PFDN5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFDN5
Entrez Gene ID	5204
Full Name	prefoldin subunit 5
Synonyms	MM-1,MM1,PFD5
General protein information	Preferred Names prefoldin subunit 5 Names prefoldin subunit 5 c-myc binding protein myc modulator-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.13
Summary	This gene encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:	604899

mRNA and Protein(s)

mRNA	Protein	Name
NM_145897.2	NP_665904.1	prefoldin subunit 5 isoform gamma
NM_002624.3	NP_002615.2	prefoldin subunit 5 isoform alpha
NM_002624.4	NP_002615.2	prefoldin subunit 5 isoform alpha

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pfdn5	NP_081320.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003416	XP_311700.3
Eremothecium gossypii	AGOS_ADL352C	NP_983743.2
Rattus norvegicus (Norway rat)	Pfdn5	NP_001100264.2
Saccharomyces cerevisiae (baker's yeast)	GIM5	NP_013616.1
Kluyveromyces lactis	KLLA0D10329g	XP_453521.1
Oryza sativa (rice)	Os10g0488100	NP_001064910.1
	PFDN5	XP_001104576.1
Canis lupus familiaris (dog)	PFDN5	NP_001238878.1
Bos taurus (cattle)	PFDN5	NP_777157.1
Magnaporthe oryzae (rice blast fungus)	MGG_14848	XP_003717239.1
Homo sapiens (human)	PFDN5	NP_002615.2
Pan troglodytes (chimpanzee)	PFDN5	XP_509097.2
Danio rerio (zebrafish)	pfdn5	NP_001104665.1
Arabidopsis thaliana (thale cress)	PFD5	NP_197720.1
Gallus gallus (chicken)	LOC100859376	XP_003643470.1
Drosophila melanogaster (fruit fly)	CG7048	NP_001163688.1
Schizosaccharomyces pombe (fission yeast)	bob1	NP_596679.1
Neurospora crassa	NCU02963	XP_965350.1
Xenopus tropicalis (tropical clawed frog)	pfdn5	NP_001016185.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
Hasegawa Y, Taylor D, Ovchinnikov DA, Wolvetang EJ, de Torrent? L, Mar JC
PLoS genetics11(8)e1005428(2015 Aug)

A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways.
Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, Choi H, Berger B, Gingras AC, Lindquist S
Cell158(2)434-448(2014 Jul)

Prefoldin protects neuronal cells from polyglutamine toxicity by preventing aggregation formation.
Tashiro E, Zako T, Muto H, Itoo Y, S?rgjerd K, Terada N, Abe A, Miyazawa M, Kitamura A, Kitaura H, Kubota H, Maeda M, Momoi T, Iguchi-Ariga SM, Kinjo M, Ariga H
The Journal of biological chemistry288(27)19958-72(2013 Jul)

Rabring7 degrades c-Myc through complex formation with MM-1.
Narita R, Kitaura H, Torii A, Tashiro E, Miyazawa M, Ariga H, Iguchi-Ariga SM
PloS one7(7)e41891(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

knockdown of PFD2 and PFD5 disrupted prefoldin formation in HTT-expressing cells, resulting in accumulation of aggregates of a pathogenic form of HTT and in induction of cell death.
Title: Prefoldin protects neuronal cells from polyglutamine toxicity by preventing aggregation formation.

Rabring7 stimulates c-Myc degradation via mono-ubiquitination of MM-1
Title: Rabring7 degrades c-Myc through complex formation with MM-1.

MM-1, a c-Myc interacting protein, interacts with HCV ARFP/F protein, which can result in the enhancement of the c-Myc activity.
Title: Hepatitis C virus ARFP/F protein interacts with cellular MM-1 protein and enhances the gene trans-activation activity of c-Myc.

Wnt4 gene, a member of the Wnt-beta-catenin pathway, was identified as a target gene of MM-1.
Title: Negative regulation of the Wnt signal by MM-1 through inhibiting expression of the wnt4 gene.

MM-1alpha, MM-1gamma, and MM-1gamma, but not MM-1beta, were found to repress transcription activity of c-Myc
Title: Distinct localizations and repression activities of MM-1 isoforms toward c-Myc.

