BOLA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BOLA2
Entrez Gene ID	552900
Full Name	bolA family member 2
Synonyms	BOLA2A,BOLA2B,My016
General protein information	Preferred Names bolA family member 2 Names bolA-like protein 2 BolA-like protein 2 member A bolA homolog 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p11.2
Summary	This gene is located within a region of a segmental duplication on chromosome 16 and is identical to BOLA2B (bolA family member 2B). The product of this gene belongs to a family of proteins that are widely conserved and may be involved in iron maturation. Related pseudogenes are found multiple different chromosomes. Alternative splicing results in multiple transcript variants. Transcripts initiating at this locus may extend into downstream SMG1 pseudogene 6 (SMG1P6) and encode fusion proteins with a C-terminus related to SMG1 phosphatidylinositol 3-kinase-related kinase. A readthrough locus is represented with GeneID:107282092. [provided by RefSeq, Feb 2016].
Disorder MIM:	613182

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320579.1	NP_001307508.1	bolA-like protein 2 isoform 2
NM_001031827.1	NP_001026997.1	BolA-like protein 2
NM_001031827.2	NP_001026997.1	bolA-like protein 2 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC100363408	XP_006250179.1
Homo sapiens (human)	BOLA2B	NP_001034271.1
	BOLA2	XP_001110082.1
Homo sapiens (human)	BOLA2	NP_001026997.1

Gene Ontology
Bibliography

Related articles in PubMed

A Glutaredoxin?BolA Complex Serves as an Iron-Sulfur Cluster Chaperone for the Cytosolic Cluster Assembly Machinery.
Frey AG, Palenchar DJ, Wildemann JD, Philpott CC
The Journal of biological chemistry291(43)22344-22356(2016 10)

Emergence of a Homo sapiens-specific gene family and chromosome 16p11.2 CNV susceptibility.
Nuttle X, Giannuzzi G, Duyzend MH, Schraiber JG, Narvaiza I, Sudmant PH, Penn O, Chiatante G, Malig M, Huddleston J, Benner C, Camponeschi F, Ciofi-Baffoni S, Stessman HA, Marchetto MC, Denman L, Harshman L, Baker C, Raja A, Penewit K, Janke N, Tang WJ, Ventura M, Banci L, Antonacci F, Akey JM, Amemiya CT, Gage FH, Reymond A, Eichler EE
Nature536(7615)205-9(2016 08)

Elucidating the Molecular Function of Human BOLA2 in GRX3-Dependent Anamorsin Maturation Pathway.
Banci L, Camponeschi F, Ciofi-Baffoni S, Muzzioli R
Journal of the American Chemical Society137(51)16133-43(2015 Dec)

BOLA1 is an aerobic protein that prevents mitochondrial morphology changes induced by glutathione depletion.
Willems P, Wanschers BF, Esseling J, Szklarczyk R, Kudla U, Duarte I, Forkink M, Nooteboom M, Swarts H, Gloerich J, Nijtmans L, Koopman W, Huynen MA
Antioxidants & redox signaling18(2)129-38(2013 Jan)

Human glutaredoxin 3 forms [2Fe-2S]-bridged complexes with human BolA2.
Li H, Mapolelo DT, Randeniya S, Johnson MK, Outten CE
Biochemistry51(8)1687-96(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Glrx3 and BolA2 bound and facilitated Fe-S incorporation into Ciapin1, a [2Fe-2S] protein functioning early in the cytosolic Fe-S assembly pathway. Thus, Glrx3.BolA is a [2Fe-2S] chaperone complex capable of transferring [2Fe-2S] clusters to apoproteins in human cells.
Title: A Glutaredoxin?BolA Complex Serves as an Iron-Sulfur Cluster Chaperone for the Cytosolic Cluster Assembly Machinery.

