ADGRB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADGRB2
Entrez Gene ID	576
Full Name	adhesion G protein-coupled receptor B2
Synonyms	BAI2
General protein information	Preferred Names adhesion G protein-coupled receptor B2 Names adhesion G protein-coupled receptor B2 brain-specific angiogenesis inhibitor 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p35.2
Summary	This gene encodes a a seven-span transmembrane protein that is thought to be a member of the secretin receptor family. The encoded protein is a brain-specific inhibitor of angiogenesis. The mature peptide may be further cleaved into additional products (PMID:20367554). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014].
Disorder MIM:	602683

mRNA and Protein(s)

mRNA	Protein	Name
NM_001364857.1	NP_001351786.1	adhesion G protein-coupled receptor B2 isoform 3 preproprotein
NM_001294335.2	NP_001281264.1	adhesion G protein-coupled receptor B2 isoform 1 preproprotein
XM_011541848.3	XP_011540150.1	adhesion G protein-coupled receptor B2 isoform X1
XM_011541849.2	XP_011540151.1	adhesion G protein-coupled receptor B2 isoform X3
XM_017001901.1	XP_016857390.1	adhesion G protein-coupled receptor B2 isoform X4
XM_017001909.1	XP_016857398.1	adhesion G protein-coupled receptor B2 isoform X12
XM_011541848.2	XP_011540150.1	adhesion G protein-coupled receptor B2 isoform X1
NM_001294336.2	NP_001281265.1	adhesion G protein-coupled receptor B2 isoform 2 preproprotein
XM_017001908.1	XP_016857397.1	adhesion G protein-coupled receptor B2 isoform X11
XM_017001910.1	XP_016857399.1	adhesion G protein-coupled receptor B2 isoform X13
XM_011541858.2	XP_011540160.1	adhesion G protein-coupled receptor B2 isoform X16
NM_001294335.1	NP_001281264.1	adhesion G protein-coupled receptor B2 isoform 1 preproprotein
XM_017001900.1	XP_016857389.1	adhesion G protein-coupled receptor B2 isoform X2
XM_017001903.1	XP_016857392.1	adhesion G protein-coupled receptor B2 isoform X6
XM_017001904.1	XP_016857393.1	adhesion G protein-coupled receptor B2 isoform X7
XM_017001905.1	XP_016857394.1	adhesion G protein-coupled receptor B2 isoform X8
XM_017001907.1	XP_016857396.1	adhesion G protein-coupled receptor B2 isoform X10
XM_017001911.1	XP_016857400.1	adhesion G protein-coupled receptor B2 isoform X14
NM_001294336.1	NP_001281265.1	adhesion G protein-coupled receptor B2 isoform 2 preproprotein
XM_017001902.1	XP_016857391.1	adhesion G protein-coupled receptor B2 isoform X5
XM_017001912.1	XP_016857401.1	adhesion G protein-coupled receptor B2 isoform X15
NM_001364857.2	NP_001351786.1	adhesion G protein-coupled receptor B2 isoform 3 preproprotein
XM_017001899.1	XP_016857388.1	adhesion G protein-coupled receptor B2 isoform X1
XM_024448673.1	XP_024304441.1	adhesion G protein-coupled receptor B2 isoform X3
XM_017001906.1	XP_016857395.1	adhesion G protein-coupled receptor B2 isoform X9

Pathways from BioSystems

Homologies

	BAI2	XP_001094702.2
Mus musculus (house mouse)	Bai2	NP_775094.2
Rattus norvegicus (Norway rat)	Bai2	NP_001101384.1
Homo sapiens (human)	BAI2	NP_001694.2
Pan troglodytes (chimpanzee)	BAI2	XP_003307988.1
Bos taurus (cattle)	BAI2	XP_003585838.2
Xenopus tropicalis (tropical clawed frog)	bai2	XP_002938534.2
Canis lupus familiaris (dog)	BAI2	XP_005617717.1
Gallus gallus (chicken)	BAI2	XP_423828.4

Gene Ontology
Bibliography

Related articles in PubMed

A disease-associated mutation in the adhesion GPCR BAI2 (ADGRB2) increases receptor signaling activity.
Purcell RH, Toro C, Gahl WA, Hall RA
Human mutation38(12)1751-1760(2017 Dec)

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Hamann J, Aust G, Ara? D, Engel FB, Formstone C, Fredriksson R, Hall RA, Harty BL, Kirchhoff C, Knapp B, Krishnan A, Liebscher I, Lin HH, Martinelli DC, Monk KR, Peeters MC, Piao X, Pr?mel S, Sch?neberg T, Schwartz TW, Singer K, Stacey M, Ushkaryov YA, Vallon M, Wolfrum U, Wright MW, Xu L, Langenhan T, Schi?th HB
Pharmacological reviews67(2)338-67(2015)

Identification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting protein.
Zencir S, Ovee M, Dobson MJ, Banerjee M, Topcu Z, Mohanty S
Biochemical and biophysical research communications411(4)792-7(2011 Aug)

Brain-specific angiogenesis inhibitor 2 (BAI2) may be activated by proteolytic processing.
Okajima D, Kudo G, Yokota H
Journal of receptor and signal transduction research30(3)143-53(2010 Jun)

Brain-specific angiogenesis inhibitor 2 regulates VEGF through GABP that acts as a transcriptional repressor.
Jeong BC, Kim MY, Lee JH, Kee HJ, Kho DH, Han KE, Qian YR, Kim JK, Kim KK
FEBS letters580(2)669-76(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first study reporting BAI2 as an interaction partner of GIP.
Title: Identification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting protein.

Observational study of gene-disease association. (HuGE Navigator)
Title: An approach based on a genome-wide association study reveals candidate loci for narcolepsy.

BAI2 is a functional GPCR regulated by proteolytic processing and activates the NFAT pathway
Title: Brain-specific angiogenesis inhibitor 2 (BAI2) may be activated by proteolytic processing.

involvement of BAI2 in the ischemia-induced brain angiogenesis
Title: Expression of brain-specific angiogenesis inhibitor 2 (BAI2) in normal and ischemic brain: involvement of BAI2 in the ischemia-induced brain angiogenesis.

The following ADGRB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu56451	XM_011541858.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu56448	NM_001364857.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu90315	XM_017001902.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu90316	XM_017001903.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu90317	XM_017001906.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu28697	NM_001294335.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28698	NM_001294336.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28697	XM_017001900.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28698	XM_017001905.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu33641	XM_017001901.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu33642	XM_017001904.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu33643	XM_017001907.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu33644	XM_017001908.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu33645	XM_017001909.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu33646	XM_017001910.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu33647	XM_017001911.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu56448	XM_011541848.3 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu56448	XM_017001899.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu56449	XM_011541849.2 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu56449	XM_024448673.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu56450	XM_017001912.1 Latest version!	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu56448	XM_011541848.2	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28697	NM_001294335.1	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28698	NM_001294336.1	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu56448	NM_001364857.1	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADGRB2 ( XM_011541858.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540160.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OHu56451
Clone ID Related Accession (Same CDS sequence)	XM_011541858.2
Accession Version	XM_011541858.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGA CAGGCGACCC GGCGGCTGAG GAGTGGTCCC CGTGGAGCGT GTGTTCCCTG 
ACGTGTGGGC AGGGTCTGCA GGTGCGGACC CGCTCCTGTG TGTCCTCCCC CTATGGGACC 
CTGTGCAGCG GGCCCCTGCG GGAGACCAGG CCCTGCAACA ATTCAGCCAC CTGCCCAGTG 
CACGGCGTGT GGGAGGAGTG GGGGTCCTGG AGCCTGTGCT CCCGCAGCTG CGGGCGGGGG 
TCCCGGAGCC GGATGCGGAC CTGCGTGCCC CCCCAGCACG GCGGCAAGGC CTGCGAGGGT 
CCTGAGCTGC AGACTAAGCT CTGCAGTATG GCTGCCTGCC CGGTGGAAGG CCAGTGGTTA 
GAATGGGGTC CCTGGGGCCC ATGCTCCACG TCCTGTGCCA ATGGGACCCA ACAGCGCAGC 
CGGAAGTGCA GCGTGGCGGG CCCAGCCTGG GCCACATGCA CGGGTGCCCT CACTGACACC 
CGGGAGTGCA GCAACCTCGA GTGCCCGGCC ACTGATAGCA AGTGGGGGCC ATGGAATGCG 
TGGAGCCTGT GCTCTAAGAC GTGTGACACA GGCTGGCAGC GCCGCTTCCG CATGTGCCAG 
GCCACGGGCA CGCAGGGCTA CCCCTGCGAG GGCACCGGAG AGGAGGTGAA GCCTTGTAGT 
GAGAAGAGGT GTCCAGCCTT CCATGAGATG TGCAGGGATG AGTACGTGAT GCTGATGACG 
TGGAAGAAGG CAGCTGCTGG CGAGATCATC TACAACAAGT GCCCCCCGAA TGCCTCAGGG 
TCTGCCAGCC GCCGCTGTCT CCTCAGTGCC CAAGGCGTGG CGTACTGGGG GCTGCCCAGC 
TTTGCTCGCT GCATCTCCCA TGAGTACCGC TACCTGTATC TGTCACTTAG GGAGCACCTG 
GCCAAGGGGC AGCGCATGCT GGCAGGCGAG GGCATGTCGC AGGTGGTGCG CAGCCTGCAG 
GAGCTACTGG CCCGGCGCAC CTACTATAGT GGGGACCTGC TCTTCTCTGT GGACATTCTG 
AGGAATGTCA CTGACACCTT TAAGAGGGCC ACCTACGTGC CCTCGGCTGA TGATGTGCAG 
CGCTTCTTCC AGGTGGTGAG CTTCATGGTG GATGCGGAAA ACAAGGAGAA GTGGGACGAT 
GCTCAGCAGG TGTCCCCTGG CTCTGTGCAC CTGCTCCGTG TCGTGGAGGA CTTCATTCAC 
CTGGTGGGCG ATGCTCTCAA GGCCTTCCAG AGCTCTCTGA TTGTCACAGA TAATCTAGTG 
ATCAGCATTC AGCGAGAGCC CGTCTCAGCT GTGTCCAGTG ACATCACGTT CCCCATGCGG 
GGCCGCCGGG GCATGAAGGA CTGGGTGCGG CACTCAGAGG ACCGCCTCTT CCTGCCCAAG 
GAGGTGCTCA GCCTCTCCTC CCCAGGGAAG CCAGCCACAT CTGGGGCAGC AGGCAGCCCT 
GGCAGGGGGA GGGGCCCAGG AACGGTGCCT CCTGGCCCAG GCCACTCCCA CCAGCGCCTC 
CTCCCAGCAG ACCCTGATGA GTCCTCCTAC TTTGTGATCG GTGCTGTACT CTACCGCACC 
CTTGGCCTCA TCCTGCCGCC TCCCAGGCCC CCGCTGGCCG TCACATCCCG GGTGATGACA 
GTGACTGTGC GCCCCCCTAC CCAGCCTCCA GCTGAGCCCC TCATCACTGT GGAGCTCTCC 
TACATCATCA ATGGGACCAC GGATCCCCAT TGCGCCAGCT GGGACTACTC CAGAGCAGAT 
GCCAGCTCAG GAGACTGGGA CACTGAAAAT TGCCAGACCC TGGAGACCCA GGCAGCTCAC 
ACCCGCTGCC AGTGCCAGCA CCTGTCCACC TTTGCTGTAC TAGCCCAGCC GCCCAAGGAC 
CTGACCCTGG AGCTGGCGGG CTCCCCCTCG GTCCCCCTGG TGATCGGCTG TGCAGTGTCG 
TGCATGGCGC TGCTCACCCT GCTCGCCATC TATGCCGCCT TTTGGAGGTT CATAAAATCT 
GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCT TGGCATCCAA CATCCTGATC 
CTCGTGGGCC AGTCCCGGGT GCTGAGCAAG GGCGTGTGCA CCATGACGGC TGCCTTCCTG 
CACTTCTTCT TTCTCTCCTC CTTTTGCTGG GTGCTTACCG AGGCCTGGCA GTCCTACCTG 
GCTGTCATTG GGCGGATGCG CACCCGCCTC GTTCGCAAGC GCTTCCTCTG CCTGGGCTGG 
GGTCTGCCTG CCCTGGTGGT GGCCGTGTCT GTTGGCTTTA CCCGAACGAA AGGATACGGT 
ACATCCAGCT ACTGCTGGCT CTCCCTGGAG GGCGGCCTGC TCTACGCCTT TGTGGGCCCT 
GCAGCCGTCA TTGTCCTGGT GAACATGCTC ATCGGAATCA TCGTCTTCAA CAAGCTCATG 
GCACGTGATG GCATCTCCGA CAAATCCAAG AAGCAGAGGG CCGGGTCGGA GCGGTGCCCC 
TGGGCCAGCC TGCTCCTCCC CTGCTCAGCG TGTGGAGCGG TCCCCAGCCC CCTGCTCAGC 
TCAGCCTCGG CCAGGAACGC CATGGCCTCA CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG 
CTGGCGCTCA CCTGGATGTC TGCCGTCCTG GCTATGACAG ACCGCCGTTC CGTCCTCTTC 
CAGGCCCTCT TTGCTGTCTT CAACTCCGCG CAGGGCTTTG TCATCACTGC TGTGCACTGC 
TTCCTGCGCC GAGAGGTCCA GGATGTGGTG AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT 
GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG AACGGGCAGC TGCAGATCCT GTCAGACTTT 
GAAAAGGATG TGGATCTGGC TTGTCAAACA GTGCTGTTCA AGGAGGTCAA CACTTGCAAC 
CCGTCCACCA TCACGGGCAC ACTATCCCGC CTGTCCCTGG ATGAGGATGA GGAGCCCAAG 
TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC AGCTTCTCAC CACTGCCTGG GAATATCCTG 
GTGCCCATGG CAGCCTCACC AGGGCTGGGG GAGCCTCCGC CCCCACAGGA GGCCAACCCT 
GTTTACATGT GTGGGGAGGG TGGCCTGCGG CAGCTGGACC TCACATGGCT GCGGCCCACT 
GAGCCAGGCT CTGAGGGAGA CTACATGGTG CTGCCCCGGC GGACTTTGAG CCTGCAGCCT 
GGCGGTGGGG GTGGAGGTGG TGAGGATGCC CCCAGGGCCC GGCCGGAGGG GACCCCCCGG 
CGAGCTGCCA AGACAGTGGC CCACACTGAA GGCTACCCCA GCTTCCTGTC CGTGGACCAC 
TCGGGCCTGG GGCTGGGCCC TGCCTATGGA TCTCTCCAGA ATCCCTATGG AATGACCTTC 
CAACCGCCAC CGCCGACACC CAGCGCCCGC CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG 
ACCATGCCTC GCACCGTGCC CGGCTCTACC ATGAAGATGG GCTCCCTGGA GCGAAAGAAA 
TTACGGTATT CAGACCTGGA CTTTGAGAAG GTGATGCACA CCCGGAAACG GCATTCAGAA 
CTCTACCACG AGCTCAACCA GAAGTTCCAC ACTTTCGACC GCTACCGCAG CCAGTCCACG 
GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC TCGGGTGGGG CAGCCGAGCG GAGCGTGTGC 
ACCGATAAGC CCAGCCCTGG GGAGCGCCCC AGCTTGTCCC AACATCGGCG CCATCAGAGC 
TGGAGCACCT TCAAATCTAT GACACTGGGC TCGCTGCCCC CCAAGCCCCG AGAACGGCTG 
ACTCTGCACC GGGCAGCAGC CTGGGAGCCC ACAGAACCAC CGGATGGTGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540160.1
CDS	233..4081
Translation	MAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011541858.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541858.2

ORF Insert Sequence:

ATGGCGCAGA CAGGCGACCC GGCGGCTGAG GAGTGGTCCC CGTGGAGCGT GTGTTCCCTG 
ACGTGTGGGC AGGGTCTGCA GGTGCGGACC CGCTCCTGTG TGTCCTCCCC CTATGGGACC 
CTGTGCAGCG GGCCCCTGCG GGAGACCAGG CCCTGCAACA ATTCAGCCAC CTGCCCAGTG 
CACGGCGTGT GGGAGGAGTG GGGGTCCTGG AGCCTGTGCT CCCGCAGCTG CGGGCGGGGG 
TCCCGGAGCC GGATGCGGAC CTGCGTGCCC CCCCAGCACG GCGGCAAGGC CTGCGAGGGT 
CCTGAGCTGC AGACTAAGCT CTGCAGTATG GCTGCCTGCC CGGTGGAAGG CCAGTGGTTA 
GAATGGGGTC CCTGGGGCCC ATGCTCCACG TCCTGTGCCA ATGGGACCCA ACAGCGCAGC 
CGGAAGTGCA GCGTGGCGGG CCCAGCCTGG GCCACATGCA CGGGTGCCCT CACTGACACC 
CGGGAGTGCA GCAACCTCGA GTGCCCGGCC ACTGATAGCA AGTGGGGGCC ATGGAATGCG 
TGGAGCCTGT GCTCTAAGAC GTGTGACACA GGCTGGCAGC GCCGCTTCCG CATGTGCCAG 
GCCACGGGCA CGCAGGGCTA CCCCTGCGAG GGCACCGGAG AGGAGGTGAA GCCTTGTAGT 
GAGAAGAGGT GTCCAGCCTT CCATGAGATG TGCAGGGATG AGTACGTGAT GCTGATGACG 
TGGAAGAAGG CAGCTGCTGG CGAGATCATC TACAACAAGT GCCCCCCGAA TGCCTCAGGG 
TCTGCCAGCC GCCGCTGTCT CCTCAGTGCC CAAGGCGTGG CGTACTGGGG GCTGCCCAGC 
TTTGCTCGCT GCATCTCCCA TGAGTACCGC TACCTGTATC TGTCACTTAG GGAGCACCTG 
GCCAAGGGGC AGCGCATGCT GGCAGGCGAG GGCATGTCGC AGGTGGTGCG CAGCCTGCAG 
GAGCTACTGG CCCGGCGCAC CTACTATAGT GGGGACCTGC TCTTCTCTGT GGACATTCTG 
AGGAATGTCA CTGACACCTT TAAGAGGGCC ACCTACGTGC CCTCGGCTGA TGATGTGCAG 
CGCTTCTTCC AGGTGGTGAG CTTCATGGTG GATGCGGAAA ACAAGGAGAA GTGGGACGAT 
GCTCAGCAGG TGTCCCCTGG CTCTGTGCAC CTGCTCCGTG TCGTGGAGGA CTTCATTCAC 
CTGGTGGGCG ATGCTCTCAA GGCCTTCCAG AGCTCTCTGA TTGTCACAGA TAATCTAGTG 
ATCAGCATTC AGCGAGAGCC CGTCTCAGCT GTGTCCAGTG ACATCACGTT CCCCATGCGG 
GGCCGCCGGG GCATGAAGGA CTGGGTGCGG CACTCAGAGG ACCGCCTCTT CCTGCCCAAG 
GAGGTGCTCA GCCTCTCCTC CCCAGGGAAG CCAGCCACAT CTGGGGCAGC AGGCAGCCCT 
GGCAGGGGGA GGGGCCCAGG AACGGTGCCT CCTGGCCCAG GCCACTCCCA CCAGCGCCTC 
CTCCCAGCAG ACCCTGATGA GTCCTCCTAC TTTGTGATCG GTGCTGTACT CTACCGCACC 
CTTGGCCTCA TCCTGCCGCC TCCCAGGCCC CCGCTGGCCG TCACATCCCG GGTGATGACA 
GTGACTGTGC GCCCCCCTAC CCAGCCTCCA GCTGAGCCCC TCATCACTGT GGAGCTCTCC 
TACATCATCA ATGGGACCAC GGATCCCCAT TGCGCCAGCT GGGACTACTC CAGAGCAGAT 
GCCAGCTCAG GAGACTGGGA CACTGAAAAT TGCCAGACCC TGGAGACCCA GGCAGCTCAC 
ACCCGCTGCC AGTGCCAGCA CCTGTCCACC TTTGCTGTAC TAGCCCAGCC GCCCAAGGAC 
CTGACCCTGG AGCTGGCGGG CTCCCCCTCG GTCCCCCTGG TGATCGGCTG TGCAGTGTCG 
TGCATGGCGC TGCTCACCCT GCTCGCCATC TATGCCGCCT TTTGGAGGTT CATAAAATCT 
GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCT TGGCATCCAA CATCCTGATC 
CTCGTGGGCC AGTCCCGGGT GCTGAGCAAG GGCGTGTGCA CCATGACGGC TGCCTTCCTG 
CACTTCTTCT TTCTCTCCTC CTTTTGCTGG GTGCTTACCG AGGCCTGGCA GTCCTACCTG 
GCTGTCATTG GGCGGATGCG CACCCGCCTC GTTCGCAAGC GCTTCCTCTG CCTGGGCTGG 
GGTCTGCCTG CCCTGGTGGT GGCCGTGTCT GTTGGCTTTA CCCGAACGAA AGGATACGGT 
ACATCCAGCT ACTGCTGGCT CTCCCTGGAG GGCGGCCTGC TCTACGCCTT TGTGGGCCCT 
GCAGCCGTCA TTGTCCTGGT GAACATGCTC ATCGGAATCA TCGTCTTCAA CAAGCTCATG 
GCACGTGATG GCATCTCCGA CAAATCCAAG AAGCAGAGGG CCGGGTCGGA GCGGTGCCCC 
TGGGCCAGCC TGCTCCTCCC CTGCTCAGCG TGTGGAGCGG TCCCCAGCCC CCTGCTCAGC 
TCAGCCTCGG CCAGGAACGC CATGGCCTCA CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG 
CTGGCGCTCA CCTGGATGTC TGCCGTCCTG GCTATGACAG ACCGCCGTTC CGTCCTCTTC 
CAGGCCCTCT TTGCTGTCTT CAACTCCGCG CAGGGCTTTG TCATCACTGC TGTGCACTGC 
TTCCTGCGCC GAGAGGTCCA GGATGTGGTG AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT 
GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG AACGGGCAGC TGCAGATCCT GTCAGACTTT 
GAAAAGGATG TGGATCTGGC TTGTCAAACA GTGCTGTTCA AGGAGGTCAA CACTTGCAAC 
CCGTCCACCA TCACGGGCAC ACTATCCCGC CTGTCCCTGG ATGAGGATGA GGAGCCCAAG 
TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC AGCTTCTCAC CACTGCCTGG GAATATCCTG 
GTGCCCATGG CAGCCTCACC AGGGCTGGGG GAGCCTCCGC CCCCACAGGA GGCCAACCCT 
GTTTACATGT GTGGGGAGGG TGGCCTGCGG CAGCTGGACC TCACATGGCT GCGGCCCACT 
GAGCCAGGCT CTGAGGGAGA CTACATGGTG CTGCCCCGGC GGACTTTGAG CCTGCAGCCT 
GGCGGTGGGG GTGGAGGTGG TGAGGATGCC CCCAGGGCCC GGCCGGAGGG GACCCCCCGG 
CGAGCTGCCA AGACAGTGGC CCACACTGAA GGCTACCCCA GCTTCCTGTC CGTGGACCAC 
TCGGGCCTGG GGCTGGGCCC TGCCTATGGA TCTCTCCAGA ATCCCTATGG AATGACCTTC 
CAACCGCCAC CGCCGACACC CAGCGCCCGC CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG 
ACCATGCCTC GCACCGTGCC CGGCTCTACC ATGAAGATGG GCTCCCTGGA GCGAAAGAAA 
TTACGGTATT CAGACCTGGA CTTTGAGAAG GTGATGCACA CCCGGAAACG GCATTCAGAA 
CTCTACCACG AGCTCAACCA GAAGTTCCAC ACTTTCGACC GCTACCGCAG CCAGTCCACG 
GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC TCGGGTGGGG CAGCCGAGCG GAGCGTGTGC 
ACCGATAAGC CCAGCCCTGG GGAGCGCCCC AGCTTGTCCC AACATCGGCG CCATCAGAGC 
TGGAGCACCT TCAAATCTAT GACACTGGGC TCGCTGCCCC CCAAGCCCCG AGAACGGCTG 
ACTCTGCACC GGGCAGCAGC CTGGGAGCCC ACAGAACCAC CGGATGGTGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( NM_001364857.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351786.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 3, mRNA.

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CloneID	OHu56448
Clone ID Related Accession (Same CDS sequence)	NM_001364857.1 , XM_011541848.3 , XM_011541848.2 , NM_001364857.2 , XM_017001899.1
Accession Version	NM_001364857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-29
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform 3 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354919.16 and AC114488.3. On Nov 30, 2019 this sequence version replaced NM_001364857.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351786.1
CDS	648..5405
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001364857.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364857.2

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001902.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857391.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu90315
Clone ID Related Accession (Same CDS sequence)	XM_017001902.1
Accession Version	XM_017001902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGGTGA TGCACACCCG GAAACGGCAT 
TCAGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG 
TCCACGGCCA AGGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857391.1
CDS	428..5125
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001902.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001902.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGGTGA TGCACACCCG GAAACGGCAT 
TCAGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG 
TCCACGGCCA AGGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001903.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857392.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.

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CloneID	OHu90316
Clone ID Related Accession (Same CDS sequence)	XM_017001903.1
Accession Version	XM_017001903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGG TGTCCCCTGG CTCTGTGCAC CTGCTCCGTG TCGTGGAGGA CTTCATTCAC 
CTGGTGGGCG ATGCTCTCAA GGCCTTCCAG AGCTCTCTGA TTGTCACAGA TAATCTAGTG 
ATCAGCATTC AGCGAGAGCC CGTCTCAGCT GTGTCCAGTG ACATCACGTT CCCCATGCGG 
GGCCGCCGGG GCATGAAGGA CTGGGTGCGG CACTCAGAGG ACCGCCTCTT CCTGCCCAAG 
GAGGTGCTCA GCCTCTCCTC CCCAGGGAAG CCAGCCACAT CTGGGGCAGC AGGCAGCCCT 
GGCAGGGGGA GGGGCCCAGG AACGGTGCCT CCTGGCCCAG GCCACTCCCA CCAGCGCCTC 
CTCCCAGCAG ACCCTGATGA GTCCTCCTAC TTTGTGATCG GTGCTGTACT CTACCGCACC 
CTTGGCCTCA TCCTGCCGCC TCCCAGGCCC CCGCTGGCCG TCACATCCCG GGTGATGACA 
GTGACTGTGC GCCCCCCTAC CCAGCCTCCA GCTGAGCCCC TCATCACTGT GGAGCTCTCC 
TACATCATCA ATGGGACCAC GGATCCCCAT TGCGCCAGCT GGGACTACTC CAGAGCAGAT 
GCCAGCTCAG GAGACTGGGA CACTGAAAAT TGCCAGACCC TGGAGACCCA GGCAGCTCAC 
ACCCGCTGCC AGTGCCAGCA CCTGTCCACC TTTGCTGTAC TAGCCCAGCC GCCCAAGGAC 
CTGACCCTGG AGCTGGCGGG CTCCCCCTCG GTCCCCCTGG TGATCGGCTG TGCAGTGTCG 
TGCATGGCGC TGCTCACCCT GCTCGCCATC TATGCCGCCT TTTGGAGGTT CATAAAATCT 
GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCT TGGCATCCAA CATCCTGATC 
CTCGTGGGCC AGTCCCGGGT GCTGAGCAAG GGCGTGTGCA CCATGACGGC TGCCTTCCTG 
CACTTCTTCT TTCTCTCCTC CTTTTGCTGG GTGCTTACCG AGGCCTGGCA GTCCTACCTG 
GCTGTCATTG GGCGGATGCG CACCCGCCTC GTTCGCAAGC GCTTCCTCTG CCTGGGCTGG 
GGTCTGCCTG CCCTGGTGGT GGCCGTGTCT GTTGGCTTTA CCCGAACGAA AGGATACGGT 
ACATCCAGCT ACTGCTGGCT CTCCCTGGAG GGCGGCCTGC TCTACGCCTT TGTGGGCCCT 
GCAGCCGTCA TTGTCCTGGT GAACATGCTC ATCGGAATCA TCGTCTTCAA CAAGCTCATG 
GCACGTGATG GCATCTCCGA CAAATCCAAG AAGCAGAGGG CCGGGTCGGA GCGGTGCCCC 
TGGGCCAGCC TGCTCCTCCC CTGCTCAGCG TGTGGAGCGG TCCCCAGCCC CCTGCTCAGC 
TCAGCCTCGG CCAGGAACGC CATGGCCTCA CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG 
CTGGCGCTCA CCTGGATGTC TGCCGTCCTG GCTATGACAG ACCGCCGTTC CGTCCTCTTC 
CAGGCCCTCT TTGCTGTCTT CAACTCCGCG CAGGGCTTTG TCATCACTGC TGTGCACTGC 
TTCCTGCGCC GAGAGGTCCA GGATGTGGTG AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT 
GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG AACGGGCAGC TGCAGATCCT GTCAGACTTT 
GAAAAGGATG TGGATCTGGC TTGTCAAACA GTGCTGTTCA AGGAGGTCAA CACTTGCAAC 
CCGTCCACCA TCACGGGCAC ACTATCCCGC CTGTCCCTGG ATGAGGATGA GGAGCCCAAG 
TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC AGCTTCTCAC CACTGCCTGG GAATATCCTG 
GTGCCCATGG CAGCCTCACC AGGGCTGGGG GAGCCTCCGC CCCCACAGGA GGCCAACCCT 
GTTTACATGT GTGGGGAGGG TGGCCTGCGG CAGCTGGACC TCACATGGCT GCGGCCCACT 
GAGCCAGGCT CTGAGGGAGA CTACATGGTG CTGCCCCGGC GGACTTTGAG CCTGCAGCCT 
GGCGGTGGGG GTGGAGGTGG TGAGGATGCC CCCAGGGCCC GGCCGGAGGG GACCCCCCGG 
CGAGCTGCCA AGACAGTGGC CCACACTGAA GGCTACCCCA GCTTCCTGTC CGTGGACCAC 
TCGGGCCTGG GGCTGGGCCC TGCCTATGGA TCTCTCCAGA ATCCCTATGG AATGACCTTC 
CAACCGCCAC CGCCGACACC CAGCGCCCGC CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG 
ACCATGCCTC GCACCGTGCC CGGCTCTACC ATGAAGATGG GCTCCCTGGA GCGAAAGAAA 
TTACGGTATT CAGACCTGGA CTTTGAGAAG GTGATGCACA CCCGGAAACG GCATTCAGAA 
CTCTACCACG AGCTCAACCA GAAGTTCCAC ACTTTCGACC GCTACCGCAG CCAGTCCACG 
GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC TCGGGTGGGG CAGCCGAGCG GAGCGTGTGC 
ACCGATAAGC CCAGCCCTGG GGAGCGCCCC AGCTTGTCCC AACATCGGCG CCATCAGAGC 
TGGAGCACCT TCAAATCTAT GACACTGGGC TCGCTGCCCC CCAAGCCCCG AGAACGGCTG 
ACTCTGCACC GGGCAGCAGC CTGGGAGCCC ACAGAACCAC CGGATGGTGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857392.1
CDS	428..5116
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001903.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001903.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGG TGTCCCCTGG CTCTGTGCAC CTGCTCCGTG TCGTGGAGGA CTTCATTCAC 
CTGGTGGGCG ATGCTCTCAA GGCCTTCCAG AGCTCTCTGA TTGTCACAGA TAATCTAGTG 
ATCAGCATTC AGCGAGAGCC CGTCTCAGCT GTGTCCAGTG ACATCACGTT CCCCATGCGG 
GGCCGCCGGG GCATGAAGGA CTGGGTGCGG CACTCAGAGG ACCGCCTCTT CCTGCCCAAG 
GAGGTGCTCA GCCTCTCCTC CCCAGGGAAG CCAGCCACAT CTGGGGCAGC AGGCAGCCCT 
GGCAGGGGGA GGGGCCCAGG AACGGTGCCT CCTGGCCCAG GCCACTCCCA CCAGCGCCTC 
CTCCCAGCAG ACCCTGATGA GTCCTCCTAC TTTGTGATCG GTGCTGTACT CTACCGCACC 
CTTGGCCTCA TCCTGCCGCC TCCCAGGCCC CCGCTGGCCG TCACATCCCG GGTGATGACA 
GTGACTGTGC GCCCCCCTAC CCAGCCTCCA GCTGAGCCCC TCATCACTGT GGAGCTCTCC 
TACATCATCA ATGGGACCAC GGATCCCCAT TGCGCCAGCT GGGACTACTC CAGAGCAGAT 
GCCAGCTCAG GAGACTGGGA CACTGAAAAT TGCCAGACCC TGGAGACCCA GGCAGCTCAC 
ACCCGCTGCC AGTGCCAGCA CCTGTCCACC TTTGCTGTAC TAGCCCAGCC GCCCAAGGAC 
CTGACCCTGG AGCTGGCGGG CTCCCCCTCG GTCCCCCTGG TGATCGGCTG TGCAGTGTCG 
TGCATGGCGC TGCTCACCCT GCTCGCCATC TATGCCGCCT TTTGGAGGTT CATAAAATCT 
GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCT TGGCATCCAA CATCCTGATC 
CTCGTGGGCC AGTCCCGGGT GCTGAGCAAG GGCGTGTGCA CCATGACGGC TGCCTTCCTG 
CACTTCTTCT TTCTCTCCTC CTTTTGCTGG GTGCTTACCG AGGCCTGGCA GTCCTACCTG 
GCTGTCATTG GGCGGATGCG CACCCGCCTC GTTCGCAAGC GCTTCCTCTG CCTGGGCTGG 
GGTCTGCCTG CCCTGGTGGT GGCCGTGTCT GTTGGCTTTA CCCGAACGAA AGGATACGGT 
ACATCCAGCT ACTGCTGGCT CTCCCTGGAG GGCGGCCTGC TCTACGCCTT TGTGGGCCCT 
GCAGCCGTCA TTGTCCTGGT GAACATGCTC ATCGGAATCA TCGTCTTCAA CAAGCTCATG 
GCACGTGATG GCATCTCCGA CAAATCCAAG AAGCAGAGGG CCGGGTCGGA GCGGTGCCCC 
TGGGCCAGCC TGCTCCTCCC CTGCTCAGCG TGTGGAGCGG TCCCCAGCCC CCTGCTCAGC 
TCAGCCTCGG CCAGGAACGC CATGGCCTCA CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG 
CTGGCGCTCA CCTGGATGTC TGCCGTCCTG GCTATGACAG ACCGCCGTTC CGTCCTCTTC 
CAGGCCCTCT TTGCTGTCTT CAACTCCGCG CAGGGCTTTG TCATCACTGC TGTGCACTGC 
TTCCTGCGCC GAGAGGTCCA GGATGTGGTG AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT 
GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG AACGGGCAGC TGCAGATCCT GTCAGACTTT 
GAAAAGGATG TGGATCTGGC TTGTCAAACA GTGCTGTTCA AGGAGGTCAA CACTTGCAAC 
CCGTCCACCA TCACGGGCAC ACTATCCCGC CTGTCCCTGG ATGAGGATGA GGAGCCCAAG 
TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC AGCTTCTCAC CACTGCCTGG GAATATCCTG 
GTGCCCATGG CAGCCTCACC AGGGCTGGGG GAGCCTCCGC CCCCACAGGA GGCCAACCCT 
GTTTACATGT GTGGGGAGGG TGGCCTGCGG CAGCTGGACC TCACATGGCT GCGGCCCACT 
GAGCCAGGCT CTGAGGGAGA CTACATGGTG CTGCCCCGGC GGACTTTGAG CCTGCAGCCT 
GGCGGTGGGG GTGGAGGTGG TGAGGATGCC CCCAGGGCCC GGCCGGAGGG GACCCCCCGG 
CGAGCTGCCA AGACAGTGGC CCACACTGAA GGCTACCCCA GCTTCCTGTC CGTGGACCAC 
TCGGGCCTGG GGCTGGGCCC TGCCTATGGA TCTCTCCAGA ATCCCTATGG AATGACCTTC 
CAACCGCCAC CGCCGACACC CAGCGCCCGC CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG 
ACCATGCCTC GCACCGTGCC CGGCTCTACC ATGAAGATGG GCTCCCTGGA GCGAAAGAAA 
TTACGGTATT CAGACCTGGA CTTTGAGAAG GTGATGCACA CCCGGAAACG GCATTCAGAA 
CTCTACCACG AGCTCAACCA GAAGTTCCAC ACTTTCGACC GCTACCGCAG CCAGTCCACG 
GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC TCGGGTGGGG CAGCCGAGCG GAGCGTGTGC 
ACCGATAAGC CCAGCCCTGG GGAGCGCCCC AGCTTGTCCC AACATCGGCG CCATCAGAGC 
TGGAGCACCT TCAAATCTAT GACACTGGGC TCGCTGCCCC CCAAGCCCCG AGAACGGCTG 
ACTCTGCACC GGGCAGCAGC CTGGGAGCCC ACAGAACCAC CGGATGGTGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001906.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857395.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.

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CloneID	OHu90317
Clone ID Related Accession (Same CDS sequence)	XM_017001906.1
Accession Version	XM_017001906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGAAG 
GTGATGCACA CCCGGAAACG GCATTCAGAA CTCTACCACG AGCTCAACCA GAAGTTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCCACG GCCAAGGATA AGCCCAGCCC TGGGGAGCGC 
CCCAGCTTGT CCCAACATCG GCGCCATCAG AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGACTCTGC ACCGGGCAGC AGCCTGGGAG 
CCCACAGAAC CACCGGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857395.1
CDS	425..5026
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001906.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001906.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGAAG 
GTGATGCACA CCCGGAAACG GCATTCAGAA CTCTACCACG AGCTCAACCA GAAGTTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCCACG GCCAAGGATA AGCCCAGCCC TGGGGAGCGC 
CCCAGCTTGT CCCAACATCG GCGCCATCAG AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGACTCTGC ACCGGGCAGC AGCCTGGGAG 
CCCACAGAAC CACCGGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( NM_001294335.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001281264.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 1, mRNA.

