TMEM57 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM57
Entrez Gene ID	55219
Full Name	macoilin 1
Synonyms	MACOILIN,TMEM57
General protein information	Preferred Names macoilin 1 Names macoilin transmembrane protein 57
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.11\|1p36.11
Disorder MIM:	610301

mRNA and Protein(s)

mRNA	Protein	Name
NM_001282564.1	NP_001269493.1	macoilin isoform b
NM_018202.6	NP_060672.2	macoilin isoform a
XM_005245931.2	XP_005245988.1	macoilin isoform X1
NM_018202.5	NP_060672.2	macoilin isoform a
XM_011541704.3	XP_011540006.1	macoilin isoform X2
XM_011541704.2	XP_011540006.1	macoilin isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TMEM57	NP_001033738.1
Rattus norvegicus (Norway rat)	Tmem57	NP_001020870.2
Caenorhabditis elegans (roundworm)	maco-1	NP_491902.4
	TMEM57	NP_001033739.1
Canis lupus familiaris (dog)	TMEM57	NP_001033737.1
Danio rerio (zebrafish)	tmem57a	NP_956613.2
Gallus gallus (chicken)	TMEM57	NP_001026066.2
Bos taurus (cattle)	TMEM57	NP_001033736.1
Mus musculus (house mouse)	Tmem57	NP_079658.2
Drosophila melanogaster (fruit fly)	CG30389	NP_001261116.1
Homo sapiens (human)	TMEM57	NP_060672.2
Xenopus tropicalis (tropical clawed frog)	tmem57	NP_001037870.1

Gene Ontology
Bibliography

Related articles in PubMed

Polymorphism of rs873308 near the transmembrane protein 57 gene is associated with serum lipid levels.
Guo T, Yin RX, Lin QZ, Wu J, Shen SW, Sun JQ, Shi GY, Wu JZ, Li H, Wang YM
Bioscience reports34(2)(2014 Apr)

Discovery and refinement of loci associated with lipid levels.
Willer CJ, Schmidt EM, Sengupta S, Peloso GM, Gustafsson S, Kanoni S, Ganna A, Chen J, Buchkovich ML, Mora S, Beckmann JS, Bragg-Gresham JL, Chang HY, Demirkan A, Den Hertog HM, Do R, Donnelly LA, Ehret GB, Esko T, Feitosa MF, Ferreira T, Fischer K, Fontanillas P, Fraser RM, Freitag DF, Gurdasani D, Heikkil? K, Hypp?nen E, Isaacs A, Jackson AU, Johansson ?, Johnson T, Kaakinen M, Kettunen J, Kleber ME, Li X, Luan J, Lyytik?inen LP, Magnusson PKE, Mangino M, Mihailov E, Montasser ME, M?ller-Nurasyid M, Nolte IM, O'Connell JR, Palmer CD, Perola M, Petersen AK, Sanna S, Saxena R, Service SK, Shah S, Shungin D, Sidore C, Song C, Strawbridge RJ, Surakka I, Tanaka T, Teslovich TM, Thorleifsson G, Van den Herik EG, Voight BF, Volcik KA, Waite LL, Wong A, Wu Y, Zhang W, Absher D, Asiki G, Barroso I, Been LF, Bolton JL, Bonnycastle LL, Brambilla P, Burnett MS, Cesana G, Dimitriou M, Doney ASF, D?ring A, Elliott P, Epstein SE, Ingi Eyjolfsson G, Gigante B, Goodarzi MO, Grallert H, Gravito ML, Groves CJ, Hallmans G, Hartikainen AL, Hayward C, Hernandez D, Hicks AA, Holm H, Hung YJ, Illig T, Jones MR, Kaleebu P, Kastelein JJP, Khaw KT, Kim E, Klopp N, Komulainen P, Kumari M, Langenberg C, Lehtim?ki T, Lin SY, Lindstr?m J, Loos RJF, Mach F, McArdle WL, Meisinger C, Mitchell BD, M?ller G, Nagaraja R, Narisu N, Nieminen TVM, Nsubuga RN, Olafsson I, Ong KK, Palotie A, Papamarkou T, Pomilla C, Pouta A, Rader DJ, Reilly MP, Ridker PM, Rivadeneira F, Rudan I, Ruokonen A, Samani N, Scharnagl H, Seeley J, Silander K, Stan??kov? A, Stirrups K, Swift AJ, Tiret L, Uitterlinden AG, van Pelt LJ, Vedantam S, Wainwright N, Wijmenga C, Wild SH, Willemsen G, Wilsgaard T, Wilson JF, Young EH, Zhao JH, Adair LS, Arveiler D, Assimes TL, Bandinelli S, Bennett F, Bochud M, Boehm BO, Boomsma DI, Borecki IB, Bornstein SR, Bovet P, Burnier M, Campbell H, Chakravarti A, Chambers JC, Chen YI, Collins FS, Cooper RS, Danesh J, Dedoussis G, de Faire U, Feranil AB, Ferri?res J, Ferrucci L, Freimer NB, Gieger C, Groop LC, Gudnason V, Gyllensten U, Hamsten A, Harris TB, Hingorani A, Hirschhorn JN, Hofman A, Hovingh GK, Hsiung CA, Humphries SE, Hunt SC, Hveem K, Iribarren C, J?rvelin MR, Jula A, K?h?nen M, Kaprio J, Kes?niemi A, Kivimaki M, Kooner JS, Koudstaal PJ, Krauss RM, Kuh D, Kuusisto J, Kyvik KO, Laakso M, Lakka TA, Lind L, Lindgren CM, Martin NG, M?rz W, McCarthy MI, McKenzie CA, Meneton P, Metspalu A, Moilanen L, Morris AD, Munroe PB, Nj?lstad I, Pedersen NL, Power C, Pramstaller PP, Price JF, Psaty BM, Quertermous T, Rauramaa R, Saleheen D, Salomaa V, Sanghera DK, Saramies J, Schwarz PEH, Sheu WH, Shuldiner AR, Siegbahn A, Spector TD, Stefansson K, Strachan DP, Tayo BO, Tremoli E, Tuomilehto J, Uusitupa M, van Duijn CM, Vollenweider P, Wallentin L, Wareham NJ, Whitfield JB, Wolffenbuttel BHR, Ordovas JM, Boerwinkle E, Palmer CNA, Thorsteinsdottir U, Chasman DI, Rotter JI, Franks PW, Ripatti S, Cupples LA, Sandhu MS, Rich SS, Boehnke M, Deloukas P, Kathiresan S, Mohlke KL, Ingelsson E, Abecasis GR,
Nature genetics45(11)1274-1283(2013 Nov)

Complement receptor 1 gene variants are associated with erythrocyte sedimentation rate.
Kullo IJ, Ding K, Shameer K, McCarty CA, Jarvik GP, Denny JC, Ritchie MD, Ye Z, Crosslin DR, Chisholm RL, Manolio TA, Chute CG
American journal of human genetics89(1)131-8(2011 Jul)

Novel and conserved protein macoilin is required for diverse neuronal functions in Caenorhabditis elegans.
Miyara A, Ohta A, Okochi Y, Tsukada Y, Kuhara A, Mori I
PLoS genetics7(5)e1001384(2011 May)

Macoilin, a conserved nervous system-specific ER membrane protein that regulates neuronal excitability.
Arellano-Carbajal F, Brise?o-Roa L, Couto A, Cheung BH, Labouesse M, de Bono M
PLoS genetics7(3)e1001341(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results indicate that PAK4 confers CDDP resistance via the activation of MEK/ERK and PI3K/Akt pathways. PAK4 and PI3K/Akt pathways can reciprocally activate each other.
Title: Polymorphism of rs873308 near the transmembrane protein 57 gene is associated with serum lipid levels.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Loci influencing lipid levels and coronary heart disease risk in 16 European population cohorts.

