PJA1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_145119.3	NP_660095.1	E3 ubiquitin-protein ligase Praja-1 isoform a
NM_022368.5	NP_071763.2	E3 ubiquitin-protein ligase Praja-1 isoform c
XM_011531011.2	XP_011529313.1	E3 ubiquitin-protein ligase Praja-1 isoform X1
XM_005262292.1	XP_005262349.1	E3 ubiquitin-protein ligase Praja-1 isoform X2
XM_011531011.1	XP_011529313.1	E3 ubiquitin-protein ligase Praja-1 isoform X2
NM_001032396.2	NP_001027568.1	E3 ubiquitin-protein ligase Praja-1 isoform b
NM_001032396.3	NP_001027568.1	E3 ubiquitin-protein ligase Praja-1 isoform b
NM_145119.4	NP_660095.1	E3 ubiquitin-protein ligase Praja-1 isoform a
NM_022368.4	NP_071763.2	E3 ubiquitin-protein ligase Praja-1 isoform c

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04538	NM_001032396.3 Latest version!	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu55728	NM_145119.4 Latest version!	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu04538	XM_005262292.1 Latest version!	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu55728	XM_011531011.2 Latest version!	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20845	NM_022368.5 Latest version!	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu55728	XM_011531011.1	Homo sapiens praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu04538	NM_001032396.2	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu55728	NM_145119.3	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20845	NM_022368.4	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

PJA1 ( NM_001032396.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001027568.1 Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04538
Clone ID Related Accession (Same CDS sequence)	XM_005262292.1 , NM_001032396.2 , NM_001032396.3
Accession Version	NM_001032396.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC349747.1, AK226171.1 and BM127146.1. On May 15, 2010 this sequence version replaced NM_001032396.1. Transcript Variant: This variant (2, also known as PJA1beta) lacks a portion of the 5' UTR and 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.157609.1, SRR1660803.141320.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2147920 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCGCCATT TTCCACTACT 
CGTCGTAGTT GGGACGACAG CGAGAGTTCG GGAACCAACC TGAATATTGA TAATGAGGAC 
TATTCCAGGT ATCCGCCAAG AGAGTACAGA GCTTCGGGTA GCAGAAGAGG AATGGCCTAT 
GGACATATTG ACTCTTATGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG 
CGACCGCCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCAGAG 
AAAGGGGCAA GGTCTTTGGC TGGGCCACCT CCACATTTCT CTAGTTTTAG CCGTGATGTG 
AGAGAGGAGC GAGACAAGTT AGACCCAGTC CCTGCAGCAA GATGCTCAGC TAGCAGAGCT 
GACTTCCTGC CACAAAGTAG TGTGGCCTCA CAGTCGTCTT CTGAAGGCAA GCTGGCTACA 
AAAGGTGACA GCTCGGAGAG GGAGAGAAGG GAGCAAAATT TACCTGCACG TCCCAGCAGG 
GCTCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCTCAA AGAGTGCAGA GGAACCTGTG 
GTCAGGCCCA AAATCAGAAA CCTGGCAAGT CCAAACTGCG TGAAACCAAA AATTTTTTTT 
GATACTGATG ATGATGACGA TATGCCACAC AGTACTTCCA GGTGGAGGGA TACCGCCAAT 
GACAATGAGG GCCACTCGGA TGGCCTGGCA AGAAGAGGGA GAGGCGAGAG TTCAAGTGGC 
TATCCCGAGC CAAAGTACCC TGAAGACAAA CGGGAAGCGA GGAGTGACCA AGTGAAACCA 
GAAAAGGTGC CGAGACGACG ACGCACCATG GCCGACCCTG ACTTCTGGAC GCACAGTGAT 
GATTACTACA AATACTGCGA CGAAGACTCT GACAGTGACA AAGAGTGGAT TGCTGCTCTG 
CGTCGGAAAT ATCGAAGCCG AGAGCAAACC CTGTCCTCCA GTGGCGAAAG CTGGGAGACT 
CTGCCGGGGA AAGAAGAGCG GGAACCTCCA CAGGCTAAGG TGAGTGCCAG CACTGGCACC 
AGCCCTGGCC CCGGTGCTAG TGCCAGTGCC GGGGCTGGCG CCGGGGCCAG TGCTGGCAGC 
AATGGCAGCA ATTACCTTGA AGAAGTTCGA GAACCATCTC TTCAGGAAGA GCAGGCATCC 
CTGGAAGAAG GAGAAATTCC TTGGCTCCAG TACCATGAGA ATGACAGTAG CAGTGAGGGG 
GATAATGATT CTGGTCACGA GTTGATGCAA CCTGGGGTAT TCATGCTGGA TGGAAACAAC 
AACCTTGAAG ATGACTCCAG TGTGAGCGAA GACCTAGAAG TGGATTGGAG CCTCTTTGAT 
GGATTTGCAG ATGGGTTAGG AGTGGCTGAA GCCATTTCCT ATGTGGACCC TCAGTTCCTC 
ACCTACATGG CACTTGAAGA ACGCCTGGCC CAGGCAATGG AAACTGCCCT TGCGCACTTG 
GAGTCTCTCG CAGTGGATGT AGAGGTGGCC AATCCACCAG CAAGCAAGGA GAGCATTGAC 
GCTCTTCCCG AGATCCTGGT CACTGAAGAT CATGGCGCAG TTGGTCAGGA GATGTGCTGC 
CCCATCTGCT GTAGCGAATA TGTGAAGGGG GAGGTGGCAA CTGAGCTGCC GTGCCACCAC 
TATTTCCACA AGCCGTGTGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC 
CGCTGCATGT TCCCTCCCCC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027568.1
CDS	219..1985
Translation	MHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Target ORF information:

RefSeq Version	NM_001032396.2
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032396.2

ORF Insert Sequence:

ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCGCCATT TTCCACTACT 
CGTCGTAGTT GGGACGACAG CGAGAGTTCG GGAACCAACC TGAATATTGA TAATGAGGAC 
TATTCCAGGT ATCCGCCAAG AGAGTACAGA GCTTCGGGTA GCAGAAGAGG AATGGCCTAT 
GGACATATTG ACTCTTATGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG 
CGACCGCCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCAGAG 
AAAGGGGCAA GGTCTTTGGC TGGGCCACCT CCACATTTCT CTAGTTTTAG CCGTGATGTG 
AGAGAGGAGC GAGACAAGTT AGACCCAGTC CCTGCAGCAA GATGCTCAGC TAGCAGAGCT 
GACTTCCTGC CACAAAGTAG TGTGGCCTCA CAGTCGTCTT CTGAAGGCAA GCTGGCTACA 
AAAGGTGACA GCTCGGAGAG GGAGAGAAGG GAGCAAAATT TACCTGCACG TCCCAGCAGG 
GCTCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCTCAA AGAGTGCAGA GGAACCTGTG 
GTCAGGCCCA AAATCAGAAA CCTGGCAAGT CCAAACTGCG TGAAACCAAA AATTTTTTTT 
GATACTGATG ATGATGACGA TATGCCACAC AGTACTTCCA GGTGGAGGGA TACCGCCAAT 
GACAATGAGG GCCACTCGGA TGGCCTGGCA AGAAGAGGGA GAGGCGAGAG TTCAAGTGGC 
TATCCCGAGC CAAAGTACCC TGAAGACAAA CGGGAAGCGA GGAGTGACCA AGTGAAACCA 
GAAAAGGTGC CGAGACGACG ACGCACCATG GCCGACCCTG ACTTCTGGAC GCACAGTGAT 
GATTACTACA AATACTGCGA CGAAGACTCT GACAGTGACA AAGAGTGGAT TGCTGCTCTG 
CGTCGGAAAT ATCGAAGCCG AGAGCAAACC CTGTCCTCCA GTGGCGAAAG CTGGGAGACT 
CTGCCGGGGA AAGAAGAGCG GGAACCTCCA CAGGCTAAGG TGAGTGCCAG CACTGGCACC 
AGCCCTGGCC CCGGTGCTAG TGCCAGTGCC GGGGCTGGCG CCGGGGCCAG TGCTGGCAGC 
AATGGCAGCA ATTACCTTGA AGAAGTTCGA GAACCATCTC TTCAGGAAGA GCAGGCATCC 
CTGGAAGAAG GAGAAATTCC TTGGCTCCAG TACCATGAGA ATGACAGTAG CAGTGAGGGG 
GATAATGATT CTGGTCACGA GTTGATGCAA CCTGGGGTAT TCATGCTGGA TGGAAACAAC 
AACCTTGAAG ATGACTCCAG TGTGAGCGAA GACCTAGAAG TGGATTGGAG CCTCTTTGAT 
GGATTTGCAG ATGGGTTAGG AGTGGCTGAA GCCATTTCCT ATGTGGACCC TCAGTTCCTC 
ACCTACATGG CACTTGAAGA ACGCCTGGCC CAGGCAATGG AAACTGCCCT TGCGCACTTG 
GAGTCTCTCG CAGTGGATGT AGAGGTGGCC AATCCACCAG CAAGCAAGGA GAGCATTGAC 
GCTCTTCCCG AGATCCTGGT CACTGAAGAT CATGGCGCAG TTGGTCAGGA GATGTGCTGC 
CCCATCTGCT GTAGCGAATA TGTGAAGGGG GAGGTGGCAA CTGAGCTGCC GTGCCACCAC 
TATTTCCACA AGCCGTGTGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC 
CGCTGCATGT TCCCTCCCCC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA1 ( NM_001032396.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001027568.1 Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04538
Clone ID Related Accession (Same CDS sequence)	XM_005262292.1 , NM_001032396.2 , NM_001032396.3
Accession Version	NM_001032396.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC349747.1, AK226171.1 and BM127146.1. On Jun 2, 2019 this sequence version replaced NM_001032396.2. Transcript Variant: This variant (2, also known as PJA1beta) lacks a portion of the 5' UTR and 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.89691.1, SRR1803615.155938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2147920 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCGCCATT TTCCACTACT 
CGTCGTAGTT GGGACGACAG CGAGAGTTCG GGAACCAACC TGAATATTGA TAATGAGGAC 
TATTCCAGGT ATCCGCCAAG AGAGTACAGA GCTTCGGGTA GCAGAAGAGG AATGGCCTAT 
GGACATATTG ACTCTTATGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG 
CGACCGCCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCAGAG 
AAAGGGGCAA GGTCTTTGGC TGGGCCACCT CCACATTTCT CTAGTTTTAG CCGTGATGTG 
AGAGAGGAGC GAGACAAGTT AGACCCAGTC CCTGCAGCAA GATGCTCAGC TAGCAGAGCT 
GACTTCCTGC CACAAAGTAG TGTGGCCTCA CAGTCGTCTT CTGAAGGCAA GCTGGCTACA 
AAAGGTGACA GCTCGGAGAG GGAGAGAAGG GAGCAAAATT TACCTGCACG TCCCAGCAGG 
GCTCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCTCAA AGAGTGCAGA GGAACCTGTG 
GTCAGGCCCA AAATCAGAAA CCTGGCAAGT CCAAACTGCG TGAAACCAAA AATTTTTTTT 
GATACTGATG ATGATGACGA TATGCCACAC AGTACTTCCA GGTGGAGGGA TACCGCCAAT 
GACAATGAGG GCCACTCGGA TGGCCTGGCA AGAAGAGGGA GAGGCGAGAG TTCAAGTGGC 
TATCCCGAGC CAAAGTACCC TGAAGACAAA CGGGAAGCGA GGAGTGACCA AGTGAAACCA 
GAAAAGGTGC CGAGACGACG ACGCACCATG GCCGACCCTG ACTTCTGGAC GCACAGTGAT 
GATTACTACA AATACTGCGA CGAAGACTCT GACAGTGACA AAGAGTGGAT TGCTGCTCTG 
CGTCGGAAAT ATCGAAGCCG AGAGCAAACC CTGTCCTCCA GTGGCGAAAG CTGGGAGACT 
CTGCCGGGGA AAGAAGAGCG GGAACCTCCA CAGGCTAAGG TGAGTGCCAG CACTGGCACC 
AGCCCTGGCC CCGGTGCTAG TGCCAGTGCC GGGGCTGGCG CCGGGGCCAG TGCTGGCAGC 
AATGGCAGCA ATTACCTTGA AGAAGTTCGA GAACCATCTC TTCAGGAAGA GCAGGCATCC 
CTGGAAGAAG GAGAAATTCC TTGGCTCCAG TACCATGAGA ATGACAGTAG CAGTGAGGGG 
GATAATGATT CTGGTCACGA GTTGATGCAA CCTGGGGTAT TCATGCTGGA TGGAAACAAC 
AACCTTGAAG ATGACTCCAG TGTGAGCGAA GACCTAGAAG TGGATTGGAG CCTCTTTGAT 
GGATTTGCAG ATGGGTTAGG AGTGGCTGAA GCCATTTCCT ATGTGGACCC TCAGTTCCTC 
ACCTACATGG CACTTGAAGA ACGCCTGGCC CAGGCAATGG AAACTGCCCT TGCGCACTTG 
GAGTCTCTCG CAGTGGATGT AGAGGTGGCC AATCCACCAG CAAGCAAGGA GAGCATTGAC 
GCTCTTCCCG AGATCCTGGT CACTGAAGAT CATGGCGCAG TTGGTCAGGA GATGTGCTGC 
CCCATCTGCT GTAGCGAATA TGTGAAGGGG GAGGTGGCAA CTGAGCTGCC GTGCCACCAC 
TATTTCCACA AGCCGTGTGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC 
CGCTGCATGT TCCCTCCCCC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027568.1
CDS	150..1916
Translation	MHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Target ORF information:

RefSeq Version	NM_001032396.3
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032396.3

ORF Insert Sequence:

ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCGCCATT TTCCACTACT 
CGTCGTAGTT GGGACGACAG CGAGAGTTCG GGAACCAACC TGAATATTGA TAATGAGGAC 
TATTCCAGGT ATCCGCCAAG AGAGTACAGA GCTTCGGGTA GCAGAAGAGG AATGGCCTAT 
GGACATATTG ACTCTTATGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG 
CGACCGCCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCAGAG 
AAAGGGGCAA GGTCTTTGGC TGGGCCACCT CCACATTTCT CTAGTTTTAG CCGTGATGTG 
AGAGAGGAGC GAGACAAGTT AGACCCAGTC CCTGCAGCAA GATGCTCAGC TAGCAGAGCT 
GACTTCCTGC CACAAAGTAG TGTGGCCTCA CAGTCGTCTT CTGAAGGCAA GCTGGCTACA 
AAAGGTGACA GCTCGGAGAG GGAGAGAAGG GAGCAAAATT TACCTGCACG TCCCAGCAGG 
GCTCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCTCAA AGAGTGCAGA GGAACCTGTG 
GTCAGGCCCA AAATCAGAAA CCTGGCAAGT CCAAACTGCG TGAAACCAAA AATTTTTTTT 
GATACTGATG ATGATGACGA TATGCCACAC AGTACTTCCA GGTGGAGGGA TACCGCCAAT 
GACAATGAGG GCCACTCGGA TGGCCTGGCA AGAAGAGGGA GAGGCGAGAG TTCAAGTGGC 
TATCCCGAGC CAAAGTACCC TGAAGACAAA CGGGAAGCGA GGAGTGACCA AGTGAAACCA 
GAAAAGGTGC CGAGACGACG ACGCACCATG GCCGACCCTG ACTTCTGGAC GCACAGTGAT 
GATTACTACA AATACTGCGA CGAAGACTCT GACAGTGACA AAGAGTGGAT TGCTGCTCTG 
CGTCGGAAAT ATCGAAGCCG AGAGCAAACC CTGTCCTCCA GTGGCGAAAG CTGGGAGACT 
CTGCCGGGGA AAGAAGAGCG GGAACCTCCA CAGGCTAAGG TGAGTGCCAG CACTGGCACC 
AGCCCTGGCC CCGGTGCTAG TGCCAGTGCC GGGGCTGGCG CCGGGGCCAG TGCTGGCAGC 
AATGGCAGCA ATTACCTTGA AGAAGTTCGA GAACCATCTC TTCAGGAAGA GCAGGCATCC 
CTGGAAGAAG GAGAAATTCC TTGGCTCCAG TACCATGAGA ATGACAGTAG CAGTGAGGGG 
GATAATGATT CTGGTCACGA GTTGATGCAA CCTGGGGTAT TCATGCTGGA TGGAAACAAC 
AACCTTGAAG ATGACTCCAG TGTGAGCGAA GACCTAGAAG TGGATTGGAG CCTCTTTGAT 
GGATTTGCAG ATGGGTTAGG AGTGGCTGAA GCCATTTCCT ATGTGGACCC TCAGTTCCTC 
ACCTACATGG CACTTGAAGA ACGCCTGGCC CAGGCAATGG AAACTGCCCT TGCGCACTTG 
GAGTCTCTCG CAGTGGATGT AGAGGTGGCC AATCCACCAG CAAGCAAGGA GAGCATTGAC 
GCTCTTCCCG AGATCCTGGT CACTGAAGAT CATGGCGCAG TTGGTCAGGA GATGTGCTGC 
CCCATCTGCT GTAGCGAATA TGTGAAGGGG GAGGTGGCAA CTGAGCTGCC GTGCCACCAC 
TATTTCCACA AGCCGTGTGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC 
CGCTGCATGT TCCCTCCCCC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA1 ( NM_145119.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_660095.1 Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55728
Clone ID Related Accession (Same CDS sequence)	NM_145119.3 , XM_011531011.2 , XM_011531011.1 , NM_145119.4
Accession Version	NM_145119.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC349747.1, BC075803.1 and BM127146.1. On May 15, 2010 this sequence version replaced NM_145119.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK096611.1, SRR1803615.6526.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGGTATCCG 
CCAAGAGAGT ACAGAGCTTC GGGTAGCAGA AGAGGAATGG CCTATGGACA TATTGACTCT 
TATGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGACC GCCAGTGAGA 
GGGAAAACTG GCAAGTTTAA AGATGATAAG CTGTATGACC CAGAGAAAGG GGCAAGGTCT 
TTGGCTGGGC CACCTCCACA TTTCTCTAGT TTTAGCCGTG ATGTGAGAGA GGAGCGAGAC 
AAGTTAGACC CAGTCCCTGC AGCAAGATGC TCAGCTAGCA GAGCTGACTT CCTGCCACAA 
AGTAGTGTGG CCTCACAGTC GTCTTCTGAA GGCAAGCTGG CTACAAAAGG TGACAGCTCG 
GAGAGGGAGA GAAGGGAGCA AAATTTACCT GCACGTCCCA GCAGGGCTCC TGTGAGTATT 
TGTGGTGGTG GGGAAAACAC CTCAAAGAGT GCAGAGGAAC CTGTGGTCAG GCCCAAAATC 
AGAAACCTGG CAAGTCCAAA CTGCGTGAAA CCAAAAATTT TTTTTGATAC TGATGATGAT 
GACGATATGC CACACAGTAC TTCCAGGTGG AGGGATACCG CCAATGACAA TGAGGGCCAC 
TCGGATGGCC TGGCAAGAAG AGGGAGAGGC GAGAGTTCAA GTGGCTATCC CGAGCCAAAG 
TACCCTGAAG ACAAACGGGA AGCGAGGAGT GACCAAGTGA AACCAGAAAA GGTGCCGAGA 
CGACGACGCA CCATGGCCGA CCCTGACTTC TGGACGCACA GTGATGATTA CTACAAATAC 
TGCGACGAAG ACTCTGACAG TGACAAAGAG TGGATTGCTG CTCTGCGTCG GAAATATCGA 
AGCCGAGAGC AAACCCTGTC CTCCAGTGGC GAAAGCTGGG AGACTCTGCC GGGGAAAGAA 
GAGCGGGAAC CTCCACAGGC TAAGGTGAGT GCCAGCACTG GCACCAGCCC TGGCCCCGGT 
GCTAGTGCCA GTGCCGGGGC TGGCGCCGGG GCCAGTGCTG GCAGCAATGG CAGCAATTAC 
CTTGAAGAAG TTCGAGAACC ATCTCTTCAG GAAGAGCAGG CATCCCTGGA AGAAGGAGAA 
ATTCCTTGGC TCCAGTACCA TGAGAATGAC AGTAGCAGTG AGGGGGATAA TGATTCTGGT 
CACGAGTTGA TGCAACCTGG GGTATTCATG CTGGATGGAA ACAACAACCT TGAAGATGAC 
TCCAGTGTGA GCGAAGACCT AGAAGTGGAT TGGAGCCTCT TTGATGGATT TGCAGATGGG 
TTAGGAGTGG CTGAAGCCAT TTCCTATGTG GACCCTCAGT TCCTCACCTA CATGGCACTT 
GAAGAACGCC TGGCCCAGGC AATGGAAACT GCCCTTGCGC ACTTGGAGTC TCTCGCAGTG 
GATGTAGAGG TGGCCAATCC ACCAGCAAGC AAGGAGAGCA TTGACGCTCT TCCCGAGATC 
CTGGTCACTG AAGATCATGG CGCAGTTGGT CAGGAGATGT GCTGCCCCAT CTGCTGTAGC 
GAATATGTGA AGGGGGAGGT GGCAACTGAG CTGCCGTGCC ACCACTATTT CCACAAGCCG 
TGTGTGTCCA TCTGGCTTCA GAAGTCAGGC ACCTGCCCCG TGTGCCGCTG CATGTTCCCT 
CCCCCACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660095.1
CDS	380..2311
Translation	MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERIASQRKTNSEVPMHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Target ORF information:

RefSeq Version	NM_145119.3
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145119.3

ORF Insert Sequence:

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGGTATCCG 
CCAAGAGAGT ACAGAGCTTC GGGTAGCAGA AGAGGAATGG CCTATGGACA TATTGACTCT 
TATGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGACC GCCAGTGAGA 
GGGAAAACTG GCAAGTTTAA AGATGATAAG CTGTATGACC CAGAGAAAGG GGCAAGGTCT 
TTGGCTGGGC CACCTCCACA TTTCTCTAGT TTTAGCCGTG ATGTGAGAGA GGAGCGAGAC 
AAGTTAGACC CAGTCCCTGC AGCAAGATGC TCAGCTAGCA GAGCTGACTT CCTGCCACAA 
AGTAGTGTGG CCTCACAGTC GTCTTCTGAA GGCAAGCTGG CTACAAAAGG TGACAGCTCG 
GAGAGGGAGA GAAGGGAGCA AAATTTACCT GCACGTCCCA GCAGGGCTCC TGTGAGTATT 
TGTGGTGGTG GGGAAAACAC CTCAAAGAGT GCAGAGGAAC CTGTGGTCAG GCCCAAAATC 
AGAAACCTGG CAAGTCCAAA CTGCGTGAAA CCAAAAATTT TTTTTGATAC TGATGATGAT 
GACGATATGC CACACAGTAC TTCCAGGTGG AGGGATACCG CCAATGACAA TGAGGGCCAC 
TCGGATGGCC TGGCAAGAAG AGGGAGAGGC GAGAGTTCAA GTGGCTATCC CGAGCCAAAG 
TACCCTGAAG ACAAACGGGA AGCGAGGAGT GACCAAGTGA AACCAGAAAA GGTGCCGAGA 
CGACGACGCA CCATGGCCGA CCCTGACTTC TGGACGCACA GTGATGATTA CTACAAATAC 
TGCGACGAAG ACTCTGACAG TGACAAAGAG TGGATTGCTG CTCTGCGTCG GAAATATCGA 
AGCCGAGAGC AAACCCTGTC CTCCAGTGGC GAAAGCTGGG AGACTCTGCC GGGGAAAGAA 
GAGCGGGAAC CTCCACAGGC TAAGGTGAGT GCCAGCACTG GCACCAGCCC TGGCCCCGGT 
GCTAGTGCCA GTGCCGGGGC TGGCGCCGGG GCCAGTGCTG GCAGCAATGG CAGCAATTAC 
CTTGAAGAAG TTCGAGAACC ATCTCTTCAG GAAGAGCAGG CATCCCTGGA AGAAGGAGAA 
ATTCCTTGGC TCCAGTACCA TGAGAATGAC AGTAGCAGTG AGGGGGATAA TGATTCTGGT 
CACGAGTTGA TGCAACCTGG GGTATTCATG CTGGATGGAA ACAACAACCT TGAAGATGAC 
TCCAGTGTGA GCGAAGACCT AGAAGTGGAT TGGAGCCTCT TTGATGGATT TGCAGATGGG 
TTAGGAGTGG CTGAAGCCAT TTCCTATGTG GACCCTCAGT TCCTCACCTA CATGGCACTT 
GAAGAACGCC TGGCCCAGGC AATGGAAACT GCCCTTGCGC ACTTGGAGTC TCTCGCAGTG 
GATGTAGAGG TGGCCAATCC ACCAGCAAGC AAGGAGAGCA TTGACGCTCT TCCCGAGATC 
CTGGTCACTG AAGATCATGG CGCAGTTGGT CAGGAGATGT GCTGCCCCAT CTGCTGTAGC 
GAATATGTGA AGGGGGAGGT GGCAACTGAG CTGCCGTGCC ACCACTATTT CCACAAGCCG 
TGTGTGTCCA TCTGGCTTCA GAAGTCAGGC ACCTGCCCCG TGTGCCGCTG CATGTTCCCT 
CCCCCACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA1 ( NM_145119.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_660095.1 Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 1, mRNA.

