KIAA1549 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KIAA1549
Entrez Gene ID	57670
Full Name	KIAA1549
General protein information	Preferred Names KIAA1549 Names UPF0606 protein KIAA1549
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q34
Summary	The protein encoded by this gene belongs to the UPF0606 family. This gene has been found to be fused to the BRAF oncogene in many cases of pilocytic astrocytoma. The fusion results from 2Mb tandem duplications at 7q34. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012].
Disorder MIM:	613344

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164665.2	NP_001158137.1	UPF0606 protein KIAA1549 isoform 2
XM_011516442.2	XP_011514744.1	UPF0606 protein KIAA1549 isoform X1
NM_020910.3	NP_065961.2	UPF0606 protein KIAA1549 isoform 1
NM_020910.2	NP_065961.2	UPF0606 protein KIAA1549 isoform 1
NM_001164665.1	NP_001158137.1	UPF0606 protein KIAA1549 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIAA1549	NP_065961.2
	LOC710668	XP_001107649.2
Danio rerio (zebrafish)	si:dkeyp-27e10.3	XP_003198292.2
Gallus gallus (chicken)	KIAA1549	XP_003640459.2
Mus musculus (house mouse)	D630045J12Rik	NP_918950.2
Rattus norvegicus (Norway rat)	RGD1306271	XP_006236401.1
Danio rerio (zebrafish)	si:ch211-1e14.1	XP_005159051.1
Pan troglodytes (chimpanzee)	KIAA1549	XP_001149607.2
Canis lupus familiaris (dog)	KIAA1549	XP_005629795.1

Gene Ontology
Bibliography

Related articles in PubMed

Whole Chromosome 7 Gain Predicts Higher Risk of Recurrence in Pediatric Pilocytic Astrocytomas Independently From KIAA1549-BRAF Fusion Status.
Roth JJ, Fierst TM, Waanders AJ, Yimei L, Biegel JA, Santi M
Journal of neuropathology and experimental neurology75(4)306-15(2016 Apr)

Analysis of IDH1-R132 mutation, BRAF V600 mutation and KIAA1549-BRAF fusion transcript status in central nervous system tumors supports pediatric tumor classification.
Gierke M, Sperveslage J, Schwab D, Beschorner R, Ebinger M, Schuhmann MU, Schittenhelm J
Journal of cancer research and clinical oncology142(1)89-100(2016 Jan)

KIAA1549-BRAF fusions and IDH mutations can coexist in diffuse gliomas of adults.
Badiali M, Gleize V, Paris S, Moi L, Elhouadani S, Arcella A, Morace R, Antonelli M, Buttarelli FR, Figarella-Branger D, Kim YH, Ohgaki H, Mokhtari K, Sanson M, Giangaspero F
Brain pathology (Zurich, Switzerland)22(6)841-7(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that when whole chromosome 7 gain accompanies the KIAA1549-BRAF fusion, the fusion likely arises first.
Title: Whole Chromosome 7 Gain Predicts Higher Risk of Recurrence in Pediatric Pilocytic Astrocytomas Independently From KIAA1549-BRAF Fusion Status.

Mutations in BRAF-KIAA1549 were associated with central nervous system tumors.
Title: Analysis of IDH1-R132 mutation, BRAF V600 mutation and KIAA1549-BRAF fusion transcript status in central nervous system tumors supports pediatric tumor classification.

This study confirmed the high frequency of KIAA1549:BRAF fusion in Pilocytic Astrocytomas.
Title: KIAA1549: BRAF Gene Fusion and FGFR1 Hotspot Mutations Are Prognostic Factors in Pilocytic Astrocytomas.

Pediatric oligodendrogliomas (pODGs) can harbor the KIAA1549-BRAF fusion with aberrant MAPK/ERK signaling, and there exists an option of targeting these pathways in such patients.
Title: Oncogenic KIAA1549-BRAF fusion with activation of the MAPK/ERK pathway in pediatric oligodendrogliomas.

we identify KIAA1549-BRAF gene fusions in 45 % of 82 low-grade glioma samples
Title: Analysis of KIAA1549-BRAF fusion gene expression and IDH1/IDH2 mutations in low grade pediatric astrocytomas.

The following KIAA1549 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIAA1549 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01745	NM_020910.3 Latest version!	Homo sapiens KIAA1549 (KIAA1549), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu22824	NM_001164665.2 Latest version!	Homo sapiens KIAA1549 (KIAA1549), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu70837	XM_011516442.2 Latest version!	Homo sapiens KIAA1549 (KIAA1549), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu01745	NM_020910.2	Homo sapiens KIAA1549 (KIAA1549), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu22824	NM_001164665.1	Homo sapiens KIAA1549 (KIAA1549), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIAA1549 ( NM_020910.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065961.2 Homo sapiens KIAA1549 (KIAA1549), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01745
Clone ID Related Accession (Same CDS sequence)	NM_020910.3 , NM_020910.2
Accession Version	NM_020910.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	UPF0606 protein KIAA1549 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC083868.3, AM989468.1 and AC018663.3. On Jun 2, 2019 this sequence version replaced NM_020910.2. Transcript Variant: This variant (1), also known as the long-form, v1, uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. The resulting isoform (1) lacks an internal segment near the C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGGTACGGT GTTGATCACT 
GACGCGTACC TGCCATCAGG ATCCTCGTTT GTTTCTGAAG CAACCCCCTT CCCTCTGCCC 
ACAGAGCTGA CCGTCGTGGG CCCATCACTC ACACCCACAG AGGTGCCACT GAACACCTCC 
ACGGAAGTGA GCACAACCAG CACCGGTGCT GCCACTGGTG GTCCCCTCGA CTCCACCCTG 
ATGGGTGACG CCGCAAGTCA GAGCCCCCCA GAGAGTAGTG CTGCTCCTCC CCTGCCATCC 
CTGCGTCCCG TGACTGCCTT CACTCTCGAA GCAACAGTCG ACACACCAAC ACTGGCTACT 
GCCAAGCCGC CATATGTTTG TGATATCACA GTCCCCGATG CCTATCTGAT CACAACTGTG 
CTGGCCAGAA GAGCTGTGCA GGAGTACATC ATTACAGCAA TCAAAGAAGT ACTGAGGATT 
CACTTCAACC GTGCAGTGGA ACTGAAGGTT TACGAACTAT TTACTGACTT CACTTTTCTG 
GTAACATCCG GTCCTTTCGT TTACACGGCA ATATCCGTCA TAAATGTGCT TATAAACAGT 
AAGCTTGTCC GTGACCAGAC TCCTTTAATC CTGTCTGTGA AACCTTCTTT CCTTGTGCCA 
GAGTCCAGGT TCCAAGTTCA AACAGTACTT CAGTTTGTGC CTCCGAGTGT GGATACTGGC 
TTCTGCAACT TCACCCAGCG CATTGAGAAA GGCCTAATGA CAGCTCTCTT TGAAGTGAGA 
AAACACCACC AGGGAACGTA TAACCTCACG GTGCAGATCT TGAATATCAC CATCAGTTCC 
TCAAGGGTGA CTCCTCGGCG GGGCCCGGTG AATATCATCT TTGCGGTTAA AAGCACACAG 
GGATTTTTGA ATGGGTCGGA AGTGAGCGAG CTGCTCAGAA ACTTGAGTGT GGTGGAGTTC 
AGTTTCTATC TGGGATACCC AGTGCTGCAG ATCGCAGAGC CCTTCCAGTA TCCACAGCTC 
AACTTATCTC AGTTGCTGAA GTCCTCTTGG GTCAGAACAG TTCTCCTGGG CGTCATGGAG 
AAGCAACTCC AGAATGAAGT GTTTCAAGCC GAGATGGAAC GCAAGCTGGC CCAGCTGCTC 
AGCGAGGTTT CCACCAGAAG GCGGATGTGG AGAAGGGCCA CTGTAGCTGC AGGGAACAGT 
GTGGTGCAGG TGGTAAATGT GTCGAGGCTG GAGGGAGATG ACAATCCGGT ACAGCTCATC 
TACTTTGTGG AGGATCAAGA TGGAGAAAGA CTCAGTGCAG TCAAGTCTTC GGACCTGATT 
AACAAAATGG ACCTCCAGAG AGCAGCCATC ATCTTGGGTT ACCGAATTCA AGGTGTCATT 
GCCCAGCCTG TCGACAGGGT GAAGAGGCCG TCTCCGGAAT CCCAGAGCAA CAACTTGTGG 
GTCATTGTTG GCGTGGTCAT CCCAGTGCTG GTGGTGATGG TGATTGTTGT CATCCTCTAC 
TGGAAACTAT GCCGCACAGA CAAGCTAGAC TTTCAGCCTG ACACTGTGGC CAACATTCAG 
CAGCGTCAGA AGCTGCAGAT CCCTAGTGTG AAGGGCTTCG ATTTTGCTAA GCAGCATCTG 
GGTCAGCACA ATAAAGACGA CATATTGATT ATTCATGAGC CAGCGCCACT GCCAGGACCT 
CTGAAGGACC ACACCACGCC CTCGGAAAAT GGAGACGTGC CAAGCCCCAA GTCAAAGATC 
CCTTCCAAGA ATGTTCGTCA CAGAGGAAGA GTTTCTCCCT CAGATGCTGA CTCTACGGTC 
AGTGAAGAGT CCAGCGAGAG GGACGCAGGA GATAAGACGC CGGGAGCCGT CAACGATGGC 
AGGTCCCACA GAGCTCCGCA GAGCGGGCCA CCACTGCCCA GTTCGGGAAA TGAGCAGCAC 
TCATCAGCCT CCATCTTCGA GCACGTGGAC AGGATCTCCC GCCCCCCGGA GGCTAGCCGG 
CGGGTCCCCA GTAAGATCCA GCTTATCGCC ATGCAGCCGA TCCCGGCACC TCCCGTCCAG 
CGCCCCTCCC CAGCCGACCG AGTGGCGGAA AGCAATAAAA TCAACAAAGA GATTCAGACC 
GCGCTGCGGC ACAAGTCTGA GATCGAGCAC CATCGCAACA AGATCCGCCT GCGCGCCAAG 
CGCCGCGGGC ACTACGAGTT CCCGGTGGTA GACGACCTGT CCTCGGGCGA CACTAAGGAG 
CGACACCGGG TGTACCGCAG GGCACAGATG CAGATCGACA AGATCCTGGA CCCCACGGCC 
AGCGTGCCCT CCGTGTTCAT AGAGCCCAGG AAGAGCTCAC GGATAAAACG TTCTCCCAAG 
CCTCGCCGGA AACACCAGGT CAACGGCTGT CCTGCCGACG CTGAGAAGGA CCGGCTCATC 
ACCACAGACA GCGATGGCAC CTACAGGAGG CCCCCCGGCG TCCACAACTC AGCCTACATC 
GGATGCCCAT CGGATCCTGA CCTCCCAGCC GATGTGCAGA CACCATCCTC GGTGGAACTG 
GGGAGGTATC CAGCCCTTCC CTTCCCGGCC TCCCAGTACA TCCCACCCCA GCCGTCCATC 
GAGGAGGCAC GCCAGACCAT GCACTCCCTC CTGGACGACG CCTTTGCCCT CGTGGCCCCC 
AGCAGCCAGC CTGCCAGCAC CGCAGGTGTA GGCCCCGGAG TCCCACCCGG CCTGCCCGCA 
AACAGCACCC CTTCCCAGGA AGAGAGGCGA GCCACCCAGT GGGGGTCCTT CTACAGCCCA 
GCCCAGACGG CCAACAATCC CTGCAGTAGA TACGAAGACT ATGGAATGAC TCCCCCGACG 
GGTCCATTGC CAAGACCAGG TTTTGGCCCC GGTTTGCTGC AGTCTACAGA GCTGGTGCCC 
CCTGACCCTC AGCAGCCACA GGCCTCCGCC GAAGCCCCAT TTGCTGCCAG AGGGATCTAC 
TCGGAGGAGA TGCCGTCGGT GGCCCGGCCT CGGCCTGTCG GGGGTACCAC AGGCTCCCAG 
ATCCAGCACC TGACACAGGT GGGGATTGCC AGCAGAATTG GAGCTCAGCC AGTGGAAATC 
CCGCCAAGCA GAGGCAGCCA GTATGGGGGG CCAGGCTGGC CTTCGTACGG GGAGGACGAA 
GCGGGGCGAA GAGAGGCCGT GCCAAGGACT TCAGGCAGGG AGCCCTCAGC TCCTTCCGGG 
AACCTCCCCC ACCGGGGACT GCAGGGCCCT GGGCTGGGTT ACCCCACCAG CTCCACGGAA 
GACCTCCAGC CTGGCCACTC CTCGGCCTCT CTCATCAAAG CAATCCGCGA GGAGCTCCTC 
CGGCTCTCCC AGAAACAGAG CACCGTGCAG AACTTCCACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065961.2
CDS	121..5925
Translation	MPGARRRRRGAAMEGKPRAGVALAPGPSGRRPSARCARRRRPGLLLPGLWLLLLARPASCAPDELSPEQHNLSLYSMELVLKKSTGHSAAQVALTETAPGSQHSSPLHVTAPPSATTFDTAFFNQGKQTKSTADPSIFVATYVSVTSKEVAVNDDEMDNFLPDTHWTTPRMVSPIQYITVSPPGLPREALEPMLTPSLPMVSLQDEEVTSGWQNTTRQPAAYAESASHFHTFRSAFRTSEGIVPTPGRNLVLYPTDAYSHLSSRTLPEIVASLTEGVETTLFLSSRSLMPQPLGDGITIPLPSLGEVSQPPEEVWATSADRYTDVTTVLSQSLEETISPRTYPTVTASHAALAFSRTHSPLLSTPLAFASSASPTDVSSNPFLPSDSSKTSELHSNSALPGPVDNTHILSPVSSFRPYTWCAACTVPSPQQVLATSLMEKDVGSGDGAETLCMTVLEESSISLMSSVVADFSEFEEDPQVFNTLFPSRPIVPLSSRSMEISETSVGISAEVDMSSVTTTQVPPAHGRLSVPASLDPTAGSLSVAETQVTPSSVTTAFFSVITSILLDSSFSVIANKNTPSLAVRDPSVFTPYSLVPSVESSLFSDQERSSFSEHKPRGALDFASSFFSTPPLELSGSISSPSEAPASLSLMPSDLSPFTSQSFSPLVETFTLFDSSDLQSSQLSLPSSTNLEFSQLQPSSELPLNTIMLLPSRSEVSPWSSFPSDSLEFVEASTVSLTDSEAHFTSAFIETTSYLESSLISHESAVTALVPPGSESFDILTAGIQATSPLTTVHTTPILTESSLFSTLTPPDDQISALDGHVSVLASFSKAIPTGTVLITDAYLPSGSSFVSEATPFPLPTELTVVGPSLTPTEVPLNTSTEVSTTSTGAATGGPLDSTLMGDAASQSPPESSAAPPLPSLRPVTAFTLEATVDTPTLATAKPPYVCDITVPDAYLITTVLARRAVQEYIITAIKEVLRIHFNRAVELKVYELFTDFTFLVTSGPFVYTAISVINVLINSKLVRDQTPLILSVKPSFLVPESRFQVQTVLQFVPPSVDTGFCNFTQRIEKGLMTALFEVRKHHQGTYNLTVQILNITISSSRVTPRRGPVNIIFAVKSTQGFLNGSEVSELLRNLSVVEFSFYLGYPVLQIAEPFQYPQLNLSQLLKSSWVRTVLLGVMEKQLQNEVFQAEMERKLAQLLSEVSTRRRMWRRATVAAGNSVVQVVNVSRLEGDDNPVQLIYFVEDQDGERLSAVKSSDLINKMDLQRAAIILGYRIQGVIAQPVDRVKRPSPESQSNNLWVIVGVVIPVLVVMVIVVILYWKLCRTDKLDFQPDTVANIQQRQKLQIPSVKGFDFAKQHLGQHNKDDILIIHEPAPLPGPLKDHTTPSENGDVPSPKSKIPSKNVRHRGRVSPSDADSTVSEESSERDAGDKTPGAVNDGRSHRAPQSGPPLPSSGNEQHSSASIFEHVDRISRPPEASRRVPSKIQLIAMQPIPAPPVQRPSPADRVAESNKINKEIQTALRHKSEIEHHRNKIRLRAKRRGHYEFPVVDDLSSGDTKERHRVYRRAQMQIDKILDPTASVPSVFIEPRKSSRIKRSPKPRRKHQVNGCPADAEKDRLITTDSDGTYRRPPGVHNSAYIGCPSDPDLPADVQTPSSVELGRYPALPFPASQYIPPQPSIEEARQTMHSLLDDAFALVAPSSQPASTAGVGPGVPPGLPANSTPSQEERRATQWGSFYSPAQTANNPCSRYEDYGMTPPTGPLPRPGFGPGLLQSTELVPPDPQQPQASAEAPFAARGIYSEEMPSVARPRPVGGTTGSQIQHLTQVGIASRIGAQPVEIPPSRGSQYGGPGWPSYGEDEAGRREAVPRTSGREPSAPSGNLPHRGLQGPGLGYPTSSTEDLQPGHSSASLIKAIREELLRLSQKQSTVQNFHS

