PNLIP cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PNLIP
Entrez Gene ID	5406
Full Name	pancreatic lipase
Synonyms	PL,PNLIPD,PTL
General protein information	Preferred Names pancreatic lipase Names pancreatic triacylglycerol lipase triacylglycerol acylhydrolase
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 10 Map Location: 10q25.3
Summary	This gene encodes a member of the lipase family of proteins. The encoded enzyme is secreted by the pancreas and hydrolyzes triglycerides in the small intestine, and is essential for the efficient digestion of dietary fats. Inhibition of the encoded enzyme may prevent high-fat diet-induced obesity in mice and result in weight loss in human patients with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by steatorrhea. [provided by RefSeq, Jul 2016].
Disorder MIM:	246600
Disorder Html:	?Pancreatic lipase deficiency, 614338 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000936.3	NP_000927.1	pancreatic triacylglycerol lipase precursor
NM_000936.4	NP_000927.1	pancreatic triacylglycerol lipase precursor
NM_000936.2	NP_000927.1	pancreatic triacylglycerol lipase precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PNLIP	XP_001151144.1
Canis lupus familiaris (dog)	PNLIP	XP_535023.2
Bos taurus (cattle)	PNLIP	NP_001192749.1
Mus musculus (house mouse)	Pnlip	NP_081201.2
Drosophila melanogaster (fruit fly)	CG5966	NP_572286.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000211	XP_310922.4
	PNLIP	XP_001095070.1
Homo sapiens (human)	PNLIP	NP_000927.1
Rattus norvegicus (Norway rat)	Pnlip	NP_037293.1

Gene Ontology
Bibliography

Related articles in PubMed

Clinical efficacy of serum lipase subtype analysis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation.
Bang CS, Kim JB, Park SH, Baik GH, Su KT, Yoon JH, Kim YS, Kim DJ
The Korean journal of internal medicine31(4)660-8(2016 Jul)

A novel mutation in PNLIP causes pancreatic triglyceride lipase deficiency through protein misfolding.
Szab? A, Xiao X, Haughney M, Spector A, Sahin-T?th M, Lowe ME
Biochimica et biophysica acta1852(7)1372-9(2015 Jul)

A broad pH range indicator-based spectrophotometric assay for true lipases using tributyrin and tricaprylin.
Camacho-Ruiz Mde L, Mateos-D?az JC, Carri?re F, Rodriguez JA
Journal of lipid research56(5)1057-67(2015 May)

Abnormal serum pancreatic enzymes, but not pancreatitis, are associated with an increased risk of malignancy in patients with intraductal papillary mucinous neoplasms.
Roch AM, Parikh JA, Al-Haddad MA, DeWitt JM, Ceppa EP, House MG, Nakeeb A, Schmidt CM
Surgery156(4)923-9(2014 Oct)

An interfacial and comparative in vitro study of gastrointestinal lipases and Yarrowia lipolytica LIP2 lipase, a candidate for enzyme replacement therapy.
B?narouche A, Point V, Carri?re F, Cavalier JF
Biochimie102145-53(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Serum lipase subtype analysis cannot replace the standard lipase measurement for the diagnosis of acute pancreatitis, but the test demonstrated adequate sensitivity for use in triage or as an add-on test for serum lipase elevation.
Title: Clinical efficacy of serum lipase subtype analysis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation.

Presence of methionine at position 221 in the PNLIP protein sequence causes protein misfolding and aggregation.
Title: A novel mutation in PNLIP causes pancreatic triglyceride lipase deficiency through protein misfolding.

In patients with intraductal papillary mucinous neoplasm without a history of pancreatitis, serum pancreatic lipase and amylase outside normal range are associated with risk of malignancy.
Title: Abnormal serum pancreatic enzymes, but not pancreatitis, are associated with an increased risk of malignancy in patients with intraductal papillary mucinous neoplasms.

