Pja1 cDNA ORF clone, Mus musculus(house mouse)

The following Pja1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pja1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu23409 NM_001290556.1
Latest version!
Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu23415 NM_008853.3
Latest version!
Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $188.30
$269.00
OMu23416 NM_001290555.1
Latest version!
Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu23416 XM_011247544.1
Latest version!
Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu68438 XM_011247546.1
Latest version!
Mus musculus praja ring finger 1, E3 ubiquitin protein ligase (Pja1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu68438 XM_030251256.1
Latest version!
Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu23408 NM_001083110.2
Latest version!
Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OMu23408 XM_030251255.1
Latest version!
Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu23408 XM_011247545.1
Latest version!
Mus musculus praja ring finger 1, E3 ubiquitin protein ligase (Pja1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu23409
    Clone ID Related Accession (Same CDS sequence) NM_001290556.1
    Accession Version NM_001290556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Praja-1 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL831752.7. On or before Jul 31, 2015 this sequence version replaced XM_011247547.1, XM_006527899.1. Transcript Variant: This variant (4) lacks an alternate in-frame segment, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.234832.1, SRR1660811.108222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGT
    ACTTCCAGGT GGCGGGATGC TGCCGATGCT GAGGAGGCTC ATGCCGAGGG CCTAGCCAGA
    AGAGGCCGAG GTGAGGCTGC CAGCAGCTCA GAGCCAAGGT ATGCTGAAGA CCAGGATGCC
    AGGAGTGAAC AAGCGAAGGC AGACAAAGTG CCAAGACGGC GGCGAACCAT GGCAGACCCT
    GACTTCTGGG CATACACCGA CGATTACTAC CGATACTACG AGGAAGATTC TGACAGCGAC
    AAAGAGTGGA TGGCTGCCCT GCGCAGGAAG TACCGAAGCC GAGAGCAACC CCAGTCCTCC
    AGCGGAGAAA GCTGGGAGCT TCTGCCAGGA AAGGAAGAAC TGGAACGTCA GCAAGCCGGA
    GCTGGGAGCC TCGCCAGTGC TGGCAGCAAT GGCAGTGGTT ATCCTGAAGA AGTACAAGAC
    CCATCTCTTC AGGAGGAAGA ACAGGCCTCT CTGGAAGAAG GAGAAATCCC TTGGCTTCGC
    TACAATGAGA ATGAAAGCAG CAGCGAGGGT GATAATGAGT CTACCCATGA GCTCATACAG
    CCTGGGATGT TCATGCTGGA TGGAAACAAC AACCTGGAAG ATGACTCCAG CGTGAGCGAA
    GACCTCGAAG TGGACTGGAG CCTGTTTGAT GGGTTTGCCG ATGGCTTGGG AGTGGCCGAA
    GCCATCTCCT ACGTGGATCC TCAGTTCCTC ACCTACATGG CTCTGGAAGA GCGTCTGGCC
    CAGGCAATGG AGACGGCCCT GGCACACTTG GAGTCTCTCG CCGTTGATGT CGAAGTGGCC
    AACCCACCAG CAAGCAAGGA GAGCATTGAT GCCCTTCCTG AGATCCTGGT CACCGAAGAT
    CATGGTGCAG TGGGCCAGGA AATGTGCTGT CCTATCTGCT GCAGCGAATA TGTGAAGGGG
    GAGGTGGCAA CTGAGCTACC ATGCCACCAC TATTTCCACA AGCCCTGCGT GTCCATCTGG
    CTTCAGAAGT CTGGCACCTG CCCAGTGTGC CGCTGCATGT TCCCTCCCCC GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277485.1
    CDS133..1329
    Translation

    Target ORF information:

    RefSeq Version NM_001290556.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGT
    ACTTCCAGGT GGCGGGATGC TGCCGATGCT GAGGAGGCTC ATGCCGAGGG CCTAGCCAGA
    AGAGGCCGAG GTGAGGCTGC CAGCAGCTCA GAGCCAAGGT ATGCTGAAGA CCAGGATGCC
    AGGAGTGAAC AAGCGAAGGC AGACAAAGTG CCAAGACGGC GGCGAACCAT GGCAGACCCT
    GACTTCTGGG CATACACCGA CGATTACTAC CGATACTACG AGGAAGATTC TGACAGCGAC
    AAAGAGTGGA TGGCTGCCCT GCGCAGGAAG TACCGAAGCC GAGAGCAACC CCAGTCCTCC
    AGCGGAGAAA GCTGGGAGCT TCTGCCAGGA AAGGAAGAAC TGGAACGTCA GCAAGCCGGA
    GCTGGGAGCC TCGCCAGTGC TGGCAGCAAT GGCAGTGGTT ATCCTGAAGA AGTACAAGAC
    CCATCTCTTC AGGAGGAAGA ACAGGCCTCT CTGGAAGAAG GAGAAATCCC TTGGCTTCGC
    TACAATGAGA ATGAAAGCAG CAGCGAGGGT GATAATGAGT CTACCCATGA GCTCATACAG
    CCTGGGATGT TCATGCTGGA TGGAAACAAC AACCTGGAAG ATGACTCCAG CGTGAGCGAA
    GACCTCGAAG TGGACTGGAG CCTGTTTGAT GGGTTTGCCG ATGGCTTGGG AGTGGCCGAA
    GCCATCTCCT ACGTGGATCC TCAGTTCCTC ACCTACATGG CTCTGGAAGA GCGTCTGGCC
    CAGGCAATGG AGACGGCCCT GGCACACTTG GAGTCTCTCG CCGTTGATGT CGAAGTGGCC
    AACCCACCAG CAAGCAAGGA GAGCATTGAT GCCCTTCCTG AGATCCTGGT CACCGAAGAT
    CATGGTGCAG TGGGCCAGGA AATGTGCTGT CCTATCTGCT GCAGCGAATA TGTGAAGGGG
    GAGGTGGCAA CTGAGCTACC ATGCCACCAC TATTTCCACA AGCCCTGCGT GTCCATCTGG
    CTTCAGAAGT CTGGCACCTG CCCAGTGTGC CGCTGCATGT TCCCTCCCCC GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23415
    Clone ID Related Accession (Same CDS sequence) NM_008853.3
    Accession Version NM_008853.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-22
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Praja-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL831752.7. On Mar 16, 2007 this sequence version replaced NM_008853.2. Transcript Variant: This variant (2) lacks an alternate in-frame segment, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032879.2
    CDS133..1320
    Translation

