DBR1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DBR1
Entrez Gene ID	51163
Full Name	debranching RNA lariats 1
General protein information	Preferred Names debranching RNA lariats 1 Names lariat debranching enzyme RNA lariat debranching enzyme debranching enzyme homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q22.3
Summary	The protein encoded by this gene is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. [provided by RefSeq, Nov 2011].
Disorder MIM:	607024

mRNA and Protein(s)

mRNA	Protein	Name
NM_016216.4	NP_057300.2	lariat debranching enzyme
NM_016216.3	NP_057300.2	lariat debranching enzyme

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dbr1	NP_955947.1
Caenorhabditis elegans (roundworm)	dbr-1	NP_491868.2
Schizosaccharomyces pombe (fission yeast)	dbr1	NP_593470.2
Magnaporthe oryzae (rice blast fungus)	MGG_03897	XP_003719979.1
Neurospora crassa	NCU03409	XP_956856.1
Bos taurus (cattle)	DBR1	NP_001073759.1
Homo sapiens (human)	DBR1	NP_057300.2
	DBR1	XP_001114637.1
Rattus norvegicus (Norway rat)	Dbr1	NP_001102907.1
Gallus gallus (chicken)	DBR1	NP_001026737.1
Pan troglodytes (chimpanzee)	DBR1	XP_526319.2
Drosophila melanogaster (fruit fly)	ldbr	NP_996022.1
Oryza sativa (rice)	Os03g0725300	NP_001051129.2
Xenopus tropicalis (tropical clawed frog)	dbr1	NP_988947.1
Canis lupus familiaris (dog)	DBR1	XP_852077.1
Mus musculus (house mouse)	Dbr1	NP_113580.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006805	XP_308942.4
Arabidopsis thaliana (thale cress)	DBR1	NP_567881.1

Gene Ontology
Bibliography

Related articles in PubMed

Conformational Changes in the 5' End of the HIV-1 Genome Dependent on the Debranching Enzyme DBR1 during Early Stages of Infection.
Galvis AE, Fisher HE, Fan H, Camerini D
Journal of virology91(23)(2017 Dec)

Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development.
Han B, Park HK, Ching T, Panneerselvam J, Wang H, Shen Y, Zhang J, Li L, Che R, Garmire L, Fei P
Oncogene36(38)5382-5391(2017 Sep)

In silico functional dissection of saturation mutagenesis: Interpreting the relationship between phenotypes and changes in protein stability, interactions and activity.
Pires DE, Chen J, Blundell TL, Ascher DB
Scientific reports619848(2016 Jan)

Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.
Masaki S, Yoshimoto R, Kaida D, Hata A, Satoh T, Ohno M, Kataoka N
International journal of molecular sciences16(2)3705-21(2015 Feb)

Impairment of HIV-1 cDNA synthesis by DBR1 knockdown.
Galvis AE, Fisher HE, Nitta T, Fan H, Camerini D
Journal of virology88(12)7054-69(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings support a new view of the early steps in HIV genome replication - they support a model in which lariat formation of viral genomic RNA is important for minus-strand transfer, with DBR1 being required to resolve the lariat to allow reverse transcription to proceed
Title: Conformational Changes in the 5' End of the HIV-1 Genome Dependent on the Debranching Enzyme DBR1 during Early Stages of Infection.

Transcriptomic sequencing confirmed the effect of hDBR1 on RNA splicing, and metabolite profiling supported the observation that neoplasm is triggered by a decrease in hDBR1 expression both in vitro and in vivo.
Title: Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development.

saturation mutagenesis of DBR1 and Gal4 and show that the experimental phenotypes for over 80% of the mutations correlate well with predicted effects of mutations on protein stability and RNA binding affinity.
Title: In silico functional dissection of saturation mutagenesis: Interpreting the relationship between phenotypes and changes in protein stability, interactions and activity.

Results of protein-protein interaction between human Dbr1 and factors found in the Intron Large complex identify Xab2 and a novel protein CWF19L1 as specific interactors of DBR1.
Title: Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.

Inhibition of DBR1 caused a significant reduction in the formation of intermediate and full-length HIV-1 cDNA.
Title: Impairment of HIV-1 cDNA synthesis by DBR1 knockdown.

