PPP2R5A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP2R5A
Entrez Gene ID	5525
Full Name	protein phosphatase 2 regulatory subunit B'alpha
Synonyms	B56A,B56alpha,PR61A
General protein information	Preferred Names protein phosphatase 2 regulatory subunit B'alpha Names serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform PP2A B subunit isoform B'-alpha PP2A B subunit isoform B56-alpha PP2A B subunit isoform PR61-alpha PP2A B subunit isoform R5-alpha PP2A, B subunit, B' alpha isoform PP2A, B subunit, B56 alpha isoform PP2A, B subunit, PR61 alpha isoform PP2A, B subunit, R5 alpha isoform PR61alpha protein phosphatase 2 regulatory subunit B', alpha protein phosphatase 2, regulatory subunit B (B56), alpha isoform protein phosphatase 2, regulatory subunit B', alpha isoform serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alpha isoform
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.3
Summary	The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010].
Disorder MIM:	601643

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199756.2	NP_001186685.1	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 2
NM_006243.3	NP_006234.1	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 1
NM_001199756.1	NP_001186685.1	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 2
NM_006243.4	NP_006234.1	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ppp2r5a	NP_659129.2
Gallus gallus (chicken)	PPP2R5A	XP_419432.2
Oryza sativa (rice)	Os04g0485100	NP_001053130.1
Pan troglodytes (chimpanzee)	PPP2R5A	XP_001151388.3
Danio rerio (zebrafish)	ppp2r5a	XP_690932.3
Arabidopsis thaliana (thale cress)	ATB' GAMMA	NP_567464.1
Oryza sativa (rice)	Os03g0844500	NP_001051871.1
Oryza sativa (rice)	Os08g0122000	NP_001060884.1
	PPP2R5A	XP_001106979.2
Canis lupus familiaris (dog)	PPP2R5A	XP_005622406.1
Oryza sativa (rice)	Os07g0274800	NP_001059361.1
Xenopus tropicalis (tropical clawed frog)	ppp2r5a	NP_001072157.1
Homo sapiens (human)	PPP2R5A	NP_006234.1
Bos taurus (cattle)	PPP2R5A	NP_001075197.1
Rattus norvegicus (Norway rat)	Ppp2r5a	NP_001101361.1
Arabidopsis thaliana (thale cress)	AT3G21650	NP_188802.1

Gene Ontology
Bibliography

Related articles in PubMed

PPP2R5A: A multirole protein phosphatase subunit in regulating cancer development.
Mao Z, Liu C, Lin X, Sun B, Su C
Cancer letters414222-229(2018 Feb)

MicroRNA-218 promotes cisplatin resistance in oral cancer via the PPP2R5A/Wnt signaling pathway.
Zhuang Z, Hu F, Hu J, Wang C, Hou J, Yu Z, Wang TT, Liu X, Huang H
Oncology reports38(4)2051-2061(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The PP2A
Lee SJ, Rodriguez-Bravo V, Kim H, Datta S, Foley EA
Journal of cell science130(10)1760-1771(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cyclin E is specifically dephosphorylated at S384 by the PP2A-B56 phosphatase, thereby uncoupling cyclin E degradation from cyclin E-CDK2 activity
Title: The PP2A-B56 Phosphatase Opposes Cyclin E Autocatalytic Degradation via Site-Specific Dephosphorylation.

Results report that BubR1 and RepoMan bind directly to PP2A-B56 using an LSPIxE short linear motif (SLiM), where phosphorylation of the Ser residue enhances binding. RepoMan and BubR1 bind B56 using both hydrophobic and electrostatic interactions.
Title: Expanding the PP2A Interactome by Defining a B56-Specific SLiM.

structure of the PP2A B56-BubR1 complex provides important insights into how the B56 subunit directs the recruitment of PP2A to specific targets.
Title: Crystal structure of a PP2A B56-BubR1 complex and its implications for PP2A substrate recruitment and localization.

results suggest that PKCalpha can modify PP2A activity by phosphorylation of B56alpha at Ser(41). This interplay between PKCalpha and PP2A represents a new mechanism to regulate important cellular functions like cellular Ca(2+) homeostasis.
Title: Protein phosphatase 2A is regulated by protein kinase C? (PKC?)-dependent phosphorylation of its targeting subunit B56? at Ser41.

