AUP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AUP1
Entrez Gene ID	550
Full Name	AUP1, lipid droplet regulating VLDL assembly factor
General protein information	Preferred Names AUP1, lipid droplet regulating VLDL assembly factor Names ancient ubiquitous protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.1
Summary	The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014].
Disorder MIM:	602434

mRNA and Protein(s)

mRNA	Protein	Name
XM_005264392.3	XP_005264449.1	ancient ubiquitous protein 1 isoform X1
NM_181575.4	NP_853553.1	ancient ubiquitous protein 1
NM_181575.5	NP_853553.1	ancient ubiquitous protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AUP1	XP_852590.2
Caenorhabditis elegans (roundworm)	F44B9.5	NP_498746.1
	LOC710020	XP_002808089.1
Rattus norvegicus (Norway rat)	Aup1	NP_001073368.1
Homo sapiens (human)	AUP1	NP_853553.1
Pan troglodytes (chimpanzee)	AUP1	XP_001161094.1
Bos taurus (cattle)	AUP1	NP_001015555.1
Mus musculus (house mouse)	Aup1	NP_031543.2
Gallus gallus (chicken)	AUP1	XP_001232437.3
Danio rerio (zebrafish)	aup1	NP_955984.2
Xenopus tropicalis (tropical clawed frog)	aup1	XP_004921063.1

Gene Ontology
Bibliography

Related articles in PubMed

AUP1 (Ancient Ubiquitous Protein 1) Is a Key Determinant of Hepatic Very-Low-Density Lipoprotein Assembly and Secretion.
Zhang J, Zamani M, Thiele C, Taher J, Amir Alipour M, Yao Z, Adeli K
Arteriosclerosis, thrombosis, and vascular biology37(4)633-642(2017 Apr)

Identification of ERAD components essential for dislocation of the null Hong Kong variant of ?-1-antitrypsin (NHK).
Zhong Y, Shen H, Wang Y, Yang Y, Yang P, Fang S
Biochemical and biophysical research communications458(2)424-8(2015 Mar)

Monotopic topology is required for lipid droplet targeting of ancient ubiquitous protein 1.
Stevanovic A, Thiele C
Journal of lipid research54(2)503-13(2013 Feb)

MOR is not enough: identification of novel mu-opioid receptor interacting proteins using traditional and modified membrane yeast two-hybrid screens.
Petko J, Justice-Bitner S, Jin J, Wong V, Kittanakom S, Ferraro TN, Stagljar I, Levenson R
PloS one8(6)e67608(2013)

Monoubiquitination of ancient ubiquitous protein 1 promotes lipid droplet clustering.
Lohmann D, Spandl J, Stevanovic A, Schoene M, Philippou-Massier J, Thiele C
PloS one8(9)e72453(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AUP1 may be a crucial factor in hepatic apoB100 quality control, determining the rate at which apoB100 is degraded or lipidated to enable VLDL particle assembly and secretion.
Title: AUP1 (Ancient Ubiquitous Protein 1) Is a Key Determinant of Hepatic Very-Low-Density Lipoprotein Assembly and Secretion.

A novel role for Aup1 in maintenance of intracellular cholesterol homeostasis via mediation of the endoplasmic reticulum associated degradation of HMG-CoA reductase.
Title: Ancient ubiquitous protein-1 mediates sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl CoA reductase in lipid droplet-associated endoplasmic reticulum membranes.

AUP1 targeting signals
Title: Monotopic topology is required for lipid droplet targeting of ancient ubiquitous protein 1.

Monoubiquitinated AUP1 on the lipid droplet surface specifically induces clustering.
Title: Monoubiquitination of ancient ubiquitous protein 1 promotes lipid droplet clustering.

Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet accumulation and endoplasmic reticulum (ER) protein quality control.
Title: Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet accumulation and endoplasmic reticulum (ER) protein quality control.