The following TMEM57 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM57 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25139	NM_018202.6 Latest version!	Homo sapiens macoilin 1 (MACO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu69188	XM_011541704.3 Latest version!	Homo sapiens macoilin 1 (MACO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OHu25205	NM_001282564.1 Latest version!	Homo sapiens macoilin 1 (MACO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu33576	XM_005245931.2 Latest version!	Homo sapiens macoilin 1 (MACO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu25139	NM_018202.5	Homo sapiens macoilin 1 (MACO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu69188	XM_011541704.2	Homo sapiens transmembrane protein 57 (TMEM57), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM57 ( NM_018202.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060672.2 Homo sapiens macoilin 1 (MACO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25139
Clone ID Related Accession (Same CDS sequence)	NM_018202.6 , NM_018202.5
Accession Version	NM_018202.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	macoilin isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB455940.1, BC032427.1, BX572623.8 and AA827805.1. On Nov 23, 2018 this sequence version replaced NM_018202.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY845015.1, SRR1660805.142087.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374343.5/ ENSP00000363463.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGCGGC GGAACGCCGA CTGCAGTAAG CTCCGCCGCC CCCTAAAGCG GAACCGGATC 
ACCGAGGGCA TTTACGGCAG TACATTTTTA TACCTGAAAT TCCTGGTGGT GTGGGCACTT 
GTCCTCCTAG CAGATTTTGT CCTGGAGTTC AGATTTGAAT ACCTGTGGCC ATTCTGGCTT 
TTCATCAGAA GCGTCTATGA TTCCTTCAGA TACCAGGGAC TGGCCTTCTC AGTATTTTTT 
GTTTGTGTAG CATTCACGTC AAATATAATA TGCCTGCTGT TCATCCCCAT ACAGTGGCTT 
TTTTTTGCTG CTAGCACATA TGTATGGGTT CAGTACGTAT GGCACACAGA AAGGGGAGTG 
TGTTTGCCTA CAGTGTCTCT CTGGATCCTC TTTGTTTATA TTGAAGCAGC CATTAGATTT 
AAAGATCTCA AAAACTTTCA TGTAGACCTT TGTCGTCCAT TTGCTGCTCA CTGTATTGGG 
TACCCTGTGG TAACTTTGGG GTTTGGCTTC AAAAGTTACG TAAGCTACAA AATGCGGTTA 
AGGAAGCAAA AAGAAGTACA AAAAGAGAAC GAGTTTTACA TGCAACTTCT TCAACAAGCT 
CTCCCTCCAG AGCAACAGAT GCTACAGAAG CAAGAAAAAG AGGCCGAGGA AGCAGCCAAA 
GGATTACCTG ATATGGATTC TTCGATCCTT ATACACCACA ATGGAGGTAT CCCAGCCAAC 
AAAAAACTCT CCACAACTTT GCCAGAGATA GAATACCGAG AAAAAGGGAA AGAAAAGGAC 
AAGGATGCCA AAAAACACAA CCTTGGAATA AATAACAACA ATATTCTACA ACCTGTAGAC 
TCTAAAATAC AAGAGATTGA GTATATGGAA AACCATATCA ATAGTAAAAG ATTAAATAAT 
GATCTTGTGG GAAGTACAGA AAATCTCTTG AAAGAGGACT CATGCACTGC TTCCTCAAAA 
AATTACAAAA ATGCCAGTGG AGTTGTGAAC TCTTCACCTC GAAGTCATAG CGCCACAAAT 
GGGAGCATTC CTTCCTCATC TAGTAAAAAT GAGAAGAAGC AGAAATGCAC TAGCAAGAGC 
CCAAGTACAC ACAAGGACTT AATGGAAAAC TGTATTCCTA ATAACCAGCT AAGCAAACCA 
GACGCACTGG TCAGGCTGGA ACAAGACATT AAAAAGTTAA AGGCTGACCT GCAAGCCAGC 
AGACAAGTGG AACAAGAGCT CCGCAGTCAG ATCAGCTCCC TTTCGAGCAC CGAGCGAGGG 
ATCCGCTCAG AAATGGGCCA GCTTCGGCAG GAGAACGAGC TGCTGCAGAA CAAGTTACAT 
AATGCTGTGC AAATGAAGCA AAAAGACAAG CAGAATATCA GCCAGTTGGA GAAAAAGCTA 
AAAGCTGAGC AGGAAGCCCG AAGTTTTGTA GAGAAACAGT TAATGGAAGA GAAAAAGAGG 
AAGAAGTTAG AAGAAGCCAC TGCTGCCCGG GCTGTTGCGT TTGCTGCTGC ATCTAGGGGA 
GAATGCACCG AAACCTTACG GAATCGGATC AGAGAACTAG AAGCAGAGGG CAAGAAGCTC 
ACGATGGACA TGAAGGTGAA AGAAGACCAA ATCAGAGAAC TAGAACTAAA AGTCCAGGAG 
CTTCGGAAAT ATAAGGAAAA TGAGAAGGAC ACTGAGGTGT TAATGTCAGC CCTCTCAGCC 
ATGCAAGACA AAACACAGCA CCTGGAGAAC AGCTTAAGTG CAGAGACGAG AATCAAGCTG 
GACCTGTTCT CCGCACTGGG CGATGCAAAG CGGCAGCTCG AGATTGCCCA AGGACAAATC 
CTTCAGAAAG ATCAGGAAAT CAAGGACCTA AAACAGAAGA TAGCCGAAGT CATGGCCGTC 
ATGCCCAGCA TAACATACAG TGCCGCCACC AGCCCCCTGA GCCCTGTTTC CCCCCACTAC 
TCTTCCAAAT TTGTGGAGAC CAGCCCCTCT GGACTTGACC CCAATGCCTC TGTTTACCAG 
CCCCTGAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060672.2
CDS	203..2197
Translation	MKRRNADCSKLRRPLKRNRITEGIYGSTFLYLKFLVVWALVLLADFVLEFRFEYLWPFWLFIRSVYDSFRYQGLAFSVFFVCVAFTSNIICLLFIPIQWLFFAASTYVWVQYVWHTERGVCLPTVSLWILFVYIEAAIRFKDLKNFHVDLCRPFAAHCIGYPVVTLGFGFKSYVSYKMRLRKQKEVQKENEFYMQLLQQALPPEQQMLQKQEKEAEEAAKGLPDMDSSILIHHNGGIPANKKLSTTLPEIEYREKGKEKDKDAKKHNLGINNNNILQPVDSKIQEIEYMENHINSKRLNNDLVGSTENLLKEDSCTASSKNYKNASGVVNSSPRSHSATNGSIPSSSSKNEKKQKCTSKSPSTHKDLMENCIPNNQLSKPDALVRLEQDIKKLKADLQASRQVEQELRSQISSLSSTERGIRSEMGQLRQENELLQNKLHNAVQMKQKDKQNISQLEKKLKAEQEARSFVEKQLMEEKKRKKLEEATAARAVAFAAASRGECTETLRNRIRELEAEGKKLTMDMKVKEDQIRELELKVQELRKYKENEKDTEVLMSALSAMQDKTQHLENSLSAETRIKLDLFSALGDAKRQLEIAQGQILQKDQEIKDLKQKIAEVMAVMPSITYSAATSPLSPVSPHYSSKFVETSPSGLDPNASVYQPLKK

Target ORF information:

RefSeq Version	NM_018202.6
Organism	Homo sapiens(human)
Definition	Homo sapiens macoilin 1 (MACO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018202.6

ORF Insert Sequence:

ATGAAGCGGC GGAACGCCGA CTGCAGTAAG CTCCGCCGCC CCCTAAAGCG GAACCGGATC 
ACCGAGGGCA TTTACGGCAG TACATTTTTA TACCTGAAAT TCCTGGTGGT GTGGGCACTT 
GTCCTCCTAG CAGATTTTGT CCTGGAGTTC AGATTTGAAT ACCTGTGGCC ATTCTGGCTT 
TTCATCAGAA GCGTCTATGA TTCCTTCAGA TACCAGGGAC TGGCCTTCTC AGTATTTTTT 
GTTTGTGTAG CATTCACGTC AAATATAATA TGCCTGCTGT TCATCCCCAT ACAGTGGCTT 
TTTTTTGCTG CTAGCACATA TGTATGGGTT CAGTACGTAT GGCACACAGA AAGGGGAGTG 
TGTTTGCCTA CAGTGTCTCT CTGGATCCTC TTTGTTTATA TTGAAGCAGC CATTAGATTT 
AAAGATCTCA AAAACTTTCA TGTAGACCTT TGTCGTCCAT TTGCTGCTCA CTGTATTGGG 
TACCCTGTGG TAACTTTGGG GTTTGGCTTC AAAAGTTACG TAAGCTACAA AATGCGGTTA 
AGGAAGCAAA AAGAAGTACA AAAAGAGAAC GAGTTTTACA TGCAACTTCT TCAACAAGCT 
CTCCCTCCAG AGCAACAGAT GCTACAGAAG CAAGAAAAAG AGGCCGAGGA AGCAGCCAAA 
GGATTACCTG ATATGGATTC TTCGATCCTT ATACACCACA ATGGAGGTAT CCCAGCCAAC 
AAAAAACTCT CCACAACTTT GCCAGAGATA GAATACCGAG AAAAAGGGAA AGAAAAGGAC 
AAGGATGCCA AAAAACACAA CCTTGGAATA AATAACAACA ATATTCTACA ACCTGTAGAC 
TCTAAAATAC AAGAGATTGA GTATATGGAA AACCATATCA ATAGTAAAAG ATTAAATAAT 
GATCTTGTGG GAAGTACAGA AAATCTCTTG AAAGAGGACT CATGCACTGC TTCCTCAAAA 
AATTACAAAA ATGCCAGTGG AGTTGTGAAC TCTTCACCTC GAAGTCATAG CGCCACAAAT 
GGGAGCATTC CTTCCTCATC TAGTAAAAAT GAGAAGAAGC AGAAATGCAC TAGCAAGAGC 
CCAAGTACAC ACAAGGACTT AATGGAAAAC TGTATTCCTA ATAACCAGCT AAGCAAACCA 
GACGCACTGG TCAGGCTGGA ACAAGACATT AAAAAGTTAA AGGCTGACCT GCAAGCCAGC 
AGACAAGTGG AACAAGAGCT CCGCAGTCAG ATCAGCTCCC TTTCGAGCAC CGAGCGAGGG 
ATCCGCTCAG AAATGGGCCA GCTTCGGCAG GAGAACGAGC TGCTGCAGAA CAAGTTACAT 
AATGCTGTGC AAATGAAGCA AAAAGACAAG CAGAATATCA GCCAGTTGGA GAAAAAGCTA 
AAAGCTGAGC AGGAAGCCCG AAGTTTTGTA GAGAAACAGT TAATGGAAGA GAAAAAGAGG 
AAGAAGTTAG AAGAAGCCAC TGCTGCCCGG GCTGTTGCGT TTGCTGCTGC ATCTAGGGGA 
GAATGCACCG AAACCTTACG GAATCGGATC AGAGAACTAG AAGCAGAGGG CAAGAAGCTC 
ACGATGGACA TGAAGGTGAA AGAAGACCAA ATCAGAGAAC TAGAACTAAA AGTCCAGGAG 
CTTCGGAAAT ATAAGGAAAA TGAGAAGGAC ACTGAGGTGT TAATGTCAGC CCTCTCAGCC 
ATGCAAGACA AAACACAGCA CCTGGAGAAC AGCTTAAGTG CAGAGACGAG AATCAAGCTG 
GACCTGTTCT CCGCACTGGG CGATGCAAAG CGGCAGCTCG AGATTGCCCA AGGACAAATC 
CTTCAGAAAG ATCAGGAAAT CAAGGACCTA AAACAGAAGA TAGCCGAAGT CATGGCCGTC 
ATGCCCAGCA TAACATACAG TGCCGCCACC AGCCCCCTGA GCCCTGTTTC CCCCCACTAC 
TCTTCCAAAT TTGTGGAGAC CAGCCCCTCT GGACTTGACC CCAATGCCTC TGTTTACCAG 
CCCCTGAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM57 ( NM_018202.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060672.2 Homo sapiens macoilin 1 (MACO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25139
Clone ID Related Accession (Same CDS sequence)	NM_018202.6 , NM_018202.5
Accession Version	NM_018202.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-28
Organism	Homo sapiens(human)
Product	macoilin isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB455940.1, BC032427.1, BX572623.8 and AA827805.1. On Sep 13, 2013 this sequence version replaced NM_018202.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY845015.1, SRR1660805.142087.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCGGC GGAACGCCGA CTGCAGTAAG CTCCGCCGCC CCCTAAAGCG GAACCGGATC 
ACCGAGGGCA TTTACGGCAG TACATTTTTA TACCTGAAAT TCCTGGTGGT GTGGGCACTT 
GTCCTCCTAG CAGATTTTGT CCTGGAGTTC AGATTTGAAT ACCTGTGGCC ATTCTGGCTT 
TTCATCAGAA GCGTCTATGA TTCCTTCAGA TACCAGGGAC TGGCCTTCTC AGTATTTTTT 
GTTTGTGTAG CATTCACGTC AAATATAATA TGCCTGCTGT TCATCCCCAT ACAGTGGCTT 
TTTTTTGCTG CTAGCACATA TGTATGGGTT CAGTACGTAT GGCACACAGA AAGGGGAGTG 
TGTTTGCCTA CAGTGTCTCT CTGGATCCTC TTTGTTTATA TTGAAGCAGC CATTAGATTT 
AAAGATCTCA AAAACTTTCA TGTAGACCTT TGTCGTCCAT TTGCTGCTCA CTGTATTGGG 
TACCCTGTGG TAACTTTGGG GTTTGGCTTC AAAAGTTACG TAAGCTACAA AATGCGGTTA 
AGGAAGCAAA AAGAAGTACA AAAAGAGAAC GAGTTTTACA TGCAACTTCT TCAACAAGCT 
CTCCCTCCAG AGCAACAGAT GCTACAGAAG CAAGAAAAAG AGGCCGAGGA AGCAGCCAAA 
GGATTACCTG ATATGGATTC TTCGATCCTT ATACACCACA ATGGAGGTAT CCCAGCCAAC 
AAAAAACTCT CCACAACTTT GCCAGAGATA GAATACCGAG AAAAAGGGAA AGAAAAGGAC 
AAGGATGCCA AAAAACACAA CCTTGGAATA AATAACAACA ATATTCTACA ACCTGTAGAC 
TCTAAAATAC AAGAGATTGA GTATATGGAA AACCATATCA ATAGTAAAAG ATTAAATAAT 
GATCTTGTGG GAAGTACAGA AAATCTCTTG AAAGAGGACT CATGCACTGC TTCCTCAAAA 
AATTACAAAA ATGCCAGTGG AGTTGTGAAC TCTTCACCTC GAAGTCATAG CGCCACAAAT 
GGGAGCATTC CTTCCTCATC TAGTAAAAAT GAGAAGAAGC AGAAATGCAC TAGCAAGAGC 
CCAAGTACAC ACAAGGACTT AATGGAAAAC TGTATTCCTA ATAACCAGCT AAGCAAACCA 
GACGCACTGG TCAGGCTGGA ACAAGACATT AAAAAGTTAA AGGCTGACCT GCAAGCCAGC 
AGACAAGTGG AACAAGAGCT CCGCAGTCAG ATCAGCTCCC TTTCGAGCAC CGAGCGAGGG 
ATCCGCTCAG AAATGGGCCA GCTTCGGCAG GAGAACGAGC TGCTGCAGAA CAAGTTACAT 
AATGCTGTGC AAATGAAGCA AAAAGACAAG CAGAATATCA GCCAGTTGGA GAAAAAGCTA 
AAAGCTGAGC AGGAAGCCCG AAGTTTTGTA GAGAAACAGT TAATGGAAGA GAAAAAGAGG 
AAGAAGTTAG AAGAAGCCAC TGCTGCCCGG GCTGTTGCGT TTGCTGCTGC ATCTAGGGGA 
GAATGCACCG AAACCTTACG GAATCGGATC AGAGAACTAG AAGCAGAGGG CAAGAAGCTC 
ACGATGGACA TGAAGGTGAA AGAAGACCAA ATCAGAGAAC TAGAACTAAA AGTCCAGGAG 
CTTCGGAAAT ATAAGGAAAA TGAGAAGGAC ACTGAGGTGT TAATGTCAGC CCTCTCAGCC 
ATGCAAGACA AAACACAGCA CCTGGAGAAC AGCTTAAGTG CAGAGACGAG AATCAAGCTG 
GACCTGTTCT CCGCACTGGG CGATGCAAAG CGGCAGCTCG AGATTGCCCA AGGACAAATC 
CTTCAGAAAG ATCAGGAAAT CAAGGACCTA AAACAGAAGA TAGCCGAAGT CATGGCCGTC 
ATGCCCAGCA TAACATACAG TGCCGCCACC AGCCCCCTGA GCCCTGTTTC CCCCCACTAC 
TCTTCCAAAT TTGTGGAGAC CAGCCCCTCT GGACTTGACC CCAATGCCTC TGTTTACCAG 
CCCCTGAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060672.2
CDS	242..2236
Translation	MKRRNADCSKLRRPLKRNRITEGIYGSTFLYLKFLVVWALVLLADFVLEFRFEYLWPFWLFIRSVYDSFRYQGLAFSVFFVCVAFTSNIICLLFIPIQWLFFAASTYVWVQYVWHTERGVCLPTVSLWILFVYIEAAIRFKDLKNFHVDLCRPFAAHCIGYPVVTLGFGFKSYVSYKMRLRKQKEVQKENEFYMQLLQQALPPEQQMLQKQEKEAEEAAKGLPDMDSSILIHHNGGIPANKKLSTTLPEIEYREKGKEKDKDAKKHNLGINNNNILQPVDSKIQEIEYMENHINSKRLNNDLVGSTENLLKEDSCTASSKNYKNASGVVNSSPRSHSATNGSIPSSSSKNEKKQKCTSKSPSTHKDLMENCIPNNQLSKPDALVRLEQDIKKLKADLQASRQVEQELRSQISSLSSTERGIRSEMGQLRQENELLQNKLHNAVQMKQKDKQNISQLEKKLKAEQEARSFVEKQLMEEKKRKKLEEATAARAVAFAAASRGECTETLRNRIRELEAEGKKLTMDMKVKEDQIRELELKVQELRKYKENEKDTEVLMSALSAMQDKTQHLENSLSAETRIKLDLFSALGDAKRQLEIAQGQILQKDQEIKDLKQKIAEVMAVMPSITYSAATSPLSPVSPHYSSKFVETSPSGLDPNASVYQPLKK

Target ORF information:

RefSeq Version	NM_018202.5
Organism	Homo sapiens(human)
Definition	Homo sapiens macoilin 1 (MACO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018202.5

ORF Insert Sequence:

ATGAAGCGGC GGAACGCCGA CTGCAGTAAG CTCCGCCGCC CCCTAAAGCG GAACCGGATC 
ACCGAGGGCA TTTACGGCAG TACATTTTTA TACCTGAAAT TCCTGGTGGT GTGGGCACTT 
GTCCTCCTAG CAGATTTTGT CCTGGAGTTC AGATTTGAAT ACCTGTGGCC ATTCTGGCTT 
TTCATCAGAA GCGTCTATGA TTCCTTCAGA TACCAGGGAC TGGCCTTCTC AGTATTTTTT 
GTTTGTGTAG CATTCACGTC AAATATAATA TGCCTGCTGT TCATCCCCAT ACAGTGGCTT 
TTTTTTGCTG CTAGCACATA TGTATGGGTT CAGTACGTAT GGCACACAGA AAGGGGAGTG 
TGTTTGCCTA CAGTGTCTCT CTGGATCCTC TTTGTTTATA TTGAAGCAGC CATTAGATTT 
AAAGATCTCA AAAACTTTCA TGTAGACCTT TGTCGTCCAT TTGCTGCTCA CTGTATTGGG 
TACCCTGTGG TAACTTTGGG GTTTGGCTTC AAAAGTTACG TAAGCTACAA AATGCGGTTA 
AGGAAGCAAA AAGAAGTACA AAAAGAGAAC GAGTTTTACA TGCAACTTCT TCAACAAGCT 
CTCCCTCCAG AGCAACAGAT GCTACAGAAG CAAGAAAAAG AGGCCGAGGA AGCAGCCAAA 
GGATTACCTG ATATGGATTC TTCGATCCTT ATACACCACA ATGGAGGTAT CCCAGCCAAC 
AAAAAACTCT CCACAACTTT GCCAGAGATA GAATACCGAG AAAAAGGGAA AGAAAAGGAC 
AAGGATGCCA AAAAACACAA CCTTGGAATA AATAACAACA ATATTCTACA ACCTGTAGAC 
TCTAAAATAC AAGAGATTGA GTATATGGAA AACCATATCA ATAGTAAAAG ATTAAATAAT 
GATCTTGTGG GAAGTACAGA AAATCTCTTG AAAGAGGACT CATGCACTGC TTCCTCAAAA 
AATTACAAAA ATGCCAGTGG AGTTGTGAAC TCTTCACCTC GAAGTCATAG CGCCACAAAT 
GGGAGCATTC CTTCCTCATC TAGTAAAAAT GAGAAGAAGC AGAAATGCAC TAGCAAGAGC 
CCAAGTACAC ACAAGGACTT AATGGAAAAC TGTATTCCTA ATAACCAGCT AAGCAAACCA 
GACGCACTGG TCAGGCTGGA ACAAGACATT AAAAAGTTAA AGGCTGACCT GCAAGCCAGC 
AGACAAGTGG AACAAGAGCT CCGCAGTCAG ATCAGCTCCC TTTCGAGCAC CGAGCGAGGG 
ATCCGCTCAG AAATGGGCCA GCTTCGGCAG GAGAACGAGC TGCTGCAGAA CAAGTTACAT 
AATGCTGTGC AAATGAAGCA AAAAGACAAG CAGAATATCA GCCAGTTGGA GAAAAAGCTA 
AAAGCTGAGC AGGAAGCCCG AAGTTTTGTA GAGAAACAGT TAATGGAAGA GAAAAAGAGG 
AAGAAGTTAG AAGAAGCCAC TGCTGCCCGG GCTGTTGCGT TTGCTGCTGC ATCTAGGGGA 
GAATGCACCG AAACCTTACG GAATCGGATC AGAGAACTAG AAGCAGAGGG CAAGAAGCTC 
ACGATGGACA TGAAGGTGAA AGAAGACCAA ATCAGAGAAC TAGAACTAAA AGTCCAGGAG 
CTTCGGAAAT ATAAGGAAAA TGAGAAGGAC ACTGAGGTGT TAATGTCAGC CCTCTCAGCC 
ATGCAAGACA AAACACAGCA CCTGGAGAAC AGCTTAAGTG CAGAGACGAG AATCAAGCTG 
GACCTGTTCT CCGCACTGGG CGATGCAAAG CGGCAGCTCG AGATTGCCCA AGGACAAATC 
CTTCAGAAAG ATCAGGAAAT CAAGGACCTA AAACAGAAGA TAGCCGAAGT CATGGCCGTC 
ATGCCCAGCA TAACATACAG TGCCGCCACC AGCCCCCTGA GCCCTGTTTC CCCCCACTAC 
TCTTCCAAAT TTGTGGAGAC CAGCCCCTCT GGACTTGACC CCAATGCCTC TGTTTACCAG 
CCCCTGAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM57 ( XM_011541704.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540006.1 Homo sapiens macoilin 1 (MACO1), transcript variant X2, mRNA.

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CloneID	OHu69188
Clone ID Related Accession (Same CDS sequence)	XM_011541704.3 , XM_011541704.2
Accession Version	XM_011541704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	macoilin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011541704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTTAA GGAAGCAAAA AGAAGTACAA AAAGAGAACG AGTTTTACAT GCAACTTCTT 
CAACAAGCTC TCCCTCCAGA GCAACAGATG CTACAGAAGC AAGAAAAAGA GGCCGAGGAA 
GCAGCCAAAG GATTACCTGA TATGGATTCT TCGATCCTTA TACACCACAA TGGAGGTATC 
CCAGCCAACA AAAAACTCTC CACAACTTTG CCAGAGATAG AATACCGAGA AAAAGGGAAA 
GAAAAGGACA AGGATGCCAA AAAACACAAC CTTGGAATAA ATAACAACAA TATTCTACAA 
CCTGTAGACT CTAAAATACA AGAGATTGAG TATATGGAAA ACCATATCAA TAGTAAAAGA 
TTAAATAATG ATCTTGTGGG AAGTACAGAA AATCTCTTGA AAGAGGACTC ATGCACTGCT 
TCCTCAAAAA ATTACAAAAA TGCCAGTGGA GTTGTGAACT CTTCACCTCG AAGTCATAGC 
GCCACAAATG GGAGCATTCC TTCCTCATCT AGTAAAAATG AGAAGAAGCA GAAATGCACT 
AGCAAGAGCC CAAGTACACA CAAGGACTTA ATGGAAAACT GTATTCCTAA TAACCAGCTA 
AGCAAACCAG ACGCACTGGT CAGGCTGGAA CAAGACATTA AAAAGTTAAA GGCTGACCTG 
CAAGCCAGCA GACAAGTGGA ACAAGAGCTC CGCAGTCAGA TCAGCTCCCT TTCGAGCACC 
GAGCGAGGGA TCCGCTCAGA AATGGGCCAG CTTCGGCAGG AGAACGAGCT GCTGCAGAAC 
AAGTTACATA ATGCTGTGCA AATGAAGCAA AAAGACAAGC AGAATATCAG CCAGTTGGAG 
AAAAAGCTAA AAGCTGAGCA GGAAGCCCGA AGTTTTGTAG AGAAACAGTT AATGGAAGAG 
AAAAAGAGGA AGAAGTTAGA AGAAGCCACT GCTGCCCGGG CTGTTGCGTT TGCTGCTGCA 
TCTAGGGGAG AATGCACCGA AACCTTACGG AATCGGATCA GAGAACTAGA AGCAGAGGGC 
AAGAAGCTCA CGATGGACAT GAAGGTGAAA GAAGACCAAA TCAGAGAACT AGAACTAAAA 
GTCCAGGAGC TTCGGAAATA TAAGGAAAAT GAGAAGGACA CTGAGGTGTT AATGTCAGCC 
CTCTCAGCCA TGCAAGACAA AACACAGCAC CTGGAGAACA GCTTAAGTGC AGAGACGAGA 
ATCAAGCTGG ACCTGTTCTC CGCACTGGGC GATGCAAAGC GGCAGCTCGA GATTGCCCAA 
GGACAAATCC TTCAGAAAGA TCAGGAAATC AAGGACCTAA AACAGAAGAT AGCCGAAGTC 
ATGGCCGTCA TGCCCAGCAT AACATACAGT GCCGCCACCA GCCCCCTGAG CCCTGTTTCC 
CCCCACTACT CTTCCAAATT TGTGGAGACC AGCCCCTCTG GACTTGACCC CAATGCCTCT 
GTTTACCAGC CCCTGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540006.1
CDS	807..2270
Translation	MRLRKQKEVQKENEFYMQLLQQALPPEQQMLQKQEKEAEEAAKGLPDMDSSILIHHNGGIPANKKLSTTLPEIEYREKGKEKDKDAKKHNLGINNNNILQPVDSKIQEIEYMENHINSKRLNNDLVGSTENLLKEDSCTASSKNYKNASGVVNSSPRSHSATNGSIPSSSSKNEKKQKCTSKSPSTHKDLMENCIPNNQLSKPDALVRLEQDIKKLKADLQASRQVEQELRSQISSLSSTERGIRSEMGQLRQENELLQNKLHNAVQMKQKDKQNISQLEKKLKAEQEARSFVEKQLMEEKKRKKLEEATAARAVAFAAASRGECTETLRNRIRELEAEGKKLTMDMKVKEDQIRELELKVQELRKYKENEKDTEVLMSALSAMQDKTQHLENSLSAETRIKLDLFSALGDAKRQLEIAQGQILQKDQEIKDLKQKIAEVMAVMPSITYSAATSPLSPVSPHYSSKFVETSPSGLDPNASVYQPLKK