PP2A regulatory subunit Balpha controls endothelial contractility and vessel lumen integrity via regulation of HDAC7.
Title: PP2A regulatory subunit B? controls endothelial contractility and vessel lumen integrity via regulation of HDAC7.

The following PPP2R5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP2R5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14530	NM_006243.4 Latest version!	Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu13791	NM_001199756.2 Latest version!	Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu13791	NM_001199756.1	Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu14530	NM_006243.3	Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP2R5A ( NM_006243.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006234.1 Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14530
Clone ID Related Accession (Same CDS sequence)	NM_006243.3 , NM_006243.4
Accession Version	NM_006243.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-30
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451060.13, L42373.1 and AA425592.1. On Dec 16, 2010 this sequence version replaced NM_006243.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L42373.1, SRR1163655.264353.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCGT CGTCGCCGCC GGCGGGGGCT GCCAGCGCCG CCATCTCGGC CTCGGAGAAA 
GTGGACGGCT TCACCCGGAA ATCGGTCCGC AAGGCGCAGA GGCAGAAGCG CTCCCAGGGC 
TCGTCGCAGT TTCGCAGCCA GGGCAGCCAG GCAGAGCTGC ACCCGCTGCC CCAGCTCAAA 
GATGCCACTT CAAATGAACA ACAAGAGCTT TTCTGTCAGA AGTTGCAGCA GTGTTGTATA 
CTGTTTGATT TCATGGACTC TGTTTCAGAC TTGAAGAGCA AAGAAATTAA AAGAGCAACA 
CTGAATGAAC TGGTTGAGTA TGTTTCAACT AATCGTGGTG TAATTGTTGA ATCAGCGTAT 
TCTGATATAG TAAAAATGAT CAGTGCTAAC ATCTTCCGTA CACTTCCTCC AAGTGATAAT 
CCAGATTTTG ATCCAGAAGA GGATGAACCC ACGCTTGAGG CCTCTTGGCC TCACATACAG 
TTGGTATATG AATTCTTCTT GAGATTTTTG GAGAGCCCTG ATTTCCAGCC TAGCATTGCA 
AAACGATACA TTGATCAGAA ATTCGTACAA CAGCTCCTGG AGCTTTTTGA TAGTGAAGAT 
CCCAGAGAAC GTGACTTCCT GAAGACTGTT CTGCACCGAA TTTATGGGAA ATTTCTTGGA 
TTAAGAGCAT TCATCAGAAA ACAAATTAAC AACATTTTCC TCAGGTTTAT ATATGAAACA 
GAACATTTCA ATGGTGTTGC TGAACTTCTT GAAATATTAG GAAGTATTAT CAATGGCTTT 
GCATTGCCAC TGAAAGCAGA ACATAAACAA TTTCTAATGA AGGTTCTTAT TCCTATGCAT 
ACTGCAAAAG GATTAGCTTT GTTTCATGCT CAGCTAGCAT ATTGTGTTGT ACAGTTCCTG 
GAGAAAGATA CAACACTAAC AGAGCCAGTG ATCAGAGGAC TGCTGAAATT TTGGCCAAAA 
ACCTGCAGTC AGAAAGAGGT GATGTTTTTA GGAGAAATTG AAGAAATCTT AGATGTCATT 
GAACCAACAC AGTTCAAAAA AATTGAAGAG CCACTTTTCA AGCAGATATC CAAGTGTGTA 
TCCAGTTCTC ATTTTCAGGT TGCAGAAAGG GCATTGTACT TCTGGAATAA CGAATATATT 
CTTAGTTTGA TTGAGGAGAA CATTGATAAA ATTCTGCCAA TTATGTTTGC CAGTTTGTAC 
AAAATTTCCA AAGAACACTG GAATCCGACC ATTGTAGCAC TGGTATACAA TGTGCTGAAA 
ACCCTAATGG AAATGAATGG CAAGCTTTTC GATGACCTTA CTAGCTCATA CAAAGCTGAA 
AGACAGAGAG AGAAAAAGAA GGAATTGGAA CGTGAAGAAT TATGGAAAAA ATTAGAGGAG 
CTAAAGCTAA AGAAAGCTCT AGAAAAACAG AATAGTGCTT ACAACATGCA CAGTATTCTC 
AGCAATACAA GTGCCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006234.1
CDS	575..2035
Translation	MSSSSPPAGAASAAISASEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHPLPQLKDATSNEQQELFCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE

Target ORF information:

RefSeq Version	NM_006243.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006243.3

ORF Insert Sequence:

ATGTCGTCGT CGTCGCCGCC GGCGGGGGCT GCCAGCGCCG CCATCTCGGC CTCGGAGAAA 
GTGGACGGCT TCACCCGGAA ATCGGTCCGC AAGGCGCAGA GGCAGAAGCG CTCCCAGGGC 
TCGTCGCAGT TTCGCAGCCA GGGCAGCCAG GCAGAGCTGC ACCCGCTGCC CCAGCTCAAA 
GATGCCACTT CAAATGAACA ACAAGAGCTT TTCTGTCAGA AGTTGCAGCA GTGTTGTATA 
CTGTTTGATT TCATGGACTC TGTTTCAGAC TTGAAGAGCA AAGAAATTAA AAGAGCAACA 
CTGAATGAAC TGGTTGAGTA TGTTTCAACT AATCGTGGTG TAATTGTTGA ATCAGCGTAT 
TCTGATATAG TAAAAATGAT CAGTGCTAAC ATCTTCCGTA CACTTCCTCC AAGTGATAAT 
CCAGATTTTG ATCCAGAAGA GGATGAACCC ACGCTTGAGG CCTCTTGGCC TCACATACAG 
TTGGTATATG AATTCTTCTT GAGATTTTTG GAGAGCCCTG ATTTCCAGCC TAGCATTGCA 
AAACGATACA TTGATCAGAA ATTCGTACAA CAGCTCCTGG AGCTTTTTGA TAGTGAAGAT 
CCCAGAGAAC GTGACTTCCT GAAGACTGTT CTGCACCGAA TTTATGGGAA ATTTCTTGGA 
TTAAGAGCAT TCATCAGAAA ACAAATTAAC AACATTTTCC TCAGGTTTAT ATATGAAACA 
GAACATTTCA ATGGTGTTGC TGAACTTCTT GAAATATTAG GAAGTATTAT CAATGGCTTT 
GCATTGCCAC TGAAAGCAGA ACATAAACAA TTTCTAATGA AGGTTCTTAT TCCTATGCAT 
ACTGCAAAAG GATTAGCTTT GTTTCATGCT CAGCTAGCAT ATTGTGTTGT ACAGTTCCTG 
GAGAAAGATA CAACACTAAC AGAGCCAGTG ATCAGAGGAC TGCTGAAATT TTGGCCAAAA 
ACCTGCAGTC AGAAAGAGGT GATGTTTTTA GGAGAAATTG AAGAAATCTT AGATGTCATT 
GAACCAACAC AGTTCAAAAA AATTGAAGAG CCACTTTTCA AGCAGATATC CAAGTGTGTA 
TCCAGTTCTC ATTTTCAGGT TGCAGAAAGG GCATTGTACT TCTGGAATAA CGAATATATT 
CTTAGTTTGA TTGAGGAGAA CATTGATAAA ATTCTGCCAA TTATGTTTGC CAGTTTGTAC 
AAAATTTCCA AAGAACACTG GAATCCGACC ATTGTAGCAC TGGTATACAA TGTGCTGAAA 
ACCCTAATGG AAATGAATGG CAAGCTTTTC GATGACCTTA CTAGCTCATA CAAAGCTGAA 
AGACAGAGAG AGAAAAAGAA GGAATTGGAA CGTGAAGAAT TATGGAAAAA ATTAGAGGAG 
CTAAAGCTAA AGAAAGCTCT AGAAAAACAG AATAGTGCTT ACAACATGCA CAGTATTCTC 
AGCAATACAA GTGCCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R5A ( NM_006243.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006234.1 Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14530
Clone ID Related Accession (Same CDS sequence)	NM_006243.3 , NM_006243.4