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CloneID	OHu28697
Clone ID Related Accession (Same CDS sequence)	NM_001294335.2 , NM_001294335.1 , XM_017001900.1
Accession Version	NM_001294335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354919.16, DA494051.1, AB005298.1, AC114488.3 and AI291691.1. On Jun 2, 2019 this sequence version replaced NM_001294335.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB005298.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGGTGA TGCACACCCG GAAACGGCAT 
TCAGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG 
TCCACGGCCA AGAGGGAGAA GCGGTGGAGT GTGTCCTCGG GTGGGGCAGC CGAGCGGAGC 
GTGTGCACCG ATAAGCCCAG CCCTGGGGAG CGCCCCAGCT TGTCCCAACA TCGGCGCCAT 
CAGAGCTGGA GCACCTTCAA ATCTATGACA CTGGGCTCGC TGCCCCCCAA GCCCCGAGAA 
CGGCTGACTC TGCACCGGGC AGCAGCCTGG GAGCCCACAG AACCACCGGA TGGTGACTTC 
CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001281264.1
CDS	648..5402
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001294335.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001294335.2

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGGTGA TGCACACCCG GAAACGGCAT 
TCAGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG 
TCCACGGCCA AGAGGGAGAA GCGGTGGAGT GTGTCCTCGG GTGGGGCAGC CGAGCGGAGC 
GTGTGCACCG ATAAGCCCAG CCCTGGGGAG CGCCCCAGCT TGTCCCAACA TCGGCGCCAT 
CAGAGCTGGA GCACCTTCAA ATCTATGACA CTGGGCTCGC TGCCCCCCAA GCCCCGAGAA 
CGGCTGACTC TGCACCGGGC AGCAGCCTGG GAGCCCACAG AACCACCGGA TGGTGACTTC 
CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( NM_001294335.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001281264.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 1, mRNA.

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CloneID	OHu28697
Clone ID Related Accession (Same CDS sequence)	NM_001294335.2 , NM_001294335.1 , XM_017001900.1
Accession Version	NM_001294335.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA494051.1, AB005298.1, AC114488.3 and AI291691.1. On Jul 1, 2014 this sequence version replaced XM_006710783.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB005298.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGGTGA TGCACACCCG GAAACGGCAT 
TCAGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG 
TCCACGGCCA AGAGGGAGAA GCGGTGGAGT GTGTCCTCGG GTGGGGCAGC CGAGCGGAGC 
GTGTGCACCG ATAAGCCCAG CCCTGGGGAG CGCCCCAGCT TGTCCCAACA TCGGCGCCAT 
CAGAGCTGGA GCACCTTCAA ATCTATGACA CTGGGCTCGC TGCCCCCCAA GCCCCGAGAA 
CGGCTGACTC TGCACCGGGC AGCAGCCTGG GAGCCCACAG AACCACCGGA TGGTGACTTC 
CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001281264.1
CDS	371..5125
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001294335.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001294335.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGGTGA TGCACACCCG GAAACGGCAT 
TCAGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG 
TCCACGGCCA AGAGGGAGAA GCGGTGGAGT GTGTCCTCGG GTGGGGCAGC CGAGCGGAGC 
GTGTGCACCG ATAAGCCCAG CCCTGGGGAG CGCCCCAGCT TGTCCCAACA TCGGCGCCAT 
CAGAGCTGGA GCACCTTCAA ATCTATGACA CTGGGCTCGC TGCCCCCCAA GCCCCGAGAA 
CGGCTGACTC TGCACCGGGC AGCAGCCTGG GAGCCCACAG AACCACCGGA TGGTGACTTC 
CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( NM_001294336.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001281265.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28698
Clone ID Related Accession (Same CDS sequence)	NM_001294336.2 , XM_017001905.1 , NM_001294336.1
Accession Version	NM_001294336.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354919.16, DA494051.1, AB005298.1, BC136533.1, AC114488.3 and AI291691.1. On Jun 2, 2019 this sequence version replaced NM_001294336.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## CDS exon combination :: BC136533.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGGTG 
ATGCACACCC GGAAACGGCA TTCAGAACTC TACCACGAGC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGCAGCCA GTCCACGGCC AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG 
GGTGGGGCAG CCGAGCGGAG CGTGTGCACC GATAAGCCCA GCCCTGGGGA GCGCCCCAGC 
TTGTCCCAAC ATCGGCGCCA TCAGAGCTGG AGCACCTTCA AATCTATGAC ACTGGGCTCG 
CTGCCCCCCA AGCCCCGAGA ACGGCTGACT CTGCACCGGG CAGCAGCCTG GGAGCCCACA 
GAACCACCGG ATGGTGACTT CCAGACAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001281265.1
CDS	648..5303
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001294336.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001294336.2

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGGTG 
ATGCACACCC GGAAACGGCA TTCAGAACTC TACCACGAGC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGCAGCCA GTCCACGGCC AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG 
GGTGGGGCAG CCGAGCGGAG CGTGTGCACC GATAAGCCCA GCCCTGGGGA GCGCCCCAGC 
TTGTCCCAAC ATCGGCGCCA TCAGAGCTGG AGCACCTTCA AATCTATGAC ACTGGGCTCG 
CTGCCCCCCA AGCCCCGAGA ACGGCTGACT CTGCACCGGG CAGCAGCCTG GGAGCCCACA 
GAACCACCGG ATGGTGACTT CCAGACAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( NM_001294336.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001281265.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28698
Clone ID Related Accession (Same CDS sequence)	NM_001294336.2 , XM_017001905.1 , NM_001294336.1
Accession Version	NM_001294336.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA494051.1, AB005298.1, BC136533.1, AC114488.3 and AI291691.1. On Jul 1, 2014 this sequence version replaced XM_005271067.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## CDS exon combination :: BC136533.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGGTG 
ATGCACACCC GGAAACGGCA TTCAGAACTC TACCACGAGC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGCAGCCA GTCCACGGCC AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG 
GGTGGGGCAG CCGAGCGGAG CGTGTGCACC GATAAGCCCA GCCCTGGGGA GCGCCCCAGC 
TTGTCCCAAC ATCGGCGCCA TCAGAGCTGG AGCACCTTCA AATCTATGAC ACTGGGCTCG 
CTGCCCCCCA AGCCCCGAGA ACGGCTGACT CTGCACCGGG CAGCAGCCTG GGAGCCCACA 
GAACCACCGG ATGGTGACTT CCAGACAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001281265.1
CDS	371..5026
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001294336.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001294336.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGGTG 
ATGCACACCC GGAAACGGCA TTCAGAACTC TACCACGAGC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGCAGCCA GTCCACGGCC AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG 
GGTGGGGCAG CCGAGCGGAG CGTGTGCACC GATAAGCCCA GCCCTGGGGA GCGCCCCAGC 
TTGTCCCAAC ATCGGCGCCA TCAGAGCTGG AGCACCTTCA AATCTATGAC ACTGGGCTCG 
CTGCCCCCCA AGCCCCGAGA ACGGCTGACT CTGCACCGGG CAGCAGCCTG GGAGCCCACA 
GAACCACCGG ATGGTGACTT CCAGACAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( NM_001364857.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351786.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 3, mRNA.

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CloneID	OHu56448
Clone ID Related Accession (Same CDS sequence)	NM_001364857.1 , XM_011541848.3 , XM_011541848.2 , NM_001364857.2 , XM_017001899.1
Accession Version	NM_001364857.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform 3 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL354919.16 and AC114488.3. On Sep 25, 2018 this sequence version replaced NM_001703.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351786.1
CDS	358..5115
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001364857.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364857.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001900.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857389.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.

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CloneID	OHu28697
Clone ID Related Accession (Same CDS sequence)	NM_001294335.2 , NM_001294335.1 , XM_017001900.1
Accession Version	XM_017001900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGGTGA TGCACACCCG GAAACGGCAT 
TCAGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG 
TCCACGGCCA AGAGGGAGAA GCGGTGGAGT GTGTCCTCGG GTGGGGCAGC CGAGCGGAGC 
GTGTGCACCG ATAAGCCCAG CCCTGGGGAG CGCCCCAGCT TGTCCCAACA TCGGCGCCAT 
CAGAGCTGGA GCACCTTCAA ATCTATGACA CTGGGCTCGC TGCCCCCCAA GCCCCGAGAA 
CGGCTGACTC TGCACCGGGC AGCAGCCTGG GAGCCCACAG AACCACCGGA TGGTGACTTC 
CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857389.1
CDS	427..5181
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001900.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001900.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGGTGA TGCACACCCG GAAACGGCAT 
TCAGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG 
TCCACGGCCA AGAGGGAGAA GCGGTGGAGT GTGTCCTCGG GTGGGGCAGC CGAGCGGAGC 
GTGTGCACCG ATAAGCCCAG CCCTGGGGAG CGCCCCAGCT TGTCCCAACA TCGGCGCCAT 
CAGAGCTGGA GCACCTTCAA ATCTATGACA CTGGGCTCGC TGCCCCCCAA GCCCCGAGAA 
CGGCTGACTC TGCACCGGGC AGCAGCCTGG GAGCCCACAG AACCACCGGA TGGTGACTTC 
CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001905.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857394.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.

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CloneID	OHu28698
Clone ID Related Accession (Same CDS sequence)	NM_001294336.2 , XM_017001905.1 , NM_001294336.1
Accession Version	XM_017001905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGGTG 
ATGCACACCC GGAAACGGCA TTCAGAACTC TACCACGAGC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGCAGCCA GTCCACGGCC AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG 
GGTGGGGCAG CCGAGCGGAG CGTGTGCACC GATAAGCCCA GCCCTGGGGA GCGCCCCAGC 
TTGTCCCAAC ATCGGCGCCA TCAGAGCTGG AGCACCTTCA AATCTATGAC ACTGGGCTCG 
CTGCCCCCCA AGCCCCGAGA ACGGCTGACT CTGCACCGGG CAGCAGCCTG GGAGCCCACA 
GAACCACCGG ATGGTGACTT CCAGACAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857394.1
CDS	426..5081
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001905.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001905.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGGTG 
ATGCACACCC GGAAACGGCA TTCAGAACTC TACCACGAGC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGCAGCCA GTCCACGGCC AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG 
GGTGGGGCAG CCGAGCGGAG CGTGTGCACC GATAAGCCCA GCCCTGGGGA GCGCCCCAGC 
TTGTCCCAAC ATCGGCGCCA TCAGAGCTGG AGCACCTTCA AATCTATGAC ACTGGGCTCG 
CTGCCCCCCA AGCCCCGAGA ACGGCTGACT CTGCACCGGG CAGCAGCCTG GGAGCCCACA 
GAACCACCGG ATGGTGACTT CCAGACAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001901.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857390.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu33641
Clone ID Related Accession (Same CDS sequence)	XM_017001901.1
Accession Version	XM_017001901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGGATAA GCCCAGCCCT GGGGAGCGCC CCAGCTTGTC CCAACATCGG 
CGCCATCAGA GCTGGAGCAC CTTCAAATCT ATGACACTGG GCTCGCTGCC CCCCAAGCCC 
CGAGAACGGC TGACTCTGCA CCGGGCAGCA GCCTGGGAGC CCACAGAACC ACCGGATGGT 
GACTTCCAGA CAGAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857390.1
CDS	427..5127
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001901.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001901.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGGATAA GCCCAGCCCT GGGGAGCGCC CCAGCTTGTC CCAACATCGG 
CGCCATCAGA GCTGGAGCAC CTTCAAATCT ATGACACTGG GCTCGCTGCC CCCCAAGCCC 
CGAGAACGGC TGACTCTGCA CCGGGCAGCA GCCTGGGAGC CCACAGAACC ACCGGATGGT 
GACTTCCAGA CAGAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001904.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857393.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu33642
Clone ID Related Accession (Same CDS sequence)	XM_017001904.1
Accession Version	XM_017001904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGAAG 
GTGATGCACA CCCGGAAACG GCATTCAGAA CTCTACCACG AGCTCAACCA GAAGTTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCCACG GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC 
TCGGGTGGGG CAGCCGAGCG GAGCGTGTGC ACCGATAAGC CCAGCCCTGG GGAGCGCCCC 
AGCTTGTCCC AACATCGGCG CCATCAGAGC TGGAGCACCT TCAAATCTAT GACACTGGGC 
TCGCTGCCCC CCAAGCCCCG AGAACGGCTG ACTCTGCACC GGGCAGCAGC CTGGGAGCCC 
ACAGAACCAC CGGATGGTGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857393.1
CDS	424..5082
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001904.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001904.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGGCCTCA 
CTCTGGAGCT CCTGCGTGGT GCTGCCCCTG CTGGCGCTCA CCTGGATGTC TGCCGTCCTG 
GCTATGACAG ACCGCCGTTC CGTCCTCTTC CAGGCCCTCT TTGCTGTCTT CAACTCCGCG 
CAGGGCTTTG TCATCACTGC TGTGCACTGC TTCCTGCGCC GAGAGGTCCA GGATGTGGTG 
AAGTGCCAGA TGGGGGTGTG CCGGGCTGAT GAGAGCGAAG ACTCCCCTGA CTCGTGTAAG 
AACGGGCAGC TGCAGATCCT GTCAGACTTT GAAAAGGATG TGGATCTGGC TTGTCAAACA 
GTGCTGTTCA AGGAGGTCAA CACTTGCAAC CCGTCCACCA TCACGGGCAC ACTATCCCGC 
CTGTCCCTGG ATGAGGATGA GGAGCCCAAG TCCTGCCTCG TGGGCCCTGA GGGCAGCCTC 
AGCTTCTCAC CACTGCCTGG GAATATCCTG GTGCCCATGG CAGCCTCACC AGGGCTGGGG 
GAGCCTCCGC CCCCACAGGA GGCCAACCCT GTTTACATGT GTGGGGAGGG TGGCCTGCGG 
CAGCTGGACC TCACATGGCT GCGGCCCACT GAGCCAGGCT CTGAGGGAGA CTACATGGTG 
CTGCCCCGGC GGACTTTGAG CCTGCAGCCT GGCGGTGGGG GTGGAGGTGG TGAGGATGCC 
CCCAGGGCCC GGCCGGAGGG GACCCCCCGG CGAGCTGCCA AGACAGTGGC CCACACTGAA 
GGCTACCCCA GCTTCCTGTC CGTGGACCAC TCGGGCCTGG GGCTGGGCCC TGCCTATGGA 
TCTCTCCAGA ATCCCTATGG AATGACCTTC CAACCGCCAC CGCCGACACC CAGCGCCCGC 
CAAGTGCCCG AGCCAGGGGA GCGCAGCCGG ACCATGCCTC GCACCGTGCC CGGCTCTACC 
ATGAAGATGG GCTCCCTGGA GCGAAAGAAA TTACGGTATT CAGACCTGGA CTTTGAGAAG 
GTGATGCACA CCCGGAAACG GCATTCAGAA CTCTACCACG AGCTCAACCA GAAGTTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCCACG GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC 
TCGGGTGGGG CAGCCGAGCG GAGCGTGTGC ACCGATAAGC CCAGCCCTGG GGAGCGCCCC 
AGCTTGTCCC AACATCGGCG CCATCAGAGC TGGAGCACCT TCAAATCTAT GACACTGGGC 
TCGCTGCCCC CCAAGCCCCG AGAACGGCTG ACTCTGCACC GGGCAGCAGC CTGGGAGCCC 
ACAGAACCAC CGGATGGTGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001907.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857396.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA.

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CloneID	OHu33643
Clone ID Related Accession (Same CDS sequence)	XM_017001907.1
Accession Version	XM_017001907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGT CGGAGCGGTG CCCCTGGGCC AGCCTGCTCC TCCCCTGCTC AGCGTGTGGA 
GCGGTCCCCA GCCCCCTGCT CAGCTCAGCC TCGGCCAGGA ACGCCATGGC CTCACTCTGG 
AGCTCCTGCG TGGTGCTGCC CCTGCTGGCG CTCACCTGGA TGTCTGCCGT CCTGGCTATG 
ACAGACCGCC GTTCCGTCCT CTTCCAGGCC CTCTTTGCTG TCTTCAACTC CGCGCAGGGC 
TTTGTCATCA CTGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTGAAGTGC 
CAGATGGGGG TGTGCCGGGC TGATGAGAGC GAAGACTCCC CTGACTCGTG TAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GATGTGGATC TGGCTTGTCA AACAGTGCTG 
TTCAAGGAGG TCAACACTTG CAACCCGTCC ACCATCACGG GCACACTATC CCGCCTGTCC 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGC CTCGTGGGCC CTGAGGGCAG CCTCAGCTTC 
TCACCACTGC CTGGGAATAT CCTGGTGCCC ATGGCAGCCT CACCAGGGCT GGGGGAGCCT 
CCGCCCCCAC AGGAGGCCAA CCCTGTTTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGCTG 
GACCTCACAT GGCTGCGGCC CACTGAGCCA GGCTCTGAGG GAGACTACAT GGTGCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGCGGT GGGGGTGGAG GTGGTGAGGA TGCCCCCAGG 
GCCCGGCCGG AGGGGACCCC CCGGCGAGCT GCCAAGACAG TGGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCCGTGGA CCACTCGGGC CTGGGGCTGG GCCCTGCCTA TGGATCTCTC 
CAGAATCCCT ATGGAATGAC CTTCCAACCG CCACCGCCGA CACCCAGCGC CCGCCAAGTG 
CCCGAGCCAG GGGAGCGCAG CCGGACCATG CCTCGCACCG TGCCCGGCTC TACCATGAAG 
ATGGGCTCCC TGGAGCGAAA GAAATTACGG TATTCAGACC TGGACTTTGA GAAGGTGATG 
CACACCCGGA AACGGCATTC AGAACTCTAC CACGAGCTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GCAGCCAGTC CACGGCCAAG AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT 
GGGGCAGCCG AGCGGAGCGT GTGCACCGAT AAGCCCAGCC CTGGGGAGCG CCCCAGCTTG 
TCCCAACATC GGCGCCATCA GAGCTGGAGC ACCTTCAAAT CTATGACACT GGGCTCGCTG 
CCCCCCAAGC CCCGAGAACG GCTGACTCTG CACCGGGCAG CAGCCTGGGA GCCCACAGAA 
CCACCGGATG GTGACTTCCA GACAGAGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857396.1
CDS	424..5016
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001907.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001907.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGT CGGAGCGGTG CCCCTGGGCC AGCCTGCTCC TCCCCTGCTC AGCGTGTGGA 
GCGGTCCCCA GCCCCCTGCT CAGCTCAGCC TCGGCCAGGA ACGCCATGGC CTCACTCTGG 
AGCTCCTGCG TGGTGCTGCC CCTGCTGGCG CTCACCTGGA TGTCTGCCGT CCTGGCTATG 
ACAGACCGCC GTTCCGTCCT CTTCCAGGCC CTCTTTGCTG TCTTCAACTC CGCGCAGGGC 
TTTGTCATCA CTGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTGAAGTGC 
CAGATGGGGG TGTGCCGGGC TGATGAGAGC GAAGACTCCC CTGACTCGTG TAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GATGTGGATC TGGCTTGTCA AACAGTGCTG 
TTCAAGGAGG TCAACACTTG CAACCCGTCC ACCATCACGG GCACACTATC CCGCCTGTCC 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGC CTCGTGGGCC CTGAGGGCAG CCTCAGCTTC 
TCACCACTGC CTGGGAATAT CCTGGTGCCC ATGGCAGCCT CACCAGGGCT GGGGGAGCCT 
CCGCCCCCAC AGGAGGCCAA CCCTGTTTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGCTG 
GACCTCACAT GGCTGCGGCC CACTGAGCCA GGCTCTGAGG GAGACTACAT GGTGCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGCGGT GGGGGTGGAG GTGGTGAGGA TGCCCCCAGG 
GCCCGGCCGG AGGGGACCCC CCGGCGAGCT GCCAAGACAG TGGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCCGTGGA CCACTCGGGC CTGGGGCTGG GCCCTGCCTA TGGATCTCTC 
CAGAATCCCT ATGGAATGAC CTTCCAACCG CCACCGCCGA CACCCAGCGC CCGCCAAGTG 
CCCGAGCCAG GGGAGCGCAG CCGGACCATG CCTCGCACCG TGCCCGGCTC TACCATGAAG 
ATGGGCTCCC TGGAGCGAAA GAAATTACGG TATTCAGACC TGGACTTTGA GAAGGTGATG 
CACACCCGGA AACGGCATTC AGAACTCTAC CACGAGCTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GCAGCCAGTC CACGGCCAAG AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT 
GGGGCAGCCG AGCGGAGCGT GTGCACCGAT AAGCCCAGCC CTGGGGAGCG CCCCAGCTTG 
TCCCAACATC GGCGCCATCA GAGCTGGAGC ACCTTCAAAT CTATGACACT GGGCTCGCTG 
CCCCCCAAGC CCCGAGAACG GCTGACTCTG CACCGGGCAG CAGCCTGGGA GCCCACAGAA 
CCACCGGATG GTGACTTCCA GACAGAGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001908.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857397.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X13, mRNA.