All these findings support the use of YLLIP2 in enzyme replacement therapy for the treatment of pancreatic exocrine insufficiency, a pathological situation in which an acidification of intestinal contents occurs.
Title: An interfacial and comparative in vitro study of gastrointestinal lipases and Yarrowia lipolytica LIP2 lipase, a candidate for enzyme replacement therapy.

a novel mutation in the PNLIP gene in two brothers with congenital pancreatic lipase deficiency
Title: Identification of a novel mutation in the PNLIP gene in two brothers with congenital pancreatic lipase deficiency.

The following PNLIP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNLIP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18255	NM_000936.3 Latest version!	Homo sapiens pancreatic lipase (PNLIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$194.50-$272.30 $389.00
OHu107359	NM_000936.4 Latest version!	Homo sapiens pancreatic lipase (PNLIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu18255	NM_000936.2	Homo sapiens pancreatic lipase (PNLIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$194.50-$272.30 $389.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PNLIP ( NM_000936.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000927.1 Homo sapiens pancreatic lipase (PNLIP), mRNA.

Price & Availability↑

CloneID	OHu18255
Clone ID Related Accession (Same CDS sequence)	NM_000936.3 , NM_000936.2
Accession Version	NM_000936.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Homo sapiens(human)
Product	pancreatic triacylglycerol lipase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL731653.1, J05125.1 and AW873921.1. This sequence is a reference standard in the RefSeqGene project. On Jul 8, 2016 this sequence version replaced NM_000936.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05125.1, AK313941.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTGCCAC TTTGGACTCT TTCACTGCTG CTGGGAGCAG TAGCAGGAAA AGAAGTTTGC 
TACGAAAGAC TCGGCTGCTT CAGTGATGAC TCCCCATGGT CAGGAATTAC GGAAAGACCC 