The following AUP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AUP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21941	NM_181575.5 Latest version!	Homo sapiens AUP1 lipid droplet regulating VLDL assembly factor (AUP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$244.30 $349.00
OHu35456	XM_005264392.3 Latest version!	Homo sapiens ancient ubiquitous protein 1 (AUP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu21941	NM_181575.4	Homo sapiens AUP1, lipid droplet regulating VLDL assembly factor (AUP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$244.30 $349.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AUP1 ( NM_181575.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_853553.1 Homo sapiens AUP1, lipid droplet regulating VLDL assembly factor (AUP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21941
Clone ID Related Accession (Same CDS sequence)	NM_181575.4 , NM_181575.5
Accession Version	NM_181575.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Homo sapiens(human)
Product	ancient ubiquitous protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY078082.1, AF165515.1 and AI580873.1. This sequence is a reference standard in the RefSeqGene project. On Nov 25, 2014 this sequence version replaced NM_181575.3. Transcript Variant: This variant (2) represents the shortest transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.584614.1, SRR1163655.500782.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTTC CCTCAGGGCC GGGGCCGGAG CGGCTCTTTG ACTCGCACCG GCTTCCGGGT 
GACTGCTTCC TACTGCTCGT GCTGCTGCTC TACGCGCCAG TCGGGTTCTG CCTCCTCGTC 
CTGCGCCTCT TTCTCGGGAT CCACGTCTTC CTGGTCAGCT GCGCGCTGCC AGACAGCGTC 
CTTCGCAGAT TCGTAGTGCG GACCATGTGT GCGGTGCTAG GGCTCGTGGC CCGGCAGGAG 
GACTCCGGAC TCCGGGATCA CAGTGTCAGG GTCCTCATTT CCAACCATGT GACACCTTTC 
GACCACAACA TAGTCAATTT GCTTACCACC TGTAGCACCC CTCTACTCAA TAGTCCCCCC 
AGCTTTGTGT GCTGGTCTCG GGGCTTCATG GAGATGAATG GGCGGGGGGA GTTGGTGGAG 
TCACTCAAGA GATTCTGTGC TTCCACGAGG CTTCCCCCCA CTCCTCTGCT GCTATTCCCT 
GAGGAAGAGG CCACCAATGG CCGGGAGGGG CTCCTGCGCT TCAGTTCCTG GCCATTTTCT 
ATCCAAGATG TGGTACAACC TCTTACCCTG CAAGTTCAGA GACCCCTGGT CTCTGTGACG 
GTGTCAGATG CCTCCTGGGT CTCAGAACTG CTGTGGTCAC TTTTCGTCCC TTTCACGGTG 
TATCAAGTAA GGTGGCTTCG TCCTGTTCAT CGCCAACTAG GGGAAGCGAA TGAGGAGTTT 
GCACTCCGTG TACAACAGCT GGTGGCCAAG GAATTGGGCC AGACAGGGAC ACGGCTCACT 
CCAGCTGACA AAGCAGAGCA CATGAAGCGA CAAAGACACC CCAGATTGCG CCCCCAGTCA 
GCCCAGTCTT CTTTCCCTCC CTCCCCTGGT CCTTCTCCTG ATGTGCAACT GGCAACTCTG 
GCTCAGAGAG TCAAGGAAGT TTTGCCCCAT GTGCCATTGG GTGTCATCCA GAGAGACCTG 
GCCAAGACTG GCTGTGTAGA CTTGACTATC ACTAATCTGC TTGAGGGGGC CGTAGCTTTC 
ATGCCTGAAG ACATCACCAA GGGAACTCAG TCCCTACCCA CAGCCTCTGC CTCCAAGTTT 
CCCAGCTCTG GCCCGGTGAC CCCTCAGCCA ACAGCCCTAA CATTTGCCAA GTCTTCCTGG 
GCCCGGCAGG AGAGCCTGCA GGAGCGCAAG CAAGCACTAT ATGAATACGC AAGAAGGAGA 
TTCACAGAGA GACGAGCCCA GGAGGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853553.1
CDS	269..1501
Translation	MELPSGPGPERLFDSHRLPGDCFLLLVLLLYAPVGFCLLVLRLFLGIHVFLVSCALPDSVLRRFVVRTMCAVLGLVARQEDSGLRDHSVRVLISNHVTPFDHNIVNLLTTCSTPLLNSPPSFVCWSRGFMEMNGRGELVESLKRFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSIQDVVQPLTLQVQRPLVSVTVSDASWVSELLWSLFVPFTVYQVRWLRPVHRQLGEANEEFALRVQQLVAKELGQTGTRLTPADKAEHMKRQRHPRLRPQSAQSSFPPSPGPSPDVQLATLAQRVKEVLPHVPLGVIQRDLAKTGCVDLTITNLLEGAVAFMPEDITKGTQSLPTASASKFPSSGPVTPQPTALTFAKSSWARQESLQERKQALYEYARRRFTERRAQEAD