Target ORF information:

RefSeq Version	NM_020910.3
Organism	Homo sapiens(human)
Definition	Homo sapiens KIAA1549 (KIAA1549), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020910.3

ORF Insert Sequence:

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGGTACGGT GTTGATCACT 
GACGCGTACC TGCCATCAGG ATCCTCGTTT GTTTCTGAAG CAACCCCCTT CCCTCTGCCC 
ACAGAGCTGA CCGTCGTGGG CCCATCACTC ACACCCACAG AGGTGCCACT GAACACCTCC 
ACGGAAGTGA GCACAACCAG CACCGGTGCT GCCACTGGTG GTCCCCTCGA CTCCACCCTG 
ATGGGTGACG CCGCAAGTCA GAGCCCCCCA GAGAGTAGTG CTGCTCCTCC CCTGCCATCC 
CTGCGTCCCG TGACTGCCTT CACTCTCGAA GCAACAGTCG ACACACCAAC ACTGGCTACT 
GCCAAGCCGC CATATGTTTG TGATATCACA GTCCCCGATG CCTATCTGAT CACAACTGTG 
CTGGCCAGAA GAGCTGTGCA GGAGTACATC ATTACAGCAA TCAAAGAAGT ACTGAGGATT 
CACTTCAACC GTGCAGTGGA ACTGAAGGTT TACGAACTAT TTACTGACTT CACTTTTCTG 
GTAACATCCG GTCCTTTCGT TTACACGGCA ATATCCGTCA TAAATGTGCT TATAAACAGT 
AAGCTTGTCC GTGACCAGAC TCCTTTAATC CTGTCTGTGA AACCTTCTTT CCTTGTGCCA 
GAGTCCAGGT TCCAAGTTCA AACAGTACTT CAGTTTGTGC CTCCGAGTGT GGATACTGGC 
TTCTGCAACT TCACCCAGCG CATTGAGAAA GGCCTAATGA CAGCTCTCTT TGAAGTGAGA 
AAACACCACC AGGGAACGTA TAACCTCACG GTGCAGATCT TGAATATCAC CATCAGTTCC 
TCAAGGGTGA CTCCTCGGCG GGGCCCGGTG AATATCATCT TTGCGGTTAA AAGCACACAG 
GGATTTTTGA ATGGGTCGGA AGTGAGCGAG CTGCTCAGAA ACTTGAGTGT GGTGGAGTTC 
AGTTTCTATC TGGGATACCC AGTGCTGCAG ATCGCAGAGC CCTTCCAGTA TCCACAGCTC 
AACTTATCTC AGTTGCTGAA GTCCTCTTGG GTCAGAACAG TTCTCCTGGG CGTCATGGAG 
AAGCAACTCC AGAATGAAGT GTTTCAAGCC GAGATGGAAC GCAAGCTGGC CCAGCTGCTC 
AGCGAGGTTT CCACCAGAAG GCGGATGTGG AGAAGGGCCA CTGTAGCTGC AGGGAACAGT 
GTGGTGCAGG TGGTAAATGT GTCGAGGCTG GAGGGAGATG ACAATCCGGT ACAGCTCATC 
TACTTTGTGG AGGATCAAGA TGGAGAAAGA CTCAGTGCAG TCAAGTCTTC GGACCTGATT 
AACAAAATGG ACCTCCAGAG AGCAGCCATC ATCTTGGGTT ACCGAATTCA AGGTGTCATT 
GCCCAGCCTG TCGACAGGGT GAAGAGGCCG TCTCCGGAAT CCCAGAGCAA CAACTTGTGG 
GTCATTGTTG GCGTGGTCAT CCCAGTGCTG GTGGTGATGG TGATTGTTGT CATCCTCTAC 
TGGAAACTAT GCCGCACAGA CAAGCTAGAC TTTCAGCCTG ACACTGTGGC CAACATTCAG 
CAGCGTCAGA AGCTGCAGAT CCCTAGTGTG AAGGGCTTCG ATTTTGCTAA GCAGCATCTG 
GGTCAGCACA ATAAAGACGA CATATTGATT ATTCATGAGC CAGCGCCACT GCCAGGACCT 
CTGAAGGACC ACACCACGCC CTCGGAAAAT GGAGACGTGC CAAGCCCCAA GTCAAAGATC 
CCTTCCAAGA ATGTTCGTCA CAGAGGAAGA GTTTCTCCCT CAGATGCTGA CTCTACGGTC 
AGTGAAGAGT CCAGCGAGAG GGACGCAGGA GATAAGACGC CGGGAGCCGT CAACGATGGC 
AGGTCCCACA GAGCTCCGCA GAGCGGGCCA CCACTGCCCA GTTCGGGAAA TGAGCAGCAC 
TCATCAGCCT CCATCTTCGA GCACGTGGAC AGGATCTCCC GCCCCCCGGA GGCTAGCCGG 
CGGGTCCCCA GTAAGATCCA GCTTATCGCC ATGCAGCCGA TCCCGGCACC TCCCGTCCAG 
CGCCCCTCCC CAGCCGACCG AGTGGCGGAA AGCAATAAAA TCAACAAAGA GATTCAGACC 
GCGCTGCGGC ACAAGTCTGA GATCGAGCAC CATCGCAACA AGATCCGCCT GCGCGCCAAG 
CGCCGCGGGC ACTACGAGTT CCCGGTGGTA GACGACCTGT CCTCGGGCGA CACTAAGGAG 
CGACACCGGG TGTACCGCAG GGCACAGATG CAGATCGACA AGATCCTGGA CCCCACGGCC 
AGCGTGCCCT CCGTGTTCAT AGAGCCCAGG AAGAGCTCAC GGATAAAACG TTCTCCCAAG 
CCTCGCCGGA AACACCAGGT CAACGGCTGT CCTGCCGACG CTGAGAAGGA CCGGCTCATC 
ACCACAGACA GCGATGGCAC CTACAGGAGG CCCCCCGGCG TCCACAACTC AGCCTACATC 
GGATGCCCAT CGGATCCTGA CCTCCCAGCC GATGTGCAGA CACCATCCTC GGTGGAACTG 
GGGAGGTATC CAGCCCTTCC CTTCCCGGCC TCCCAGTACA TCCCACCCCA GCCGTCCATC 
GAGGAGGCAC GCCAGACCAT GCACTCCCTC CTGGACGACG CCTTTGCCCT CGTGGCCCCC 
AGCAGCCAGC CTGCCAGCAC CGCAGGTGTA GGCCCCGGAG TCCCACCCGG CCTGCCCGCA 
AACAGCACCC CTTCCCAGGA AGAGAGGCGA GCCACCCAGT GGGGGTCCTT CTACAGCCCA 
GCCCAGACGG CCAACAATCC CTGCAGTAGA TACGAAGACT ATGGAATGAC TCCCCCGACG 
GGTCCATTGC CAAGACCAGG TTTTGGCCCC GGTTTGCTGC AGTCTACAGA GCTGGTGCCC 
CCTGACCCTC AGCAGCCACA GGCCTCCGCC GAAGCCCCAT TTGCTGCCAG AGGGATCTAC 
TCGGAGGAGA TGCCGTCGGT GGCCCGGCCT CGGCCTGTCG GGGGTACCAC AGGCTCCCAG 
ATCCAGCACC TGACACAGGT GGGGATTGCC AGCAGAATTG GAGCTCAGCC AGTGGAAATC 
CCGCCAAGCA GAGGCAGCCA GTATGGGGGG CCAGGCTGGC CTTCGTACGG GGAGGACGAA 
GCGGGGCGAA GAGAGGCCGT GCCAAGGACT TCAGGCAGGG AGCCCTCAGC TCCTTCCGGG 
AACCTCCCCC ACCGGGGACT GCAGGGCCCT GGGCTGGGTT ACCCCACCAG CTCCACGGAA 
GACCTCCAGC CTGGCCACTC CTCGGCCTCT CTCATCAAAG CAATCCGCGA GGAGCTCCTC 
CGGCTCTCCC AGAAACAGAG CACCGTGCAG AACTTCCACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1549 ( NM_020910.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065961.2 Homo sapiens KIAA1549 (KIAA1549), transcript variant 1, mRNA.

