA CTGCCTCCCG AGTCCTACGA AGATGCCTGG GGCGGTGCAG 
CAGTTTACGA CGCTGATCCG TCGTCAGATT TCCAACGACT TCCGAGACCT GCCCACCCTC 
CTCATCCATG GGGCGGAGGC CTGTCTGATG TCAATGACCA TCGGCTTCCT CTATTTTGGC 
CATGGGAGCA TCCAGCTCTC CTTCATGGAT ACAGCCGCCC TCTTGTTCAT GATCGGTGCT 
CTCATCCCTT TCAACGTCAT TCTGGATGTC ATCTCCAAAT GTTACTCAGA GAGGGCAATG 
CTTTACTATG AACTGGAAGA CGGGCTGTAC ACCACTGGTC CATATTTCTT TGCCAAGATC 
CTCGGGGAGC TTCCGGAGCA CTGTGCCTAC ATCATCATCT ACGGGATGCC CACCTACTGG 
CTGGCCAACC TGAGGCCAGG CCTCCAGCCC TTCCTGCTGC ACTTCCTGCT GGTGTGGCTG 
GTGGTCTTCT GTTGCAGGAT TATGGCCCTG GCCGCCGCGG CCCTGCTCCC CACCTTCCAC 
ATGGCCTCCT TCTTCAGCAA TGCCCTCTAC AACTCCTTCT ACCTCGCCGG GGGCTTCATG 
ATAAACTTGA GCAGCCTGTG GACAGTGCCC GCGTGGATTT CCAAAGTGTC CTTCCTGCGG 
TGGTGTTTTG AAGGGCTGAT GAAGATTCAG TTCAGCAGAA GAACTTATAA AATGCCTCTC 
GGGAACCTCA CCATCGCGGT CTCAGGAGAT AAAATCCTCA GTGTCATGGA GCTGGACTCG 
TACCCTCTCT ACGCCATCTA CCTCATCGTC ATTGGCCTCA GCGGTGGCTT CATGGTCCTG 
TACTACGTGT CCTTAAGGTT CATCAAACAG AAACCAAGTC AAGACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in the ABCG8 gene are associated with sitosterolaemia in the homozygous form and xanthelasmas in the heterozygous form.
European journal of dermatology : EJD27(5)519-523(2017 Oct)
Bardawil T,Rebeiz A,Chaabouni M,El Halabi J,Kambris Z,Abbas O,Abou Hassan O,Hamie L,Bitar F,Ghani Kibbi A,Nemer G,Kurban M

Timely diagnosis of sitosterolemia by next generation sequencing in two children with severe hypercholesterolemia.
Atherosclerosis26271-77(2017 Jul)
Buonuomo PS,Iughetti L,Pisciotta L,Rabacchi C,Papadia F,Bruzzi P,Tummolo A,Bartuli A,Cortese C,Bertolini S,Calandra S

Cholelithiasis in Patients with Gaucher Disease type 1: Risk Factors and the Role of ABCG5/ABCG8 Gene Variants.
Journal of gastrointestinal and liver diseases : JGLD25(4)447-455(2016 12)
Zimmermann A,Popp RA,Al-Khzouz C,Bucerzan S,Na?cu I,Leucuta D,Galle PR,Grigorescu-Sido P

Crystal structure of the human sterol transporter ABCG5/ABCG8.
Nature533(7604)561-4(2016 05)
Lee JY,Kinch LN,Borek DM,Wang J,Wang J,Urbatsch IL,Xie XS,Grishin NV,Cohen JC,Otwinowski Z,Hobbs HH,Rosenbaum DM

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ABCG8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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