MAP3K20 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K20
Entrez Gene ID	51776
Full Name	mitogen-activated protein kinase kinase kinase 20
Synonyms	AZK,CNM6,MLK7,MLT,MLTK,MLTKalpha,MLTKbeta,MRK,SFMMP,ZAK,mlklak,pk
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 20 Names mitogen-activated protein kinase kinase kinase 20 mitogen-activated protein kinase kinase kinase MLT HCCS-4 MLK-like mitogen-activated protein triple kinase ZAK1 homolog, leucine zipper and sterile-alpha motif kinase human cervical cancer suppressor gene 4 protein mixed lineage kinase 7 mixed lineage kinase with a leucine zipper and a sterile alpha motif mixed lineage kinase-related kinase MRK-beta sterile alpha motif and leucine zipper containing kinase AZK
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q31.1
Summary	This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	609479
Disorder Html:	Split-foot malformation with mesoaxial polydactyly, 616890 (3); Centronuclear myopathy 6 with fiber-type disproportion, 617760 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017004323.1	XP_016859812.1	mitogen-activated protein kinase kinase kinase 20 isoform X2
NM_133646.3	NP_598407.1	mitogen-activated protein kinase kinase kinase 20 isoform 2
NM_133646.2	NP_598407.1	mitogen-activated protein kinase kinase kinase 20 isoform 2
NM_016653.2	NP_057737.2	mitogen-activated protein kinase kinase kinase 20 isoform 1
XM_005246640.2	XP_005246697.1	mitogen-activated protein kinase kinase kinase 20 isoform X1
XM_017004324.1	XP_016859813.1	mitogen-activated protein kinase kinase kinase 20 isoform X2
NM_016653.3	NP_057737.2	mitogen-activated protein kinase kinase kinase 20 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Zak	NP_075544.1
Rattus norvegicus (Norway rat)	Zak	XP_006234431.1
Xenopus tropicalis (tropical clawed frog)	LOC100495797	XP_002934652.1
Homo sapiens (human)	ZAK	NP_057737.2
Danio rerio (zebrafish)	si:dkey-15i8.3	XP_687660.2
Pan troglodytes (chimpanzee)	LOC459745	XP_003309476.1
Bos taurus (cattle)	ZAK	NP_001192422.1
Gallus gallus (chicken)	ZAK	XP_421996.4
	ZAK	XP_001086798.2
Canis lupus familiaris (dog)	ZAK	XP_535966.2

Gene Ontology
Bibliography

Related articles in PubMed

Novel genetic variants in the P38MAPK pathway gene ZAK and susceptibility to lung cancer.
Feng Y, Wang Y, Liu H, Liu Z, Mills C, Owzar K, Xie J, Han Y, Qian DC, Hung Rj RJ, Brhane Y, McLaughlin J, Brennan P, Bickeb?ller H, Rosenberger A, Houlston RS, Caporaso N, Landi MT, Br?ske I, Risch A, Ye Y, Wu X, Christiani DC, Amos CI, Wei Q
Molecular carcinogenesis57(2)216-224(2018 Feb)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Recessive mutations in the kinase ZAK - novel cause of congenital myopathy.
Title: Recessive mutations in the kinase ZAK cause a congenital myopathy with fibre type disproportion.

Gene set enrichment analysis disclosed a significant correlation between ZAK+ colorectal premalignant lesions and gene sets belonging to the MAPK/ERK and motility-related signaling pathways of the reactome database, strongly suggesting that ZAK induces such pro-tumoral reaction cascades in human cancers.
Title: The MAP3K ZAK, a novel modulator of ERK-dependent migration, is upregulated in colorectal cancer.

ZAK is a key player in mammalian limb patterning in humans and mice.
Title: Exome sequencing and CRISPR/Cas genome editing identify mutations of ZAK as a cause of limb defects in humans and mice.

ZAK kinase isoform TV1 is preferentially upregulated in gastric tumours and cell lines relative to normal samples. This pattern is also observed in colorectal, bladder and breast cancers.
Title: Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.

The long non-coding RNA URHC promotes cell proliferation and inhibits apoptosis by repressing ZAK expression through inactivation of the ERK/MAPK pathway.
Title: Long non-coding RNA URHC regulates cell proliferation and apoptosis via ZAK through the ERK/MAPK signaling pathway in hepatocellular carcinoma.