CTCCATATAT TGCCTTGGTC TCCAAAAGAT GTCAACACCC GCTTCCTCCT ATATACTAAT 
GAGAACCCAA ACAACTTTCA AGAAGTTGCC GCAGATTCAT CAAGCATCAG TGGCTCCAAT 
TTCAAAACAA ATAGAAAAAC TCGCTTTATT ATTCATGGAT TCATAGACAA GGGAGAAGAA 
AACTGGCTGG CCAATGTGTG CAAGAATCTG TTCAAGGTGG AAAGTGTGAA CTGTATCTGT 
GTGGACTGGA AAGGTGGCTC CCGAACTGGA TACACACAAG CCTCGCAGAA CATCAGGATC 
GTGGGAGCAG AAGTGGCATA TTTTGTTGAA TTTCTTCAGT CGGCGTTCGG TTACTCACCT 
TCCAACGTGC ATGTCATTGG CCACAGCCTG GGTGCCCACG CTGCTGGGGA GGCTGGAAGG 
AGAACCAATG GGACCATTGG ACGCATCACA GGGTTGGACC CAGCAGAACC TTGCTTTCAG 
GGCACACCTG AATTAGTCCG ATTGGACCCC AGCGATGCCA AATTTGTGGA TGTAATTCAC 
ACGGATGGTG CCCCCATAGT CCCCAATTTG GGGTTTGGAA TGAGCCAAGT CGTGGGCCAC 
CTAGATTTCT TTCCAAATGG AGGAGTGGAA ATGCCTGGAT GTAAAAAGAA CATTCTCTCT 
CAGATTGTGG ACATAGACGG AATCTGGGAA GGGACTCGAG ACTTTGCGGC CTGTAATCAC 
TTAAGAAGCT ACAAATATTA CACTGATAGC ATCGTCAACC CTGATGGCTT TGCTGGATTC 
CCCTGTGCCT CTTACAACGT CTTCACTGCA AACAAGTGTT TCCCTTGTCC AAGTGGAGGC 
TGCCCACAGA TGGGTCACTA TGCTGATAGA TATCCTGGGA AAACAAATGA TGTGGGCCAG 
AAATTTTATC TAGACACTGG TGATGCCAGT AATTTTGCAC GTTGGAGGTA TAAGGTATCT 
GTCACACTGT CTGGAAAAAA GGTTACAGGA CACATACTAG TTTCTTTGTT CGGAAATAAA 
GGAAACTCTA AGCAGTATGA AATTTTCAAG GGCACTCTCA AACCAGATAG TACTCATTCC 
AATGAATTTG ACTCAGATGT GGATGTTGGG GACTTGCAGA TGGTTAAATT TATTTGGTAT 
AACAATGTGA TCAACCCAAC TTTACCTAGA GTGGGAGCAT CCAAGATTAT AGTGGAGACA 
AATGTTGGAA AACAGTTCAA CTTCTGTAGT CCAGAAACCG TCAGGGAGGA AGTTCTGCTC 
ACCCTCACAC CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000927.1
CDS	36..1433
Translation	MLPLWTLSLLLGAVAGKEVCYERLGCFSDDSPWSGITERPLHILPWSPKDVNTRFLLYTNENPNNFQEVAADSSSISGSNFKTNRKTRFIIHGFIDKGEENWLANVCKNLFKVESVNCICVDWKGGSRTGYTQASQNIRIVGAEVAYFVEFLQSAFGYSPSNVHVIGHSLGAHAAGEAGRRTNGTIGRITGLDPAEPCFQGTPELVRLDPSDAKFVDVIHTDGAPIVPNLGFGMSQVVGHLDFFPNGGVEMPGCKKNILSQIVDIDGIWEGTRDFAACNHLRSYKYYTDSIVNPDGFAGFPCASYNVFTANKCFPCPSGGCPQMGHYADRYPGKTNDVGQKFYLDTGDASNFARWRYKVSVTLSGKKVTGHILVSLFGNKGNSKQYEIFKGTLKPDSTHSNEFDSDVDVGDLQMVKFIWYNNVINPTLPRVGASKIIVETNVGKQFNFCSPETVREEVLLTLTPC