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CloneID	OHu01745
Clone ID Related Accession (Same CDS sequence)	NM_020910.3 , NM_020910.2
Accession Version	NM_020910.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	UPF0606 protein KIAA1549 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AM989468.1 and AC018663.3. On Sep 9, 2009 this sequence version replaced NM_020910.1. Transcript Variant: This variant (1), also known as the long-form, v1, uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. The resulting isoform (1) lacks an internal segment near the C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AM989468.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGGTACGGT GTTGATCACT 
GACGCGTACC TGCCATCAGG ATCCTCGTTT GTTTCTGAAG CAACCCCCTT CCCTCTGCCC 
ACAGAGCTGA CCGTCGTGGG CCCATCACTC ACACCCACAG AGGTGCCACT GAACACCTCC 
ACGGAAGTGA GCACAACCAG CACCGGTGCT GCCACTGGTG GTCCCCTCGA CTCCACCCTG 
ATGGGTGACG CCGCAAGTCA GAGCCCCCCA GAGAGTAGTG CTGCTCCTCC CCTGCCATCC 
CTGCGTCCCG TGACTGCCTT CACTCTCGAA GCAACAGTCG ACACACCAAC ACTGGCTACT 
GCCAAGCCGC CATATGTTTG TGATATCACA GTCCCCGATG CCTATCTGAT CACAACTGTG 
CTGGCCAGAA GAGCTGTGCA GGAGTACATC ATTACAGCAA TCAAAGAAGT ACTGAGGATT 
CACTTCAACC GTGCAGTGGA ACTGAAGGTT TACGAACTAT TTACTGACTT CACTTTTCTG 
GTAACATCCG GTCCTTTCGT TTACACGGCA ATATCCGTCA TAAATGTGCT TATAAACAGT 
AAGCTTGTCC GTGACCAGAC TCCTTTAATC CTGTCTGTGA AACCTTCTTT CCTTGTGCCA 
GAGTCCAGGT TCCAAGTTCA AACAGTACTT CAGTTTGTGC CTCCGAGTGT GGATACTGGC 
TTCTGCAACT TCACCCAGCG CATTGAGAAA GGCCTAATGA CAGCTCTCTT TGAAGTGAGA 
AAACACCACC AGGGAACGTA TAACCTCACG GTGCAGATCT TGAATATCAC CATCAGTTCC 
TCAAGGGTGA CTCCTCGGCG GGGCCCGGTG AATATCATCT TTGCGGTTAA AAGCACACAG 
GGATTTTTGA ATGGGTCGGA AGTGAGCGAG CTGCTCAGAA ACTTGAGTGT GGTGGAGTTC 
AGTTTCTATC TGGGATACCC AGTGCTGCAG ATCGCAGAGC CCTTCCAGTA TCCACAGCTC 
AACTTATCTC AGTTGCTGAA GTCCTCTTGG GTCAGAACAG TTCTCCTGGG CGTCATGGAG 
AAGCAACTCC AGAATGAAGT GTTTCAAGCC GAGATGGAAC GCAAGCTGGC CCAGCTGCTC 
AGCGAGGTTT CCACCAGAAG GCGGATGTGG AGAAGGGCCA CTGTAGCTGC AGGGAACAGT 
GTGGTGCAGG TGGTAAATGT GTCGAGGCTG GAGGGAGATG ACAATCCGGT ACAGCTCATC 
TACTTTGTGG AGGATCAAGA TGGAGAAAGA CTCAGTGCAG TCAAGTCTTC GGACCTGATT 
AACAAAATGG ACCTCCAGAG AGCAGCCATC ATCTTGGGTT ACCGAATTCA AGGTGTCATT 
GCCCAGCCTG TCGACAGGGT GAAGAGGCCG TCTCCGGAAT CCCAGAGCAA CAACTTGTGG 
GTCATTGTTG GCGTGGTCAT CCCAGTGCTG GTGGTGATGG TGATTGTTGT CATCCTCTAC 
TGGAAACTAT GCCGCACAGA CAAGCTAGAC TTTCAGCCTG ACACTGTGGC CAACATTCAG 
CAGCGTCAGA AGCTGCAGAT CCCTAGTGTG AAGGGCTTCG ATTTTGCTAA GCAGCATCTG 
GGTCAGCACA ATAAAGACGA CATATTGATT ATTCATGAGC CAGCGCCACT GCCAGGACCT 
CTGAAGGACC ACACCACGCC CTCGGAAAAT GGAGACGTGC CAAGCCCCAA GTCAAAGATC 
CCTTCCAAGA ATGTTCGTCA CAGAGGAAGA GTTTCTCCCT CAGATGCTGA CTCTACGGTC 
AGTGAAGAGT CCAGCGAGAG GGACGCAGGA GATAAGACGC CGGGAGCCGT CAACGATGGC 
AGGTCCCACA GAGCTCCGCA GAGCGGGCCA CCACTGCCCA GTTCGGGAAA TGAGCAGCAC 
TCATCAGCCT CCATCTTCGA GCACGTGGAC AGGATCTCCC GCCCCCCGGA GGCTAGCCGG 
CGGGTCCCCA GTAAGATCCA GCTTATCGCC ATGCAGCCGA TCCCGGCACC TCCCGTCCAG 
CGCCCCTCCC CAGCCGACCG AGTGGCGGAA AGCAATAAAA TCAACAAAGA GATTCAGACC 
GCGCTGCGGC ACAAGTCTGA GATCGAGCAC CATCGCAACA AGATCCGCCT GCGCGCCAAG 
CGCCGCGGGC ACTACGAGTT CCCGGTGGTA GACGACCTGT CCTCGGGCGA CACTAAGGAG 
CGACACCGGG TGTACCGCAG GGCACAGATG CAGATCGACA AGATCCTGGA CCCCACGGCC 
AGCGTGCCCT CCGTGTTCAT AGAGCCCAGG AAGAGCTCAC GGATAAAACG TTCTCCCAAG 
CCTCGCCGGA AACACCAGGT CAACGGCTGT CCTGCCGACG CTGAGAAGGA CCGGCTCATC 
ACCACAGACA GCGATGGCAC CTACAGGAGG CCCCCCGGCG TCCACAACTC AGCCTACATC 
GGATGCCCAT CGGATCCTGA CCTCCCAGCC GATGTGCAGA CACCATCCTC GGTGGAACTG 
GGGAGGTATC CAGCCCTTCC CTTCCCGGCC TCCCAGTACA TCCCACCCCA GCCGTCCATC 
GAGGAGGCAC GCCAGACCAT GCACTCCCTC CTGGACGACG CCTTTGCCCT CGTGGCCCCC 
AGCAGCCAGC CTGCCAGCAC CGCAGGTGTA GGCCCCGGAG TCCCACCCGG CCTGCCCGCA 
AACAGCACCC CTTCCCAGGA AGAGAGGCGA GCCACCCAGT GGGGGTCCTT CTACAGCCCA 
GCCCAGACGG CCAACAATCC CTGCAGTAGA TACGAAGACT ATGGAATGAC TCCCCCGACG 
GGTCCATTGC CAAGACCAGG TTTTGGCCCC GGTTTGCTGC AGTCTACAGA GCTGGTGCCC 
CCTGACCCTC AGCAGCCACA GGCCTCCGCC GAAGCCCCAT TTGCTGCCAG AGGGATCTAC 
TCGGAGGAGA TGCCGTCGGT GGCCCGGCCT CGGCCTGTCG GGGGTACCAC AGGCTCCCAG 
ATCCAGCACC TGACACAGGT GGGGATTGCC AGCAGAATTG GAGCTCAGCC AGTGGAAATC 
CCGCCAAGCA GAGGCAGCCA GTATGGGGGG CCAGGCTGGC CTTCGTACGG GGAGGACGAA 
GCGGGGCGAA GAGAGGCCGT GCCAAGGACT TCAGGCAGGG AGCCCTCAGC TCCTTCCGGG 
AACCTCCCCC ACCGGGGACT GCAGGGCCCT GGGCTGGGTT ACCCCACCAG CTCCACGGAA 
GACCTCCAGC CTGGCCACTC CTCGGCCTCT CTCATCAAAG CAATCCGCGA GGAGCTCCTC 
CGGCTCTCCC AGAAACAGAG CACCGTGCAG AACTTCCACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065961.2
CDS	50..5854
Translation	MPGARRRRRGAAMEGKPRAGVALAPGPSGRRPSARCARRRRPGLLLPGLWLLLLARPASCAPDELSPEQHNLSLYSMELVLKKSTGHSAAQVALTETAPGSQHSSPLHVTAPPSATTFDTAFFNQGKQTKSTADPSIFVATYVSVTSKEVAVNDDEMDNFLPDTHWTTPRMVSPIQYITVSPPGLPREALEPMLTPSLPMVSLQDEEVTSGWQNTTRQPAAYAESASHFHTFRSAFRTSEGIVPTPGRNLVLYPTDAYSHLSSRTLPEIVASLTEGVETTLFLSSRSLMPQPLGDGITIPLPSLGEVSQPPEEVWATSADRYTDVTTVLSQSLEETISPRTYPTVTASHAALAFSRTHSPLLSTPLAFASSASPTDVSSNPFLPSDSSKTSELHSNSALPGPVDNTHILSPVSSFRPYTWCAACTVPSPQQVLATSLMEKDVGSGDGAETLCMTVLEESSISLMSSVVADFSEFEEDPQVFNTLFPSRPIVPLSSRSMEISETSVGISAEVDMSSVTTTQVPPAHGRLSVPASLDPTAGSLSVAETQVTPSSVTTAFFSVITSILLDSSFSVIANKNTPSLAVRDPSVFTPYSLVPSVESSLFSDQERSSFSEHKPRGALDFASSFFSTPPLELSGSISSPSEAPASLSLMPSDLSPFTSQSFSPLVETFTLFDSSDLQSSQLSLPSSTNLEFSQLQPSSELPLNTIMLLPSRSEVSPWSSFPSDSLEFVEASTVSLTDSEAHFTSAFIETTSYLESSLISHESAVTALVPPGSESFDILTAGIQATSPLTTVHTTPILTESSLFSTLTPPDDQISALDGHVSVLASFSKAIPTGTVLITDAYLPSGSSFVSEATPFPLPTELTVVGPSLTPTEVPLNTSTEVSTTSTGAATGGPLDSTLMGDAASQSPPESSAAPPLPSLRPVTAFTLEATVDTPTLATAKPPYVCDITVPDAYLITTVLARRAVQEYIITAIKEVLRIHFNRAVELKVYELFTDFTFLVTSGPFVYTAISVINVLINSKLVRDQTPLILSVKPSFLVPESRFQVQTVLQFVPPSVDTGFCNFTQRIEKGLMTALFEVRKHHQGTYNLTVQILNITISSSRVTPRRGPVNIIFAVKSTQGFLNGSEVSELLRNLSVVEFSFYLGYPVLQIAEPFQYPQLNLSQLLKSSWVRTVLLGVMEKQLQNEVFQAEMERKLAQLLSEVSTRRRMWRRATVAAGNSVVQVVNVSRLEGDDNPVQLIYFVEDQDGERLSAVKSSDLINKMDLQRAAIILGYRIQGVIAQPVDRVKRPSPESQSNNLWVIVGVVIPVLVVMVIVVILYWKLCRTDKLDFQPDTVANIQQRQKLQIPSVKGFDFAKQHLGQHNKDDILIIHEPAPLPGPLKDHTTPSENGDVPSPKSKIPSKNVRHRGRVSPSDADSTVSEESSERDAGDKTPGAVNDGRSHRAPQSGPPLPSSGNEQHSSASIFEHVDRISRPPEASRRVPSKIQLIAMQPIPAPPVQRPSPADRVAESNKINKEIQTALRHKSEIEHHRNKIRLRAKRRGHYEFPVVDDLSSGDTKERHRVYRRAQMQIDKILDPTASVPSVFIEPRKSSRIKRSPKPRRKHQVNGCPADAEKDRLITTDSDGTYRRPPGVHNSAYIGCPSDPDLPADVQTPSSVELGRYPALPFPASQYIPPQPSIEEARQTMHSLLDDAFALVAPSSQPASTAGVGPGVPPGLPANSTPSQEERRATQWGSFYSPAQTANNPCSRYEDYGMTPPTGPLPRPGFGPGLLQSTELVPPDPQQPQASAEAPFAARGIYSEEMPSVARPRPVGGTTGSQIQHLTQVGIASRIGAQPVEIPPSRGSQYGGPGWPSYGEDEAGRREAVPRTSGREPSAPSGNLPHRGLQGPGLGYPTSSTEDLQPGHSSASLIKAIREELLRLSQKQSTVQNFHS

Target ORF information:

RefSeq Version	NM_020910.2
Organism	Homo sapiens(human)
Definition	Homo sapiens KIAA1549 (KIAA1549), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020910.2

ORF Insert Sequence:

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGGTACGGT GTTGATCACT 
GACGCGTACC TGCCATCAGG ATCCTCGTTT GTTTCTGAAG CAACCCCCTT CCCTCTGCCC 
ACAGAGCTGA CCGTCGTGGG CCCATCACTC ACACCCACAG AGGTGCCACT GAACACCTCC 
ACGGAAGTGA GCACAACCAG CACCGGTGCT GCCACTGGTG GTCCCCTCGA CTCCACCCTG 
ATGGGTGACG CCGCAAGTCA GAGCCCCCCA GAGAGTAGTG CTGCTCCTCC CCTGCCATCC 
CTGCGTCCCG TGACTGCCTT CACTCTCGAA GCAACAGTCG ACACACCAAC ACTGGCTACT 
GCCAAGCCGC CATATGTTTG TGATATCACA GTCCCCGATG CCTATCTGAT CACAACTGTG 
CTGGCCAGAA GAGCTGTGCA GGAGTACATC ATTACAGCAA TCAAAGAAGT ACTGAGGATT 
CACTTCAACC GTGCAGTGGA ACTGAAGGTT TACGAACTAT TTACTGACTT CACTTTTCTG 
GTAACATCCG GTCCTTTCGT TTACACGGCA ATATCCGTCA TAAATGTGCT TATAAACAGT 
AAGCTTGTCC GTGACCAGAC TCCTTTAATC CTGTCTGTGA AACCTTCTTT CCTTGTGCCA 
GAGTCCAGGT TCCAAGTTCA AACAGTACTT CAGTTTGTGC CTCCGAGTGT GGATACTGGC 
TTCTGCAACT TCACCCAGCG CATTGAGAAA GGCCTAATGA CAGCTCTCTT TGAAGTGAGA 
AAACACCACC AGGGAACGTA TAACCTCACG GTGCAGATCT TGAATATCAC CATCAGTTCC 
TCAAGGGTGA CTCCTCGGCG GGGCCCGGTG AATATCATCT TTGCGGTTAA AAGCACACAG 
GGATTTTTGA ATGGGTCGGA AGTGAGCGAG CTGCTCAGAA ACTTGAGTGT GGTGGAGTTC 
AGTTTCTATC TGGGATACCC AGTGCTGCAG ATCGCAGAGC CCTTCCAGTA TCCACAGCTC 
AACTTATCTC AGTTGCTGAA GTCCTCTTGG GTCAGAACAG TTCTCCTGGG CGTCATGGAG 
AAGCAACTCC AGAATGAAGT GTTTCAAGCC GAGATGGAAC GCAAGCTGGC CCAGCTGCTC 
AGCGAGGTTT CCACCAGAAG GCGGATGTGG AGAAGGGCCA CTGTAGCTGC AGGGAACAGT 
GTGGTGCAGG TGGTAAATGT GTCGAGGCTG GAGGGAGATG ACAATCCGGT ACAGCTCATC 
TACTTTGTGG AGGATCAAGA TGGAGAAAGA CTCAGTGCAG TCAAGTCTTC GGACCTGATT 
AACAAAATGG ACCTCCAGAG AGCAGCCATC ATCTTGGGTT ACCGAATTCA AGGTGTCATT 
GCCCAGCCTG TCGACAGGGT GAAGAGGCCG TCTCCGGAAT CCCAGAGCAA CAACTTGTGG 
GTCATTGTTG GCGTGGTCAT CCCAGTGCTG GTGGTGATGG TGATTGTTGT CATCCTCTAC 
TGGAAACTAT GCCGCACAGA CAAGCTAGAC TTTCAGCCTG ACACTGTGGC CAACATTCAG 
CAGCGTCAGA AGCTGCAGAT CCCTAGTGTG AAGGGCTTCG ATTTTGCTAA GCAGCATCTG 
GGTCAGCACA ATAAAGACGA CATATTGATT ATTCATGAGC CAGCGCCACT GCCAGGACCT 
CTGAAGGACC ACACCACGCC CTCGGAAAAT GGAGACGTGC CAAGCCCCAA GTCAAAGATC 
CCTTCCAAGA ATGTTCGTCA CAGAGGAAGA GTTTCTCCCT CAGATGCTGA CTCTACGGTC 
AGTGAAGAGT CCAGCGAGAG GGACGCAGGA GATAAGACGC CGGGAGCCGT CAACGATGGC 
AGGTCCCACA GAGCTCCGCA GAGCGGGCCA CCACTGCCCA GTTCGGGAAA TGAGCAGCAC 
TCATCAGCCT CCATCTTCGA GCACGTGGAC AGGATCTCCC GCCCCCCGGA GGCTAGCCGG 
CGGGTCCCCA GTAAGATCCA GCTTATCGCC ATGCAGCCGA TCCCGGCACC TCCCGTCCAG 
CGCCCCTCCC CAGCCGACCG AGTGGCGGAA AGCAATAAAA TCAACAAAGA GATTCAGACC 
GCGCTGCGGC ACAAGTCTGA GATCGAGCAC CATCGCAACA AGATCCGCCT GCGCGCCAAG 
CGCCGCGGGC ACTACGAGTT CCCGGTGGTA GACGACCTGT CCTCGGGCGA CACTAAGGAG 
CGACACCGGG TGTACCGCAG GGCACAGATG CAGATCGACA AGATCCTGGA CCCCACGGCC 
AGCGTGCCCT CCGTGTTCAT AGAGCCCAGG AAGAGCTCAC GGATAAAACG TTCTCCCAAG 
CCTCGCCGGA AACACCAGGT CAACGGCTGT CCTGCCGACG CTGAGAAGGA CCGGCTCATC 
ACCACAGACA GCGATGGCAC CTACAGGAGG CCCCCCGGCG TCCACAACTC AGCCTACATC 
GGATGCCCAT CGGATCCTGA CCTCCCAGCC GATGTGCAGA CACCATCCTC GGTGGAACTG 
GGGAGGTATC CAGCCCTTCC CTTCCCGGCC TCCCAGTACA TCCCACCCCA GCCGTCCATC 
GAGGAGGCAC GCCAGACCAT GCACTCCCTC CTGGACGACG CCTTTGCCCT CGTGGCCCCC 
AGCAGCCAGC CTGCCAGCAC CGCAGGTGTA GGCCCCGGAG TCCCACCCGG CCTGCCCGCA 
AACAGCACCC CTTCCCAGGA AGAGAGGCGA GCCACCCAGT GGGGGTCCTT CTACAGCCCA 
GCCCAGACGG CCAACAATCC CTGCAGTAGA TACGAAGACT ATGGAATGAC TCCCCCGACG 
GGTCCATTGC CAAGACCAGG TTTTGGCCCC GGTTTGCTGC AGTCTACAGA GCTGGTGCCC 
CCTGACCCTC AGCAGCCACA GGCCTCCGCC GAAGCCCCAT TTGCTGCCAG AGGGATCTAC 
TCGGAGGAGA TGCCGTCGGT GGCCCGGCCT CGGCCTGTCG GGGGTACCAC AGGCTCCCAG 
ATCCAGCACC TGACACAGGT GGGGATTGCC AGCAGAATTG GAGCTCAGCC AGTGGAAATC 
CCGCCAAGCA GAGGCAGCCA GTATGGGGGG CCAGGCTGGC CTTCGTACGG GGAGGACGAA 
GCGGGGCGAA GAGAGGCCGT GCCAAGGACT TCAGGCAGGG AGCCCTCAGC TCCTTCCGGG 
AACCTCCCCC ACCGGGGACT GCAGGGCCCT GGGCTGGGTT ACCCCACCAG CTCCACGGAA 
GACCTCCAGC CTGGCCACTC CTCGGCCTCT CTCATCAAAG CAATCCGCGA GGAGCTCCTC 
CGGCTCTCCC AGAAACAGAG CACCGTGCAG AACTTCCACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1549 ( NM_001164665.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158137.1 Homo sapiens KIAA1549 (KIAA1549), transcript variant 2, mRNA.