The following MAP3K20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13188	NM_133646.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu13188	XM_017004324.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu13188	XM_017004323.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19401	NM_016653.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu19401	XM_005246640.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu13188	NM_133646.2	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19401	NM_016653.2	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K20 ( NM_133646.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_598407.1 Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13188
Clone ID Related Accession (Same CDS sequence)	XM_017004323.1 , NM_133646.3 , NM_133646.2 , XM_017004324.1
Accession Version	NM_133646.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC019046.4 and AC013461.9. On May 31, 2019 this sequence version replaced NM_133646.2. Transcript Variant: This variant (2) lacks several exons in the coding region and contains a different 3' exon compared to variant 1. The resulting protein (isoform 2, also referred to as MRK-beta, MLTK-beta and MLK7) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF480462.1, SRR1803611.50106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACAA GTAACGGGGA GGGCCATGGC ATGAACCCAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGATGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAAGTCAACA TGGCTCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGATGAT 
GATGATGACG GTGAGGAGGA GGATAATGAC ATGGATAATA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598407.1
CDS	192..1559
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLAARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNPSLQAMMLMGFGDIFSMNKAGAVMHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDGEEEDNDMDNSE

Target ORF information:

RefSeq Version	NM_133646.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133646.3

ORF Insert Sequence:

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACAA GTAACGGGGA GGGCCATGGC ATGAACCCAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGATGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAAGTCAACA TGGCTCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGATGAT 
GATGATGACG GTGAGGAGGA GGATAATGAC ATGGATAATA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( NM_133646.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_598407.1 Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13188
Clone ID Related Accession (Same CDS sequence)	XM_017004323.1 , NM_133646.3 , NM_133646.2 , XM_017004324.1
Accession Version	NM_133646.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK056310.1, AF480462.1 and AL833180.1. On Dec 1, 2005 this sequence version replaced NM_133646.1. Transcript Variant: This variant (2) lacks several exons in the coding region and contains a different 3' exon compared to variant 1. The resulting protein (isoform 2, also referred to as MRK-beta, MLTK-beta and MLK7) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF480462.1, SRR1803613.22236.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACAA GTAACGGGGA GGGCCATGGC ATGAACCCAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGATGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAAGTCAACA TGGCTCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGATGAT 
GATGATGACG GTGAGGAGGA GGATAATGAC ATGGATAATA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598407.1
CDS	201..1568
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLAARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNPSLQAMMLMGFGDIFSMNKAGAVMHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDGEEEDNDMDNSE

Target ORF information:

RefSeq Version	NM_133646.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133646.2

ORF Insert Sequence:

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACAA GTAACGGGGA GGGCCATGGC ATGAACCCAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGATGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAAGTCAACA TGGCTCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGATGAT 
GATGATGACG GTGAGGAGGA GGATAATGAC ATGGATAATA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( XM_017004323.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859812.1 Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu13188
Clone ID Related Accession (Same CDS sequence)	XM_017004323.1 , NM_133646.3 , NM_133646.2 , XM_017004324.1
Accession Version	XM_017004323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACAA GTAACGGGGA GGGCCATGGC ATGAACCCAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGATGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAAGTCAACA TGGCTCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGATGAT 
GATGATGACG GTGAGGAGGA GGATAATGAC ATGGATAATA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859812.1
CDS	247..1614
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLAARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNPSLQAMMLMGFGDIFSMNKAGAVMHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDGEEEDNDMDNSE

Target ORF information:

RefSeq Version	XM_017004323.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004323.1

ORF Insert Sequence:

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACAA GTAACGGGGA GGGCCATGGC ATGAACCCAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGATGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAAGTCAACA TGGCTCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGATGAT 
GATGATGACG GTGAGGAGGA GGATAATGAC ATGGATAATA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( XM_017004324.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859813.1 Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X3, mRNA.