The following DBR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DBR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11162	NM_016216.4 Latest version!	Homo sapiens debranching RNA lariats 1 (DBR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu11162	NM_016216.3	Homo sapiens debranching RNA lariats 1 (DBR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DBR1 ( NM_016216.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057300.2 Homo sapiens debranching RNA lariats 1 (DBR1), mRNA.

Price & Availability↑

CloneID	OHu11162
Clone ID Related Accession (Same CDS sequence)	NM_016216.4 , NM_016216.3
Accession Version	NM_016216.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	lariat debranching enzyme
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL702928.1, BC009472.1, AC023049.14, CD677175.1 and BF987317.1. On May 17, 2019 this sequence version replaced NM_016216.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.257390.1, SRR7346977.766999.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260803.9/ ENSP00000260803.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGGTGG CTGTGGCTGG CTGCTGCCAC GGCGAGCTGG ATAAGATCTA TGAGACGCTG 
GCGCTGGCAG AGCGGCGCGG CCCGGGGCCT GTCGACCTCT TGCTGTGCTG CGGCGACTTC 
CAGGCGGTGC GCAACGAGGC GGATCTACGC TGCATGGCCG TGCCGCCCAA GTATCGTCAC 
ATGCAAACCT TCTACAGGTA TTACTCTGGA GAGAAAAAGG CTCCAGTTCT CACGCTCTTC 
ATTGGGGGAA ACCATGAAGC CTCAAATCAT TTGCAAGAGT TACCCTATGG TGGCTGGGTG 
GCACCAAACA TTTATTATTT AGGTTTGGCT GGTGTGGTAA AATACCGAGG TGTAAGGATC 
GGTGGAATCT CTGGTATCTT TAAATCTCAT GACTATCGAA AAGGTCATTT TGAGTGCCCC 
CCTTATAATT CATCTACAAT CAGGAGTATA TATCATGTGA GAAATATTGA AGTCTATAAA 
TTAAAACAGC TGAAGCAGCC TATAGATATA TTCTTGTCTC ATGATTGGCC AAGAAGTATA 
TATCATTATG GAAATAAGAA GCAACTTCTT AAGACTAAAT CTTTTTTCCG ACAAGAAGTG 