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CloneID	OHu22824
Clone ID Related Accession (Same CDS sequence)	NM_001164665.2 , NM_001164665.1
Accession Version	NM_001164665.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	UPF0606 protein KIAA1549 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC083868.3, AM989469.1 and AC018663.3. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001164665.1. Transcript Variant: This variant (2), also known as the long-form, v2, represents the longer transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGGTACGGT GTTGATCACT 
GACGCGTACC TGCCATCAGG ATCCTCGTTT GTTTCTGAAG CAACCCCCTT CCCTCTGCCC 
ACAGAGCTGA CCGTCGTGGG CCCATCACTC ACACCCACAG AGGTGCCACT GAACACCTCC 
ACGGAAGTGA GCACAACCAG CACCGGTGCT GCCACTGGTG GTCCCCTCGA CTCCACCCTG 
ATGGGTGACG CCGCAAGTCA GAGCCCCCCA GAGAGTAGTG CTGCTCCTCC CCTGCCATCC 
CTGCGTCCCG TGACTGCCTT CACTCTCGAA GCAACAGTCG ACACACCAAC ACTGGCTACT 
GCCAAGCCGC CATATGTTTG TGATATCACA GTCCCCGATG CCTATCTGAT CACAACTGTG 
CTGGCCAGAA GAGCTGTGCA GGAGTACATC ATTACAGCAA TCAAAGAAGT ACTGAGGATT 
CACTTCAACC GTGCAGTGGA ACTGAAGGTT TACGAACTAT TTACTGACTT CACTTTTCTG 
GTAACATCCG GTCCTTTCGT TTACACGGCA ATATCCGTCA TAAATGTGCT TATAAACAGT 
AAGCTTGTCC GTGACCAGAC TCCTTTAATC CTGTCTGTGA AACCTTCTTT CCTTGTGCCA 
GAGTCCAGGT TCCAAGTTCA AACAGTACTT CAGTTTGTGC CTCCGAGTGT GGATACTGGC 
TTCTGCAACT TCACCCAGCG CATTGAGAAA GGCCTAATGA CAGCTCTCTT TGAAGTGAGA 
AAACACCACC AGGGAACGTA TAACCTCACG GTGCAGATCT TGAATATCAC CATCAGTTCC 
TCAAGGGTGA CTCCTCGGCG GGGCCCGGTG AATATCATCT TTGCGGTTAA AAGCACACAG 
GGATTTTTGA ATGGGTCGGA AGTGAGCGAG CTGCTCAGAA ACTTGAGTGT GGTGGAGTTC 
AGTTTCTATC TGGGATACCC AGTGCTGCAG ATCGCAGAGC CCTTCCAGTA TCCACAGCTC 
AACTTATCTC AGTTGCTGAA GTCCTCTTGG GTCAGAACAG TTCTCCTGGG CGTCATGGAG 
AAGCAACTCC AGAATGAAGT GTTTCAAGCC GAGATGGAAC GCAAGCTGGC CCAGCTGCTC 
AGCGAGGTTT CCACCAGAAG GCGGATGTGG AGAAGGGCCA CTGTAGCTGC AGGGAACAGT 
GTGGTGCAGG TGGTAAATGT GTCGAGGCTG GAGGGAGATG ACAATCCGGT ACAGCTCATC 
TACTTTGTGG AGGATCAAGA TGGAGAAAGA CTCAGTGCAG TCAAGTCTTC GGACCTGATT 
AACAAAATGG ACCTCCAGAG AGCAGCCATC ATCTTGGGTT ACCGAATTCA AGGTGTCATT 
GCCCAGCCTG TCGACAGGGT GAAGAGGCCG TCTCCGGAAT CCCAGAGCAA CAACTTGTGG 
GTCATTGTTG GCGTGGTCAT CCCAGTGCTG GTGGTGATGG TGATTGTTGT CATCCTCTAC 
TGGAAACTAT GCCGCACAGA CAAGCTAGAC TTTCAGCCTG ACACTGTGGC CAACATTCAG 
CAGCGTCAGA AGCTGCAGAT CCCTAGTGTG AAGGGCTTCG ATTTTGCTAA GCAGCATCTG 
GGTCAGCACA ATAAAGACGA CATATTGATT ATTCATGAGC CAGCGCCACT GCCAGGACCT 
CTGAAGGACC ACACCACGCC CTCGGAAAAT GGAGACGTGC CAAGCCCCAA GTCAAAGATC 
CCTTCCAAGA ATGTTCGTCA CAGAGGAAGA GTTTCTCCCT CAGATGCTGA CTCTACGGTC 
AGTGAAGAGT CCAGCGAGAG GGACGCAGGA GATAAGACGC CGGGAGCCGT CAACGATGGC 
AGGTCCCACA GAGCTCCGCA GAGCGGGCCA CCACTGCCCA GTTCGGGAAA TGAGCAGCAC 
TCATCAGCCT CCATCTTCGA GCACGTGGAC AGGATCTCCC GCCCCCCGGA GGCTAGCCGG 
CGGGTCCCCA GTAAGATCCA GCTTATCGCC ATGCAGCCGA TCCCGGCACC TCCCGTCCAG 
CGCCCCTCCC CAGCCGACCG AGTGGCGGAA AGCAATAAAA TCAACAAAGA GATTCAGACC 
GCGCTGCGGC ACAAGTCTGA GATCGAGCAC CATCGCAACA AGATCCGCCT GCGCGCCAAG 
CGCCGCGGGC ACTACGAGTT CCCGGTGGTA GACGACCTGT CCTCGGGCGA CACTAAGGAG 
CGACACCGGG TGTACCGCAG GGCACAGATG CAGATCGACA AGATCCTGGA CCCCACGGCC 
AGCGTGCCCT CCGTGTTCAT AGAGCCCAGG AAGAGCTCAC GGATAAAACG TTCTCCCAAG 
CCTCGCCGGA AACACCAGGT CAACGGCTGT CCTGCCGACG CTGAGAAGGA CCGGCTCATC 
ACCACAGACA GCGATGGCAC CTACAGGAGG CCCCCCGGCG TCCACAACTC AGCCTACATC 
GGATGCCCAT CGGATCCTGA CCTCCCAGCC GATGTGCAGA CACCATCCTC GGTGGAACTG 
GGGAGGTATC CAGCCCTTCC CTTCCCGGCC TCCCAGTACA TCCCACCCCA GCCGTCCATC 
GAGGAGGCAC GCCAGACCAT GCACTCCCTC CTGGACGACG CCTTTGCCCT CGTGGCCCCC 
AGCAGCCAGC CTGCCAGCAC CGCAGGTGTA GGCCCCGGAG TCCCACCCGG CCTGCCCGCA 
AACAGCACCC CTTCCCAGGA AGAGAGGCGA GCCACCCAGT GGGGGTCCTT CTACAGCCCA 
GCCCAGACGG CCAACAATCC CTGCAGTAGA TACGAAGACT ATGGAATGAC TCCCCCGACG 
GGTCCATTGC CAAGACCAGG TTTTGGCCCC GGTTTGCTGC AGTCTACAGA GCTGGTGCCC 
CCTGACCCTC AGCAGCCACA GGCCTCCGCC GAAGCCCCAT TTGCTGCCAG AGGGATCTAC 
TCGGAGGAGA TGCCGTCGGT GGCCCGGCCT CGGCCTGTCG GGGGTACCAC AGGCTCCCAG 
ATCCAGCACC TGACACAGGT GGGGATTGCC AGCAGAATTG GAGCTCAGCC AGTGGAAATC 
CCGCCAAGCA GAGGCAGCCA GTATGGGGGG CCAGGCTGGC CTTCGTACGG GGAGGACGAA 
GCGGGGCGAA GAGAGGCCAC ACACATGCTC GGACATCAAG AGTATTCTTC TTCACCGCTA 
TTTCAGGTGC CAAGGACTTC AGGCAGGGAG CCCTCAGCTC CTTCCGGGAA CCTCCCCCAC 
CGGGGACTGC AGGGCCCTGG GCTGGGTTAC CCCACCAGCT CCACGGAAGA CCTCCAGCCT 
GGCCACTCCT CGGCCTCTCT CATCAAAGCA ATCCGCGAGG AGCTCCTCCG GCTCTCCCAG 
AAACAGAGCA CCGTGCAGAA CTTCCACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158137.1
CDS	121..5973
Translation	MPGARRRRRGAAMEGKPRAGVALAPGPSGRRPSARCARRRRPGLLLPGLWLLLLARPASCAPDELSPEQHNLSLYSMELVLKKSTGHSAAQVALTETAPGSQHSSPLHVTAPPSATTFDTAFFNQGKQTKSTADPSIFVATYVSVTSKEVAVNDDEMDNFLPDTHWTTPRMVSPIQYITVSPPGLPREALEPMLTPSLPMVSLQDEEVTSGWQNTTRQPAAYAESASHFHTFRSAFRTSEGIVPTPGRNLVLYPTDAYSHLSSRTLPEIVASLTEGVETTLFLSSRSLMPQPLGDGITIPLPSLGEVSQPPEEVWATSADRYTDVTTVLSQSLEETISPRTYPTVTASHAALAFSRTHSPLLSTPLAFASSASPTDVSSNPFLPSDSSKTSELHSNSALPGPVDNTHILSPVSSFRPYTWCAACTVPSPQQVLATSLMEKDVGSGDGAETLCMTVLEESSISLMSSVVADFSEFEEDPQVFNTLFPSRPIVPLSSRSMEISETSVGISAEVDMSSVTTTQVPPAHGRLSVPASLDPTAGSLSVAETQVTPSSVTTAFFSVITSILLDSSFSVIANKNTPSLAVRDPSVFTPYSLVPSVESSLFSDQERSSFSEHKPRGALDFASSFFSTPPLELSGSISSPSEAPASLSLMPSDLSPFTSQSFSPLVETFTLFDSSDLQSSQLSLPSSTNLEFSQLQPSSELPLNTIMLLPSRSEVSPWSSFPSDSLEFVEASTVSLTDSEAHFTSAFIETTSYLESSLISHESAVTALVPPGSESFDILTAGIQATSPLTTVHTTPILTESSLFSTLTPPDDQISALDGHVSVLASFSKAIPTGTVLITDAYLPSGSSFVSEATPFPLPTELTVVGPSLTPTEVPLNTSTEVSTTSTGAATGGPLDSTLMGDAASQSPPESSAAPPLPSLRPVTAFTLEATVDTPTLATAKPPYVCDITVPDAYLITTVLARRAVQEYIITAIKEVLRIHFNRAVELKVYELFTDFTFLVTSGPFVYTAISVINVLINSKLVRDQTPLILSVKPSFLVPESRFQVQTVLQFVPPSVDTGFCNFTQRIEKGLMTALFEVRKHHQGTYNLTVQILNITISSSRVTPRRGPVNIIFAVKSTQGFLNGSEVSELLRNLSVVEFSFYLGYPVLQIAEPFQYPQLNLSQLLKSSWVRTVLLGVMEKQLQNEVFQAEMERKLAQLLSEVSTRRRMWRRATVAAGNSVVQVVNVSRLEGDDNPVQLIYFVEDQDGERLSAVKSSDLINKMDLQRAAIILGYRIQGVIAQPVDRVKRPSPESQSNNLWVIVGVVIPVLVVMVIVVILYWKLCRTDKLDFQPDTVANIQQRQKLQIPSVKGFDFAKQHLGQHNKDDILIIHEPAPLPGPLKDHTTPSENGDVPSPKSKIPSKNVRHRGRVSPSDADSTVSEESSERDAGDKTPGAVNDGRSHRAPQSGPPLPSSGNEQHSSASIFEHVDRISRPPEASRRVPSKIQLIAMQPIPAPPVQRPSPADRVAESNKINKEIQTALRHKSEIEHHRNKIRLRAKRRGHYEFPVVDDLSSGDTKERHRVYRRAQMQIDKILDPTASVPSVFIEPRKSSRIKRSPKPRRKHQVNGCPADAEKDRLITTDSDGTYRRPPGVHNSAYIGCPSDPDLPADVQTPSSVELGRYPALPFPASQYIPPQPSIEEARQTMHSLLDDAFALVAPSSQPASTAGVGPGVPPGLPANSTPSQEERRATQWGSFYSPAQTANNPCSRYEDYGMTPPTGPLPRPGFGPGLLQSTELVPPDPQQPQASAEAPFAARGIYSEEMPSVARPRPVGGTTGSQIQHLTQVGIASRIGAQPVEIPPSRGSQYGGPGWPSYGEDEAGRREATHMLGHQEYSSSPLFQVPRTSGREPSAPSGNLPHRGLQGPGLGYPTSSTEDLQPGHSSASLIKAIREELLRLSQKQSTVQNFHS

