gRNAs
The following Dbr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dbr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu08605 | |
| Clone ID Related Accession (Same CDS sequence) | NM_031403.3 | |
| Accession Version | NM_031403.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-26 | |
| Organism | Mus musculus(house mouse) | |
| Product | lariat debranching enzyme isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK050090.1. On Apr 6, 2007 this sequence version replaced NM_031403.2. ##Evidence-Data-START## Transcript exon combination :: AK050090.1, SRR1660817.238253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAGGGTGG CCGTGGCCGG CTGCTGCCAC GGCGAGCTGG ACAAGATCTA CGAGACTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_113580.2 |
| CDS | 131..1783 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_031403.3 |
1 | ATGAGGGTGG CCGTGGCCGG CTGCTGCCAC GGCGAGCTGG ACAAGATCTA CGAGACTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu43059 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006511563.2 , NM_001368298.1 | |
| Accession Version | NM_001368298.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-25 | |
| Organism | Mus musculus(house mouse) | |
| Product | lariat debranching enzyme isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC156497.2. On Feb 5, 2019 this sequence version replaced XM_006511563.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.4466444.1, SRR7345562.2844955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAGGGTGG CCGTGGCCGG CTGCTGCCAC GGCGAGCTGG ACAAGATCTA CGAGACTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001355227.1 |
| CDS | 144..1715 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001368298.1 |
1 | ATGAGGGTGG CCGTGGCCGG CTGCTGCCAC GGCGAGCTGG ACAAGATCTA CGAGACTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu43059 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006511563.2 , NM_001368298.1 | |
| Accession Version | XM_006511563.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | lariat debranching enzyme isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006511563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGGGTGG CCGTGGCCGG CTGCTGCCAC GGCGAGCTGG ACAAGATCTA CGAGACTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006511626.1 |
| CDS | 155..1726 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006511563.2 |
1 | ATGAGGGTGG CCGTGGCCGG CTGCTGCCAC GGCGAGCTGG ACAAGATCTA CGAGACTCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. |
Cloning, expression, and complementation test of the RNA lariat debranching enzyme cDNA from mouse. |
Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants. |
