SDHAF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SDHAF2
Entrez Gene ID	54949
Full Name	succinate dehydrogenase complex assembly factor 2
Synonyms	C11orf79,PGL2,SDH5
General protein information	Preferred Names succinate dehydrogenase complex assembly factor 2 Names succinate dehydrogenase assembly factor 2, mitochondrial SDH assembly factor 2 hSDH5 succinate dehydrogenase subunit 5, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.2
Summary	This gene encodes a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. Mutations in this gene are associated with paraganglioma.[provided by RefSeq, Jul 2010].
Disorder MIM:	613019
Disorder Html:	Paragangliomas 2, 601650 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_017841.2	NP_060311.1	succinate dehydrogenase assembly factor 2, mitochondrial
NM_017841.4	NP_060311.1	succinate dehydrogenase assembly factor 2, mitochondrial

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sdhaf2	NP_001008372.1
Drosophila melanogaster (fruit fly)	CG14757	NP_610363.1
Drosophila melanogaster (fruit fly)	CG12895	NP_001260868.1
Homo sapiens (human)	SDHAF2	NP_060311.1
Eremothecium gossypii	AGOS_AER200C	NP_985057.1
Neurospora crassa	NCU01343	XP_961429.1
Danio rerio (zebrafish)	sdhaf2	NP_001076333.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012191	XP_320341.4
Kluyveromyces lactis	KLLA0E13333g	XP_454550.1
Pan troglodytes (chimpanzee)	PPP1R32	NP_001238783.1
	SDHAF2	XP_001082359.2
Canis lupus familiaris (dog)	SDHAF2	XP_533273.2
Mus musculus (house mouse)	Sdhaf2	NP_079609.2
Saccharomyces cerevisiae (baker's yeast)	EMI5	NP_014570.1
Xenopus tropicalis (tropical clawed frog)	sdhaf2	XP_002937676.1
Bos taurus (cattle)	SDHAF2	NP_001070513.1
Gallus gallus (chicken)	LOC426015	NP_001264818.1
Magnaporthe oryzae (rice blast fungus)	MGG_07034	XP_003715216.1

Gene Ontology
Bibliography

Related articles in PubMed

Clinical Characterization of the Pheochromocytoma and Paraganglioma Susceptibility Genes SDHA, TMEM127, MAX, and SDHAF2 for Gene-Informed Prevention.
Bausch B, Schiavi F, Ni Y, Welander J, Patocs A, Ngeow J, Wellner U, Malinoc A, Taschin E, Barbon G, Lanza V, S?derkvist P, Stenman A, Larsson C, Svahn F, Chen JL, Marquard J, Fraenkel M, Walter MA, Peczkowska M, Prejbisz A, Jarzab B, Hasse-Lazar K, Petersenn S, Moeller LC, Meyer A, Reisch N, Trupka A, Brase C, Galiano M, Preuss SF, Kwok P, Lendvai N, Berisha G, Makay ?, Boedeker CC, Weryha G, Racz K, Januszewicz A, Walz MK, Gimm O, Opocher G, Eng C, Neumann HPH,
JAMA oncology3(9)1204-1212(2017 Sep)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Loss of maternal chromosome 11 is a signature event in SDHAF2, SDHD, and VHL-related paragangliomas, but less significant in SDHB-related paragangliomas.
Hoekstra AS, Hensen EF, Jordanova ES, Korpershoek E, van der Horst-Schrivers AN, Cornelisse C, Corssmit EP, Hes FJ, Jansen JC, Kunst HP, Timmers HJ, Bateman A, Eccles D, Bov?e JV, Devilee P, Bayley JP
Oncotarget8(9)14525-14536(2017 Feb)

The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells.
Bezawork-Geleta A, Dong L, Rohlena J, Neuzil J
The Journal of biological chemistry291(41)21414-21420(2016 Oct)

In-vitro, SDH5-dependent flavinylation of immobilized human respiratory complex II flavoprotein.
Zafreen L, Walker-Kopp N, Huang LS, Berry E
Archives of biochemistry and biophysics60447-56(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The SDHA, TMEM127, MAX, and SDHAF2 genes contribute to hereditary pheochromocytoma and paraganglioma.
Title: Clinical Characterization of the Pheochromocytoma and Paraganglioma Susceptibility Genes SDHA, TMEM127, MAX, and SDHAF2 for Gene-Informed Prevention.

Loss of SDHAF2 gene is associated with paragangliomas.
Title: Loss of maternal chromosome 11 is a signature event in SDHAF2, SDHD, and VHL-related paragangliomas, but less significant in SDHB-related paragangliomas.

data show that SDHA flavination is independent of SDHAF2 in breast cancer cells, employing an alternative mechanism.
Title: The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells.