Target ORF information:

RefSeq Version	NM_000936.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pancreatic lipase (PNLIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000936.3

ORF Insert Sequence:

ATGCTGCCAC TTTGGACTCT TTCACTGCTG CTGGGAGCAG TAGCAGGAAA AGAAGTTTGC 
TACGAAAGAC TCGGCTGCTT CAGTGATGAC TCCCCATGGT CAGGAATTAC GGAAAGACCC 
CTCCATATAT TGCCTTGGTC TCCAAAAGAT GTCAACACCC GCTTCCTCCT ATATACTAAT 
GAGAACCCAA ACAACTTTCA AGAAGTTGCC GCAGATTCAT CAAGCATCAG TGGCTCCAAT 
TTCAAAACAA ATAGAAAAAC TCGCTTTATT ATTCATGGAT TCATAGACAA GGGAGAAGAA 
AACTGGCTGG CCAATGTGTG CAAGAATCTG TTCAAGGTGG AAAGTGTGAA CTGTATCTGT 
GTGGACTGGA AAGGTGGCTC CCGAACTGGA TACACACAAG CCTCGCAGAA CATCAGGATC 
GTGGGAGCAG AAGTGGCATA TTTTGTTGAA TTTCTTCAGT CGGCGTTCGG TTACTCACCT 
TCCAACGTGC ATGTCATTGG CCACAGCCTG GGTGCCCACG CTGCTGGGGA GGCTGGAAGG 
AGAACCAATG GGACCATTGG ACGCATCACA GGGTTGGACC CAGCAGAACC TTGCTTTCAG 
GGCACACCTG AATTAGTCCG ATTGGACCCC AGCGATGCCA AATTTGTGGA TGTAATTCAC 
ACGGATGGTG CCCCCATAGT CCCCAATTTG GGGTTTGGAA TGAGCCAAGT CGTGGGCCAC 
CTAGATTTCT TTCCAAATGG AGGAGTGGAA ATGCCTGGAT GTAAAAAGAA CATTCTCTCT 
CAGATTGTGG ACATAGACGG AATCTGGGAA GGGACTCGAG ACTTTGCGGC CTGTAATCAC 
TTAAGAAGCT ACAAATATTA CACTGATAGC ATCGTCAACC CTGATGGCTT TGCTGGATTC 
CCCTGTGCCT CTTACAACGT CTTCACTGCA AACAAGTGTT TCCCTTGTCC AAGTGGAGGC 
TGCCCACAGA TGGGTCACTA TGCTGATAGA TATCCTGGGA AAACAAATGA TGTGGGCCAG 
AAATTTTATC TAGACACTGG TGATGCCAGT AATTTTGCAC GTTGGAGGTA TAAGGTATCT 
GTCACACTGT CTGGAAAAAA GGTTACAGGA CACATACTAG TTTCTTTGTT CGGAAATAAA 
GGAAACTCTA AGCAGTATGA AATTTTCAAG GGCACTCTCA AACCAGATAG TACTCATTCC 
AATGAATTTG ACTCAGATGT GGATGTTGGG GACTTGCAGA TGGTTAAATT TATTTGGTAT 
AACAATGTGA TCAACCCAAC TTTACCTAGA GTGGGAGCAT CCAAGATTAT AGTGGAGACA 
AATGTTGGAA AACAGTTCAA CTTCTGTAGT CCAGAAACCG TCAGGGAGGA AGTTCTGCTC 
ACCCTCACAC CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNLIP ( NM_000936.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000927.1 Homo sapiens pancreatic lipase (PNLIP), mRNA.

