TRMT1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRMT1
Entrez Gene ID	55621
Full Name	tRNA methyltransferase 1
Synonyms	TRM1
General protein information	Preferred Names tRNA methyltransferase 1 Names tRNA (guanine(26)-N(2))-dimethyltransferase N(2),N(2)-dimethylguanosine tRNA methyltransferase TRM1 tRNA methyltransferase 1 homolog tRNA 2,2-dimethylguanosine-26 methyltransferase tRNA methyltransferase 1 homolog tRNA(guanine-26,N(2)-N(2)) methyltransferase tRNA(m(2,2)G26)dimethyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.13
Summary	This gene encodes a tRNA-modifying enzyme that acts as a dimethyltransferase, modifying a single guanine residue at position 26 of the tRNA. The encoded enzyme has both mono- and dimethylase activity when exogenously expressed, and uses S-adenosyl methionine as a methyl donor. The C-terminal region of the encoded protein has both a zinc finger motif, and an arginine/proline-rich region. Mutations in this gene have been implicated in autosomal recessive intellectual disorder (ARID). Alternative splicing results in multiple transcript variants encoding different isoforms. There is a pseudogene of this gene on the X chromosome. [provided by RefSeq, May 2017].
Disorder MIM:	611669

mRNA and Protein(s)

mRNA	Protein	Name
XM_005259983.1	XP_005260040.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
XM_011528125.1	XP_011526427.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X4
NM_017722.3	NP_060192.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
NM_001351761.1	NP_001338690.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 3
NM_001351760.1	NP_001338689.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
XM_024451588.1	XP_024307356.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
XM_024451591.1	XP_024307359.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X3
XM_024451592.1	XP_024307360.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X3
NM_001142554.3	NP_001136026.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
NM_001351761.2	NP_001338690.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 3
NM_017722.4	NP_060192.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
NM_001351762.1	NP_001338691.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 4
NM_001136035.4	NP_001129507.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
NM_001136035.2	NP_001129507.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
XM_017026944.1	XP_016882433.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
XM_017026947.1	XP_016882436.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X6
NM_001142554.2	NP_001136026.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
NM_001351760.2	NP_001338689.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
NM_001351762.2	NP_001338691.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 4
XM_024451587.1	XP_024307355.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
XM_024451590.1	XP_024307358.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2
XM_024451593.1	XP_024307361.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X3
NM_001142554.1	NP_001136026.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
XM_011528124.1	XP_011526426.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2
NM_001136035.3	NP_001129507.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
XM_024451589.1	XP_024307357.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
XM_017026945.1	XP_016882434.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X3
XM_006722793.2	XP_006722856.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X4
XM_017026946.1	XP_016882435.1	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X5

Pathways from BioSystems

Homologies

	TRMT1	XP_002808218.1
Mus musculus (house mouse)	Trmt1	NP_932137.2
Caenorhabditis elegans (roundworm)	trm-1	NP_506513.1
Xenopus tropicalis (tropical clawed frog)	trmt1	XP_004919202.1
Danio rerio (zebrafish)	trmt1	XP_685886.4
Kluyveromyces lactis	KLLA0F19404g	XP_455951.1
Neurospora crassa	NCU06336	XP_962370.1
Oryza sativa (rice)	Os03g0786700	NP_001051491.1
Rattus norvegicus (Norway rat)	Trmt1	NP_001013892.1
Drosophila melanogaster (fruit fly)	CG6388	NP_609566.1
Saccharomyces cerevisiae (baker's yeast)	TRM1	NP_010405.3
Schizosaccharomyces pombe (fission yeast)	trm1	NP_596547.1
Magnaporthe oryzae (rice blast fungus)	MGG_01448	XP_003714401.1
Homo sapiens (human)	TRMT1	NP_060192.1
Bos taurus (cattle)	TRMT1	NP_001193303.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009740	XP_318825.4
Arabidopsis thaliana (thale cress)	AT5G15810	NP_197085.2
Pan troglodytes (chimpanzee)	TRMT1	XP_512420.1
Canis lupus familiaris (dog)	TRMT1	XP_533897.5
Eremothecium gossypii	AGOS_AGR075W	NP_986740.1
Arabidopsis thaliana (thale cress)	AT3G02320	NP_186881.2

Gene Ontology
Bibliography

Related articles in PubMed

TRMT1-Catalyzed tRNA Modifications Are Required for Redox Homeostasis To Ensure Proper Cellular Proliferation and Oxidative Stress Survival.
Dewe JM, Fuller BL, Lentini JM, Kellner SM, Fu D
Molecular and cellular biology37(21)(2017 Nov)

The Role of a Novel TRMT1 Gene Mutation and Rare GRM1 Gene Defect in Intellectual Disability in Two Azeri Families.
Davarniya B, Hu H, Kahrizi K, Musante L, Fattahi Z, Hosseini M, Maqsoud F, Farajollahi R, Wienker TF, Ropers HH, Najmabadi H
PloS one10(8)e0129631(2015)

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

The human tRNA(m(2)(2)G(26))dimethyltransferase: functional expression and characterization of a cloned hTRM1 gene.
Liu J, Str?by KB
Nucleic acids research28(18)3445-51(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results uncover a biological role for TRMT1-catalyzed tRNA modification in redox metabolism and show that individuals with TRMT1-associated ID are likely to have major perturbations in cellular homeostasis due to the lack of m2,2G modifications.
Title: TRMT1-Catalyzed tRNA Modifications Are Required for Redox Homeostasis To Ensure Proper Cellular Proliferation and Oxidative Stress Survival.

a missense variant was found in GRM1 gene, while in the second family, a frameshift alteration was identified in TRMT1, both of which were found to co-segregate with the intellectual disability.
Title: The Role of a Novel TRMT1 Gene Mutation and Rare GRM1 Gene Defect in Intellectual Disability in Two Azeri Families.

The following TRMT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRMT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02892	NM_017722.4 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02892	NM_001136035.4 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02892	XM_017026944.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu07550	NM_001351760.2 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu07550	NM_001142554.3 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu51694	NM_001351762.2 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu69540	NM_001351761.2 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu07550	XM_017026945.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu07550	XM_005259983.1 Latest version!	Homo sapiens tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu51693	XM_011528125.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu51693	XM_024451589.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu51693	XM_024451588.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu51693	XM_006722793.2 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu51693	XM_024451587.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu51694	XM_024451592.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu51694	XM_017026947.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu51694	XM_024451591.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu51694	XM_024451593.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu69540	XM_011528124.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu88548	XM_024451590.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu88548	XM_017026946.1 Latest version!	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu69540	NM_001351761.1	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu02892	NM_017722.3	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02892	NM_001136035.2	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu07550	NM_001142554.2	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu07550	NM_001351760.1	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu51694	NM_001351762.1	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu07550	NM_001142554.1	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OHu02892	NM_001136035.3	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRMT1 ( NM_017722.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060192.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 1, mRNA.

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CloneID	OHu02892
Clone ID Related Accession (Same CDS sequence)	NM_017722.3 , NM_017722.4 , NM_001136035.4 , NM_001136035.2 , XM_017026944.1 , NM_001136035.3
Accession Version	NM_017722.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-04
Organism	Homo sapiens(Human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC344734.1, BC040126.1 and BF798026.1. On Dec 10, 2008 this sequence version replaced NM_017722.2. Transcript Variant: This variant (1) is the longest transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803616.258250.1, BC040126.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060192.1
CDS	251..2230
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_017722.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017722.3

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_017722.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060192.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02892
Clone ID Related Accession (Same CDS sequence)	NM_017722.3 , NM_017722.4 , NM_001136035.4 , NM_001136035.2 , XM_017026944.1 , NM_001136035.3
Accession Version	NM_017722.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC313999.1, BC040126.1 and BF798026.1. On May 27, 2017 this sequence version replaced NM_017722.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.258250.1, BC040126.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060192.1
CDS	259..2238
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_017722.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017722.4

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001136035.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129507.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 2, mRNA.

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CloneID	OHu02892
Clone ID Related Accession (Same CDS sequence)	NM_017722.3 , NM_017722.4 , NM_001136035.4 , NM_001136035.2 , XM_017026944.1 , NM_001136035.3
Accession Version	NM_001136035.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005546.1. On Oct 4, 2019 this sequence version replaced NM_001136035.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK000251.1, AK292377.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129507.1
CDS	121..2100
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001136035.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136035.4

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001136035.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129507.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02892
Clone ID Related Accession (Same CDS sequence)	NM_017722.3 , NM_017722.4 , NM_001136035.4 , NM_001136035.2 , XM_017026944.1 , NM_001136035.3
Accession Version	NM_001136035.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-04
Organism	Homo sapiens(Human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK292377.1, AK000251.1 and BF798026.1. On Dec 10, 2008 this sequence version replaced NM_001136035.1. Transcript Variant: This variant (2) has an alternate 5' UTR, as compared to variant 1. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK292377.1, AK000251.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129507.1
CDS	130..2109
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001136035.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136035.2

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001136035.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129507.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 2, mRNA.