Target ORF information:

RefSeq Version	NM_001164665.2
Organism	Homo sapiens(human)
Definition	Homo sapiens KIAA1549 (KIAA1549), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164665.2

ORF Insert Sequence:

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGGTACGGT GTTGATCACT 
GACGCGTACC TGCCATCAGG ATCCTCGTTT GTTTCTGAAG CAACCCCCTT CCCTCTGCCC 
ACAGAGCTGA CCGTCGTGGG CCCATCACTC ACACCCACAG AGGTGCCACT GAACACCTCC 
ACGGAAGTGA GCACAACCAG CACCGGTGCT GCCACTGGTG GTCCCCTCGA CTCCACCCTG 
ATGGGTGACG CCGCAAGTCA GAGCCCCCCA GAGAGTAGTG CTGCTCCTCC CCTGCCATCC 
CTGCGTCCCG TGACTGCCTT CACTCTCGAA GCAACAGTCG ACACACCAAC ACTGGCTACT 
GCCAAGCCGC CATATGTTTG TGATATCACA GTCCCCGATG CCTATCTGAT CACAACTGTG 
CTGGCCAGAA GAGCTGTGCA GGAGTACATC ATTACAGCAA TCAAAGAAGT ACTGAGGATT 
CACTTCAACC GTGCAGTGGA ACTGAAGGTT TACGAACTAT TTACTGACTT CACTTTTCTG 
GTAACATCCG GTCCTTTCGT TTACACGGCA ATATCCGTCA TAAATGTGCT TATAAACAGT 
AAGCTTGTCC GTGACCAGAC TCCTTTAATC CTGTCTGTGA AACCTTCTTT CCTTGTGCCA 
GAGTCCAGGT TCCAAGTTCA AACAGTACTT CAGTTTGTGC CTCCGAGTGT GGATACTGGC 
TTCTGCAACT TCACCCAGCG CATTGAGAAA GGCCTAATGA CAGCTCTCTT TGAAGTGAGA 
AAACACCACC AGGGAACGTA TAACCTCACG GTGCAGATCT TGAATATCAC CATCAGTTCC 
TCAAGGGTGA CTCCTCGGCG GGGCCCGGTG AATATCATCT TTGCGGTTAA AAGCACACAG 
GGATTTTTGA ATGGGTCGGA AGTGAGCGAG CTGCTCAGAA ACTTGAGTGT GGTGGAGTTC 
AGTTTCTATC TGGGATACCC AGTGCTGCAG ATCGCAGAGC CCTTCCAGTA TCCACAGCTC 
AACTTATCTC AGTTGCTGAA GTCCTCTTGG GTCAGAACAG TTCTCCTGGG CGTCATGGAG 
AAGCAACTCC AGAATGAAGT GTTTCAAGCC GAGATGGAAC GCAAGCTGGC CCAGCTGCTC 
AGCGAGGTTT CCACCAGAAG GCGGATGTGG AGAAGGGCCA CTGTAGCTGC AGGGAACAGT 
GTGGTGCAGG TGGTAAATGT GTCGAGGCTG GAGGGAGATG ACAATCCGGT ACAGCTCATC 
TACTTTGTGG AGGATCAAGA TGGAGAAAGA CTCAGTGCAG TCAAGTCTTC GGACCTGATT 
AACAAAATGG ACCTCCAGAG AGCAGCCATC ATCTTGGGTT ACCGAATTCA AGGTGTCATT 
GCCCAGCCTG TCGACAGGGT GAAGAGGCCG TCTCCGGAAT CCCAGAGCAA CAACTTGTGG 
GTCATTGTTG GCGTGGTCAT CCCAGTGCTG GTGGTGATGG TGATTGTTGT CATCCTCTAC 
TGGAAACTAT GCCGCACAGA CAAGCTAGAC TTTCAGCCTG ACACTGTGGC CAACATTCAG 
CAGCGTCAGA AGCTGCAGAT CCCTAGTGTG AAGGGCTTCG ATTTTGCTAA GCAGCATCTG 
GGTCAGCACA ATAAAGACGA CATATTGATT ATTCATGAGC CAGCGCCACT GCCAGGACCT 
CTGAAGGACC ACACCACGCC CTCGGAAAAT GGAGACGTGC CAAGCCCCAA GTCAAAGATC 
CCTTCCAAGA ATGTTCGTCA CAGAGGAAGA GTTTCTCCCT CAGATGCTGA CTCTACGGTC 
AGTGAAGAGT CCAGCGAGAG GGACGCAGGA GATAAGACGC CGGGAGCCGT CAACGATGGC 
AGGTCCCACA GAGCTCCGCA GAGCGGGCCA CCACTGCCCA GTTCGGGAAA TGAGCAGCAC 
TCATCAGCCT CCATCTTCGA GCACGTGGAC AGGATCTCCC GCCCCCCGGA GGCTAGCCGG 
CGGGTCCCCA GTAAGATCCA GCTTATCGCC ATGCAGCCGA TCCCGGCACC TCCCGTCCAG 
CGCCCCTCCC CAGCCGACCG AGTGGCGGAA AGCAATAAAA TCAACAAAGA GATTCAGACC 
GCGCTGCGGC ACAAGTCTGA GATCGAGCAC CATCGCAACA AGATCCGCCT GCGCGCCAAG 
CGCCGCGGGC ACTACGAGTT CCCGGTGGTA GACGACCTGT CCTCGGGCGA CACTAAGGAG 
CGACACCGGG TGTACCGCAG GGCACAGATG CAGATCGACA AGATCCTGGA CCCCACGGCC 
AGCGTGCCCT CCGTGTTCAT AGAGCCCAGG AAGAGCTCAC GGATAAAACG TTCTCCCAAG 
CCTCGCCGGA AACACCAGGT CAACGGCTGT CCTGCCGACG CTGAGAAGGA CCGGCTCATC 
ACCACAGACA GCGATGGCAC CTACAGGAGG CCCCCCGGCG TCCACAACTC AGCCTACATC 
GGATGCCCAT CGGATCCTGA CCTCCCAGCC GATGTGCAGA CACCATCCTC GGTGGAACTG 
GGGAGGTATC CAGCCCTTCC CTTCCCGGCC TCCCAGTACA TCCCACCCCA GCCGTCCATC 
GAGGAGGCAC GCCAGACCAT GCACTCCCTC CTGGACGACG CCTTTGCCCT CGTGGCCCCC 
AGCAGCCAGC CTGCCAGCAC CGCAGGTGTA GGCCCCGGAG TCCCACCCGG CCTGCCCGCA 
AACAGCACCC CTTCCCAGGA AGAGAGGCGA GCCACCCAGT GGGGGTCCTT CTACAGCCCA 
GCCCAGACGG CCAACAATCC CTGCAGTAGA TACGAAGACT ATGGAATGAC TCCCCCGACG 
GGTCCATTGC CAAGACCAGG TTTTGGCCCC GGTTTGCTGC AGTCTACAGA GCTGGTGCCC 
CCTGACCCTC AGCAGCCACA GGCCTCCGCC GAAGCCCCAT TTGCTGCCAG AGGGATCTAC 
TCGGAGGAGA TGCCGTCGGT GGCCCGGCCT CGGCCTGTCG GGGGTACCAC AGGCTCCCAG 
ATCCAGCACC TGACACAGGT GGGGATTGCC AGCAGAATTG GAGCTCAGCC AGTGGAAATC 
CCGCCAAGCA GAGGCAGCCA GTATGGGGGG CCAGGCTGGC CTTCGTACGG GGAGGACGAA 
GCGGGGCGAA GAGAGGCCAC ACACATGCTC GGACATCAAG AGTATTCTTC TTCACCGCTA 
TTTCAGGTGC CAAGGACTTC AGGCAGGGAG CCCTCAGCTC CTTCCGGGAA CCTCCCCCAC 
CGGGGACTGC AGGGCCCTGG GCTGGGTTAC CCCACCAGCT CCACGGAAGA CCTCCAGCCT 
GGCCACTCCT CGGCCTCTCT CATCAAAGCA ATCCGCGAGG AGCTCCTCCG GCTCTCCCAG 
AAACAGAGCA CCGTGCAGAA CTTCCACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1549 ( NM_001164665.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158137.1 Homo sapiens KIAA1549 (KIAA1549), transcript variant 2, mRNA.