    Target ORF information:

    RefSeq Version NM_008853.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_008853.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23416
    Clone ID Related Accession (Same CDS sequence) XM_011247544.1 , NM_001290555.1
    Accession Version NM_001290555.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Praja-1 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN695027.1, AK145943.1 and CD741074.1. On Mar 14, 2014 this sequence version replaced XM_006527897.1. Transcript Variant: This variant (3) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a longer N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.91951.1, AK145943.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGTCAGG GACCTAGCAA GCCTGTTCGG CCCAGAGCAG CAGGAGGGTA TCAGTCAGGT 
    ACAGGCAGGA GTTATGGAAG GAGGCATGCT TCTGTCAGTT TCAGGCCATC CATGAGCCAC
    CAGGAAAGGA TTGCCAGCCA GAGGAGGACA ACAGCCGAAG TCCCAATGCA CAGATCAACT
    GCCAATCAAA GCAAGAGGAG CCGGTCACCA TTTGCCAGCA CACGTCGTCG CTGGGATGAC
    AGCGAGAGCT CGGGAGCCAG CCTGGCTGTT GAGAGTGAGG ATTATTCCAG GTATCCTCCA
    AGAGAGTACA GGGCCTCGGG GAGCCGAAGA GGATTGGCTT ATGGACACAT TGACACTGTA
    GTGGCTCGTG ATAGTGAGGA GGAGGGGGCT GGGCCTGTTG ACCGACTGCC AGTGAGAGGG
    AAAGCTGGCA AGTTTAAGGA TGATCCCGAG AAGGGGGCAA GGTCTTCCCG CTTTACTAGT
    GTTAACCATG ATGCGAAGGA AGAGTGTGGC AAGGTAGAAT CACCCCCTGC AGCGAGGTGC
    TCGGCTCGCA GAGCTGAGCT CTCGAAGCAG AATGGCTCCT CGGCCTCTCA GATTTCTTCT
    GCTGAAGGCA GGGCAGCTGC AAAAGGTAAC AACAGCTTGG AGAGGGAGAG GCAGAATTTA
    CCAGCTCGTC CTAGCAGGGC TCCTGTGAGT ATTTGTGGTG GTGGGGAAAA CACCCCAAAG
    AGTGCCGAGG AACCGGTGGT GAGACCCAAA GTCCGGAATG TGGCGACTCC AAACTGCATG
    AAACCCAAAG TGTTTTTTGA TACTGATGAT GATGATGATG TACCACACAG TACTTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001277484.1
    CDS308..2155
    Translation

    Target ORF information:

    RefSeq Version NM_001290555.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGTCAGG GACCTAGCAA GCCTGTTCGG CCCAGAGCAG CAGGAGGGTA TCAGTCAGGT 
    ACAGGCAGGA GTTATGGAAG GAGGCATGCT TCTGTCAGTT TCAGGCCATC CATGAGCCAC
    CAGGAAAGGA TTGCCAGCCA GAGGAGGACA ACAGCCGAAG TCCCAATGCA CAGATCAACT
    GCCAATCAAA GCAAGAGGAG CCGGTCACCA TTTGCCAGCA CACGTCGTCG CTGGGATGAC
    AGCGAGAGCT CGGGAGCCAG CCTGGCTGTT GAGAGTGAGG ATTATTCCAG GTATCCTCCA
    AGAGAGTACA GGGCCTCGGG GAGCCGAAGA GGATTGGCTT ATGGACACAT TGACACTGTA
    GTGGCTCGTG ATAGTGAGGA GGAGGGGGCT GGGCCTGTTG ACCGACTGCC AGTGAGAGGG
    AAAGCTGGCA AGTTTAAGGA TGATCCCGAG AAGGGGGCAA GGTCTTCCCG CTTTACTAGT
    GTTAACCATG ATGCGAAGGA AGAGTGTGGC AAGGTAGAAT CACCCCCTGC AGCGAGGTGC
    TCGGCTCGCA GAGCTGAGCT CTCGAAGCAG AATGGCTCCT CGGCCTCTCA GATTTCTTCT
    GCTGAAGGCA GGGCAGCTGC AAAAGGTAAC AACAGCTTGG AGAGGGAGAG GCAGAATTTA
    CCAGCTCGTC CTAGCAGGGC TCCTGTGAGT ATTTGTGGTG GTGGGGAAAA CACCCCAAAG
    AGTGCCGAGG AACCGGTGGT GAGACCCAAA GTCCGGAATG TGGCGACTCC AAACTGCATG
    AAACCCAAAG TGTTTTTTGA TACTGATGAT GATGATGATG TACCACACAG TACTTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23416
    Clone ID Related Accession (Same CDS sequence) XM_011247544.1 , NM_001290555.1
    Accession Version XM_011247544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGTCAGG GACCTAGCAA GCCTGTTCGG CCCAGAGCAG CAGGAGGGTA TCAGTCAGGT 
    ACAGGCAGGA GTTATGGAAG GAGGCATGCT TCTGTCAGTT TCAGGCCATC CATGAGCCAC
    CAGGAAAGGA TTGCCAGCCA GAGGAGGACA ACAGCCGAAG TCCCAATGCA CAGATCAACT
    GCCAATCAAA GCAAGAGGAG CCGGTCACCA TTTGCCAGCA CACGTCGTCG CTGGGATGAC
    AGCGAGAGCT CGGGAGCCAG CCTGGCTGTT GAGAGTGAGG ATTATTCCAG GTATCCTCCA
    AGAGAGTACA GGGCCTCGGG GAGCCGAAGA GGATTGGCTT ATGGACACAT TGACACTGTA
    GTGGCTCGTG ATAGTGAGGA GGAGGGGGCT GGGCCTGTTG ACCGACTGCC AGTGAGAGGG
    AAAGCTGGCA AGTTTAAGGA TGATCCCGAG AAGGGGGCAA GGTCTTCCCG CTTTACTAGT
    GTTAACCATG ATGCGAAGGA AGAGTGTGGC AAGGTAGAAT CACCCCCTGC AGCGAGGTGC
    TCGGCTCGCA GAGCTGAGCT CTCGAAGCAG AATGGCTCCT CGGCCTCTCA GATTTCTTCT
    GCTGAAGGCA GGGCAGCTGC AAAAGGTAAC AACAGCTTGG AGAGGGAGAG GCAGAATTTA
    CCAGCTCGTC CTAGCAGGGC TCCTGTGAGT ATTTGTGGTG GTGGGGAAAA CACCCCAAAG
    AGTGCCGAGG AACCGGTGGT GAGACCCAAA GTCCGGAATG TGGCGACTCC AAACTGCATG
    AAACCCAAAG TGTTTTTTGA TACTGATGAT GATGATGATG TACCACACAG TACTTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011245846.1
    CDS641..2488
    Translation