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CloneID	OHu02892
Clone ID Related Accession (Same CDS sequence)	NM_017722.3 , NM_017722.4 , NM_001136035.4 , NM_001136035.2 , XM_017026944.1 , NM_001136035.3
Accession Version	NM_001136035.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC364503.1, AK000251.1 and BF798026.1. This sequence is a reference standard in the RefSeqGene project. On May 27, 2017 this sequence version replaced NM_001136035.2. Transcript Variant: This variant (2) has an alternate 5' UTR, as compared to variant 1. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK292377.1, AK000251.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129507.1
CDS	152..2131
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001136035.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136035.3

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_017026944.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882433.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.

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CloneID	OHu02892
Clone ID Related Accession (Same CDS sequence)	NM_017722.3 , NM_017722.4 , NM_001136035.4 , NM_001136035.2 , XM_017026944.1 , NM_001136035.3
Accession Version	XM_017026944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882433.1
CDS	573..2552
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_017026944.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026944.1

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGC 
AAGCAGGCGC TGGTGTTCCA GTGTGTGGGC TGCGGGGCCT TCCACCTTCA GCGTCTCGGC 
AAAGCGTCAG GAGTCCCCAG CGGCCGGGCC AAGTTCTCTG CAGCCTGTGG TCCCCCTGTG 
ACCCCCGAGT GTGAACACTG TGGGCAACGA CACCAGCTTG GTGGCCCCAT GTGGGCAGAG 
CCCATCCATG ACCTGGATTT TGTGGGCCGT GTCCTGGAGG CTGTGAGCGC TAACCCCGGC 
CGCTTCCACA CCTCGGAGCG GATCCGAGGG GTCCTGAGCG TCATCACTGA GGAGCTCCCG 
GACGTGCCTC TGTACTACAC CCTGGACCAG CTGAGCAGCA CCATCCACTG CAACACACCA 
AGCCTCCTGC AGTTGCGGTC GGCCCTCCTC CACGCTGACT TCCGGGTCTC ACTCTCCCAC 
GCCTGTAAGA ACGCTGTGAA GACGGATGCC CCTGCCTCTG CCCTCTGGGA CATCATGCGT 
TGCTGGGAGA AGGAATGTCC GGTGAAACGG GAGCGACTAT CAGAGACTAG CCCAGCGTTC 
CGCATTCTCA GTGTGGAGCC CAGGCTGCAG GCCAACTTCA CCATCCGGGA AGATGCCAAC 
CCCAGCTCCC GACAGCGAGG ACTCAAGCGC TTCCAGGCTA ACCCGGAGGC CAACTGGGGT 
CCCCGGCCTC GTGCCCGGCC AGGGGGCAAG GCGGCCGACG AAGCTATGGA GGAGAGACGC 
AGGCTGCTTC AGAACAAGCG GAAGGAGCCG CCGGAAGATG TGGCCCAGCG GGCTGCCCGG 
CTCAAGACAT TTCCTTGCAA GAGGTTTAAG GAGGGCACCT GTCAACGCGG GGACCAGTGC 
TGCTACTCCC ACAGCCCCCC GACACCCAGG GTTTCTGCTG ATGCTGCCCC TGACTGTCCA 
GAGACCTCCA ACCAGACCCC CCCTGGACCT GGGGCTGCCG CTGGGCCAGG CATAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001351760.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338689.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 4, mRNA.

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CloneID	OHu07550
Clone ID Related Accession (Same CDS sequence)	XM_005259983.1 , NM_001351760.1 , NM_001142554.3 , NM_001142554.2 , NM_001351760.2 , NM_001142554.1 , XM_017026945.1
Accession Version	NM_001351760.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-10
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC364503.1, DC365548.1, AK000251.1, BQ212049.1 and BF798026.1. Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Variants 3 and 4 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: SRR1163658.537464.1, SRR1163655.312361.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338689.1
CDS	152..2044
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASARAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001351760.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351760.1

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001142554.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136026.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07550
Clone ID Related Accession (Same CDS sequence)	XM_005259983.1 , NM_001351760.1 , NM_001142554.3 , NM_001142554.2 , NM_001351760.2 , NM_001142554.1 , XM_017026945.1
Accession Version	NM_001142554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005546.1, DC313999.1 and BC018302.1. On Jun 2, 2019 this sequence version replaced NM_001142554.2. ##Evidence-Data-START## Transcript exon combination :: BC018302.1, SRR1803612.115965.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136026.1
CDS	342..2234
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASARAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001142554.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142554.3

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001142554.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136026.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07550
Clone ID Related Accession (Same CDS sequence)	XM_005259983.1 , NM_001351760.1 , NM_001142554.3 , NM_001142554.2 , NM_001351760.2 , NM_001142554.1 , XM_017026945.1
Accession Version	NM_001142554.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-10
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC313999.1, BC018302.1 and BF798026.1. On May 27, 2017 this sequence version replaced NM_001142554.1. Transcript Variant: This variant (3) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Variants 3 and 4 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: BC018302.1, SRR1803612.115965.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136026.1
CDS	259..2151
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASARAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001142554.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142554.2

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001351760.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338689.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 4, mRNA.

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CloneID	OHu07550
Clone ID Related Accession (Same CDS sequence)	XM_005259983.1 , NM_001351760.1 , NM_001142554.3 , NM_001142554.2 , NM_001351760.2 , NM_001142554.1 , XM_017026945.1
Accession Version	NM_001351760.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC365548.1, AK000251.1 and BQ212049.1. On Jun 2, 2019 this sequence version replaced NM_001351760.1. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.145785.1, SRR1163658.537464.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338689.1
CDS	121..2013
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASARAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001351760.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351760.2

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001142554.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136026.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07550
Clone ID Related Accession (Same CDS sequence)	XM_005259983.1 , NM_001351760.1 , NM_001142554.3 , NM_001142554.2 , NM_001351760.2 , NM_001142554.1 , XM_017026945.1
Accession Version	NM_001142554.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-04
Organism	Homo sapiens(Human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 2
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC344734.1, BC018302.1 and BF798026.1. Transcript Variant: This variant (3) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC018302.1, SRR1803612.115965.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136026.1
CDS	251..2143
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASARAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001142554.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142554.1

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001351762.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338691.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu51694
Clone ID Related Accession (Same CDS sequence)	XM_024451591.1 , XM_024451592.1 , NM_001351762.1 , XM_017026947.1 , NM_001351762.2 , XM_024451593.1
Accession Version	NM_001351762.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-10
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC364503.1, DA202329.1, AK000251.1 and BF798026.1. Transcript Variant: This variant (6) contains an alternate segment in the 5' region, and uses an alternate splice site in the 5' region, resulting in a distinct 5' UTR and use of a downstream start codon, compared to variant 1. Use of the 5'-most initiation codon, as used in variant 1, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning results in translation initiation at the downstream start codon to encode an isoform (4) that has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.269750.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338691.1
CDS	1015..2211
Translation	MAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001351762.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351762.1

ORF Insert Sequence:

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001351762.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338691.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu51694
Clone ID Related Accession (Same CDS sequence)	XM_024451591.1 , XM_024451592.1 , NM_001351762.1 , XM_017026947.1 , NM_001351762.2 , XM_024451593.1
Accession Version	NM_001351762.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA202329.1 and AK000251.1. On Jun 2, 2019 this sequence version replaced NM_001351762.1. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.269750.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338691.1
CDS	984..2180
Translation	MAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001351762.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351762.2

ORF Insert Sequence:

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001351761.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338690.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu69540
Clone ID Related Accession (Same CDS sequence)	NM_001351761.1 , NM_001351761.2 , XM_011528124.1
Accession Version	NM_001351761.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-10
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC364503.1, DC373552.1, AK000251.1 and BF798026.1. On May 27, 2017 this sequence version replaced XM_011528124.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.181469.1, SRR1803612.228964.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAACG GCACCGGGCC CTACGGAGAA GAACGTCCAC GTGAAGTCCA GGAGACGACA 
GTCACCGAGG GGGCTGCCAA AATCGCCTTT CCCAGTGCCA ACGAGGTCTT TTATAACCCG 
GTGCAGGAAT TCAATCGGGA CCTGACATGT GCTGTGATCA CCGAGTTTGC TCGCATTCAG 
CTTGGGGCCA AAGGAATCCA GATCAAGGTT CCAGGAGAGA AGGACACGCA AAAAGTGGTC 
GTGGACTTGT CAGAGCAAGA GGAGGAAAAG GTTGAACTGA AAGAGAGTGA AAACCTGGCC 
TCAGGAGACC AACCTCGCAC AGCGGCCGTG GGGGAGATCT GTGAGGAAGG CCTGCATGTG 
CTGGAAGGCC TGGCAGCTTC AGGCCTACGT TCCATTCGAT TTGCCCTAGA GGTGCCTGGG 
CTCAGATCTG TGGTTGCAAA CGATGCCTCC ACCCGGGCTG TGGATCTCAT ACGCCGGAAT 
GTCCAGCTCA ATGACGTGGC CCACCTGGTA CAGCCGAGCC AAGCAGATGC CCGGATGCTG 
ATGTACCAGC ACCAGAGGGT GTCGGAGAGG TTTGACGTCA TCGATCTGGA CCCCTATGGC 
AGCCCAGCCA CCTTCCTGGA TGCAGCTGTG CAGGCTGTGA GTGAAGGAGG GTTGCTGTGT 
GTGACCTGCA CAGACATGGC GGTGTTGGCG GGGAACAGCG GGGAGACGTG CTACAGCAAG 
TACGGGGCCA TGGCCCTCAA GAGCCGGGCC TGCCACGAGA TGGCCCTGAG AATCGTCCTG 
CACAGCCTGG ACCTCCGCGC CAACTGCTAC CAGCGCTTCG TGGTGCCGCT GCTCAGCATC 
AGCGCTGACT TCTACGTGCG TGTTTTTGTC CGTGTCTTCA CCGGCCAGGC CAAGGTCAAG 
GCCTCAGCCA GCAAGCAGGC GCTGGTGTTC CAGTGTGTGG GCTGCGGGGC CTTCCACCTT 
CAGCGTCTCG GCAAAGCGTC AGGAGTCCCC AGCGGCCGGG CCAAGTTCTC TGCAGCCTGT 
GGTCCCCCTG TGACCCCCGA GTGTGAACAC TGTGGGCAAC GACACCAGCT TGGTGGCCCC 
ATGTGGGCAG AGCCCATCCA TGACCTGGAT TTTGTGGGCC GTGTCCTGGA GGCTGTGAGC 
GCTAACCCCG GCCGCTTCCA CACCTCGGAG CGGATCCGAG GGGTCCTGAG CGTCATCACT 
GAGGAGCTCC CGGACGTGCC TCTGTACTAC ACCCTGGACC AGCTGAGCAG CACCATCCAC 
TGCAACACAC CAAGCCTCCT GCAGTTGCGG TCGGCCCTCC TCCACGCTGA CTTCCGGGTC 
TCACTCTCCC ACGCCTGTAA GAACGCTGTG AAGACGGATG CCCCTGCCTC TGCCCTCTGG 
GACATCATGC GTTGCTGGGA GAAGGAATGT CCGGTGAAAC GGGAGCGACT ATCAGAGACT 
AGCCCAGCGT TCCGCATTCT CAGTGTGGAG CCCAGGCTGC AGGCCAACTT CACCATCCGG 
GAAGATGCCA ACCCCAGCTC CCGACAGCGA GGACTCAAGC GCTTCCAGGC TAACCCGGAG 
GCCAACTGGG GTCCCCGGCC TCGTGCCCGG CCAGGGGGCA AGGCGGCCGA CGAAGCTATG 
GAGGAGAGAC GCAGGCTGCT TCAGAACAAG CGGAAGGAGC CGCCGGAAGA TGTGGCCCAG 
CGGGCTGCCC GGCTCAAGAC ATTTCCTTGC AAGAGGTTTA AGGAGGGCAC CTGTCAACGC 
GGGGACCAGT GCTGCTACTC CCACAGCCCC CCGACACCCA GGGTTTCTGC TGATGCTGCC 
CCTGACTGTC CAGAGACCTC CAACCAGACC CCCCCTGGAC CTGGGGCTGC CGCTGGGCCA 
GGCATAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338690.1
CDS	169..2040
Translation	MENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001351761.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351761.1

ORF Insert Sequence:

ATGGAGAACG GCACCGGGCC CTACGGAGAA GAACGTCCAC GTGAAGTCCA GGAGACGACA 
GTCACCGAGG GGGCTGCCAA AATCGCCTTT CCCAGTGCCA ACGAGGTCTT TTATAACCCG 
GTGCAGGAAT TCAATCGGGA CCTGACATGT GCTGTGATCA CCGAGTTTGC TCGCATTCAG 
CTTGGGGCCA AAGGAATCCA GATCAAGGTT CCAGGAGAGA AGGACACGCA AAAAGTGGTC 
GTGGACTTGT CAGAGCAAGA GGAGGAAAAG GTTGAACTGA AAGAGAGTGA AAACCTGGCC 
TCAGGAGACC AACCTCGCAC AGCGGCCGTG GGGGAGATCT GTGAGGAAGG CCTGCATGTG 
CTGGAAGGCC TGGCAGCTTC AGGCCTACGT TCCATTCGAT TTGCCCTAGA GGTGCCTGGG 
CTCAGATCTG TGGTTGCAAA CGATGCCTCC ACCCGGGCTG TGGATCTCAT ACGCCGGAAT 
GTCCAGCTCA ATGACGTGGC CCACCTGGTA CAGCCGAGCC AAGCAGATGC CCGGATGCTG 
ATGTACCAGC ACCAGAGGGT GTCGGAGAGG TTTGACGTCA TCGATCTGGA CCCCTATGGC 
AGCCCAGCCA CCTTCCTGGA TGCAGCTGTG CAGGCTGTGA GTGAAGGAGG GTTGCTGTGT 
GTGACCTGCA CAGACATGGC GGTGTTGGCG GGGAACAGCG GGGAGACGTG CTACAGCAAG 
TACGGGGCCA TGGCCCTCAA GAGCCGGGCC TGCCACGAGA TGGCCCTGAG AATCGTCCTG 
CACAGCCTGG ACCTCCGCGC CAACTGCTAC CAGCGCTTCG TGGTGCCGCT GCTCAGCATC 
AGCGCTGACT TCTACGTGCG TGTTTTTGTC CGTGTCTTCA CCGGCCAGGC CAAGGTCAAG 
GCCTCAGCCA GCAAGCAGGC GCTGGTGTTC CAGTGTGTGG GCTGCGGGGC CTTCCACCTT 
CAGCGTCTCG GCAAAGCGTC AGGAGTCCCC AGCGGCCGGG CCAAGTTCTC TGCAGCCTGT 
GGTCCCCCTG TGACCCCCGA GTGTGAACAC TGTGGGCAAC GACACCAGCT TGGTGGCCCC 
ATGTGGGCAG AGCCCATCCA TGACCTGGAT TTTGTGGGCC GTGTCCTGGA GGCTGTGAGC 
GCTAACCCCG GCCGCTTCCA CACCTCGGAG CGGATCCGAG GGGTCCTGAG CGTCATCACT 
GAGGAGCTCC CGGACGTGCC TCTGTACTAC ACCCTGGACC AGCTGAGCAG CACCATCCAC 
TGCAACACAC CAAGCCTCCT GCAGTTGCGG TCGGCCCTCC TCCACGCTGA CTTCCGGGTC 
TCACTCTCCC ACGCCTGTAA GAACGCTGTG AAGACGGATG CCCCTGCCTC TGCCCTCTGG 
GACATCATGC GTTGCTGGGA GAAGGAATGT CCGGTGAAAC GGGAGCGACT ATCAGAGACT 
AGCCCAGCGT TCCGCATTCT CAGTGTGGAG CCCAGGCTGC AGGCCAACTT CACCATCCGG 
GAAGATGCCA ACCCCAGCTC CCGACAGCGA GGACTCAAGC GCTTCCAGGC TAACCCGGAG 
GCCAACTGGG GTCCCCGGCC TCGTGCCCGG CCAGGGGGCA AGGCGGCCGA CGAAGCTATG 
GAGGAGAGAC GCAGGCTGCT TCAGAACAAG CGGAAGGAGC CGCCGGAAGA TGTGGCCCAG 
CGGGCTGCCC GGCTCAAGAC ATTTCCTTGC AAGAGGTTTA AGGAGGGCAC CTGTCAACGC 
GGGGACCAGT GCTGCTACTC CCACAGCCCC CCGACACCCA GGGTTTCTGC TGATGCTGCC 
CCTGACTGTC CAGAGACCTC CAACCAGACC CCCCCTGGAC CTGGGGCTGC CGCTGGGCCA 
GGCATAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( NM_001351761.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338690.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu69540
Clone ID Related Accession (Same CDS sequence)	NM_001351761.1 , NM_001351761.2 , XM_011528124.1
Accession Version	NM_001351761.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC373552.1 and AK000251.1. On Jun 2, 2019 this sequence version replaced NM_001351761.1. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.181469.1, SRR1803612.228964.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAACG GCACCGGGCC CTACGGAGAA GAACGTCCAC GTGAAGTCCA GGAGACGACA 
GTCACCGAGG GGGCTGCCAA AATCGCCTTT CCCAGTGCCA ACGAGGTCTT TTATAACCCG 
GTGCAGGAAT TCAATCGGGA CCTGACATGT GCTGTGATCA CCGAGTTTGC TCGCATTCAG 
CTTGGGGCCA AAGGAATCCA GATCAAGGTT CCAGGAGAGA AGGACACGCA AAAAGTGGTC 
GTGGACTTGT CAGAGCAAGA GGAGGAAAAG GTTGAACTGA AAGAGAGTGA AAACCTGGCC 
TCAGGAGACC AACCTCGCAC AGCGGCCGTG GGGGAGATCT GTGAGGAAGG CCTGCATGTG 
CTGGAAGGCC TGGCAGCTTC AGGCCTACGT TCCATTCGAT TTGCCCTAGA GGTGCCTGGG 
CTCAGATCTG TGGTTGCAAA CGATGCCTCC ACCCGGGCTG TGGATCTCAT ACGCCGGAAT 
GTCCAGCTCA ATGACGTGGC CCACCTGGTA CAGCCGAGCC AAGCAGATGC CCGGATGCTG 
ATGTACCAGC ACCAGAGGGT GTCGGAGAGG TTTGACGTCA TCGATCTGGA CCCCTATGGC 
AGCCCAGCCA CCTTCCTGGA TGCAGCTGTG CAGGCTGTGA GTGAAGGAGG GTTGCTGTGT 
GTGACCTGCA CAGACATGGC GGTGTTGGCG GGGAACAGCG GGGAGACGTG CTACAGCAAG 
TACGGGGCCA TGGCCCTCAA GAGCCGGGCC TGCCACGAGA TGGCCCTGAG AATCGTCCTG 
CACAGCCTGG ACCTCCGCGC CAACTGCTAC CAGCGCTTCG TGGTGCCGCT GCTCAGCATC 
AGCGCTGACT TCTACGTGCG TGTTTTTGTC CGTGTCTTCA CCGGCCAGGC CAAGGTCAAG 
GCCTCAGCCA GCAAGCAGGC GCTGGTGTTC CAGTGTGTGG GCTGCGGGGC CTTCCACCTT 
CAGCGTCTCG GCAAAGCGTC AGGAGTCCCC AGCGGCCGGG CCAAGTTCTC TGCAGCCTGT 
GGTCCCCCTG TGACCCCCGA GTGTGAACAC TGTGGGCAAC GACACCAGCT TGGTGGCCCC 
ATGTGGGCAG AGCCCATCCA TGACCTGGAT TTTGTGGGCC GTGTCCTGGA GGCTGTGAGC 
GCTAACCCCG GCCGCTTCCA CACCTCGGAG CGGATCCGAG GGGTCCTGAG CGTCATCACT 
GAGGAGCTCC CGGACGTGCC TCTGTACTAC ACCCTGGACC AGCTGAGCAG CACCATCCAC 
TGCAACACAC CAAGCCTCCT GCAGTTGCGG TCGGCCCTCC TCCACGCTGA CTTCCGGGTC 
TCACTCTCCC ACGCCTGTAA GAACGCTGTG AAGACGGATG CCCCTGCCTC TGCCCTCTGG 
GACATCATGC GTTGCTGGGA GAAGGAATGT CCGGTGAAAC GGGAGCGACT ATCAGAGACT 
AGCCCAGCGT TCCGCATTCT CAGTGTGGAG CCCAGGCTGC AGGCCAACTT CACCATCCGG 
GAAGATGCCA ACCCCAGCTC CCGACAGCGA GGACTCAAGC GCTTCCAGGC TAACCCGGAG 
GCCAACTGGG GTCCCCGGCC TCGTGCCCGG CCAGGGGGCA AGGCGGCCGA CGAAGCTATG 
GAGGAGAGAC GCAGGCTGCT TCAGAACAAG CGGAAGGAGC CGCCGGAAGA TGTGGCCCAG 
CGGGCTGCCC GGCTCAAGAC ATTTCCTTGC AAGAGGTTTA AGGAGGGCAC CTGTCAACGC 
GGGGACCAGT GCTGCTACTC CCACAGCCCC CCGACACCCA GGGTTTCTGC TGATGCTGCC 
CCTGACTGTC CAGAGACCTC CAACCAGACC CCCCCTGGAC CTGGGGCTGC CGCTGGGCCA 
GGCATAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338690.1
CDS	138..2009
Translation	MENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	NM_001351761.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351761.2

ORF Insert Sequence:

ATGGAGAACG GCACCGGGCC CTACGGAGAA GAACGTCCAC GTGAAGTCCA GGAGACGACA 
GTCACCGAGG GGGCTGCCAA AATCGCCTTT CCCAGTGCCA ACGAGGTCTT TTATAACCCG 
GTGCAGGAAT TCAATCGGGA CCTGACATGT GCTGTGATCA CCGAGTTTGC TCGCATTCAG 
CTTGGGGCCA AAGGAATCCA GATCAAGGTT CCAGGAGAGA AGGACACGCA AAAAGTGGTC 
GTGGACTTGT CAGAGCAAGA GGAGGAAAAG GTTGAACTGA AAGAGAGTGA AAACCTGGCC 
TCAGGAGACC AACCTCGCAC AGCGGCCGTG GGGGAGATCT GTGAGGAAGG CCTGCATGTG 
CTGGAAGGCC TGGCAGCTTC AGGCCTACGT TCCATTCGAT TTGCCCTAGA GGTGCCTGGG 
CTCAGATCTG TGGTTGCAAA CGATGCCTCC ACCCGGGCTG TGGATCTCAT ACGCCGGAAT 
GTCCAGCTCA ATGACGTGGC CCACCTGGTA CAGCCGAGCC AAGCAGATGC CCGGATGCTG 
ATGTACCAGC ACCAGAGGGT GTCGGAGAGG TTTGACGTCA TCGATCTGGA CCCCTATGGC 
AGCCCAGCCA CCTTCCTGGA TGCAGCTGTG CAGGCTGTGA GTGAAGGAGG GTTGCTGTGT 
GTGACCTGCA CAGACATGGC GGTGTTGGCG GGGAACAGCG GGGAGACGTG CTACAGCAAG 
TACGGGGCCA TGGCCCTCAA GAGCCGGGCC TGCCACGAGA TGGCCCTGAG AATCGTCCTG 
CACAGCCTGG ACCTCCGCGC CAACTGCTAC CAGCGCTTCG TGGTGCCGCT GCTCAGCATC 
AGCGCTGACT TCTACGTGCG TGTTTTTGTC CGTGTCTTCA CCGGCCAGGC CAAGGTCAAG 
GCCTCAGCCA GCAAGCAGGC GCTGGTGTTC CAGTGTGTGG GCTGCGGGGC CTTCCACCTT 
CAGCGTCTCG GCAAAGCGTC AGGAGTCCCC AGCGGCCGGG CCAAGTTCTC TGCAGCCTGT 
GGTCCCCCTG TGACCCCCGA GTGTGAACAC TGTGGGCAAC GACACCAGCT TGGTGGCCCC 
ATGTGGGCAG AGCCCATCCA TGACCTGGAT TTTGTGGGCC GTGTCCTGGA GGCTGTGAGC 
GCTAACCCCG GCCGCTTCCA CACCTCGGAG CGGATCCGAG GGGTCCTGAG CGTCATCACT 
GAGGAGCTCC CGGACGTGCC TCTGTACTAC ACCCTGGACC AGCTGAGCAG CACCATCCAC 
TGCAACACAC CAAGCCTCCT GCAGTTGCGG TCGGCCCTCC TCCACGCTGA CTTCCGGGTC 
TCACTCTCCC ACGCCTGTAA GAACGCTGTG AAGACGGATG CCCCTGCCTC TGCCCTCTGG 
GACATCATGC GTTGCTGGGA GAAGGAATGT CCGGTGAAAC GGGAGCGACT ATCAGAGACT 
AGCCCAGCGT TCCGCATTCT CAGTGTGGAG CCCAGGCTGC AGGCCAACTT CACCATCCGG 
GAAGATGCCA ACCCCAGCTC CCGACAGCGA GGACTCAAGC GCTTCCAGGC TAACCCGGAG 
GCCAACTGGG GTCCCCGGCC TCGTGCCCGG CCAGGGGGCA AGGCGGCCGA CGAAGCTATG 
GAGGAGAGAC GCAGGCTGCT TCAGAACAAG CGGAAGGAGC CGCCGGAAGA TGTGGCCCAG 
CGGGCTGCCC GGCTCAAGAC ATTTCCTTGC AAGAGGTTTA AGGAGGGCAC CTGTCAACGC 
GGGGACCAGT GCTGCTACTC CCACAGCCCC CCGACACCCA GGGTTTCTGC TGATGCTGCC 
CCTGACTGTC CAGAGACCTC CAACCAGACC CCCCCTGGAC CTGGGGCTGC CGCTGGGCCA 
GGCATAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_005259983.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260040.1 Homo sapiens tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu07550
Clone ID Related Accession (Same CDS sequence)	XM_005259983.1 , NM_001351760.1 , NM_001142554.3 , NM_001142554.2 , NM_001351760.2 , NM_001142554.1 , XM_017026945.1
Accession Version	XM_005259983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
Comment