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CloneID	OHu33644
Clone ID Related Accession (Same CDS sequence)	XM_017001908.1
Accession Version	XM_017001908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGCACGGC GTGTGGGAGG AGTGGGGGTC CTGGAGCCTG 
TGCTCCCGCA GCTGCGGGCG GGGGTCCCGG AGCCGGATGC GGACCTGCGT GCCCCCCCAG 
CACGGCGGCA AGGCCTGCGA GGGTCCTGAG CTGCAGACTA AGCTCTGCAG TATGGCTGCC 
TGCCCGGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGT CGGAGCGGTG CCCCTGGGCC AGCCTGCTCC TCCCCTGCTC AGCGTGTGGA 
GCGGTCCCCA GCCCCCTGCT CAGCTCAGCC TCGGCCAGGA ACGCCATGGC CTCACTCTGG 
AGCTCCTGCG TGGTGCTGCC CCTGCTGGCG CTCACCTGGA TGTCTGCCGT CCTGGCTATG 
ACAGACCGCC GTTCCGTCCT CTTCCAGGCC CTCTTTGCTG TCTTCAACTC CGCGCAGGGC 
TTTGTCATCA CTGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTGAAGTGC 
CAGATGGGGG TGTGCCGGGC TGATGAGAGC GAAGACTCCC CTGACTCGTG TAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GATGTGGATC TGGCTTGTCA AACAGTGCTG 
TTCAAGGAGG TCAACACTTG CAACCCGTCC ACCATCACGG GCACACTATC CCGCCTGTCC 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGC CTCGTGGGCC CTGAGGGCAG CCTCAGCTTC 
TCACCACTGC CTGGGAATAT CCTGGTGCCC ATGGCAGCCT CACCAGGGCT GGGGGAGCCT 
CCGCCCCCAC AGGAGGCCAA CCCTGTTTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGCTG 
GACCTCACAT GGCTGCGGCC CACTGAGCCA GGCTCTGAGG GAGACTACAT GGTGCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGCGGT GGGGGTGGAG GTGGTGAGGA TGCCCCCAGG 
GCCCGGCCGG AGGGGACCCC CCGGCGAGCT GCCAAGACAG TGGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCCGTGGA CCACTCGGGC CTGGGGCTGG GCCCTGCCTA TGGATCTCTC 
CAGAATCCCT ATGGAATGAC CTTCCAACCG CCACCGCCGA CACCCAGCGC CCGCCAAGTG 
CCCGAGCCAG GGGAGCGCAG CCGGACCATG CCTCGCACCG TGCCCGGCTC TACCATGAAG 
ATGGGCTCCC TGGAGCGAAA GAAATTACGG TATTCAGACC TGGACTTTGA GAAGGTGATG 
CACACCCGGA AACGGCATTC AGAACTCTAC CACGAGCTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GCAGCCAGTC CACGGCCAAG AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT 
GGGGCAGCCG AGCGGAGCGT GTGCACCGAT AAGCCCAGCC CTGGGGAGCG CCCCAGCTTG 
TCCCAACATC GGCGCCATCA GAGCTGGAGC ACCTTCAAAT CTATGACACT GGGCTCGCTG 
CCCCCCAAGC CCCGAGAACG GCTGACTCTG CACCGGGCAG CAGCCTGGGA GCCCACAGAA 
CCACCGGATG GTGACTTCCA GACAGAGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857397.1
CDS	425..5017
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001908.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001908.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGCACGGC GTGTGGGAGG AGTGGGGGTC CTGGAGCCTG 
TGCTCCCGCA GCTGCGGGCG GGGGTCCCGG AGCCGGATGC GGACCTGCGT GCCCCCCCAG 
CACGGCGGCA AGGCCTGCGA GGGTCCTGAG CTGCAGACTA AGCTCTGCAG TATGGCTGCC 
TGCCCGGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGT CGGAGCGGTG CCCCTGGGCC AGCCTGCTCC TCCCCTGCTC AGCGTGTGGA 
GCGGTCCCCA GCCCCCTGCT CAGCTCAGCC TCGGCCAGGA ACGCCATGGC CTCACTCTGG 
AGCTCCTGCG TGGTGCTGCC CCTGCTGGCG CTCACCTGGA TGTCTGCCGT CCTGGCTATG 
ACAGACCGCC GTTCCGTCCT CTTCCAGGCC CTCTTTGCTG TCTTCAACTC CGCGCAGGGC 
TTTGTCATCA CTGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTGAAGTGC 
CAGATGGGGG TGTGCCGGGC TGATGAGAGC GAAGACTCCC CTGACTCGTG TAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GATGTGGATC TGGCTTGTCA AACAGTGCTG 
TTCAAGGAGG TCAACACTTG CAACCCGTCC ACCATCACGG GCACACTATC CCGCCTGTCC 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGC CTCGTGGGCC CTGAGGGCAG CCTCAGCTTC 
TCACCACTGC CTGGGAATAT CCTGGTGCCC ATGGCAGCCT CACCAGGGCT GGGGGAGCCT 
CCGCCCCCAC AGGAGGCCAA CCCTGTTTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGCTG 
GACCTCACAT GGCTGCGGCC CACTGAGCCA GGCTCTGAGG GAGACTACAT GGTGCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGCGGT GGGGGTGGAG GTGGTGAGGA TGCCCCCAGG 
GCCCGGCCGG AGGGGACCCC CCGGCGAGCT GCCAAGACAG TGGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCCGTGGA CCACTCGGGC CTGGGGCTGG GCCCTGCCTA TGGATCTCTC 
CAGAATCCCT ATGGAATGAC CTTCCAACCG CCACCGCCGA CACCCAGCGC CCGCCAAGTG 
CCCGAGCCAG GGGAGCGCAG CCGGACCATG CCTCGCACCG TGCCCGGCTC TACCATGAAG 
ATGGGCTCCC TGGAGCGAAA GAAATTACGG TATTCAGACC TGGACTTTGA GAAGGTGATG 
CACACCCGGA AACGGCATTC AGAACTCTAC CACGAGCTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GCAGCCAGTC CACGGCCAAG AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT 
GGGGCAGCCG AGCGGAGCGT GTGCACCGAT AAGCCCAGCC CTGGGGAGCG CCCCAGCTTG 
TCCCAACATC GGCGCCATCA GAGCTGGAGC ACCTTCAAAT CTATGACACT GGGCTCGCTG 
CCCCCCAAGC CCCGAGAACG GCTGACTCTG CACCGGGCAG CAGCCTGGGA GCCCACAGAA 
CCACCGGATG GTGACTTCCA GACAGAGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001909.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857398.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X14, mRNA.

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CloneID	OHu33645
Clone ID Related Accession (Same CDS sequence)	XM_017001909.1
Accession Version	XM_017001909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGCACGGC GTGTGGGAGG AGTGGGGGTC CTGGAGCCTG 
TGCTCCCGCA GCTGCGGGCG GGGGTCCCGG AGCCGGATGC GGACCTGCGT GCCCCCCCAG 
CACGGCGGCA AGGCCTGCGA GGGTCCTGAG CTGCAGACTA AGCTCTGCAG TATGGCTGCC 
TGCCCGGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGT CGGAGCGGTG CCCCTGGGCC AGCCTGCTCC TCCCCTGCTC AGCGTGTGGA 
GCGGTCCCCA GCCCCCTGCT CAGCTCAGCC TCGGCCAGGA ACGCCATGGC CTCACTCTGG 
AGCTCCTGCG TGGTGCTGCC CCTGCTGGCG CTCACCTGGA TGTCTGCCGT CCTGGCTATG 
ACAGACCGCC GTTCCGTCCT CTTCCAGGCC CTCTTTGCTG TCTTCAACTC CGCGCAGGGC 
TTTGTCATCA CTGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTGAAGTGC 
CAGATGGGGG TGTGCCGGGC TGATGAGAGC GAAGACTCCC CTGACTCGTG TAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GATGTGGATC TGGCTTGTCA AACAGTGCTG 
TTCAAGGAGG TCAACACTTG CAACCCGTCC ACCATCACGG GCACACTATC CCGCCTGTCC 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGC CTCGTGGGCC CTGAGGGCAG CCTCAGCTTC 
TCACCACTGC CTGGGAATAT CCTGGTGCCC ATGGCAGCCT CACCAGGGCT GGGGGAGCCT 
CCGCCCCCAC AGGAGGCCAA CCCTGTTTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGCTG 
GACCTCACAT GGCTGCGGCC CACTGAGCCA GGCTCTGAGG GAGACTACAT GGTGCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGCGGT GGGGGTGGAG GTGGTGAGGA TGCCCCCAGG 
GCCCGGCCGG AGGGGACCCC CCGGCGAGCT GCCAAGACAG TGGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCCGTGGA CCACTCGGGC CTGGGGCTGG GCCCTGCCTA TGGATCTCTC 
CAGAATCCCT ATGGAATGAC CTTCCAACCG CCACCGCCGA CACCCAGCGC CCGCCAAGTG 
CCCGAGCCAG GGGAGCGCAG CCGGACCATG CCTCGCACCG TGCCCGGCTC TACCATGAAG 
ATGGGCTCCC TGGAGCGAAA GAAATTACGG TATTCAGACC TGGACTTTGA GAAGGTGATG 
CACACCCGGA AACGGCATTC AGAACTCTAC CACGAGCTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GCAGCCAGTC CACGGCCAAG GATAAGCCCA GCCCTGGGGA GCGCCCCAGC 
TTGTCCCAAC ATCGGCGCCA TCAGAGCTGG AGCACCTTCA AATCTATGAC ACTGGGCTCG 
CTGCCCCCCA AGCCCCGAGA ACGGCTGACT CTGCACCGGG CAGCAGCCTG GGAGCCCACA 
GAACCACCGG ATGGTGACTT CCAGACAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857398.1
CDS	426..4961
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001909.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001909.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGCACGGC GTGTGGGAGG AGTGGGGGTC CTGGAGCCTG 
TGCTCCCGCA GCTGCGGGCG GGGGTCCCGG AGCCGGATGC GGACCTGCGT GCCCCCCCAG 
CACGGCGGCA AGGCCTGCGA GGGTCCTGAG CTGCAGACTA AGCTCTGCAG TATGGCTGCC 
TGCCCGGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGT CGGAGCGGTG CCCCTGGGCC AGCCTGCTCC TCCCCTGCTC AGCGTGTGGA 
GCGGTCCCCA GCCCCCTGCT CAGCTCAGCC TCGGCCAGGA ACGCCATGGC CTCACTCTGG 
AGCTCCTGCG TGGTGCTGCC CCTGCTGGCG CTCACCTGGA TGTCTGCCGT CCTGGCTATG 
ACAGACCGCC GTTCCGTCCT CTTCCAGGCC CTCTTTGCTG TCTTCAACTC CGCGCAGGGC 
TTTGTCATCA CTGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTGAAGTGC 
CAGATGGGGG TGTGCCGGGC TGATGAGAGC GAAGACTCCC CTGACTCGTG TAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GATGTGGATC TGGCTTGTCA AACAGTGCTG 
TTCAAGGAGG TCAACACTTG CAACCCGTCC ACCATCACGG GCACACTATC CCGCCTGTCC 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGC CTCGTGGGCC CTGAGGGCAG CCTCAGCTTC 
TCACCACTGC CTGGGAATAT CCTGGTGCCC ATGGCAGCCT CACCAGGGCT GGGGGAGCCT 
CCGCCCCCAC AGGAGGCCAA CCCTGTTTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGCTG 
GACCTCACAT GGCTGCGGCC CACTGAGCCA GGCTCTGAGG GAGACTACAT GGTGCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGCGGT GGGGGTGGAG GTGGTGAGGA TGCCCCCAGG 
GCCCGGCCGG AGGGGACCCC CCGGCGAGCT GCCAAGACAG TGGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCCGTGGA CCACTCGGGC CTGGGGCTGG GCCCTGCCTA TGGATCTCTC 
CAGAATCCCT ATGGAATGAC CTTCCAACCG CCACCGCCGA CACCCAGCGC CCGCCAAGTG 
CCCGAGCCAG GGGAGCGCAG CCGGACCATG CCTCGCACCG TGCCCGGCTC TACCATGAAG 
ATGGGCTCCC TGGAGCGAAA GAAATTACGG TATTCAGACC TGGACTTTGA GAAGGTGATG 
CACACCCGGA AACGGCATTC AGAACTCTAC CACGAGCTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GCAGCCAGTC CACGGCCAAG GATAAGCCCA GCCCTGGGGA GCGCCCCAGC 
TTGTCCCAAC ATCGGCGCCA TCAGAGCTGG AGCACCTTCA AATCTATGAC ACTGGGCTCG 
CTGCCCCCCA AGCCCCGAGA ACGGCTGACT CTGCACCGGG CAGCAGCCTG GGAGCCCACA 
GAACCACCGG ATGGTGACTT CCAGACAGAG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001910.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857399.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu33646
Clone ID Related Accession (Same CDS sequence)	XM_017001910.1
Accession Version	XM_017001910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGCACGGC GTGTGGGAGG AGTGGGGGTC CTGGAGCCTG 
TGCTCCCGCA GCTGCGGGCG GGGGTCCCGG AGCCGGATGC GGACCTGCGT GCCCCCCCAG 
CACGGCGGCA AGGCCTGCGA GGGTCCTGAG CTGCAGACTA AGCTCTGCAG TATGGCTGCC 
TGCCCGGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGG CCTCACTCTG GAGCTCCTGC GTGGTGCTGC CCCTGCTGGC GCTCACCTGG 
ATGTCTGCCG TCCTGGCTAT GACAGACCGC CGTTCCGTCC TCTTCCAGGC CCTCTTTGCT 
GTCTTCAACT CCGCGCAGGG CTTTGTCATC ACTGCTGTGC ACTGCTTCCT GCGCCGAGAG 
GTCCAGGATG TGGTGAAGTG CCAGATGGGG GTGTGCCGGG CTGATGAGAG CGAAGACTCC 
CCTGACTCGT GTAAGAACGG GCAGCTGCAG ATCCTGTCAG ACTTTGAAAA GGATGTGGAT 
CTGGCTTGTC AAACAGTGCT GTTCAAGGAG GTCAACACTT GCAACCCGTC CACCATCACG 
GGCACACTAT CCCGCCTGTC CCTGGATGAG GATGAGGAGC CCAAGTCCTG CCTCGTGGGC 
CCTGAGGGCA GCCTCAGCTT CTCACCACTG CCTGGGAATA TCCTGGTGCC CATGGCAGCC 
TCACCAGGGC TGGGGGAGCC TCCGCCCCCA CAGGAGGCCA ACCCTGTTTA CATGTGTGGG 
GAGGGTGGCC TGCGGCAGCT GGACCTCACA TGGCTGCGGC CCACTGAGCC AGGCTCTGAG 
GGAGACTACA TGGTGCTGCC CCGGCGGACT TTGAGCCTGC AGCCTGGCGG TGGGGGTGGA 
GGTGGTGAGG ATGCCCCCAG GGCCCGGCCG GAGGGGACCC CCCGGCGAGC TGCCAAGACA 
GTGGCCCACA CTGAAGGCTA CCCCAGCTTC CTGTCCGTGG ACCACTCGGG CCTGGGGCTG 
GGCCCTGCCT ATGGATCTCT CCAGAATCCC TATGGAATGA CCTTCCAACC GCCACCGCCG 
ACACCCAGCG CCCGCCAAGT GCCCGAGCCA GGGGAGCGCA GCCGGACCAT GCCTCGCACC 
GTGCCCGGCT CTACCATGAA GATGGGCTCC CTGGAGCGAA AGAAATTACG GTATTCAGAC 
CTGGACTTTG AGAAGGTGAT GCACACCCGG AAACGGCATT CAGAACTCTA CCACGAGCTC 
AACCAGAAGT TCCACACTTT CGACCGCTAC CGCAGCCAGT CCACGGCCAA GAGGGAGAAG 
CGGTGGAGTG TGTCCTCGGG TGGGGCAGCC GAGCGGAGCG TGTGCACCGA TAAGCCCAGC 
CCTGGGGAGC GCCCCAGCTT GTCCCAACAT CGGCGCCATC AGAGCTGGAG CACCTTCAAA 
TCTATGACAC TGGGCTCGCT GCCCCCCAAG CCCCGAGAAC GGCTGACTCT GCACCGGGCA 
GCAGCCTGGG AGCCCACAGA ACCACCGGAT GGTGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857399.1
CDS	424..4917
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001910.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001910.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGCACGGC GTGTGGGAGG AGTGGGGGTC CTGGAGCCTG 
TGCTCCCGCA GCTGCGGGCG GGGGTCCCGG AGCCGGATGC GGACCTGCGT GCCCCCCCAG 
CACGGCGGCA AGGCCTGCGA GGGTCCTGAG CTGCAGACTA AGCTCTGCAG TATGGCTGCC 
TGCCCGGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGG CCTCACTCTG GAGCTCCTGC GTGGTGCTGC CCCTGCTGGC GCTCACCTGG 
ATGTCTGCCG TCCTGGCTAT GACAGACCGC CGTTCCGTCC TCTTCCAGGC CCTCTTTGCT 
GTCTTCAACT CCGCGCAGGG CTTTGTCATC ACTGCTGTGC ACTGCTTCCT GCGCCGAGAG 
GTCCAGGATG TGGTGAAGTG CCAGATGGGG GTGTGCCGGG CTGATGAGAG CGAAGACTCC 
CCTGACTCGT GTAAGAACGG GCAGCTGCAG ATCCTGTCAG ACTTTGAAAA GGATGTGGAT 
CTGGCTTGTC AAACAGTGCT GTTCAAGGAG GTCAACACTT GCAACCCGTC CACCATCACG 
GGCACACTAT CCCGCCTGTC CCTGGATGAG GATGAGGAGC CCAAGTCCTG CCTCGTGGGC 
CCTGAGGGCA GCCTCAGCTT CTCACCACTG CCTGGGAATA TCCTGGTGCC CATGGCAGCC 
TCACCAGGGC TGGGGGAGCC TCCGCCCCCA CAGGAGGCCA ACCCTGTTTA CATGTGTGGG 
GAGGGTGGCC TGCGGCAGCT GGACCTCACA TGGCTGCGGC CCACTGAGCC AGGCTCTGAG 
GGAGACTACA TGGTGCTGCC CCGGCGGACT TTGAGCCTGC AGCCTGGCGG TGGGGGTGGA 
GGTGGTGAGG ATGCCCCCAG GGCCCGGCCG GAGGGGACCC CCCGGCGAGC TGCCAAGACA 
GTGGCCCACA CTGAAGGCTA CCCCAGCTTC CTGTCCGTGG ACCACTCGGG CCTGGGGCTG 
GGCCCTGCCT ATGGATCTCT CCAGAATCCC TATGGAATGA CCTTCCAACC GCCACCGCCG 
ACACCCAGCG CCCGCCAAGT GCCCGAGCCA GGGGAGCGCA GCCGGACCAT GCCTCGCACC 
GTGCCCGGCT CTACCATGAA GATGGGCTCC CTGGAGCGAA AGAAATTACG GTATTCAGAC 
CTGGACTTTG AGAAGGTGAT GCACACCCGG AAACGGCATT CAGAACTCTA CCACGAGCTC 
AACCAGAAGT TCCACACTTT CGACCGCTAC CGCAGCCAGT CCACGGCCAA GAGGGAGAAG 
CGGTGGAGTG TGTCCTCGGG TGGGGCAGCC GAGCGGAGCG TGTGCACCGA TAAGCCCAGC 
CCTGGGGAGC GCCCCAGCTT GTCCCAACAT CGGCGCCATC AGAGCTGGAG CACCTTCAAA 
TCTATGACAC TGGGCTCGCT GCCCCCCAAG CCCCGAGAAC GGCTGACTCT GCACCGGGCA 
GCAGCCTGGG AGCCCACAGA ACCACCGGAT GGTGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001911.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857400.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X16, mRNA.