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CloneID	OHu22824
Clone ID Related Accession (Same CDS sequence)	NM_001164665.2 , NM_001164665.1
Accession Version	NM_001164665.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	UPF0606 protein KIAA1549 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AM989469.1 and AC018663.3. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2), also known as the long-form, v2, represents the longer transcript and encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AM989469.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGGTACGGT GTTGATCACT 
GACGCGTACC TGCCATCAGG ATCCTCGTTT GTTTCTGAAG CAACCCCCTT CCCTCTGCCC 
ACAGAGCTGA CCGTCGTGGG CCCATCACTC ACACCCACAG AGGTGCCACT GAACACCTCC 
ACGGAAGTGA GCACAACCAG CACCGGTGCT GCCACTGGTG GTCCCCTCGA CTCCACCCTG 
ATGGGTGACG CCGCAAGTCA GAGCCCCCCA GAGAGTAGTG CTGCTCCTCC CCTGCCATCC 
CTGCGTCCCG TGACTGCCTT CACTCTCGAA GCAACAGTCG ACACACCAAC ACTGGCTACT 
GCCAAGCCGC CATATGTTTG TGATATCACA GTCCCCGATG CCTATCTGAT CACAACTGTG 
CTGGCCAGAA GAGCTGTGCA GGAGTACATC ATTACAGCAA TCAAAGAAGT ACTGAGGATT 
CACTTCAACC GTGCAGTGGA ACTGAAGGTT TACGAACTAT TTACTGACTT CACTTTTCTG 
GTAACATCCG GTCCTTTCGT TTACACGGCA ATATCCGTCA TAAATGTGCT TATAAACAGT 
AAGCTTGTCC GTGACCAGAC TCCTTTAATC CTGTCTGTGA AACCTTCTTT CCTTGTGCCA 
GAGTCCAGGT TCCAAGTTCA AACAGTACTT CAGTTTGTGC CTCCGAGTGT GGATACTGGC 
TTCTGCAACT TCACCCAGCG CATTGAGAAA GGCCTAATGA CAGCTCTCTT TGAAGTGAGA 
AAACACCACC AGGGAACGTA TAACCTCACG GTGCAGATCT TGAATATCAC CATCAGTTCC 
TCAAGGGTGA CTCCTCGGCG GGGCCCGGTG AATATCATCT TTGCGGTTAA AAGCACACAG 
GGATTTTTGA ATGGGTCGGA AGTGAGCGAG CTGCTCAGAA ACTTGAGTGT GGTGGAGTTC 
AGTTTCTATC TGGGATACCC AGTGCTGCAG ATCGCAGAGC CCTTCCAGTA TCCACAGCTC 
AACTTATCTC AGTTGCTGAA GTCCTCTTGG GTCAGAACAG TTCTCCTGGG CGTCATGGAG 
AAGCAACTCC AGAATGAAGT GTTTCAAGCC GAGATGGAAC GCAAGCTGGC CCAGCTGCTC 
AGCGAGGTTT CCACCAGAAG GCGGATGTGG AGAAGGGCCA CTGTAGCTGC AGGGAACAGT 
GTGGTGCAGG TGGTAAATGT GTCGAGGCTG GAGGGAGATG ACAATCCGGT ACAGCTCATC 
TACTTTGTGG AGGATCAAGA TGGAGAAAGA CTCAGTGCAG TCAAGTCTTC GGACCTGATT 
AACAAAATGG ACCTCCAGAG AGCAGCCATC ATCTTGGGTT ACCGAATTCA AGGTGTCATT 
GCCCAGCCTG TCGACAGGGT GAAGAGGCCG TCTCCGGAAT CCCAGAGCAA CAACTTGTGG 
GTCATTGTTG GCGTGGTCAT CCCAGTGCTG GTGGTGATGG TGATTGTTGT CATCCTCTAC 
TGGAAACTAT GCCGCACAGA CAAGCTAGAC TTTCAGCCTG ACACTGTGGC CAACATTCAG 
CAGCGTCAGA AGCTGCAGAT CCCTAGTGTG AAGGGCTTCG ATTTTGCTAA GCAGCATCTG 
GGTCAGCACA ATAAAGACGA CATATTGATT ATTCATGAGC CAGCGCCACT GCCAGGACCT 
CTGAAGGACC ACACCACGCC CTCGGAAAAT GGAGACGTGC CAAGCCCCAA GTCAAAGATC 
CCTTCCAAGA ATGTTCGTCA CAGAGGAAGA GTTTCTCCCT CAGATGCTGA CTCTACGGTC 
AGTGAAGAGT CCAGCGAGAG GGACGCAGGA GATAAGACGC CGGGAGCCGT CAACGATGGC 
AGGTCCCACA GAGCTCCGCA GAGCGGGCCA CCACTGCCCA GTTCGGGAAA TGAGCAGCAC 
TCATCAGCCT CCATCTTCGA GCACGTGGAC AGGATCTCCC GCCCCCCGGA GGCTAGCCGG 
CGGGTCCCCA GTAAGATCCA GCTTATCGCC ATGCAGCCGA TCCCGGCACC TCCCGTCCAG 
CGCCCCTCCC CAGCCGACCG AGTGGCGGAA AGCAATAAAA TCAACAAAGA GATTCAGACC 
GCGCTGCGGC ACAAGTCTGA GATCGAGCAC CATCGCAACA AGATCCGCCT GCGCGCCAAG 
CGCCGCGGGC ACTACGAGTT CCCGGTGGTA GACGACCTGT CCTCGGGCGA CACTAAGGAG 
CGACACCGGG TGTACCGCAG GGCACAGATG CAGATCGACA AGATCCTGGA CCCCACGGCC 
AGCGTGCCCT CCGTGTTCAT AGAGCCCAGG AAGAGCTCAC GGATAAAACG TTCTCCCAAG 
CCTCGCCGGA AACACCAGGT CAACGGCTGT CCTGCCGACG CTGAGAAGGA CCGGCTCATC 
ACCACAGACA GCGATGGCAC CTACAGGAGG CCCCCCGGCG TCCACAACTC AGCCTACATC 
GGATGCCCAT CGGATCCTGA CCTCCCAGCC GATGTGCAGA CACCATCCTC GGTGGAACTG 
GGGAGGTATC CAGCCCTTCC CTTCCCGGCC TCCCAGTACA TCCCACCCCA GCCGTCCATC 
GAGGAGGCAC GCCAGACCAT GCACTCCCTC CTGGACGACG CCTTTGCCCT CGTGGCCCCC 
AGCAGCCAGC CTGCCAGCAC CGCAGGTGTA GGCCCCGGAG TCCCACCCGG CCTGCCCGCA 
AACAGCACCC CTTCCCAGGA AGAGAGGCGA GCCACCCAGT GGGGGTCCTT CTACAGCCCA 
GCCCAGACGG CCAACAATCC CTGCAGTAGA TACGAAGACT ATGGAATGAC TCCCCCGACG 
GGTCCATTGC CAAGACCAGG TTTTGGCCCC GGTTTGCTGC AGTCTACAGA GCTGGTGCCC 
CCTGACCCTC AGCAGCCACA GGCCTCCGCC GAAGCCCCAT TTGCTGCCAG AGGGATCTAC 
TCGGAGGAGA TGCCGTCGGT GGCCCGGCCT CGGCCTGTCG GGGGTACCAC AGGCTCCCAG 
ATCCAGCACC TGACACAGGT GGGGATTGCC AGCAGAATTG GAGCTCAGCC AGTGGAAATC 
CCGCCAAGCA GAGGCAGCCA GTATGGGGGG CCAGGCTGGC CTTCGTACGG GGAGGACGAA 
GCGGGGCGAA GAGAGGCCAC ACACATGCTC GGACATCAAG AGTATTCTTC TTCACCGCTA 
TTTCAGGTGC CAAGGACTTC AGGCAGGGAG CCCTCAGCTC CTTCCGGGAA CCTCCCCCAC 
CGGGGACTGC AGGGCCCTGG GCTGGGTTAC CCCACCAGCT CCACGGAAGA CCTCCAGCCT 
GGCCACTCCT CGGCCTCTCT CATCAAAGCA ATCCGCGAGG AGCTCCTCCG GCTCTCCCAG 
AAACAGAGCA CCGTGCAGAA CTTCCACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158137.1
CDS	50..5902
Translation	MPGARRRRRGAAMEGKPRAGVALAPGPSGRRPSARCARRRRPGLLLPGLWLLLLARPASCAPDELSPEQHNLSLYSMELVLKKSTGHSAAQVALTETAPGSQHSSPLHVTAPPSATTFDTAFFNQGKQTKSTADPSIFVATYVSVTSKEVAVNDDEMDNFLPDTHWTTPRMVSPIQYITVSPPGLPREALEPMLTPSLPMVSLQDEEVTSGWQNTTRQPAAYAESASHFHTFRSAFRTSEGIVPTPGRNLVLYPTDAYSHLSSRTLPEIVASLTEGVETTLFLSSRSLMPQPLGDGITIPLPSLGEVSQPPEEVWATSADRYTDVTTVLSQSLEETISPRTYPTVTASHAALAFSRTHSPLLSTPLAFASSASPTDVSSNPFLPSDSSKTSELHSNSALPGPVDNTHILSPVSSFRPYTWCAACTVPSPQQVLATSLMEKDVGSGDGAETLCMTVLEESSISLMSSVVADFSEFEEDPQVFNTLFPSRPIVPLSSRSMEISETSVGISAEVDMSSVTTTQVPPAHGRLSVPASLDPTAGSLSVAETQVTPSSVTTAFFSVITSILLDSSFSVIANKNTPSLAVRDPSVFTPYSLVPSVESSLFSDQERSSFSEHKPRGALDFASSFFSTPPLELSGSISSPSEAPASLSLMPSDLSPFTSQSFSPLVETFTLFDSSDLQSSQLSLPSSTNLEFSQLQPSSELPLNTIMLLPSRSEVSPWSSFPSDSLEFVEASTVSLTDSEAHFTSAFIETTSYLESSLISHESAVTALVPPGSESFDILTAGIQATSPLTTVHTTPILTESSLFSTLTPPDDQISALDGHVSVLASFSKAIPTGTVLITDAYLPSGSSFVSEATPFPLPTELTVVGPSLTPTEVPLNTSTEVSTTSTGAATGGPLDSTLMGDAASQSPPESSAAPPLPSLRPVTAFTLEATVDTPTLATAKPPYVCDITVPDAYLITTVLARRAVQEYIITAIKEVLRIHFNRAVELKVYELFTDFTFLVTSGPFVYTAISVINVLINSKLVRDQTPLILSVKPSFLVPESRFQVQTVLQFVPPSVDTGFCNFTQRIEKGLMTALFEVRKHHQGTYNLTVQILNITISSSRVTPRRGPVNIIFAVKSTQGFLNGSEVSELLRNLSVVEFSFYLGYPVLQIAEPFQYPQLNLSQLLKSSWVRTVLLGVMEKQLQNEVFQAEMERKLAQLLSEVSTRRRMWRRATVAAGNSVVQVVNVSRLEGDDNPVQLIYFVEDQDGERLSAVKSSDLINKMDLQRAAIILGYRIQGVIAQPVDRVKRPSPESQSNNLWVIVGVVIPVLVVMVIVVILYWKLCRTDKLDFQPDTVANIQQRQKLQIPSVKGFDFAKQHLGQHNKDDILIIHEPAPLPGPLKDHTTPSENGDVPSPKSKIPSKNVRHRGRVSPSDADSTVSEESSERDAGDKTPGAVNDGRSHRAPQSGPPLPSSGNEQHSSASIFEHVDRISRPPEASRRVPSKIQLIAMQPIPAPPVQRPSPADRVAESNKINKEIQTALRHKSEIEHHRNKIRLRAKRRGHYEFPVVDDLSSGDTKERHRVYRRAQMQIDKILDPTASVPSVFIEPRKSSRIKRSPKPRRKHQVNGCPADAEKDRLITTDSDGTYRRPPGVHNSAYIGCPSDPDLPADVQTPSSVELGRYPALPFPASQYIPPQPSIEEARQTMHSLLDDAFALVAPSSQPASTAGVGPGVPPGLPANSTPSQEERRATQWGSFYSPAQTANNPCSRYEDYGMTPPTGPLPRPGFGPGLLQSTELVPPDPQQPQASAEAPFAARGIYSEEMPSVARPRPVGGTTGSQIQHLTQVGIASRIGAQPVEIPPSRGSQYGGPGWPSYGEDEAGRREATHMLGHQEYSSSPLFQVPRTSGREPSAPSGNLPHRGLQGPGLGYPTSSTEDLQPGHSSASLIKAIREELLRLSQKQSTVQNFHS

Target ORF information:

RefSeq Version	NM_001164665.1
Organism	Homo sapiens(human)
Definition	Homo sapiens KIAA1549 (KIAA1549), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164665.1

ORF Insert Sequence:

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGGTACGGT GTTGATCACT 
GACGCGTACC TGCCATCAGG ATCCTCGTTT GTTTCTGAAG CAACCCCCTT CCCTCTGCCC 
ACAGAGCTGA CCGTCGTGGG CCCATCACTC ACACCCACAG AGGTGCCACT GAACACCTCC 
ACGGAAGTGA GCACAACCAG CACCGGTGCT GCCACTGGTG GTCCCCTCGA CTCCACCCTG 
ATGGGTGACG CCGCAAGTCA GAGCCCCCCA GAGAGTAGTG CTGCTCCTCC CCTGCCATCC 
CTGCGTCCCG TGACTGCCTT CACTCTCGAA GCAACAGTCG ACACACCAAC ACTGGCTACT 
GCCAAGCCGC CATATGTTTG TGATATCACA GTCCCCGATG CCTATCTGAT CACAACTGTG 
CTGGCCAGAA GAGCTGTGCA GGAGTACATC ATTACAGCAA TCAAAGAAGT ACTGAGGATT 
CACTTCAACC GTGCAGTGGA ACTGAAGGTT TACGAACTAT TTACTGACTT CACTTTTCTG 
GTAACATCCG GTCCTTTCGT TTACACGGCA ATATCCGTCA TAAATGTGCT TATAAACAGT 
AAGCTTGTCC GTGACCAGAC TCCTTTAATC CTGTCTGTGA AACCTTCTTT CCTTGTGCCA 
GAGTCCAGGT TCCAAGTTCA AACAGTACTT CAGTTTGTGC CTCCGAGTGT GGATACTGGC 
TTCTGCAACT TCACCCAGCG CATTGAGAAA GGCCTAATGA CAGCTCTCTT TGAAGTGAGA 
AAACACCACC AGGGAACGTA TAACCTCACG GTGCAGATCT TGAATATCAC CATCAGTTCC 
TCAAGGGTGA CTCCTCGGCG GGGCCCGGTG AATATCATCT TTGCGGTTAA AAGCACACAG 
GGATTTTTGA ATGGGTCGGA AGTGAGCGAG CTGCTCAGAA ACTTGAGTGT GGTGGAGTTC 
AGTTTCTATC TGGGATACCC AGTGCTGCAG ATCGCAGAGC CCTTCCAGTA TCCACAGCTC 
AACTTATCTC AGTTGCTGAA GTCCTCTTGG GTCAGAACAG TTCTCCTGGG CGTCATGGAG 
AAGCAACTCC AGAATGAAGT GTTTCAAGCC GAGATGGAAC GCAAGCTGGC CCAGCTGCTC 
AGCGAGGTTT CCACCAGAAG GCGGATGTGG AGAAGGGCCA CTGTAGCTGC AGGGAACAGT 
GTGGTGCAGG TGGTAAATGT GTCGAGGCTG GAGGGAGATG ACAATCCGGT ACAGCTCATC 
TACTTTGTGG AGGATCAAGA TGGAGAAAGA CTCAGTGCAG TCAAGTCTTC GGACCTGATT 
AACAAAATGG ACCTCCAGAG AGCAGCCATC ATCTTGGGTT ACCGAATTCA AGGTGTCATT 
GCCCAGCCTG TCGACAGGGT GAAGAGGCCG TCTCCGGAAT CCCAGAGCAA CAACTTGTGG 
GTCATTGTTG GCGTGGTCAT CCCAGTGCTG GTGGTGATGG TGATTGTTGT CATCCTCTAC 
TGGAAACTAT GCCGCACAGA CAAGCTAGAC TTTCAGCCTG ACACTGTGGC CAACATTCAG 
CAGCGTCAGA AGCTGCAGAT CCCTAGTGTG AAGGGCTTCG ATTTTGCTAA GCAGCATCTG 
GGTCAGCACA ATAAAGACGA CATATTGATT ATTCATGAGC CAGCGCCACT GCCAGGACCT 
CTGAAGGACC ACACCACGCC CTCGGAAAAT GGAGACGTGC CAAGCCCCAA GTCAAAGATC 
CCTTCCAAGA ATGTTCGTCA CAGAGGAAGA GTTTCTCCCT CAGATGCTGA CTCTACGGTC 
AGTGAAGAGT CCAGCGAGAG GGACGCAGGA GATAAGACGC CGGGAGCCGT CAACGATGGC 
AGGTCCCACA GAGCTCCGCA GAGCGGGCCA CCACTGCCCA GTTCGGGAAA TGAGCAGCAC 
TCATCAGCCT CCATCTTCGA GCACGTGGAC AGGATCTCCC GCCCCCCGGA GGCTAGCCGG 
CGGGTCCCCA GTAAGATCCA GCTTATCGCC ATGCAGCCGA TCCCGGCACC TCCCGTCCAG 
CGCCCCTCCC CAGCCGACCG AGTGGCGGAA AGCAATAAAA TCAACAAAGA GATTCAGACC 
GCGCTGCGGC ACAAGTCTGA GATCGAGCAC CATCGCAACA AGATCCGCCT GCGCGCCAAG 
CGCCGCGGGC ACTACGAGTT CCCGGTGGTA GACGACCTGT CCTCGGGCGA CACTAAGGAG 
CGACACCGGG TGTACCGCAG GGCACAGATG CAGATCGACA AGATCCTGGA CCCCACGGCC 
AGCGTGCCCT CCGTGTTCAT AGAGCCCAGG AAGAGCTCAC GGATAAAACG TTCTCCCAAG 
CCTCGCCGGA AACACCAGGT CAACGGCTGT CCTGCCGACG CTGAGAAGGA CCGGCTCATC 
ACCACAGACA GCGATGGCAC CTACAGGAGG CCCCCCGGCG TCCACAACTC AGCCTACATC 
GGATGCCCAT CGGATCCTGA CCTCCCAGCC GATGTGCAGA CACCATCCTC GGTGGAACTG 
GGGAGGTATC CAGCCCTTCC CTTCCCGGCC TCCCAGTACA TCCCACCCCA GCCGTCCATC 
GAGGAGGCAC GCCAGACCAT GCACTCCCTC CTGGACGACG CCTTTGCCCT CGTGGCCCCC 
AGCAGCCAGC CTGCCAGCAC CGCAGGTGTA GGCCCCGGAG TCCCACCCGG CCTGCCCGCA 
AACAGCACCC CTTCCCAGGA AGAGAGGCGA GCCACCCAGT GGGGGTCCTT CTACAGCCCA 
GCCCAGACGG CCAACAATCC CTGCAGTAGA TACGAAGACT ATGGAATGAC TCCCCCGACG 
GGTCCATTGC CAAGACCAGG TTTTGGCCCC GGTTTGCTGC AGTCTACAGA GCTGGTGCCC 
CCTGACCCTC AGCAGCCACA GGCCTCCGCC GAAGCCCCAT TTGCTGCCAG AGGGATCTAC 
TCGGAGGAGA TGCCGTCGGT GGCCCGGCCT CGGCCTGTCG GGGGTACCAC AGGCTCCCAG 
ATCCAGCACC TGACACAGGT GGGGATTGCC AGCAGAATTG GAGCTCAGCC AGTGGAAATC 
CCGCCAAGCA GAGGCAGCCA GTATGGGGGG CCAGGCTGGC CTTCGTACGG GGAGGACGAA 
GCGGGGCGAA GAGAGGCCAC ACACATGCTC GGACATCAAG AGTATTCTTC TTCACCGCTA 
TTTCAGGTGC CAAGGACTTC AGGCAGGGAG CCCTCAGCTC CTTCCGGGAA CCTCCCCCAC 
CGGGGACTGC AGGGCCCTGG GCTGGGTTAC CCCACCAGCT CCACGGAAGA CCTCCAGCCT 
GGCCACTCCT CGGCCTCTCT CATCAAAGCA ATCCGCGAGG AGCTCCTCCG GCTCTCCCAG 
AAACAGAGCA CCGTGCAGAA CTTCCACAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1549 ( XM_011516442.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514744.1 Homo sapiens KIAA1549 (KIAA1549), transcript variant X1, mRNA.