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CloneID	OHu13188
Clone ID Related Accession (Same CDS sequence)	XM_017004323.1 , NM_133646.3 , NM_133646.2 , XM_017004324.1
Accession Version	XM_017004324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACAA GTAACGGGGA GGGCCATGGC ATGAACCCAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGATGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAAGTCAACA TGGCTCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGATGAT 
GATGATGACG GTGAGGAGGA GGATAATGAC ATGGATAATA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859813.1
CDS	271..1638
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLAARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNPSLQAMMLMGFGDIFSMNKAGAVMHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDGEEEDNDMDNSE

Target ORF information:

RefSeq Version	XM_017004324.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004324.1

ORF Insert Sequence:

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTT CTCTTGCCTC TTGCTGCAAG AATGTCTGAG 
GAGTCTTACT TTGAATCTAA AACAGAGGAG TCAAACAGTG CAGAGATGTC ATGTCAGATC 
ACAGCAACAA GTAACGGGGA GGGCCATGGC ATGAACCCAA GTCTGCAGGC CATGATGCTG 
ATGGGCTTTG GGGATATCTT CTCAATGAAC AAAGCAGGAG CTGTGATGCA TTCTGGGATG 
CAGATAAACA TGCAAGCCAA GCAGAATTCT TCCAAAACCA CATCTAAGAG AAGGGGGAAG 
AAAGTCAACA TGGCTCTGGG GTTCAGTGAT TTTGACTTGT CAGAAGGTGA CGATGATGAT 
GATGATGACG GTGAGGAGGA GGATAATGAC ATGGATAATA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( NM_016653.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057737.2 Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 1, mRNA.

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CloneID	OHu19401
Clone ID Related Accession (Same CDS sequence)	NM_016653.2 , XM_005246640.2 , NM_016653.3
Accession Version	NM_016653.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-10
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA993056.1, AB030034.1, AF251441.1, CB955745.1, AF480461.1, AI799498.1, BF055268.1 and AA258354.1. This sequence is a reference standard in the RefSeqGene project. On Dec 1, 2005 this sequence version replaced NM_016653.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1, also referred to as MRK-alpha and MLTK-alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF480461.1, AF251441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCATGGACG 
GAAGACGATG TGTATTGTTG GGTTCAGCAG CTCGTCAGAA AAGGTGACTC TTCAGCAGAG 
ATGAGTGTAT ATGCAAGCTT GTTTAAAGAA AACAACATTA CAGGGAAGCG GCTGCTGCTG 
CTGGAGGAAG AAGACCTGAA AGACATGGGC ATTGTCTCCA AGGGGCATAT CATTCACTTC 
AAGTCAGCCA TTGAGAAATT AACCCATGAT TACATAAATT TGTTTCACTT CCCACCACTA 
ATTAAGGACT CAGGAGGTGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTGGAACTG 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACCTACATAA AAGATGTGAC ATTCAACACT 
AACCTACCTG ATGCGGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATTGTAGGAA TAGCAAAAAG TCAGACTGTG GAGTGCACTG TCACATATGA GAGTGATGTT 
AGAACTCCAA AAAGCACTAA ACATGTCCAT TCGATTCAGT GGAGTAGAAC AAAACCTCAG 
GATGAAGTGA AAGCAGTCCA ACTTGCCATT CAGACATTAT TCACCAATTC AGATGGCAAC 
CCTGGAAGCA GGTCCGACTC AAGTGCTGAT TGCCAGTGGT TAGATACTCT GAGGATGCGG 
CAGATTGCAT CCAACACTTC TTTACAGCGT TCCCAGAGCA ATCCTATTCT GGGGTCACCG 
TTCTTCTCAC ACTTTGATGG CCAGGATTCC TACGCTGCTG CTGTGAGACG GCCCCAGGTG 
CCCATTAAGT ATCAACAGAT TACACCTGTG AACCAGTCCA GAAGCTCGTC TCCTACTCAG 
TATGGACTGA CCAAAAACTT CTCTTCCCTA CATCTCAACT CTAGGGACAG TGGCTTTTCC 
AGTGGCAATA CTGACACCTC TTCAGAGAGG GGTCGATACT CAGACAGAAG CAGGAACAAA 
TATGGACGTG GTAGTATATC ACTCAATTCT TCTCCTAGAG GAAGATACAG TGGAAAGAGT 
CAGCATTCCA CTCCTTCAAG AGGAAGATAC CCTGGAAAGT TCTACAGGGT TTCTCAGTCA 
GCACTCAATC CTCACCAGTC GCCTGACTTC AAGAGAAGCC CCAGGGACCT CCACCAACCC 
AACACCATAC CAGGGATGCC TTTGCACCCT GAGACTGACT CAAGAGCCAG TGAAGAGGAC 
AGCAAAGTCA GCGAAGGGGG CTGGACAAAA GTGGAATACC GGAAAAAGCC CCACAGGCCA 
TCTCCCGCCA AAACCAATAA AGAGAGAGCC AGAGGGGACC ACCGTGGATG GAGAAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057737.2
CDS	201..2603
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYCWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYINLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIVGIAKSQTVECTVTYESDVRTPKSTKHVHSIQWSRTKPQDEVKAVQLAIQTLFTNSDGNPGSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSPFFSHFDGQDSYAAAVRRPQVPIKYQQITPVNQSRSSSPTQYGLTKNFSSLHLNSRDSGFSSGNTDTSSERGRYSDRSRNKYGRGSISLNSSPRGRYSGKSQHSTPSRGRYPGKFYRVSQSALNPHQSPDFKRSPRDLHQPNTIPGMPLHPETDSRASEEDSKVSEGGWTKVEYRKKPHRPSPAKTNKERARGDHRGWRNF