FAD interacts noncovalently with SDHA in the absence of SDH5
Title: In-vitro, SDH5-dependent flavinylation of immobilized human respiratory complex II flavoprotein.

Autosomal dominant susceptibility for Paraganglioma is modified by imprinting and mutations in the SDHAF2 gene cause Paragangliomas only when the mutation is inherited from father.
Title: A two-decade experience of head and neck paragangliomas in a whole population-based single centre cohort.

The following SDHAF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDHAF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22591	NM_017841.4 Latest version!	Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu22591	NM_017841.2	Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SDHAF2 ( NM_017841.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060311.1 Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA.

Price & Availability↑

CloneID	OHu22591
Clone ID Related Accession (Same CDS sequence)	NM_017841.2 , NM_017841.4
Accession Version	NM_017841.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	succinate dehydrogenase assembly factor 2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000494.1, AI039966.1 and AI660205.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced NM_017841.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000494.1, SRR1803615.273258.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGT CTACAGTGTT CTCGACTTCG TCGCTGATGC TTGCTCTGTC AAGGCACAGC 
CTATTGTCTC CTTTGCTCAG TGTGACATCA TTCAGACGCT TCTACAGAGG TGACAGCCCA 
ACAGATTCCC AAAAGGACAT GATTGAAATC CCTTTGCCTC CATGGCAGGA GAGAACTGAT 
GAATCCATAG AAACCAAAAG AGCCCGCCTG CTCTATGAGA GCAGAAAGAG GGGAATGTTG 
GAAAACTGCA TTCTTCTTAG TCTTTTTGCT AAAGAACATC TGCAGCACAT GACAGAAAAG 
CAGCTGAACC TCTATGACCG CCTGATTAAC GAGCCTAGTA ATGACTGGGA TATTTACTAC 
TGGGCCACAG AAGCTAAACC AGCCCCAGAA ATATTTGAAA ATGAAGTCAT GGCCCTGCTG 
AGAGACTTTG CTAAAAACAA AAACAAAGAG CAGAGACTGC GTGCCCCAGA TCTTGAGTAC 
CTCTTTGAAA AGCCACGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060311.1
CDS	23..523
Translation	MAVSTVFSTSSLMLALSRHSLLSPLLSVTSFRRFYRGDSPTDSQKDMIEIPLPPWQERTDESIETKRARLLYESRKRGMLENCILLSLFAKEHLQHMTEKQLNLYDRLINEPSNDWDIYYWATEAKPAPEIFENEVMALLRDFAKNKNKEQRLRAPDLEYLFEKPR

Target ORF information:

RefSeq Version	NM_017841.2
Organism	Homo sapiens(human)
Definition	Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017841.2

ORF Insert Sequence:

ATGGCGGTGT CTACAGTGTT CTCGACTTCG TCGCTGATGC TTGCTCTGTC AAGGCACAGC 
CTATTGTCTC CTTTGCTCAG TGTGACATCA TTCAGACGCT TCTACAGAGG TGACAGCCCA 
ACAGATTCCC AAAAGGACAT GATTGAAATC CCTTTGCCTC CATGGCAGGA GAGAACTGAT 
GAATCCATAG AAACCAAAAG AGCCCGCCTG CTCTATGAGA GCAGAAAGAG GGGAATGTTG 
GAAAACTGCA TTCTTCTTAG TCTTTTTGCT AAAGAACATC TGCAGCACAT GACAGAAAAG 
CAGCTGAACC TCTATGACCG CCTGATTAAC GAGCCTAGTA ATGACTGGGA TATTTACTAC 
TGGGCCACAG AAGCTAAACC AGCCCCAGAA ATATTTGAAA ATGAAGTCAT GGCCCTGCTG 
AGAGACTTTG CTAAAAACAA AAACAAAGAG CAGAGACTGC GTGCCCCAGA TCTTGAGTAC 
CTCTTTGAAA AGCCACGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SDHAF2 ( NM_017841.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060311.1 Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA.