    Target ORF information:

    RefSeq Version XM_011247544.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGTCAGG GACCTAGCAA GCCTGTTCGG CCCAGAGCAG CAGGAGGGTA TCAGTCAGGT 
    ACAGGCAGGA GTTATGGAAG GAGGCATGCT TCTGTCAGTT TCAGGCCATC CATGAGCCAC
    CAGGAAAGGA TTGCCAGCCA GAGGAGGACA ACAGCCGAAG TCCCAATGCA CAGATCAACT
    GCCAATCAAA GCAAGAGGAG CCGGTCACCA TTTGCCAGCA CACGTCGTCG CTGGGATGAC
    AGCGAGAGCT CGGGAGCCAG CCTGGCTGTT GAGAGTGAGG ATTATTCCAG GTATCCTCCA
    AGAGAGTACA GGGCCTCGGG GAGCCGAAGA GGATTGGCTT ATGGACACAT TGACACTGTA
    GTGGCTCGTG ATAGTGAGGA GGAGGGGGCT GGGCCTGTTG ACCGACTGCC AGTGAGAGGG
    AAAGCTGGCA AGTTTAAGGA TGATCCCGAG AAGGGGGCAA GGTCTTCCCG CTTTACTAGT
    GTTAACCATG ATGCGAAGGA AGAGTGTGGC AAGGTAGAAT CACCCCCTGC AGCGAGGTGC
    TCGGCTCGCA GAGCTGAGCT CTCGAAGCAG AATGGCTCCT CGGCCTCTCA GATTTCTTCT
    GCTGAAGGCA GGGCAGCTGC AAAAGGTAAC AACAGCTTGG AGAGGGAGAG GCAGAATTTA
    CCAGCTCGTC CTAGCAGGGC TCCTGTGAGT ATTTGTGGTG GTGGGGAAAA CACCCCAAAG
    AGTGCCGAGG AACCGGTGGT GAGACCCAAA GTCCGGAATG TGGCGACTCC AAACTGCATG
    AAACCCAAAG TGTTTTTTGA TACTGATGAT GATGATGATG TACCACACAG TACTTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68438
    Clone ID Related Accession (Same CDS sequence) XM_011247546.1 , XM_030251256.1
    Accession Version XM_011247546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGTCAGG GACCTAGCAA GCCTGTTCGG CCCAGAGCAG CAGGAGGGTA TCAGTCAGGT 
    ACAGGCAGGA GTTATGGAAG GAGGCATGCT TCTGTCAGTT TCAGGCCATC CATGAGCCAC
    CAGGAAAGGA TTGCCAGCCA GAGGAGGACA ACAGCCGAAG TCCCAATGCA CAGATCAACT
    GCCAATCAAA GCAAGAGGAG CCGGTCACCA TTTGCCAGCA CACGTCGTCG CTGGGATGAC
    AGCGAGAGCT CGGGAGCCAG CCTGGCTGTT GAGAGTGAGG ATTATTCCAG TACTTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011245848.1
    CDS641..1948
    Translation

    Target ORF information:

    RefSeq Version XM_011247546.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus praja ring finger 1, E3 ubiquitin protein ligase (Pja1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGTCAGG GACCTAGCAA GCCTGTTCGG CCCAGAGCAG CAGGAGGGTA TCAGTCAGGT 
    ACAGGCAGGA GTTATGGAAG GAGGCATGCT TCTGTCAGTT TCAGGCCATC CATGAGCCAC
    CAGGAAAGGA TTGCCAGCCA GAGGAGGACA ACAGCCGAAG TCCCAATGCA CAGATCAACT
    GCCAATCAAA GCAAGAGGAG CCGGTCACCA TTTGCCAGCA CACGTCGTCG CTGGGATGAC
    AGCGAGAGCT CGGGAGCCAG CCTGGCTGTT GAGAGTGAGG ATTATTCCAG TACTTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68438
    Clone ID Related Accession (Same CDS sequence) XM_011247546.1 , XM_030251256.1
    Accession Version XM_030251256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGTCAGG GACCTAGCAA GCCTGTTCGG CCCAGAGCAG CAGGAGGGTA TCAGTCAGGT 
    ACAGGCAGGA GTTATGGAAG GAGGCATGCT TCTGTCAGTT TCAGGCCATC CATGAGCCAC
    CAGGAAAGGA TTGCCAGCCA GAGGAGGACA ACAGCCGAAG TCCCAATGCA CAGATCAACT
    GCCAATCAAA GCAAGAGGAG CCGGTCACCA TTTGCCAGCA CACGTCGTCG CTGGGATGAC
    AGCGAGAGCT CGGGAGCCAG CCTGGCTGTT GAGAGTGAGG ATTATTCCAG TACTTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107116.1
    CDS474..1781
    Translation

    Target ORF information:

    RefSeq Version XM_030251256.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGTCAGG GACCTAGCAA GCCTGTTCGG CCCAGAGCAG CAGGAGGGTA TCAGTCAGGT 
    ACAGGCAGGA GTTATGGAAG GAGGCATGCT TCTGTCAGTT TCAGGCCATC CATGAGCCAC
    CAGGAAAGGA TTGCCAGCCA GAGGAGGACA ACAGCCGAAG TCCCAATGCA CAGATCAACT
    GCCAATCAAA GCAAGAGGAG CCGGTCACCA TTTGCCAGCA CACGTCGTCG CTGGGATGAC
    AGCGAGAGCT CGGGAGCCAG CCTGGCTGTT GAGAGTGAGG ATTATTCCAG TACTTCCAGG
    TGGCGGGATG CTGCCGATGC TGAGGAGGCT CATGCCGAGG GCCTAGCCAG AAGAGGCCGA
    GGTGAGGCTG CCAGCAGCTC AGAGCCAAGG TATGCTGAAG ACCAGGATGC CAGGAGTGAA
    CAAGCGAAGG CAGACAAAGT GCCAAGACGG CGGCGAACCA TGGCAGACCC TGACTTCTGG
    GCATACACCG ACGATTACTA CCGATACTAC GAGGAAGATT CTGACAGCGA CAAAGAGTGG
    ATGGCTGCCC TGCGCAGGAA GTACCGAAGC CGAGAGCAAC CCCAGTCCTC CAGCGGAGAA
    AGCTGGGAGC TTCTGCCAGG AAAGGAAGAA CTGGAACGTC AGCAAGCCGG AGCTGGGAGC
    CTCGCCAGTG CTGGCAGCAA TGGCAGTGGT TATCCTGAAG AAGTACAAGA CCCATCTCTT
    CAGGAGGAAG AACAGGCCTC TCTGGAAGAA GGAGAAATCC CTTGGCTTCG CTACAATGAG
    AATGAAAGCA GCAGCGAGGG TGATAATGAG TCTACCCATG AGCTCATACA GCCTGGGATG
    TTCATGCTGG ATGGAAACAA CAACCTGGAA GATGACTCCA GCGTGAGCGA AGACCTCGAA
    GTGGACTGGA GCCTGTTTGA TGGGTTTGCC GATGGCTTGG GAGTGGCCGA AGCCATCTCC
    TACGTGGATC CTCAGTTCCT CACCTACATG GCTCTGGAAG AGCGTCTGGC CCAGGCAATG
    GAGACGGCCC TGGCACACTT GGAGTCTCTC GCCGTTGATG TCGAAGTGGC CAACCCACCA
    GCAAGCAAGG AGAGCATTGA TGCCCTTCCT GAGATCCTGG TCACCGAAGA TCATGGTGCA