Price & Availability↑

CloneID	OHu18255
Clone ID Related Accession (Same CDS sequence)	NM_000936.3 , NM_000936.2
Accession Version	NM_000936.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	pancreatic triacylglycerol lipase precursor
Comment

ATGCTGCCAC TTTGGACTCT TTCACTGCTG CTGGGAGCAG TAGCAGGAAA AGAAGTTTGC 
TACGAAAGAC TCGGCTGCTT CAGTGATGAC TCCCCATGGT CAGGAATTAC GGAAAGACCC 
CTCCATATAT TGCCTTGGTC TCCAAAAGAT GTCAACACCC GCTTCCTCCT ATATACTAAT 
GAGAACCCAA ACAACTTTCA AGAAGTTGCC GCAGATTCAT CAAGCATCAG TGGCTCCAAT 
TTCAAAACAA ATAGAAAAAC TCGCTTTATT ATTCATGGAT TCATAGACAA GGGAGAAGAA 
AACTGGCTGG CCAATGTGTG CAAGAATCTG TTCAAGGTGG AAAGTGTGAA CTGTATCTGT 
GTGGACTGGA AAGGTGGCTC CCGAACTGGA TACACACAAG CCTCGCAGAA CATCAGGATC 
GTGGGAGCAG AAGTGGCATA TTTTGTTGAA TTTCTTCAGT CGGCGTTCGG TTACTCACCT 
TCCAACGTGC ATGTCATTGG CCACAGCCTG GGTGCCCACG CTGCTGGGGA GGCTGGAAGG 
AGAACCAATG GGACCATTGG ACGCATCACA GGGTTGGACC CAGCAGAACC TTGCTTTCAG 
GGCACACCTG AATTAGTCCG ATTGGACCCC AGCGATGCCA AATTTGTGGA TGTAATTCAC 
ACGGATGGTG CCCCCATAGT CCCCAATTTG GGGTTTGGAA TGAGCCAAGT CGTGGGCCAC 
CTAGATTTCT TTCCAAATGG AGGAGTGGAA ATGCCTGGAT GTAAAAAGAA CATTCTCTCT 
CAGATTGTGG ACATAGACGG AATCTGGGAA GGGACTCGAG ACTTTGCGGC CTGTAATCAC 
TTAAGAAGCT ACAAATATTA CACTGATAGC ATCGTCAACC CTGATGGCTT TGCTGGATTC 
CCCTGTGCCT CTTACAACGT CTTCACTGCA AACAAGTGTT TCCCTTGTCC AAGTGGAGGC 
TGCCCACAGA TGGGTCACTA TGCTGATAGA TATCCTGGGA AAACAAATGA TGTGGGCCAG 
AAATTTTATC TAGACACTGG TGATGCCAGT AATTTTGCAC GTTGGAGGTA TAAGGTATCT 
GTCACACTGT CTGGAAAAAA GGTTACAGGA CACATACTAG TTTCTTTGTT CGGAAATAAA 
GGAAACTCTA AGCAGTATGA AATTTTCAAG GGCACTCTCA AACCAGATAG TACTCATTCC 
AATGAATTTG ACTCAGATGT GGATGTTGGG GACTTGCAGA TGGTTAAATT TATTTGGTAT 
AACAATGTGA TCAACCCAAC TTTACCTAGA GTGGGAGCAT CCAAGATTAT AGTGGAGACA 
AATGTTGGAA AACAGTTCAA CTTCTGTAGT CCAGAAACCG TCAGGGAGGA AGTTCTGCTC 
ACCCTCACAC CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_000927.1