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005260040.1

Translation

MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASARAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_005259983.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259983.1

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_017026945.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882434.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu07550
Clone ID Related Accession (Same CDS sequence)	XM_005259983.1 , NM_001351760.1 , NM_001142554.3 , NM_001142554.2 , NM_001351760.2 , NM_001142554.1 , XM_017026945.1
Accession Version	XM_017026945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882434.1
CDS	573..2465
Translation	MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASARAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_017026945.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026945.1

ORF Insert Sequence:

ATGCAAGGAT CGTCTCTGTG GCTAAGCCTC ACTTTCCGCT CCGCCCGGGT GCTCTCTAGA 
GCCCGGTTTT TCGAGTGGCA GTCTCCAGGG CTGCCGAATA CAGCAGCGAT GGAGAACGGC 
ACCGGGCCCT ACGGAGAAGA ACGTCCACGT GAAGTCCAGG AGACGACAGT CACCGAGGGG 
GCTGCCAAAA TCGCCTTTCC CAGTGCCAAC GAGGTCTTTT ATAACCCGGT GCAGGAATTC 
AATCGGGACC TGACATGTGC TGTGATCACC GAGTTTGCTC GCATTCAGCT TGGGGCCAAA 
GGAATCCAGA TCAAGGTTCC AGGAGAGAAG GACACGCAAA AAGTGGTCGT GGACTTGTCA 
GAGCAAGAGG AGGAAAAGGT TGAACTGAAA GAGAGTGAAA ACCTGGCCTC AGGAGACCAA 
CCTCGCACAG CGGCCGTGGG GGAGATCTGT GAGGAAGGCC TGCATGTGCT GGAAGGCCTG 
GCAGCTTCAG GCCTACGTTC CATTCGATTT GCCCTAGAGG TGCCTGGGCT CAGATCTGTG 
GTTGCAAACG ATGCCTCCAC CCGGGCTGTG GATCTCATAC GCCGGAATGT CCAGCTCAAT 
GACGTGGCCC ACCTGGTACA GCCGAGCCAA GCAGATGCCC GGATGCTGAT GTACCAGCAC 
CAGAGGGTGT CGGAGAGGTT TGACGTCATC GATCTGGACC CCTATGGCAG CCCAGCCACC 
TTCCTGGATG CAGCTGTGCA GGCTGTGAGT GAAGGAGGGT TGCTGTGTGT GACCTGCACA 
GACATGGCGG TGTTGGCGGG GAACAGCGGG GAGACGTGCT ACAGCAAGTA CGGGGCCATG 
GCCCTCAAGA GCCGGGCCTG CCACGAGATG GCCCTGAGAA TCGTCCTGCA CAGCCTGGAC 
CTCCGCGCCA ACTGCTACCA GCGCTTCGTG GTGCCGCTGC TCAGCATCAG CGCTGACTTC 
TACGTGCGTG TTTTTGTCCG TGTCTTCACC GGCCAGGCCA AGGTCAAGGC CTCAGCCAGG 
GCCAAGTTCT CTGCAGCCTG TGGTCCCCCT GTGACCCCCG AGTGTGAACA CTGTGGGCAA 
CGACACCAGC TTGGTGGCCC CATGTGGGCA GAGCCCATCC ATGACCTGGA TTTTGTGGGC 
CGTGTCCTGG AGGCTGTGAG CGCTAACCCC GGCCGCTTCC ACACCTCGGA GCGGATCCGA 
GGGGTCCTGA GCGTCATCAC TGAGGAGCTC CCGGACGTGC CTCTGTACTA CACCCTGGAC 
CAGCTGAGCA GCACCATCCA CTGCAACACA CCAAGCCTCC TGCAGTTGCG GTCGGCCCTC 
CTCCACGCTG ACTTCCGGGT CTCACTCTCC CACGCCTGTA AGAACGCTGT GAAGACGGAT 
GCCCCTGCCT CTGCCCTCTG GGACATCATG CGTTGCTGGG AGAAGGAATG TCCGGTGAAA 
CGGGAGCGAC TATCAGAGAC TAGCCCAGCG TTCCGCATTC TCAGTGTGGA GCCCAGGCTG 
CAGGCCAACT TCACCATCCG GGAAGATGCC AACCCCAGCT CCCGACAGCG AGGACTCAAG 
CGCTTCCAGG CTAACCCGGA GGCCAACTGG GGTCCCCGGC CTCGTGCCCG GCCAGGGGGC 
AAGGCGGCCG ACGAAGCTAT GGAGGAGAGA CGCAGGCTGC TTCAGAACAA GCGGAAGGAG 
CCGCCGGAAG ATGTGGCCCA GCGGGCTGCC CGGCTCAAGA CATTTCCTTG CAAGAGGTTT 
AAGGAGGGCA CCTGTCAACG CGGGGACCAG TGCTGCTACT CCCACAGCCC CCCGACACCC 
AGGGTTTCTG CTGATGCTGC CCCTGACTGT CCAGAGACCT CCAACCAGAC CCCCCCTGGA 
CCTGGGGCTG CCGCTGGGCC AGGCATAGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_011528125.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526427.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu51693
Clone ID Related Accession (Same CDS sequence)	XM_011528125.1 , XM_024451588.1 , XM_024451587.1 , XM_024451589.1 , XM_006722793.2
Accession Version	XM_011528125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526427.1
CDS	1224..2561
Translation	MLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_011528125.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528125.1

ORF Insert Sequence:

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_024451588.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307356.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu51693
Clone ID Related Accession (Same CDS sequence)	XM_011528125.1 , XM_024451588.1 , XM_024451587.1 , XM_024451589.1 , XM_006722793.2
Accession Version	XM_024451588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307356.1
CDS	1535..2872
Translation	MLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_024451588.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451588.1

ORF Insert Sequence:

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_024451587.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307355.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu51693
Clone ID Related Accession (Same CDS sequence)	XM_011528125.1 , XM_024451588.1 , XM_024451587.1 , XM_024451589.1 , XM_006722793.2
Accession Version	XM_024451587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307355.1
CDS	769..2106
Translation	MLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_024451587.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451587.1

ORF Insert Sequence:

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_024451589.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307357.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu51693
Clone ID Related Accession (Same CDS sequence)	XM_011528125.1 , XM_024451588.1 , XM_024451587.1 , XM_024451589.1 , XM_006722793.2
Accession Version	XM_024451589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307357.1
CDS	560..1897
Translation	MLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_024451589.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451589.1

ORF Insert Sequence:

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_006722793.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006722856.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu51693
Clone ID Related Accession (Same CDS sequence)	XM_011528125.1 , XM_024451588.1 , XM_024451587.1 , XM_024451589.1 , XM_006722793.2
Accession Version	XM_006722793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006722793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006722856.1
CDS	1015..2352
Translation	MLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_006722793.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006722793.2

ORF Insert Sequence:

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGCAA GCAGGCGCTG GTGTTCCAGT GTGTGGGCTG CGGGGCCTTC 
CACCTTCAGC GTCTCGGCAA AGCGTCAGGA GTCCCCAGCG GCCGGGCCAA GTTCTCTGCA 
GCCTGTGGTC CCCCTGTGAC CCCCGAGTGT GAACACTGTG GGCAACGACA CCAGCTTGGT 
GGCCCCATGT GGGCAGAGCC CATCCATGAC CTGGATTTTG TGGGCCGTGT CCTGGAGGCT 
GTGAGCGCTA ACCCCGGCCG CTTCCACACC TCGGAGCGGA TCCGAGGGGT CCTGAGCGTC 
ATCACTGAGG AGCTCCCGGA CGTGCCTCTG TACTACACCC TGGACCAGCT GAGCAGCACC 
ATCCACTGCA ACACACCAAG CCTCCTGCAG TTGCGGTCGG CCCTCCTCCA CGCTGACTTC 
CGGGTCTCAC TCTCCCACGC CTGTAAGAAC GCTGTGAAGA CGGATGCCCC TGCCTCTGCC 
CTCTGGGACA TCATGCGTTG CTGGGAGAAG GAATGTCCGG TGAAACGGGA GCGACTATCA 
GAGACTAGCC CAGCGTTCCG CATTCTCAGT GTGGAGCCCA GGCTGCAGGC CAACTTCACC 
ATCCGGGAAG ATGCCAACCC CAGCTCCCGA CAGCGAGGAC TCAAGCGCTT CCAGGCTAAC 
CCGGAGGCCA ACTGGGGTCC CCGGCCTCGT GCCCGGCCAG GGGGCAAGGC GGCCGACGAA 
GCTATGGAGG AGAGACGCAG GCTGCTTCAG AACAAGCGGA AGGAGCCGCC GGAAGATGTG 
GCCCAGCGGG CTGCCCGGCT CAAGACATTT CCTTGCAAGA GGTTTAAGGA GGGCACCTGT 
CAACGCGGGG ACCAGTGCTG CTACTCCCAC AGCCCCCCGA CACCCAGGGT TTCTGCTGAT 
GCTGCCCCTG ACTGTCCAGA GACCTCCAAC CAGACCCCCC CTGGACCTGG GGCTGCCGCT 
GGGCCAGGCA TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_024451591.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307359.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu51694
Clone ID Related Accession (Same CDS sequence)	XM_024451591.1 , XM_024451592.1 , NM_001351762.1 , XM_017026947.1 , NM_001351762.2 , XM_024451593.1
Accession Version	XM_024451591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307359.1
CDS	887..2083
Translation	MAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_024451591.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451591.1

ORF Insert Sequence:

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_024451592.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307360.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu51694
Clone ID Related Accession (Same CDS sequence)	XM_024451591.1 , XM_024451592.1 , NM_001351762.1 , XM_017026947.1 , NM_001351762.2 , XM_024451593.1
Accession Version	XM_024451592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307360.1
CDS	1663..2859
Translation	MAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_024451592.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451592.1

ORF Insert Sequence:

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_017026947.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882436.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu51694
Clone ID Related Accession (Same CDS sequence)	XM_024451591.1 , XM_024451592.1 , NM_001351762.1 , XM_017026947.1 , NM_001351762.2 , XM_024451593.1
Accession Version	XM_017026947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882436.1
CDS	1342..2538
Translation	MAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_017026947.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026947.1

ORF Insert Sequence:

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_024451593.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307361.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X7, mRNA.

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CloneID	OHu51694
Clone ID Related Accession (Same CDS sequence)	XM_024451591.1 , XM_024451592.1 , NM_001351762.1 , XM_017026947.1 , NM_001351762.2 , XM_024451593.1
Accession Version	XM_024451593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307361.1
CDS	688..1884
Translation	MAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_024451593.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451593.1

ORF Insert Sequence:

ATGGCGGTGT TGGCGGGGAA CAGCGGGGAG ACGTGCTACA GCAAGTACGG GGCCATGGCC 
CTCAAGAGCC GGGCCTGCCA CGAGATGGCC CTGAGAATCG TCCTGCACAG CCTGGACCTC 
CGCGCCAACT GCTACCAGCG CTTCGTGGTG CCGCTGCTCA GCATCAGCGC TGACTTCTAC 
GTGCGTGTTT TTGTCCGTGT CTTCACCGGC CAGGCCAAGG TCAAGGCCTC AGCCAGCAAG 
CAGGCGCTGG TGTTCCAGTG TGTGGGCTGC GGGGCCTTCC ACCTTCAGCG TCTCGGCAAA 
GCGTCAGGAG TCCCCAGCGG CCGGGCCAAG TTCTCTGCAG CCTGTGGTCC CCCTGTGACC 
CCCGAGTGTG AACACTGTGG GCAACGACAC CAGCTTGGTG GCCCCATGTG GGCAGAGCCC 
ATCCATGACC TGGATTTTGT GGGCCGTGTC CTGGAGGCTG TGAGCGCTAA CCCCGGCCGC 
TTCCACACCT CGGAGCGGAT CCGAGGGGTC CTGAGCGTCA TCACTGAGGA GCTCCCGGAC 
GTGCCTCTGT ACTACACCCT GGACCAGCTG AGCAGCACCA TCCACTGCAA CACACCAAGC 
CTCCTGCAGT TGCGGTCGGC CCTCCTCCAC GCTGACTTCC GGGTCTCACT CTCCCACGCC 
TGTAAGAACG CTGTGAAGAC GGATGCCCCT GCCTCTGCCC TCTGGGACAT CATGCGTTGC 
TGGGAGAAGG AATGTCCGGT GAAACGGGAG CGACTATCAG AGACTAGCCC AGCGTTCCGC 
ATTCTCAGTG TGGAGCCCAG GCTGCAGGCC AACTTCACCA TCCGGGAAGA TGCCAACCCC 
AGCTCCCGAC AGCGAGGACT CAAGCGCTTC CAGGCTAACC CGGAGGCCAA CTGGGGTCCC 
CGGCCTCGTG CCCGGCCAGG GGGCAAGGCG GCCGACGAAG CTATGGAGGA GAGACGCAGG 
CTGCTTCAGA ACAAGCGGAA GGAGCCGCCG GAAGATGTGG CCCAGCGGGC TGCCCGGCTC 
AAGACATTTC CTTGCAAGAG GTTTAAGGAG GGCACCTGTC AACGCGGGGA CCAGTGCTGC 
TACTCCCACA GCCCCCCGAC ACCCAGGGTT TCTGCTGATG CTGCCCCTGA CTGTCCAGAG 
ACCTCCAACC AGACCCCCCC TGGACCTGGG GCTGCCGCTG GGCCAGGCAT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_011528124.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526426.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.

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CloneID	OHu69540
Clone ID Related Accession (Same CDS sequence)	NM_001351761.1 , NM_001351761.2 , XM_011528124.1
Accession Version	XM_011528124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG GCACCGGGCC CTACGGAGAA GAACGTCCAC GTGAAGTCCA GGAGACGACA 
GTCACCGAGG GGGCTGCCAA AATCGCCTTT CCCAGTGCCA ACGAGGTCTT TTATAACCCG 
GTGCAGGAAT TCAATCGGGA CCTGACATGT GCTGTGATCA CCGAGTTTGC TCGCATTCAG 
CTTGGGGCCA AAGGAATCCA GATCAAGGTT CCAGGAGAGA AGGACACGCA AAAAGTGGTC 
GTGGACTTGT CAGAGCAAGA GGAGGAAAAG GTTGAACTGA AAGAGAGTGA AAACCTGGCC 
TCAGGAGACC AACCTCGCAC AGCGGCCGTG GGGGAGATCT GTGAGGAAGG CCTGCATGTG 
CTGGAAGGCC TGGCAGCTTC AGGCCTACGT TCCATTCGAT TTGCCCTAGA GGTGCCTGGG 
CTCAGATCTG TGGTTGCAAA CGATGCCTCC ACCCGGGCTG TGGATCTCAT ACGCCGGAAT 
GTCCAGCTCA ATGACGTGGC CCACCTGGTA CAGCCGAGCC AAGCAGATGC CCGGATGCTG 
ATGTACCAGC ACCAGAGGGT GTCGGAGAGG TTTGACGTCA TCGATCTGGA CCCCTATGGC 
AGCCCAGCCA CCTTCCTGGA TGCAGCTGTG CAGGCTGTGA GTGAAGGAGG GTTGCTGTGT 
GTGACCTGCA CAGACATGGC GGTGTTGGCG GGGAACAGCG GGGAGACGTG CTACAGCAAG 
TACGGGGCCA TGGCCCTCAA GAGCCGGGCC TGCCACGAGA TGGCCCTGAG AATCGTCCTG 
CACAGCCTGG ACCTCCGCGC CAACTGCTAC CAGCGCTTCG TGGTGCCGCT GCTCAGCATC 
AGCGCTGACT TCTACGTGCG TGTTTTTGTC CGTGTCTTCA CCGGCCAGGC CAAGGTCAAG 
GCCTCAGCCA GCAAGCAGGC GCTGGTGTTC CAGTGTGTGG GCTGCGGGGC CTTCCACCTT 
CAGCGTCTCG GCAAAGCGTC AGGAGTCCCC AGCGGCCGGG CCAAGTTCTC TGCAGCCTGT 
GGTCCCCCTG TGACCCCCGA GTGTGAACAC TGTGGGCAAC GACACCAGCT TGGTGGCCCC 
ATGTGGGCAG AGCCCATCCA TGACCTGGAT TTTGTGGGCC GTGTCCTGGA GGCTGTGAGC 
GCTAACCCCG GCCGCTTCCA CACCTCGGAG CGGATCCGAG GGGTCCTGAG CGTCATCACT 
GAGGAGCTCC CGGACGTGCC TCTGTACTAC ACCCTGGACC AGCTGAGCAG CACCATCCAC 
TGCAACACAC CAAGCCTCCT GCAGTTGCGG TCGGCCCTCC TCCACGCTGA CTTCCGGGTC 
TCACTCTCCC ACGCCTGTAA GAACGCTGTG AAGACGGATG CCCCTGCCTC TGCCCTCTGG 
GACATCATGC GTTGCTGGGA GAAGGAATGT CCGGTGAAAC GGGAGCGACT ATCAGAGACT 
AGCCCAGCGT TCCGCATTCT CAGTGTGGAG CCCAGGCTGC AGGCCAACTT CACCATCCGG 
GAAGATGCCA ACCCCAGCTC CCGACAGCGA GGACTCAAGC GCTTCCAGGC TAACCCGGAG 
GCCAACTGGG GTCCCCGGCC TCGTGCCCGG CCAGGGGGCA AGGCGGCCGA CGAAGCTATG 
GAGGAGAGAC GCAGGCTGCT TCAGAACAAG CGGAAGGAGC CGCCGGAAGA TGTGGCCCAG 
CGGGCTGCCC GGCTCAAGAC ATTTCCTTGC AAGAGGTTTA AGGAGGGCAC CTGTCAACGC 
GGGGACCAGT GCTGCTACTC CCACAGCCCC CCGACACCCA GGGTTTCTGC TGATGCTGCC 
CCTGACTGTC CAGAGACCTC CAACCAGACC CCCCCTGGAC CTGGGGCTGC CGCTGGGCCA 
GGCATAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526426.1
CDS	157..2028
Translation	MENGTGPYGEERPREVQETTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQPRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_011528124.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528124.1

