PJA1 cDNA ORF clone, Canis lupus familiaris(dog)

The following PJA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PJA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe32479 XM_014111872.2
Latest version!
Canis lupus familiaris praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe32479 XM_005641441.3
Latest version!
Canis lupus familiaris praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe32480 XM_005641442.3
Latest version!
Canis lupus familiaris praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe32481 XM_014111873.2
Latest version!
Canis lupus familiaris praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
View Old Accession Versions >>
OCe32481 XM_014111873.1 Canis lupus familiaris praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OCe32479 XM_005641441.2 Canis lupus familiaris praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe32480 XM_005641442.2 Canis lupus familiaris praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe32479 XM_014111872.1 Canis lupus familiaris praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe32479
    Clone ID Related Accession (Same CDS sequence) XM_005641441.2 , XM_005641441.3 , XM_014111872.1 , XM_014111872.2
    Accession Version XM_005641441.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGGTACCCA
    CCGAGAGAGT ACAGGGCTTC AGGTAGCAGA AGAGGATTGG CTTATGGACA TGTTGACTCT
    TTTGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGAGC ACCAGTTAGA
    GGGAAAACTG GCAAGTTTAA AGATGATAAG CCCTACGACC CAGAGAAAGG GGCCAGGTCT
    TTGGCTGGGG TGGCTCCACA GTTCTCTAGC TTTAAGCGTG ATGTGAGAGA GGAGCTTGAC
    AAGTTAGACC CGGCCCCTGC AGCAAGATGC TCTGTTAGCA GAGCTGAGTT CCTGCAGCAA
    AATAGCATGG CCTCTCAGAC ATCTGCTGAA GGCAAGGTGG CTACCCGTGG TGACAGCCGG
    GAGAGTCAGA GACGGGAGCA GAATTTCCCT GCACATCCCA GCAGGGATCC TGTGAGTATT
    TGTGGTGGTG GGGAAAACAC CCCCAAGAGT GCAGAGGAGC CGGTGGTGAG GCCTAAAATC
    AGAAATCTGG CGAGCCCCAA CTGCGTGAAA CCAAAAATAT TTTTTGATAC TGATGATGAT
    GATGATATGC CACATAGTAC TTCCAGGTGG AGGGATACAG CCAACGCTGA AGGCCACTCG
    GATGGCCTAG CAAGAAGAGC CAGAGGTGAG AGTTCAGGTG GCTACTCTGA GCTGAAGTAC
    CCCGAAGACA AGAGGGAAGC CAGGAATGAC CAAGTGAAGC CAGAAAAGGT ACCAAGACGG
    CGGCGAACCA TGGCTGACCC TGACTTCTGG ACGTACAGCG ACGATTACTA CAAATACTGT
    GATGAAGACT CTGACAGTGA CAAAGAGTGG ACTGCTGCTC TGCGGCGGAA GTATCGAGGT
    CGAGAGCAGA ATCTGTCGTC CAGTGGTGAA AGCTGGGAGA CTCTGCCAGG GAAAGAGGAG
    CACGACTCTG AGCAGGTCAG AGTGAATGCT GGCGCCGGCG CCAGTGCCAG CCCGGGTACC
    GGCGCCAGCG CCGGCAGCAA CAGCAGCAGT GAACTGGAAG ACCTCCGAGG ACCACCTCTT
    CAGGAAGAGG AACACGCATC CCCCAGAGAA CGAGAAGCTC CTTGGCTCCA GTACAACGAA
    AATGAGAGTA GCAGTGAGGG GGATAATGAA TCTGCTCAGG AGTATCTACA GCCTGGGGTG
    TTCATGCTCG ATGGAAACAA CAATCTTGAA GATGACTCCA GCGTGAGTGA AGACCTAGAA
    GTGGATTGGA GCCTCTTTGA TGGGTTCGCA GATGGCTTGG GGGTGGCCGA AGCCATTTCC
    TACGTGGATC CTCAGTTTCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG
    GAAACTGCCC TGGCGCACCT AGAGTCTCTC GCAGTGGACG TGGAGGTGGC CAACCCACCG
    GCGAGCAAGG AGAGCATCGA CACTCTCCCT GAGATCCTGG TCACTGAAGA TCACAGTGCA
    GTGGGGCAGG AAATGTGTTG CCCCATCTGT TGTAGTGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTGC CTTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAA
    TCAGGCACCT GCCCTGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641498.1
    CDS220..2127
    Translation

    Target ORF information:

    RefSeq Version XM_005641441.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641441.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGGTACCCA
    CCGAGAGAGT ACAGGGCTTC AGGTAGCAGA AGAGGATTGG CTTATGGACA TGTTGACTCT
    TTTGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGAGC ACCAGTTAGA
    GGGAAAACTG GCAAGTTTAA AGATGATAAG CCCTACGACC CAGAGAAAGG GGCCAGGTCT
    TTGGCTGGGG TGGCTCCACA GTTCTCTAGC TTTAAGCGTG ATGTGAGAGA GGAGCTTGAC
    AAGTTAGACC CGGCCCCTGC AGCAAGATGC TCTGTTAGCA GAGCTGAGTT CCTGCAGCAA
    AATAGCATGG CCTCTCAGAC ATCTGCTGAA GGCAAGGTGG CTACCCGTGG TGACAGCCGG
    GAGAGTCAGA GACGGGAGCA GAATTTCCCT GCACATCCCA GCAGGGATCC TGTGAGTATT
    TGTGGTGGTG GGGAAAACAC CCCCAAGAGT GCAGAGGAGC CGGTGGTGAG GCCTAAAATC
    AGAAATCTGG CGAGCCCCAA CTGCGTGAAA CCAAAAATAT TTTTTGATAC TGATGATGAT
    GATGATATGC CACATAGTAC TTCCAGGTGG AGGGATACAG CCAACGCTGA AGGCCACTCG
    GATGGCCTAG CAAGAAGAGC CAGAGGTGAG AGTTCAGGTG GCTACTCTGA GCTGAAGTAC
    CCCGAAGACA AGAGGGAAGC CAGGAATGAC CAAGTGAAGC CAGAAAAGGT ACCAAGACGG
    CGGCGAACCA TGGCTGACCC TGACTTCTGG ACGTACAGCG ACGATTACTA CAAATACTGT
    GATGAAGACT CTGACAGTGA CAAAGAGTGG ACTGCTGCTC TGCGGCGGAA GTATCGAGGT
    CGAGAGCAGA ATCTGTCGTC CAGTGGTGAA AGCTGGGAGA CTCTGCCAGG GAAAGAGGAG
    CACGACTCTG AGCAGGTCAG AGTGAATGCT GGCGCCGGCG CCAGTGCCAG CCCGGGTACC
    GGCGCCAGCG CCGGCAGCAA CAGCAGCAGT GAACTGGAAG ACCTCCGAGG ACCACCTCTT
    CAGGAAGAGG AACACGCATC CCCCAGAGAA CGAGAAGCTC CTTGGCTCCA GTACAACGAA
    AATGAGAGTA GCAGTGAGGG GGATAATGAA TCTGCTCAGG AGTATCTACA GCCTGGGGTG
    TTCATGCTCG ATGGAAACAA CAATCTTGAA GATGACTCCA GCGTGAGTGA AGACCTAGAA
    GTGGATTGGA GCCTCTTTGA TGGGTTCGCA GATGGCTTGG GGGTGGCCGA AGCCATTTCC
    TACGTGGATC CTCAGTTTCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG
    GAAACTGCCC TGGCGCACCT AGAGTCTCTC GCAGTGGACG TGGAGGTGGC CAACCCACCG
    GCGAGCAAGG AGAGCATCGA CACTCTCCCT GAGATCCTGG TCACTGAAGA TCACAGTGCA
    GTGGGGCAGG AAATGTGTTG CCCCATCTGT TGTAGTGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTGC CTTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAA
    TCAGGCACCT GCCCTGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32479
    Clone ID Related Accession (Same CDS sequence) XM_005641441.2 , XM_005641441.3 , XM_014111872.1 , XM_014111872.2
    Accession Version XM_005641441.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGGTACCCA
    CCGAGAGAGT ACAGGGCTTC AGGTAGCAGA AGAGGATTGG CTTATGGACA TGTTGACTCT
    TTTGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGAGC ACCAGTTAGA
    GGGAAAACTG GCAAGTTTAA AGATGATAAG CCCTACGACC CAGAGAAAGG GGCCAGGTCT
    TTGGCTGGGG TGGCTCCACA GTTCTCTAGC TTTAAGCGTG ATGTGAGAGA GGAGCTTGAC
    AAGTTAGACC CGGCCCCTGC AGCAAGATGC TCTGTTAGCA GAGCTGAGTT CCTGCAGCAA
    AATAGCATGG CCTCTCAGAC ATCTGCTGAA GGCAAGGTGG CTACCCGTGG TGACAGCCGG
    GAGAGTCAGA GACGGGAGCA GAATTTCCCT GCACATCCCA GCAGGGATCC TGTGAGTATT
    TGTGGTGGTG GGGAAAACAC CCCCAAGAGT GCAGAGGAGC CGGTGGTGAG GCCTAAAATC
    AGAAATCTGG CGAGCCCCAA CTGCGTGAAA CCAAAAATAT TTTTTGATAC TGATGATGAT
    GATGATATGC CACATAGTAC TTCCAGGTGG AGGGATACAG CCAACGCTGA AGGCCACTCG
    GATGGCCTAG CAAGAAGAGC CAGAGGTGAG AGTTCAGGTG GCTACTCTGA GCTGAAGTAC
    CCCGAAGACA AGAGGGAAGC CAGGAATGAC CAAGTGAAGC CAGAAAAGGT ACCAAGACGG
    CGGCGAACCA TGGCTGACCC TGACTTCTGG ACGTACAGCG ACGATTACTA CAAATACTGT
    GATGAAGACT CTGACAGTGA CAAAGAGTGG ACTGCTGCTC TGCGGCGGAA GTATCGAGGT
    CGAGAGCAGA ATCTGTCGTC CAGTGGTGAA AGCTGGGAGA CTCTGCCAGG GAAAGAGGAG
    CACGACTCTG AGCAGGTCAG AGTGAATGCT GGCGCCGGCG CCAGTGCCAG CCCGGGTACC
    GGCGCCAGCG CCGGCAGCAA CAGCAGCAGT GAACTGGAAG ACCTCCGAGG ACCACCTCTT
    CAGGAAGAGG AACACGCATC CCCCAGAGAA CGAGAAGCTC CTTGGCTCCA GTACAACGAA
    AATGAGAGTA GCAGTGAGGG GGATAATGAA TCTGCTCAGG AGTATCTACA GCCTGGGGTG
    TTCATGCTCG ATGGAAACAA CAATCTTGAA GATGACTCCA GCGTGAGTGA AGACCTAGAA
    GTGGATTGGA GCCTCTTTGA TGGGTTCGCA GATGGCTTGG GGGTGGCCGA AGCCATTTCC
    TACGTGGATC CTCAGTTTCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG
    GAAACTGCCC TGGCGCACCT AGAGTCTCTC GCAGTGGACG TGGAGGTGGC CAACCCACCG
    GCGAGCAAGG AGAGCATCGA CACTCTCCCT GAGATCCTGG TCACTGAAGA TCACAGTGCA
    GTGGGGCAGG AAATGTGTTG CCCCATCTGT TGTAGTGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTGC CTTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAA
    TCAGGCACCT GCCCTGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641498.1
    CDS209..2116
    Translation

    Target ORF information:

    RefSeq Version XM_005641441.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGGTACCCA
    CCGAGAGAGT ACAGGGCTTC AGGTAGCAGA AGAGGATTGG CTTATGGACA TGTTGACTCT
    TTTGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGAGC ACCAGTTAGA
    GGGAAAACTG GCAAGTTTAA AGATGATAAG CCCTACGACC CAGAGAAAGG GGCCAGGTCT
    TTGGCTGGGG TGGCTCCACA GTTCTCTAGC TTTAAGCGTG ATGTGAGAGA GGAGCTTGAC
    AAGTTAGACC CGGCCCCTGC AGCAAGATGC TCTGTTAGCA GAGCTGAGTT CCTGCAGCAA
    AATAGCATGG CCTCTCAGAC ATCTGCTGAA GGCAAGGTGG CTACCCGTGG TGACAGCCGG
    GAGAGTCAGA GACGGGAGCA GAATTTCCCT GCACATCCCA GCAGGGATCC TGTGAGTATT
    TGTGGTGGTG GGGAAAACAC CCCCAAGAGT GCAGAGGAGC CGGTGGTGAG GCCTAAAATC
    AGAAATCTGG CGAGCCCCAA CTGCGTGAAA CCAAAAATAT TTTTTGATAC TGATGATGAT
    GATGATATGC CACATAGTAC TTCCAGGTGG AGGGATACAG CCAACGCTGA AGGCCACTCG
    GATGGCCTAG CAAGAAGAGC CAGAGGTGAG AGTTCAGGTG GCTACTCTGA GCTGAAGTAC
    CCCGAAGACA AGAGGGAAGC CAGGAATGAC CAAGTGAAGC CAGAAAAGGT ACCAAGACGG
    CGGCGAACCA TGGCTGACCC TGACTTCTGG ACGTACAGCG ACGATTACTA CAAATACTGT
    GATGAAGACT CTGACAGTGA CAAAGAGTGG ACTGCTGCTC TGCGGCGGAA GTATCGAGGT
    CGAGAGCAGA ATCTGTCGTC CAGTGGTGAA AGCTGGGAGA CTCTGCCAGG GAAAGAGGAG
    CACGACTCTG AGCAGGTCAG AGTGAATGCT GGCGCCGGCG CCAGTGCCAG CCCGGGTACC
    GGCGCCAGCG CCGGCAGCAA CAGCAGCAGT GAACTGGAAG ACCTCCGAGG ACCACCTCTT
    CAGGAAGAGG AACACGCATC CCCCAGAGAA CGAGAAGCTC CTTGGCTCCA GTACAACGAA
    AATGAGAGTA GCAGTGAGGG GGATAATGAA TCTGCTCAGG AGTATCTACA GCCTGGGGTG
    TTCATGCTCG ATGGAAACAA CAATCTTGAA GATGACTCCA GCGTGAGTGA AGACCTAGAA
    GTGGATTGGA GCCTCTTTGA TGGGTTCGCA GATGGCTTGG GGGTGGCCGA AGCCATTTCC
    TACGTGGATC CTCAGTTTCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG
    GAAACTGCCC TGGCGCACCT AGAGTCTCTC GCAGTGGACG TGGAGGTGGC CAACCCACCG
    GCGAGCAAGG AGAGCATCGA CACTCTCCCT GAGATCCTGG TCACTGAAGA TCACAGTGCA
    GTGGGGCAGG AAATGTGTTG CCCCATCTGT TGTAGTGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTGC CTTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAA
    TCAGGCACCT GCCCTGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32479
    Clone ID Related Accession (Same CDS sequence) XM_005641441.2 , XM_005641441.3 , XM_014111872.1 , XM_014111872.2
    Accession Version XM_014111872.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGGTACCCA
    CCGAGAGAGT ACAGGGCTTC AGGTAGCAGA AGAGGATTGG CTTATGGACA TGTTGACTCT
    TTTGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGAGC ACCAGTTAGA
    GGGAAAACTG GCAAGTTTAA AGATGATAAG CCCTACGACC CAGAGAAAGG GGCCAGGTCT
    TTGGCTGGGG TGGCTCCACA GTTCTCTAGC TTTAAGCGTG ATGTGAGAGA GGAGCTTGAC
    AAGTTAGACC CGGCCCCTGC AGCAAGATGC TCTGTTAGCA GAGCTGAGTT CCTGCAGCAA
    AATAGCATGG CCTCTCAGAC ATCTGCTGAA GGCAAGGTGG CTACCCGTGG TGACAGCCGG
    GAGAGTCAGA GACGGGAGCA GAATTTCCCT GCACATCCCA GCAGGGATCC TGTGAGTATT
    TGTGGTGGTG GGGAAAACAC CCCCAAGAGT GCAGAGGAGC CGGTGGTGAG GCCTAAAATC
    AGAAATCTGG CGAGCCCCAA CTGCGTGAAA CCAAAAATAT TTTTTGATAC TGATGATGAT
    GATGATATGC CACATAGTAC TTCCAGGTGG AGGGATACAG CCAACGCTGA AGGCCACTCG
    GATGGCCTAG CAAGAAGAGC CAGAGGTGAG AGTTCAGGTG GCTACTCTGA GCTGAAGTAC
    CCCGAAGACA AGAGGGAAGC CAGGAATGAC CAAGTGAAGC CAGAAAAGGT ACCAAGACGG
    CGGCGAACCA TGGCTGACCC TGACTTCTGG ACGTACAGCG ACGATTACTA CAAATACTGT
    GATGAAGACT CTGACAGTGA CAAAGAGTGG ACTGCTGCTC TGCGGCGGAA GTATCGAGGT
    CGAGAGCAGA ATCTGTCGTC CAGTGGTGAA AGCTGGGAGA CTCTGCCAGG GAAAGAGGAG
    CACGACTCTG AGCAGGTCAG AGTGAATGCT GGCGCCGGCG CCAGTGCCAG CCCGGGTACC
    GGCGCCAGCG CCGGCAGCAA CAGCAGCAGT GAACTGGAAG ACCTCCGAGG ACCACCTCTT
    CAGGAAGAGG AACACGCATC CCCCAGAGAA CGAGAAGCTC CTTGGCTCCA GTACAACGAA
    AATGAGAGTA GCAGTGAGGG GGATAATGAA TCTGCTCAGG AGTATCTACA GCCTGGGGTG
    TTCATGCTCG ATGGAAACAA CAATCTTGAA GATGACTCCA GCGTGAGTGA AGACCTAGAA
    GTGGATTGGA GCCTCTTTGA TGGGTTCGCA GATGGCTTGG GGGTGGCCGA AGCCATTTCC
    TACGTGGATC CTCAGTTTCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG
    GAAACTGCCC TGGCGCACCT AGAGTCTCTC GCAGTGGACG TGGAGGTGGC CAACCCACCG
    GCGAGCAAGG AGAGCATCGA CACTCTCCCT GAGATCCTGG TCACTGAAGA TCACAGTGCA
    GTGGGGCAGG AAATGTGTTG CCCCATCTGT TGTAGTGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTGC CTTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAA
    TCAGGCACCT GCCCTGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967347.1
    CDS378..2285
    Translation

    Target ORF information:

    RefSeq Version XM_014111872.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGGTACCCA
    CCGAGAGAGT ACAGGGCTTC AGGTAGCAGA AGAGGATTGG CTTATGGACA TGTTGACTCT
    TTTGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGAGC ACCAGTTAGA
    GGGAAAACTG GCAAGTTTAA AGATGATAAG CCCTACGACC CAGAGAAAGG GGCCAGGTCT
    TTGGCTGGGG TGGCTCCACA GTTCTCTAGC TTTAAGCGTG ATGTGAGAGA GGAGCTTGAC
    AAGTTAGACC CGGCCCCTGC AGCAAGATGC TCTGTTAGCA GAGCTGAGTT CCTGCAGCAA
    AATAGCATGG CCTCTCAGAC ATCTGCTGAA GGCAAGGTGG CTACCCGTGG TGACAGCCGG
    GAGAGTCAGA GACGGGAGCA GAATTTCCCT GCACATCCCA GCAGGGATCC TGTGAGTATT
    TGTGGTGGTG GGGAAAACAC CCCCAAGAGT GCAGAGGAGC CGGTGGTGAG GCCTAAAATC
    AGAAATCTGG CGAGCCCCAA CTGCGTGAAA CCAAAAATAT TTTTTGATAC TGATGATGAT
    GATGATATGC CACATAGTAC TTCCAGGTGG AGGGATACAG CCAACGCTGA AGGCCACTCG
    GATGGCCTAG CAAGAAGAGC CAGAGGTGAG AGTTCAGGTG GCTACTCTGA GCTGAAGTAC
    CCCGAAGACA AGAGGGAAGC CAGGAATGAC CAAGTGAAGC CAGAAAAGGT ACCAAGACGG
    CGGCGAACCA TGGCTGACCC TGACTTCTGG ACGTACAGCG ACGATTACTA CAAATACTGT
    GATGAAGACT CTGACAGTGA CAAAGAGTGG ACTGCTGCTC TGCGGCGGAA GTATCGAGGT
    CGAGAGCAGA ATCTGTCGTC CAGTGGTGAA AGCTGGGAGA CTCTGCCAGG GAAAGAGGAG
    CACGACTCTG AGCAGGTCAG AGTGAATGCT GGCGCCGGCG CCAGTGCCAG CCCGGGTACC
    GGCGCCAGCG CCGGCAGCAA CAGCAGCAGT GAACTGGAAG ACCTCCGAGG ACCACCTCTT
    CAGGAAGAGG AACACGCATC CCCCAGAGAA CGAGAAGCTC CTTGGCTCCA GTACAACGAA
    AATGAGAGTA GCAGTGAGGG GGATAATGAA TCTGCTCAGG AGTATCTACA GCCTGGGGTG
    TTCATGCTCG ATGGAAACAA CAATCTTGAA GATGACTCCA GCGTGAGTGA AGACCTAGAA
    GTGGATTGGA GCCTCTTTGA TGGGTTCGCA GATGGCTTGG GGGTGGCCGA AGCCATTTCC
    TACGTGGATC CTCAGTTTCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG
    GAAACTGCCC TGGCGCACCT AGAGTCTCTC GCAGTGGACG TGGAGGTGGC CAACCCACCG
    GCGAGCAAGG AGAGCATCGA CACTCTCCCT GAGATCCTGG TCACTGAAGA TCACAGTGCA
    GTGGGGCAGG AAATGTGTTG CCCCATCTGT TGTAGTGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTGC CTTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAA
    TCAGGCACCT GCCCTGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32479
    Clone ID Related Accession (Same CDS sequence) XM_005641441.2 , XM_005641441.3 , XM_014111872.1 , XM_014111872.2
    Accession Version XM_014111872.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1908bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGGTACCCA
    CCGAGAGAGT ACAGGGCTTC AGGTAGCAGA AGAGGATTGG CTTATGGACA TGTTGACTCT
    TTTGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGAGC ACCAGTTAGA
    GGGAAAACTG GCAAGTTTAA AGATGATAAG CCCTACGACC CAGAGAAAGG GGCCAGGTCT
    TTGGCTGGGG TGGCTCCACA GTTCTCTAGC TTTAAGCGTG ATGTGAGAGA GGAGCTTGAC
    AAGTTAGACC CGGCCCCTGC AGCAAGATGC TCTGTTAGCA GAGCTGAGTT CCTGCAGCAA
    AATAGCATGG CCTCTCAGAC ATCTGCTGAA GGCAAGGTGG CTACCCGTGG TGACAGCCGG
    GAGAGTCAGA GACGGGAGCA GAATTTCCCT GCACATCCCA GCAGGGATCC TGTGAGTATT
    TGTGGTGGTG GGGAAAACAC CCCCAAGAGT GCAGAGGAGC CGGTGGTGAG GCCTAAAATC
    AGAAATCTGG CGAGCCCCAA CTGCGTGAAA CCAAAAATAT TTTTTGATAC TGATGATGAT
    GATGATATGC CACATAGTAC TTCCAGGTGG AGGGATACAG CCAACGCTGA AGGCCACTCG
    GATGGCCTAG CAAGAAGAGC CAGAGGTGAG AGTTCAGGTG GCTACTCTGA GCTGAAGTAC
    CCCGAAGACA AGAGGGAAGC CAGGAATGAC CAAGTGAAGC CAGAAAAGGT ACCAAGACGG
    CGGCGAACCA TGGCTGACCC TGACTTCTGG ACGTACAGCG ACGATTACTA CAAATACTGT
    GATGAAGACT CTGACAGTGA CAAAGAGTGG ACTGCTGCTC TGCGGCGGAA GTATCGAGGT
    CGAGAGCAGA ATCTGTCGTC CAGTGGTGAA AGCTGGGAGA CTCTGCCAGG GAAAGAGGAG
    CACGACTCTG AGCAGGTCAG AGTGAATGCT GGCGCCGGCG CCAGTGCCAG CCCGGGTACC
    GGCGCCAGCG CCGGCAGCAA CAGCAGCAGT GAACTGGAAG ACCTCCGAGG ACCACCTCTT
    CAGGAAGAGG AACACGCATC CCCCAGAGAA CGAGAAGCTC CTTGGCTCCA GTACAACGAA
    AATGAGAGTA GCAGTGAGGG GGATAATGAA TCTGCTCAGG AGTATCTACA GCCTGGGGTG
    TTCATGCTCG ATGGAAACAA CAATCTTGAA GATGACTCCA GCGTGAGTGA AGACCTAGAA
    GTGGATTGGA GCCTCTTTGA TGGGTTCGCA GATGGCTTGG GGGTGGCCGA AGCCATTTCC
    TACGTGGATC CTCAGTTTCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG
    GAAACTGCCC TGGCGCACCT AGAGTCTCTC GCAGTGGACG TGGAGGTGGC CAACCCACCG
    GCGAGCAAGG AGAGCATCGA CACTCTCCCT GAGATCCTGG TCACTGAAGA TCACAGTGCA
    GTGGGGCAGG AAATGTGTTG CCCCATCTGT TGTAGTGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTGC CTTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAA
    TCAGGCACCT GCCCTGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967347.1
    CDS372..2279
    Translation

    Target ORF information:

    RefSeq Version XM_014111872.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111872.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGGTACCCA
    CCGAGAGAGT ACAGGGCTTC AGGTAGCAGA AGAGGATTGG CTTATGGACA TGTTGACTCT
    TTTGGGGCAG ATGATAGTGA GGAGGAGGGG GCTGGGCCTG TTGAGCGAGC ACCAGTTAGA
    GGGAAAACTG GCAAGTTTAA AGATGATAAG CCCTACGACC CAGAGAAAGG GGCCAGGTCT
    TTGGCTGGGG TGGCTCCACA GTTCTCTAGC TTTAAGCGTG ATGTGAGAGA GGAGCTTGAC
    AAGTTAGACC CGGCCCCTGC AGCAAGATGC TCTGTTAGCA GAGCTGAGTT CCTGCAGCAA
    AATAGCATGG CCTCTCAGAC ATCTGCTGAA GGCAAGGTGG CTACCCGTGG TGACAGCCGG
    GAGAGTCAGA GACGGGAGCA GAATTTCCCT GCACATCCCA GCAGGGATCC TGTGAGTATT
    TGTGGTGGTG GGGAAAACAC CCCCAAGAGT GCAGAGGAGC CGGTGGTGAG GCCTAAAATC
    AGAAATCTGG CGAGCCCCAA CTGCGTGAAA CCAAAAATAT TTTTTGATAC TGATGATGAT
    GATGATATGC CACATAGTAC TTCCAGGTGG AGGGATACAG CCAACGCTGA AGGCCACTCG
    GATGGCCTAG CAAGAAGAGC CAGAGGTGAG AGTTCAGGTG GCTACTCTGA GCTGAAGTAC
    CCCGAAGACA AGAGGGAAGC CAGGAATGAC CAAGTGAAGC CAGAAAAGGT ACCAAGACGG
    CGGCGAACCA TGGCTGACCC TGACTTCTGG ACGTACAGCG ACGATTACTA CAAATACTGT
    GATGAAGACT CTGACAGTGA CAAAGAGTGG ACTGCTGCTC TGCGGCGGAA GTATCGAGGT
    CGAGAGCAGA ATCTGTCGTC CAGTGGTGAA AGCTGGGAGA CTCTGCCAGG GAAAGAGGAG
    CACGACTCTG AGCAGGTCAG AGTGAATGCT GGCGCCGGCG CCAGTGCCAG CCCGGGTACC
    GGCGCCAGCG CCGGCAGCAA CAGCAGCAGT GAACTGGAAG ACCTCCGAGG ACCACCTCTT
    CAGGAAGAGG AACACGCATC CCCCAGAGAA CGAGAAGCTC CTTGGCTCCA GTACAACGAA
    AATGAGAGTA GCAGTGAGGG GGATAATGAA TCTGCTCAGG AGTATCTACA GCCTGGGGTG
    TTCATGCTCG ATGGAAACAA CAATCTTGAA GATGACTCCA GCGTGAGTGA AGACCTAGAA
    GTGGATTGGA GCCTCTTTGA TGGGTTCGCA GATGGCTTGG GGGTGGCCGA AGCCATTTCC
    TACGTGGATC CTCAGTTTCT CACCTACATG GCACTTGAAG AACGCCTGGC CCAGGCAATG
    GAAACTGCCC TGGCGCACCT AGAGTCTCTC GCAGTGGACG TGGAGGTGGC CAACCCACCG
    GCGAGCAAGG AGAGCATCGA CACTCTCCCT GAGATCCTGG TCACTGAAGA TCACAGTGCA
    GTGGGGCAGG AAATGTGTTG CCCCATCTGT TGTAGTGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTGC CTTGCCACCA CTATTTCCAC AAGCCGTGTG TGTCCATCTG GCTTCAGAAA
    TCAGGCACCT GCCCTGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32480
    Clone ID Related Accession (Same CDS sequence) XM_005641442.2 , XM_005641442.3
    Accession Version XM_005641442.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GGTCACCATT TTCCATCACC 
    CGTCGTAGTT GGGAGGACAG TGAGAGCTCT GGAGCCAGCC TGAATGTTGA TAATGAGGAC
    TACTCCAGGT ACCCACCGAG AGAGTACAGG GCTTCAGGTA GCAGAAGAGG ATTGGCTTAT
    GGACATGTTG ACTCTTTTGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGCACCAG TTAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCCCTA CGACCCAGAG
    AAAGGGGCCA GGTCTTTGGC TGGGGTGGCT CCACAGTTCT CTAGCTTTAA GCGTGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCGGCC CCTGCAGCAA GATGCTCTGT TAGCAGAGCT
    GAGTTCCTGC AGCAAAATAG CATGGCCTCT CAGACATCTG CTGAAGGCAA GGTGGCTACC
    CGTGGTGACA GCCGGGAGAG TCAGAGACGG GAGCAGAATT TCCCTGCACA TCCCAGCAGG
    GATCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCCCCA AGAGTGCAGA GGAGCCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCGAGC CCCAACTGCG TGAAACCAAA AATATTTTTT
    GATACTGATG ATGATGATGA TATGCCACAT AGTACTTCCA GGTGGAGGGA TACAGCCAAC
    GCTGAAGGCC ACTCGGATGG CCTAGCAAGA AGAGCCAGAG GTGAGAGTTC AGGTGGCTAC
    TCTGAGCTGA AGTACCCCGA AGACAAGAGG GAAGCCAGGA ATGACCAAGT GAAGCCAGAA
    AAGGTACCAA GACGGCGGCG AACCATGGCT GACCCTGACT TCTGGACGTA CAGCGACGAT
    TACTACAAAT ACTGTGATGA AGACTCTGAC AGTGACAAAG AGTGGACTGC TGCTCTGCGG
    CGGAAGTATC GAGGTCGAGA GCAGAATCTG TCGTCCAGTG GTGAAAGCTG GGAGACTCTG
    CCAGGGAAAG AGGAGCACGA CTCTGAGCAG GTCAGAGTGA ATGCTGGCGC CGGCGCCAGT
    GCCAGCCCGG GTACCGGCGC CAGCGCCGGC AGCAACAGCA GCAGTGAACT GGAAGACCTC
    CGAGGACCAC CTCTTCAGGA AGAGGAACAC GCATCCCCCA GAGAACGAGA AGCTCCTTGG
    CTCCAGTACA ACGAAAATGA GAGTAGCAGT GAGGGGGATA ATGAATCTGC TCAGGAGTAT
    CTACAGCCTG GGGTGTTCAT GCTCGATGGA AACAACAATC TTGAAGATGA CTCCAGCGTG
    AGTGAAGACC TAGAAGTGGA TTGGAGCCTC TTTGATGGGT TCGCAGATGG CTTGGGGGTG
    GCCGAAGCCA TTTCCTACGT GGATCCTCAG TTTCTCACCT ACATGGCACT TGAAGAACGC
    CTGGCCCAGG CAATGGAAAC TGCCCTGGCG CACCTAGAGT CTCTCGCAGT GGACGTGGAG
    GTGGCCAACC CACCGGCGAG CAAGGAGAGC ATCGACACTC TCCCTGAGAT CCTGGTCACT
    GAAGATCACA GTGCAGTGGG GCAGGAAATG TGTTGCCCCA TCTGTTGTAG TGAATATGTG
    AAGGGGGAGG TGGCAACTGA GCTGCCTTGC CACCACTATT TCCACAAGCC GTGTGTGTCC
    ATCTGGCTTC AGAAATCAGG CACCTGCCCT GTGTGCCGCT GCATGTTCCC TCCCCCGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641499.1
    CDS218..1960
    Translation

    Target ORF information:

    RefSeq Version XM_005641442.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641442.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GGTCACCATT TTCCATCACC 
    CGTCGTAGTT GGGAGGACAG TGAGAGCTCT GGAGCCAGCC TGAATGTTGA TAATGAGGAC
    TACTCCAGGT ACCCACCGAG AGAGTACAGG GCTTCAGGTA GCAGAAGAGG ATTGGCTTAT
    GGACATGTTG ACTCTTTTGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGCACCAG TTAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCCCTA CGACCCAGAG
    AAAGGGGCCA GGTCTTTGGC TGGGGTGGCT CCACAGTTCT CTAGCTTTAA GCGTGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCGGCC CCTGCAGCAA GATGCTCTGT TAGCAGAGCT
    GAGTTCCTGC AGCAAAATAG CATGGCCTCT CAGACATCTG CTGAAGGCAA GGTGGCTACC
    CGTGGTGACA GCCGGGAGAG TCAGAGACGG GAGCAGAATT TCCCTGCACA TCCCAGCAGG
    GATCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCCCCA AGAGTGCAGA GGAGCCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCGAGC CCCAACTGCG TGAAACCAAA AATATTTTTT
    GATACTGATG ATGATGATGA TATGCCACAT AGTACTTCCA GGTGGAGGGA TACAGCCAAC
    GCTGAAGGCC ACTCGGATGG CCTAGCAAGA AGAGCCAGAG GTGAGAGTTC AGGTGGCTAC
    TCTGAGCTGA AGTACCCCGA AGACAAGAGG GAAGCCAGGA ATGACCAAGT GAAGCCAGAA
    AAGGTACCAA GACGGCGGCG AACCATGGCT GACCCTGACT TCTGGACGTA CAGCGACGAT
    TACTACAAAT ACTGTGATGA AGACTCTGAC AGTGACAAAG AGTGGACTGC TGCTCTGCGG
    CGGAAGTATC GAGGTCGAGA GCAGAATCTG TCGTCCAGTG GTGAAAGCTG GGAGACTCTG
    CCAGGGAAAG AGGAGCACGA CTCTGAGCAG GTCAGAGTGA ATGCTGGCGC CGGCGCCAGT
    GCCAGCCCGG GTACCGGCGC CAGCGCCGGC AGCAACAGCA GCAGTGAACT GGAAGACCTC
    CGAGGACCAC CTCTTCAGGA AGAGGAACAC GCATCCCCCA GAGAACGAGA AGCTCCTTGG
    CTCCAGTACA ACGAAAATGA GAGTAGCAGT GAGGGGGATA ATGAATCTGC TCAGGAGTAT
    CTACAGCCTG GGGTGTTCAT GCTCGATGGA AACAACAATC TTGAAGATGA CTCCAGCGTG
    AGTGAAGACC TAGAAGTGGA TTGGAGCCTC TTTGATGGGT TCGCAGATGG CTTGGGGGTG
    GCCGAAGCCA TTTCCTACGT GGATCCTCAG TTTCTCACCT ACATGGCACT TGAAGAACGC
    CTGGCCCAGG CAATGGAAAC TGCCCTGGCG CACCTAGAGT CTCTCGCAGT GGACGTGGAG
    GTGGCCAACC CACCGGCGAG CAAGGAGAGC ATCGACACTC TCCCTGAGAT CCTGGTCACT
    GAAGATCACA GTGCAGTGGG GCAGGAAATG TGTTGCCCCA TCTGTTGTAG TGAATATGTG
    AAGGGGGAGG TGGCAACTGA GCTGCCTTGC CACCACTATT TCCACAAGCC GTGTGTGTCC
    ATCTGGCTTC AGAAATCAGG CACCTGCCCT GTGTGCCGCT GCATGTTCCC TCCCCCGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32480
    Clone ID Related Accession (Same CDS sequence) XM_005641442.2 , XM_005641442.3
    Accession Version XM_005641442.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GGTCACCATT TTCCATCACC 
    CGTCGTAGTT GGGAGGACAG TGAGAGCTCT GGAGCCAGCC TGAATGTTGA TAATGAGGAC
    TACTCCAGGT ACCCACCGAG AGAGTACAGG GCTTCAGGTA GCAGAAGAGG ATTGGCTTAT
    GGACATGTTG ACTCTTTTGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGCACCAG TTAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCCCTA CGACCCAGAG
    AAAGGGGCCA GGTCTTTGGC TGGGGTGGCT CCACAGTTCT CTAGCTTTAA GCGTGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCGGCC CCTGCAGCAA GATGCTCTGT TAGCAGAGCT
    GAGTTCCTGC AGCAAAATAG CATGGCCTCT CAGACATCTG CTGAAGGCAA GGTGGCTACC
    CGTGGTGACA GCCGGGAGAG TCAGAGACGG GAGCAGAATT TCCCTGCACA TCCCAGCAGG
    GATCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCCCCA AGAGTGCAGA GGAGCCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCGAGC CCCAACTGCG TGAAACCAAA AATATTTTTT
    GATACTGATG ATGATGATGA TATGCCACAT AGTACTTCCA GGTGGAGGGA TACAGCCAAC
    GCTGAAGGCC ACTCGGATGG CCTAGCAAGA AGAGCCAGAG GTGAGAGTTC AGGTGGCTAC
    TCTGAGCTGA AGTACCCCGA AGACAAGAGG GAAGCCAGGA ATGACCAAGT GAAGCCAGAA
    AAGGTACCAA GACGGCGGCG AACCATGGCT GACCCTGACT TCTGGACGTA CAGCGACGAT
    TACTACAAAT ACTGTGATGA AGACTCTGAC AGTGACAAAG AGTGGACTGC TGCTCTGCGG
    CGGAAGTATC GAGGTCGAGA GCAGAATCTG TCGTCCAGTG GTGAAAGCTG GGAGACTCTG
    CCAGGGAAAG AGGAGCACGA CTCTGAGCAG GTCAGAGTGA ATGCTGGCGC CGGCGCCAGT
    GCCAGCCCGG GTACCGGCGC CAGCGCCGGC AGCAACAGCA GCAGTGAACT GGAAGACCTC
    CGAGGACCAC CTCTTCAGGA AGAGGAACAC GCATCCCCCA GAGAACGAGA AGCTCCTTGG
    CTCCAGTACA ACGAAAATGA GAGTAGCAGT GAGGGGGATA ATGAATCTGC TCAGGAGTAT
    CTACAGCCTG GGGTGTTCAT GCTCGATGGA AACAACAATC TTGAAGATGA CTCCAGCGTG
    AGTGAAGACC TAGAAGTGGA TTGGAGCCTC TTTGATGGGT TCGCAGATGG CTTGGGGGTG
    GCCGAAGCCA TTTCCTACGT GGATCCTCAG TTTCTCACCT ACATGGCACT TGAAGAACGC
    CTGGCCCAGG CAATGGAAAC TGCCCTGGCG CACCTAGAGT CTCTCGCAGT GGACGTGGAG
    GTGGCCAACC CACCGGCGAG CAAGGAGAGC ATCGACACTC TCCCTGAGAT CCTGGTCACT
    GAAGATCACA GTGCAGTGGG GCAGGAAATG TGTTGCCCCA TCTGTTGTAG TGAATATGTG
    AAGGGGGAGG TGGCAACTGA GCTGCCTTGC CACCACTATT TCCACAAGCC GTGTGTGTCC
    ATCTGGCTTC AGAAATCAGG CACCTGCCCT GTGTGCCGCT GCATGTTCCC TCCCCCGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641499.1
    CDS225..1967
    Translation

    Target ORF information:

    RefSeq Version XM_005641442.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641442.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCACAGAT CAGCCCCCAG TCAAACCACC AAGAGGAGCC GGTCACCATT TTCCATCACC 
    CGTCGTAGTT GGGAGGACAG TGAGAGCTCT GGAGCCAGCC TGAATGTTGA TAATGAGGAC
    TACTCCAGGT ACCCACCGAG AGAGTACAGG GCTTCAGGTA GCAGAAGAGG ATTGGCTTAT
    GGACATGTTG ACTCTTTTGG GGCAGATGAT AGTGAGGAGG AGGGGGCTGG GCCTGTTGAG
    CGAGCACCAG TTAGAGGGAA AACTGGCAAG TTTAAAGATG ATAAGCCCTA CGACCCAGAG
    AAAGGGGCCA GGTCTTTGGC TGGGGTGGCT CCACAGTTCT CTAGCTTTAA GCGTGATGTG
    AGAGAGGAGC TTGACAAGTT AGACCCGGCC CCTGCAGCAA GATGCTCTGT TAGCAGAGCT
    GAGTTCCTGC AGCAAAATAG CATGGCCTCT CAGACATCTG CTGAAGGCAA GGTGGCTACC
    CGTGGTGACA GCCGGGAGAG TCAGAGACGG GAGCAGAATT TCCCTGCACA TCCCAGCAGG
    GATCCTGTGA GTATTTGTGG TGGTGGGGAA AACACCCCCA AGAGTGCAGA GGAGCCGGTG
    GTGAGGCCTA AAATCAGAAA TCTGGCGAGC CCCAACTGCG TGAAACCAAA AATATTTTTT
    GATACTGATG ATGATGATGA TATGCCACAT AGTACTTCCA GGTGGAGGGA TACAGCCAAC
    GCTGAAGGCC ACTCGGATGG CCTAGCAAGA AGAGCCAGAG GTGAGAGTTC AGGTGGCTAC
    TCTGAGCTGA AGTACCCCGA AGACAAGAGG GAAGCCAGGA ATGACCAAGT GAAGCCAGAA
    AAGGTACCAA GACGGCGGCG AACCATGGCT GACCCTGACT TCTGGACGTA CAGCGACGAT
    TACTACAAAT ACTGTGATGA AGACTCTGAC AGTGACAAAG AGTGGACTGC TGCTCTGCGG
    CGGAAGTATC GAGGTCGAGA GCAGAATCTG TCGTCCAGTG GTGAAAGCTG GGAGACTCTG
    CCAGGGAAAG AGGAGCACGA CTCTGAGCAG GTCAGAGTGA ATGCTGGCGC CGGCGCCAGT
    GCCAGCCCGG GTACCGGCGC CAGCGCCGGC AGCAACAGCA GCAGTGAACT GGAAGACCTC
    CGAGGACCAC CTCTTCAGGA AGAGGAACAC GCATCCCCCA GAGAACGAGA AGCTCCTTGG
    CTCCAGTACA ACGAAAATGA GAGTAGCAGT GAGGGGGATA ATGAATCTGC TCAGGAGTAT
    CTACAGCCTG GGGTGTTCAT GCTCGATGGA AACAACAATC TTGAAGATGA CTCCAGCGTG
    AGTGAAGACC TAGAAGTGGA TTGGAGCCTC TTTGATGGGT TCGCAGATGG CTTGGGGGTG
    GCCGAAGCCA TTTCCTACGT GGATCCTCAG TTTCTCACCT ACATGGCACT TGAAGAACGC
    CTGGCCCAGG CAATGGAAAC TGCCCTGGCG CACCTAGAGT CTCTCGCAGT GGACGTGGAG
    GTGGCCAACC CACCGGCGAG CAAGGAGAGC ATCGACACTC TCCCTGAGAT CCTGGTCACT
    GAAGATCACA GTGCAGTGGG GCAGGAAATG TGTTGCCCCA TCTGTTGTAG TGAATATGTG
    AAGGGGGAGG TGGCAACTGA GCTGCCTTGC CACCACTATT TCCACAAGCC GTGTGTGTCC
    ATCTGGCTTC AGAAATCAGG CACCTGCCCT GTGTGCCGCT GCATGTTCCC TCCCCCGCTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32481
    Clone ID Related Accession (Same CDS sequence) XM_014111873.2 , XM_014111873.1
    Accession Version XM_014111873.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGTACTTCC
    AGGTGGAGGG ATACAGCCAA CGCTGAAGGC CACTCGGATG GCCTAGCAAG AAGAGCCAGA
    GGTGAGAGTT CAGGTGGCTA CTCTGAGCTG AAGTACCCCG AAGACAAGAG GGAAGCCAGG
    AATGACCAAG TGAAGCCAGA AAAGGTACCA AGACGGCGGC GAACCATGGC TGACCCTGAC
    TTCTGGACGT ACAGCGACGA TTACTACAAA TACTGTGATG AAGACTCTGA CAGTGACAAA
    GAGTGGACTG CTGCTCTGCG GCGGAAGTAT CGAGGTCGAG AGCAGAATCT GTCGTCCAGT
    GGTGAAAGCT GGGAGACTCT GCCAGGGAAA GAGGAGCACG ACTCTGAGCA GGTCAGAGTG
    AATGCTGGCG CCGGCGCCAG TGCCAGCCCG GGTACCGGCG CCAGCGCCGG CAGCAACAGC
    AGCAGTGAAC TGGAAGACCT CCGAGGACCA CCTCTTCAGG AAGAGGAACA CGCATCCCCC
    AGAGAACGAG AAGCTCCTTG GCTCCAGTAC AACGAAAATG AGAGTAGCAG TGAGGGGGAT
    AATGAATCTG CTCAGGAGTA TCTACAGCCT GGGGTGTTCA TGCTCGATGG AAACAACAAT
    CTTGAAGATG ACTCCAGCGT GAGTGAAGAC CTAGAAGTGG ATTGGAGCCT CTTTGATGGG
    TTCGCAGATG GCTTGGGGGT GGCCGAAGCC ATTTCCTACG TGGATCCTCA GTTTCTCACC
    TACATGGCAC TTGAAGAACG CCTGGCCCAG GCAATGGAAA CTGCCCTGGC GCACCTAGAG
    TCTCTCGCAG TGGACGTGGA GGTGGCCAAC CCACCGGCGA GCAAGGAGAG CATCGACACT
    CTCCCTGAGA TCCTGGTCAC TGAAGATCAC AGTGCAGTGG GGCAGGAAAT GTGTTGCCCC
    ATCTGTTGTA GTGAATATGT GAAGGGGGAG GTGGCAACTG AGCTGCCTTG CCACCACTAT
    TTCCACAAGC CGTGTGTGTC CATCTGGCTT CAGAAATCAG GCACCTGCCC TGTGTGCCGC
    TGCATGTTCC CTCCCCCGCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967348.1
    CDS373..1716
    Translation

    Target ORF information:

    RefSeq Version XM_014111873.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris praja ring finger ubiquitin ligase 1 (PJA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGTACTTCC
    AGGTGGAGGG ATACAGCCAA CGCTGAAGGC CACTCGGATG GCCTAGCAAG AAGAGCCAGA
    GGTGAGAGTT CAGGTGGCTA CTCTGAGCTG AAGTACCCCG AAGACAAGAG GGAAGCCAGG
    AATGACCAAG TGAAGCCAGA AAAGGTACCA AGACGGCGGC GAACCATGGC TGACCCTGAC
    TTCTGGACGT ACAGCGACGA TTACTACAAA TACTGTGATG AAGACTCTGA CAGTGACAAA
    GAGTGGACTG CTGCTCTGCG GCGGAAGTAT CGAGGTCGAG AGCAGAATCT GTCGTCCAGT
    GGTGAAAGCT GGGAGACTCT GCCAGGGAAA GAGGAGCACG ACTCTGAGCA GGTCAGAGTG
    AATGCTGGCG CCGGCGCCAG TGCCAGCCCG GGTACCGGCG CCAGCGCCGG CAGCAACAGC
    AGCAGTGAAC TGGAAGACCT CCGAGGACCA CCTCTTCAGG AAGAGGAACA CGCATCCCCC
    AGAGAACGAG AAGCTCCTTG GCTCCAGTAC AACGAAAATG AGAGTAGCAG TGAGGGGGAT
    AATGAATCTG CTCAGGAGTA TCTACAGCCT GGGGTGTTCA TGCTCGATGG AAACAACAAT
    CTTGAAGATG ACTCCAGCGT GAGTGAAGAC CTAGAAGTGG ATTGGAGCCT CTTTGATGGG
    TTCGCAGATG GCTTGGGGGT GGCCGAAGCC ATTTCCTACG TGGATCCTCA GTTTCTCACC
    TACATGGCAC TTGAAGAACG CCTGGCCCAG GCAATGGAAA CTGCCCTGGC GCACCTAGAG
    TCTCTCGCAG TGGACGTGGA GGTGGCCAAC CCACCGGCGA GCAAGGAGAG CATCGACACT
    CTCCCTGAGA TCCTGGTCAC TGAAGATCAC AGTGCAGTGG GGCAGGAAAT GTGTTGCCCC
    ATCTGTTGTA GTGAATATGT GAAGGGGGAG GTGGCAACTG AGCTGCCTTG CCACCACTAT
    TTCCACAAGC CGTGTGTGTC CATCTGGCTT CAGAAATCAG GCACCTGCCC TGTGTGCCGC
    TGCATGTTCC CTCCCCCGCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32481
    Clone ID Related Accession (Same CDS sequence) XM_014111873.2 , XM_014111873.1
    Accession Version XM_014111873.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGTACTTCC
    AGGTGGAGGG ATACAGCCAA CGCTGAAGGC CACTCGGATG GCCTAGCAAG AAGAGCCAGA
    GGTGAGAGTT CAGGTGGCTA CTCTGAGCTG AAGTACCCCG AAGACAAGAG GGAAGCCAGG
    AATGACCAAG TGAAGCCAGA AAAGGTACCA AGACGGCGGC GAACCATGGC TGACCCTGAC
    TTCTGGACGT ACAGCGACGA TTACTACAAA TACTGTGATG AAGACTCTGA CAGTGACAAA
    GAGTGGACTG CTGCTCTGCG GCGGAAGTAT CGAGGTCGAG AGCAGAATCT GTCGTCCAGT
    GGTGAAAGCT GGGAGACTCT GCCAGGGAAA GAGGAGCACG ACTCTGAGCA GGTCAGAGTG
    AATGCTGGCG CCGGCGCCAG TGCCAGCCCG GGTACCGGCG CCAGCGCCGG CAGCAACAGC
    AGCAGTGAAC TGGAAGACCT CCGAGGACCA CCTCTTCAGG AAGAGGAACA CGCATCCCCC
    AGAGAACGAG AAGCTCCTTG GCTCCAGTAC AACGAAAATG AGAGTAGCAG TGAGGGGGAT
    AATGAATCTG CTCAGGAGTA TCTACAGCCT GGGGTGTTCA TGCTCGATGG AAACAACAAT
    CTTGAAGATG ACTCCAGCGT GAGTGAAGAC CTAGAAGTGG ATTGGAGCCT CTTTGATGGG
    TTCGCAGATG GCTTGGGGGT GGCCGAAGCC ATTTCCTACG TGGATCCTCA GTTTCTCACC
    TACATGGCAC TTGAAGAACG CCTGGCCCAG GCAATGGAAA CTGCCCTGGC GCACCTAGAG
    TCTCTCGCAG TGGACGTGGA GGTGGCCAAC CCACCGGCGA GCAAGGAGAG CATCGACACT
    CTCCCTGAGA TCCTGGTCAC TGAAGATCAC AGTGCAGTGG GGCAGGAAAT GTGTTGCCCC
    ATCTGTTGTA GTGAATATGT GAAGGGGGAG GTGGCAACTG AGCTGCCTTG CCACCACTAT
    TTCCACAAGC CGTGTGTGTC CATCTGGCTT CAGAAATCAG GCACCTGCCC TGTGTGCCGC
    TGCATGTTCC CTCCCCCGCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967348.1
    CDS379..1722
    Translation

    Target ORF information:

    RefSeq Version XM_014111873.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris praja ring finger 1, E3 ubiquitin protein ligase (PJA1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGGTCAGG AATCTAGCAA GCCCGTATGG CCCAAGCCAG CAGGAGGGTA TCAGTCCAAT 
    ACAGGCAGGA GGTATGGAAG AAGGCATGCT TATGTCAGTT TCAGGCCATC CTCGAGCCAG
    CAGGACAGGA TTTCCAGCCA GAGAAAGACA ACATCCGAAG TCCCAATGCA CAGATCAGCC
    CCCAGTCAAA CCACCAAGAG GAGCCGGTCA CCATTTTCCA TCACCCGTCG TAGTTGGGAG
    GACAGTGAGA GCTCTGGAGC CAGCCTGAAT GTTGATAATG AGGACTACTC CAGTACTTCC
    AGGTGGAGGG ATACAGCCAA CGCTGAAGGC CACTCGGATG GCCTAGCAAG AAGAGCCAGA
    GGTGAGAGTT CAGGTGGCTA CTCTGAGCTG AAGTACCCCG AAGACAAGAG GGAAGCCAGG
    AATGACCAAG TGAAGCCAGA AAAGGTACCA AGACGGCGGC GAACCATGGC TGACCCTGAC
    TTCTGGACGT ACAGCGACGA TTACTACAAA TACTGTGATG AAGACTCTGA CAGTGACAAA
    GAGTGGACTG CTGCTCTGCG GCGGAAGTAT CGAGGTCGAG AGCAGAATCT GTCGTCCAGT
    GGTGAAAGCT GGGAGACTCT GCCAGGGAAA GAGGAGCACG ACTCTGAGCA GGTCAGAGTG
    AATGCTGGCG CCGGCGCCAG TGCCAGCCCG GGTACCGGCG CCAGCGCCGG CAGCAACAGC
    AGCAGTGAAC TGGAAGACCT CCGAGGACCA CCTCTTCAGG AAGAGGAACA CGCATCCCCC
    AGAGAACGAG AAGCTCCTTG GCTCCAGTAC AACGAAAATG AGAGTAGCAG TGAGGGGGAT
    AATGAATCTG CTCAGGAGTA TCTACAGCCT GGGGTGTTCA TGCTCGATGG AAACAACAAT
    CTTGAAGATG ACTCCAGCGT GAGTGAAGAC CTAGAAGTGG ATTGGAGCCT CTTTGATGGG
    TTCGCAGATG GCTTGGGGGT GGCCGAAGCC ATTTCCTACG TGGATCCTCA GTTTCTCACC
    TACATGGCAC TTGAAGAACG CCTGGCCCAG GCAATGGAAA CTGCCCTGGC GCACCTAGAG
    TCTCTCGCAG TGGACGTGGA GGTGGCCAAC CCACCGGCGA GCAAGGAGAG CATCGACACT
    CTCCCTGAGA TCCTGGTCAC TGAAGATCAC AGTGCAGTGG GGCAGGAAAT GTGTTGCCCC
    ATCTGTTGTA GTGAATATGT GAAGGGGGAG GTGGCAACTG AGCTGCCTTG CCACCACTAT
    TTCCACAAGC CGTGTGTGTC CATCTGGCTT CAGAAATCAG GCACCTGCCC TGTGTGCCGC
    TGCATGTTCC CTCCCCCGCT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.