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CloneID	OHu33647
Clone ID Related Accession (Same CDS sequence)	XM_017001911.1
Accession Version	XM_017001911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGCACGGC GTGTGGGAGG AGTGGGGGTC CTGGAGCCTG 
TGCTCCCGCA GCTGCGGGCG GGGGTCCCGG AGCCGGATGC GGACCTGCGT GCCCCCCCAG 
CACGGCGGCA AGGCCTGCGA GGGTCCTGAG CTGCAGACTA AGCTCTGCAG TATGGCTGCC 
TGCCCGGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGG CCTCACTCTG GAGCTCCTGC GTGGTGCTGC CCCTGCTGGC GCTCACCTGG 
ATGTCTGCCG TCCTGGCTAT GACAGACCGC CGTTCCGTCC TCTTCCAGGC CCTCTTTGCT 
GTCTTCAACT CCGCGCAGGG CTTTGTCATC ACTGCTGTGC ACTGCTTCCT GCGCCGAGAG 
GTCCAGGATG TGGTGAAGTG CCAGATGGGG GTGTGCCGGG CTGATGAGAG CGAAGACTCC 
CCTGACTCGT GTAAGAACGG GCAGCTGCAG ATCCTGTCAG ACTTTGAAAA GGATGTGGAT 
CTGGCTTGTC AAACAGTGCT GTTCAAGGAG GTCAACACTT GCAACCCGTC CACCATCACG 
GGCACACTAT CCCGCCTGTC CCTGGATGAG GATGAGGAGC CCAAGTCCTG CCTCGTGGGC 
CCTGAGGGCA GCCTCAGCTT CTCACCACTG CCTGGGAATA TCCTGGTGCC CATGGCAGCC 
TCACCAGGGC TGGGGGAGCC TCCGCCCCCA CAGGAGGCCA ACCCTGTTTA CATGTGTGGG 
GAGGGTGGCC TGCGGCAGCT GGACCTCACA TGGCTGCGGC CCACTGAGCC AGGCTCTGAG 
GGAGACTACA TGGTGCTGCC CCGGCGGACT TTGAGCCTGC AGCCTGGCGG TGGGGGTGGA 
GGTGGTGAGG ATGCCCCCAG GGCCCGGCCG GAGGGGACCC CCCGGCGAGC TGCCAAGACA 
GTGGCCCACA CTGAAGGCTA CCCCAGCTTC CTGTCCGTGG ACCACTCGGG CCTGGGGCTG 
GGCCCTGCCT ATGGATCTCT CCAGAATCCC TATGGAATGA CCTTCCAACC GCCACCGCCG 
ACACCCAGCG CCCGCCAAGT GCCCGAGCCA GGGGAGCGCA GCCGGACCAT GCCTCGCACC 
GTGCCCGGCT CTACCATGAA GATGGGCTCC CTGGAGCGAA AGAAATTACG GTATTCAGAC 
CTGGACTTTG AGAAGGTGAT GCACACCCGG AAACGGCATT CAGAACTCTA CCACGAGCTC 
AACCAGAAGT TCCACACTTT CGACCGCTAC CGCAGCCAGT CCACGGCCAA GGATAAGCCC 
AGCCCTGGGG AGCGCCCCAG CTTGTCCCAA CATCGGCGCC ATCAGAGCTG GAGCACCTTC 
AAATCTATGA CACTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGAC TCTGCACCGG 
GCAGCAGCCT GGGAGCCCAC AGAACCACCG GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857400.1
CDS	425..4861
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001911.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001911.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGCACGGC GTGTGGGAGG AGTGGGGGTC CTGGAGCCTG 
TGCTCCCGCA GCTGCGGGCG GGGGTCCCGG AGCCGGATGC GGACCTGCGT GCCCCCCCAG 
CACGGCGGCA AGGCCTGCGA GGGTCCTGAG CTGCAGACTA AGCTCTGCAG TATGGCTGCC 
TGCCCGGTGG AAGGCCAGTG GTTAGAATGG GGTCCCTGGG GCCCATGCTC CACGTCCTGT 
GCCAATGGGA CCCAACAGCG CAGCCGGAAG TGCAGCGTGG CGGGCCCAGC CTGGGCCACA 
TGCACGGGTG CCCTCACTGA CACCCGGGAG TGCAGCAACC TCGAGTGCCC GGCCACTGAT 
AGCAAGTGGG GGCCATGGAA TGCGTGGAGC CTGTGCTCTA AGACGTGTGA CACAGGCTGG 
CAGCGCCGCT TCCGCATGTG CCAGGCCACG GGCACGCAGG GCTACCCCTG CGAGGGCACC 
GGAGAGGAGG TGAAGCCTTG TAGTGAGAAG AGGTGTCCAG CCTTCCATGA GATGTGCAGG 
GATGAGTACG TGATGCTGAT GACGTGGAAG AAGGCAGCTG CTGGCGAGAT CATCTACAAC 
AAGTGCCCCC CGAATGCCTC AGGGTCTGCC AGCCGCCGCT GTCTCCTCAG TGCCCAAGGC 
GTGGCGTACT GGGGGCTGCC CAGCTTTGCT CGCTGCATCT CCCATGAGTA CCGCTACCTG 
TATCTGTCAC TTAGGGAGCA CCTGGCCAAG GGGCAGCGCA TGCTGGCAGG CGAGGGCATG 
TCGCAGGTGG TGCGCAGCCT GCAGGAGCTA CTGGCCCGGC GCACCTACTA TAGTGGGGAC 
CTGCTCTTCT CTGTGGACAT TCTGAGGAAT GTCACTGACA CCTTTAAGAG GGCCACCTAC 
GTGCCCTCGG CTGATGATGT GCAGCGCTTC TTCCAGGTGG TGAGCTTCAT GGTGGATGCG 
GAAAACAAGG AGAAGTGGGA CGATGCTCAG CAGGTGTCCC CTGGCTCTGT GCACCTGCTC 
CGTGTCGTGG AGGACTTCAT TCACCTGGTG GGCGATGCTC TCAAGGCCTT CCAGAGCTCT 
CTGATTGTCA CAGATAATCT AGTGATCAGC ATTCAGCGAG AGCCCGTCTC AGCTGTGTCC 
AGTGACATCA CGTTCCCCAT GCGGGGCCGC CGGGGCATGA AGGACTGGGT GCGGCACTCA 
GAGGACCGCC TCTTCCTGCC CAAGGAGGTG CTCAGCCTCT CCTCCCCAGG GAAGCCAGCC 
ACATCTGGGG CAGCAGGCAG CCCTGGCAGG GGGAGGGGCC CAGGAACGGT GCCTCCTGGC 
CCAGGCCACT CCCACCAGCG CCTCCTCCCA GCAGACCCTG ATGAGTCCTC CTACTTTGTG 
ATCGGTGCTG TACTCTACCG CACCCTTGGC CTCATCCTGC CGCCTCCCAG GCCCCCGCTG 
GCCGTCACAT CCCGGGTGAT GACAGTGACT GTGCGCCCCC CTACCCAGCC TCCAGCTGAG 
CCCCTCATCA CTGTGGAGCT CTCCTACATC ATCAATGGGA CCACGGATCC CCATTGCGCC 
AGCTGGGACT ACTCCAGAGC AGATGCCAGC TCAGGAGACT GGGACACTGA AAATTGCCAG 
ACCCTGGAGA CCCAGGCAGC TCACACCCGC TGCCAGTGCC AGCACCTGTC CACCTTTGCT 
GTACTAGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCTCCCC CTCGGTCCCC 
CTGGTGATCG GCTGTGCAGT GTCGTGCATG GCGCTGCTCA CCCTGCTCGC CATCTATGCC 
GCCTTTTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC 
ATCTTGGCAT CCAACATCCT GATCCTCGTG GGCCAGTCCC GGGTGCTGAG CAAGGGCGTG 
TGCACCATGA CGGCTGCCTT CCTGCACTTC TTCTTTCTCT CCTCCTTTTG CTGGGTGCTT 
ACCGAGGCCT GGCAGTCCTA CCTGGCTGTC ATTGGGCGGA TGCGCACCCG CCTCGTTCGC 
AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCTGTTGGC 
TTTACCCGAA CGAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCCCT GGAGGGCGGC 
CTGCTCTACG CCTTTGTGGG CCCTGCAGCC GTCATTGTCC TGGTGAACAT GCTCATCGGA 
ATCATCGTCT TCAACAAGCT CATGGCACGT GATGGCATCT CCGACAAATC CAAGAAGCAG 
AGGGCCGGGG CCTCACTCTG GAGCTCCTGC GTGGTGCTGC CCCTGCTGGC GCTCACCTGG 
ATGTCTGCCG TCCTGGCTAT GACAGACCGC CGTTCCGTCC TCTTCCAGGC CCTCTTTGCT 
GTCTTCAACT CCGCGCAGGG CTTTGTCATC ACTGCTGTGC ACTGCTTCCT GCGCCGAGAG 
GTCCAGGATG TGGTGAAGTG CCAGATGGGG GTGTGCCGGG CTGATGAGAG CGAAGACTCC 
CCTGACTCGT GTAAGAACGG GCAGCTGCAG ATCCTGTCAG ACTTTGAAAA GGATGTGGAT 
CTGGCTTGTC AAACAGTGCT GTTCAAGGAG GTCAACACTT GCAACCCGTC CACCATCACG 
GGCACACTAT CCCGCCTGTC CCTGGATGAG GATGAGGAGC CCAAGTCCTG CCTCGTGGGC 
CCTGAGGGCA GCCTCAGCTT CTCACCACTG CCTGGGAATA TCCTGGTGCC CATGGCAGCC 
TCACCAGGGC TGGGGGAGCC TCCGCCCCCA CAGGAGGCCA ACCCTGTTTA CATGTGTGGG 
GAGGGTGGCC TGCGGCAGCT GGACCTCACA TGGCTGCGGC CCACTGAGCC AGGCTCTGAG 
GGAGACTACA TGGTGCTGCC CCGGCGGACT TTGAGCCTGC AGCCTGGCGG TGGGGGTGGA 
GGTGGTGAGG ATGCCCCCAG GGCCCGGCCG GAGGGGACCC CCCGGCGAGC TGCCAAGACA 
GTGGCCCACA CTGAAGGCTA CCCCAGCTTC CTGTCCGTGG ACCACTCGGG CCTGGGGCTG 
GGCCCTGCCT ATGGATCTCT CCAGAATCCC TATGGAATGA CCTTCCAACC GCCACCGCCG 
ACACCCAGCG CCCGCCAAGT GCCCGAGCCA GGGGAGCGCA GCCGGACCAT GCCTCGCACC 
GTGCCCGGCT CTACCATGAA GATGGGCTCC CTGGAGCGAA AGAAATTACG GTATTCAGAC 
CTGGACTTTG AGAAGGTGAT GCACACCCGG AAACGGCATT CAGAACTCTA CCACGAGCTC 
AACCAGAAGT TCCACACTTT CGACCGCTAC CGCAGCCAGT CCACGGCCAA GGATAAGCCC 
AGCCCTGGGG AGCGCCCCAG CTTGTCCCAA CATCGGCGCC ATCAGAGCTG GAGCACCTTC 
AAATCTATGA CACTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGAC TCTGCACCGG 
GCAGCAGCCT GGGAGCCCAC AGAACCACCG GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_011541848.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540150.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

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CloneID	OHu56448
Clone ID Related Accession (Same CDS sequence)	NM_001364857.1 , XM_011541848.3 , XM_011541848.2 , NM_001364857.2 , XM_017001899.1
Accession Version	XM_011541848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011541848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540150.1
CDS	326..5083
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011541848.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541848.3

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_011541848.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540150.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

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CloneID	OHu56448
Clone ID Related Accession (Same CDS sequence)	NM_001364857.1 , XM_011541848.3 , XM_011541848.2 , NM_001364857.2 , XM_017001899.1
Accession Version	XM_011541848.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540150.1
CDS	325..5082
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011541848.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541848.2