Target ORF information:

RefSeq Version	XM_011541704.3
Organism	Homo sapiens(human)
Definition	Homo sapiens macoilin 1 (MACO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541704.3

ORF Insert Sequence:

ATGCGGTTAA GGAAGCAAAA AGAAGTACAA AAAGAGAACG AGTTTTACAT GCAACTTCTT 
CAACAAGCTC TCCCTCCAGA GCAACAGATG CTACAGAAGC AAGAAAAAGA GGCCGAGGAA 
GCAGCCAAAG GATTACCTGA TATGGATTCT TCGATCCTTA TACACCACAA TGGAGGTATC 
CCAGCCAACA AAAAACTCTC CACAACTTTG CCAGAGATAG AATACCGAGA AAAAGGGAAA 
GAAAAGGACA AGGATGCCAA AAAACACAAC CTTGGAATAA ATAACAACAA TATTCTACAA 
CCTGTAGACT CTAAAATACA AGAGATTGAG TATATGGAAA ACCATATCAA TAGTAAAAGA 
TTAAATAATG ATCTTGTGGG AAGTACAGAA AATCTCTTGA AAGAGGACTC ATGCACTGCT 
TCCTCAAAAA ATTACAAAAA TGCCAGTGGA GTTGTGAACT CTTCACCTCG AAGTCATAGC 
GCCACAAATG GGAGCATTCC TTCCTCATCT AGTAAAAATG AGAAGAAGCA GAAATGCACT 
AGCAAGAGCC CAAGTACACA CAAGGACTTA ATGGAAAACT GTATTCCTAA TAACCAGCTA 
AGCAAACCAG ACGCACTGGT CAGGCTGGAA CAAGACATTA AAAAGTTAAA GGCTGACCTG 
CAAGCCAGCA GACAAGTGGA ACAAGAGCTC CGCAGTCAGA TCAGCTCCCT TTCGAGCACC 
GAGCGAGGGA TCCGCTCAGA AATGGGCCAG CTTCGGCAGG AGAACGAGCT GCTGCAGAAC 
AAGTTACATA ATGCTGTGCA AATGAAGCAA AAAGACAAGC AGAATATCAG CCAGTTGGAG 
AAAAAGCTAA AAGCTGAGCA GGAAGCCCGA AGTTTTGTAG AGAAACAGTT AATGGAAGAG 
AAAAAGAGGA AGAAGTTAGA AGAAGCCACT GCTGCCCGGG CTGTTGCGTT TGCTGCTGCA 
TCTAGGGGAG AATGCACCGA AACCTTACGG AATCGGATCA GAGAACTAGA AGCAGAGGGC 
AAGAAGCTCA CGATGGACAT GAAGGTGAAA GAAGACCAAA TCAGAGAACT AGAACTAAAA 
GTCCAGGAGC TTCGGAAATA TAAGGAAAAT GAGAAGGACA CTGAGGTGTT AATGTCAGCC 
CTCTCAGCCA TGCAAGACAA AACACAGCAC CTGGAGAACA GCTTAAGTGC AGAGACGAGA 
ATCAAGCTGG ACCTGTTCTC CGCACTGGGC GATGCAAAGC GGCAGCTCGA GATTGCCCAA 
GGACAAATCC TTCAGAAAGA TCAGGAAATC AAGGACCTAA AACAGAAGAT AGCCGAAGTC 
ATGGCCGTCA TGCCCAGCAT AACATACAGT GCCGCCACCA GCCCCCTGAG CCCTGTTTCC 
CCCCACTACT CTTCCAAATT TGTGGAGACC AGCCCCTCTG GACTTGACCC CAATGCCTCT 
GTTTACCAGC CCCTGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM57 ( XM_011541704.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011540006.1 Homo sapiens transmembrane protein 57 (TMEM57), transcript variant X2, mRNA.

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CloneID	OHu69188
Clone ID Related Accession (Same CDS sequence)	XM_011541704.3 , XM_011541704.2
Accession Version	XM_011541704.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	macoilin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTTAA GGAAGCAAAA AGAAGTACAA AAAGAGAACG AGTTTTACAT GCAACTTCTT 
CAACAAGCTC TCCCTCCAGA GCAACAGATG CTACAGAAGC AAGAAAAAGA GGCCGAGGAA 
GCAGCCAAAG GATTACCTGA TATGGATTCT TCGATCCTTA TACACCACAA TGGAGGTATC 
CCAGCCAACA AAAAACTCTC CACAACTTTG CCAGAGATAG AATACCGAGA AAAAGGGAAA 
GAAAAGGACA AGGATGCCAA AAAACACAAC CTTGGAATAA ATAACAACAA TATTCTACAA 
CCTGTAGACT CTAAAATACA AGAGATTGAG TATATGGAAA ACCATATCAA TAGTAAAAGA 
TTAAATAATG ATCTTGTGGG AAGTACAGAA AATCTCTTGA AAGAGGACTC ATGCACTGCT 
TCCTCAAAAA ATTACAAAAA TGCCAGTGGA GTTGTGAACT CTTCACCTCG AAGTCATAGC 
GCCACAAATG GGAGCATTCC TTCCTCATCT AGTAAAAATG AGAAGAAGCA GAAATGCACT 
AGCAAGAGCC CAAGTACACA CAAGGACTTA ATGGAAAACT GTATTCCTAA TAACCAGCTA 
AGCAAACCAG ACGCACTGGT CAGGCTGGAA CAAGACATTA AAAAGTTAAA GGCTGACCTG 
CAAGCCAGCA GACAAGTGGA ACAAGAGCTC CGCAGTCAGA TCAGCTCCCT TTCGAGCACC 
GAGCGAGGGA TCCGCTCAGA AATGGGCCAG CTTCGGCAGG AGAACGAGCT GCTGCAGAAC 
AAGTTACATA ATGCTGTGCA AATGAAGCAA AAAGACAAGC AGAATATCAG CCAGTTGGAG 
AAAAAGCTAA AAGCTGAGCA GGAAGCCCGA AGTTTTGTAG AGAAACAGTT AATGGAAGAG 
AAAAAGAGGA AGAAGTTAGA AGAAGCCACT GCTGCCCGGG CTGTTGCGTT TGCTGCTGCA 
TCTAGGGGAG AATGCACCGA AACCTTACGG AATCGGATCA GAGAACTAGA AGCAGAGGGC 
AAGAAGCTCA CGATGGACAT GAAGGTGAAA GAAGACCAAA TCAGAGAACT AGAACTAAAA 
GTCCAGGAGC TTCGGAAATA TAAGGAAAAT GAGAAGGACA CTGAGGTGTT AATGTCAGCC 
CTCTCAGCCA TGCAAGACAA AACACAGCAC CTGGAGAACA GCTTAAGTGC AGAGACGAGA 
ATCAAGCTGG ACCTGTTCTC CGCACTGGGC GATGCAAAGC GGCAGCTCGA GATTGCCCAA 
GGACAAATCC TTCAGAAAGA TCAGGAAATC AAGGACCTAA AACAGAAGAT AGCCGAAGTC 
ATGGCCGTCA TGCCCAGCAT AACATACAGT GCCGCCACCA GCCCCCTGAG CCCTGTTTCC 
CCCCACTACT CTTCCAAATT TGTGGAGACC AGCCCCTCTG GACTTGACCC CAATGCCTCT 
GTTTACCAGC CCCTGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011540006.1
CDS	807..2270
Translation	MRLRKQKEVQKENEFYMQLLQQALPPEQQMLQKQEKEAEEAAKGLPDMDSSILIHHNGGIPANKKLSTTLPEIEYREKGKEKDKDAKKHNLGINNNNILQPVDSKIQEIEYMENHINSKRLNNDLVGSTENLLKEDSCTASSKNYKNASGVVNSSPRSHSATNGSIPSSSSKNEKKQKCTSKSPSTHKDLMENCIPNNQLSKPDALVRLEQDIKKLKADLQASRQVEQELRSQISSLSSTERGIRSEMGQLRQENELLQNKLHNAVQMKQKDKQNISQLEKKLKAEQEARSFVEKQLMEEKKRKKLEEATAARAVAFAAASRGECTETLRNRIRELEAEGKKLTMDMKVKEDQIRELELKVQELRKYKENEKDTEVLMSALSAMQDKTQHLENSLSAETRIKLDLFSALGDAKRQLEIAQGQILQKDQEIKDLKQKIAEVMAVMPSITYSAATSPLSPVSPHYSSKFVETSPSGLDPNASVYQPLKK