Translation

MLPLWTLSLLLGAVAGKEVCYERLGCFSDDSPWSGITERPLHILPWSPKDVNTRFLLYTNENPNNFQEVAADSSSISGSNFKTNRKTRFIIHGFIDKGEENWLANVCKNLFKVESVNCICVDWKGGSRTGYTQASQNIRIVGAEVAYFVEFLQSAFGYSPSNVHVIGHSLGAHAAGEAGRRTNGTIGRITGLDPAEPCFQGTPELVRLDPSDAKFVDVIHTDGAPIVPNLGFGMSQVVGHLDFFPNGGVEMPGCKKNILSQIVDIDGIWEGTRDFAACNHLRSYKYYTDSIVNPDGFAGFPCASYNVFTANKCFPCPSGGCPQMGHYADRYPGKTNDVGQKFYLDTGDASNFARWRYKVSVTLSGKKVTGHILVSLFGNKGNSKQYEIFKGTLKPDSTHSNEFDSDVDVGDLQMVKFIWYNNVINPTLPRVGASKIIVETNVGKQFNFCSPETVREEVLLTLTPC

Target ORF information:

RefSeq Version	NM_000936.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens pancreatic lipase (PNLIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000936.2

ORF Insert Sequence:

ATGCTGCCAC TTTGGACTCT TTCACTGCTG CTGGGAGCAG TAGCAGGAAA AGAAGTTTGC 
TACGAAAGAC TCGGCTGCTT CAGTGATGAC TCCCCATGGT CAGGAATTAC GGAAAGACCC 
CTCCATATAT TGCCTTGGTC TCCAAAAGAT GTCAACACCC GCTTCCTCCT ATATACTAAT 
GAGAACCCAA ACAACTTTCA AGAAGTTGCC GCAGATTCAT CAAGCATCAG TGGCTCCAAT 
TTCAAAACAA ATAGAAAAAC TCGCTTTATT ATTCATGGAT TCATAGACAA GGGAGAAGAA 
AACTGGCTGG CCAATGTGTG CAAGAATCTG TTCAAGGTGG AAAGTGTGAA CTGTATCTGT 
GTGGACTGGA AAGGTGGCTC CCGAACTGGA TACACACAAG CCTCGCAGAA CATCAGGATC 
GTGGGAGCAG AAGTGGCATA TTTTGTTGAA TTTCTTCAGT CGGCGTTCGG TTACTCACCT 
TCCAACGTGC ATGTCATTGG CCACAGCCTG GGTGCCCACG CTGCTGGGGA GGCTGGAAGG 
AGAACCAATG GGACCATTGG ACGCATCACA GGGTTGGACC CAGCAGAACC TTGCTTTCAG 
GGCACACCTG AATTAGTCCG ATTGGACCCC AGCGATGCCA AATTTGTGGA TGTAATTCAC 
ACGGATGGTG CCCCCATAGT CCCCAATTTG GGGTTTGGAA TGAGCCAAGT CGTGGGCCAC 
CTAGATTTCT TTCCAAATGG AGGAGTGGAA ATGCCTGGAT GTAAAAAGAA CATTCTCTCT 
CAGATTGTGG ACATAGACGG AATCTGGGAA GGGACTCGAG ACTTTGCGGC CTGTAATCAC 
TTAAGAAGCT ACAAATATTA CACTGATAGC ATCGTCAACC CTGATGGCTT TGCTGGATTC 
CCCTGTGCCT CTTACAACGT CTTCACTGCA AACAAGTGTT TCCCTTGTCC AAGTGGAGGC 
TGCCCACAGA TGGGTCACTA TGCTGATAGA TATCCTGGGA AAACAAATGA TGTGGGCCAG 
AAATTTTATC TAGACACTGG TGATGCCAGT AATTTTGCAC GTTGGAGGTA TAAGGTATCT 
GTCACACTGT CTGGAAAAAA GGTTACAGGA CACATACTAG TTTCTTTGTT CGGAAATAAA 
GGAAACTCTA AGCAGTATGA AATTTTCAAG GGCACTCTCA AACCAGATAG TACTCATTCC 
AATGAATTTG ACTCAGATGT GGATGTTGGG GACTTGCAGA TGGTTAAATT TATTTGGTAT 
AACAATGTGA TCAACCCAAC TTTACCTAGA GTGGGAGCAT CCAAGATTAT AGTGGAGACA 
AATGTTGGAA AACAGTTCAA CTTCTGTAGT CCAGAAACCG TCAGGGAGGA AGTTCTGCTC 
ACCCTCACAC CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PNLIP ( NM_000936.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000927.1 Homo sapiens pancreatic lipase (PNLIP), mRNA.