Target ORF information:

RefSeq Version	NM_181575.4
Organism	Homo sapiens(human)
Definition	Homo sapiens AUP1, lipid droplet regulating VLDL assembly factor (AUP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181575.4

ORF Insert Sequence:

ATGGAGCTTC CCTCAGGGCC GGGGCCGGAG CGGCTCTTTG ACTCGCACCG GCTTCCGGGT 
GACTGCTTCC TACTGCTCGT GCTGCTGCTC TACGCGCCAG TCGGGTTCTG CCTCCTCGTC 
CTGCGCCTCT TTCTCGGGAT CCACGTCTTC CTGGTCAGCT GCGCGCTGCC AGACAGCGTC 
CTTCGCAGAT TCGTAGTGCG GACCATGTGT GCGGTGCTAG GGCTCGTGGC CCGGCAGGAG 
GACTCCGGAC TCCGGGATCA CAGTGTCAGG GTCCTCATTT CCAACCATGT GACACCTTTC 
GACCACAACA TAGTCAATTT GCTTACCACC TGTAGCACCC CTCTACTCAA TAGTCCCCCC 
AGCTTTGTGT GCTGGTCTCG GGGCTTCATG GAGATGAATG GGCGGGGGGA GTTGGTGGAG 
TCACTCAAGA GATTCTGTGC TTCCACGAGG CTTCCCCCCA CTCCTCTGCT GCTATTCCCT 
GAGGAAGAGG CCACCAATGG CCGGGAGGGG CTCCTGCGCT TCAGTTCCTG GCCATTTTCT 
ATCCAAGATG TGGTACAACC TCTTACCCTG CAAGTTCAGA GACCCCTGGT CTCTGTGACG 
GTGTCAGATG CCTCCTGGGT CTCAGAACTG CTGTGGTCAC TTTTCGTCCC TTTCACGGTG 
TATCAAGTAA GGTGGCTTCG TCCTGTTCAT CGCCAACTAG GGGAAGCGAA TGAGGAGTTT 
GCACTCCGTG TACAACAGCT GGTGGCCAAG GAATTGGGCC AGACAGGGAC ACGGCTCACT 
CCAGCTGACA AAGCAGAGCA CATGAAGCGA CAAAGACACC CCAGATTGCG CCCCCAGTCA 
GCCCAGTCTT CTTTCCCTCC CTCCCCTGGT CCTTCTCCTG ATGTGCAACT GGCAACTCTG 
GCTCAGAGAG TCAAGGAAGT TTTGCCCCAT GTGCCATTGG GTGTCATCCA GAGAGACCTG 
GCCAAGACTG GCTGTGTAGA CTTGACTATC ACTAATCTGC TTGAGGGGGC CGTAGCTTTC 
ATGCCTGAAG ACATCACCAA GGGAACTCAG TCCCTACCCA CAGCCTCTGC CTCCAAGTTT 
CCCAGCTCTG GCCCGGTGAC CCCTCAGCCA ACAGCCCTAA CATTTGCCAA GTCTTCCTGG 
GCCCGGCAGG AGAGCCTGCA GGAGCGCAAG CAAGCACTAT ATGAATACGC AAGAAGGAGA 
TTCACAGAGA GACGAGCCCA GGAGGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AUP1 ( NM_181575.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_853553.1 Homo sapiens AUP1 lipid droplet regulating VLDL assembly factor (AUP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21941
Clone ID Related Accession (Same CDS sequence)	NM_181575.4 , NM_181575.5
Accession Version	NM_181575.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	ancient ubiquitous protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY078082.1, AF165515.1 and AI580873.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_181575.4. Transcript Variant: This variant (2) represents the shortest transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.559767.1, SRR1163655.500782.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000377526.4/ ENSP00000366748.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCTTC CCTCAGGGCC GGGGCCGGAG CGGCTCTTTG ACTCGCACCG GCTTCCGGGT 