Target ORF information:

RefSeq Version	NM_016653.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016653.2

ORF Insert Sequence:

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCATGGACG 
GAAGACGATG TGTATTGTTG GGTTCAGCAG CTCGTCAGAA AAGGTGACTC TTCAGCAGAG 
ATGAGTGTAT ATGCAAGCTT GTTTAAAGAA AACAACATTA CAGGGAAGCG GCTGCTGCTG 
CTGGAGGAAG AAGACCTGAA AGACATGGGC ATTGTCTCCA AGGGGCATAT CATTCACTTC 
AAGTCAGCCA TTGAGAAATT AACCCATGAT TACATAAATT TGTTTCACTT CCCACCACTA 
ATTAAGGACT CAGGAGGTGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTGGAACTG 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACCTACATAA AAGATGTGAC ATTCAACACT 
AACCTACCTG ATGCGGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATTGTAGGAA TAGCAAAAAG TCAGACTGTG GAGTGCACTG TCACATATGA GAGTGATGTT 
AGAACTCCAA AAAGCACTAA ACATGTCCAT TCGATTCAGT GGAGTAGAAC AAAACCTCAG 
GATGAAGTGA AAGCAGTCCA ACTTGCCATT CAGACATTAT TCACCAATTC AGATGGCAAC 
CCTGGAAGCA GGTCCGACTC AAGTGCTGAT TGCCAGTGGT TAGATACTCT GAGGATGCGG 
CAGATTGCAT CCAACACTTC TTTACAGCGT TCCCAGAGCA ATCCTATTCT GGGGTCACCG 
TTCTTCTCAC ACTTTGATGG CCAGGATTCC TACGCTGCTG CTGTGAGACG GCCCCAGGTG 
CCCATTAAGT ATCAACAGAT TACACCTGTG AACCAGTCCA GAAGCTCGTC TCCTACTCAG 
TATGGACTGA CCAAAAACTT CTCTTCCCTA CATCTCAACT CTAGGGACAG TGGCTTTTCC 
AGTGGCAATA CTGACACCTC TTCAGAGAGG GGTCGATACT CAGACAGAAG CAGGAACAAA 
TATGGACGTG GTAGTATATC ACTCAATTCT TCTCCTAGAG GAAGATACAG TGGAAAGAGT 
CAGCATTCCA CTCCTTCAAG AGGAAGATAC CCTGGAAAGT TCTACAGGGT TTCTCAGTCA 
GCACTCAATC CTCACCAGTC GCCTGACTTC AAGAGAAGCC CCAGGGACCT CCACCAACCC 
AACACCATAC CAGGGATGCC TTTGCACCCT GAGACTGACT CAAGAGCCAG TGAAGAGGAC 
AGCAAAGTCA GCGAAGGGGG CTGGACAAAA GTGGAATACC GGAAAAAGCC CCACAGGCCA 
TCTCCCGCCA AAACCAATAA AGAGAGAGCC AGAGGGGACC ACCGTGGATG GAGAAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( NM_016653.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057737.2 Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19401
Clone ID Related Accession (Same CDS sequence)	NM_016653.2 , XM_005246640.2 , NM_016653.3
Accession Version	NM_016653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC019046.4, AC013461.9 and AC092573.2. On Nov 23, 2018 this sequence version replaced NM_016653.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1, also referred to as MRK-alpha and MLTK-alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF480461.1, AF251441.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000375213.8/ ENSP00000364361.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCATGGACG 