    GTGGGCCAGG AAATGTGCTG TCCTATCTGC TGCAGCGAAT ATGTGAAGGG GGAGGTGGCA
    ACTGAGCTAC CATGCCACCA CTATTTCCAC AAGCCCTGCG TGTCCATCTG GCTTCAGAAG
    TCTGGCACCT GCCCAGTGTG CCGCTGCATG TTCCCTCCCC CGCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23408
    Clone ID Related Accession (Same CDS sequence) XM_011247545.1 , XM_030251255.1 , NM_001083110.2
    Accession Version NM_001083110.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Praja-1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN695027.1, AK135382.1, AK145943.1 and CD741074.1. On Mar 14, 2014 this sequence version replaced NM_001083110.1. Transcript Variant: This variant (1) encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: CN695027.1, SRR1660813.138391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGG
    TATCCTCCAA GAGAGTACAG GGCCTCGGGG AGCCGAAGAG GATTGGCTTA TGGACACATT
    GACACTGTAG TGGCTCGTGA TAGTGAGGAG GAGGGGGCTG GGCCTGTTGA CCGACTGCCA
    GTGAGAGGGA AAGCTGGCAA GTTTAAGGAT GATCCCGAGA AGGGGGCAAG GTCTTCCCGC
    TTTACTAGTG TTAACCATGA TGCGAAGGAA GAGTGTGGCA AGGTAGAATC ACCCCCTGCA
    GCGAGGTGCT CGGCTCGCAG AGCTGAGCTC TCGAAGCAGA ATGGCTCCTC GGCCTCTCAG
    ATTTCTTCTG CTGAAGGCAG GGCAGCTGCA AAAGGTAACA ACAGCTTGGA GAGGGAGAGG
    CAGAATTTAC CAGCTCGTCC TAGCAGGGCT CCTGTGAGTA TTTGTGGTGG TGGGGAAAAC
    ACCCCAAAGA GTGCCGAGGA ACCGGTGGTG AGACCCAAAG TCCGGAATGT GGCGACTCCA
    AACTGCATGA AACCCAAAGT GTTTTTTGAT ACTGATGATG ATGATGATGT ACCACACAGT
    ACTTCCAGGT GGCGGGATGC TGCCGATGCT GAGGAGGCTC ATGCCGAGGG CCTAGCCAGA
    AGAGGCCGAG GTGAGGCTGC CAGCAGCTCA GAGCCAAGGT ATGCTGAAGA CCAGGATGCC
    AGGAGTGAAC AAGCGAAGGC AGACAAAGTG CCAAGACGGC GGCGAACCAT GGCAGACCCT
    GACTTCTGGG CATACACCGA CGATTACTAC CGATACTACG AGGAAGATTC TGACAGCGAC
    AAAGAGTGGA TGGCTGCCCT GCGCAGGAAG TACCGAAGCC GAGAGCAACC CCAGTCCTCC
    AGCGGAGAAA GCTGGGAGCT TCTGCCAGGA AAGGAAGAAC TGGAACGTCA GCAAGCCGGA
    GCTGGGAGCC TCGCCAGTGC TGGCAGCAAT GGCAGTGGTT ATCCTGAAGA AGTACAAGAC
    CCATCTCTTC AGGAGGAAGA ACAGGCCTCT CTGGAAGAAG GAGAAATCCC TTGGCTTCGC
    TACAATGAGA ATGAAAGCAG CAGCGAGGGT GATAATGAGT CTACCCATGA GCTCATACAG
    CCTGGGATGT TCATGCTGGA TGGAAACAAC AACCTGGAAG ATGACTCCAG CGTGAGCGAA
    GACCTCGAAG TGGACTGGAG CCTGTTTGAT GGGTTTGCCG ATGGCTTGGG AGTGGCCGAA
    GCCATCTCCT ACGTGGATCC TCAGTTCCTC ACCTACATGG CTCTGGAAGA GCGTCTGGCC
    CAGGCAATGG AGACGGCCCT GGCACACTTG GAGTCTCTCG CCGTTGATGT CGAAGTGGCC
    AACCCACCAG CAAGCAAGGA GAGCATTGAT GCCCTTCCTG AGATCCTGGT CACCGAAGAT
    CATGGTGCAG TGGGCCAGGA AATGTGCTGT CCTATCTGCT GCAGCGAATA TGTGAAGGGG
    GAGGTGGCAA CTGAGCTACC ATGCCACCAC TATTTCCACA AGCCCTGCGT GTCCATCTGG
    CTTCAGAAGT CTGGCACCTG CCCAGTGTGC CGCTGCATGT TCCCTCCCCC GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001076579.1
    CDS133..1869
    Translation