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CloneID	OHu70837
Clone ID Related Accession (Same CDS sequence)	XM_011516442.2
Accession Version	XM_011516442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	UPF0606 protein KIAA1549 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGAGCTGAC CGTCGTGGGC 
CCATCACTCA CACCCACAGA GGTGCCACTG AACACCTCCA CGGAAGTGAG CACAACCAGC 
ACCGGTGCTG CCACTGGTGG TCCCCTCGAC TCCACCCTGA TGGGTGACGC CGCAAGTCAG 
AGCCCCCCAG AGAGTAGTGC TGCTCCTCCC CTGCCATCCC TGCGTCCCGT GACTGCCTTC 
ACTCTCGAAG CAACAGTCGA CACACCAACA CTGGCTACTG CCAAGCCGCC ATATGTTTGT 
GATATCACAG TCCCCGATGC CTATCTGATC ACAACTGTGC TGGCCAGAAG AGCTGTGCAG 
GAGTACATCA TTACAGCAAT CAAAGAAGTA CTGAGGATTC ACTTCAACCG TGCAGTGGAA 
CTGAAGGTTT ACGAACTATT TACTGACTTC ACTTTTCTGG TAACATCCGG TCCTTTCGTT 
TACACGGCAA TATCCGTCAT AAATGTGCTT ATAAACAGTA AGCTTGTCCG TGACCAGACT 
CCTTTAATCC TGTCTGTGAA ACCTTCTTTC CTTGTGCCAG AGTCCAGGTT CCAAGTTCAA 
ACAGTACTTC AGTTTGTGCC TCCGAGTGTG GATACTGGCT TCTGCAACTT CACCCAGCGC 
ATTGAGAAAG GCCTAATGAC AGCTCTCTTT GAAGTGAGAA AACACCACCA GGGAACGTAT 
AACCTCACGG TGCAGATCTT GAATATCACC ATCAGTTCCT CAAGGGTGAC TCCTCGGCGG 
GGCCCGGTGA ATATCATCTT TGCGGTTAAA AGCACACAGG GATTTTTGAA TGGGTCGGAA 
GTGAGCGAGC TGCTCAGAAA CTTGAGTGTG GTGGAGTTCA GTTTCTATCT GGGATACCCA 
GTGCTGCAGA TCGCAGAGCC CTTCCAGTAT CCACAGCTCA ACTTATCTCA GTTGCTGAAG 
TCCTCTTGGG TCAGAACAGT TCTCCTGGGC GTCATGGAGA AGCAACTCCA GAATGAAGTG 
TTTCAAGCCG AGATGGAACG CAAGCTGGCC CAGCTGCTCA GCGAGGTTTC CACCAGAAGG 
CGGATGTGGA GAAGGGCCAC TGTAGCTGCA GGGAACAGTG TGGTGCAGGT GGTAAATGTG 
TCGAGGCTGG AGGGAGATGA CAATCCGGTA CAGCTCATCT ACTTTGTGGA GGATCAAGAT 
GGAGAAAGAC TCAGTGCAGT CAAGTCTTCG GACCTGATTA ACAAAATGGA CCTCCAGAGA 
GCAGCCATCA TCTTGGGTTA CCGAATTCAA GGTGTCATTG CCCAGCCTGT CGACAGGGTG 
AAGAGGCCGT CTCCGGAATC CCAGAGCAAC AACTTGTGGG TCATTGTTGG CGTGGTCATC 
CCAGTGCTGG TGGTGATGGT GATTGTTGTC ATCCTCTACT GGAAACTATG CCGCACAGAC 
AAGCTAGACT TTCAGCCTGA CACTGTGGCC AACATTCAGC AGCGTCAGAA GCTGCAGATC 
CCTAGTGTGA AGGGCTTCGA TTTTGCTAAG CAGCATCTGG GTCAGCACAA TAAAGACGAC 
ATATTGATTA TTCATGAGCC AGCGCCACTG CCAGGACCTC TGAAGGACCA CACCACGCCC 
TCGGAAAATG GAGACGTGCC AAGCCCCAAG TCAAAGATCC CTTCCAAGAA TGTTCGTCAC 
AGAGGAAGAG TTTCTCCCTC AGATGCTGAC TCTACGGTCA GTGAAGAGTC CAGCGAGAGG 
GACGCAGGAG ATAAGACGCC GGGAGCCGTC AACGATGGCA GGTCCCACAG AGCTCCGCAG 
AGCGGGCCAC CACTGCCCAG TTCGGGAAAT GAGCAGCACT CATCAGCCTC CATCTTCGAG 
CACGTGGACA GGATCTCCCG CCCCCCGGAG GCTAGCCGGC GGGTCCCCAG TAAGATCCAG 
CTTATCGCCA TGCAGCCGAT CCCGGCACCT CCCGTCCAGC GCCCCTCCCC AGCCGACCGA 
GTGGCGGAAA GCAATAAAAT CAACAAAGAG ATTCAGACCG CGCTGCGGCA CAAGTCTGAG 
ATCGAGCACC ATCGCAACAA GATCCGCCTG CGCGCCAAGC GCCGCGGGCA CTACGAGTTC 
CCGGTGGTAG ACGACCTGTC CTCGGGCGAC ACTAAGGAGC GACACCGGGT GTACCGCAGG 
GCACAGATGC AGATCGACAA GATCCTGGAC CCCACGGCCA GCGTGCCCTC CGTGTTCATA 
GAGCCCAGGA AGAGCTCACG GATAAAACGT TCTCCCAAGC CTCGCCGGAA ACACCAGGTC 
AACGGCTGTC CTGCCGACGC TGAGAAGGAC CGGCTCATCA CCACAGACAG CGATGGCACC 
TACAGGAGGC CCCCCGGCGT CCACAACTCA GCCTACATCG GATGCCCATC GGATCCTGAC 
CTCCCAGCCG ATGTGCAGAC ACCATCCTCG GTGGAACTGG GGAGGTATCC AGCCCTTCCC 
TTCCCGGCCT CCCAGTACAT CCCACCCCAG CCGTCCATCG AGGAGGCACG CCAGACCATG 
CACTCCCTCC TGGACGACGC CTTTGCCCTC GTGGCCCCCA GCAGCCAGCC TGCCAGCACC 
GCAGGTGTAG GCCCCGGAGT CCCACCCGGC CTGCCCGCAA ACAGCACCCC TTCCCAGGAA 
GAGAGGCGAG CCACCCAGTG GGGGTCCTTC TACAGCCCAG CCCAGACGGC CAACAATCCC 
TGCAGTAGAT ACGAAGACTA TGGAATGACT CCCCCGACGG GTCCATTGCC AAGACCAGGT 
TTTGGCCCCG GTTTGCTGCA GTCTACAGAG CTGGTGCCCC CTGACCCTCA GCAGCCACAG 
GCCTCCGCCG AAGCCCCATT TGCTGCCAGA GGGATCTACT CGGAGGAGAT GCCGTCGGTG 
GCCCGGCCTC GGCCTGTCGG GGGTACCACA GGCTCCCAGA TCCAGCACCT GACACAGGTG 
GGGATTGCCA GCAGAATTGG AGCTCAGCCA GTGGAAATCC CGCCAAGCAG AGGCAGCCAG 
TATGGGGGGC CAGGCTGGCC TTCGTACGGG GAGGACGAAG CGGGGCGAAG AGAGGCCACA 
CACATGCTCG GACATCAAGA GTATTCTTCT TCACCGCTAT TTCAGGTGCC AAGGACTTCA 
GGCAGGGAGC CCTCAGCTCC TTCCGGGAAC CTCCCCCACC GGGGACTGCA GGGCCCTGGG 
CTGGGTTACC CCACCAGCTC CACGGAAGAC CTCCAGCCTG GCCACTCCTC GGCCTCTCTC 
ATCAAAGCAA TCCGCGAGGA GCTCCTCCGG CTCTCCCAGA AACAGAGCAC CGTGCAGAAC 
TTCCACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514744.1
CDS	358..6129
Translation	MPGARRRRRGAAMEGKPRAGVALAPGPSGRRPSARCARRRRPGLLLPGLWLLLLARPASCAPDELSPEQHNLSLYSMELVLKKSTGHSAAQVALTETAPGSQHSSPLHVTAPPSATTFDTAFFNQGKQTKSTADPSIFVATYVSVTSKEVAVNDDEMDNFLPDTHWTTPRMVSPIQYITVSPPGLPREALEPMLTPSLPMVSLQDEEVTSGWQNTTRQPAAYAESASHFHTFRSAFRTSEGIVPTPGRNLVLYPTDAYSHLSSRTLPEIVASLTEGVETTLFLSSRSLMPQPLGDGITIPLPSLGEVSQPPEEVWATSADRYTDVTTVLSQSLEETISPRTYPTVTASHAALAFSRTHSPLLSTPLAFASSASPTDVSSNPFLPSDSSKTSELHSNSALPGPVDNTHILSPVSSFRPYTWCAACTVPSPQQVLATSLMEKDVGSGDGAETLCMTVLEESSISLMSSVVADFSEFEEDPQVFNTLFPSRPIVPLSSRSMEISETSVGISAEVDMSSVTTTQVPPAHGRLSVPASLDPTAGSLSVAETQVTPSSVTTAFFSVITSILLDSSFSVIANKNTPSLAVRDPSVFTPYSLVPSVESSLFSDQERSSFSEHKPRGALDFASSFFSTPPLELSGSISSPSEAPASLSLMPSDLSPFTSQSFSPLVETFTLFDSSDLQSSQLSLPSSTNLEFSQLQPSSELPLNTIMLLPSRSEVSPWSSFPSDSLEFVEASTVSLTDSEAHFTSAFIETTSYLESSLISHESAVTALVPPGSESFDILTAGIQATSPLTTVHTTPILTESSLFSTLTPPDDQISALDGHVSVLASFSKAIPTELTVVGPSLTPTEVPLNTSTEVSTTSTGAATGGPLDSTLMGDAASQSPPESSAAPPLPSLRPVTAFTLEATVDTPTLATAKPPYVCDITVPDAYLITTVLARRAVQEYIITAIKEVLRIHFNRAVELKVYELFTDFTFLVTSGPFVYTAISVINVLINSKLVRDQTPLILSVKPSFLVPESRFQVQTVLQFVPPSVDTGFCNFTQRIEKGLMTALFEVRKHHQGTYNLTVQILNITISSSRVTPRRGPVNIIFAVKSTQGFLNGSEVSELLRNLSVVEFSFYLGYPVLQIAEPFQYPQLNLSQLLKSSWVRTVLLGVMEKQLQNEVFQAEMERKLAQLLSEVSTRRRMWRRATVAAGNSVVQVVNVSRLEGDDNPVQLIYFVEDQDGERLSAVKSSDLINKMDLQRAAIILGYRIQGVIAQPVDRVKRPSPESQSNNLWVIVGVVIPVLVVMVIVVILYWKLCRTDKLDFQPDTVANIQQRQKLQIPSVKGFDFAKQHLGQHNKDDILIIHEPAPLPGPLKDHTTPSENGDVPSPKSKIPSKNVRHRGRVSPSDADSTVSEESSERDAGDKTPGAVNDGRSHRAPQSGPPLPSSGNEQHSSASIFEHVDRISRPPEASRRVPSKIQLIAMQPIPAPPVQRPSPADRVAESNKINKEIQTALRHKSEIEHHRNKIRLRAKRRGHYEFPVVDDLSSGDTKERHRVYRRAQMQIDKILDPTASVPSVFIEPRKSSRIKRSPKPRRKHQVNGCPADAEKDRLITTDSDGTYRRPPGVHNSAYIGCPSDPDLPADVQTPSSVELGRYPALPFPASQYIPPQPSIEEARQTMHSLLDDAFALVAPSSQPASTAGVGPGVPPGLPANSTPSQEERRATQWGSFYSPAQTANNPCSRYEDYGMTPPTGPLPRPGFGPGLLQSTELVPPDPQQPQASAEAPFAARGIYSEEMPSVARPRPVGGTTGSQIQHLTQVGIASRIGAQPVEIPPSRGSQYGGPGWPSYGEDEAGRREATHMLGHQEYSSSPLFQVPRTSGREPSAPSGNLPHRGLQGPGLGYPTSSTEDLQPGHSSASLIKAIREELLRLSQKQSTVQNFHS

Target ORF information:

RefSeq Version	XM_011516442.2
Organism	Homo sapiens(human)
Definition	Homo sapiens KIAA1549 (KIAA1549), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516442.2

ORF Insert Sequence:

ATGCCGGGGG CGCGGCGCCG ACGCCGAGGC GCGGCCATGG AGGGGAAGCC CCGCGCCGGG 
GTCGCGCTGG CCCCGGGGCC GAGCGGCCGA CGGCCTTCCG CCCGCTGCGC CCGCCGCCGC 
CGCCCGGGGC TGCTGCTTCC TGGCCTCTGG CTGCTGCTGC TGGCCCGGCC GGCCTCGTGC 
GCCCCAGATG AGCTCTCTCC GGAACAGCAC AACCTTTCTT TATACTCCAT GGAGCTCGTG 
CTGAAGAAAA GCACTGGGCA CAGCGCTGCA CAAGTGGCCT TAACAGAAAC TGCTCCCGGC 
TCCCAGCACA GCAGTCCTCT CCATGTCACA GCCCCGCCGT CTGCCACTAC TTTTGATACA 
GCCTTTTTTA ACCAAGGAAA ACAGACCAAA AGTACAGCAG ATCCCAGCAT CTTTGTGGCA 
ACTTACGTGT CAGTGACGAG TAAAGAGGTG GCCGTCAATG ACGATGAGAT GGATAACTTT 
CTGCCAGATA CTCACTGGAC CACTCCACGG ATGGTTTCTC CAATACAGTA TATCACAGTC 
AGCCCACCAG GGCTGCCCAG GGAAGCATTA GAACCTATGC TCACTCCATC ATTACCCATG 
GTTTCTTTAC AAGATGAAGA AGTGACATCG GGCTGGCAGA ACACAACGCG ACAACCAGCG 
GCATATGCTG AGTCCGCCAG TCATTTCCAC ACCTTTCGGT CAGCTTTTCG CACCTCTGAG 
GGCATCGTTC CAACTCCTGG CAGGAATTTG GTGCTTTATC CTACTGATGC TTACAGTCAT 
TTATCAAGCA GGACTCTGCC AGAGATTGTG GCTTCCCTAA CAGAGGGTGT GGAAACCACC 
CTTTTTTTAA GCTCCCGGTC TTTAATGCCA CAGCCGTTAG GCGACGGCAT TACTATACCG 
TTGCCCTCCT TGGGGGAGGT CTCACAGCCT CCAGAGGAGG TTTGGGCCAC AAGTGCAGAC 
AGATACACTG ATGTGACCAC TGTGTTGAGT CAAAGCCTAG AAGAAACCAT CTCTCCAAGA 
ACATACCCCA CTGTGACTGC ATCGCACGCA GCCCTTGCAT TCAGCAGGAC ACATTCTCCA 
TTGCTTTCAA CTCCTCTTGC ATTTGCGTCC TCTGCTTCAC CAACTGATGT TTCATCTAAC 
CCCTTTCTCC CTAGCGACTC CAGCAAAACA TCCGAATTGC ATAGCAATTC AGCCCTCCCC 
GGTCCTGTGG ACAACACTCA TATCCTGAGC CCGGTGTCCT CATTCAGACC ATACACTTGG 
TGTGCGGCCT GCACTGTGCC TTCACCTCAG CAAGTTCTGG CCACGAGCCT CATGGAGAAA 
GACGTGGGAT CAGGGGATGG TGCCGAGACT CTGTGCATGA CCGTGCTGGA AGAAAGCAGC 
ATCTCTCTAA TGAGTAGCGT CGTAGCAGAC TTCTCTGAAT TTGAGGAAGA TCCTCAAGTA 
TTTAATACGC TTTTCCCCTC CAGACCTATC GTCCCACTTT CTTCTAGATC CATGGAAATC 
TCAGAGACGA GTGTTGGCAT TTCTGCCGAG GTGGATATGA GTAGTGTTAC AACCACACAG 
GTTCCCCCTG CCCACGGCCG CCTCTCTGTG CCGGCGTCAC TTGATCCTAC TGCTGGCTCC 
TTGTCTGTTG CTGAAACCCA AGTGACGCCA TCCAGCGTGA CCACTGCATT TTTCTCGGTC 
ATCACCAGCA TTCTCCTTGA CTCATCTTTC TCTGTCATAG CAAACAAAAA CACACCGTCG 
CTTGCCGTCA GAGACCCGAG TGTTTTTACG CCTTATAGTC TGGTTCCTTC AGTGGAGTCT 
TCACTTTTCT CTGACCAAGA ACGTTCCAGT TTTTCTGAGC ATAAACCCAG AGGTGCTTTG 
GATTTTGCAT CCAGCTTTTT CTCAACACCC CCGCTGGAAC TCAGCGGCTC CATCTCTTCG 
CCTTCGGAAG CACCTGCGTC TCTGTCTCTG ATGCCGAGTG ACTTGTCCCC CTTCACATCT 
CAGTCTTTTT CTCCCTTGGT TGAGACATTT ACATTGTTTG ACTCTAGTGA TCTGCAGTCA 
TCTCAGCTGT CTCTTCCCAG TTCCACAAAT CTTGAGTTTT CGCAGCTCCA GCCAAGTTCC 
GAGCTGCCTT TAAACACCAT CATGTTGCTA CCTAGCCGTT CTGAGGTGTC ACCATGGTCA 
AGCTTCCCTT CTGATTCTCT CGAGTTTGTT GAAGCGTCTA CGGTTTCACT GACGGATTCA 
GAAGCTCATT TTACCTCAGC TTTCATTGAA ACTACCTCCT ATCTTGAGTC TTCACTCATT 
TCCCATGAAT CCGCAGTCAC TGCACTGGTG CCCCCCGGCT CTGAGTCTTT TGACATTTTG 
ACTGCCGGGA TTCAAGCAAC ATCACCATTG ACCACTGTCC ACACAACGCC CATTTTAACT 
GAGTCTTCTT TGTTCTCAAC TCTGACACCT CCTGACGACC AAATCAGTGC TCTAGACGGT 
CACGTGTCTG TCCTGGCCTC TTTCTCCAAA GCCATTCCCA CTGAGCTGAC CGTCGTGGGC 
CCATCACTCA CACCCACAGA GGTGCCACTG AACACCTCCA CGGAAGTGAG CACAACCAGC 
ACCGGTGCTG CCACTGGTGG TCCCCTCGAC TCCACCCTGA TGGGTGACGC CGCAAGTCAG 
AGCCCCCCAG AGAGTAGTGC TGCTCCTCCC CTGCCATCCC TGCGTCCCGT GACTGCCTTC 
ACTCTCGAAG CAACAGTCGA CACACCAACA CTGGCTACTG CCAAGCCGCC ATATGTTTGT 
GATATCACAG TCCCCGATGC CTATCTGATC ACAACTGTGC TGGCCAGAAG AGCTGTGCAG 
GAGTACATCA TTACAGCAAT CAAAGAAGTA CTGAGGATTC ACTTCAACCG TGCAGTGGAA 
CTGAAGGTTT ACGAACTATT TACTGACTTC ACTTTTCTGG TAACATCCGG TCCTTTCGTT 
TACACGGCAA TATCCGTCAT AAATGTGCTT ATAAACAGTA AGCTTGTCCG TGACCAGACT 
CCTTTAATCC TGTCTGTGAA ACCTTCTTTC CTTGTGCCAG AGTCCAGGTT CCAAGTTCAA 
ACAGTACTTC AGTTTGTGCC TCCGAGTGTG GATACTGGCT TCTGCAACTT CACCCAGCGC 
ATTGAGAAAG GCCTAATGAC AGCTCTCTTT GAAGTGAGAA AACACCACCA GGGAACGTAT 
AACCTCACGG TGCAGATCTT GAATATCACC ATCAGTTCCT CAAGGGTGAC TCCTCGGCGG 
GGCCCGGTGA ATATCATCTT TGCGGTTAAA AGCACACAGG GATTTTTGAA TGGGTCGGAA 
GTGAGCGAGC TGCTCAGAAA CTTGAGTGTG GTGGAGTTCA GTTTCTATCT GGGATACCCA 
GTGCTGCAGA TCGCAGAGCC CTTCCAGTAT CCACAGCTCA ACTTATCTCA GTTGCTGAAG 
TCCTCTTGGG TCAGAACAGT TCTCCTGGGC GTCATGGAGA AGCAACTCCA GAATGAAGTG 
TTTCAAGCCG AGATGGAACG CAAGCTGGCC CAGCTGCTCA GCGAGGTTTC CACCAGAAGG 
CGGATGTGGA GAAGGGCCAC TGTAGCTGCA GGGAACAGTG TGGTGCAGGT GGTAAATGTG 
TCGAGGCTGG AGGGAGATGA CAATCCGGTA CAGCTCATCT ACTTTGTGGA GGATCAAGAT 
GGAGAAAGAC TCAGTGCAGT CAAGTCTTCG GACCTGATTA ACAAAATGGA CCTCCAGAGA 
GCAGCCATCA TCTTGGGTTA CCGAATTCAA GGTGTCATTG CCCAGCCTGT CGACAGGGTG 
AAGAGGCCGT CTCCGGAATC CCAGAGCAAC AACTTGTGGG TCATTGTTGG CGTGGTCATC 
CCAGTGCTGG TGGTGATGGT GATTGTTGTC ATCCTCTACT GGAAACTATG CCGCACAGAC 
AAGCTAGACT TTCAGCCTGA CACTGTGGCC AACATTCAGC AGCGTCAGAA GCTGCAGATC 
CCTAGTGTGA AGGGCTTCGA TTTTGCTAAG CAGCATCTGG GTCAGCACAA TAAAGACGAC 
ATATTGATTA TTCATGAGCC AGCGCCACTG CCAGGACCTC TGAAGGACCA CACCACGCCC 
TCGGAAAATG GAGACGTGCC AAGCCCCAAG TCAAAGATCC CTTCCAAGAA TGTTCGTCAC 
AGAGGAAGAG TTTCTCCCTC AGATGCTGAC TCTACGGTCA GTGAAGAGTC CAGCGAGAGG 
GACGCAGGAG ATAAGACGCC GGGAGCCGTC AACGATGGCA GGTCCCACAG AGCTCCGCAG 
AGCGGGCCAC CACTGCCCAG TTCGGGAAAT GAGCAGCACT CATCAGCCTC CATCTTCGAG 
CACGTGGACA GGATCTCCCG CCCCCCGGAG GCTAGCCGGC GGGTCCCCAG TAAGATCCAG 
CTTATCGCCA TGCAGCCGAT CCCGGCACCT CCCGTCCAGC GCCCCTCCCC AGCCGACCGA 
GTGGCGGAAA GCAATAAAAT CAACAAAGAG ATTCAGACCG CGCTGCGGCA CAAGTCTGAG 
ATCGAGCACC ATCGCAACAA GATCCGCCTG CGCGCCAAGC GCCGCGGGCA CTACGAGTTC 
CCGGTGGTAG ACGACCTGTC CTCGGGCGAC ACTAAGGAGC GACACCGGGT GTACCGCAGG 
GCACAGATGC AGATCGACAA GATCCTGGAC CCCACGGCCA GCGTGCCCTC CGTGTTCATA 
GAGCCCAGGA AGAGCTCACG GATAAAACGT TCTCCCAAGC CTCGCCGGAA ACACCAGGTC 
AACGGCTGTC CTGCCGACGC TGAGAAGGAC CGGCTCATCA CCACAGACAG CGATGGCACC 
TACAGGAGGC CCCCCGGCGT CCACAACTCA GCCTACATCG GATGCCCATC GGATCCTGAC 
CTCCCAGCCG ATGTGCAGAC ACCATCCTCG GTGGAACTGG GGAGGTATCC AGCCCTTCCC 
TTCCCGGCCT CCCAGTACAT CCCACCCCAG CCGTCCATCG AGGAGGCACG CCAGACCATG 
CACTCCCTCC TGGACGACGC CTTTGCCCTC GTGGCCCCCA GCAGCCAGCC TGCCAGCACC 
GCAGGTGTAG GCCCCGGAGT CCCACCCGGC CTGCCCGCAA ACAGCACCCC TTCCCAGGAA 
GAGAGGCGAG CCACCCAGTG GGGGTCCTTC TACAGCCCAG CCCAGACGGC CAACAATCCC 
TGCAGTAGAT ACGAAGACTA TGGAATGACT CCCCCGACGG GTCCATTGCC AAGACCAGGT 
TTTGGCCCCG GTTTGCTGCA GTCTACAGAG CTGGTGCCCC CTGACCCTCA GCAGCCACAG 
GCCTCCGCCG AAGCCCCATT TGCTGCCAGA GGGATCTACT CGGAGGAGAT GCCGTCGGTG 
GCCCGGCCTC GGCCTGTCGG GGGTACCACA GGCTCCCAGA TCCAGCACCT GACACAGGTG 
GGGATTGCCA GCAGAATTGG AGCTCAGCCA GTGGAAATCC CGCCAAGCAG AGGCAGCCAG 
TATGGGGGGC CAGGCTGGCC TTCGTACGGG GAGGACGAAG CGGGGCGAAG AGAGGCCACA 
CACATGCTCG GACATCAAGA GTATTCTTCT TCACCGCTAT TTCAGGTGCC AAGGACTTCA 
GGCAGGGAGC CCTCAGCTCC TTCCGGGAAC CTCCCCCACC GGGGACTGCA GGGCCCTGGG 
CTGGGTTACC CCACCAGCTC CACGGAAGAC CTCCAGCCTG GCCACTCCTC GGCCTCTCTC 
ATCAAAGCAA TCCGCGAGGA GCTCCTCCGG CTCTCCCAGA AACAGAGCAC CGTGCAGAAC 
TTCCACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Whole Chromosome 7 Gain Predicts Higher Risk of Recurrence in Pediatric Pilocytic Astrocytomas Independently From KIAA1549-BRAF Fusion Status.
Journal of neuropathology and experimental neurology75(4)306-15(2016 Apr)
Roth JJ,Fierst TM,Waanders AJ,Yimei L,Biegel JA,Santi M

Analysis of IDH1-R132 mutation, BRAF V600 mutation and KIAA1549-BRAF fusion transcript status in central nervous system tumors supports pediatric tumor classification.
Journal of cancer research and clinical oncology142(1)89-100(2016 Jan)
Gierke M,Sperveslage J,Schwab D,Beschorner R,Ebinger M,Schuhmann MU,Schittenhelm J

KIAA1549-BRAF fusions and IDH mutations can coexist in diffuse gliomas of adults.
Brain pathology (Zurich, Switzerland)22(6)841-7(2012 Nov)
Badiali M,Gleize V,Paris S,Moi L,Elhouadani S,Arcella A,Morace R,Antonelli M,Buttarelli FR,Figarella-Branger D,Kim YH,Ohgaki H,Mokhtari K,Sanson M,Giangaspero F

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KIAA1549 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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