GAAGACGATG TGTATTGTTG GGTTCAGCAG CTCGTCAGAA AAGGTGACTC TTCAGCAGAG 
ATGAGTGTAT ATGCAAGCTT GTTTAAAGAA AACAACATTA CAGGGAAGCG GCTGCTGCTG 
CTGGAGGAAG AAGACCTGAA AGACATGGGC ATTGTCTCCA AGGGGCATAT CATTCACTTC 
AAGTCAGCCA TTGAGAAATT AACCCATGAT TACATAAATT TGTTTCACTT CCCACCACTA 
ATTAAGGACT CAGGAGGTGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTGGAACTG 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACCTACATAA AAGATGTGAC ATTCAACACT 
AACCTACCTG ATGCGGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATTGTAGGAA TAGCAAAAAG TCAGACTGTG GAGTGCACTG TCACATATGA GAGTGATGTT 
AGAACTCCAA AAAGCACTAA ACATGTCCAT TCGATTCAGT GGAGTAGAAC AAAACCTCAG 
GATGAAGTGA AAGCAGTCCA ACTTGCCATT CAGACATTAT TCACCAATTC AGATGGCAAC 
CCTGGAAGCA GGTCCGACTC AAGTGCTGAT TGCCAGTGGT TAGATACTCT GAGGATGCGG 
CAGATTGCAT CCAACACTTC TTTACAGCGT TCCCAGAGCA ATCCTATTCT GGGGTCACCG 
TTCTTCTCAC ACTTTGATGG CCAGGATTCC TACGCTGCTG CTGTGAGACG GCCCCAGGTG 
CCCATTAAGT ATCAACAGAT TACACCTGTG AACCAGTCCA GAAGCTCGTC TCCTACTCAG 
TATGGACTGA CCAAAAACTT CTCTTCCCTA CATCTCAACT CTAGGGACAG TGGCTTTTCC 
AGTGGCAATA CTGACACCTC TTCAGAGAGG GGTCGATACT CAGACAGAAG CAGGAACAAA 
TATGGACGTG GTAGTATATC ACTCAATTCT TCTCCTAGAG GAAGATACAG TGGAAAGAGT 
CAGCATTCCA CTCCTTCAAG AGGAAGATAC CCTGGAAAGT TCTACAGGGT TTCTCAGTCA 
GCACTCAATC CTCACCAGTC GCCTGACTTC AAGAGAAGCC CCAGGGACCT CCACCAACCC 
AACACCATAC CAGGGATGCC TTTGCACCCT GAGACTGACT CAAGAGCCAG TGAAGAGGAC 
AGCAAAGTCA GCGAAGGGGG CTGGACAAAA GTGGAATACC GGAAAAAGCC CCACAGGCCA 
TCTCCCGCCA AAACCAATAA AGAGAGAGCC AGAGGGGACC ACCGTGGATG GAGAAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057737.2
CDS	192..2594
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYCWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYINLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIVGIAKSQTVECTVTYESDVRTPKSTKHVHSIQWSRTKPQDEVKAVQLAIQTLFTNSDGNPGSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSPFFSHFDGQDSYAAAVRRPQVPIKYQQITPVNQSRSSSPTQYGLTKNFSSLHLNSRDSGFSSGNTDTSSERGRYSDRSRNKYGRGSISLNSSPRGRYSGKSQHSTPSRGRYPGKFYRVSQSALNPHQSPDFKRSPRDLHQPNTIPGMPLHPETDSRASEEDSKVSEGGWTKVEYRKKPHRPSPAKTNKERARGDHRGWRNF

Target ORF information:

RefSeq Version	NM_016653.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016653.3

ORF Insert Sequence:

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCATGGACG 
GAAGACGATG TGTATTGTTG GGTTCAGCAG CTCGTCAGAA AAGGTGACTC TTCAGCAGAG 
ATGAGTGTAT ATGCAAGCTT GTTTAAAGAA AACAACATTA CAGGGAAGCG GCTGCTGCTG 
CTGGAGGAAG AAGACCTGAA AGACATGGGC ATTGTCTCCA AGGGGCATAT CATTCACTTC 
AAGTCAGCCA TTGAGAAATT AACCCATGAT TACATAAATT TGTTTCACTT CCCACCACTA 
ATTAAGGACT CAGGAGGTGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTGGAACTG 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACCTACATAA AAGATGTGAC ATTCAACACT 
AACCTACCTG ATGCGGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATTGTAGGAA TAGCAAAAAG TCAGACTGTG GAGTGCACTG TCACATATGA GAGTGATGTT 
AGAACTCCAA AAAGCACTAA ACATGTCCAT TCGATTCAGT GGAGTAGAAC AAAACCTCAG 
GATGAAGTGA AAGCAGTCCA ACTTGCCATT CAGACATTAT TCACCAATTC AGATGGCAAC 
CCTGGAAGCA GGTCCGACTC AAGTGCTGAT TGCCAGTGGT TAGATACTCT GAGGATGCGG 
CAGATTGCAT CCAACACTTC TTTACAGCGT TCCCAGAGCA ATCCTATTCT GGGGTCACCG 
TTCTTCTCAC ACTTTGATGG CCAGGATTCC TACGCTGCTG CTGTGAGACG GCCCCAGGTG 
CCCATTAAGT ATCAACAGAT TACACCTGTG AACCAGTCCA GAAGCTCGTC TCCTACTCAG 
TATGGACTGA CCAAAAACTT CTCTTCCCTA CATCTCAACT CTAGGGACAG TGGCTTTTCC 
AGTGGCAATA CTGACACCTC TTCAGAGAGG GGTCGATACT CAGACAGAAG CAGGAACAAA 
TATGGACGTG GTAGTATATC ACTCAATTCT TCTCCTAGAG GAAGATACAG TGGAAAGAGT 
CAGCATTCCA CTCCTTCAAG AGGAAGATAC CCTGGAAAGT TCTACAGGGT TTCTCAGTCA 
GCACTCAATC CTCACCAGTC GCCTGACTTC AAGAGAAGCC CCAGGGACCT CCACCAACCC 
AACACCATAC CAGGGATGCC TTTGCACCCT GAGACTGACT CAAGAGCCAG TGAAGAGGAC 
AGCAAAGTCA GCGAAGGGGG CTGGACAAAA GTGGAATACC GGAAAAAGCC CCACAGGCCA 
TCTCCCGCCA AAACCAATAA AGAGAGAGCC AGAGGGGACC ACCGTGGATG GAGAAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K20 ( XM_005246640.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005246697.1 Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X1, mRNA.