Target ORF information:

RefSeq Version	XM_011541704.2
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein 57 (TMEM57), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541704.2

ORF Insert Sequence:

ATGCGGTTAA GGAAGCAAAA AGAAGTACAA AAAGAGAACG AGTTTTACAT GCAACTTCTT 
CAACAAGCTC TCCCTCCAGA GCAACAGATG CTACAGAAGC AAGAAAAAGA GGCCGAGGAA 
GCAGCCAAAG GATTACCTGA TATGGATTCT TCGATCCTTA TACACCACAA TGGAGGTATC 
CCAGCCAACA AAAAACTCTC CACAACTTTG CCAGAGATAG AATACCGAGA AAAAGGGAAA 
GAAAAGGACA AGGATGCCAA AAAACACAAC CTTGGAATAA ATAACAACAA TATTCTACAA 
CCTGTAGACT CTAAAATACA AGAGATTGAG TATATGGAAA ACCATATCAA TAGTAAAAGA 
TTAAATAATG ATCTTGTGGG AAGTACAGAA AATCTCTTGA AAGAGGACTC ATGCACTGCT 
TCCTCAAAAA ATTACAAAAA TGCCAGTGGA GTTGTGAACT CTTCACCTCG AAGTCATAGC 
GCCACAAATG GGAGCATTCC TTCCTCATCT AGTAAAAATG AGAAGAAGCA GAAATGCACT 
AGCAAGAGCC CAAGTACACA CAAGGACTTA ATGGAAAACT GTATTCCTAA TAACCAGCTA 
AGCAAACCAG ACGCACTGGT CAGGCTGGAA CAAGACATTA AAAAGTTAAA GGCTGACCTG 
CAAGCCAGCA GACAAGTGGA ACAAGAGCTC CGCAGTCAGA TCAGCTCCCT TTCGAGCACC 
GAGCGAGGGA TCCGCTCAGA AATGGGCCAG CTTCGGCAGG AGAACGAGCT GCTGCAGAAC 
AAGTTACATA ATGCTGTGCA AATGAAGCAA AAAGACAAGC AGAATATCAG CCAGTTGGAG 
AAAAAGCTAA AAGCTGAGCA GGAAGCCCGA AGTTTTGTAG AGAAACAGTT AATGGAAGAG 
AAAAAGAGGA AGAAGTTAGA AGAAGCCACT GCTGCCCGGG CTGTTGCGTT TGCTGCTGCA 
TCTAGGGGAG AATGCACCGA AACCTTACGG AATCGGATCA GAGAACTAGA AGCAGAGGGC 
AAGAAGCTCA CGATGGACAT GAAGGTGAAA GAAGACCAAA TCAGAGAACT AGAACTAAAA 
GTCCAGGAGC TTCGGAAATA TAAGGAAAAT GAGAAGGACA CTGAGGTGTT AATGTCAGCC 
CTCTCAGCCA TGCAAGACAA AACACAGCAC CTGGAGAACA GCTTAAGTGC AGAGACGAGA 
ATCAAGCTGG ACCTGTTCTC CGCACTGGGC GATGCAAAGC GGCAGCTCGA GATTGCCCAA 
GGACAAATCC TTCAGAAAGA TCAGGAAATC AAGGACCTAA AACAGAAGAT AGCCGAAGTC 
ATGGCCGTCA TGCCCAGCAT AACATACAGT GCCGCCACCA GCCCCCTGAG CCCTGTTTCC 
CCCCACTACT CTTCCAAATT TGTGGAGACC AGCCCCTCTG GACTTGACCC CAATGCCTCT 
GTTTACCAGC CCCTGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM57 ( NM_001282564.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269493.1 Homo sapiens macoilin 1 (MACO1), transcript variant 2, mRNA.

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CloneID	OHu25205
Clone ID Related Accession (Same CDS sequence)	NM_001282564.1
Accession Version	NM_001282564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	macoilin isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB455940.1, AY845035.1, BX572623.8 and AA827805.1. On Sep 13, 2013 this sequence version replaced XM_005245932.1. Transcript Variant: This variant (2) lacks two internal exons, resulting in the loss of an in-frame segment in the central coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY845035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCGGC GGAACGCCGA CTGCAGTAAG CTCCGCCGCC CCCTAAAGCG GAACCGGATC 
ACCGAGGGCA TTTACGGCAG TACATTTTTA TACCTGAAAT TCCTGGTGGT GTGGGCACTT 
GTCCTCCTAG CAGATTTTGT CCTGGAGTTC AGATTTGAAT ACCTGTGGCC ATTCTGGCTT 
TTCATCAGAA GCGTCTATGA TTCCTTCAGA TACCAGGGAC TGGCCTTCTC AGTATTTTTT 
GTTTGTGTAG CATTCACGTC AAATATAATA TGCCTGCTGT TCATCCCCAT ACAGTGGCTT 
TTTTTTGCTG CTAGCACATA TGTATGGGTT CAGTACGTAT GGCACACAGA AAGGGGAGTG 
TGTTTGCCTA CAGTGTCTCT CTGGATCCTC TTTGTTTATA TTGAAGCAGC CATTAGATTT 
AAAGATCTCA AAAACTTTCA TGTAGACCTT TGTCGTCCAT TTGCTGCTCA CTGGCTGGAA 
CAAGACATTA AAAAGTTAAA GGCTGACCTG CAAGCCAGCA GACAAGTGGA ACAAGAGCTC 
CGCAGTCAGA TCAGCTCCCT TTCGAGCACC GAGCGAGGGA TCCGCTCAGA AATGGGCCAG 
CTTCGGCAGG AGAACGAGCT GCTGCAGAAC AAGTTACATA ATGCTGTGCA AATGAAGCAA 
AAAGACAAGC AGAATATCAG CCAGTTGGAG AAAAAGCTAA AAGCTGAGCA GGAAGCCCGA 
AGTTTTGTAG AGAAACAGTT AATGGAAGAG AAAAAGAGGA AGAAGTTAGA AGAAGCCACT 
GCTGCCCGGG CTGTTGCGTT TGCTGCTGCA TCTAGGGGAG AATGCACCGA AACCTTACGG 
AATCGGATCA GAGAACTAGA AGCAGAGGGC AAGAAGCTCA CGATGGACAT GAAGGTGAAA 
GAAGACCAAA TCAGAGAACT AGAACTAAAA GTCCAGGAGC TTCGGAAATA TAAGGAAAAT 
GAGAAGGACA CTGAGGTGTT AATGTCAGCC CTCTCAGCCA TGCAAGACAA AACACAGCAC 
CTGGAGAACA GCTTAAGTGC AGAGACGAGA ATCAAGCTGG ACCTGTTCTC CGCACTGGGC 
GATGCAAAGC GGCAGCTCGA GATTGCCCAA GGACAAATCC TTCAGAAAGA TCAGGAAATC 
AAGGACCTAA AACAGAAGAT AGCCGAAGTC ATGGCCGTCA TGCCCAGCAT AACATACAGT 
GCCGCCACCA GCCCCCTGAG CCCTGTTTCC CCCCACTACT CTTCCAAATT TGTGGAGACC 
AGCCCCTCTG GACTTGACCC CAATGCCTCT GTTTACCAGC CCCTGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269493.1
CDS	242..1555
Translation	MKRRNADCSKLRRPLKRNRITEGIYGSTFLYLKFLVVWALVLLADFVLEFRFEYLWPFWLFIRSVYDSFRYQGLAFSVFFVCVAFTSNIICLLFIPIQWLFFAASTYVWVQYVWHTERGVCLPTVSLWILFVYIEAAIRFKDLKNFHVDLCRPFAAHWLEQDIKKLKADLQASRQVEQELRSQISSLSSTERGIRSEMGQLRQENELLQNKLHNAVQMKQKDKQNISQLEKKLKAEQEARSFVEKQLMEEKKRKKLEEATAARAVAFAAASRGECTETLRNRIRELEAEGKKLTMDMKVKEDQIRELELKVQELRKYKENEKDTEVLMSALSAMQDKTQHLENSLSAETRIKLDLFSALGDAKRQLEIAQGQILQKDQEIKDLKQKIAEVMAVMPSITYSAATSPLSPVSPHYSSKFVETSPSGLDPNASVYQPLKK

Target ORF information:

RefSeq Version	NM_001282564.1
Organism	Homo sapiens(human)
Definition	Homo sapiens macoilin 1 (MACO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282564.1

ORF Insert Sequence:

ATGAAGCGGC GGAACGCCGA CTGCAGTAAG CTCCGCCGCC CCCTAAAGCG GAACCGGATC 
ACCGAGGGCA TTTACGGCAG TACATTTTTA TACCTGAAAT TCCTGGTGGT GTGGGCACTT 
GTCCTCCTAG CAGATTTTGT CCTGGAGTTC AGATTTGAAT ACCTGTGGCC ATTCTGGCTT 
TTCATCAGAA GCGTCTATGA TTCCTTCAGA TACCAGGGAC TGGCCTTCTC AGTATTTTTT 
GTTTGTGTAG CATTCACGTC AAATATAATA TGCCTGCTGT TCATCCCCAT ACAGTGGCTT 
TTTTTTGCTG CTAGCACATA TGTATGGGTT CAGTACGTAT GGCACACAGA AAGGGGAGTG 
TGTTTGCCTA CAGTGTCTCT CTGGATCCTC TTTGTTTATA TTGAAGCAGC CATTAGATTT 
AAAGATCTCA AAAACTTTCA TGTAGACCTT TGTCGTCCAT TTGCTGCTCA CTGGCTGGAA 
CAAGACATTA AAAAGTTAAA GGCTGACCTG CAAGCCAGCA GACAAGTGGA ACAAGAGCTC 
CGCAGTCAGA TCAGCTCCCT TTCGAGCACC GAGCGAGGGA TCCGCTCAGA AATGGGCCAG 
CTTCGGCAGG AGAACGAGCT GCTGCAGAAC AAGTTACATA ATGCTGTGCA AATGAAGCAA 
AAAGACAAGC AGAATATCAG CCAGTTGGAG AAAAAGCTAA AAGCTGAGCA GGAAGCCCGA 
AGTTTTGTAG AGAAACAGTT AATGGAAGAG AAAAAGAGGA AGAAGTTAGA AGAAGCCACT 
GCTGCCCGGG CTGTTGCGTT TGCTGCTGCA TCTAGGGGAG AATGCACCGA AACCTTACGG 
AATCGGATCA GAGAACTAGA AGCAGAGGGC AAGAAGCTCA CGATGGACAT GAAGGTGAAA 
GAAGACCAAA TCAGAGAACT AGAACTAAAA GTCCAGGAGC TTCGGAAATA TAAGGAAAAT 
GAGAAGGACA CTGAGGTGTT AATGTCAGCC CTCTCAGCCA TGCAAGACAA AACACAGCAC 
CTGGAGAACA GCTTAAGTGC AGAGACGAGA ATCAAGCTGG ACCTGTTCTC CGCACTGGGC 
GATGCAAAGC GGCAGCTCGA GATTGCCCAA GGACAAATCC TTCAGAAAGA TCAGGAAATC 
AAGGACCTAA AACAGAAGAT AGCCGAAGTC ATGGCCGTCA TGCCCAGCAT AACATACAGT 
GCCGCCACCA GCCCCCTGAG CCCTGTTTCC CCCCACTACT CTTCCAAATT TGTGGAGACC 
AGCCCCTCTG GACTTGACCC CAATGCCTCT GTTTACCAGC CCCTGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM57 ( XM_005245931.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245988.1 Homo sapiens macoilin 1 (MACO1), transcript variant X1, mRNA.

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CloneID	OHu33576
Clone ID Related Accession (Same CDS sequence)	XM_005245931.2
Accession Version	XM_005245931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	macoilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGGC GGAACGCCGA CTGCAGTAAG CTCCGCCGCC CCCTAAAGCG GAACCGGATC 
ACCGAGGGCA TTTACGGCAG TACATTTTTA TACCTGAAAT TCCTGGTGGT GTGGGCACTT 
GTCCTCCTAG CAGATTTTGT CCTGGAGTTC AGATTTGAAT ACCTGTGGCC ATTCTGGCTT 
TTCATCAGAA GCGTCTATGA TTCCTTCAGA TACCAGGGAC TGGCCTTCTC AGTATTTTTT 
GTTTGTGTAG CATTCACGTC AAATATAATA TGCCTGCTGT TCATCCCCAT ACAGTGGCTT 
TTTTTTGCTG CTAGCACATA TGTATGGGTT CAGTACGTAT GGCACACAGA AAGGGGAGTG 
TGTTTGCCTA CAGTGTCTCT CTGGATCCTC TTTGTTTATA TTGAAGCAGC CATTAGATTT 
AAAGATCTCA AAAACTTTCA TGTAGACCTT TGTCGTCCAT TTGCTGCTCA CTGTATTGGG 
TACCCTGTGG TAACTTTGGG GTTTGGCTTC AAAAGTTACG TAAGCTACAA AATGCGGTTA 
AGGAAGCAAA AAGAAGTACA AAAAGAGAAC GAGTTTTACA TGCAACTTCT TCAACAAGCT 
CTCCCTCCAG AGCAACAGAT GCTACAGAAG CAAGAAAAAG AGGCCGAGGA AGCAGCCAAA 
GGATTACCTG ATATGGATTC TTCGATCCTT ATACACCACA ATGGAGGTAT CCCAGCCAAC 
AAAAAACTCT CCACAACTTT GCCAGAGATA GAATACCGAG AAAAAGGGAA AGAAAAGGAC 
AAGGATGCCA AAAAACACAA CCTTGGAATA AATAACAACA ATATTCTACA ACCTGTAGAC 
TCTAAAATAC AAGAGATTGA GTATATGGAA AACCATATCA ATAGTAAAAG ATTAAATAAT 
GATCTTGTGG GAAGTACAGA AAATCTCTTG AAAGAGGACT CATGCACTGC TTCCTCAAAA 
AATTACAAAA ATGCCAGTGG AGTTGTGAAC TCTTCACCTC GAAGTCATAG CGCCACAAAT 
GGGAGCATTC CTTCCTCATC TAGTAAAAAT GAGAAGAAGC AGAAATGCAC TAGCAAGAGC 
CCAAGTACAC ACAAGGACTT AATGGAAAAC TGTATTCCTA ATAACCAGCT AAGCAAACCA 
GACGCACTGG TCAGGCTGGA ACAAGACATT AAAAAGTTAA AGGCTGACCT GCAAGCCAGC 
AGACAAGTGG AACAAGAGCT CCGCAGTCAG ATCAGCTCCC TTTCGAGCAC CGAGCGAGGG 
ATCCGCTCAG AAATGGGCCA GCTTCGGCAG GAGAACGAGC TGCTGCAGAA CAAGTTACAT 
AATGCTGTGC AAATGAAGCA AAAAGACAAG CAGAATATCA GCCAGTTGGA GAAAAAGCTA 
AAAGCTGAGC AGGAAGCCCG AAGTTTTGTA GAGAAACAGT TAATGGAAGA GAAAAAGAGG 
AAGAAGTTAG AAGAAGCCAC TGCTGCCCGG GCTGTTGCGT TTGCTGCTGC ATCTAGGGGA 
GAATGCACCG AAACCTTACG GAATCGGATC AGAGAACTAG AAGCAGAGGG CAAGAAGCTC 
ACGATGGACA TGAAGGTGAA AGAAGACCAA ATCAGAGAAC TAGAACTAAA AGTCCAGGCT 
AATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245988.1
CDS	203..1831
Translation	MKRRNADCSKLRRPLKRNRITEGIYGSTFLYLKFLVVWALVLLADFVLEFRFEYLWPFWLFIRSVYDSFRYQGLAFSVFFVCVAFTSNIICLLFIPIQWLFFAASTYVWVQYVWHTERGVCLPTVSLWILFVYIEAAIRFKDLKNFHVDLCRPFAAHCIGYPVVTLGFGFKSYVSYKMRLRKQKEVQKENEFYMQLLQQALPPEQQMLQKQEKEAEEAAKGLPDMDSSILIHHNGGIPANKKLSTTLPEIEYREKGKEKDKDAKKHNLGINNNNILQPVDSKIQEIEYMENHINSKRLNNDLVGSTENLLKEDSCTASSKNYKNASGVVNSSPRSHSATNGSIPSSSSKNEKKQKCTSKSPSTHKDLMENCIPNNQLSKPDALVRLEQDIKKLKADLQASRQVEQELRSQISSLSSTERGIRSEMGQLRQENELLQNKLHNAVQMKQKDKQNISQLEKKLKAEQEARSFVEKQLMEEKKRKKLEEATAARAVAFAAASRGECTETLRNRIRELEAEGKKLTMDMKVKEDQIRELELKVQANL

Target ORF information:

RefSeq Version	XM_005245931.2
Organism	Homo sapiens(human)
Definition	Homo sapiens macoilin 1 (MACO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245931.2

ORF Insert Sequence:

ATGAAGCGGC GGAACGCCGA CTGCAGTAAG CTCCGCCGCC CCCTAAAGCG GAACCGGATC 
ACCGAGGGCA TTTACGGCAG TACATTTTTA TACCTGAAAT TCCTGGTGGT GTGGGCACTT 
GTCCTCCTAG CAGATTTTGT CCTGGAGTTC AGATTTGAAT ACCTGTGGCC ATTCTGGCTT 
TTCATCAGAA GCGTCTATGA TTCCTTCAGA TACCAGGGAC TGGCCTTCTC AGTATTTTTT 
GTTTGTGTAG CATTCACGTC AAATATAATA TGCCTGCTGT TCATCCCCAT ACAGTGGCTT 
TTTTTTGCTG CTAGCACATA TGTATGGGTT CAGTACGTAT GGCACACAGA AAGGGGAGTG 
TGTTTGCCTA CAGTGTCTCT CTGGATCCTC TTTGTTTATA TTGAAGCAGC CATTAGATTT 
AAAGATCTCA AAAACTTTCA TGTAGACCTT TGTCGTCCAT TTGCTGCTCA CTGTATTGGG 
TACCCTGTGG TAACTTTGGG GTTTGGCTTC AAAAGTTACG TAAGCTACAA AATGCGGTTA 
AGGAAGCAAA AAGAAGTACA AAAAGAGAAC GAGTTTTACA TGCAACTTCT TCAACAAGCT 
CTCCCTCCAG AGCAACAGAT GCTACAGAAG CAAGAAAAAG AGGCCGAGGA AGCAGCCAAA 
GGATTACCTG ATATGGATTC TTCGATCCTT ATACACCACA ATGGAGGTAT CCCAGCCAAC 
AAAAAACTCT CCACAACTTT GCCAGAGATA GAATACCGAG AAAAAGGGAA AGAAAAGGAC 
AAGGATGCCA AAAAACACAA CCTTGGAATA AATAACAACA ATATTCTACA ACCTGTAGAC 
TCTAAAATAC AAGAGATTGA GTATATGGAA AACCATATCA ATAGTAAAAG ATTAAATAAT 
GATCTTGTGG GAAGTACAGA AAATCTCTTG AAAGAGGACT CATGCACTGC TTCCTCAAAA 
AATTACAAAA ATGCCAGTGG AGTTGTGAAC TCTTCACCTC GAAGTCATAG CGCCACAAAT 
GGGAGCATTC CTTCCTCATC TAGTAAAAAT GAGAAGAAGC AGAAATGCAC TAGCAAGAGC 
CCAAGTACAC ACAAGGACTT AATGGAAAAC TGTATTCCTA ATAACCAGCT AAGCAAACCA 
GACGCACTGG TCAGGCTGGA ACAAGACATT AAAAAGTTAA AGGCTGACCT GCAAGCCAGC 
AGACAAGTGG AACAAGAGCT CCGCAGTCAG ATCAGCTCCC TTTCGAGCAC CGAGCGAGGG 
ATCCGCTCAG AAATGGGCCA GCTTCGGCAG GAGAACGAGC TGCTGCAGAA CAAGTTACAT 
AATGCTGTGC AAATGAAGCA AAAAGACAAG CAGAATATCA GCCAGTTGGA GAAAAAGCTA 
AAAGCTGAGC AGGAAGCCCG AAGTTTTGTA GAGAAACAGT TAATGGAAGA GAAAAAGAGG 
AAGAAGTTAG AAGAAGCCAC TGCTGCCCGG GCTGTTGCGT TTGCTGCTGC ATCTAGGGGA 
GAATGCACCG AAACCTTACG GAATCGGATC AGAGAACTAG AAGCAGAGGG CAAGAAGCTC 
ACGATGGACA TGAAGGTGAA AGAAGACCAA ATCAGAGAAC TAGAACTAAA AGTCCAGGCT 
AATTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polymorphism of rs873308 near the transmembrane protein 57 gene is associated with serum lipid levels.
Bioscience reports34(2)(2014 Apr)
Guo T,Yin RX,Lin QZ,Wu J,Shen SW,Sun JQ,Shi GY,Wu JZ,Li H,Wang YM

Discovery and refinement of loci associated with lipid levels.
Nature genetics45(11)1274-1283(2013 Nov)
Willer CJ,Schmidt EM,Sengupta S,Peloso GM,Gustafsson S,Kanoni S,Ganna A,Chen J,Buchkovich ML,Mora S,Beckmann JS,Bragg-Gresham JL,Chang HY,Demirkan A,Den Hertog HM,Do R,Donnelly LA,Ehret GB,Esko T,Feitosa MF,Ferreira T,Fischer K,Fontanillas P,Fraser RM,Freitag DF,Gurdasani D,Heikkil? K,Hypp?nen E,Isaacs A,Jackson AU,Johansson ?,Johnson T,Kaakinen M,Kettunen J,Kleber ME,Li X,Luan J,Lyytik?inen LP,Magnusson PKE,Mangino M,Mihailov E,Montasser ME,M?ller-Nurasyid M,Nolte IM,O'Connell JR,Palmer CD,Perola M,Petersen AK,Sanna S,Saxena R,Service SK,Shah S,Shungin D,Sidore C,Song C,Strawbridge RJ,Surakka I,Tanaka T,Teslovich TM,Thorleifsson G,Van den Herik EG,Voight BF,Volcik KA,Waite LL,Wong A,Wu Y,Zhang W,Absher D,Asiki G,Barroso I,Been LF,Bolton JL,Bonnycastle LL,Brambilla P,Burnett MS,Cesana G,Dimitriou M,Doney ASF,D?ring A,Elliott P,Epstein SE,Ingi Eyjolfsson G,Gigante B,Goodarzi MO,Grallert H,Gravito ML,Groves CJ,Hallmans G,Hartikainen AL,Hayward C,Hernandez D,Hicks AA,Holm H,Hung YJ,Illig T,Jones MR,Kaleebu P,Kastelein JJP,Khaw KT,Kim E,Klopp N,Komulainen P,Kumari M,Langenberg C,Lehtim?ki T,Lin SY,Lindstr?m J,Loos RJF,Mach F,McArdle WL,Meisinger C,Mitchell BD,M?ller G,Nagaraja R,Narisu N,Nieminen TVM,Nsubuga RN,Olafsson I,Ong KK,Palotie A,Papamarkou T,Pomilla C,Pouta A,Rader DJ,Reilly MP,Ridker PM,Rivadeneira F,Rudan I,Ruokonen A,Samani N,Scharnagl H,Seeley J,Silander K,Stan??kov? A,Stirrups K,Swift AJ,Tiret L,Uitterlinden AG,van Pelt LJ,Vedantam S,Wainwright N,Wijmenga C,Wild SH,Willemsen G,Wilsgaard T,Wilson JF,Young EH,Zhao JH,Adair LS,Arveiler D,Assimes TL,Bandinelli S,Bennett F,Bochud M,Boehm BO,Boomsma DI,Borecki IB,Bornstein SR,Bovet P,Burnier M,Campbell H,Chakravarti A,Chambers JC,Chen YI,Collins FS,Cooper RS,Danesh J,Dedoussis G,de Faire U,Feranil AB,Ferri?res J,Ferrucci L,Freimer NB,Gieger C,Groop LC,Gudnason V,Gyllensten U,Hamsten A,Harris TB,Hingorani A,Hirschhorn JN,Hofman A,Hovingh GK,Hsiung CA,Humphries SE,Hunt SC,Hveem K,Iribarren C,J?rvelin MR,Jula A,K?h?nen M,Kaprio J,Kes?niemi A,Kivimaki M,Kooner JS,Koudstaal PJ,Krauss RM,Kuh D,Kuusisto J,Kyvik KO,Laakso M,Lakka TA,Lind L,Lindgren CM,Martin NG,M?rz W,McCarthy MI,McKenzie CA,Meneton P,Metspalu A,Moilanen L,Morris AD,Munroe PB,Nj?lstad I,Pedersen NL,Power C,Pramstaller PP,Price JF,Psaty BM,Quertermous T,Rauramaa R,Saleheen D,Salomaa V,Sanghera DK,Saramies J,Schwarz PEH,Sheu WH,Shuldiner AR,Siegbahn A,Spector TD,Stefansson K,Strachan DP,Tayo BO,Tremoli E,Tuomilehto J,Uusitupa M,van Duijn CM,Vollenweider P,Wallentin L,Wareham NJ,Whitfield JB,Wolffenbuttel BHR,Ordovas JM,Boerwinkle E,Palmer CNA,Thorsteinsdottir U,Chasman DI,Rotter JI,Franks PW,Ripatti S,Cupples LA,Sandhu MS,Rich SS,Boehnke M,Deloukas P,Kathiresan S,Mohlke KL,Ingelsson E,Abecasis GR,

Complement receptor 1 gene variants are associated with erythrocyte sedimentation rate.
American journal of human genetics89(1)131-8(2011 Jul)
Kullo IJ,Ding K,Shameer K,McCarty CA,Jarvik GP,Denny JC,Ritchie MD,Ye Z,Crosslin DR,Chisholm RL,Manolio TA,Chute CG

Novel and conserved protein macoilin is required for diverse neuronal functions in Caenorhabditis elegans.
PLoS genetics7(5)e1001384(2011 May)
Miyara A,Ohta A,Okochi Y,Tsukada Y,Kuhara A,Mori I

Macoilin, a conserved nervous system-specific ER membrane protein that regulates neuronal excitability.
PLoS genetics7(3)e1001341(2011 Mar)
Arellano-Carbajal F,Brise?o-Roa L,Couto A,Cheung BH,Labouesse M,de Bono M

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TMEM57 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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