Accession Version	NM_006243.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL451060.13 and L42373.1. On Nov 22, 2018 this sequence version replaced NM_006243.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.264353.1, L42373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261461.7/ ENSP00000261461.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGTCGT CGTCGCCGCC GGCGGGGGCT GCCAGCGCCG CCATCTCGGC CTCGGAGAAA 
GTGGACGGCT TCACCCGGAA ATCGGTCCGC AAGGCGCAGA GGCAGAAGCG CTCCCAGGGC 
TCGTCGCAGT TTCGCAGCCA GGGCAGCCAG GCAGAGCTGC ACCCGCTGCC CCAGCTCAAA 
GATGCCACTT CAAATGAACA ACAAGAGCTT TTCTGTCAGA AGTTGCAGCA GTGTTGTATA 
CTGTTTGATT TCATGGACTC TGTTTCAGAC TTGAAGAGCA AAGAAATTAA AAGAGCAACA 
CTGAATGAAC TGGTTGAGTA TGTTTCAACT AATCGTGGTG TAATTGTTGA ATCAGCGTAT 
TCTGATATAG TAAAAATGAT CAGTGCTAAC ATCTTCCGTA CACTTCCTCC AAGTGATAAT 
CCAGATTTTG ATCCAGAAGA GGATGAACCC ACGCTTGAGG CCTCTTGGCC TCACATACAG 
TTGGTATATG AATTCTTCTT GAGATTTTTG GAGAGCCCTG ATTTCCAGCC TAGCATTGCA 
AAACGATACA TTGATCAGAA ATTCGTACAA CAGCTCCTGG AGCTTTTTGA TAGTGAAGAT 
CCCAGAGAAC GTGACTTCCT GAAGACTGTT CTGCACCGAA TTTATGGGAA ATTTCTTGGA 
TTAAGAGCAT TCATCAGAAA ACAAATTAAC AACATTTTCC TCAGGTTTAT ATATGAAACA 
GAACATTTCA ATGGTGTTGC TGAACTTCTT GAAATATTAG GAAGTATTAT CAATGGCTTT 
GCATTGCCAC TGAAAGCAGA ACATAAACAA TTTCTAATGA AGGTTCTTAT TCCTATGCAT 
ACTGCAAAAG GATTAGCTTT GTTTCATGCT CAGCTAGCAT ATTGTGTTGT ACAGTTCCTG 
GAGAAAGATA CAACACTAAC AGAGCCAGTG ATCAGAGGAC TGCTGAAATT TTGGCCAAAA 
ACCTGCAGTC AGAAAGAGGT GATGTTTTTA GGAGAAATTG AAGAAATCTT AGATGTCATT 
GAACCAACAC AGTTCAAAAA AATTGAAGAG CCACTTTTCA AGCAGATATC CAAGTGTGTA 
TCCAGTTCTC ATTTTCAGGT TGCAGAAAGG GCATTGTACT TCTGGAATAA CGAATATATT 
CTTAGTTTGA TTGAGGAGAA CATTGATAAA ATTCTGCCAA TTATGTTTGC CAGTTTGTAC 
AAAATTTCCA AAGAACACTG GAATCCGACC ATTGTAGCAC TGGTATACAA TGTGCTGAAA 
ACCCTAATGG AAATGAATGG CAAGCTTTTC GATGACCTTA CTAGCTCATA CAAAGCTGAA 
AGACAGAGAG AGAAAAAGAA GGAATTGGAA CGTGAAGAAT TATGGAAAAA ATTAGAGGAG 
CTAAAGCTAA AGAAAGCTCT AGAAAAACAG AATAGTGCTT ACAACATGCA CAGTATTCTC 
AGCAATACAA GTGCCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006234.1
CDS	702..2162
Translation	MSSSSPPAGAASAAISASEKVDGFTRKSVRKAQRQKRSQGSSQFRSQGSQAELHPLPQLKDATSNEQQELFCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE

Target ORF information:

RefSeq Version	NM_006243.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006243.4

ORF Insert Sequence:

ATGTCGTCGT CGTCGCCGCC GGCGGGGGCT GCCAGCGCCG CCATCTCGGC CTCGGAGAAA 
GTGGACGGCT TCACCCGGAA ATCGGTCCGC AAGGCGCAGA GGCAGAAGCG CTCCCAGGGC 
TCGTCGCAGT TTCGCAGCCA GGGCAGCCAG GCAGAGCTGC ACCCGCTGCC CCAGCTCAAA 
GATGCCACTT CAAATGAACA ACAAGAGCTT TTCTGTCAGA AGTTGCAGCA GTGTTGTATA 
CTGTTTGATT TCATGGACTC TGTTTCAGAC TTGAAGAGCA AAGAAATTAA AAGAGCAACA 
CTGAATGAAC TGGTTGAGTA TGTTTCAACT AATCGTGGTG TAATTGTTGA ATCAGCGTAT 
TCTGATATAG TAAAAATGAT CAGTGCTAAC ATCTTCCGTA CACTTCCTCC AAGTGATAAT 
CCAGATTTTG ATCCAGAAGA GGATGAACCC ACGCTTGAGG CCTCTTGGCC TCACATACAG 
TTGGTATATG AATTCTTCTT GAGATTTTTG GAGAGCCCTG ATTTCCAGCC TAGCATTGCA 
AAACGATACA TTGATCAGAA ATTCGTACAA CAGCTCCTGG AGCTTTTTGA TAGTGAAGAT 
CCCAGAGAAC GTGACTTCCT GAAGACTGTT CTGCACCGAA TTTATGGGAA ATTTCTTGGA 
TTAAGAGCAT TCATCAGAAA ACAAATTAAC AACATTTTCC TCAGGTTTAT ATATGAAACA 
GAACATTTCA ATGGTGTTGC TGAACTTCTT GAAATATTAG GAAGTATTAT CAATGGCTTT 
GCATTGCCAC TGAAAGCAGA ACATAAACAA TTTCTAATGA AGGTTCTTAT TCCTATGCAT 
ACTGCAAAAG GATTAGCTTT GTTTCATGCT CAGCTAGCAT ATTGTGTTGT ACAGTTCCTG 
GAGAAAGATA CAACACTAAC AGAGCCAGTG ATCAGAGGAC TGCTGAAATT TTGGCCAAAA 
ACCTGCAGTC AGAAAGAGGT GATGTTTTTA GGAGAAATTG AAGAAATCTT AGATGTCATT 
GAACCAACAC AGTTCAAAAA AATTGAAGAG CCACTTTTCA AGCAGATATC CAAGTGTGTA 
TCCAGTTCTC ATTTTCAGGT TGCAGAAAGG GCATTGTACT TCTGGAATAA CGAATATATT 
CTTAGTTTGA TTGAGGAGAA CATTGATAAA ATTCTGCCAA TTATGTTTGC CAGTTTGTAC 
AAAATTTCCA AAGAACACTG GAATCCGACC ATTGTAGCAC TGGTATACAA TGTGCTGAAA 
ACCCTAATGG AAATGAATGG CAAGCTTTTC GATGACCTTA CTAGCTCATA CAAAGCTGAA 
AGACAGAGAG AGAAAAAGAA GGAATTGGAA CGTGAAGAAT TATGGAAAAA ATTAGAGGAG 
CTAAAGCTAA AGAAAGCTCT AGAAAAACAG AATAGTGCTT ACAACATGCA CAGTATTCTC 
AGCAATACAA GTGCCGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R5A ( NM_001199756.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186685.1 Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13791
Clone ID Related Accession (Same CDS sequence)	NM_001199756.2 , NM_001199756.1
Accession Version	NM_001199756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302202.1, BP326450.1 and L42373.1. On May 31, 2019 this sequence version replaced NM_001199756.1. Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302202.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGATAATGA ATGCCACTTC AAATGAACAA CAAGAGCTTT TCTGTCAGAA GTTGCAGCAG 
TGTTGTATAC TGTTTGATTT CATGGACTCT GTTTCAGACT TGAAGAGCAA AGAAATTAAA 
AGAGCAACAC TGAATGAACT GGTTGAGTAT GTTTCAACTA ATCGTGGTGT AATTGTTGAA 
TCAGCGTATT CTGATATAGT AAAAATGATC AGTGCTAACA TCTTCCGTAC ACTTCCTCCA 
AGTGATAATC CAGATTTTGA TCCAGAAGAG GATGAACCCA CGCTTGAGGC CTCTTGGCCT 
CACATACAGT TGGTATATGA ATTCTTCTTG AGATTTTTGG AGAGCCCTGA TTTCCAGCCT 
AGCATTGCAA AACGATACAT TGATCAGAAA TTCGTACAAC AGCTCCTGGA GCTTTTTGAT 
AGTGAAGATC CCAGAGAACG TGACTTCCTG AAGACTGTTC TGCACCGAAT TTATGGGAAA 
TTTCTTGGAT TAAGAGCATT CATCAGAAAA CAAATTAACA ACATTTTCCT CAGGTTTATA 
TATGAAACAG AACATTTCAA TGGTGTTGCT GAACTTCTTG AAATATTAGG AAGTATTATC 
AATGGCTTTG CATTGCCACT GAAAGCAGAA CATAAACAAT TTCTAATGAA GGTTCTTATT 
CCTATGCATA CTGCAAAAGG ATTAGCTTTG TTTCATGCTC AGCTAGCATA TTGTGTTGTA 
CAGTTCCTGG AGAAAGATAC AACACTAACA GAGCCAGTGA TCAGAGGACT GCTGAAATTT 
TGGCCAAAAA CCTGCAGTCA GAAAGAGGTG ATGTTTTTAG GAGAAATTGA AGAAATCTTA 
GATGTCATTG AACCAACACA GTTCAAAAAA ATTGAAGAGC CACTTTTCAA GCAGATATCC 
AAGTGTGTAT CCAGTTCTCA TTTTCAGGTT GCAGAAAGGG CATTGTACTT CTGGAATAAC 
GAATATATTC TTAGTTTGAT TGAGGAGAAC ATTGATAAAA TTCTGCCAAT TATGTTTGCC 
AGTTTGTACA AAATTTCCAA AGAACACTGG AATCCGACCA TTGTAGCACT GGTATACAAT 
GTGCTGAAAA CCCTAATGGA AATGAATGGC AAGCTTTTCG ATGACCTTAC TAGCTCATAC 
AAAGCTGAAA GACAGAGAGA GAAAAAGAAG GAATTGGAAC GTGAAGAATT ATGGAAAAAA 
TTAGAGGAGC TAAAGCTAAA GAAAGCTCTA GAAAAACAGA ATAGTGCTTA CAACATGCAC 
AGTATTCTCA GCAATACAAG TGCCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186685.1
CDS	226..1515
Translation	MIMNATSNEQQELFCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE

Target ORF information:

RefSeq Version	NM_001199756.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199756.2

ORF Insert Sequence:

ATGATAATGA ATGCCACTTC AAATGAACAA CAAGAGCTTT TCTGTCAGAA GTTGCAGCAG 
TGTTGTATAC TGTTTGATTT CATGGACTCT GTTTCAGACT TGAAGAGCAA AGAAATTAAA 
AGAGCAACAC TGAATGAACT GGTTGAGTAT GTTTCAACTA ATCGTGGTGT AATTGTTGAA 
TCAGCGTATT CTGATATAGT AAAAATGATC AGTGCTAACA TCTTCCGTAC ACTTCCTCCA 
AGTGATAATC CAGATTTTGA TCCAGAAGAG GATGAACCCA CGCTTGAGGC CTCTTGGCCT 
CACATACAGT TGGTATATGA ATTCTTCTTG AGATTTTTGG AGAGCCCTGA TTTCCAGCCT 
AGCATTGCAA AACGATACAT TGATCAGAAA TTCGTACAAC AGCTCCTGGA GCTTTTTGAT 
AGTGAAGATC CCAGAGAACG TGACTTCCTG AAGACTGTTC TGCACCGAAT TTATGGGAAA 
TTTCTTGGAT TAAGAGCATT CATCAGAAAA CAAATTAACA ACATTTTCCT CAGGTTTATA 
TATGAAACAG AACATTTCAA TGGTGTTGCT GAACTTCTTG AAATATTAGG AAGTATTATC 
AATGGCTTTG CATTGCCACT GAAAGCAGAA CATAAACAAT TTCTAATGAA GGTTCTTATT 
CCTATGCATA CTGCAAAAGG ATTAGCTTTG TTTCATGCTC AGCTAGCATA TTGTGTTGTA 
CAGTTCCTGG AGAAAGATAC AACACTAACA GAGCCAGTGA TCAGAGGACT GCTGAAATTT 
TGGCCAAAAA CCTGCAGTCA GAAAGAGGTG ATGTTTTTAG GAGAAATTGA AGAAATCTTA 
GATGTCATTG AACCAACACA GTTCAAAAAA ATTGAAGAGC CACTTTTCAA GCAGATATCC 
AAGTGTGTAT CCAGTTCTCA TTTTCAGGTT GCAGAAAGGG CATTGTACTT CTGGAATAAC 
GAATATATTC TTAGTTTGAT TGAGGAGAAC ATTGATAAAA TTCTGCCAAT TATGTTTGCC 
AGTTTGTACA AAATTTCCAA AGAACACTGG AATCCGACCA TTGTAGCACT GGTATACAAT 
GTGCTGAAAA CCCTAATGGA AATGAATGGC AAGCTTTTCG ATGACCTTAC TAGCTCATAC 
AAAGCTGAAA GACAGAGAGA GAAAAAGAAG GAATTGGAAC GTGAAGAATT ATGGAAAAAA 
TTAGAGGAGC TAAAGCTAAA GAAAGCTCTA GAAAAACAGA ATAGTGCTTA CAACATGCAC 
AGTATTCTCA GCAATACAAG TGCCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP2R5A ( NM_001199756.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186685.1 Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 2, mRNA.