    Target ORF information:

    RefSeq Version NM_001083110.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083110.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGG
    TATCCTCCAA GAGAGTACAG GGCCTCGGGG AGCCGAAGAG GATTGGCTTA TGGACACATT
    GACACTGTAG TGGCTCGTGA TAGTGAGGAG GAGGGGGCTG GGCCTGTTGA CCGACTGCCA
    GTGAGAGGGA AAGCTGGCAA GTTTAAGGAT GATCCCGAGA AGGGGGCAAG GTCTTCCCGC
    TTTACTAGTG TTAACCATGA TGCGAAGGAA GAGTGTGGCA AGGTAGAATC ACCCCCTGCA
    GCGAGGTGCT CGGCTCGCAG AGCTGAGCTC TCGAAGCAGA ATGGCTCCTC GGCCTCTCAG
    ATTTCTTCTG CTGAAGGCAG GGCAGCTGCA AAAGGTAACA ACAGCTTGGA GAGGGAGAGG
    CAGAATTTAC CAGCTCGTCC TAGCAGGGCT CCTGTGAGTA TTTGTGGTGG TGGGGAAAAC
    ACCCCAAAGA GTGCCGAGGA ACCGGTGGTG AGACCCAAAG TCCGGAATGT GGCGACTCCA
    AACTGCATGA AACCCAAAGT GTTTTTTGAT ACTGATGATG ATGATGATGT ACCACACAGT
    ACTTCCAGGT GGCGGGATGC TGCCGATGCT GAGGAGGCTC ATGCCGAGGG CCTAGCCAGA
    AGAGGCCGAG GTGAGGCTGC CAGCAGCTCA GAGCCAAGGT ATGCTGAAGA CCAGGATGCC
    AGGAGTGAAC AAGCGAAGGC AGACAAAGTG CCAAGACGGC GGCGAACCAT GGCAGACCCT
    GACTTCTGGG CATACACCGA CGATTACTAC CGATACTACG AGGAAGATTC TGACAGCGAC
    AAAGAGTGGA TGGCTGCCCT GCGCAGGAAG TACCGAAGCC GAGAGCAACC CCAGTCCTCC
    AGCGGAGAAA GCTGGGAGCT TCTGCCAGGA AAGGAAGAAC TGGAACGTCA GCAAGCCGGA
    GCTGGGAGCC TCGCCAGTGC TGGCAGCAAT GGCAGTGGTT ATCCTGAAGA AGTACAAGAC
    CCATCTCTTC AGGAGGAAGA ACAGGCCTCT CTGGAAGAAG GAGAAATCCC TTGGCTTCGC
    TACAATGAGA ATGAAAGCAG CAGCGAGGGT GATAATGAGT CTACCCATGA GCTCATACAG
    CCTGGGATGT TCATGCTGGA TGGAAACAAC AACCTGGAAG ATGACTCCAG CGTGAGCGAA
    GACCTCGAAG TGGACTGGAG CCTGTTTGAT GGGTTTGCCG ATGGCTTGGG AGTGGCCGAA
    GCCATCTCCT ACGTGGATCC TCAGTTCCTC ACCTACATGG CTCTGGAAGA GCGTCTGGCC
    CAGGCAATGG AGACGGCCCT GGCACACTTG GAGTCTCTCG CCGTTGATGT CGAAGTGGCC
    AACCCACCAG CAAGCAAGGA GAGCATTGAT GCCCTTCCTG AGATCCTGGT CACCGAAGAT
    CATGGTGCAG TGGGCCAGGA AATGTGCTGT CCTATCTGCT GCAGCGAATA TGTGAAGGGG
    GAGGTGGCAA CTGAGCTACC ATGCCACCAC TATTTCCACA AGCCCTGCGT GTCCATCTGG
    CTTCAGAAGT CTGGCACCTG CCCAGTGTGC CGCTGCATGT TCCCTCCCCC GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23408
    Clone ID Related Accession (Same CDS sequence) XM_011247545.1 , XM_030251255.1 , NM_001083110.2
    Accession Version XM_011247545.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X2
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGG
    TATCCTCCAA GAGAGTACAG GGCCTCGGGG AGCCGAAGAG GATTGGCTTA TGGACACATT
    GACACTGTAG TGGCTCGTGA TAGTGAGGAG GAGGGGGCTG GGCCTGTTGA CCGACTGCCA
    GTGAGAGGGA AAGCTGGCAA GTTTAAGGAT GATCCCGAGA AGGGGGCAAG GTCTTCCCGC
    TTTACTAGTG TTAACCATGA TGCGAAGGAA GAGTGTGGCA AGGTAGAATC ACCCCCTGCA
    GCGAGGTGCT CGGCTCGCAG AGCTGAGCTC TCGAAGCAGA ATGGCTCCTC GGCCTCTCAG
    ATTTCTTCTG CTGAAGGCAG GGCAGCTGCA AAAGGTAACA ACAGCTTGGA GAGGGAGAGG
    CAGAATTTAC CAGCTCGTCC TAGCAGGGCT CCTGTGAGTA TTTGTGGTGG TGGGGAAAAC
    ACCCCAAAGA GTGCCGAGGA ACCGGTGGTG AGACCCAAAG TCCGGAATGT GGCGACTCCA
    AACTGCATGA AACCCAAAGT GTTTTTTGAT ACTGATGATG ATGATGATGT ACCACACAGT
    ACTTCCAGGT GGCGGGATGC TGCCGATGCT GAGGAGGCTC ATGCCGAGGG CCTAGCCAGA
    AGAGGCCGAG GTGAGGCTGC CAGCAGCTCA GAGCCAAGGT ATGCTGAAGA CCAGGATGCC
    AGGAGTGAAC AAGCGAAGGC AGACAAAGTG CCAAGACGGC GGCGAACCAT GGCAGACCCT
    GACTTCTGGG CATACACCGA CGATTACTAC CGATACTACG AGGAAGATTC TGACAGCGAC
    AAAGAGTGGA TGGCTGCCCT GCGCAGGAAG TACCGAAGCC GAGAGCAACC CCAGTCCTCC
    AGCGGAGAAA GCTGGGAGCT TCTGCCAGGA AAGGAAGAAC TGGAACGTCA GCAAGCCGGA
    GCTGGGAGCC TCGCCAGTGC TGGCAGCAAT GGCAGTGGTT ATCCTGAAGA AGTACAAGAC
    CCATCTCTTC AGGAGGAAGA ACAGGCCTCT CTGGAAGAAG GAGAAATCCC TTGGCTTCGC
    TACAATGAGA ATGAAAGCAG CAGCGAGGGT GATAATGAGT CTACCCATGA GCTCATACAG
    CCTGGGATGT TCATGCTGGA TGGAAACAAC AACCTGGAAG ATGACTCCAG CGTGAGCGAA
    GACCTCGAAG TGGACTGGAG CCTGTTTGAT GGGTTTGCCG ATGGCTTGGG AGTGGCCGAA
    GCCATCTCCT ACGTGGATCC TCAGTTCCTC ACCTACATGG CTCTGGAAGA GCGTCTGGCC
    CAGGCAATGG AGACGGCCCT GGCACACTTG GAGTCTCTCG CCGTTGATGT CGAAGTGGCC
    AACCCACCAG CAAGCAAGGA GAGCATTGAT GCCCTTCCTG AGATCCTGGT CACCGAAGAT
    CATGGTGCAG TGGGCCAGGA AATGTGCTGT CCTATCTGCT GCAGCGAATA TGTGAAGGGG
    GAGGTGGCAA CTGAGCTACC ATGCCACCAC TATTTCCACA AGCCCTGCGT GTCCATCTGG
    CTTCAGAAGT CTGGCACCTG CCCAGTGTGC CGCTGCATGT TCCCTCCCCC GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011245847.1
    Translation

    Target ORF information:

    RefSeq Version XM_011247545.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus praja ring finger 1, E3 ubiquitin protein ligase (Pja1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011247545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGG
    TATCCTCCAA GAGAGTACAG GGCCTCGGGG AGCCGAAGAG GATTGGCTTA TGGACACATT
    GACACTGTAG TGGCTCGTGA TAGTGAGGAG GAGGGGGCTG GGCCTGTTGA CCGACTGCCA
    GTGAGAGGGA AAGCTGGCAA GTTTAAGGAT GATCCCGAGA AGGGGGCAAG GTCTTCCCGC
    TTTACTAGTG TTAACCATGA TGCGAAGGAA GAGTGTGGCA AGGTAGAATC ACCCCCTGCA
    GCGAGGTGCT CGGCTCGCAG AGCTGAGCTC TCGAAGCAGA ATGGCTCCTC GGCCTCTCAG
    ATTTCTTCTG CTGAAGGCAG GGCAGCTGCA AAAGGTAACA ACAGCTTGGA GAGGGAGAGG
    CAGAATTTAC CAGCTCGTCC TAGCAGGGCT CCTGTGAGTA TTTGTGGTGG TGGGGAAAAC
    ACCCCAAAGA GTGCCGAGGA ACCGGTGGTG AGACCCAAAG TCCGGAATGT GGCGACTCCA
    AACTGCATGA AACCCAAAGT GTTTTTTGAT ACTGATGATG ATGATGATGT ACCACACAGT
    ACTTCCAGGT GGCGGGATGC TGCCGATGCT GAGGAGGCTC ATGCCGAGGG CCTAGCCAGA
    AGAGGCCGAG GTGAGGCTGC CAGCAGCTCA GAGCCAAGGT ATGCTGAAGA CCAGGATGCC
    AGGAGTGAAC AAGCGAAGGC AGACAAAGTG CCAAGACGGC GGCGAACCAT GGCAGACCCT
    GACTTCTGGG CATACACCGA CGATTACTAC CGATACTACG AGGAAGATTC TGACAGCGAC
    AAAGAGTGGA TGGCTGCCCT GCGCAGGAAG TACCGAAGCC GAGAGCAACC CCAGTCCTCC
    AGCGGAGAAA GCTGGGAGCT TCTGCCAGGA AAGGAAGAAC TGGAACGTCA GCAAGCCGGA
    GCTGGGAGCC TCGCCAGTGC TGGCAGCAAT GGCAGTGGTT ATCCTGAAGA AGTACAAGAC
    CCATCTCTTC AGGAGGAAGA ACAGGCCTCT CTGGAAGAAG GAGAAATCCC TTGGCTTCGC
    TACAATGAGA ATGAAAGCAG CAGCGAGGGT GATAATGAGT CTACCCATGA GCTCATACAG
    CCTGGGATGT TCATGCTGGA TGGAAACAAC AACCTGGAAG ATGACTCCAG CGTGAGCGAA
    GACCTCGAAG TGGACTGGAG CCTGTTTGAT GGGTTTGCCG ATGGCTTGGG AGTGGCCGAA
    GCCATCTCCT ACGTGGATCC TCAGTTCCTC ACCTACATGG CTCTGGAAGA GCGTCTGGCC
    CAGGCAATGG AGACGGCCCT GGCACACTTG GAGTCTCTCG CCGTTGATGT CGAAGTGGCC
    AACCCACCAG CAAGCAAGGA GAGCATTGAT GCCCTTCCTG AGATCCTGGT CACCGAAGAT
    CATGGTGCAG TGGGCCAGGA AATGTGCTGT CCTATCTGCT GCAGCGAATA TGTGAAGGGG
    GAGGTGGCAA CTGAGCTACC ATGCCACCAC TATTTCCACA AGCCCTGCGT GTCCATCTGG
    CTTCAGAAGT CTGGCACCTG CCCAGTGTGC CGCTGCATGT TCCCTCCCCC GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23408
    Clone ID Related Accession (Same CDS sequence) XM_011247545.1 , XM_030251255.1 , NM_001083110.2
    Accession Version XM_030251255.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase Praja-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGG
    TATCCTCCAA GAGAGTACAG GGCCTCGGGG AGCCGAAGAG GATTGGCTTA TGGACACATT
    GACACTGTAG TGGCTCGTGA TAGTGAGGAG GAGGGGGCTG GGCCTGTTGA CCGACTGCCA
    GTGAGAGGGA AAGCTGGCAA GTTTAAGGAT GATCCCGAGA AGGGGGCAAG GTCTTCCCGC
    TTTACTAGTG TTAACCATGA TGCGAAGGAA GAGTGTGGCA AGGTAGAATC ACCCCCTGCA
    GCGAGGTGCT CGGCTCGCAG AGCTGAGCTC TCGAAGCAGA ATGGCTCCTC GGCCTCTCAG
    ATTTCTTCTG CTGAAGGCAG GGCAGCTGCA AAAGGTAACA ACAGCTTGGA GAGGGAGAGG
    CAGAATTTAC CAGCTCGTCC TAGCAGGGCT CCTGTGAGTA TTTGTGGTGG TGGGGAAAAC
    ACCCCAAAGA GTGCCGAGGA ACCGGTGGTG AGACCCAAAG TCCGGAATGT GGCGACTCCA
    AACTGCATGA AACCCAAAGT GTTTTTTGAT ACTGATGATG ATGATGATGT ACCACACAGT
    ACTTCCAGGT GGCGGGATGC TGCCGATGCT GAGGAGGCTC ATGCCGAGGG CCTAGCCAGA
    AGAGGCCGAG GTGAGGCTGC CAGCAGCTCA GAGCCAAGGT ATGCTGAAGA CCAGGATGCC
    AGGAGTGAAC AAGCGAAGGC AGACAAAGTG CCAAGACGGC GGCGAACCAT GGCAGACCCT
    GACTTCTGGG CATACACCGA CGATTACTAC CGATACTACG AGGAAGATTC TGACAGCGAC
    AAAGAGTGGA TGGCTGCCCT GCGCAGGAAG TACCGAAGCC GAGAGCAACC CCAGTCCTCC
    AGCGGAGAAA GCTGGGAGCT TCTGCCAGGA AAGGAAGAAC TGGAACGTCA GCAAGCCGGA
    GCTGGGAGCC TCGCCAGTGC TGGCAGCAAT GGCAGTGGTT ATCCTGAAGA AGTACAAGAC
    CCATCTCTTC AGGAGGAAGA ACAGGCCTCT CTGGAAGAAG GAGAAATCCC TTGGCTTCGC
    TACAATGAGA ATGAAAGCAG CAGCGAGGGT GATAATGAGT CTACCCATGA GCTCATACAG
    CCTGGGATGT TCATGCTGGA TGGAAACAAC AACCTGGAAG ATGACTCCAG CGTGAGCGAA
    GACCTCGAAG TGGACTGGAG CCTGTTTGAT GGGTTTGCCG ATGGCTTGGG AGTGGCCGAA
    GCCATCTCCT ACGTGGATCC TCAGTTCCTC ACCTACATGG CTCTGGAAGA GCGTCTGGCC
    CAGGCAATGG AGACGGCCCT GGCACACTTG GAGTCTCTCG CCGTTGATGT CGAAGTGGCC
    AACCCACCAG CAAGCAAGGA GAGCATTGAT GCCCTTCCTG AGATCCTGGT CACCGAAGAT
    CATGGTGCAG TGGGCCAGGA AATGTGCTGT CCTATCTGCT GCAGCGAATA TGTGAAGGGG
    GAGGTGGCAA CTGAGCTACC ATGCCACCAC TATTTCCACA AGCCCTGCGT GTCCATCTGG
    CTTCAGAAGT CTGGCACCTG CCCAGTGTGC CGCTGCATGT TCCCTCCCCC GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030107115.1
    CDS466..2202
    Translation