GAAAATAACA CATTAGGAAG TCCAGCTGCC TCAGAGCTTT TAGAGCATCT CAAACCTACT 
TATTGGTTTT CTGCCCACCT TCATGTGAAG TTTGCCGCCT TGATGCAGCA TCAGGCAAAG 
GATAAAGGAC AGACAGCCAG AGCAACCAAA TTTTTAGCCT TGGACAAATG CTTACCACAT 
AGAGATTTTC TTCAGATATT AGAGATAGAA CATGACCCCA GTGCTCCTGA TTACTTGGAA 
TATGATATTG AATGGCTCAC TATTCTCAGG GCTACGGATG ATCTTATTAA TGTGACTGGG 
CGCCTGTGGA ATATGCCAGA AAATAATGGC CTGCATGCAA GGTGGGATTA TAGTGCAACA 
GAAGAAGGTA TGAAAGAAGT ATTGGAAAAA TTGAATCATG ATCTCAAGGT TCCATGTAAC 
TTTAGTGTAA CAGCTGCTTG TTATGATCCT AGCAAGCCAC AGACACAAAT GCAGCTGATT 
CATAGGATCA ATCCTCAGAC AACTGAATTT TGTGCCCAAC TTGGCATCAT AGACATCAAT 
GTTAGGCTTC AGAAGTCCAA GGAAGAACAT CATGTGTGTG GTGAATATGA AGAACAGGAT 
GATGTGGAGA GTAATGACTC TGGAGAAGAC CAGAGTGAAT ATAATACAGA CACATCTGCT 
CTGTCTTCTA TTAATCCAGA TGAAATAATG TTAGATGAAG AAGAAGATGA AGATAGTATT 
GTAAGTGCAC ATAGTGGCAT GAATACACCA TCGGTAGAAC CTTCTGATCA AGCTTCTGAG 
TTTTCTGCAA GTTTCTCTGA TGTCAGGATC TTGCCAGGCT CTATGATTGT ATCTTCTGAT 
GATACGGTGG ATTCCACAAT TGATAGAGAG GGGAAACCTG GTGGGACTGT GGAGTCAGGG 
AATGGAGAGG ACTTAACCAA GGTGCCATTG AAGAGGCTGA GTGATGAACA TGAACCTGAA 
CAAAGAAAGA AAATTAAGAG GAGGAATCAA GCCATTTACG CTGCAGTGGA TGATGATGAT 
GACGATGCAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057300.2
CDS	127..1761
Translation	MRVAVAGCCHGELDKIYETLALAERRGPGPVDLLLCCGDFQAVRNEADLRCMAVPPKYRHMQTFYRYYSGEKKAPVLTLFIGGNHEASNHLQELPYGGWVAPNIYYLGLAGVVKYRGVRIGGISGIFKSHDYRKGHFECPPYNSSTIRSIYHVRNIEVYKLKQLKQPIDIFLSHDWPRSIYHYGNKKQLLKTKSFFRQEVENNTLGSPAASELLEHLKPTYWFSAHLHVKFAALMQHQAKDKGQTARATKFLALDKCLPHRDFLQILEIEHDPSAPDYLEYDIEWLTILRATDDLINVTGRLWNMPENNGLHARWDYSATEEGMKEVLEKLNHDLKVPCNFSVTAACYDPSKPQTQMQLIHRINPQTTEFCAQLGIIDINVRLQKSKEEHHVCGEYEEQDDVESNDSGEDQSEYNTDTSALSSINPDEIMLDEEEDEDSIVSAHSGMNTPSVEPSDQASEFSASFSDVRILPGSMIVSSDDTVDSTIDREGKPGGTVESGNGEDLTKVPLKRLSDEHEPEQRKKIKRRNQAIYAAVDDDDDDAA