GACTGCTTCC TACTGCTCGT GCTGCTGCTC TACGCGCCAG TCGGGTTCTG CCTCCTCGTC 
CTGCGCCTCT TTCTCGGGAT CCACGTCTTC CTGGTCAGCT GCGCGCTGCC AGACAGCGTC 
CTTCGCAGAT TCGTAGTGCG GACCATGTGT GCGGTGCTAG GGCTCGTGGC CCGGCAGGAG 
GACTCCGGAC TCCGGGATCA CAGTGTCAGG GTCCTCATTT CCAACCATGT GACACCTTTC 
GACCACAACA TAGTCAATTT GCTTACCACC TGTAGCACCC CTCTACTCAA TAGTCCCCCC 
AGCTTTGTGT GCTGGTCTCG GGGCTTCATG GAGATGAATG GGCGGGGGGA GTTGGTGGAG 
TCACTCAAGA GATTCTGTGC TTCCACGAGG CTTCCCCCCA CTCCTCTGCT GCTATTCCCT 
GAGGAAGAGG CCACCAATGG CCGGGAGGGG CTCCTGCGCT TCAGTTCCTG GCCATTTTCT 
ATCCAAGATG TGGTACAACC TCTTACCCTG CAAGTTCAGA GACCCCTGGT CTCTGTGACG 
GTGTCAGATG CCTCCTGGGT CTCAGAACTG CTGTGGTCAC TTTTCGTCCC TTTCACGGTG 
TATCAAGTAA GGTGGCTTCG TCCTGTTCAT CGCCAACTAG GGGAAGCGAA TGAGGAGTTT 
GCACTCCGTG TACAACAGCT GGTGGCCAAG GAATTGGGCC AGACAGGGAC ACGGCTCACT 
CCAGCTGACA AAGCAGAGCA CATGAAGCGA CAAAGACACC CCAGATTGCG CCCCCAGTCA 
GCCCAGTCTT CTTTCCCTCC CTCCCCTGGT CCTTCTCCTG ATGTGCAACT GGCAACTCTG 
GCTCAGAGAG TCAAGGAAGT TTTGCCCCAT GTGCCATTGG GTGTCATCCA GAGAGACCTG 
GCCAAGACTG GCTGTGTAGA CTTGACTATC ACTAATCTGC TTGAGGGGGC CGTAGCTTTC 
ATGCCTGAAG ACATCACCAA GGGAACTCAG TCCCTACCCA CAGCCTCTGC CTCCAAGTTT 
CCCAGCTCTG GCCCGGTGAC CCCTCAGCCA ACAGCCCTAA CATTTGCCAA GTCTTCCTGG 
GCCCGGCAGG AGAGCCTGCA GGAGCGCAAG CAAGCACTAT ATGAATACGC AAGAAGGAGA 
TTCACAGAGA GACGAGCCCA GGAGGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853553.1
CDS	78..1310
Translation	MELPSGPGPERLFDSHRLPGDCFLLLVLLLYAPVGFCLLVLRLFLGIHVFLVSCALPDSVLRRFVVRTMCAVLGLVARQEDSGLRDHSVRVLISNHVTPFDHNIVNLLTTCSTPLLNSPPSFVCWSRGFMEMNGRGELVESLKRFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSIQDVVQPLTLQVQRPLVSVTVSDASWVSELLWSLFVPFTVYQVRWLRPVHRQLGEANEEFALRVQQLVAKELGQTGTRLTPADKAEHMKRQRHPRLRPQSAQSSFPPSPGPSPDVQLATLAQRVKEVLPHVPLGVIQRDLAKTGCVDLTITNLLEGAVAFMPEDITKGTQSLPTASASKFPSSGPVTPQPTALTFAKSSWARQESLQERKQALYEYARRRFTERRAQEAD

Target ORF information:

RefSeq Version	NM_181575.5
Organism	Homo sapiens(human)
Definition	Homo sapiens AUP1 lipid droplet regulating VLDL assembly factor (AUP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181575.5

ORF Insert Sequence:

ATGGAGCTTC CCTCAGGGCC GGGGCCGGAG CGGCTCTTTG ACTCGCACCG GCTTCCGGGT 
GACTGCTTCC TACTGCTCGT GCTGCTGCTC TACGCGCCAG TCGGGTTCTG CCTCCTCGTC 
CTGCGCCTCT TTCTCGGGAT CCACGTCTTC CTGGTCAGCT GCGCGCTGCC AGACAGCGTC 
CTTCGCAGAT TCGTAGTGCG GACCATGTGT GCGGTGCTAG GGCTCGTGGC CCGGCAGGAG 
GACTCCGGAC TCCGGGATCA CAGTGTCAGG GTCCTCATTT CCAACCATGT GACACCTTTC 
GACCACAACA TAGTCAATTT GCTTACCACC TGTAGCACCC CTCTACTCAA TAGTCCCCCC 
AGCTTTGTGT GCTGGTCTCG GGGCTTCATG GAGATGAATG GGCGGGGGGA GTTGGTGGAG 
TCACTCAAGA GATTCTGTGC TTCCACGAGG CTTCCCCCCA CTCCTCTGCT GCTATTCCCT 
GAGGAAGAGG CCACCAATGG CCGGGAGGGG CTCCTGCGCT TCAGTTCCTG GCCATTTTCT 
ATCCAAGATG TGGTACAACC TCTTACCCTG CAAGTTCAGA GACCCCTGGT CTCTGTGACG 
GTGTCAGATG CCTCCTGGGT CTCAGAACTG CTGTGGTCAC TTTTCGTCCC TTTCACGGTG 
TATCAAGTAA GGTGGCTTCG TCCTGTTCAT CGCCAACTAG GGGAAGCGAA TGAGGAGTTT 
GCACTCCGTG TACAACAGCT GGTGGCCAAG GAATTGGGCC AGACAGGGAC ACGGCTCACT 
CCAGCTGACA AAGCAGAGCA CATGAAGCGA CAAAGACACC CCAGATTGCG CCCCCAGTCA 
GCCCAGTCTT CTTTCCCTCC CTCCCCTGGT CCTTCTCCTG ATGTGCAACT GGCAACTCTG 
GCTCAGAGAG TCAAGGAAGT TTTGCCCCAT GTGCCATTGG GTGTCATCCA GAGAGACCTG 
GCCAAGACTG GCTGTGTAGA CTTGACTATC ACTAATCTGC TTGAGGGGGC CGTAGCTTTC 
ATGCCTGAAG ACATCACCAA GGGAACTCAG TCCCTACCCA CAGCCTCTGC CTCCAAGTTT 
CCCAGCTCTG GCCCGGTGAC CCCTCAGCCA ACAGCCCTAA CATTTGCCAA GTCTTCCTGG 
GCCCGGCAGG AGAGCCTGCA GGAGCGCAAG CAAGCACTAT ATGAATACGC AAGAAGGAGA 
TTCACAGAGA GACGAGCCCA GGAGGCTGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AUP1 ( XM_005264392.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264449.1 Homo sapiens ancient ubiquitous protein 1 (AUP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu35456
Clone ID Related Accession (Same CDS sequence)	XM_005264392.3
Accession Version	XM_005264392.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ancient ubiquitous protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264392.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAAG ACTCCGCCTT CCCAAGAGCC CCTGCGGCCG GGCGCGAAAA TGGCGGCGGC 
GGCGACGGCC GGGCGCTCCT GAAGCAGCAG TTATGGAGCT TCCCTCAGGG CCGGGGCCGG 