BOLA2 shows Homo sapiens-specific duplication; this occurred around 280,000 years ago, with the duplication nearly fixed early in the human lineage
Title: Emergence of a Homo sapiens-specific gene family and chromosome 16p11.2 CNV susceptibility.

apo GRX3 and apo BOLA2 form a heterotrimeric complex, composed by two BOLA2 molecules and one GRX3 molecule
Title: Elucidating the Molecular Function of Human BOLA2 in GRX3-Dependent Anamorsin Maturation Pathway.

This study reported that all three human BolA proteins (hBolA1, hBolA2, and hBolA3) are novel non-classical secreted proteins identified with bioinformatics and molecular biology experiments.
Title: hBolA, novel non-classical secreted proteins, belonging to different BolA family with functional divergence.

The following BOLA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BOLA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29006	NM_001031827.2 Latest version!	Homo sapiens bolA family member 2 (BOLA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu101964	NM_001320579.1 Latest version!	Homo sapiens bolA family member 2 (BOLA2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu29006	NM_001031827.1	Homo sapiens bolA family member 2 (BOLA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BOLA2 ( NM_001031827.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001026997.1 Homo sapiens bolA family member 2 (BOLA2), mRNA.

Price & Availability↑

CloneID	OHu29006
Clone ID Related Accession (Same CDS sequence)	NM_001031827.1 , NM_001031827.2
Accession Version	NM_001031827.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	BolA-like protein 2
Comment

ATGGCAAGCG CGAAAAGCCT GGACCGCTGG AAAGCCCGGC TGCTTGAGGG CGGAAGTACT 
GCGTTGACGT ACGCCTTAGT AAGGGCGGAA GTGAGTTTTC CAGCGGAAGT GGCTCCTGTA 
AGGCAGCAAG GTAGCGTGGC CGGCGCCCGA GCTGGGGTTG TGTCCCTGCT GGGCTGCCGT 
TCCAGCTGGA CTGCCGCCAT GGAACTCAGC GCCGAATACC TCCGCGAGAA GCTGCAGCGG 
GACCTGGAGG CGGAGCATGT GGAGGTGGAG GACACGACCC TCAACCGTTG CTCCTGTAGC 
TTCCGAGTCC TGGTGGTGTC GGCCAAGTTC GAGGGGAAAC CGCTGCTTCA GAGACACAGG 
CTGGTGAACG CGTGCCTAGC AGAAGAGCTC CCGCACATCC ATGCCTTTGA ACAGAAAACC 
CTGACCCCAG ACCAGTGGGC ACGTGAGCGA CAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026997.1
Translation	MASAKSLDRWKARLLEGGSTALTYALVRAEVSFPAEVAPVRQQGSVAGARAGVVSLLGCRSSWTAAMELSAEYLREKLQRDLEAEHVEVEDTTLNRCSCSFRVLVVSAKFEGKPLLQRHRLVNACLAEELPHIHAFEQKTLTPDQWARERQK

Target ORF information:

RefSeq Version	NM_001031827.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens bolA family member 2 (BOLA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031827.1

ORF Insert Sequence:

ATGGCAAGCG CGAAAAGCCT GGACCGCTGG AAAGCCCGGC TGCTTGAGGG CGGAAGTACT 
GCGTTGACGT ACGCCTTAGT AAGGGCGGAA GTGAGTTTTC CAGCGGAAGT GGCTCCTGTA 
AGGCAGCAAG GTAGCGTGGC CGGCGCCCGA GCTGGGGTTG TGTCCCTGCT GGGCTGCCGT 
TCCAGCTGGA CTGCCGCCAT GGAACTCAGC GCCGAATACC TCCGCGAGAA GCTGCAGCGG 
GACCTGGAGG CGGAGCATGT GGAGGTGGAG GACACGACCC TCAACCGTTG CTCCTGTAGC 
TTCCGAGTCC TGGTGGTGTC GGCCAAGTTC GAGGGGAAAC CGCTGCTTCA GAGACACAGG 
CTGGTGAACG CGTGCCTAGC AGAAGAGCTC CCGCACATCC ATGCCTTTGA ACAGAAAACC 
CTGACCCCAG ACCAGTGGGC ACGTGAGCGA CAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BOLA2 ( NM_001031827.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001026997.1 Homo sapiens bolA family member 2 (BOLA2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29006
Clone ID Related Accession (Same CDS sequence)	NM_001031827.1 , NM_001031827.2
Accession Version	NM_001031827.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	bolA-like protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022832.1 and BU590412.1. On Mar 1, 2016 this sequence version replaced NM_001031827.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC022832.1, SRR1163655.446162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCG CGAAAAGCCT GGACCGCTGG AAAGCCCGGC TGCTTGAGGG CGGAAGTACT 
GCGTTGACGT ACGCCTTAGT AAGGGCGGAA GTGAGTTTTC CAGCGGAAGT GGCTCCTGTA 
AGGCAGCAAG GTAGCGTGGC CGGCGCCCGA GCTGGGGTTG TGTCCCTGCT GGGCTGCCGT 
TCCAGCTGGA CTGCCGCCAT GGAACTCAGC GCCGAATACC TCCGCGAGAA GCTGCAGCGG 
GACCTGGAGG CGGAGCATGT GGAGGTGGAG GACACGACCC TCAACCGTTG CTCCTGTAGC 
TTCCGAGTCC TGGTGGTGTC GGCCAAGTTC GAGGGGAAAC CGCTGCTTCA GAGACACAGG 
CTGGTGAACG CGTGCCTAGC AGAAGAGCTC CCGCACATCC ATGCCTTTGA ACAGAAAACC 
CTGACCCCAG ACCAGTGGGC ACGTGAGCGA CAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026997.1
CDS	514..972
Translation	MASAKSLDRWKARLLEGGSTALTYALVRAEVSFPAEVAPVRQQGSVAGARAGVVSLLGCRSSWTAAMELSAEYLREKLQRDLEAEHVEVEDTTLNRCSCSFRVLVVSAKFEGKPLLQRHRLVNACLAEELPHIHAFEQKTLTPDQWARERQK

Target ORF information:

RefSeq Version	NM_001031827.2
Organism	Homo sapiens(human)
Definition	Homo sapiens bolA family member 2 (BOLA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031827.2

ORF Insert Sequence:

ATGGCAAGCG CGAAAAGCCT GGACCGCTGG AAAGCCCGGC TGCTTGAGGG CGGAAGTACT 
GCGTTGACGT ACGCCTTAGT AAGGGCGGAA GTGAGTTTTC CAGCGGAAGT GGCTCCTGTA 
AGGCAGCAAG GTAGCGTGGC CGGCGCCCGA GCTGGGGTTG TGTCCCTGCT GGGCTGCCGT 
TCCAGCTGGA CTGCCGCCAT GGAACTCAGC GCCGAATACC TCCGCGAGAA GCTGCAGCGG 
GACCTGGAGG CGGAGCATGT GGAGGTGGAG GACACGACCC TCAACCGTTG CTCCTGTAGC 
TTCCGAGTCC TGGTGGTGTC GGCCAAGTTC GAGGGGAAAC CGCTGCTTCA GAGACACAGG 
CTGGTGAACG CGTGCCTAGC AGAAGAGCTC CCGCACATCC ATGCCTTTGA ACAGAAAACC 
CTGACCCCAG ACCAGTGGGC ACGTGAGCGA CAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BOLA2 ( NM_001320579.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307508.1 Homo sapiens bolA family member 2 (BOLA2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101964
Clone ID Related Accession (Same CDS sequence)	NM_001320579.1
Accession Version	NM_001320579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	bolA-like protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022832.1, BC104642.1 and BU590412.1. Transcript Variant: This variant (2) uses an alternate splice site, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct, shorter C-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BE220077.1, BC104642.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCG CGAAAAGCCT GGACCGCTGG AAAGCCCGGC TGCTTGAGGG CGGAAGTACT 
GCGTTGACGT ACGCCTTAGT AAGGGCGGAA GTGAGTTTTC CAGCGGAAGT GGCTCCTGTA 
AGGCAGCAAG GTAGCGTGGC CGGCGCCCGA GCTGGGGTTG TGTCCCTGCT GGGCTGCCGT 
TCCAGCTGGA CTGCCGCCAT GGAACTCAGC GCCGAATACC TCCGCGAGAA GCTGCAGCGG 
GACCTGGAGG CGGAGCATGT GCTTCCGAGT CCTGGTGGTG TCGGCCAAGT TCGAGGGGAA 
ACCGCTGCTT CAGAGACACA GGCTGGTGAA CGCGTGCCTA GCAGAAGAGC TCCCGCACAT 
CCATGCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307508.1
CDS	514..885
Translation	MASAKSLDRWKARLLEGGSTALTYALVRAEVSFPAEVAPVRQQGSVAGARAGVVSLLGCRSSWTAAMELSAEYLREKLQRDLEAEHVLPSPGGVGQVRGETAASETQAGERVPSRRAPAHPCL