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CloneID	OHu55728
Clone ID Related Accession (Same CDS sequence)	NM_145119.3 , XM_011531011.2 , XM_011531011.1 , NM_145119.4
Accession Version	NM_145119.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC349747.1, BC075803.1 and BM127146.1. On Jun 2, 2019 this sequence version replaced NM_145119.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.249082.1, SRR1803616.114827.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGGTATCCG 
CCAAGAGAGT ACAGAGCTTC GGGTAGCAGA AGAGGAATGG CCTATGGACA TATTGACTCT 
TATGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGACC GCCAGTGAGA 
GGGAAAACTG GCAAGTTTAA AGATGATAAG CTGTATGACC CAGAGAAAGG GGCAAGGTCT 
TTGGCTGGGC CACCTCCACA TTTCTCTAGT TTTAGCCGTG ATGTGAGAGA GGAGCGAGAC 
AAGTTAGACC CAGTCCCTGC AGCAAGATGC TCAGCTAGCA GAGCTGACTT CCTGCCACAA 
AGTAGTGTGG CCTCACAGTC GTCTTCTGAA GGCAAGCTGG CTACAAAAGG TGACAGCTCG 
GAGAGGGAGA GAAGGGAGCA AAATTTACCT GCACGTCCCA GCAGGGCTCC TGTGAGTATT 
TGTGGTGGTG GGGAAAACAC CTCAAAGAGT GCAGAGGAAC CTGTGGTCAG GCCCAAAATC 
AGAAACCTGG CAAGTCCAAA CTGCGTGAAA CCAAAAATTT TTTTTGATAC TGATGATGAT 
GACGATATGC CACACAGTAC TTCCAGGTGG AGGGATACCG CCAATGACAA TGAGGGCCAC 
TCGGATGGCC TGGCAAGAAG AGGGAGAGGC GAGAGTTCAA GTGGCTATCC CGAGCCAAAG 
TACCCTGAAG ACAAACGGGA AGCGAGGAGT GACCAAGTGA AACCAGAAAA GGTGCCGAGA 
CGACGACGCA CCATGGCCGA CCCTGACTTC TGGACGCACA GTGATGATTA CTACAAATAC 
TGCGACGAAG ACTCTGACAG TGACAAAGAG TGGATTGCTG CTCTGCGTCG GAAATATCGA 
AGCCGAGAGC AAACCCTGTC CTCCAGTGGC GAAAGCTGGG AGACTCTGCC GGGGAAAGAA 
GAGCGGGAAC CTCCACAGGC TAAGGTGAGT GCCAGCACTG GCACCAGCCC TGGCCCCGGT 
GCTAGTGCCA GTGCCGGGGC TGGCGCCGGG GCCAGTGCTG GCAGCAATGG CAGCAATTAC 
CTTGAAGAAG TTCGAGAACC ATCTCTTCAG GAAGAGCAGG CATCCCTGGA AGAAGGAGAA 
ATTCCTTGGC TCCAGTACCA TGAGAATGAC AGTAGCAGTG AGGGGGATAA TGATTCTGGT 
CACGAGTTGA TGCAACCTGG GGTATTCATG CTGGATGGAA ACAACAACCT TGAAGATGAC 
TCCAGTGTGA GCGAAGACCT AGAAGTGGAT TGGAGCCTCT TTGATGGATT TGCAGATGGG 
TTAGGAGTGG CTGAAGCCAT TTCCTATGTG GACCCTCAGT TCCTCACCTA CATGGCACTT 
GAAGAACGCC TGGCCCAGGC AATGGAAACT GCCCTTGCGC ACTTGGAGTC TCTCGCAGTG 
GATGTAGAGG TGGCCAATCC ACCAGCAAGC AAGGAGAGCA TTGACGCTCT TCCCGAGATC 
CTGGTCACTG AAGATCATGG CGCAGTTGGT CAGGAGATGT GCTGCCCCAT CTGCTGTAGC 
GAATATGTGA AGGGGGAGGT GGCAACTGAG CTGCCGTGCC ACCACTATTT CCACAAGCCG 
TGTGTGTCCA TCTGGCTTCA GAAGTCAGGC ACCTGCCCCG TGTGCCGCTG CATGTTCCCT 
CCCCCACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660095.1
CDS	311..2242
Translation	MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERIASQRKTNSEVPMHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Target ORF information:

RefSeq Version	NM_145119.4
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145119.4

ORF Insert Sequence:

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGGTATCCG 
CCAAGAGAGT ACAGAGCTTC GGGTAGCAGA AGAGGAATGG CCTATGGACA TATTGACTCT 
TATGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGACC GCCAGTGAGA 
GGGAAAACTG GCAAGTTTAA AGATGATAAG CTGTATGACC CAGAGAAAGG GGCAAGGTCT 
TTGGCTGGGC CACCTCCACA TTTCTCTAGT TTTAGCCGTG ATGTGAGAGA GGAGCGAGAC 
AAGTTAGACC CAGTCCCTGC AGCAAGATGC TCAGCTAGCA GAGCTGACTT CCTGCCACAA 
AGTAGTGTGG CCTCACAGTC GTCTTCTGAA GGCAAGCTGG CTACAAAAGG TGACAGCTCG 
GAGAGGGAGA GAAGGGAGCA AAATTTACCT GCACGTCCCA GCAGGGCTCC TGTGAGTATT 
TGTGGTGGTG GGGAAAACAC CTCAAAGAGT GCAGAGGAAC CTGTGGTCAG GCCCAAAATC 
AGAAACCTGG CAAGTCCAAA CTGCGTGAAA CCAAAAATTT TTTTTGATAC TGATGATGAT 
GACGATATGC CACACAGTAC TTCCAGGTGG AGGGATACCG CCAATGACAA TGAGGGCCAC 
TCGGATGGCC TGGCAAGAAG AGGGAGAGGC GAGAGTTCAA GTGGCTATCC CGAGCCAAAG 
TACCCTGAAG ACAAACGGGA AGCGAGGAGT GACCAAGTGA AACCAGAAAA GGTGCCGAGA 
CGACGACGCA CCATGGCCGA CCCTGACTTC TGGACGCACA GTGATGATTA CTACAAATAC 
TGCGACGAAG ACTCTGACAG TGACAAAGAG TGGATTGCTG CTCTGCGTCG GAAATATCGA 
AGCCGAGAGC AAACCCTGTC CTCCAGTGGC GAAAGCTGGG AGACTCTGCC GGGGAAAGAA 
GAGCGGGAAC CTCCACAGGC TAAGGTGAGT GCCAGCACTG GCACCAGCCC TGGCCCCGGT 
GCTAGTGCCA GTGCCGGGGC TGGCGCCGGG GCCAGTGCTG GCAGCAATGG CAGCAATTAC 
CTTGAAGAAG TTCGAGAACC ATCTCTTCAG GAAGAGCAGG CATCCCTGGA AGAAGGAGAA 
ATTCCTTGGC TCCAGTACCA TGAGAATGAC AGTAGCAGTG AGGGGGATAA TGATTCTGGT 
CACGAGTTGA TGCAACCTGG GGTATTCATG CTGGATGGAA ACAACAACCT TGAAGATGAC 
TCCAGTGTGA GCGAAGACCT AGAAGTGGAT TGGAGCCTCT TTGATGGATT TGCAGATGGG 
TTAGGAGTGG CTGAAGCCAT TTCCTATGTG GACCCTCAGT TCCTCACCTA CATGGCACTT 
GAAGAACGCC TGGCCCAGGC AATGGAAACT GCCCTTGCGC ACTTGGAGTC TCTCGCAGTG 
GATGTAGAGG TGGCCAATCC ACCAGCAAGC AAGGAGAGCA TTGACGCTCT TCCCGAGATC 
CTGGTCACTG AAGATCATGG CGCAGTTGGT CAGGAGATGT GCTGCCCCAT CTGCTGTAGC 
GAATATGTGA AGGGGGAGGT GGCAACTGAG CTGCCGTGCC ACCACTATTT CCACAAGCCG 
TGTGTGTCCA TCTGGCTTCA GAAGTCAGGC ACCTGCCCCG TGTGCCGCTG CATGTTCCCT 
CCCCCACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA1 ( XM_005262292.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262349.1 Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X2, mRNA.