Price & Availability↑

CloneID	OHu22591
Clone ID Related Accession (Same CDS sequence)	NM_017841.2 , NM_017841.4
Accession Version	NM_017841.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	succinate dehydrogenase assembly factor 2, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP003108.3. On Dec 3, 2019 this sequence version replaced NM_017841.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002331.2, BI544731.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGT CTACAGTGTT CTCGACTTCG TCGCTGATGC TTGCTCTGTC AAGGCACAGC 
CTATTGTCTC CTTTGCTCAG TGTGACATCA TTCAGACGCT TCTACAGAGG TGACAGCCCA 
ACAGATTCCC AAAAGGACAT GATTGAAATC CCTTTGCCTC CATGGCAGGA GAGAACTGAT 
GAATCCATAG AAACCAAAAG AGCCCGCCTG CTCTATGAGA GCAGAAAGAG GGGAATGTTG 
GAAAACTGCA TTCTTCTTAG TCTTTTTGCT AAAGAACATC TGCAGCACAT GACAGAAAAG 
CAGCTGAACC TCTATGACCG CCTGATTAAC GAGCCTAGTA ATGACTGGGA TATTTACTAC 
TGGGCCACAG AAGCTAAACC AGCCCCAGAA ATATTTGAAA ATGAAGTCAT GGCCCTGCTG 
AGAGACTTTG CTAAAAACAA AAACAAAGAG CAGAGACTGC GTGCCCCAGA TCTTGAGTAC 
CTCTTTGAAA AGCCACGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060311.1
CDS	24..524
Translation	MAVSTVFSTSSLMLALSRHSLLSPLLSVTSFRRFYRGDSPTDSQKDMIEIPLPPWQERTDESIETKRARLLYESRKRGMLENCILLSLFAKEHLQHMTEKQLNLYDRLINEPSNDWDIYYWATEAKPAPEIFENEVMALLRDFAKNKNKEQRLRAPDLEYLFEKPR

Target ORF information:

RefSeq Version	NM_017841.4
Organism	Homo sapiens(human)
Definition	Homo sapiens succinate dehydrogenase complex assembly factor 2 (SDHAF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017841.4

ORF Insert Sequence:

ATGGCGGTGT CTACAGTGTT CTCGACTTCG TCGCTGATGC TTGCTCTGTC AAGGCACAGC 
CTATTGTCTC CTTTGCTCAG TGTGACATCA TTCAGACGCT TCTACAGAGG TGACAGCCCA 
ACAGATTCCC AAAAGGACAT GATTGAAATC CCTTTGCCTC CATGGCAGGA GAGAACTGAT 
GAATCCATAG AAACCAAAAG AGCCCGCCTG CTCTATGAGA GCAGAAAGAG GGGAATGTTG 
GAAAACTGCA TTCTTCTTAG TCTTTTTGCT AAAGAACATC TGCAGCACAT GACAGAAAAG 
CAGCTGAACC TCTATGACCG CCTGATTAAC GAGCCTAGTA ATGACTGGGA TATTTACTAC 
TGGGCCACAG AAGCTAAACC AGCCCCAGAA ATATTTGAAA ATGAAGTCAT GGCCCTGCTG 
AGAGACTTTG CTAAAAACAA AAACAAAGAG CAGAGACTGC GTGCCCCAGA TCTTGAGTAC 
CTCTTTGAAA AGCCACGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical Characterization of the Pheochromocytoma and Paraganglioma Susceptibility Genes SDHA, TMEM127, MAX, and SDHAF2 for Gene-Informed Prevention.
JAMA oncology3(9)1204-1212(2017 Sep)
Bausch B,Schiavi F,Ni Y,Welander J,Patocs A,Ngeow J,Wellner U,Malinoc A,Taschin E,Barbon G,Lanza V,S?derkvist P,Stenman A,Larsson C,Svahn F,Chen JL,Marquard J,Fraenkel M,Walter MA,Peczkowska M,Prejbisz A,Jarzab B,Hasse-Lazar K,Petersenn S,Moeller LC,Meyer A,Reisch N,Trupka A,Brase C,Galiano M,Preuss SF,Kwok P,Lendvai N,Berisha G,Makay ?,Boedeker CC,Weryha G,Racz K,Januszewicz A,Walz MK,Gimm O,Opocher G,Eng C,Neumann HPH,

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Loss of maternal chromosome 11 is a signature event in SDHAF2, SDHD, and VHL-related paragangliomas, but less significant in SDHB-related paragangliomas.
Oncotarget8(9)14525-14536(2017 Feb)
Hoekstra AS,Hensen EF,Jordanova ES,Korpershoek E,van der Horst-Schrivers AN,Cornelisse C,Corssmit EP,Hes FJ,Jansen JC,Kunst HP,Timmers HJ,Bateman A,Eccles D,Bov?e JV,Devilee P,Bayley JP

The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells.
The Journal of biological chemistry291(41)21414-21420(2016 Oct)
Bezawork-Geleta A,Dong L,Rohlena J,Neuzil J

In-vitro, SDH5-dependent flavinylation of immobilized human respiratory complex II flavoprotein.
Archives of biochemistry and biophysics60447-56(2016 Aug)
Zafreen L,Walker-Kopp N,Huang LS,Berry E

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SDHAF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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