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001899.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857388.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu56448
Clone ID Related Accession (Same CDS sequence)	NM_001364857.1 , XM_011541848.3 , XM_011541848.2 , NM_001364857.2 , XM_017001899.1
Accession Version	XM_017001899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857388.1
CDS	455..5212
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001899.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001899.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGGCGACCCG GCGGCTGAGG AGTGGTCCCC GTGGAGCGTG 
TGTTCCCTGA CGTGTGGGCA GGGTCTGCAG GTGCGGACCC GCTCCTGTGT GTCCTCCCCC 
TATGGGACCC TGTGCAGCGG GCCCCTGCGG GAGACCAGGC CCTGCAACAA TTCAGCCACC 
TGCCCAGTGC ACGGCGTGTG GGAGGAGTGG GGGTCCTGGA GCCTGTGCTC CCGCAGCTGC 
GGGCGGGGGT CCCGGAGCCG GATGCGGACC TGCGTGCCCC CCCAGCACGG CGGCAAGGCC 
TGCGAGGGTC CTGAGCTGCA GACTAAGCTC TGCAGTATGG CTGCCTGCCC GGTGGAAGGC 
CAGTGGTTAG AATGGGGTCC CTGGGGCCCA TGCTCCACGT CCTGTGCCAA TGGGACCCAA 
CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC CCAGCCTGGG CCACATGCAC GGGTGCCCTC 
ACTGACACCC GGGAGTGCAG CAACCTCGAG TGCCCGGCCA CTGATAGCAA GTGGGGGCCA 
TGGAATGCGT GGAGCCTGTG CTCTAAGACG TGTGACACAG GCTGGCAGCG CCGCTTCCGC 
ATGTGCCAGG CCACGGGCAC GCAGGGCTAC CCCTGCGAGG GCACCGGAGA GGAGGTGAAG 
CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC CATGAGATGT GCAGGGATGA GTACGTGATG 
CTGATGACGT GGAAGAAGGC AGCTGCTGGC GAGATCATCT ACAACAAGTG CCCCCCGAAT 
GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC CTCAGTGCCC AAGGCGTGGC GTACTGGGGG 
CTGCCCAGCT TTGCTCGCTG CATCTCCCAT GAGTACCGCT ACCTGTATCT GTCACTTAGG 
GAGCACCTGG CCAAGGGGCA GCGCATGCTG GCAGGCGAGG GCATGTCGCA GGTGGTGCGC 
AGCCTGCAGG AGCTACTGGC CCGGCGCACC TACTATAGTG GGGACCTGCT CTTCTCTGTG 
GACATTCTGA GGAATGTCAC TGACACCTTT AAGAGGGCCA CCTACGTGCC CTCGGCTGAT 
GATGTGCAGC GCTTCTTCCA GGTGGTGAGC TTCATGGTGG ATGCGGAAAA CAAGGAGAAG 
TGGGACGATG CTCAGCAGGT GTCCCCTGGC TCTGTGCACC TGCTCCGTGT CGTGGAGGAC 
TTCATTCACC TGGTGGGCGA TGCTCTCAAG GCCTTCCAGA GCTCTCTGAT TGTCACAGAT 
AATCTAGTGA TCAGCATTCA GCGAGAGCCC GTCTCAGCTG TGTCCAGTGA CATCACGTTC 
CCCATGCGGG GCCGCCGGGG CATGAAGGAC TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC 
CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC CCAGGGAAGC CAGCCACATC TGGGGCAGCA 
GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA ACGGTGCCTC CTGGCCCAGG CCACTCCCAC 
CAGCGCCTCC TCCCAGCAGA CCCTGATGAG TCCTCCTACT TTGTGATCGG TGCTGTACTC 
TACCGCACCC TTGGCCTCAT CCTGCCGCCT CCCAGGCCCC CGCTGGCCGT CACATCCCGG 
GTGATGACAG TGACTGTGCG CCCCCCTACC CAGCCTCCAG CTGAGCCCCT CATCACTGTG 
GAGCTCTCCT ACATCATCAA TGGGACCACG GATCCCCATT GCGCCAGCTG GGACTACTCC 
AGAGCAGATG CCAGCTCAGG AGACTGGGAC ACTGAAAATT GCCAGACCCT GGAGACCCAG 
GCAGCTCACA CCCGCTGCCA GTGCCAGCAC CTGTCCACCT TTGCTGTACT AGCCCAGCCG 
CCCAAGGACC TGACCCTGGA GCTGGCGGGC TCCCCCTCGG TCCCCCTGGT GATCGGCTGT 
GCAGTGTCGT GCATGGCGCT GCTCACCCTG CTCGCCATCT ATGCCGCCTT TTGGAGGTTC 
ATAAAATCTG AACGCTCCAT CATCTTGCTG AACTTCTGCC TGTCCATCTT GGCATCCAAC 
ATCCTGATCC TCGTGGGCCA GTCCCGGGTG CTGAGCAAGG GCGTGTGCAC CATGACGGCT 
GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC TTTTGCTGGG TGCTTACCGA GGCCTGGCAG 
TCCTACCTGG CTGTCATTGG GCGGATGCGC ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC 
CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG GCCGTGTCTG TTGGCTTTAC CCGAACGAAA 
GGATACGGTA CATCCAGCTA CTGCTGGCTC TCCCTGGAGG GCGGCCTGCT CTACGCCTTT 
GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG AACATGCTCA TCGGAATCAT CGTCTTCAAC 
AAGCTCATGG CACGTGATGG CATCTCCGAC AAATCCAAGA AGCAGAGGGC CGGGTCGGAG 
CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC 
CTGCTCAGCT CAGCCTCGGC CAGGAACGCC ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG 
CTGCCCCTGC TGGCGCTCAC CTGGATGTCT GCCGTCCTGG CTATGACAGA CCGCCGTTCC 
GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC AACTCCGCGC AGGGCTTTGT CATCACTGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTGA AGTGCCAGAT GGGGGTGTGC 
CGGGCTGATG AGAGCGAAGA CTCCCCTGAC TCGTGTAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGATGT GGATCTGGCT TGTCAAACAG TGCTGTTCAA GGAGGTCAAC 
ACTTGCAACC CGTCCACCAT CACGGGCACA CTATCCCGCC TGTCCCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG GGCAGCCTCA GCTTCTCACC ACTGCCTGGG 
AATATCCTGG TGCCCATGGC AGCCTCACCA GGGCTGGGGG AGCCTCCGCC CCCACAGGAG 
GCCAACCCTG TTTACATGTG TGGGGAGGGT GGCCTGCGGC AGCTGGACCT CACATGGCTG 
CGGCCCACTG AGCCAGGCTC TGAGGGAGAC TACATGGTGC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT GAGGATGCCC CCAGGGCCCG GCCGGAGGGG 
ACCCCCCGGC GAGCTGCCAA GACAGTGGCC CACACTGAAG GCTACCCCAG CTTCCTGTCC 
GTGGACCACT CGGGCCTGGG GCTGGGCCCT GCCTATGGAT CTCTCCAGAA TCCCTATGGA 
ATGACCTTCC AACCGCCACC GCCGACACCC AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG 
CGCAGCCGGA CCATGCCTCG CACCGTGCCC GGCTCTACCA TGAAGATGGG CTCCCTGGAG 
CGAAAGAAAT TACGGTATTC AGACCTGGAC TTTGAGAAGG TGATGCACAC CCGGAAACGG 
CATTCAGAAC TCTACCACGA GCTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGCAGC 
CAGTCCACGG CCAAGAGGGA GAAGCGGTGG AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG 
AGCGTGTGCA CCGATAAGCC CAGCCCTGGG GAGCGCCCCA GCTTGTCCCA ACATCGGCGC 
CATCAGAGCT GGAGCACCTT CAAATCTATG ACACTGGGCT CGCTGCCCCC CAAGCCCCGA 
GAACGGCTGA CTCTGCACCG GGCAGCAGCC TGGGAGCCCA CAGAACCACC GGATGGTGAC 
TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_011541849.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540151.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu56449
Clone ID Related Accession (Same CDS sequence)	XM_011541849.2 , XM_024448673.1
Accession Version	XM_011541849.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACCGCC 
TTCGACCCCG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GTGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCGCACAC 
TTTGCCCCCC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
AGCCCCGAGG AGGCGGTGGC CCAGGCGGAG TCAGAGGTGG GGCGGCCAGA AGAGGAGGAG 
GCAGAGGCGG CAGCGGGGTT GGAGCTGTGC AGCGGCTCAG GCCCCTTTAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCTGAGC CCTCCGAGGC CCCGCGCCTG 
CTGGCGCCCG CTGCCCTAGC CTTCCGCTTT GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCTAGCCAAT TCACCTGTGG TGTGCTCTGC CGCTGGAGTG AGGAGTGTGG CCGCGCTGCC 
GGCAGGGCCT GCGGCTTTGC TCAGCCAGGC TGCAGCTGCC CTGGAGAGGC GGGGGCCGGC 
TCCACCACCA CCACATCTCC AGGCCCTCCT GCTGCCCACA CCCTGTCCAA TGCCCTGGTG 
CCCGGGGGCC CAGCCCCACC TGCTGAGGCC GATTTGCACT CGGGGAGCAG CAATGATCTG 
TTCACAACCG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAAGT GAAAACCCAG 
TGGCCGAGGT CTGCAGATGA GCCTGGGCTA TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GTGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
AGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCTGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCTGCCT GCCCGGTGGA AGGCCAGTGG TTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACGTCCTGTG CCAATGGGAC CCAACAGCGC AGCCGGAAGT GCAGCGTGGC GGGCCCAGCC 
TGGGCCACAT GCACGGGTGC CCTCACTGAC ACCCGGGAGT GCAGCAACCT CGAGTGCCCG 
GCCACTGATA GCAAGTGGGG GCCATGGAAT GCGTGGAGCC TGTGCTCTAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GCACGCAGGG CTACCCCTGC 
GAGGGCACCG GAGAGGAGGT GAAGCCTTGT AGTGAGAAGA GGTGTCCAGC CTTCCATGAG 
ATGTGCAGGG ATGAGTACGT GATGCTGATG ACGTGGAAGA AGGCAGCTGC TGGCGAGATC 
ATCTACAACA AGTGCCCCCC GAATGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAAGGCG TGGCGTACTG GGGGCTGCCC AGCTTTGCTC GCTGCATCTC CCATGAGTAC 
CGCTACCTGT ATCTGTCACT TAGGGAGCAC CTGGCCAAGG GGCAGCGCAT GCTGGCAGGC 
GAGGGCATGT CGCAGGTGGT GCGCAGCCTG CAGGAGCTAC TGGCCCGGCG CACCTACTAT 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTGAGGAATG TCACTGACAC CTTTAAGAGG 
GCCACCTACG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCGG AAAACAAGGA GAAGTGGGAC GATGCTCAGC AGGTGTCCCC TGGCTCTGTG 
CACCTGCTCC GTGTCGTGGA GGACTTCATT CACCTGGTGG GCGATGCTCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGATAATCTA GTGATCAGCA TTCAGCGAGA GCCCGTCTCA 
GCTGTGTCCA GTGACATCAC GTTCCCCATG CGGGGCCGCC GGGGCATGAA GGACTGGGTG 
CGGCACTCAG AGGACCGCCT CTTCCTGCCC AAGGAGGTGC TCAGCCTCTC CTCCCCAGGG 
AAGCCAGCCA CATCTGGGGC AGCAGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACGGTG 
CCTCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTCCCAG CAGACCCTGA TGAGTCCTCC 
TACTTTGTGA TCGGTGCTGT ACTCTACCGC ACCCTTGGCC TCATCCTGCC GCCTCCCAGG 
CCCCCGCTGG CCGTCACATC CCGGGTGATG ACAGTGACTG TGCGCCCCCC TACCCAGCCT 
CCAGCTGAGC CCCTCATCAC TGTGGAGCTC TCCTACATCA TCAATGGGAC CACGGATCCC 
CATTGCGCCA GCTGGGACTA CTCCAGAGCA GATGCCAGCT CAGGAGACTG GGACACTGAA 
AATTGCCAGA CCCTGGAGAC CCAGGCAGCT CACACCCGCT GCCAGTGCCA GCACCTGTCC 
ACCTTTGCTG TACTAGCCCA GCCGCCCAAG GACCTGACCC TGGAGCTGGC GGGCTCCCCC 
TCGGTCCCCC TGGTGATCGG CTGTGCAGTG TCGTGCATGG CGCTGCTCAC CCTGCTCGCC 
ATCTATGCCG CCTTTTGGAG GTTCATAAAA TCTGAACGCT CCATCATCTT GCTGAACTTC 
TGCCTGTCCA TCTTGGCATC CAACATCCTG ATCCTCGTGG GCCAGTCCCG GGTGCTGAGC 
AAGGGCGTGT GCACCATGAC GGCTGCCTTC CTGCACTTCT TCTTTCTCTC CTCCTTTTGC 
TGGGTGCTTA CCGAGGCCTG GCAGTCCTAC CTGGCTGTCA TTGGGCGGAT GCGCACCCGC 
CTCGTTCGCA AGCGCTTCCT CTGCCTGGGC TGGGGTCTGC CTGCCCTGGT GGTGGCCGTG 
TCTGTTGGCT TTACCCGAAC GAAAGGATAC GGTACATCCA GCTACTGCTG GCTCTCCCTG 
GAGGGCGGCC TGCTCTACGC CTTTGTGGGC CCTGCAGCCG TCATTGTCCT GGTGAACATG 
CTCATCGGAA TCATCGTCTT CAACAAGCTC ATGGCACGTG ATGGCATCTC CGACAAATCC 
AAGAAGCAGA GGGCCGGGTC GGAGCGGTGC CCCTGGGCCA GCCTGCTCCT CCCCTGCTCA 
GCGTGTGGAG CGGTCCCCAG CCCCCTGCTC AGCTCAGCCT CGGCCAGGAA CGCCATGGCC 
TCACTCTGGA GCTCCTGCGT GGTGCTGCCC CTGCTGGCGC TCACCTGGAT GTCTGCCGTC 
CTGGCTATGA CAGACCGCCG TTCCGTCCTC TTCCAGGCCC TCTTTGCTGT CTTCAACTCC 
GCGCAGGGCT TTGTCATCAC TGCTGTGCAC TGCTTCCTGC GCCGAGAGGT CCAGGATGTG 
GTGAAGTGCC AGATGGGGGT GTGCCGGGCT GATGAGAGCG AAGACTCCCC TGACTCGTGT 
AAGAACGGGC AGCTGCAGAT CCTGTCAGAC TTTGAAAAGG ATGTGGATCT GGCTTGTCAA 
ACAGTGCTGT TCAAGGAGGT CAACACTTGC AACCCGTCCA CCATCACGGG CACACTATCC 
CGCCTGTCCC TGGATGAGGA TGAGGAGCCC AAGTCCTGCC TCGTGGGCCC TGAGGGCAGC 
CTCAGCTTCT CACCACTGCC TGGGAATATC CTGGTGCCCA TGGCAGCCTC ACCAGGGCTG 
GGGGAGCCTC CGCCCCCACA GGAGGCCAAC CCTGTTTACA TGTGTGGGGA GGGTGGCCTG 
CGGCAGCTGG ACCTCACATG GCTGCGGCCC ACTGAGCCAG GCTCTGAGGG AGACTACATG 
GTGCTGCCCC GGCGGACTTT GAGCCTGCAG CCTGGCGGTG GGGGTGGAGG TGGTGAGGAT 
GCCCCCAGGG CCCGGCCGGA GGGGACCCCC CGGCGAGCTG CCAAGACAGT GGCCCACACT 
GAAGGCTACC CCAGCTTCCT GTCCGTGGAC CACTCGGGCC TGGGGCTGGG CCCTGCCTAT 
GGATCTCTCC AGAATCCCTA TGGAATGACC TTCCAACCGC CACCGCCGAC ACCCAGCGCC 
CGCCAAGTGC CCGAGCCAGG GGAGCGCAGC CGGACCATGC CTCGCACCGT GCCCGGCTCT 
ACCATGAAGA TGGGCTCCCT GGAGCGAAAG AAATTACGGT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA ACGGCATTCA GAACTCTACC ACGAGCTCAA CCAGAAGTTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCC ACGGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGGCAGCCGA GCGGAGCGTG TGCACCGATA AGCCCAGCCC TGGGGAGCGC 
CCCAGCTTGT CCCAACATCG GCGCCATCAG AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGACTCTGC ACCGGGCAGC AGCCTGGGAG 
CCCACAGAAC CACCGGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540151.1
CDS	196..4917
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011541849.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541849.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACCGCC 
TTCGACCCCG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GTGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCGCACAC 
TTTGCCCCCC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
AGCCCCGAGG AGGCGGTGGC CCAGGCGGAG TCAGAGGTGG GGCGGCCAGA AGAGGAGGAG 
GCAGAGGCGG CAGCGGGGTT GGAGCTGTGC AGCGGCTCAG GCCCCTTTAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCTGAGC CCTCCGAGGC CCCGCGCCTG 
CTGGCGCCCG CTGCCCTAGC CTTCCGCTTT GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCTAGCCAAT TCACCTGTGG TGTGCTCTGC CGCTGGAGTG AGGAGTGTGG CCGCGCTGCC 
GGCAGGGCCT GCGGCTTTGC TCAGCCAGGC TGCAGCTGCC CTGGAGAGGC GGGGGCCGGC 
TCCACCACCA CCACATCTCC AGGCCCTCCT GCTGCCCACA CCCTGTCCAA TGCCCTGGTG 
CCCGGGGGCC CAGCCCCACC TGCTGAGGCC GATTTGCACT CGGGGAGCAG CAATGATCTG 
TTCACAACCG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAAGT GAAAACCCAG 
TGGCCGAGGT CTGCAGATGA GCCTGGGCTA TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GTGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
AGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCTGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCTGCCT GCCCGGTGGA AGGCCAGTGG TTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACGTCCTGTG CCAATGGGAC CCAACAGCGC AGCCGGAAGT GCAGCGTGGC GGGCCCAGCC 
TGGGCCACAT GCACGGGTGC CCTCACTGAC ACCCGGGAGT GCAGCAACCT CGAGTGCCCG 
GCCACTGATA GCAAGTGGGG GCCATGGAAT GCGTGGAGCC TGTGCTCTAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GCACGCAGGG CTACCCCTGC 
GAGGGCACCG GAGAGGAGGT GAAGCCTTGT AGTGAGAAGA GGTGTCCAGC CTTCCATGAG 
ATGTGCAGGG ATGAGTACGT GATGCTGATG ACGTGGAAGA AGGCAGCTGC TGGCGAGATC 
ATCTACAACA AGTGCCCCCC GAATGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAAGGCG TGGCGTACTG GGGGCTGCCC AGCTTTGCTC GCTGCATCTC CCATGAGTAC 
CGCTACCTGT ATCTGTCACT TAGGGAGCAC CTGGCCAAGG GGCAGCGCAT GCTGGCAGGC 
GAGGGCATGT CGCAGGTGGT GCGCAGCCTG CAGGAGCTAC TGGCCCGGCG CACCTACTAT 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTGAGGAATG TCACTGACAC CTTTAAGAGG 
GCCACCTACG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCGG AAAACAAGGA GAAGTGGGAC GATGCTCAGC AGGTGTCCCC TGGCTCTGTG 
CACCTGCTCC GTGTCGTGGA GGACTTCATT CACCTGGTGG GCGATGCTCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGATAATCTA GTGATCAGCA TTCAGCGAGA GCCCGTCTCA 
GCTGTGTCCA GTGACATCAC GTTCCCCATG CGGGGCCGCC GGGGCATGAA GGACTGGGTG 
CGGCACTCAG AGGACCGCCT CTTCCTGCCC AAGGAGGTGC TCAGCCTCTC CTCCCCAGGG 
AAGCCAGCCA CATCTGGGGC AGCAGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACGGTG 
CCTCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTCCCAG CAGACCCTGA TGAGTCCTCC 
TACTTTGTGA TCGGTGCTGT ACTCTACCGC ACCCTTGGCC TCATCCTGCC GCCTCCCAGG 
CCCCCGCTGG CCGTCACATC CCGGGTGATG ACAGTGACTG TGCGCCCCCC TACCCAGCCT 
CCAGCTGAGC CCCTCATCAC TGTGGAGCTC TCCTACATCA TCAATGGGAC CACGGATCCC 
CATTGCGCCA GCTGGGACTA CTCCAGAGCA GATGCCAGCT CAGGAGACTG GGACACTGAA 
AATTGCCAGA CCCTGGAGAC CCAGGCAGCT CACACCCGCT GCCAGTGCCA GCACCTGTCC 
ACCTTTGCTG TACTAGCCCA GCCGCCCAAG GACCTGACCC TGGAGCTGGC GGGCTCCCCC 
TCGGTCCCCC TGGTGATCGG CTGTGCAGTG TCGTGCATGG CGCTGCTCAC CCTGCTCGCC 
ATCTATGCCG CCTTTTGGAG GTTCATAAAA TCTGAACGCT CCATCATCTT GCTGAACTTC 
TGCCTGTCCA TCTTGGCATC CAACATCCTG ATCCTCGTGG GCCAGTCCCG GGTGCTGAGC 
AAGGGCGTGT GCACCATGAC GGCTGCCTTC CTGCACTTCT TCTTTCTCTC CTCCTTTTGC 
TGGGTGCTTA CCGAGGCCTG GCAGTCCTAC CTGGCTGTCA TTGGGCGGAT GCGCACCCGC 
CTCGTTCGCA AGCGCTTCCT CTGCCTGGGC TGGGGTCTGC CTGCCCTGGT GGTGGCCGTG 
TCTGTTGGCT TTACCCGAAC GAAAGGATAC GGTACATCCA GCTACTGCTG GCTCTCCCTG 
GAGGGCGGCC TGCTCTACGC CTTTGTGGGC CCTGCAGCCG TCATTGTCCT GGTGAACATG 
CTCATCGGAA TCATCGTCTT CAACAAGCTC ATGGCACGTG ATGGCATCTC CGACAAATCC 
AAGAAGCAGA GGGCCGGGTC GGAGCGGTGC CCCTGGGCCA GCCTGCTCCT CCCCTGCTCA 
GCGTGTGGAG CGGTCCCCAG CCCCCTGCTC AGCTCAGCCT CGGCCAGGAA CGCCATGGCC 
TCACTCTGGA GCTCCTGCGT GGTGCTGCCC CTGCTGGCGC TCACCTGGAT GTCTGCCGTC 
CTGGCTATGA CAGACCGCCG TTCCGTCCTC TTCCAGGCCC TCTTTGCTGT CTTCAACTCC 
GCGCAGGGCT TTGTCATCAC TGCTGTGCAC TGCTTCCTGC GCCGAGAGGT CCAGGATGTG 
GTGAAGTGCC AGATGGGGGT GTGCCGGGCT GATGAGAGCG AAGACTCCCC TGACTCGTGT 
AAGAACGGGC AGCTGCAGAT CCTGTCAGAC TTTGAAAAGG ATGTGGATCT GGCTTGTCAA 
ACAGTGCTGT TCAAGGAGGT CAACACTTGC AACCCGTCCA CCATCACGGG CACACTATCC 
CGCCTGTCCC TGGATGAGGA TGAGGAGCCC AAGTCCTGCC TCGTGGGCCC TGAGGGCAGC 
CTCAGCTTCT CACCACTGCC TGGGAATATC CTGGTGCCCA TGGCAGCCTC ACCAGGGCTG 
GGGGAGCCTC CGCCCCCACA GGAGGCCAAC CCTGTTTACA TGTGTGGGGA GGGTGGCCTG 
CGGCAGCTGG ACCTCACATG GCTGCGGCCC ACTGAGCCAG GCTCTGAGGG AGACTACATG 
GTGCTGCCCC GGCGGACTTT GAGCCTGCAG CCTGGCGGTG GGGGTGGAGG TGGTGAGGAT 
GCCCCCAGGG CCCGGCCGGA GGGGACCCCC CGGCGAGCTG CCAAGACAGT GGCCCACACT 
GAAGGCTACC CCAGCTTCCT GTCCGTGGAC CACTCGGGCC TGGGGCTGGG CCCTGCCTAT 
GGATCTCTCC AGAATCCCTA TGGAATGACC TTCCAACCGC CACCGCCGAC ACCCAGCGCC 
CGCCAAGTGC CCGAGCCAGG GGAGCGCAGC CGGACCATGC CTCGCACCGT GCCCGGCTCT 
ACCATGAAGA TGGGCTCCCT GGAGCGAAAG AAATTACGGT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA ACGGCATTCA GAACTCTACC ACGAGCTCAA CCAGAAGTTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCC ACGGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGGCAGCCGA GCGGAGCGTG TGCACCGATA AGCCCAGCCC TGGGGAGCGC 
CCCAGCTTGT CCCAACATCG GCGCCATCAG AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGACTCTGC ACCGGGCAGC AGCCTGGGAG 
CCCACAGAAC CACCGGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_024448673.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304441.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.