AGCGGCTCTT TGACTCGCAC CGGCTTCCGG GTGACTGCTT CCTACTGCTC GTGCTGCTGC 
TCTACGCGCC AGTCGGGTTC TGCCTCCTCG TCCTGCGCCT CTTTCTCGGG ATCCACGTCT 
TCCTGGTCAG CTGCGCGCTG CCAGACAGCG TCCTTCGCAG GTTCCGACGC GGGCGTTCGG 
GGAGTGTCAG AGCTGGGTCT GGCCCGAGGC CACACAGTCA CCACCTCCTG TGTCCCCAGA 
TTCGTAGTGC GGACCATGTG TGCGGTGCTA GGGCTCGTGG CCCGGCAGGA GGACTCCGGA 
CTCCGGGATC ACAGTGTCAG GGTCCTCATT TCCAACCATG TGACACCTTT CGACCACAAC 
ATAGTCAATT TGCTTACCAC CTGTAGCACC CCTCTACTCA ATAGTCCCCC CAGCTTTGTG 
TGCTGGTCTC GGGGCTTCAT GGAGATGAAT GGGCGGGGGG AGTTGGTGGA GTCACTCAAG 
AGATTCTGTG CTTCCACGAG GCTTCCCCCC ACTCCTCTGC TGCTATTCCC TGAGGAAGAG 
GCCACCAATG GCCGGGAGGG GCTCCTGCGC TTCAGTTCCT GGCCATTTTC TATCCAAGAT 
GTGGTACAAC CTCTTACCCT GCAAGTTCAG AGACCCCTGG TCTCTGTGAC GGTGTCAGAT 
GCCTCCTGGG TCTCAGAACT GCTGTGGTCA CTTTTCGTCC CTTTCACGGT GTATCAAGTA 
AGGTGGCTTC GTCCTGTTCA TCGCCAACTA GGGGAAGCGA ATGAGGAGTT TGCACTCCGT 
GTACAACAGC TGGTGGCCAA GGAATTGGGC CAGACAGGGA CACGGCTCAC TCCAGCTGAC 
AAAGCAGAGC ACATGAAGCG ACAAAGACAC CCCAGATTGC GCCCCCAGTC AGCCCAGTCT 
TCTTTCCCTC CCTCCCCTGG TCCTTCTCCT GATGTGCAAC TGGCAACTCT GGCTCAGAGA 
GTCAAGGAAG TTTTGCCCCA TGTGCCATTG GGTGTCATCC AGAGAGACCT GGCCAAGACT 
GGCTGTGTAG ACTTGACTAT CACTAATCTG CTTGAGGGGG CCGTAGCTTT CATGCCTGAA 
GACATCACCA AGGGAACTCA GTCCCTACCC ACAGCCTCTG CCTCCAAGTT TCCCAGCTCT 
GGCCCGGTGA CCCCTCAGCC AACAGCCCTA ACATTTGCCA AGTCTTCCTG GGCCCGGCAG 
GAGAGCCTGC AGGAGCGCAA GCAAGCACTA TATGAATACG CAAGAAGGAG ATTCACAGAG 
AGACGAGCCC AGGAGGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264449.1
CDS	43..1446
Translation	MPKDSAFPRAPAAGRENGGGGDGRALLKQQLWSFPQGRGRSGSLTRTGFRVTASYCSCCCSTRQSGSASSSCASFSGSTSSWSAARCQTASFAGSDAGVRGVSELGLARGHTVTTSCVPRFVVRTMCAVLGLVARQEDSGLRDHSVRVLISNHVTPFDHNIVNLLTTCSTPLLNSPPSFVCWSRGFMEMNGRGELVESLKRFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSIQDVVQPLTLQVQRPLVSVTVSDASWVSELLWSLFVPFTVYQVRWLRPVHRQLGEANEEFALRVQQLVAKELGQTGTRLTPADKAEHMKRQRHPRLRPQSAQSSFPPSPGPSPDVQLATLAQRVKEVLPHVPLGVIQRDLAKTGCVDLTITNLLEGAVAFMPEDITKGTQSLPTASASKFPSSGPVTPQPTALTFAKSSWARQESLQERKQALYEYARRRFTERRAQEAD