Target ORF information:

RefSeq Version	NM_016216.4
Organism	Homo sapiens(human)
Definition	Homo sapiens debranching RNA lariats 1 (DBR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016216.4

ORF Insert Sequence:

ATGCGGGTGG CTGTGGCTGG CTGCTGCCAC GGCGAGCTGG ATAAGATCTA TGAGACGCTG 
GCGCTGGCAG AGCGGCGCGG CCCGGGGCCT GTCGACCTCT TGCTGTGCTG CGGCGACTTC 
CAGGCGGTGC GCAACGAGGC GGATCTACGC TGCATGGCCG TGCCGCCCAA GTATCGTCAC 
ATGCAAACCT TCTACAGGTA TTACTCTGGA GAGAAAAAGG CTCCAGTTCT CACGCTCTTC 
ATTGGGGGAA ACCATGAAGC CTCAAATCAT TTGCAAGAGT TACCCTATGG TGGCTGGGTG 
GCACCAAACA TTTATTATTT AGGTTTGGCT GGTGTGGTAA AATACCGAGG TGTAAGGATC 
GGTGGAATCT CTGGTATCTT TAAATCTCAT GACTATCGAA AAGGTCATTT TGAGTGCCCC 
CCTTATAATT CATCTACAAT CAGGAGTATA TATCATGTGA GAAATATTGA AGTCTATAAA 
TTAAAACAGC TGAAGCAGCC TATAGATATA TTCTTGTCTC ATGATTGGCC AAGAAGTATA 
TATCATTATG GAAATAAGAA GCAACTTCTT AAGACTAAAT CTTTTTTCCG ACAAGAAGTG 
GAAAATAACA CATTAGGAAG TCCAGCTGCC TCAGAGCTTT TAGAGCATCT CAAACCTACT 
TATTGGTTTT CTGCCCACCT TCATGTGAAG TTTGCCGCCT TGATGCAGCA TCAGGCAAAG 
GATAAAGGAC AGACAGCCAG AGCAACCAAA TTTTTAGCCT TGGACAAATG CTTACCACAT 
AGAGATTTTC TTCAGATATT AGAGATAGAA CATGACCCCA GTGCTCCTGA TTACTTGGAA 
TATGATATTG AATGGCTCAC TATTCTCAGG GCTACGGATG ATCTTATTAA TGTGACTGGG 
CGCCTGTGGA ATATGCCAGA AAATAATGGC CTGCATGCAA GGTGGGATTA TAGTGCAACA 
GAAGAAGGTA TGAAAGAAGT ATTGGAAAAA TTGAATCATG ATCTCAAGGT TCCATGTAAC 
TTTAGTGTAA CAGCTGCTTG TTATGATCCT AGCAAGCCAC AGACACAAAT GCAGCTGATT 
CATAGGATCA ATCCTCAGAC AACTGAATTT TGTGCCCAAC TTGGCATCAT AGACATCAAT 
GTTAGGCTTC AGAAGTCCAA GGAAGAACAT CATGTGTGTG GTGAATATGA AGAACAGGAT 
GATGTGGAGA GTAATGACTC TGGAGAAGAC CAGAGTGAAT ATAATACAGA CACATCTGCT 
CTGTCTTCTA TTAATCCAGA TGAAATAATG TTAGATGAAG AAGAAGATGA AGATAGTATT 
GTAAGTGCAC ATAGTGGCAT GAATACACCA TCGGTAGAAC CTTCTGATCA AGCTTCTGAG 
TTTTCTGCAA GTTTCTCTGA TGTCAGGATC TTGCCAGGCT CTATGATTGT ATCTTCTGAT 
GATACGGTGG ATTCCACAAT TGATAGAGAG GGGAAACCTG GTGGGACTGT GGAGTCAGGG 
AATGGAGAGG ACTTAACCAA GGTGCCATTG AAGAGGCTGA GTGATGAACA TGAACCTGAA 
CAAAGAAAGA AAATTAAGAG GAGGAATCAA GCCATTTACG CTGCAGTGGA TGATGATGAT 
GACGATGCAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBR1 ( NM_016216.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057300.2 Homo sapiens debranching RNA lariats 1 (DBR1), mRNA.