The following PFDN5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFDN5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19320	NM_002624.4 Latest version!	Homo sapiens prefoldin subunit 5 (PFDN5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu30182	NM_145897.2 Latest version!	Homo sapiens prefoldin subunit 5 (PFDN5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu19320	NM_002624.3	Homo sapiens prefoldin subunit 5 (PFDN5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFDN5 ( NM_002624.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002615.2 Homo sapiens prefoldin subunit 5 (PFDN5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19320
Clone ID Related Accession (Same CDS sequence)	NM_002624.3 , NM_002624.4
Accession Version	NM_002624.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	prefoldin subunit 5 isoform alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE392248.1, AB055803.1 and AB055805.1. This sequence is a reference standard in the RefSeqGene project. On Feb 28, 2006 this sequence version replaced NM_002624.2. Transcript Variant: This variant (1) encodes the longest isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.418136.1, BG707826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGT CTATTAACAT CACGGAGCTG AATCTGCCGC AGCTAGAAAT GCTCAAGAAC 
CAGCTGGACC AGGAAGTGGA GTTCTTGTCC ACGTCCATTG CTCAGCTCAA AGTGGTACAG 
ACCAAGTATG TGGAAGCCAA GGACTGTCTG AACGTGCTGA ACAAGAGCAA CGAGGGGAAA 
GAATTACTCG TCCCACTGAC GAGTTCTATG TATGTCCCTG GGAAGCTGCA TGATGTGGAA 
CACGTGCTCA TCGATGTGGG AACTGGGTAC TATGTAGAGA AGACAGCTGA GGATGCCAAG 
GACTTCTTCA AGAGGAAGAT AGATTTTCTA ACCAAGCAGA TGGAGAAAAT CCAACCAGCT 
CTTCAGGAGA AGCACGCCAT GAAACAGGCC GTCATGGAAA TGATGAGTCA GAAGATTCAG 
CAGCTCACAG CCCTGGGGGC AGCTCAGGCT ACTGCTAAGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002615.2
CDS	118..582
Translation	MAQSINITELNLPQLEMLKNQLDQEVEFLSTSIAQLKVVQTKYVEAKDCLNVLNKSNEGKELLVPLTSSMYVPGKLHDVEHVLIDVGTGYYVEKTAEDAKDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTALGAAQATAKA

Target ORF information:

RefSeq Version	NM_002624.3
Organism	Homo sapiens(human)
Definition	Homo sapiens prefoldin subunit 5 (PFDN5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002624.3

ORF Insert Sequence:

ATGGCGCAGT CTATTAACAT CACGGAGCTG AATCTGCCGC AGCTAGAAAT GCTCAAGAAC 
CAGCTGGACC AGGAAGTGGA GTTCTTGTCC ACGTCCATTG CTCAGCTCAA AGTGGTACAG 
ACCAAGTATG TGGAAGCCAA GGACTGTCTG AACGTGCTGA ACAAGAGCAA CGAGGGGAAA 
GAATTACTCG TCCCACTGAC GAGTTCTATG TATGTCCCTG GGAAGCTGCA TGATGTGGAA 
CACGTGCTCA TCGATGTGGG AACTGGGTAC TATGTAGAGA AGACAGCTGA GGATGCCAAG 
GACTTCTTCA AGAGGAAGAT AGATTTTCTA ACCAAGCAGA TGGAGAAAAT CCAACCAGCT 
CTTCAGGAGA AGCACGCCAT GAAACAGGCC GTCATGGAAA TGATGAGTCA GAAGATTCAG 
CAGCTCACAG CCCTGGGGGC AGCTCAGGCT ACTGCTAAGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFDN5 ( NM_002624.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002615.2 Homo sapiens prefoldin subunit 5 (PFDN5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19320
Clone ID Related Accession (Same CDS sequence)	NM_002624.3 , NM_002624.4
Accession Version	NM_002624.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	prefoldin subunit 5 isoform alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB055803.1 and AB055805.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_002624.3. Transcript Variant: This variant (1) encodes the longest isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB055803.1, SRR1163655.418136.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000334478.9/ ENSP00000334188.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCAGT CTATTAACAT CACGGAGCTG AATCTGCCGC AGCTAGAAAT GCTCAAGAAC 
CAGCTGGACC AGGAAGTGGA GTTCTTGTCC ACGTCCATTG CTCAGCTCAA AGTGGTACAG 
ACCAAGTATG TGGAAGCCAA GGACTGTCTG AACGTGCTGA ACAAGAGCAA CGAGGGGAAA 
GAATTACTCG TCCCACTGAC GAGTTCTATG TATGTCCCTG GGAAGCTGCA TGATGTGGAA 
CACGTGCTCA TCGATGTGGG AACTGGGTAC TATGTAGAGA AGACAGCTGA GGATGCCAAG 
GACTTCTTCA AGAGGAAGAT AGATTTTCTA ACCAAGCAGA TGGAGAAAAT CCAACCAGCT 
CTTCAGGAGA AGCACGCCAT GAAACAGGCC GTCATGGAAA TGATGAGTCA GAAGATTCAG 
CAGCTCACAG CCCTGGGGGC AGCTCAGGCT ACTGCTAAGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002615.2
CDS	27..491
Translation	MAQSINITELNLPQLEMLKNQLDQEVEFLSTSIAQLKVVQTKYVEAKDCLNVLNKSNEGKELLVPLTSSMYVPGKLHDVEHVLIDVGTGYYVEKTAEDAKDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTALGAAQATAKA

Target ORF information:

RefSeq Version	NM_002624.4
Organism	Homo sapiens(human)
Definition	Homo sapiens prefoldin subunit 5 (PFDN5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002624.4

ORF Insert Sequence:

ATGGCGCAGT CTATTAACAT CACGGAGCTG AATCTGCCGC AGCTAGAAAT GCTCAAGAAC 
CAGCTGGACC AGGAAGTGGA GTTCTTGTCC ACGTCCATTG CTCAGCTCAA AGTGGTACAG 
ACCAAGTATG TGGAAGCCAA GGACTGTCTG AACGTGCTGA ACAAGAGCAA CGAGGGGAAA 
GAATTACTCG TCCCACTGAC GAGTTCTATG TATGTCCCTG GGAAGCTGCA TGATGTGGAA 
CACGTGCTCA TCGATGTGGG AACTGGGTAC TATGTAGAGA AGACAGCTGA GGATGCCAAG 
GACTTCTTCA AGAGGAAGAT AGATTTTCTA ACCAAGCAGA TGGAGAAAAT CCAACCAGCT 
CTTCAGGAGA AGCACGCCAT GAAACAGGCC GTCATGGAAA TGATGAGTCA GAAGATTCAG 
CAGCTCACAG CCCTGGGGGC AGCTCAGGCT ACTGCTAAGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFDN5 ( NM_145897.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_665904.1 Homo sapiens prefoldin subunit 5 (PFDN5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu30182
Clone ID Related Accession (Same CDS sequence)	NM_145897.2
Accession Version	NM_145897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	prefoldin subunit 5 isoform gamma
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE392248.1 and AB055805.1. On Feb 28, 2006 this sequence version replaced NM_145897.1. Transcript Variant: This variant (3) lacks two exons in the coding region but maintains the reading frame, compared to variant 1. The encoded protein (isoform gamma) is shorter than isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB055805.1, SRR1163655.685509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCAGT CTATTAACAT CACGGAGCTG AATCTGCCGC AGCTAGAAAT GCTCAAGAAC 
CAGCTGGACC AGATGTATGT CCCTGGGAAG CTGCATGATG TGGAACACGT GCTCATCGAT 
GTGGGAACTG GGTACTATGT AGAGAAGACA GCTGAGGATG CCAAGGACTT CTTCAAGAGG 
AAGATAGATT TTCTAACCAA GCAGATGGAG AAAATCCAAC CAGCTCTTCA GGAGAAGCAC 
GCCATGAAAC AGGCCGTCAT GGAAATGATG AGTCAGAAGA TTCAGCAGCT CACAGCCCTG 
GGGGCAGCTC AGGCTACTGC TAAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665904.1
CDS	118..447
Translation	MAQSINITELNLPQLEMLKNQLDQMYVPGKLHDVEHVLIDVGTGYYVEKTAEDAKDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTALGAAQATAKA

Target ORF information:

RefSeq Version	NM_145897.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prefoldin subunit 5 (PFDN5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145897.2

ORF Insert Sequence:

ATGGCGCAGT CTATTAACAT CACGGAGCTG AATCTGCCGC AGCTAGAAAT GCTCAAGAAC 
CAGCTGGACC AGATGTATGT CCCTGGGAAG CTGCATGATG TGGAACACGT GCTCATCGAT 
GTGGGAACTG GGTACTATGT AGAGAAGACA GCTGAGGATG CCAAGGACTT CTTCAAGAGG 
AAGATAGATT TTCTAACCAA GCAGATGGAG AAAATCCAAC CAGCTCTTCA GGAGAAGCAC 
GCCATGAAAC AGGCCGTCAT GGAAATGATG AGTCAGAAGA TTCAGCAGCT CACAGCCCTG 
GGGGCAGCTC AGGCTACTGC TAAGGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.
PLoS genetics11(8)e1005428(2015 Aug)
Hasegawa Y,Taylor D,Ovchinnikov DA,Wolvetang EJ,de Torrent? L,Mar JC

A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways.
Cell158(2)434-448(2014 Jul)
Taipale M,Tucker G,Peng J,Krykbaeva I,Lin ZY,Larsen B,Choi H,Berger B,Gingras AC,Lindquist S

Prefoldin protects neuronal cells from polyglutamine toxicity by preventing aggregation formation.
The Journal of biological chemistry288(27)19958-72(2013 Jul)
Tashiro E,Zako T,Muto H,Itoo Y,S?rgjerd K,Terada N,Abe A,Miyazawa M,Kitamura A,Kitaura H,Kubota H,Maeda M,Momoi T,Iguchi-Ariga SM,Kinjo M,Ariga H

Rabring7 degrades c-Myc through complex formation with MM-1.
PloS one7(7)e41891(2012)
Narita R,Kitaura H,Torii A,Tashiro E,Miyazawa M,Ariga H,Iguchi-Ariga SM

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PFDN5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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