Target ORF information:

RefSeq Version	XM_005264392.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ancient ubiquitous protein 1 (AUP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264392.3

ORF Insert Sequence:

ATGCCCAAAG ACTCCGCCTT CCCAAGAGCC CCTGCGGCCG GGCGCGAAAA TGGCGGCGGC 
GGCGACGGCC GGGCGCTCCT GAAGCAGCAG TTATGGAGCT TCCCTCAGGG CCGGGGCCGG 
AGCGGCTCTT TGACTCGCAC CGGCTTCCGG GTGACTGCTT CCTACTGCTC GTGCTGCTGC 
TCTACGCGCC AGTCGGGTTC TGCCTCCTCG TCCTGCGCCT CTTTCTCGGG ATCCACGTCT 
TCCTGGTCAG CTGCGCGCTG CCAGACAGCG TCCTTCGCAG GTTCCGACGC GGGCGTTCGG 
GGAGTGTCAG AGCTGGGTCT GGCCCGAGGC CACACAGTCA CCACCTCCTG TGTCCCCAGA 
TTCGTAGTGC GGACCATGTG TGCGGTGCTA GGGCTCGTGG CCCGGCAGGA GGACTCCGGA 
CTCCGGGATC ACAGTGTCAG GGTCCTCATT TCCAACCATG TGACACCTTT CGACCACAAC 
ATAGTCAATT TGCTTACCAC CTGTAGCACC CCTCTACTCA ATAGTCCCCC CAGCTTTGTG 
TGCTGGTCTC GGGGCTTCAT GGAGATGAAT GGGCGGGGGG AGTTGGTGGA GTCACTCAAG 
AGATTCTGTG CTTCCACGAG GCTTCCCCCC ACTCCTCTGC TGCTATTCCC TGAGGAAGAG 
GCCACCAATG GCCGGGAGGG GCTCCTGCGC TTCAGTTCCT GGCCATTTTC TATCCAAGAT 
GTGGTACAAC CTCTTACCCT GCAAGTTCAG AGACCCCTGG TCTCTGTGAC GGTGTCAGAT 
GCCTCCTGGG TCTCAGAACT GCTGTGGTCA CTTTTCGTCC CTTTCACGGT GTATCAAGTA 
AGGTGGCTTC GTCCTGTTCA TCGCCAACTA GGGGAAGCGA ATGAGGAGTT TGCACTCCGT 
GTACAACAGC TGGTGGCCAA GGAATTGGGC CAGACAGGGA CACGGCTCAC TCCAGCTGAC 
AAAGCAGAGC ACATGAAGCG ACAAAGACAC CCCAGATTGC GCCCCCAGTC AGCCCAGTCT 
TCTTTCCCTC CCTCCCCTGG TCCTTCTCCT GATGTGCAAC TGGCAACTCT GGCTCAGAGA 
GTCAAGGAAG TTTTGCCCCA TGTGCCATTG GGTGTCATCC AGAGAGACCT GGCCAAGACT 
GGCTGTGTAG ACTTGACTAT CACTAATCTG CTTGAGGGGG CCGTAGCTTT CATGCCTGAA 
GACATCACCA AGGGAACTCA GTCCCTACCC ACAGCCTCTG CCTCCAAGTT TCCCAGCTCT 
GGCCCGGTGA CCCCTCAGCC AACAGCCCTA ACATTTGCCA AGTCTTCCTG GGCCCGGCAG 
GAGAGCCTGC AGGAGCGCAA GCAAGCACTA TATGAATACG CAAGAAGGAG ATTCACAGAG 
AGACGAGCCC AGGAGGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

AUP1 (Ancient Ubiquitous Protein 1) Is a Key Determinant of Hepatic Very-Low-Density Lipoprotein Assembly and Secretion.
Arteriosclerosis, thrombosis, and vascular biology37(4)633-642(2017 Apr)
Zhang J,Zamani M,Thiele C,Taher J,Amir Alipour M,Yao Z,Adeli K

Identification of ERAD components essential for dislocation of the null Hong Kong variant of ?-1-antitrypsin (NHK).
Biochemical and biophysical research communications458(2)424-8(2015 Mar)
Zhong Y,Shen H,Wang Y,Yang Y,Yang P,Fang S

Monotopic topology is required for lipid droplet targeting of ancient ubiquitous protein 1.
Journal of lipid research54(2)503-13(2013 Feb)
Stevanovic A,Thiele C

MOR is not enough: identification of novel mu-opioid receptor interacting proteins using traditional and modified membrane yeast two-hybrid screens.
PloS one8(6)e67608(2013)
Petko J,Justice-Bitner S,Jin J,Wong V,Kittanakom S,Ferraro TN,Stagljar I,Levenson R

Monoubiquitination of ancient ubiquitous protein 1 promotes lipid droplet clustering.
PloS one8(9)e72453(2013)
Lohmann D,Spandl J,Stevanovic A,Schoene M,Philippou-Massier J,Thiele C

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AUP1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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