    Target ORF information:

    RefSeq Version XM_030251255.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus praja ring finger ubiquitin ligase 1 (Pja1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030251255.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAGCCACC AGGAAAGGAT TGCCAGCCAG AGGAGGACAA CAGCCGAAGT CCCAATGCAC 
    AGATCAACTG CCAATCAAAG CAAGAGGAGC CGGTCACCAT TTGCCAGCAC ACGTCGTCGC
    TGGGATGACA GCGAGAGCTC GGGAGCCAGC CTGGCTGTTG AGAGTGAGGA TTATTCCAGG
    TATCCTCCAA GAGAGTACAG GGCCTCGGGG AGCCGAAGAG GATTGGCTTA TGGACACATT
    GACACTGTAG TGGCTCGTGA TAGTGAGGAG GAGGGGGCTG GGCCTGTTGA CCGACTGCCA
    GTGAGAGGGA AAGCTGGCAA GTTTAAGGAT GATCCCGAGA AGGGGGCAAG GTCTTCCCGC
    TTTACTAGTG TTAACCATGA TGCGAAGGAA GAGTGTGGCA AGGTAGAATC ACCCCCTGCA
    GCGAGGTGCT CGGCTCGCAG AGCTGAGCTC TCGAAGCAGA ATGGCTCCTC GGCCTCTCAG
    ATTTCTTCTG CTGAAGGCAG GGCAGCTGCA AAAGGTAACA ACAGCTTGGA GAGGGAGAGG
    CAGAATTTAC CAGCTCGTCC TAGCAGGGCT CCTGTGAGTA TTTGTGGTGG TGGGGAAAAC
    ACCCCAAAGA GTGCCGAGGA ACCGGTGGTG AGACCCAAAG TCCGGAATGT GGCGACTCCA
    AACTGCATGA AACCCAAAGT GTTTTTTGAT ACTGATGATG ATGATGATGT ACCACACAGT
    ACTTCCAGGT GGCGGGATGC TGCCGATGCT GAGGAGGCTC ATGCCGAGGG CCTAGCCAGA
    AGAGGCCGAG GTGAGGCTGC CAGCAGCTCA GAGCCAAGGT ATGCTGAAGA CCAGGATGCC
    AGGAGTGAAC AAGCGAAGGC AGACAAAGTG CCAAGACGGC GGCGAACCAT GGCAGACCCT
    GACTTCTGGG CATACACCGA CGATTACTAC CGATACTACG AGGAAGATTC TGACAGCGAC
    AAAGAGTGGA TGGCTGCCCT GCGCAGGAAG TACCGAAGCC GAGAGCAACC CCAGTCCTCC
    AGCGGAGAAA GCTGGGAGCT TCTGCCAGGA AAGGAAGAAC TGGAACGTCA GCAAGCCGGA
    GCTGGGAGCC TCGCCAGTGC TGGCAGCAAT GGCAGTGGTT ATCCTGAAGA AGTACAAGAC
    CCATCTCTTC AGGAGGAAGA ACAGGCCTCT CTGGAAGAAG GAGAAATCCC TTGGCTTCGC
    TACAATGAGA ATGAAAGCAG CAGCGAGGGT GATAATGAGT CTACCCATGA GCTCATACAG
    CCTGGGATGT TCATGCTGGA TGGAAACAAC AACCTGGAAG ATGACTCCAG CGTGAGCGAA
    GACCTCGAAG TGGACTGGAG CCTGTTTGAT GGGTTTGCCG ATGGCTTGGG AGTGGCCGAA
    GCCATCTCCT ACGTGGATCC TCAGTTCCTC ACCTACATGG CTCTGGAAGA GCGTCTGGCC
    CAGGCAATGG AGACGGCCCT GGCACACTTG GAGTCTCTCG CCGTTGATGT CGAAGTGGCC
    AACCCACCAG CAAGCAAGGA GAGCATTGAT GCCCTTCCTG AGATCCTGGT CACCGAAGAT
    CATGGTGCAG TGGGCCAGGA AATGTGCTGT CCTATCTGCT GCAGCGAATA TGTGAAGGGG
    GAGGTGGCAA CTGAGCTACC ATGCCACCAC TATTTCCACA AGCCCTGCGT GTCCATCTGG
    CTTCAGAAGT CTGGCACCTG CCCAGTGTGC CGCTGCATGT TCCCTCCCCC GCTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.