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CloneID	OHu04538
Clone ID Related Accession (Same CDS sequence)	XM_005262292.1 , NM_001032396.2 , NM_001032396.3
Accession Version	XM_005262292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCGCCATT TTCCACTACT 
CGTCGTAGTT GGGACGACAG CGAGAGTTCG GGAACCAACC TGAATATTGA TAATGAGGAC 
TATTCCAGGT ATCCGCCAAG AGAGTACAGA GCTTCGGGTA GCAGAAGAGG AATGGCCTAT 
GGACATATTG ACTCTTATGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG 
CGACCGCCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCAGAG 
AAAGGGGCAA GGTCTTTGGC TGGGCCACCT CCACATTTCT CTAGTTTTAG CCGTGATGTG 
AGAGAGGAGC GAGACAAGTT AGACCCAGTC CCTGCAGCAA GATGCTCAGC TAGCAGAGCT 
GACTTCCTGC CACAAAGTAG TGTGGCCTCA CAGTCGTCTT CTGAAGGCAA GCTGGCTACA 
AAAGGTGACA GCTCGGAGAG GGAGAGAAGG GAGCAAAATT TACCTGCACG TCCCAGCAGG 
GCTCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCTCAA AGAGTGCAGA GGAACCTGTG 
GTCAGGCCCA AAATCAGAAA CCTGGCAAGT CCAAACTGCG TGAAACCAAA AATTTTTTTT 
GATACTGATG ATGATGACGA TATGCCACAC AGTACTTCCA GGTGGAGGGA TACCGCCAAT 
GACAATGAGG GCCACTCGGA TGGCCTGGCA AGAAGAGGGA GAGGCGAGAG TTCAAGTGGC 
TATCCCGAGC CAAAGTACCC TGAAGACAAA CGGGAAGCGA GGAGTGACCA AGTGAAACCA 
GAAAAGGTGC CGAGACGACG ACGCACCATG GCCGACCCTG ACTTCTGGAC GCACAGTGAT 
GATTACTACA AATACTGCGA CGAAGACTCT GACAGTGACA AAGAGTGGAT TGCTGCTCTG 
CGTCGGAAAT ATCGAAGCCG AGAGCAAACC CTGTCCTCCA GTGGCGAAAG CTGGGAGACT 
CTGCCGGGGA AAGAAGAGCG GGAACCTCCA CAGGCTAAGG TGAGTGCCAG CACTGGCACC 
AGCCCTGGCC CCGGTGCTAG TGCCAGTGCC GGGGCTGGCG CCGGGGCCAG TGCTGGCAGC 
AATGGCAGCA ATTACCTTGA AGAAGTTCGA GAACCATCTC TTCAGGAAGA GCAGGCATCC 
CTGGAAGAAG GAGAAATTCC TTGGCTCCAG TACCATGAGA ATGACAGTAG CAGTGAGGGG 
GATAATGATT CTGGTCACGA GTTGATGCAA CCTGGGGTAT TCATGCTGGA TGGAAACAAC 
AACCTTGAAG ATGACTCCAG TGTGAGCGAA GACCTAGAAG TGGATTGGAG CCTCTTTGAT 
GGATTTGCAG ATGGGTTAGG AGTGGCTGAA GCCATTTCCT ATGTGGACCC TCAGTTCCTC 
ACCTACATGG CACTTGAAGA ACGCCTGGCC CAGGCAATGG AAACTGCCCT TGCGCACTTG 
GAGTCTCTCG CAGTGGATGT AGAGGTGGCC AATCCACCAG CAAGCAAGGA GAGCATTGAC 
GCTCTTCCCG AGATCCTGGT CACTGAAGAT CATGGCGCAG TTGGTCAGGA GATGTGCTGC 
CCCATCTGCT GTAGCGAATA TGTGAAGGGG GAGGTGGCAA CTGAGCTGCC GTGCCACCAC 
TATTTCCACA AGCCGTGTGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC 
CGCTGCATGT TCCCTCCCCC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262349.1
CDS	207..1973
Translation	MHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Target ORF information:

RefSeq Version	XM_005262292.1
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262292.1

ORF Insert Sequence:

ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GATCGCCATT TTCCACTACT 
CGTCGTAGTT GGGACGACAG CGAGAGTTCG GGAACCAACC TGAATATTGA TAATGAGGAC 
TATTCCAGGT ATCCGCCAAG AGAGTACAGA GCTTCGGGTA GCAGAAGAGG AATGGCCTAT 
GGACATATTG ACTCTTATGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG 
CGACCGCCAG TGAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCTGTA TGACCCAGAG 
AAAGGGGCAA GGTCTTTGGC TGGGCCACCT CCACATTTCT CTAGTTTTAG CCGTGATGTG 
AGAGAGGAGC GAGACAAGTT AGACCCAGTC CCTGCAGCAA GATGCTCAGC TAGCAGAGCT 
GACTTCCTGC CACAAAGTAG TGTGGCCTCA CAGTCGTCTT CTGAAGGCAA GCTGGCTACA 
AAAGGTGACA GCTCGGAGAG GGAGAGAAGG GAGCAAAATT TACCTGCACG TCCCAGCAGG 
GCTCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCTCAA AGAGTGCAGA GGAACCTGTG 
GTCAGGCCCA AAATCAGAAA CCTGGCAAGT CCAAACTGCG TGAAACCAAA AATTTTTTTT 
GATACTGATG ATGATGACGA TATGCCACAC AGTACTTCCA GGTGGAGGGA TACCGCCAAT 
GACAATGAGG GCCACTCGGA TGGCCTGGCA AGAAGAGGGA GAGGCGAGAG TTCAAGTGGC 
TATCCCGAGC CAAAGTACCC TGAAGACAAA CGGGAAGCGA GGAGTGACCA AGTGAAACCA 
GAAAAGGTGC CGAGACGACG ACGCACCATG GCCGACCCTG ACTTCTGGAC GCACAGTGAT 
GATTACTACA AATACTGCGA CGAAGACTCT GACAGTGACA AAGAGTGGAT TGCTGCTCTG 
CGTCGGAAAT ATCGAAGCCG AGAGCAAACC CTGTCCTCCA GTGGCGAAAG CTGGGAGACT 
CTGCCGGGGA AAGAAGAGCG GGAACCTCCA CAGGCTAAGG TGAGTGCCAG CACTGGCACC 
AGCCCTGGCC CCGGTGCTAG TGCCAGTGCC GGGGCTGGCG CCGGGGCCAG TGCTGGCAGC 
AATGGCAGCA ATTACCTTGA AGAAGTTCGA GAACCATCTC TTCAGGAAGA GCAGGCATCC 
CTGGAAGAAG GAGAAATTCC TTGGCTCCAG TACCATGAGA ATGACAGTAG CAGTGAGGGG 
GATAATGATT CTGGTCACGA GTTGATGCAA CCTGGGGTAT TCATGCTGGA TGGAAACAAC 
AACCTTGAAG ATGACTCCAG TGTGAGCGAA GACCTAGAAG TGGATTGGAG CCTCTTTGAT 
GGATTTGCAG ATGGGTTAGG AGTGGCTGAA GCCATTTCCT ATGTGGACCC TCAGTTCCTC 
ACCTACATGG CACTTGAAGA ACGCCTGGCC CAGGCAATGG AAACTGCCCT TGCGCACTTG 
GAGTCTCTCG CAGTGGATGT AGAGGTGGCC AATCCACCAG CAAGCAAGGA GAGCATTGAC 
GCTCTTCCCG AGATCCTGGT CACTGAAGAT CATGGCGCAG TTGGTCAGGA GATGTGCTGC 
CCCATCTGCT GTAGCGAATA TGTGAAGGGG GAGGTGGCAA CTGAGCTGCC GTGCCACCAC 
TATTTCCACA AGCCGTGTGT GTCCATCTGG CTTCAGAAGT CAGGCACCTG CCCCGTGTGC 
CGCTGCATGT TCCCTCCCCC ACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA1 ( XM_011531011.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529313.1 Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X1, mRNA.