Target ORF information:

RefSeq Version	NM_001320579.1
Organism	Homo sapiens(human)
Definition	Homo sapiens bolA family member 2 (BOLA2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320579.1

ORF Insert Sequence:

ATGGCAAGCG CGAAAAGCCT GGACCGCTGG AAAGCCCGGC TGCTTGAGGG CGGAAGTACT 
GCGTTGACGT ACGCCTTAGT AAGGGCGGAA GTGAGTTTTC CAGCGGAAGT GGCTCCTGTA 
AGGCAGCAAG GTAGCGTGGC CGGCGCCCGA GCTGGGGTTG TGTCCCTGCT GGGCTGCCGT 
TCCAGCTGGA CTGCCGCCAT GGAACTCAGC GCCGAATACC TCCGCGAGAA GCTGCAGCGG 
GACCTGGAGG CGGAGCATGT GCTTCCGAGT CCTGGTGGTG TCGGCCAAGT TCGAGGGGAA 
ACCGCTGCTT CAGAGACACA GGCTGGTGAA CGCGTGCCTA GCAGAAGAGC TCCCGCACAT 
CCATGCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Glutaredoxin?BolA Complex Serves as an Iron-Sulfur Cluster Chaperone for the Cytosolic Cluster Assembly Machinery.
The Journal of biological chemistry291(43)22344-22356(2016 10)
Frey AG,Palenchar DJ,Wildemann JD,Philpott CC

Emergence of a Homo sapiens-specific gene family and chromosome 16p11.2 CNV susceptibility.
Nature536(7615)205-9(2016 08)
Nuttle X,Giannuzzi G,Duyzend MH,Schraiber JG,Narvaiza I,Sudmant PH,Penn O,Chiatante G,Malig M,Huddleston J,Benner C,Camponeschi F,Ciofi-Baffoni S,Stessman HA,Marchetto MC,Denman L,Harshman L,Baker C,Raja A,Penewit K,Janke N,Tang WJ,Ventura M,Banci L,Antonacci F,Akey JM,Amemiya CT,Gage FH,Reymond A,Eichler EE

Elucidating the Molecular Function of Human BOLA2 in GRX3-Dependent Anamorsin Maturation Pathway.
Journal of the American Chemical Society137(51)16133-43(2015 Dec)
Banci L,Camponeschi F,Ciofi-Baffoni S,Muzzioli R

BOLA1 is an aerobic protein that prevents mitochondrial morphology changes induced by glutathione depletion.
Antioxidants & redox signaling18(2)129-38(2013 Jan)
Willems P,Wanschers BF,Esseling J,Szklarczyk R,Kudla U,Duarte I,Forkink M,Nooteboom M,Swarts H,Gloerich J,Nijtmans L,Koopman W,Huynen MA

Human glutaredoxin 3 forms [2Fe-2S]-bridged complexes with human BolA2.
Biochemistry51(8)1687-96(2012 Feb)
Li H,Mapolelo DT,Randeniya S,Johnson MK,Outten CE

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BOLA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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