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CloneID	OHu13791
Clone ID Related Accession (Same CDS sequence)	NM_001199756.2 , NM_001199756.1
Accession Version	NM_001199756.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Homo sapiens(human)
Product	serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302202.1, BP326450.1, L42373.1 and AA425592.1. Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302202.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGATAATGA ATGCCACTTC AAATGAACAA CAAGAGCTTT TCTGTCAGAA GTTGCAGCAG 
TGTTGTATAC TGTTTGATTT CATGGACTCT GTTTCAGACT TGAAGAGCAA AGAAATTAAA 
AGAGCAACAC TGAATGAACT GGTTGAGTAT GTTTCAACTA ATCGTGGTGT AATTGTTGAA 
TCAGCGTATT CTGATATAGT AAAAATGATC AGTGCTAACA TCTTCCGTAC ACTTCCTCCA 
AGTGATAATC CAGATTTTGA TCCAGAAGAG GATGAACCCA CGCTTGAGGC CTCTTGGCCT 
CACATACAGT TGGTATATGA ATTCTTCTTG AGATTTTTGG AGAGCCCTGA TTTCCAGCCT 
AGCATTGCAA AACGATACAT TGATCAGAAA TTCGTACAAC AGCTCCTGGA GCTTTTTGAT 
AGTGAAGATC CCAGAGAACG TGACTTCCTG AAGACTGTTC TGCACCGAAT TTATGGGAAA 
TTTCTTGGAT TAAGAGCATT CATCAGAAAA CAAATTAACA ACATTTTCCT CAGGTTTATA 
TATGAAACAG AACATTTCAA TGGTGTTGCT GAACTTCTTG AAATATTAGG AAGTATTATC 
AATGGCTTTG CATTGCCACT GAAAGCAGAA CATAAACAAT TTCTAATGAA GGTTCTTATT 
CCTATGCATA CTGCAAAAGG ATTAGCTTTG TTTCATGCTC AGCTAGCATA TTGTGTTGTA 
CAGTTCCTGG AGAAAGATAC AACACTAACA GAGCCAGTGA TCAGAGGACT GCTGAAATTT 
TGGCCAAAAA CCTGCAGTCA GAAAGAGGTG ATGTTTTTAG GAGAAATTGA AGAAATCTTA 
GATGTCATTG AACCAACACA GTTCAAAAAA ATTGAAGAGC CACTTTTCAA GCAGATATCC 
AAGTGTGTAT CCAGTTCTCA TTTTCAGGTT GCAGAAAGGG CATTGTACTT CTGGAATAAC 
GAATATATTC TTAGTTTGAT TGAGGAGAAC ATTGATAAAA TTCTGCCAAT TATGTTTGCC 
AGTTTGTACA AAATTTCCAA AGAACACTGG AATCCGACCA TTGTAGCACT GGTATACAAT 
GTGCTGAAAA CCCTAATGGA AATGAATGGC AAGCTTTTCG ATGACCTTAC TAGCTCATAC 
AAAGCTGAAA GACAGAGAGA GAAAAAGAAG GAATTGGAAC GTGAAGAATT ATGGAAAAAA 
TTAGAGGAGC TAAAGCTAAA GAAAGCTCTA GAAAAACAGA ATAGTGCTTA CAACATGCAC 
AGTATTCTCA GCAATACAAG TGCCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186685.1
CDS	278..1567
Translation	MIMNATSNEQQELFCQKLQQCCILFDFMDSVSDLKSKEIKRATLNELVEYVSTNRGVIVESAYSDIVKMISANIFRTLPPSDNPDFDPEEDEPTLEASWPHIQLVYEFFLRFLESPDFQPSIAKRYIDQKFVQQLLELFDSEDPRERDFLKTVLHRIYGKFLGLRAFIRKQINNIFLRFIYETEHFNGVAELLEILGSIINGFALPLKAEHKQFLMKVLIPMHTAKGLALFHAQLAYCVVQFLEKDTTLTEPVIRGLLKFWPKTCSQKEVMFLGEIEEILDVIEPTQFKKIEEPLFKQISKCVSSSHFQVAERALYFWNNEYILSLIEENIDKILPIMFASLYKISKEHWNPTIVALVYNVLKTLMEMNGKLFDDLTSSYKAERQREKKKELEREELWKKLEELKLKKALEKQNSAYNMHSILSNTSAE