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CloneID	OHu19401
Clone ID Related Accession (Same CDS sequence)	NM_016653.2 , XM_005246640.2 , NM_016653.3
Accession Version	XM_005246640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCATGGACG 
GAAGACGATG TGTATTGTTG GGTTCAGCAG CTCGTCAGAA AAGGTGACTC TTCAGCAGAG 
ATGAGTGTAT ATGCAAGCTT GTTTAAAGAA AACAACATTA CAGGGAAGCG GCTGCTGCTG 
CTGGAGGAAG AAGACCTGAA AGACATGGGC ATTGTCTCCA AGGGGCATAT CATTCACTTC 
AAGTCAGCCA TTGAGAAATT AACCCATGAT TACATAAATT TGTTTCACTT CCCACCACTA 
ATTAAGGACT CAGGAGGTGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTGGAACTG 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACCTACATAA AAGATGTGAC ATTCAACACT 
AACCTACCTG ATGCGGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATTGTAGGAA TAGCAAAAAG TCAGACTGTG GAGTGCACTG TCACATATGA GAGTGATGTT 
AGAACTCCAA AAAGCACTAA ACATGTCCAT TCGATTCAGT GGAGTAGAAC AAAACCTCAG 
GATGAAGTGA AAGCAGTCCA ACTTGCCATT CAGACATTAT TCACCAATTC AGATGGCAAC 
CCTGGAAGCA GGTCCGACTC AAGTGCTGAT TGCCAGTGGT TAGATACTCT GAGGATGCGG 
CAGATTGCAT CCAACACTTC TTTACAGCGT TCCCAGAGCA ATCCTATTCT GGGGTCACCG 
TTCTTCTCAC ACTTTGATGG CCAGGATTCC TACGCTGCTG CTGTGAGACG GCCCCAGGTG 
CCCATTAAGT ATCAACAGAT TACACCTGTG AACCAGTCCA GAAGCTCGTC TCCTACTCAG 
TATGGACTGA CCAAAAACTT CTCTTCCCTA CATCTCAACT CTAGGGACAG TGGCTTTTCC 
AGTGGCAATA CTGACACCTC TTCAGAGAGG GGTCGATACT CAGACAGAAG CAGGAACAAA 
TATGGACGTG GTAGTATATC ACTCAATTCT TCTCCTAGAG GAAGATACAG TGGAAAGAGT 
CAGCATTCCA CTCCTTCAAG AGGAAGATAC CCTGGAAAGT TCTACAGGGT TTCTCAGTCA 
GCACTCAATC CTCACCAGTC GCCTGACTTC AAGAGAAGCC CCAGGGACCT CCACCAACCC 
AACACCATAC CAGGGATGCC TTTGCACCCT GAGACTGACT CAAGAGCCAG TGAAGAGGAC 
AGCAAAGTCA GCGAAGGGGG CTGGACAAAA GTGGAATACC GGAAAAAGCC CCACAGGCCA 
TCTCCCGCCA AAACCAATAA AGAGAGAGCC AGAGGGGACC ACCGTGGATG GAGAAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005246697.1
CDS	247..2649
Translation	MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYCWVQQLVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYINLFHFPPLIKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKMTKPPFVMEKWIVGIAKSQTVECTVTYESDVRTPKSTKHVHSIQWSRTKPQDEVKAVQLAIQTLFTNSDGNPGSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSPFFSHFDGQDSYAAAVRRPQVPIKYQQITPVNQSRSSSPTQYGLTKNFSSLHLNSRDSGFSSGNTDTSSERGRYSDRSRNKYGRGSISLNSSPRGRYSGKSQHSTPSRGRYPGKFYRVSQSALNPHQSPDFKRSPRDLHQPNTIPGMPLHPETDSRASEEDSKVSEGGWTKVEYRKKPHRPSPAKTNKERARGDHRGWRNF

Target ORF information:

RefSeq Version	XM_005246640.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 20 (MAP3K20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246640.2

ORF Insert Sequence:

ATGTCGTCTC TCGGTGCCTC CTTTGTGCAA ATTAAATTTG ATGACTTGCA GTTTTTTGAA 
AACTGCGGTG GAGGAAGTTT TGGGAGTGTT TATCGAGCCA AATGGATATC ACAGGACAAG 
GAGGTGGCTG TAAAGAAGCT CCTCAAAATA GAGAAAGAGG CAGAAATACT CAGTGTCCTC 
AGTCACAGAA ACATCATCCA GTTTTATGGA GTAATTCTTG AACCTCCCAA CTATGGCATT 
GTCACAGAAT ATGCTTCTCT GGGATCACTC TATGATTACA TTAACAGTAA CAGAAGTGAG 
GAGATGGATA TGGATCACAT TATGACCTGG GCCACTGATG TAGCCAAAGG AATGCATTAT 
TTACATATGG AGGCTCCTGT CAAGGTGATT CACAGAGACC TCAAGTCAAG AAACGTTGTT 
ATAGCTGCTG ATGGAGTATT GAAGATCTGT GACTTTGGTG CCTCTCGGTT CCATAACCAT 
ACAACACACA TGTCCTTGGT TGGAACTTTC CCATGGATGG CTCCAGAAGT TATCCAGAGT 
CTCCCTGTGT CAGAAACTTG TGACACATAT TCCTATGGTG TGGTTCTCTG GGAGATGCTA 
ACAAGGGAGG TCCCCTTTAA AGGTTTGGAA GGATTACAAG TAGCTTGGCT TGTAGTGGAA 
AAAAACGAGA GATTAACCAT TCCAAGCAGT TGCCCCAGAA GTTTTGCTGA ACTGTTACAT 
CAGTGTTGGG AAGCTGATGC CAAGAAACGG CCATCATTCA AGCAAATCAT TTCAATCCTG 
GAGTCCATGT CAAATGACAC GAGCCTTCCT GACAAGTGTA ACTCATTCCT ACACAACAAG 
GCGGAGTGGA GGTGCGAAAT TGAGGCAACT CTTGAGAGGC TAAAGAAACT AGAGCGTGAT 
CTCAGCTTTA AGGAGCAGGA GCTTAAAGAA CGAGAAAGAC GTTTAAAGAT GTGGGAGCAA 
AAGCTGACAG AGCAGTCCAA CACCCCGCTG CTGCCTTCCT TTGAGATTGG TGCATGGACG 
GAAGACGATG TGTATTGTTG GGTTCAGCAG CTCGTCAGAA AAGGTGACTC TTCAGCAGAG 
ATGAGTGTAT ATGCAAGCTT GTTTAAAGAA AACAACATTA CAGGGAAGCG GCTGCTGCTG 
CTGGAGGAAG AAGACCTGAA AGACATGGGC ATTGTCTCCA AGGGGCATAT CATTCACTTC 
AAGTCAGCCA TTGAGAAATT AACCCATGAT TACATAAATT TGTTTCACTT CCCACCACTA 
ATTAAGGACT CAGGAGGTGA ACCTGAAGAA AATGAGGAAA AAATAGTGAA CCTGGAACTG 
GTTTTTGGTT TTCACTTGAA ACCAGGAACT GGCCCACAGG ATTGTAAGTG GAAAATGTAT 
ATGGAGATGG ATGGGGATGA AATTGCAATA ACCTACATAA AAGATGTGAC ATTCAACACT 
AACCTACCTG ATGCGGAGAT TTTAAAGATG ACAAAGCCAC CATTTGTAAT GGAGAAGTGG 
ATTGTAGGAA TAGCAAAAAG TCAGACTGTG GAGTGCACTG TCACATATGA GAGTGATGTT 
AGAACTCCAA AAAGCACTAA ACATGTCCAT TCGATTCAGT GGAGTAGAAC AAAACCTCAG 
GATGAAGTGA AAGCAGTCCA ACTTGCCATT CAGACATTAT TCACCAATTC AGATGGCAAC 
CCTGGAAGCA GGTCCGACTC AAGTGCTGAT TGCCAGTGGT TAGATACTCT GAGGATGCGG 
CAGATTGCAT CCAACACTTC TTTACAGCGT TCCCAGAGCA ATCCTATTCT GGGGTCACCG 
TTCTTCTCAC ACTTTGATGG CCAGGATTCC TACGCTGCTG CTGTGAGACG GCCCCAGGTG 
CCCATTAAGT ATCAACAGAT TACACCTGTG AACCAGTCCA GAAGCTCGTC TCCTACTCAG 
TATGGACTGA CCAAAAACTT CTCTTCCCTA CATCTCAACT CTAGGGACAG TGGCTTTTCC 
AGTGGCAATA CTGACACCTC TTCAGAGAGG GGTCGATACT CAGACAGAAG CAGGAACAAA 
TATGGACGTG GTAGTATATC ACTCAATTCT TCTCCTAGAG GAAGATACAG TGGAAAGAGT 
CAGCATTCCA CTCCTTCAAG AGGAAGATAC CCTGGAAAGT TCTACAGGGT TTCTCAGTCA 
GCACTCAATC CTCACCAGTC GCCTGACTTC AAGAGAAGCC CCAGGGACCT CCACCAACCC 
AACACCATAC CAGGGATGCC TTTGCACCCT GAGACTGACT CAAGAGCCAG TGAAGAGGAC 
AGCAAAGTCA GCGAAGGGGG CTGGACAAAA GTGGAATACC GGAAAAAGCC CCACAGGCCA 
TCTCCCGCCA AAACCAATAA AGAGAGAGCC AGAGGGGACC ACCGTGGATG GAGAAACTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel genetic variants in the P38MAPK pathway gene ZAK and susceptibility to lung cancer.
Molecular carcinogenesis57(2)216-224(2018 Feb)
Feng Y,Wang Y,Liu H,Liu Z,Mills C,Owzar K,Xie J,Han Y,Qian DC,Hung Rj RJ,Brhane Y,McLaughlin J,Brennan P,Bickeb?ller H,Rosenberger A,Houlston RS,Caporaso N,Landi MT,Br?ske I,Risch A,Ye Y,Wu X,Christiani DC,Amos CI,Wei Q

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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MAP3K20 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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