ORF Insert Sequence:

ATGGAGAACG GCACCGGGCC CTACGGAGAA GAACGTCCAC GTGAAGTCCA GGAGACGACA 
GTCACCGAGG GGGCTGCCAA AATCGCCTTT CCCAGTGCCA ACGAGGTCTT TTATAACCCG 
GTGCAGGAAT TCAATCGGGA CCTGACATGT GCTGTGATCA CCGAGTTTGC TCGCATTCAG 
CTTGGGGCCA AAGGAATCCA GATCAAGGTT CCAGGAGAGA AGGACACGCA AAAAGTGGTC 
GTGGACTTGT CAGAGCAAGA GGAGGAAAAG GTTGAACTGA AAGAGAGTGA AAACCTGGCC 
TCAGGAGACC AACCTCGCAC AGCGGCCGTG GGGGAGATCT GTGAGGAAGG CCTGCATGTG 
CTGGAAGGCC TGGCAGCTTC AGGCCTACGT TCCATTCGAT TTGCCCTAGA GGTGCCTGGG 
CTCAGATCTG TGGTTGCAAA CGATGCCTCC ACCCGGGCTG TGGATCTCAT ACGCCGGAAT 
GTCCAGCTCA ATGACGTGGC CCACCTGGTA CAGCCGAGCC AAGCAGATGC CCGGATGCTG 
ATGTACCAGC ACCAGAGGGT GTCGGAGAGG TTTGACGTCA TCGATCTGGA CCCCTATGGC 
AGCCCAGCCA CCTTCCTGGA TGCAGCTGTG CAGGCTGTGA GTGAAGGAGG GTTGCTGTGT 
GTGACCTGCA CAGACATGGC GGTGTTGGCG GGGAACAGCG GGGAGACGTG CTACAGCAAG 
TACGGGGCCA TGGCCCTCAA GAGCCGGGCC TGCCACGAGA TGGCCCTGAG AATCGTCCTG 
CACAGCCTGG ACCTCCGCGC CAACTGCTAC CAGCGCTTCG TGGTGCCGCT GCTCAGCATC 
AGCGCTGACT TCTACGTGCG TGTTTTTGTC CGTGTCTTCA CCGGCCAGGC CAAGGTCAAG 
GCCTCAGCCA GCAAGCAGGC GCTGGTGTTC CAGTGTGTGG GCTGCGGGGC CTTCCACCTT 
CAGCGTCTCG GCAAAGCGTC AGGAGTCCCC AGCGGCCGGG CCAAGTTCTC TGCAGCCTGT 
GGTCCCCCTG TGACCCCCGA GTGTGAACAC TGTGGGCAAC GACACCAGCT TGGTGGCCCC 
ATGTGGGCAG AGCCCATCCA TGACCTGGAT TTTGTGGGCC GTGTCCTGGA GGCTGTGAGC 
GCTAACCCCG GCCGCTTCCA CACCTCGGAG CGGATCCGAG GGGTCCTGAG CGTCATCACT 
GAGGAGCTCC CGGACGTGCC TCTGTACTAC ACCCTGGACC AGCTGAGCAG CACCATCCAC 
TGCAACACAC CAAGCCTCCT GCAGTTGCGG TCGGCCCTCC TCCACGCTGA CTTCCGGGTC 
TCACTCTCCC ACGCCTGTAA GAACGCTGTG AAGACGGATG CCCCTGCCTC TGCCCTCTGG 
GACATCATGC GTTGCTGGGA GAAGGAATGT CCGGTGAAAC GGGAGCGACT ATCAGAGACT 
AGCCCAGCGT TCCGCATTCT CAGTGTGGAG CCCAGGCTGC AGGCCAACTT CACCATCCGG 
GAAGATGCCA ACCCCAGCTC CCGACAGCGA GGACTCAAGC GCTTCCAGGC TAACCCGGAG 
GCCAACTGGG GTCCCCGGCC TCGTGCCCGG CCAGGGGGCA AGGCGGCCGA CGAAGCTATG 
GAGGAGAGAC GCAGGCTGCT TCAGAACAAG CGGAAGGAGC CGCCGGAAGA TGTGGCCCAG 
CGGGCTGCCC GGCTCAAGAC ATTTCCTTGC AAGAGGTTTA AGGAGGGCAC CTGTCAACGC 
GGGGACCAGT GCTGCTACTC CCACAGCCCC CCGACACCCA GGGTTTCTGC TGATGCTGCC 
CCTGACTGTC CAGAGACCTC CAACCAGACC CCCCCTGGAC CTGGGGCTGC CGCTGGGCCA 
GGCATAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_024451590.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307358.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu88548
Clone ID Related Accession (Same CDS sequence)	XM_024451590.1 , XM_017026946.1
Accession Version	XM_024451590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGGGC CAAGTTCTCT GCAGCCTGTG GTCCCCCTGT GACCCCCGAG 
TGTGAACACT GTGGGCAACG ACACCAGCTT GGTGGCCCCA TGTGGGCAGA GCCCATCCAT 
GACCTGGATT TTGTGGGCCG TGTCCTGGAG GCTGTGAGCG CTAACCCCGG CCGCTTCCAC 
ACCTCGGAGC GGATCCGAGG GGTCCTGAGC GTCATCACTG AGGAGCTCCC GGACGTGCCT 
CTGTACTACA CCCTGGACCA GCTGAGCAGC ACCATCCACT GCAACACACC AAGCCTCCTG 
CAGTTGCGGT CGGCCCTCCT CCACGCTGAC TTCCGGGTCT CACTCTCCCA CGCCTGTAAG 
AACGCTGTGA AGACGGATGC CCCTGCCTCT GCCCTCTGGG ACATCATGCG TTGCTGGGAG 
AAGGAATGTC CGGTGAAACG GGAGCGACTA TCAGAGACTA GCCCAGCGTT CCGCATTCTC 
AGTGTGGAGC CCAGGCTGCA GGCCAACTTC ACCATCCGGG AAGATGCCAA CCCCAGCTCC 
CGACAGCGAG GACTCAAGCG CTTCCAGGCT AACCCGGAGG CCAACTGGGG TCCCCGGCCT 
CGTGCCCGGC CAGGGGGCAA GGCGGCCGAC GAAGCTATGG AGGAGAGACG CAGGCTGCTT 
CAGAACAAGC GGAAGGAGCC GCCGGAAGAT GTGGCCCAGC GGGCTGCCCG GCTCAAGACA 
TTTCCTTGCA AGAGGTTTAA GGAGGGCACC TGTCAACGCG GGGACCAGTG CTGCTACTCC 
CACAGCCCCC CGACACCCAG GGTTTCTGCT GATGCTGCCC CTGACTGTCC AGAGACCTCC 
AACCAGACCC CCCCTGGACC TGGGGCTGCC GCTGGGCCAG GCATAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307358.1
CDS	560..1810
Translation	MLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASARAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_024451590.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451590.1