Price & Availability↑

CloneID	OHu107359
Clone ID Related Accession (Same CDS sequence)	NM_000936.4
Accession Version	NM_000936.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	pancreatic triacylglycerol lipase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL731653.1. On Nov 23, 2018 this sequence version replaced NM_000936.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.242809.1, SRR5189667.132593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369221.2/ ENSP00000358223.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCCAC TTTGGACTCT TTCACTGCTG CTGGGAGCAG TAGCAGGAAA AGAAGTTTGC 
TACGAAAGAC TCGGCTGCTT CAGTGATGAC TCCCCATGGT CAGGAATTAC GGAAAGACCC 
CTCCATATAT TGCCTTGGTC TCCAAAAGAT GTCAACACCC GCTTCCTCCT ATATACTAAT 
GAGAACCCAA ACAACTTTCA AGAAGTTGCC GCAGATTCAT CAAGCATCAG TGGCTCCAAT 
TTCAAAACAA ATAGAAAAAC TCGCTTTATT ATTCATGGAT TCATAGACAA GGGAGAAGAA 
AACTGGCTGG CCAATGTGTG CAAGAATCTG TTCAAGGTGG AAAGTGTGAA CTGTATCTGT 
GTGGACTGGA AAGGTGGCTC CCGAACTGGA TACACACAAG CCTCGCAGAA CATCAGGATC 
GTGGGAGCAG AAGTGGCATA TTTTGTTGAA TTTCTTCAGT CGGCGTTCGG TTACTCACCT 
TCCAATGTGC ATGTCATTGG CCACAGCCTG GGTGCCCACG CTGCTGGGGA GGCTGGAAGG 
AGAACCAATG GGACCATTGG ACGCATCACA GGGTTGGACC CAGCAGAACC TTGCTTTCAG 
GGCACACCTG AATTAGTCCG ATTGGACCCC AGCGATGCCA AATTTGTGGA TGTAATTCAC 
ACGGATGGTG CCCCCATAGT CCCCAATTTG GGGTTTGGAA TGAGCCAAGT CGTGGGCCAC 
CTAGATTTCT TTCCAAATGG AGGAGTGGAA ATGCCTGGAT GTAAAAAGAA CATTCTCTCT 
CAGATTGTGG ACATAGACGG AATCTGGGAA GGGACTCGAG ACTTTGCGGC CTGTAATCAC 
TTAAGAAGCT ACAAATATTA CACTGATAGC ATCGTCAACC CTGATGGCTT TGCTGGATTC 
CCCTGTGCCT CTTACAACGT CTTCACTGCA AACAAGTGTT TCCCTTGTCC AAGTGGAGGC 
TGCCCACAGA TGGGTCACTA TGCTGATAGA TATCCTGGGA AAACAAATGA TGTGGGCCAG 
AAATTTTATC TAGACACTGG TGATGCCAGT AATTTTGCAC GTTGGAGGTA TAAGGTATCT 
GTCACACTGT CTGGAAAAAA GGTTACAGGA CACATACTAG TTTCTTTGTT CGGAAATAAA 
GGAAACTCTA AGCAGTATGA AATTTTCAAG GGCACTCTCA AACCAGATAG TACTCATTCC 
AATGAATTTG ACTCAGATGT GGATGTTGGG GACTTGCAGA TGGTTAAATT TATTTGGTAT 
AACAATGTGA TCAACCCAAC TTTACCTAGA GTGGGAGCAT CCAAGATTAT AGTGGAGACA 
AATGTTGGAA AACAGTTCAA CTTCTGTAGT CCAGAAACCG TCAGGGAGGA AGTTCTGCTC 
ACCCTCACAC CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000927.1
CDS	29..1426
Translation	MLPLWTLSLLLGAVAGKEVCYERLGCFSDDSPWSGITERPLHILPWSPKDVNTRFLLYTNENPNNFQEVAADSSSISGSNFKTNRKTRFIIHGFIDKGEENWLANVCKNLFKVESVNCICVDWKGGSRTGYTQASQNIRIVGAEVAYFVEFLQSAFGYSPSNVHVIGHSLGAHAAGEAGRRTNGTIGRITGLDPAEPCFQGTPELVRLDPSDAKFVDVIHTDGAPIVPNLGFGMSQVVGHLDFFPNGGVEMPGCKKNILSQIVDIDGIWEGTRDFAACNHLRSYKYYTDSIVNPDGFAGFPCASYNVFTANKCFPCPSGGCPQMGHYADRYPGKTNDVGQKFYLDTGDASNFARWRYKVSVTLSGKKVTGHILVSLFGNKGNSKQYEIFKGTLKPDSTHSNEFDSDVDVGDLQMVKFIWYNNVINPTLPRVGASKIIVETNVGKQFNFCSPETVREEVLLTLTPC

Target ORF information:

RefSeq Version	NM_000936.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pancreatic lipase (PNLIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000936.4

ORF Insert Sequence:

ATGCTGCCAC TTTGGACTCT TTCACTGCTG CTGGGAGCAG TAGCAGGAAA AGAAGTTTGC 
TACGAAAGAC TCGGCTGCTT CAGTGATGAC TCCCCATGGT CAGGAATTAC GGAAAGACCC 
CTCCATATAT TGCCTTGGTC TCCAAAAGAT GTCAACACCC GCTTCCTCCT ATATACTAAT 
GAGAACCCAA ACAACTTTCA AGAAGTTGCC GCAGATTCAT CAAGCATCAG TGGCTCCAAT 
TTCAAAACAA ATAGAAAAAC TCGCTTTATT ATTCATGGAT TCATAGACAA GGGAGAAGAA 
AACTGGCTGG CCAATGTGTG CAAGAATCTG TTCAAGGTGG AAAGTGTGAA CTGTATCTGT 
GTGGACTGGA AAGGTGGCTC CCGAACTGGA TACACACAAG CCTCGCAGAA CATCAGGATC 
GTGGGAGCAG AAGTGGCATA TTTTGTTGAA TTTCTTCAGT CGGCGTTCGG TTACTCACCT 
TCCAATGTGC ATGTCATTGG CCACAGCCTG GGTGCCCACG CTGCTGGGGA GGCTGGAAGG 
AGAACCAATG GGACCATTGG ACGCATCACA GGGTTGGACC CAGCAGAACC TTGCTTTCAG 
GGCACACCTG AATTAGTCCG ATTGGACCCC AGCGATGCCA AATTTGTGGA TGTAATTCAC 
ACGGATGGTG CCCCCATAGT CCCCAATTTG GGGTTTGGAA TGAGCCAAGT CGTGGGCCAC 
CTAGATTTCT TTCCAAATGG AGGAGTGGAA ATGCCTGGAT GTAAAAAGAA CATTCTCTCT 
CAGATTGTGG ACATAGACGG AATCTGGGAA GGGACTCGAG ACTTTGCGGC CTGTAATCAC 
TTAAGAAGCT ACAAATATTA CACTGATAGC ATCGTCAACC CTGATGGCTT TGCTGGATTC 
CCCTGTGCCT CTTACAACGT CTTCACTGCA AACAAGTGTT TCCCTTGTCC AAGTGGAGGC 
TGCCCACAGA TGGGTCACTA TGCTGATAGA TATCCTGGGA AAACAAATGA TGTGGGCCAG 
AAATTTTATC TAGACACTGG TGATGCCAGT AATTTTGCAC GTTGGAGGTA TAAGGTATCT 
GTCACACTGT CTGGAAAAAA GGTTACAGGA CACATACTAG TTTCTTTGTT CGGAAATAAA 
GGAAACTCTA AGCAGTATGA AATTTTCAAG GGCACTCTCA AACCAGATAG TACTCATTCC 
AATGAATTTG ACTCAGATGT GGATGTTGGG GACTTGCAGA TGGTTAAATT TATTTGGTAT 
AACAATGTGA TCAACCCAAC TTTACCTAGA GTGGGAGCAT CCAAGATTAT AGTGGAGACA 
AATGTTGGAA AACAGTTCAA CTTCTGTAGT CCAGAAACCG TCAGGGAGGA AGTTCTGCTC 
ACCCTCACAC CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical efficacy of serum lipase subtype analysis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation.
The Korean journal of internal medicine31(4)660-8(2016 Jul)
Bang CS,Kim JB,Park SH,Baik GH,Su KT,Yoon JH,Kim YS,Kim DJ

A novel mutation in PNLIP causes pancreatic triglyceride lipase deficiency through protein misfolding.
Biochimica et biophysica acta1852(7)1372-9(2015 Jul)
Szab? A,Xiao X,Haughney M,Spector A,Sahin-T?th M,Lowe ME

A broad pH range indicator-based spectrophotometric assay for true lipases using tributyrin and tricaprylin.
Journal of lipid research56(5)1057-67(2015 May)
Camacho-Ruiz Mde L,Mateos-D?az JC,Carri?re F,Rodriguez JA

Abnormal serum pancreatic enzymes, but not pancreatitis, are associated with an increased risk of malignancy in patients with intraductal papillary mucinous neoplasms.
Surgery156(4)923-9(2014 Oct)
Roch AM,Parikh JA,Al-Haddad MA,DeWitt JM,Ceppa EP,House MG,Nakeeb A,Schmidt CM

An interfacial and comparative in vitro study of gastrointestinal lipases and Yarrowia lipolytica LIP2 lipase, a candidate for enzyme replacement therapy.
Biochimie102145-53(2014 Jul)
B?narouche A,Point V,Carri?re F,Cavalier JF

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PNLIP cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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