Target ORF information:

RefSeq Version	NM_001199756.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 2 regulatory subunit B'alpha (PPP2R5A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199756.1

ORF Insert Sequence:

ATGATAATGA ATGCCACTTC AAATGAACAA CAAGAGCTTT TCTGTCAGAA GTTGCAGCAG 
TGTTGTATAC TGTTTGATTT CATGGACTCT GTTTCAGACT TGAAGAGCAA AGAAATTAAA 
AGAGCAACAC TGAATGAACT GGTTGAGTAT GTTTCAACTA ATCGTGGTGT AATTGTTGAA 
TCAGCGTATT CTGATATAGT AAAAATGATC AGTGCTAACA TCTTCCGTAC ACTTCCTCCA 
AGTGATAATC CAGATTTTGA TCCAGAAGAG GATGAACCCA CGCTTGAGGC CTCTTGGCCT 
CACATACAGT TGGTATATGA ATTCTTCTTG AGATTTTTGG AGAGCCCTGA TTTCCAGCCT 
AGCATTGCAA AACGATACAT TGATCAGAAA TTCGTACAAC AGCTCCTGGA GCTTTTTGAT 
AGTGAAGATC CCAGAGAACG TGACTTCCTG AAGACTGTTC TGCACCGAAT TTATGGGAAA 
TTTCTTGGAT TAAGAGCATT CATCAGAAAA CAAATTAACA ACATTTTCCT CAGGTTTATA 
TATGAAACAG AACATTTCAA TGGTGTTGCT GAACTTCTTG AAATATTAGG AAGTATTATC 
AATGGCTTTG CATTGCCACT GAAAGCAGAA CATAAACAAT TTCTAATGAA GGTTCTTATT 
CCTATGCATA CTGCAAAAGG ATTAGCTTTG TTTCATGCTC AGCTAGCATA TTGTGTTGTA 
CAGTTCCTGG AGAAAGATAC AACACTAACA GAGCCAGTGA TCAGAGGACT GCTGAAATTT 
TGGCCAAAAA CCTGCAGTCA GAAAGAGGTG ATGTTTTTAG GAGAAATTGA AGAAATCTTA 
GATGTCATTG AACCAACACA GTTCAAAAAA ATTGAAGAGC CACTTTTCAA GCAGATATCC 
AAGTGTGTAT CCAGTTCTCA TTTTCAGGTT GCAGAAAGGG CATTGTACTT CTGGAATAAC 
GAATATATTC TTAGTTTGAT TGAGGAGAAC ATTGATAAAA TTCTGCCAAT TATGTTTGCC 
AGTTTGTACA AAATTTCCAA AGAACACTGG AATCCGACCA TTGTAGCACT GGTATACAAT 
GTGCTGAAAA CCCTAATGGA AATGAATGGC AAGCTTTTCG ATGACCTTAC TAGCTCATAC 
AAAGCTGAAA GACAGAGAGA GAAAAAGAAG GAATTGGAAC GTGAAGAATT ATGGAAAAAA 
TTAGAGGAGC TAAAGCTAAA GAAAGCTCTA GAAAAACAGA ATAGTGCTTA CAACATGCAC 
AGTATTCTCA GCAATACAAG TGCCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PPP2R5A: A multirole protein phosphatase subunit in regulating cancer development.
Cancer letters414222-229(2018 Feb)
Mao Z,Liu C,Lin X,Sun B,Su C

MicroRNA-218 promotes cisplatin resistance in oral cancer via the PPP2R5A/Wnt signaling pathway.
Oncology reports38(4)2051-2061(2017 Oct)
Zhuang Z,Hu F,Hu J,Wang C,Hou J,Yu Z,Wang TT,Liu X,Huang H

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The PP2A
Journal of cell science130(10)1760-1771(2017 May)
Lee SJ,Rodriguez-Bravo V,Kim H,Datta S,Foley EA

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PPP2R5A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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