ORF Insert Sequence:

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGGGC CAAGTTCTCT GCAGCCTGTG GTCCCCCTGT GACCCCCGAG 
TGTGAACACT GTGGGCAACG ACACCAGCTT GGTGGCCCCA TGTGGGCAGA GCCCATCCAT 
GACCTGGATT TTGTGGGCCG TGTCCTGGAG GCTGTGAGCG CTAACCCCGG CCGCTTCCAC 
ACCTCGGAGC GGATCCGAGG GGTCCTGAGC GTCATCACTG AGGAGCTCCC GGACGTGCCT 
CTGTACTACA CCCTGGACCA GCTGAGCAGC ACCATCCACT GCAACACACC AAGCCTCCTG 
CAGTTGCGGT CGGCCCTCCT CCACGCTGAC TTCCGGGTCT CACTCTCCCA CGCCTGTAAG 
AACGCTGTGA AGACGGATGC CCCTGCCTCT GCCCTCTGGG ACATCATGCG TTGCTGGGAG 
AAGGAATGTC CGGTGAAACG GGAGCGACTA TCAGAGACTA GCCCAGCGTT CCGCATTCTC 
AGTGTGGAGC CCAGGCTGCA GGCCAACTTC ACCATCCGGG AAGATGCCAA CCCCAGCTCC 
CGACAGCGAG GACTCAAGCG CTTCCAGGCT AACCCGGAGG CCAACTGGGG TCCCCGGCCT 
CGTGCCCGGC CAGGGGGCAA GGCGGCCGAC GAAGCTATGG AGGAGAGACG CAGGCTGCTT 
CAGAACAAGC GGAAGGAGCC GCCGGAAGAT GTGGCCCAGC GGGCTGCCCG GCTCAAGACA 
TTTCCTTGCA AGAGGTTTAA GGAGGGCACC TGTCAACGCG GGGACCAGTG CTGCTACTCC 
CACAGCCCCC CGACACCCAG GGTTTCTGCT GATGCTGCCC CTGACTGTCC AGAGACCTCC 
AACCAGACCC CCCCTGGACC TGGGGCTGCC GCTGGGCCAG GCATAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRMT1 ( XM_017026946.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882435.1 Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu88548
Clone ID Related Accession (Same CDS sequence)	XM_024451590.1 , XM_017026946.1
Accession Version	XM_017026946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGGGC CAAGTTCTCT GCAGCCTGTG GTCCCCCTGT GACCCCCGAG 
TGTGAACACT GTGGGCAACG ACACCAGCTT GGTGGCCCCA TGTGGGCAGA GCCCATCCAT 
GACCTGGATT TTGTGGGCCG TGTCCTGGAG GCTGTGAGCG CTAACCCCGG CCGCTTCCAC 
ACCTCGGAGC GGATCCGAGG GGTCCTGAGC GTCATCACTG AGGAGCTCCC GGACGTGCCT 
CTGTACTACA CCCTGGACCA GCTGAGCAGC ACCATCCACT GCAACACACC AAGCCTCCTG 
CAGTTGCGGT CGGCCCTCCT CCACGCTGAC TTCCGGGTCT CACTCTCCCA CGCCTGTAAG 
AACGCTGTGA AGACGGATGC CCCTGCCTCT GCCCTCTGGG ACATCATGCG TTGCTGGGAG 
AAGGAATGTC CGGTGAAACG GGAGCGACTA TCAGAGACTA GCCCAGCGTT CCGCATTCTC 
AGTGTGGAGC CCAGGCTGCA GGCCAACTTC ACCATCCGGG AAGATGCCAA CCCCAGCTCC 
CGACAGCGAG GACTCAAGCG CTTCCAGGCT AACCCGGAGG CCAACTGGGG TCCCCGGCCT 
CGTGCCCGGC CAGGGGGCAA GGCGGCCGAC GAAGCTATGG AGGAGAGACG CAGGCTGCTT 
CAGAACAAGC GGAAGGAGCC GCCGGAAGAT GTGGCCCAGC GGGCTGCCCG GCTCAAGACA 
TTTCCTTGCA AGAGGTTTAA GGAGGGCACC TGTCAACGCG GGGACCAGTG CTGCTACTCC 
CACAGCCCCC CGACACCCAG GGTTTCTGCT GATGCTGCCC CTGACTGTCC AGAGACCTCC 
AACCAGACCC CCCCTGGACC TGGGGCTGCC GCTGGGCCAG GCATAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882435.1
CDS	1015..2265
Translation	MLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASARAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPRVSADAAPDCPETSNQTPPGPGAAAGPGID

Target ORF information:

RefSeq Version	XM_017026946.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA methyltransferase 1 (TRMT1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026946.1

ORF Insert Sequence:

ATGCTGATGT ACCAGCACCA GAGGGTGTCG GAGAGGTTTG ACGTCATCGA TCTGGACCCC 
TATGGCAGCC CAGCCACCTT CCTGGATGCA GCTGTGCAGG CTGTGAGTGA AGGAGGGTTG 
CTGTGTGTGA CCTGCACAGA CATGGCGGTG TTGGCGGGGA ACAGCGGGGA GACGTGCTAC 
AGCAAGTACG GGGCCATGGC CCTCAAGAGC CGGGCCTGCC ACGAGATGGC CCTGAGAATC 
GTCCTGCACA GCCTGGACCT CCGCGCCAAC TGCTACCAGC GCTTCGTGGT GCCGCTGCTC 
AGCATCAGCG CTGACTTCTA CGTGCGTGTT TTTGTCCGTG TCTTCACCGG CCAGGCCAAG 
GTCAAGGCCT CAGCCAGGGC CAAGTTCTCT GCAGCCTGTG GTCCCCCTGT GACCCCCGAG 
TGTGAACACT GTGGGCAACG ACACCAGCTT GGTGGCCCCA TGTGGGCAGA GCCCATCCAT 
GACCTGGATT TTGTGGGCCG TGTCCTGGAG GCTGTGAGCG CTAACCCCGG CCGCTTCCAC 
ACCTCGGAGC GGATCCGAGG GGTCCTGAGC GTCATCACTG AGGAGCTCCC GGACGTGCCT 
CTGTACTACA CCCTGGACCA GCTGAGCAGC ACCATCCACT GCAACACACC AAGCCTCCTG 
CAGTTGCGGT CGGCCCTCCT CCACGCTGAC TTCCGGGTCT CACTCTCCCA CGCCTGTAAG 
AACGCTGTGA AGACGGATGC CCCTGCCTCT GCCCTCTGGG ACATCATGCG TTGCTGGGAG 
AAGGAATGTC CGGTGAAACG GGAGCGACTA TCAGAGACTA GCCCAGCGTT CCGCATTCTC 
AGTGTGGAGC CCAGGCTGCA GGCCAACTTC ACCATCCGGG AAGATGCCAA CCCCAGCTCC 
CGACAGCGAG GACTCAAGCG CTTCCAGGCT AACCCGGAGG CCAACTGGGG TCCCCGGCCT 
CGTGCCCGGC CAGGGGGCAA GGCGGCCGAC GAAGCTATGG AGGAGAGACG CAGGCTGCTT 
CAGAACAAGC GGAAGGAGCC GCCGGAAGAT GTGGCCCAGC GGGCTGCCCG GCTCAAGACA 
TTTCCTTGCA AGAGGTTTAA GGAGGGCACC TGTCAACGCG GGGACCAGTG CTGCTACTCC 
CACAGCCCCC CGACACCCAG GGTTTCTGCT GATGCTGCCC CTGACTGTCC AGAGACCTCC 
AACCAGACCC CCCCTGGACC TGGGGCTGCC GCTGGGCCAG GCATAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRMT1-Catalyzed tRNA Modifications Are Required for Redox Homeostasis To Ensure Proper Cellular Proliferation and Oxidative Stress Survival.
Molecular and cellular biology37(21)(2017 Nov)
Dewe JM,Fuller BL,Lentini JM,Kellner SM,Fu D

The Role of a Novel TRMT1 Gene Mutation and Rare GRM1 Gene Defect in Intellectual Disability in Two Azeri Families.
PloS one10(8)e0129631(2015)
Davarniya B,Hu H,Kahrizi K,Musante L,Fattahi Z,Hosseini M,Maqsoud F,Farajollahi R,Wienker TF,Ropers HH,Najmabadi H

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

The human tRNA(m(2)(2)G(26))dimethyltransferase: functional expression and characterization of a cloned hTRM1 gene.
Nucleic acids research28(18)3445-51(2000 Sep)
Liu J,Str?by KB

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TRMT1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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