Price & Availability↑

CloneID	OHu11162
Clone ID Related Accession (Same CDS sequence)	NM_016216.4 , NM_016216.3
Accession Version	NM_016216.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	lariat debranching enzyme
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL702928.1, BC009472.1, AC023049.14, CD677175.1 and BF987317.1. On Aug 3, 2010 this sequence version replaced NM_016216.2. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.198817.1, SRR1803614.188181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGTGG CTGTGGCTGG CTGCTGCCAC GGCGAGCTGG ATAAGATCTA TGAGACGCTG 
GCGCTGGCAG AGCGGCGCGG CCCGGGGCCT GTCGACCTCT TGCTGTGCTG CGGCGACTTC 
CAGGCGGTGC GCAACGAGGC GGATCTACGC TGCATGGCCG TGCCGCCCAA GTATCGTCAC 
ATGCAAACCT TCTACAGGTA TTACTCTGGA GAGAAAAAGG CTCCAGTTCT CACGCTCTTC 
ATTGGGGGAA ACCATGAAGC CTCAAATCAT TTGCAAGAGT TACCCTATGG TGGCTGGGTG 
GCACCAAACA TTTATTATTT AGGTTTGGCT GGTGTGGTAA AATACCGAGG TGTAAGGATC 
GGTGGAATCT CTGGTATCTT TAAATCTCAT GACTATCGAA AAGGTCATTT TGAGTGCCCC 
CCTTATAATT CATCTACAAT CAGGAGTATA TATCATGTGA GAAATATTGA AGTCTATAAA 
TTAAAACAGC TGAAGCAGCC TATAGATATA TTCTTGTCTC ATGATTGGCC AAGAAGTATA 
TATCATTATG GAAATAAGAA GCAACTTCTT AAGACTAAAT CTTTTTTCCG ACAAGAAGTG 
GAAAATAACA CATTAGGAAG TCCAGCTGCC TCAGAGCTTT TAGAGCATCT CAAACCTACT 
TATTGGTTTT CTGCCCACCT TCATGTGAAG TTTGCCGCCT TGATGCAGCA TCAGGCAAAG 
GATAAAGGAC AGACAGCCAG AGCAACCAAA TTTTTAGCCT TGGACAAATG CTTACCACAT 
AGAGATTTTC TTCAGATATT AGAGATAGAA CATGACCCCA GTGCTCCTGA TTACTTGGAA 
TATGATATTG AATGGCTCAC TATTCTCAGG GCTACGGATG ATCTTATTAA TGTGACTGGG 
CGCCTGTGGA ATATGCCAGA AAATAATGGC CTGCATGCAA GGTGGGATTA TAGTGCAACA 
GAAGAAGGTA TGAAAGAAGT ATTGGAAAAA TTGAATCATG ATCTCAAGGT TCCATGTAAC 
TTTAGTGTAA CAGCTGCTTG TTATGATCCT AGCAAGCCAC AGACACAAAT GCAGCTGATT 
CATAGGATCA ATCCTCAGAC AACTGAATTT TGTGCCCAAC TTGGCATCAT AGACATCAAT 
GTTAGGCTTC AGAAGTCCAA GGAAGAACAT CATGTGTGTG GTGAATATGA AGAACAGGAT 
GATGTGGAGA GTAATGACTC TGGAGAAGAC CAGAGTGAAT ATAATACAGA CACATCTGCT 
CTGTCTTCTA TTAATCCAGA TGAAATAATG TTAGATGAAG AAGAAGATGA AGATAGTATT 
GTAAGTGCAC ATAGTGGCAT GAATACACCA TCGGTAGAAC CTTCTGATCA AGCTTCTGAG 
TTTTCTGCAA GTTTCTCTGA TGTCAGGATC TTGCCAGGCT CTATGATTGT ATCTTCTGAT 
GATACGGTGG ATTCCACAAT TGATAGAGAG GGGAAACCTG GTGGGACTGT GGAGTCAGGG 
AATGGAGAGG ACTTAACCAA GGTGCCATTG AAGAGGCTGA GTGATGAACA TGAACCTGAA 
CAAAGAAAGA AAATTAAGAG GAGGAATCAA GCCATTTACG CTGCAGTGGA TGATGATGAT 
GACGATGCAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057300.2
CDS	155..1789
Translation	MRVAVAGCCHGELDKIYETLALAERRGPGPVDLLLCCGDFQAVRNEADLRCMAVPPKYRHMQTFYRYYSGEKKAPVLTLFIGGNHEASNHLQELPYGGWVAPNIYYLGLAGVVKYRGVRIGGISGIFKSHDYRKGHFECPPYNSSTIRSIYHVRNIEVYKLKQLKQPIDIFLSHDWPRSIYHYGNKKQLLKTKSFFRQEVENNTLGSPAASELLEHLKPTYWFSAHLHVKFAALMQHQAKDKGQTARATKFLALDKCLPHRDFLQILEIEHDPSAPDYLEYDIEWLTILRATDDLINVTGRLWNMPENNGLHARWDYSATEEGMKEVLEKLNHDLKVPCNFSVTAACYDPSKPQTQMQLIHRINPQTTEFCAQLGIIDINVRLQKSKEEHHVCGEYEEQDDVESNDSGEDQSEYNTDTSALSSINPDEIMLDEEEDEDSIVSAHSGMNTPSVEPSDQASEFSASFSDVRILPGSMIVSSDDTVDSTIDREGKPGGTVESGNGEDLTKVPLKRLSDEHEPEQRKKIKRRNQAIYAAVDDDDDDAA

Target ORF information:

RefSeq Version	NM_016216.3
Organism	Homo sapiens(human)
Definition	Homo sapiens debranching RNA lariats 1 (DBR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016216.3

ORF Insert Sequence:

ATGCGGGTGG CTGTGGCTGG CTGCTGCCAC GGCGAGCTGG ATAAGATCTA TGAGACGCTG 
GCGCTGGCAG AGCGGCGCGG CCCGGGGCCT GTCGACCTCT TGCTGTGCTG CGGCGACTTC 
CAGGCGGTGC GCAACGAGGC GGATCTACGC TGCATGGCCG TGCCGCCCAA GTATCGTCAC 
ATGCAAACCT TCTACAGGTA TTACTCTGGA GAGAAAAAGG CTCCAGTTCT CACGCTCTTC 
ATTGGGGGAA ACCATGAAGC CTCAAATCAT TTGCAAGAGT TACCCTATGG TGGCTGGGTG 
GCACCAAACA TTTATTATTT AGGTTTGGCT GGTGTGGTAA AATACCGAGG TGTAAGGATC 
GGTGGAATCT CTGGTATCTT TAAATCTCAT GACTATCGAA AAGGTCATTT TGAGTGCCCC 
CCTTATAATT CATCTACAAT CAGGAGTATA TATCATGTGA GAAATATTGA AGTCTATAAA 
TTAAAACAGC TGAAGCAGCC TATAGATATA TTCTTGTCTC ATGATTGGCC AAGAAGTATA 
TATCATTATG GAAATAAGAA GCAACTTCTT AAGACTAAAT CTTTTTTCCG ACAAGAAGTG 
GAAAATAACA CATTAGGAAG TCCAGCTGCC TCAGAGCTTT TAGAGCATCT CAAACCTACT 
TATTGGTTTT CTGCCCACCT TCATGTGAAG TTTGCCGCCT TGATGCAGCA TCAGGCAAAG 
GATAAAGGAC AGACAGCCAG AGCAACCAAA TTTTTAGCCT TGGACAAATG CTTACCACAT 
AGAGATTTTC TTCAGATATT AGAGATAGAA CATGACCCCA GTGCTCCTGA TTACTTGGAA 
TATGATATTG AATGGCTCAC TATTCTCAGG GCTACGGATG ATCTTATTAA TGTGACTGGG 
CGCCTGTGGA ATATGCCAGA AAATAATGGC CTGCATGCAA GGTGGGATTA TAGTGCAACA 
GAAGAAGGTA TGAAAGAAGT ATTGGAAAAA TTGAATCATG ATCTCAAGGT TCCATGTAAC 
TTTAGTGTAA CAGCTGCTTG TTATGATCCT AGCAAGCCAC AGACACAAAT GCAGCTGATT 
CATAGGATCA ATCCTCAGAC AACTGAATTT TGTGCCCAAC TTGGCATCAT AGACATCAAT 
GTTAGGCTTC AGAAGTCCAA GGAAGAACAT CATGTGTGTG GTGAATATGA AGAACAGGAT 
GATGTGGAGA GTAATGACTC TGGAGAAGAC CAGAGTGAAT ATAATACAGA CACATCTGCT 
CTGTCTTCTA TTAATCCAGA TGAAATAATG TTAGATGAAG AAGAAGATGA AGATAGTATT 
GTAAGTGCAC ATAGTGGCAT GAATACACCA TCGGTAGAAC CTTCTGATCA AGCTTCTGAG 
TTTTCTGCAA GTTTCTCTGA TGTCAGGATC TTGCCAGGCT CTATGATTGT ATCTTCTGAT 
GATACGGTGG ATTCCACAAT TGATAGAGAG GGGAAACCTG GTGGGACTGT GGAGTCAGGG 
AATGGAGAGG ACTTAACCAA GGTGCCATTG AAGAGGCTGA GTGATGAACA TGAACCTGAA 
CAAAGAAAGA AAATTAAGAG GAGGAATCAA GCCATTTACG CTGCAGTGGA TGATGATGAT 
GACGATGCAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conformational Changes in the 5' End of the HIV-1 Genome Dependent on the Debranching Enzyme DBR1 during Early Stages of Infection.
Journal of virology91(23)(2017 Dec)
Galvis AE,Fisher HE,Fan H,Camerini D

Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development.
Oncogene36(38)5382-5391(2017 Sep)
Han B,Park HK,Ching T,Panneerselvam J,Wang H,Shen Y,Zhang J,Li L,Che R,Garmire L,Fei P

In silico functional dissection of saturation mutagenesis: Interpreting the relationship between phenotypes and changes in protein stability, interactions and activity.
Scientific reports619848(2016 Jan)
Pires DE,Chen J,Blundell TL,Ascher DB

Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.
International journal of molecular sciences16(2)3705-21(2015 Feb)
Masaki S,Yoshimoto R,Kaida D,Hata A,Satoh T,Ohno M,Kataoka N

Impairment of HIV-1 cDNA synthesis by DBR1 knockdown.
Journal of virology88(12)7054-69(2014 Jun)
Galvis AE,Fisher HE,Nitta T,Fan H,Camerini D

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DBR1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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