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CloneID	OHu56449
Clone ID Related Accession (Same CDS sequence)	XM_011541849.2 , XM_024448673.1
Accession Version	XM_024448673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACCGCC 
TTCGACCCCG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GTGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCGCACAC 
TTTGCCCCCC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
AGCCCCGAGG AGGCGGTGGC CCAGGCGGAG TCAGAGGTGG GGCGGCCAGA AGAGGAGGAG 
GCAGAGGCGG CAGCGGGGTT GGAGCTGTGC AGCGGCTCAG GCCCCTTTAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCTGAGC CCTCCGAGGC CCCGCGCCTG 
CTGGCGCCCG CTGCCCTAGC CTTCCGCTTT GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCTAGCCAAT TCACCTGTGG TGTGCTCTGC CGCTGGAGTG AGGAGTGTGG CCGCGCTGCC 
GGCAGGGCCT GCGGCTTTGC TCAGCCAGGC TGCAGCTGCC CTGGAGAGGC GGGGGCCGGC 
TCCACCACCA CCACATCTCC AGGCCCTCCT GCTGCCCACA CCCTGTCCAA TGCCCTGGTG 
CCCGGGGGCC CAGCCCCACC TGCTGAGGCC GATTTGCACT CGGGGAGCAG CAATGATCTG 
TTCACAACCG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAAGT GAAAACCCAG 
TGGCCGAGGT CTGCAGATGA GCCTGGGCTA TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GTGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
AGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCTGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCTGCCT GCCCGGTGGA AGGCCAGTGG TTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACGTCCTGTG CCAATGGGAC CCAACAGCGC AGCCGGAAGT GCAGCGTGGC GGGCCCAGCC 
TGGGCCACAT GCACGGGTGC CCTCACTGAC ACCCGGGAGT GCAGCAACCT CGAGTGCCCG 
GCCACTGATA GCAAGTGGGG GCCATGGAAT GCGTGGAGCC TGTGCTCTAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GCACGCAGGG CTACCCCTGC 
GAGGGCACCG GAGAGGAGGT GAAGCCTTGT AGTGAGAAGA GGTGTCCAGC CTTCCATGAG 
ATGTGCAGGG ATGAGTACGT GATGCTGATG ACGTGGAAGA AGGCAGCTGC TGGCGAGATC 
ATCTACAACA AGTGCCCCCC GAATGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAAGGCG TGGCGTACTG GGGGCTGCCC AGCTTTGCTC GCTGCATCTC CCATGAGTAC 
CGCTACCTGT ATCTGTCACT TAGGGAGCAC CTGGCCAAGG GGCAGCGCAT GCTGGCAGGC 
GAGGGCATGT CGCAGGTGGT GCGCAGCCTG CAGGAGCTAC TGGCCCGGCG CACCTACTAT 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTGAGGAATG TCACTGACAC CTTTAAGAGG 
GCCACCTACG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCGG AAAACAAGGA GAAGTGGGAC GATGCTCAGC AGGTGTCCCC TGGCTCTGTG 
CACCTGCTCC GTGTCGTGGA GGACTTCATT CACCTGGTGG GCGATGCTCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGATAATCTA GTGATCAGCA TTCAGCGAGA GCCCGTCTCA 
GCTGTGTCCA GTGACATCAC GTTCCCCATG CGGGGCCGCC GGGGCATGAA GGACTGGGTG 
CGGCACTCAG AGGACCGCCT CTTCCTGCCC AAGGAGGTGC TCAGCCTCTC CTCCCCAGGG 
AAGCCAGCCA CATCTGGGGC AGCAGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACGGTG 
CCTCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTCCCAG CAGACCCTGA TGAGTCCTCC 
TACTTTGTGA TCGGTGCTGT ACTCTACCGC ACCCTTGGCC TCATCCTGCC GCCTCCCAGG 
CCCCCGCTGG CCGTCACATC CCGGGTGATG ACAGTGACTG TGCGCCCCCC TACCCAGCCT 
CCAGCTGAGC CCCTCATCAC TGTGGAGCTC TCCTACATCA TCAATGGGAC CACGGATCCC 
CATTGCGCCA GCTGGGACTA CTCCAGAGCA GATGCCAGCT CAGGAGACTG GGACACTGAA 
AATTGCCAGA CCCTGGAGAC CCAGGCAGCT CACACCCGCT GCCAGTGCCA GCACCTGTCC 
ACCTTTGCTG TACTAGCCCA GCCGCCCAAG GACCTGACCC TGGAGCTGGC GGGCTCCCCC 
TCGGTCCCCC TGGTGATCGG CTGTGCAGTG TCGTGCATGG CGCTGCTCAC CCTGCTCGCC 
ATCTATGCCG CCTTTTGGAG GTTCATAAAA TCTGAACGCT CCATCATCTT GCTGAACTTC 
TGCCTGTCCA TCTTGGCATC CAACATCCTG ATCCTCGTGG GCCAGTCCCG GGTGCTGAGC 
AAGGGCGTGT GCACCATGAC GGCTGCCTTC CTGCACTTCT TCTTTCTCTC CTCCTTTTGC 
TGGGTGCTTA CCGAGGCCTG GCAGTCCTAC CTGGCTGTCA TTGGGCGGAT GCGCACCCGC 
CTCGTTCGCA AGCGCTTCCT CTGCCTGGGC TGGGGTCTGC CTGCCCTGGT GGTGGCCGTG 
TCTGTTGGCT TTACCCGAAC GAAAGGATAC GGTACATCCA GCTACTGCTG GCTCTCCCTG 
GAGGGCGGCC TGCTCTACGC CTTTGTGGGC CCTGCAGCCG TCATTGTCCT GGTGAACATG 
CTCATCGGAA TCATCGTCTT CAACAAGCTC ATGGCACGTG ATGGCATCTC CGACAAATCC 
AAGAAGCAGA GGGCCGGGTC GGAGCGGTGC CCCTGGGCCA GCCTGCTCCT CCCCTGCTCA 
GCGTGTGGAG CGGTCCCCAG CCCCCTGCTC AGCTCAGCCT CGGCCAGGAA CGCCATGGCC 
TCACTCTGGA GCTCCTGCGT GGTGCTGCCC CTGCTGGCGC TCACCTGGAT GTCTGCCGTC 
CTGGCTATGA CAGACCGCCG TTCCGTCCTC TTCCAGGCCC TCTTTGCTGT CTTCAACTCC 
GCGCAGGGCT TTGTCATCAC TGCTGTGCAC TGCTTCCTGC GCCGAGAGGT CCAGGATGTG 
GTGAAGTGCC AGATGGGGGT GTGCCGGGCT GATGAGAGCG AAGACTCCCC TGACTCGTGT 
AAGAACGGGC AGCTGCAGAT CCTGTCAGAC TTTGAAAAGG ATGTGGATCT GGCTTGTCAA 
ACAGTGCTGT TCAAGGAGGT CAACACTTGC AACCCGTCCA CCATCACGGG CACACTATCC 
CGCCTGTCCC TGGATGAGGA TGAGGAGCCC AAGTCCTGCC TCGTGGGCCC TGAGGGCAGC 
CTCAGCTTCT CACCACTGCC TGGGAATATC CTGGTGCCCA TGGCAGCCTC ACCAGGGCTG 
GGGGAGCCTC CGCCCCCACA GGAGGCCAAC CCTGTTTACA TGTGTGGGGA GGGTGGCCTG 
CGGCAGCTGG ACCTCACATG GCTGCGGCCC ACTGAGCCAG GCTCTGAGGG AGACTACATG 
GTGCTGCCCC GGCGGACTTT GAGCCTGCAG CCTGGCGGTG GGGGTGGAGG TGGTGAGGAT 
GCCCCCAGGG CCCGGCCGGA GGGGACCCCC CGGCGAGCTG CCAAGACAGT GGCCCACACT 
GAAGGCTACC CCAGCTTCCT GTCCGTGGAC CACTCGGGCC TGGGGCTGGG CCCTGCCTAT 
GGATCTCTCC AGAATCCCTA TGGAATGACC TTCCAACCGC CACCGCCGAC ACCCAGCGCC 
CGCCAAGTGC CCGAGCCAGG GGAGCGCAGC CGGACCATGC CTCGCACCGT GCCCGGCTCT 
ACCATGAAGA TGGGCTCCCT GGAGCGAAAG AAATTACGGT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA ACGGCATTCA GAACTCTACC ACGAGCTCAA CCAGAAGTTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCC ACGGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGGCAGCCGA GCGGAGCGTG TGCACCGATA AGCCCAGCCC TGGGGAGCGC 
CCCAGCTTGT CCCAACATCG GCGCCATCAG AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGACTCTGC ACCGGGCAGC AGCCTGGGAG 
CCCACAGAAC CACCGGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304441.1
CDS	453..5174
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_024448673.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448673.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACCGCC 
TTCGACCCCG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GTGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCGCACAC 
TTTGCCCCCC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
AGCCCCGAGG AGGCGGTGGC CCAGGCGGAG TCAGAGGTGG GGCGGCCAGA AGAGGAGGAG 
GCAGAGGCGG CAGCGGGGTT GGAGCTGTGC AGCGGCTCAG GCCCCTTTAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCTGAGC CCTCCGAGGC CCCGCGCCTG 
CTGGCGCCCG CTGCCCTAGC CTTCCGCTTT GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCTAGCCAAT TCACCTGTGG TGTGCTCTGC CGCTGGAGTG AGGAGTGTGG CCGCGCTGCC 
GGCAGGGCCT GCGGCTTTGC TCAGCCAGGC TGCAGCTGCC CTGGAGAGGC GGGGGCCGGC 
TCCACCACCA CCACATCTCC AGGCCCTCCT GCTGCCCACA CCCTGTCCAA TGCCCTGGTG 
CCCGGGGGCC CAGCCCCACC TGCTGAGGCC GATTTGCACT CGGGGAGCAG CAATGATCTG 
TTCACAACCG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAAGT GAAAACCCAG 
TGGCCGAGGT CTGCAGATGA GCCTGGGCTA TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GTGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
AGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCTGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCTGCCT GCCCGGTGGA AGGCCAGTGG TTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACGTCCTGTG CCAATGGGAC CCAACAGCGC AGCCGGAAGT GCAGCGTGGC GGGCCCAGCC 
TGGGCCACAT GCACGGGTGC CCTCACTGAC ACCCGGGAGT GCAGCAACCT CGAGTGCCCG 
GCCACTGATA GCAAGTGGGG GCCATGGAAT GCGTGGAGCC TGTGCTCTAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GCACGCAGGG CTACCCCTGC 
GAGGGCACCG GAGAGGAGGT GAAGCCTTGT AGTGAGAAGA GGTGTCCAGC CTTCCATGAG 
ATGTGCAGGG ATGAGTACGT GATGCTGATG ACGTGGAAGA AGGCAGCTGC TGGCGAGATC 
ATCTACAACA AGTGCCCCCC GAATGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAAGGCG TGGCGTACTG GGGGCTGCCC AGCTTTGCTC GCTGCATCTC CCATGAGTAC 
CGCTACCTGT ATCTGTCACT TAGGGAGCAC CTGGCCAAGG GGCAGCGCAT GCTGGCAGGC 
GAGGGCATGT CGCAGGTGGT GCGCAGCCTG CAGGAGCTAC TGGCCCGGCG CACCTACTAT 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTGAGGAATG TCACTGACAC CTTTAAGAGG 
GCCACCTACG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCGG AAAACAAGGA GAAGTGGGAC GATGCTCAGC AGGTGTCCCC TGGCTCTGTG 
CACCTGCTCC GTGTCGTGGA GGACTTCATT CACCTGGTGG GCGATGCTCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGATAATCTA GTGATCAGCA TTCAGCGAGA GCCCGTCTCA 
GCTGTGTCCA GTGACATCAC GTTCCCCATG CGGGGCCGCC GGGGCATGAA GGACTGGGTG 
CGGCACTCAG AGGACCGCCT CTTCCTGCCC AAGGAGGTGC TCAGCCTCTC CTCCCCAGGG 
AAGCCAGCCA CATCTGGGGC AGCAGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACGGTG 
CCTCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTCCCAG CAGACCCTGA TGAGTCCTCC 
TACTTTGTGA TCGGTGCTGT ACTCTACCGC ACCCTTGGCC TCATCCTGCC GCCTCCCAGG 
CCCCCGCTGG CCGTCACATC CCGGGTGATG ACAGTGACTG TGCGCCCCCC TACCCAGCCT 
CCAGCTGAGC CCCTCATCAC TGTGGAGCTC TCCTACATCA TCAATGGGAC CACGGATCCC 
CATTGCGCCA GCTGGGACTA CTCCAGAGCA GATGCCAGCT CAGGAGACTG GGACACTGAA 
AATTGCCAGA CCCTGGAGAC CCAGGCAGCT CACACCCGCT GCCAGTGCCA GCACCTGTCC 
ACCTTTGCTG TACTAGCCCA GCCGCCCAAG GACCTGACCC TGGAGCTGGC GGGCTCCCCC 
TCGGTCCCCC TGGTGATCGG CTGTGCAGTG TCGTGCATGG CGCTGCTCAC CCTGCTCGCC 
ATCTATGCCG CCTTTTGGAG GTTCATAAAA TCTGAACGCT CCATCATCTT GCTGAACTTC 
TGCCTGTCCA TCTTGGCATC CAACATCCTG ATCCTCGTGG GCCAGTCCCG GGTGCTGAGC 
AAGGGCGTGT GCACCATGAC GGCTGCCTTC CTGCACTTCT TCTTTCTCTC CTCCTTTTGC 
TGGGTGCTTA CCGAGGCCTG GCAGTCCTAC CTGGCTGTCA TTGGGCGGAT GCGCACCCGC 
CTCGTTCGCA AGCGCTTCCT CTGCCTGGGC TGGGGTCTGC CTGCCCTGGT GGTGGCCGTG 
TCTGTTGGCT TTACCCGAAC GAAAGGATAC GGTACATCCA GCTACTGCTG GCTCTCCCTG 
GAGGGCGGCC TGCTCTACGC CTTTGTGGGC CCTGCAGCCG TCATTGTCCT GGTGAACATG 
CTCATCGGAA TCATCGTCTT CAACAAGCTC ATGGCACGTG ATGGCATCTC CGACAAATCC 
AAGAAGCAGA GGGCCGGGTC GGAGCGGTGC CCCTGGGCCA GCCTGCTCCT CCCCTGCTCA 
GCGTGTGGAG CGGTCCCCAG CCCCCTGCTC AGCTCAGCCT CGGCCAGGAA CGCCATGGCC 
TCACTCTGGA GCTCCTGCGT GGTGCTGCCC CTGCTGGCGC TCACCTGGAT GTCTGCCGTC 
CTGGCTATGA CAGACCGCCG TTCCGTCCTC TTCCAGGCCC TCTTTGCTGT CTTCAACTCC 
GCGCAGGGCT TTGTCATCAC TGCTGTGCAC TGCTTCCTGC GCCGAGAGGT CCAGGATGTG 
GTGAAGTGCC AGATGGGGGT GTGCCGGGCT GATGAGAGCG AAGACTCCCC TGACTCGTGT 
AAGAACGGGC AGCTGCAGAT CCTGTCAGAC TTTGAAAAGG ATGTGGATCT GGCTTGTCAA 
ACAGTGCTGT TCAAGGAGGT CAACACTTGC AACCCGTCCA CCATCACGGG CACACTATCC 
CGCCTGTCCC TGGATGAGGA TGAGGAGCCC AAGTCCTGCC TCGTGGGCCC TGAGGGCAGC 
CTCAGCTTCT CACCACTGCC TGGGAATATC CTGGTGCCCA TGGCAGCCTC ACCAGGGCTG 
GGGGAGCCTC CGCCCCCACA GGAGGCCAAC CCTGTTTACA TGTGTGGGGA GGGTGGCCTG 
CGGCAGCTGG ACCTCACATG GCTGCGGCCC ACTGAGCCAG GCTCTGAGGG AGACTACATG 
GTGCTGCCCC GGCGGACTTT GAGCCTGCAG CCTGGCGGTG GGGGTGGAGG TGGTGAGGAT 
GCCCCCAGGG CCCGGCCGGA GGGGACCCCC CGGCGAGCTG CCAAGACAGT GGCCCACACT 
GAAGGCTACC CCAGCTTCCT GTCCGTGGAC CACTCGGGCC TGGGGCTGGG CCCTGCCTAT 
GGATCTCTCC AGAATCCCTA TGGAATGACC TTCCAACCGC CACCGCCGAC ACCCAGCGCC 
CGCCAAGTGC CCGAGCCAGG GGAGCGCAGC CGGACCATGC CTCGCACCGT GCCCGGCTCT 
ACCATGAAGA TGGGCTCCCT GGAGCGAAAG AAATTACGGT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA ACGGCATTCA GAACTCTACC ACGAGCTCAA CCAGAAGTTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCC ACGGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGGCAGCCGA GCGGAGCGTG TGCACCGATA AGCCCAGCCC TGGGGAGCGC 
CCCAGCTTGT CCCAACATCG GCGCCATCAG AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGACTCTGC ACCGGGCAGC AGCCTGGGAG 
CCCACAGAAC CACCGGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_017001912.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857401.1 Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X17, mRNA.