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CloneID	OHu55728
Clone ID Related Accession (Same CDS sequence)	NM_145119.3 , XM_011531011.2 , XM_011531011.1 , NM_145119.4
Accession Version	XM_011531011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGGTATCCG 
CCAAGAGAGT ACAGAGCTTC GGGTAGCAGA AGAGGAATGG CCTATGGACA TATTGACTCT 
TATGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGACC GCCAGTGAGA 
GGGAAAACTG GCAAGTTTAA AGATGATAAG CTGTATGACC CAGAGAAAGG GGCAAGGTCT 
TTGGCTGGGC CACCTCCACA TTTCTCTAGT TTTAGCCGTG ATGTGAGAGA GGAGCGAGAC 
AAGTTAGACC CAGTCCCTGC AGCAAGATGC TCAGCTAGCA GAGCTGACTT CCTGCCACAA 
AGTAGTGTGG CCTCACAGTC GTCTTCTGAA GGCAAGCTGG CTACAAAAGG TGACAGCTCG 
GAGAGGGAGA GAAGGGAGCA AAATTTACCT GCACGTCCCA GCAGGGCTCC TGTGAGTATT 
TGTGGTGGTG GGGAAAACAC CTCAAAGAGT GCAGAGGAAC CTGTGGTCAG GCCCAAAATC 
AGAAACCTGG CAAGTCCAAA CTGCGTGAAA CCAAAAATTT TTTTTGATAC TGATGATGAT 
GACGATATGC CACACAGTAC TTCCAGGTGG AGGGATACCG CCAATGACAA TGAGGGCCAC 
TCGGATGGCC TGGCAAGAAG AGGGAGAGGC GAGAGTTCAA GTGGCTATCC CGAGCCAAAG 
TACCCTGAAG ACAAACGGGA AGCGAGGAGT GACCAAGTGA AACCAGAAAA GGTGCCGAGA 
CGACGACGCA CCATGGCCGA CCCTGACTTC TGGACGCACA GTGATGATTA CTACAAATAC 
TGCGACGAAG ACTCTGACAG TGACAAAGAG TGGATTGCTG CTCTGCGTCG GAAATATCGA 
AGCCGAGAGC AAACCCTGTC CTCCAGTGGC GAAAGCTGGG AGACTCTGCC GGGGAAAGAA 
GAGCGGGAAC CTCCACAGGC TAAGGTGAGT GCCAGCACTG GCACCAGCCC TGGCCCCGGT 
GCTAGTGCCA GTGCCGGGGC TGGCGCCGGG GCCAGTGCTG GCAGCAATGG CAGCAATTAC 
CTTGAAGAAG TTCGAGAACC ATCTCTTCAG GAAGAGCAGG CATCCCTGGA AGAAGGAGAA 
ATTCCTTGGC TCCAGTACCA TGAGAATGAC AGTAGCAGTG AGGGGGATAA TGATTCTGGT 
CACGAGTTGA TGCAACCTGG GGTATTCATG CTGGATGGAA ACAACAACCT TGAAGATGAC 
TCCAGTGTGA GCGAAGACCT AGAAGTGGAT TGGAGCCTCT TTGATGGATT TGCAGATGGG 
TTAGGAGTGG CTGAAGCCAT TTCCTATGTG GACCCTCAGT TCCTCACCTA CATGGCACTT 
GAAGAACGCC TGGCCCAGGC AATGGAAACT GCCCTTGCGC ACTTGGAGTC TCTCGCAGTG 
GATGTAGAGG TGGCCAATCC ACCAGCAAGC AAGGAGAGCA TTGACGCTCT TCCCGAGATC 
CTGGTCACTG AAGATCATGG CGCAGTTGGT CAGGAGATGT GCTGCCCCAT CTGCTGTAGC 
GAATATGTGA AGGGGGAGGT GGCAACTGAG CTGCCGTGCC ACCACTATTT CCACAAGCCG 
TGTGTGTCCA TCTGGCTTCA GAAGTCAGGC ACCTGCCCCG TGTGCCGCTG CATGTTCCCT 
CCCCCACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529313.1
CDS	187..2118
Translation	MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERIASQRKTNSEVPMHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Target ORF information:

RefSeq Version	XM_011531011.2
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531011.2

ORF Insert Sequence:

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGGTATCCG 
CCAAGAGAGT ACAGAGCTTC GGGTAGCAGA AGAGGAATGG CCTATGGACA TATTGACTCT 
TATGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGACC GCCAGTGAGA 
GGGAAAACTG GCAAGTTTAA AGATGATAAG CTGTATGACC CAGAGAAAGG GGCAAGGTCT 
TTGGCTGGGC CACCTCCACA TTTCTCTAGT TTTAGCCGTG ATGTGAGAGA GGAGCGAGAC 
AAGTTAGACC CAGTCCCTGC AGCAAGATGC TCAGCTAGCA GAGCTGACTT CCTGCCACAA 
AGTAGTGTGG CCTCACAGTC GTCTTCTGAA GGCAAGCTGG CTACAAAAGG TGACAGCTCG 
GAGAGGGAGA GAAGGGAGCA AAATTTACCT GCACGTCCCA GCAGGGCTCC TGTGAGTATT 
TGTGGTGGTG GGGAAAACAC CTCAAAGAGT GCAGAGGAAC CTGTGGTCAG GCCCAAAATC 
AGAAACCTGG CAAGTCCAAA CTGCGTGAAA CCAAAAATTT TTTTTGATAC TGATGATGAT 
GACGATATGC CACACAGTAC TTCCAGGTGG AGGGATACCG CCAATGACAA TGAGGGCCAC 
TCGGATGGCC TGGCAAGAAG AGGGAGAGGC GAGAGTTCAA GTGGCTATCC CGAGCCAAAG 
TACCCTGAAG ACAAACGGGA AGCGAGGAGT GACCAAGTGA AACCAGAAAA GGTGCCGAGA 
CGACGACGCA CCATGGCCGA CCCTGACTTC TGGACGCACA GTGATGATTA CTACAAATAC 
TGCGACGAAG ACTCTGACAG TGACAAAGAG TGGATTGCTG CTCTGCGTCG GAAATATCGA 
AGCCGAGAGC AAACCCTGTC CTCCAGTGGC GAAAGCTGGG AGACTCTGCC GGGGAAAGAA 
GAGCGGGAAC CTCCACAGGC TAAGGTGAGT GCCAGCACTG GCACCAGCCC TGGCCCCGGT 
GCTAGTGCCA GTGCCGGGGC TGGCGCCGGG GCCAGTGCTG GCAGCAATGG CAGCAATTAC 
CTTGAAGAAG TTCGAGAACC ATCTCTTCAG GAAGAGCAGG CATCCCTGGA AGAAGGAGAA 
ATTCCTTGGC TCCAGTACCA TGAGAATGAC AGTAGCAGTG AGGGGGATAA TGATTCTGGT 
CACGAGTTGA TGCAACCTGG GGTATTCATG CTGGATGGAA ACAACAACCT TGAAGATGAC 
TCCAGTGTGA GCGAAGACCT AGAAGTGGAT TGGAGCCTCT TTGATGGATT TGCAGATGGG 
TTAGGAGTGG CTGAAGCCAT TTCCTATGTG GACCCTCAGT TCCTCACCTA CATGGCACTT 
GAAGAACGCC TGGCCCAGGC AATGGAAACT GCCCTTGCGC ACTTGGAGTC TCTCGCAGTG 
GATGTAGAGG TGGCCAATCC ACCAGCAAGC AAGGAGAGCA TTGACGCTCT TCCCGAGATC 
CTGGTCACTG AAGATCATGG CGCAGTTGGT CAGGAGATGT GCTGCCCCAT CTGCTGTAGC 
GAATATGTGA AGGGGGAGGT GGCAACTGAG CTGCCGTGCC ACCACTATTT CCACAAGCCG 
TGTGTGTCCA TCTGGCTTCA GAAGTCAGGC ACCTGCCCCG TGTGCCGCTG CATGTTCCCT 
CCCCCACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA1 ( XM_011531011.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529313.1 Homo sapiens praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X1, mRNA.

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CloneID	OHu55728
Clone ID Related Accession (Same CDS sequence)	NM_145119.3 , XM_011531011.2 , XM_011531011.1 , NM_145119.4
Accession Version	XM_011531011.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	E3 ubiquitin-protein ligase Praja-1 isoform X2
Comment

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGGTATCCG 
CCAAGAGAGT ACAGAGCTTC GGGTAGCAGA AGAGGAATGG CCTATGGACA TATTGACTCT 
TATGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGACC GCCAGTGAGA 
GGGAAAACTG GCAAGTTTAA AGATGATAAG CTGTATGACC CAGAGAAAGG GGCAAGGTCT 
TTGGCTGGGC CACCTCCACA TTTCTCTAGT TTTAGCCGTG ATGTGAGAGA GGAGCGAGAC 
AAGTTAGACC CAGTCCCTGC AGCAAGATGC TCAGCTAGCA GAGCTGACTT CCTGCCACAA 
AGTAGTGTGG CCTCACAGTC GTCTTCTGAA GGCAAGCTGG CTACAAAAGG TGACAGCTCG 
GAGAGGGAGA GAAGGGAGCA AAATTTACCT GCACGTCCCA GCAGGGCTCC TGTGAGTATT 
TGTGGTGGTG GGGAAAACAC CTCAAAGAGT GCAGAGGAAC CTGTGGTCAG GCCCAAAATC 
AGAAACCTGG CAAGTCCAAA CTGCGTGAAA CCAAAAATTT TTTTTGATAC TGATGATGAT 
GACGATATGC CACACAGTAC TTCCAGGTGG AGGGATACCG CCAATGACAA TGAGGGCCAC 
TCGGATGGCC TGGCAAGAAG AGGGAGAGGC GAGAGTTCAA GTGGCTATCC CGAGCCAAAG 
TACCCTGAAG ACAAACGGGA AGCGAGGAGT GACCAAGTGA AACCAGAAAA GGTGCCGAGA 
CGACGACGCA CCATGGCCGA CCCTGACTTC TGGACGCACA GTGATGATTA CTACAAATAC 
TGCGACGAAG ACTCTGACAG TGACAAAGAG TGGATTGCTG CTCTGCGTCG GAAATATCGA 
AGCCGAGAGC AAACCCTGTC CTCCAGTGGC GAAAGCTGGG AGACTCTGCC GGGGAAAGAA 
GAGCGGGAAC CTCCACAGGC TAAGGTGAGT GCCAGCACTG GCACCAGCCC TGGCCCCGGT 
GCTAGTGCCA GTGCCGGGGC TGGCGCCGGG GCCAGTGCTG GCAGCAATGG CAGCAATTAC 
CTTGAAGAAG TTCGAGAACC ATCTCTTCAG GAAGAGCAGG CATCCCTGGA AGAAGGAGAA 
ATTCCTTGGC TCCAGTACCA TGAGAATGAC AGTAGCAGTG AGGGGGATAA TGATTCTGGT 
CACGAGTTGA TGCAACCTGG GGTATTCATG CTGGATGGAA ACAACAACCT TGAAGATGAC 
TCCAGTGTGA GCGAAGACCT AGAAGTGGAT TGGAGCCTCT TTGATGGATT TGCAGATGGG 
TTAGGAGTGG CTGAAGCCAT TTCCTATGTG GACCCTCAGT TCCTCACCTA CATGGCACTT 
GAAGAACGCC TGGCCCAGGC AATGGAAACT GCCCTTGCGC ACTTGGAGTC TCTCGCAGTG 
GATGTAGAGG TGGCCAATCC ACCAGCAAGC AAGGAGAGCA TTGACGCTCT TCCCGAGATC 
CTGGTCACTG AAGATCATGG CGCAGTTGGT CAGGAGATGT GCTGCCCCAT CTGCTGTAGC 
GAATATGTGA AGGGGGAGGT GGCAACTGAG CTGCCGTGCC ACCACTATTT CCACAAGCCG 
TGTGTGTCCA TCTGGCTTCA GAAGTCAGGC ACCTGCCCCG TGTGCCGCTG CATGTTCCCT 
CCCCCACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011529313.1

