gRNAs
The following Abcg8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Abcg8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu16792 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001286005.1 | |
| Accession Version | NM_001286005.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Mus musculus(house mouse) | |
| Product | ATP-binding cassette sub-family G member 8 isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC165360.3 and AF324495.1. Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK050938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849389 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAAATCAG GACAAATTTG GATAAATGGG CAACCCAGTA CGCCTCAGCT GGTGAGGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001272934.1 |
| CDS | 241..1881 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001286005.1 |
1 | ATGAAATCAG GACAAATTTG GATAAATGGG CAACCCAGTA CGCCTCAGCT GGTGAGGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu18401 | |
| Clone ID Related Accession (Same CDS sequence) | NM_026180.3 | |
| Accession Version | NM_026180.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-23 | |
| Organism | Mus musculus(house mouse) | |
| Product | ATP-binding cassette sub-family G member 8 isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC165360.3, AK004871.1 and AF324495.1. On Oct 26, 2013 this sequence version replaced NM_026180.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF324495.1, AK004871.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCTGAGA AAACCAAAGA AGAGACCCAG CTGTGGAATG GGACTGTACT TCAGGATGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_080456.1 |
| CDS | 112..2133 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_026180.3 |
1 | ATGGCTGAGA AAACCAAAGA AGAGACCCAG CTGTGGAATG GGACTGTACT TCAGGATGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu53953 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001347418.1 | |
| Accession Version | NM_001347418.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-28 | |
| Organism | Mus musculus(house mouse) | |
| Product | ATP-binding cassette sub-family G member 8 isoform 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC165360.3 and AF324495.1. On Nov 26, 2016 this sequence version replaced XM_006524826.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY196216.1, AY196215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCTGAGA AAACCAAAGA AGAGACCCAG CTGTGGAATG GGACTGTACT TCAGGATGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001334347.1 |
| CDS | 112..2130 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001347418.1 |
1 | ATGGCTGAGA AAACCAAAGA AGAGACCCAG CTGTGGAATG GGACTGTACT TCAGGATGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Intestinal Farnesoid X Receptor Controls Transintestinal Cholesterol Excretion in Mice. |
Inhibition of Ceramide Decreased the Expression of ATP-Binding Cassette Transporter G5/8 mRNA in an Animal Model of Cholesterol Gallstone. |
Evidence that the adenosine triphosphate-binding cassette G5/G8-independent pathway plays a determinant role in cholesterol gallstone formation in mice. |
Hepatic ABCG5/G8 overexpression substantially increases biliary cholesterol secretion but does not impact in?vivo macrophage-to-feces RCT. |