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CloneID	OHu56450
Clone ID Related Accession (Same CDS sequence)	XM_017001912.1
Accession Version	XM_017001912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	adhesion G protein-coupled receptor B2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGGAAGGC CAGTGGTTAG AATGGGGTCC CTGGGGCCCA 
TGCTCCACGT CCTGTGCCAA TGGGACCCAA CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC 
CCAGCCTGGG CCACATGCAC GGGTGCCCTC ACTGACACCC GGGAGTGCAG CAACCTCGAG 
TGCCCGGCCA CTGATAGCAA GTGGGGGCCA TGGAATGCGT GGAGCCTGTG CTCTAAGACG 
TGTGACACAG GCTGGCAGCG CCGCTTCCGC ATGTGCCAGG CCACGGGCAC GCAGGGCTAC 
CCCTGCGAGG GCACCGGAGA GGAGGTGAAG CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC 
CATGAGATGT GCAGGGATGA GTACGTGATG CTGATGACGT GGAAGAAGGC AGCTGCTGGC 
GAGATCATCT ACAACAAGTG CCCCCCGAAT GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC 
CTCAGTGCCC AAGGCGTGGC GTACTGGGGG CTGCCCAGCT TTGCTCGCTG CATCTCCCAT 
GAGTACCGCT ACCTGTATCT GTCACTTAGG GAGCACCTGG CCAAGGGGCA GCGCATGCTG 
GCAGGCGAGG GCATGTCGCA GGTGGTGCGC AGCCTGCAGG AGCTACTGGC CCGGCGCACC 
TACTATAGTG GGGACCTGCT CTTCTCTGTG GACATTCTGA GGAATGTCAC TGACACCTTT 
AAGAGGGCCA CCTACGTGCC CTCGGCTGAT GATGTGCAGC GCTTCTTCCA GGTGGTGAGC 
TTCATGGTGG ATGCGGAAAA CAAGGAGAAG TGGGACGATG CTCAGCAGGT GTCCCCTGGC 
TCTGTGCACC TGCTCCGTGT CGTGGAGGAC TTCATTCACC TGGTGGGCGA TGCTCTCAAG 
GCCTTCCAGA GCTCTCTGAT TGTCACAGAT AATCTAGTGA TCAGCATTCA GCGAGAGCCC 
GTCTCAGCTG TGTCCAGTGA CATCACGTTC CCCATGCGGG GCCGCCGGGG CATGAAGGAC 
TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC 
CCAGGGAAGC CAGCCACATC TGGGGCAGCA GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA 
ACGGTGCCTC CTGGCCCAGG CCACTCCCAC CAGCGCCTCC TCCCAGCAGA CCCTGATGAG 
TCCTCCTACT TTGTGATCGG TGCTGTACTC TACCGCACCC TTGGCCTCAT CCTGCCGCCT 
CCCAGGCCCC CGCTGGCCGT CACATCCCGG GTGATGACAG TGACTGTGCG CCCCCCTACC 
CAGCCTCCAG CTGAGCCCCT CATCACTGTG GAGCTCTCCT ACATCATCAA TGGGACCACG 
GATCCCCATT GCGCCAGCTG GGACTACTCC AGAGCAGATG CCAGCTCAGG AGACTGGGAC 
ACTGAAAATT GCCAGACCCT GGAGACCCAG GCAGCTCACA CCCGCTGCCA GTGCCAGCAC 
CTGTCCACCT TTGCTGTACT AGCCCAGCCG CCCAAGGACC TGACCCTGGA GCTGGCGGGC 
TCCCCCTCGG TCCCCCTGGT GATCGGCTGT GCAGTGTCGT GCATGGCGCT GCTCACCCTG 
CTCGCCATCT ATGCCGCCTT TTGGAGGTTC ATAAAATCTG AACGCTCCAT CATCTTGCTG 
AACTTCTGCC TGTCCATCTT GGCATCCAAC ATCCTGATCC TCGTGGGCCA GTCCCGGGTG 
CTGAGCAAGG GCGTGTGCAC CATGACGGCT GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC 
TTTTGCTGGG TGCTTACCGA GGCCTGGCAG TCCTACCTGG CTGTCATTGG GCGGATGCGC 
ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG 
GCCGTGTCTG TTGGCTTTAC CCGAACGAAA GGATACGGTA CATCCAGCTA CTGCTGGCTC 
TCCCTGGAGG GCGGCCTGCT CTACGCCTTT GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG 
AACATGCTCA TCGGAATCAT CGTCTTCAAC AAGCTCATGG CACGTGATGG CATCTCCGAC 
AAATCCAAGA AGCAGAGGGC CGGGTCGGAG CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC 
TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC CTGCTCAGCT CAGCCTCGGC CAGGAACGCC 
ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG CTGCCCCTGC TGGCGCTCAC CTGGATGTCT 
GCCGTCCTGG CTATGACAGA CCGCCGTTCC GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC 
AACTCCGCGC AGGGCTTTGT CATCACTGCT GTGCACTGCT TCCTGCGCCG AGAGGTCCAG 
GATGTGGTGA AGTGCCAGAT GGGGGTGTGC CGGGCTGATG AGAGCGAAGA CTCCCCTGAC 
TCGTGTAAGA ACGGGCAGCT GCAGATCCTG TCAGACTTTG AAAAGGATGT GGATCTGGCT 
TGTCAAACAG TGCTGTTCAA GGAGGTCAAC ACTTGCAACC CGTCCACCAT CACGGGCACA 
CTATCCCGCC TGTCCCTGGA TGAGGATGAG GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG 
GGCAGCCTCA GCTTCTCACC ACTGCCTGGG AATATCCTGG TGCCCATGGC AGCCTCACCA 
GGGCTGGGGG AGCCTCCGCC CCCACAGGAG GCCAACCCTG TTTACATGTG TGGGGAGGGT 
GGCCTGCGGC AGCTGGACCT CACATGGCTG CGGCCCACTG AGCCAGGCTC TGAGGGAGAC 
TACATGGTGC TGCCCCGGCG GACTTTGAGC CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT 
GAGGATGCCC CCAGGGCCCG GCCGGAGGGG ACCCCCCGGC GAGCTGCCAA GACAGTGGCC 
CACACTGAAG GCTACCCCAG CTTCCTGTCC GTGGACCACT CGGGCCTGGG GCTGGGCCCT 
GCCTATGGAT CTCTCCAGAA TCCCTATGGA ATGACCTTCC AACCGCCACC GCCGACACCC 
AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG CGCAGCCGGA CCATGCCTCG CACCGTGCCC 
GGCTCTACCA TGAAGATGGG CTCCCTGGAG CGAAAGAAAT TACGGTATTC AGACCTGGAC 
TTTGAGAAGG TGATGCACAC CCGGAAACGG CATTCAGAAC TCTACCACGA GCTCAACCAG 
AAGTTCCACA CTTTCGACCG CTACCGCAGC CAGTCCACGG CCAAGAGGGA GAAGCGGTGG 
AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG AGCGTGTGCA CCGATAAGCC CAGCCCTGGG 
GAGCGCCCCA GCTTGTCCCA ACATCGGCGC CATCAGAGCT GGAGCACCTT CAAATCTATG 
ACACTGGGCT CGCTGCCCCC CAAGCCCCGA GAACGGCTGA CTCTGCACCG GGCAGCAGCC 
TGGGAGCCCA CAGAACCACC GGATGGTGAC TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857401.1
CDS	423..4850
Translation	MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_017001912.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001912.1

ORF Insert Sequence:

ATGGAGAATA CAGGTTGGAT GGGCAAGGGA CATAGGATGA CCCCAGCCTG TCCCCTCTTA 
CTGTCTGTGA TTCTGTCCCT GCGCCTGGCC ACCGCCTTCG ACCCCGCCCC CAGTGCCTGC 
TCTGCCCTGG CCTCGGGTGT GCTCTACGGG GCCTTCTCGC TGCAGGACCT CTTTCCTACC 
ATCGCCTCGG GCTGCTCCTG GACCCTGGAG AACCCTGACC CCACCAAGTA CTCCCTCTAC 
CTGCGCTTCA ACCGCCAGGA GCAGGTGTGC GCACACTTTG CCCCCCGCCT GCTGCCCCTG 
GACCACTACC TGGTCAACTT TACCTGCCTG CGGCCTAGCC CCGAGGAGGC GGTGGCCCAG 
GCGGAGTCAG AGGTGGGGCG GCCAGAAGAG GAGGAGGCAG AGGCGGCAGC GGGGTTGGAG 
CTGTGCAGCG GCTCAGGCCC CTTTACCTTC CTGCACTTCG ACAAGAACTT CGTGCAGCTG 
TGCCTGTCGG CTGAGCCCTC CGAGGCCCCG CGCCTGCTGG CGCCCGCTGC CCTAGCCTTC 
CGCTTTGTCG AGGTCTTGCT CATCAACAAC AACAACTCTA GCCAATTCAC CTGTGGTGTG 
CTCTGCCGCT GGAGTGAGGA GTGTGGCCGC GCTGCCGGCA GGGCCTGCGG CTTTGCTCAG 
CCAGGCTGCA GCTGCCCTGG AGAGGCGGGG GCCGGCTCCA CCACCACCAC ATCTCCAGGC 
CCTCCTGCTG CCCACACCCT GTCCAATGCC CTGGTGCCCG GGGGCCCAGC CCCACCTGCT 
GAGGCCGATT TGCACTCGGG GAGCAGCAAT GATCTGTTCA CAACCGAGAT GAGATATGGT 
GAGGAGCCGG AAGAGGAACC GAAAGTGAAA ACCCAGTGGC CGAGGTCTGC AGATGAGCCT 
GGGCTATACA TGGCGCAGAC AGTGGAAGGC CAGTGGTTAG AATGGGGTCC CTGGGGCCCA 
TGCTCCACGT CCTGTGCCAA TGGGACCCAA CAGCGCAGCC GGAAGTGCAG CGTGGCGGGC 
CCAGCCTGGG CCACATGCAC GGGTGCCCTC ACTGACACCC GGGAGTGCAG CAACCTCGAG 
TGCCCGGCCA CTGATAGCAA GTGGGGGCCA TGGAATGCGT GGAGCCTGTG CTCTAAGACG 
TGTGACACAG GCTGGCAGCG CCGCTTCCGC ATGTGCCAGG CCACGGGCAC GCAGGGCTAC 
CCCTGCGAGG GCACCGGAGA GGAGGTGAAG CCTTGTAGTG AGAAGAGGTG TCCAGCCTTC 
CATGAGATGT GCAGGGATGA GTACGTGATG CTGATGACGT GGAAGAAGGC AGCTGCTGGC 
GAGATCATCT ACAACAAGTG CCCCCCGAAT GCCTCAGGGT CTGCCAGCCG CCGCTGTCTC 
CTCAGTGCCC AAGGCGTGGC GTACTGGGGG CTGCCCAGCT TTGCTCGCTG CATCTCCCAT 
GAGTACCGCT ACCTGTATCT GTCACTTAGG GAGCACCTGG CCAAGGGGCA GCGCATGCTG 
GCAGGCGAGG GCATGTCGCA GGTGGTGCGC AGCCTGCAGG AGCTACTGGC CCGGCGCACC 
TACTATAGTG GGGACCTGCT CTTCTCTGTG GACATTCTGA GGAATGTCAC TGACACCTTT 
AAGAGGGCCA CCTACGTGCC CTCGGCTGAT GATGTGCAGC GCTTCTTCCA GGTGGTGAGC 
TTCATGGTGG ATGCGGAAAA CAAGGAGAAG TGGGACGATG CTCAGCAGGT GTCCCCTGGC 
TCTGTGCACC TGCTCCGTGT CGTGGAGGAC TTCATTCACC TGGTGGGCGA TGCTCTCAAG 
GCCTTCCAGA GCTCTCTGAT TGTCACAGAT AATCTAGTGA TCAGCATTCA GCGAGAGCCC 
GTCTCAGCTG TGTCCAGTGA CATCACGTTC CCCATGCGGG GCCGCCGGGG CATGAAGGAC 
TGGGTGCGGC ACTCAGAGGA CCGCCTCTTC CTGCCCAAGG AGGTGCTCAG CCTCTCCTCC 
CCAGGGAAGC CAGCCACATC TGGGGCAGCA GGCAGCCCTG GCAGGGGGAG GGGCCCAGGA 
ACGGTGCCTC CTGGCCCAGG CCACTCCCAC CAGCGCCTCC TCCCAGCAGA CCCTGATGAG 
TCCTCCTACT TTGTGATCGG TGCTGTACTC TACCGCACCC TTGGCCTCAT CCTGCCGCCT 
CCCAGGCCCC CGCTGGCCGT CACATCCCGG GTGATGACAG TGACTGTGCG CCCCCCTACC 
CAGCCTCCAG CTGAGCCCCT CATCACTGTG GAGCTCTCCT ACATCATCAA TGGGACCACG 
GATCCCCATT GCGCCAGCTG GGACTACTCC AGAGCAGATG CCAGCTCAGG AGACTGGGAC 
ACTGAAAATT GCCAGACCCT GGAGACCCAG GCAGCTCACA CCCGCTGCCA GTGCCAGCAC 
CTGTCCACCT TTGCTGTACT AGCCCAGCCG CCCAAGGACC TGACCCTGGA GCTGGCGGGC 
TCCCCCTCGG TCCCCCTGGT GATCGGCTGT GCAGTGTCGT GCATGGCGCT GCTCACCCTG 
CTCGCCATCT ATGCCGCCTT TTGGAGGTTC ATAAAATCTG AACGCTCCAT CATCTTGCTG 
AACTTCTGCC TGTCCATCTT GGCATCCAAC ATCCTGATCC TCGTGGGCCA GTCCCGGGTG 
CTGAGCAAGG GCGTGTGCAC CATGACGGCT GCCTTCCTGC ACTTCTTCTT TCTCTCCTCC 
TTTTGCTGGG TGCTTACCGA GGCCTGGCAG TCCTACCTGG CTGTCATTGG GCGGATGCGC 
ACCCGCCTCG TTCGCAAGCG CTTCCTCTGC CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG 
GCCGTGTCTG TTGGCTTTAC CCGAACGAAA GGATACGGTA CATCCAGCTA CTGCTGGCTC 
TCCCTGGAGG GCGGCCTGCT CTACGCCTTT GTGGGCCCTG CAGCCGTCAT TGTCCTGGTG 
AACATGCTCA TCGGAATCAT CGTCTTCAAC AAGCTCATGG CACGTGATGG CATCTCCGAC 
AAATCCAAGA AGCAGAGGGC CGGGTCGGAG CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC 
TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC CTGCTCAGCT CAGCCTCGGC CAGGAACGCC 
ATGGCCTCAC TCTGGAGCTC CTGCGTGGTG CTGCCCCTGC TGGCGCTCAC CTGGATGTCT 
GCCGTCCTGG CTATGACAGA CCGCCGTTCC GTCCTCTTCC AGGCCCTCTT TGCTGTCTTC 
AACTCCGCGC AGGGCTTTGT CATCACTGCT GTGCACTGCT TCCTGCGCCG AGAGGTCCAG 
GATGTGGTGA AGTGCCAGAT GGGGGTGTGC CGGGCTGATG AGAGCGAAGA CTCCCCTGAC 
TCGTGTAAGA ACGGGCAGCT GCAGATCCTG TCAGACTTTG AAAAGGATGT GGATCTGGCT 
TGTCAAACAG TGCTGTTCAA GGAGGTCAAC ACTTGCAACC CGTCCACCAT CACGGGCACA 
CTATCCCGCC TGTCCCTGGA TGAGGATGAG GAGCCCAAGT CCTGCCTCGT GGGCCCTGAG 
GGCAGCCTCA GCTTCTCACC ACTGCCTGGG AATATCCTGG TGCCCATGGC AGCCTCACCA 
GGGCTGGGGG AGCCTCCGCC CCCACAGGAG GCCAACCCTG TTTACATGTG TGGGGAGGGT 
GGCCTGCGGC AGCTGGACCT CACATGGCTG CGGCCCACTG AGCCAGGCTC TGAGGGAGAC 
TACATGGTGC TGCCCCGGCG GACTTTGAGC CTGCAGCCTG GCGGTGGGGG TGGAGGTGGT 
GAGGATGCCC CCAGGGCCCG GCCGGAGGGG ACCCCCCGGC GAGCTGCCAA GACAGTGGCC 
CACACTGAAG GCTACCCCAG CTTCCTGTCC GTGGACCACT CGGGCCTGGG GCTGGGCCCT 
GCCTATGGAT CTCTCCAGAA TCCCTATGGA ATGACCTTCC AACCGCCACC GCCGACACCC 
AGCGCCCGCC AAGTGCCCGA GCCAGGGGAG CGCAGCCGGA CCATGCCTCG CACCGTGCCC 
GGCTCTACCA TGAAGATGGG CTCCCTGGAG CGAAAGAAAT TACGGTATTC AGACCTGGAC 
TTTGAGAAGG TGATGCACAC CCGGAAACGG CATTCAGAAC TCTACCACGA GCTCAACCAG 
AAGTTCCACA CTTTCGACCG CTACCGCAGC CAGTCCACGG CCAAGAGGGA GAAGCGGTGG 
AGTGTGTCCT CGGGTGGGGC AGCCGAGCGG AGCGTGTGCA CCGATAAGCC CAGCCCTGGG 
GAGCGCCCCA GCTTGTCCCA ACATCGGCGC CATCAGAGCT GGAGCACCTT CAAATCTATG 
ACACTGGGCT CGCTGCCCCC CAAGCCCCGA GAACGGCTGA CTCTGCACCG GGCAGCAGCC 
TGGGAGCCCA CAGAACCACC GGATGGTGAC TTCCAGACAG AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A disease-associated mutation in the adhesion GPCR BAI2 (ADGRB2) increases receptor signaling activity.
Human mutation38(12)1751-1760(2017 Dec)
Purcell RH,Toro C,Gahl WA,Hall RA

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Pharmacological reviews67(2)338-67(2015)
Hamann J,Aust G,Ara? D,Engel FB,Formstone C,Fredriksson R,Hall RA,Harty BL,Kirchhoff C,Knapp B,Krishnan A,Liebscher I,Lin HH,Martinelli DC,Monk KR,Peeters MC,Piao X,Pr?mel S,Sch?neberg T,Schwartz TW,Singer K,Stacey M,Ushkaryov YA,Vallon M,Wolfrum U,Wright MW,Xu L,Langenhan T,Schi?th HB

Identification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting protein.
Biochemical and biophysical research communications411(4)792-7(2011 Aug)
Zencir S,Ovee M,Dobson MJ,Banerjee M,Topcu Z,Mohanty S

Brain-specific angiogenesis inhibitor 2 (BAI2) may be activated by proteolytic processing.
Journal of receptor and signal transduction research30(3)143-53(2010 Jun)
Okajima D,Kudo G,Yokota H

Brain-specific angiogenesis inhibitor 2 regulates VEGF through GABP that acts as a transcriptional repressor.
FEBS letters580(2)669-76(2006 Jan)
Jeong BC,Kim MY,Lee JH,Kee HJ,Kho DH,Han KE,Qian YR,Kim JK,Kim KK

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ADGRB2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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