Translation

MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERIASQRKTNSEVPMHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Target ORF information:

RefSeq Version	XM_011531011.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531011.1

ORF Insert Sequence:

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGGTATCCG 
CCAAGAGAGT ACAGAGCTTC GGGTAGCAGA AGAGGAATGG CCTATGGACA TATTGACTCT 
TATGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGACC GCCAGTGAGA 
GGGAAAACTG GCAAGTTTAA AGATGATAAG CTGTATGACC CAGAGAAAGG GGCAAGGTCT 
TTGGCTGGGC CACCTCCACA TTTCTCTAGT TTTAGCCGTG ATGTGAGAGA GGAGCGAGAC 
AAGTTAGACC CAGTCCCTGC AGCAAGATGC TCAGCTAGCA GAGCTGACTT CCTGCCACAA 
AGTAGTGTGG CCTCACAGTC GTCTTCTGAA GGCAAGCTGG CTACAAAAGG TGACAGCTCG 
GAGAGGGAGA GAAGGGAGCA AAATTTACCT GCACGTCCCA GCAGGGCTCC TGTGAGTATT 
TGTGGTGGTG GGGAAAACAC CTCAAAGAGT GCAGAGGAAC CTGTGGTCAG GCCCAAAATC 
AGAAACCTGG CAAGTCCAAA CTGCGTGAAA CCAAAAATTT TTTTTGATAC TGATGATGAT 
GACGATATGC CACACAGTAC TTCCAGGTGG AGGGATACCG CCAATGACAA TGAGGGCCAC 
TCGGATGGCC TGGCAAGAAG AGGGAGAGGC GAGAGTTCAA GTGGCTATCC CGAGCCAAAG 
TACCCTGAAG ACAAACGGGA AGCGAGGAGT GACCAAGTGA AACCAGAAAA GGTGCCGAGA 
CGACGACGCA CCATGGCCGA CCCTGACTTC TGGACGCACA GTGATGATTA CTACAAATAC 
TGCGACGAAG ACTCTGACAG TGACAAAGAG TGGATTGCTG CTCTGCGTCG GAAATATCGA 
AGCCGAGAGC AAACCCTGTC CTCCAGTGGC GAAAGCTGGG AGACTCTGCC GGGGAAAGAA 
GAGCGGGAAC CTCCACAGGC TAAGGTGAGT GCCAGCACTG GCACCAGCCC TGGCCCCGGT 
GCTAGTGCCA GTGCCGGGGC TGGCGCCGGG GCCAGTGCTG GCAGCAATGG CAGCAATTAC 
CTTGAAGAAG TTCGAGAACC ATCTCTTCAG GAAGAGCAGG CATCCCTGGA AGAAGGAGAA 
ATTCCTTGGC TCCAGTACCA TGAGAATGAC AGTAGCAGTG AGGGGGATAA TGATTCTGGT 
CACGAGTTGA TGCAACCTGG GGTATTCATG CTGGATGGAA ACAACAACCT TGAAGATGAC 
TCCAGTGTGA GCGAAGACCT AGAAGTGGAT TGGAGCCTCT TTGATGGATT TGCAGATGGG 
TTAGGAGTGG CTGAAGCCAT TTCCTATGTG GACCCTCAGT TCCTCACCTA CATGGCACTT 
GAAGAACGCC TGGCCCAGGC AATGGAAACT GCCCTTGCGC ACTTGGAGTC TCTCGCAGTG 
GATGTAGAGG TGGCCAATCC ACCAGCAAGC AAGGAGAGCA TTGACGCTCT TCCCGAGATC 
CTGGTCACTG AAGATCATGG CGCAGTTGGT CAGGAGATGT GCTGCCCCAT CTGCTGTAGC 
GAATATGTGA AGGGGGAGGT GGCAACTGAG CTGCCGTGCC ACCACTATTT CCACAAGCCG 
TGTGTGTCCA TCTGGCTTCA GAAGTCAGGC ACCTGCCCCG TGTGCCGCTG CATGTTCCCT 
CCCCCACTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA1 ( NM_022368.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071763.2 Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 3, mRNA.

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CloneID	OHu20845
Clone ID Related Accession (Same CDS sequence)	NM_022368.5 , NM_022368.4
Accession Version	NM_022368.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC349747.1, AF264620.1, AL157699.13 and BM127146.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_022368.4. Transcript Variant: This variant (3) lacks an in-frame segment of the 5' coding region, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF264620.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGTACTTCC 
AGGTGGAGGG ATACCGCCAA TGACAATGAG GGCCACTCGG ATGGCCTGGC AAGAAGAGGG 
AGAGGCGAGA GTTCAAGTGG CTATCCCGAG CCAAAGTACC CTGAAGACAA ACGGGAAGCG 
AGGAGTGACC AAGTGAAACC AGAAAAGGTG CCGAGACGAC GACGCACCAT GGCCGACCCT 
GACTTCTGGA CGCACAGTGA TGATTACTAC AAATACTGCG ACGAAGACTC TGACAGTGAC 
AAAGAGTGGA TTGCTGCTCT GCGTCGGAAA TATCGAAGCC GAGAGCAAAC CCTGTCCTCC 
AGTGGCGAAA GCTGGGAGAC TCTGCCGGGG AAAGAAGAGC GGGAACCTCC ACAGGCTAAG 
GTGAGTGCCA GCACTGGCAC CAGCCCTGGC CCCGGTGCTA GTGCCAGTGC CGGGGCTGGC 
GCCGGGGCCA GTGCTGGCAG CAATGGCAGC AATTACCTTG AAGAAGTTCG AGAACCATCT 
CTTCAGGAAG AGCAGGCATC CCTGGAAGAA GGAGAAATTC CTTGGCTCCA GTACCATGAG 
AATGACAGTA GCAGTGAGGG GGATAATGAT TCTGGTCACG AGTTGATGCA ACCTGGGGTA 
TTCATGCTGG ATGGAAACAA CAACCTTGAA GATGACTCCA GTGTGAGCGA AGACCTAGAA 
GTGGATTGGA GCCTCTTTGA TGGATTTGCA GATGGGTTAG GAGTGGCTGA AGCCATTTCC 
TATGTGGACC CTCAGTTCCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG 
GAAACTGCCC TTGCGCACTT GGAGTCTCTC GCAGTGGATG TAGAGGTGGC CAATCCACCA 
GCAAGCAAGG AGAGCATTGA CGCTCTTCCC GAGATCCTGG TCACTGAAGA TCATGGCGCA 
GTTGGTCAGG AGATGTGCTG CCCCATCTGC TGTAGCGAAT ATGTGAAGGG GGAGGTGGCA 
ACTGAGCTGC CGTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAG 
TCAGGCACCT GCCCCGTGTG CCGCTGCATG TTCCCTCCCC CACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071763.2
CDS	311..1678
Translation	MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERIASQRKTNSEVPMHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Target ORF information:

RefSeq Version	NM_022368.5
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022368.5

ORF Insert Sequence:

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGTACTTCC 
AGGTGGAGGG ATACCGCCAA TGACAATGAG GGCCACTCGG ATGGCCTGGC AAGAAGAGGG 
AGAGGCGAGA GTTCAAGTGG CTATCCCGAG CCAAAGTACC CTGAAGACAA ACGGGAAGCG 
AGGAGTGACC AAGTGAAACC AGAAAAGGTG CCGAGACGAC GACGCACCAT GGCCGACCCT 
GACTTCTGGA CGCACAGTGA TGATTACTAC AAATACTGCG ACGAAGACTC TGACAGTGAC 
AAAGAGTGGA TTGCTGCTCT GCGTCGGAAA TATCGAAGCC GAGAGCAAAC CCTGTCCTCC 
AGTGGCGAAA GCTGGGAGAC TCTGCCGGGG AAAGAAGAGC GGGAACCTCC ACAGGCTAAG 
GTGAGTGCCA GCACTGGCAC CAGCCCTGGC CCCGGTGCTA GTGCCAGTGC CGGGGCTGGC 
GCCGGGGCCA GTGCTGGCAG CAATGGCAGC AATTACCTTG AAGAAGTTCG AGAACCATCT 
CTTCAGGAAG AGCAGGCATC CCTGGAAGAA GGAGAAATTC CTTGGCTCCA GTACCATGAG 
AATGACAGTA GCAGTGAGGG GGATAATGAT TCTGGTCACG AGTTGATGCA ACCTGGGGTA 
TTCATGCTGG ATGGAAACAA CAACCTTGAA GATGACTCCA GTGTGAGCGA AGACCTAGAA 
GTGGATTGGA GCCTCTTTGA TGGATTTGCA GATGGGTTAG GAGTGGCTGA AGCCATTTCC 
TATGTGGACC CTCAGTTCCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG 
GAAACTGCCC TTGCGCACTT GGAGTCTCTC GCAGTGGATG TAGAGGTGGC CAATCCACCA 
GCAAGCAAGG AGAGCATTGA CGCTCTTCCC GAGATCCTGG TCACTGAAGA TCATGGCGCA 
GTTGGTCAGG AGATGTGCTG CCCCATCTGC TGTAGCGAAT ATGTGAAGGG GGAGGTGGCA 
ACTGAGCTGC CGTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAG 
TCAGGCACCT GCCCCGTGTG CCGCTGCATG TTCCCTCCCC CACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA1 ( NM_022368.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071763.2 Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 3, mRNA.

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CloneID	OHu20845
Clone ID Related Accession (Same CDS sequence)	NM_022368.5 , NM_022368.4
Accession Version	NM_022368.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC349747.1, AF264620.1, AL157699.13 and BM127146.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2010 this sequence version replaced NM_022368.3. Transcript Variant: This variant (3) lacks an in-frame segment of the 5' coding region, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AF264620.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGTACTTCC 
AGGTGGAGGG ATACCGCCAA TGACAATGAG GGCCACTCGG ATGGCCTGGC AAGAAGAGGG 
AGAGGCGAGA GTTCAAGTGG CTATCCCGAG CCAAAGTACC CTGAAGACAA ACGGGAAGCG 
AGGAGTGACC AAGTGAAACC AGAAAAGGTG CCGAGACGAC GACGCACCAT GGCCGACCCT 
GACTTCTGGA CGCACAGTGA TGATTACTAC AAATACTGCG ACGAAGACTC TGACAGTGAC 
AAAGAGTGGA TTGCTGCTCT GCGTCGGAAA TATCGAAGCC GAGAGCAAAC CCTGTCCTCC 
AGTGGCGAAA GCTGGGAGAC TCTGCCGGGG AAAGAAGAGC GGGAACCTCC ACAGGCTAAG 
GTGAGTGCCA GCACTGGCAC CAGCCCTGGC CCCGGTGCTA GTGCCAGTGC CGGGGCTGGC 
GCCGGGGCCA GTGCTGGCAG CAATGGCAGC AATTACCTTG AAGAAGTTCG AGAACCATCT 
CTTCAGGAAG AGCAGGCATC CCTGGAAGAA GGAGAAATTC CTTGGCTCCA GTACCATGAG 
AATGACAGTA GCAGTGAGGG GGATAATGAT TCTGGTCACG AGTTGATGCA ACCTGGGGTA 
TTCATGCTGG ATGGAAACAA CAACCTTGAA GATGACTCCA GTGTGAGCGA AGACCTAGAA 
GTGGATTGGA GCCTCTTTGA TGGATTTGCA GATGGGTTAG GAGTGGCTGA AGCCATTTCC 
TATGTGGACC CTCAGTTCCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG 
GAAACTGCCC TTGCGCACTT GGAGTCTCTC GCAGTGGATG TAGAGGTGGC CAATCCACCA 
GCAAGCAAGG AGAGCATTGA CGCTCTTCCC GAGATCCTGG TCACTGAAGA TCATGGCGCA 
GTTGGTCAGG AGATGTGCTG CCCCATCTGC TGTAGCGAAT ATGTGAAGGG GGAGGTGGCA 
ACTGAGCTGC CGTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAG 
TCAGGCACCT GCCCCGTGTG CCGCTGCATG TTCCCTCCCC CACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071763.2
CDS	380..1747
Translation	MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERIASQRKTNSEVPMHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Target ORF information:

RefSeq Version	NM_022368.4
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 1 (PJA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022368.4

ORF Insert Sequence:

ATGGGTCAGG AATCTAGCAA GCCTGTATGG CCCAATCCAA CAGGAGGGTA TCAGTCCAAT 
ACAGGTAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCACC CACGAGCCAG 
CGGGAAAGGA TTGCCAGCCA GAGAAAGACG AACTCCGAAG TCCCAATGCA CAGATCAGCC 
CCCAGTCAAA CCACCAAGAG GAGCCGATCG CCATTTTCCA CTACTCGTCG TAGTTGGGAC 
GACAGCGAGA GTTCGGGAAC CAACCTGAAT ATTGATAATG AGGACTATTC CAGTACTTCC 
AGGTGGAGGG ATACCGCCAA TGACAATGAG GGCCACTCGG ATGGCCTGGC AAGAAGAGGG 
AGAGGCGAGA GTTCAAGTGG CTATCCCGAG CCAAAGTACC CTGAAGACAA ACGGGAAGCG 
AGGAGTGACC AAGTGAAACC AGAAAAGGTG CCGAGACGAC GACGCACCAT GGCCGACCCT 
GACTTCTGGA CGCACAGTGA TGATTACTAC AAATACTGCG ACGAAGACTC TGACAGTGAC 
AAAGAGTGGA TTGCTGCTCT GCGTCGGAAA TATCGAAGCC GAGAGCAAAC CCTGTCCTCC 
AGTGGCGAAA GCTGGGAGAC TCTGCCGGGG AAAGAAGAGC GGGAACCTCC ACAGGCTAAG 
GTGAGTGCCA GCACTGGCAC CAGCCCTGGC CCCGGTGCTA GTGCCAGTGC CGGGGCTGGC 
GCCGGGGCCA GTGCTGGCAG CAATGGCAGC AATTACCTTG AAGAAGTTCG AGAACCATCT 
CTTCAGGAAG AGCAGGCATC CCTGGAAGAA GGAGAAATTC CTTGGCTCCA GTACCATGAG 
AATGACAGTA GCAGTGAGGG GGATAATGAT TCTGGTCACG AGTTGATGCA ACCTGGGGTA 
TTCATGCTGG ATGGAAACAA CAACCTTGAA GATGACTCCA GTGTGAGCGA AGACCTAGAA 
GTGGATTGGA GCCTCTTTGA TGGATTTGCA GATGGGTTAG GAGTGGCTGA AGCCATTTCC 
TATGTGGACC CTCAGTTCCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG 
GAAACTGCCC TTGCGCACTT GGAGTCTCTC GCAGTGGATG TAGAGGTGGC CAATCCACCA 
GCAAGCAAGG AGAGCATTGA CGCTCTTCCC GAGATCCTGG TCACTGAAGA TCATGGCGCA 
GTTGGTCAGG AGATGTGCTG CCCCATCTGC TGTAGCGAAT ATGTGAAGGG GGAGGTGGCA 
ACTGAGCTGC CGTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAG 
TCAGGCACCT GCCCCGTGTG CCGCTGCATG TTCCCTCCCC CACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PJA1
Entrez Gene ID	64219
Full Name	praja ring finger ubiquitin ligase 1
Synonyms	PRAJA1,RNF70
General protein information	Preferred Names praja ring finger ubiquitin ligase 1 Names E3 ubiquitin-protein ligase Praja-1 RING finger protein 70 RING-type E3 ubiquitin transferase Praja-1 praja 1 praja ring finger 1, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq13.1
Summary	This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked cognitive disability disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010].
Disorder MIM:	300420

	LOC695038	XP_002806315.1
Homo sapiens (human)	PJA1	NP_660095.1
Mus musculus (house mouse)	Pja1	NP_001076579.1
Rattus norvegicus (Norway rat)	LOC683077	NP_001094476.1
Canis lupus familiaris (dog)	PJA1	XP_005641498.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PJA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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