NDUFA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFA2
Entrez Gene ID	4695
Full Name	NADH:ubiquinone oxidoreductase subunit A2
Synonyms	B8,CD14,CIB8
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit A2 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa NADH-ubiquinone oxidoreductase subunit CI-B8 complex I B8 subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010].
Disorder MIM:	602137
Disorder Html:	Leigh syndrome due to mitochondrial complex I deficiency, 256000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002488.5	NP_002479.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 isoform 1
NM_002488.4	NP_002479.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 isoform 1
NM_001185012.1	NP_001171941.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ndufa2	NP_035015.2
Arabidopsis thaliana (thale cress)	AT5G47890	NP_199600.1
Xenopus tropicalis (tropical clawed frog)	ndufa2	NP_001096247.1
Homo sapiens (human)	NDUFA2	NP_002479.1
Oryza sativa (rice)	Os04g0310500	NP_001052435.2
Canis lupus familiaris (dog)	NDUFA2	XP_535211.1
Rattus norvegicus (Norway rat)	Ndufa2	NP_001099623.1
Gallus gallus (chicken)	NDUFA2	XP_001232016.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003367	XP_314269.3
Pan troglodytes (chimpanzee)	NDUFA2	XP_517976.1
Bos taurus (cattle)	NDUFA2	NP_787009.1
Danio rerio (zebrafish)	ndufa2	NP_001019591.1
Drosophila melanogaster (fruit fly)	CG15434	NP_652341.1
	NDUFA2	XP_001087963.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012823	XP_307497.3

Gene Ontology
Bibliography

Related articles in PubMed

Recessive mutations in NDUFA2 cause mitochondrial leukoencephalopathy.
Perrier S, Gauquelin L, T?treault M, Tran LT, Webb N, Srour M, Mitchell JJ, Brunel-Guitton C, Majewski J, Long V, Keller S, Gambello MJ, Simons C, , Vanderver A, Bernard G
Clinical genetics(2017 Aug)

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Stroud DA, Surgenor EE, Formosa LE, Reljic B, Frazier AE, Dibley MG, Osellame LD, Stait T, Beilharz TH, Thorburn DR, Salim A, Ryan MT
Nature538(7623)123-126(2016 Oct)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Internalization and coreceptor expression are critical for TLR2-mediated recognition of lipoteichoic acid in human peripheral blood.
Bunk S, Sigel S, Metzdorf D, Sharif O, Triantafilou K, Triantafilou M, Hartung T, Knapp S, von Aulock S
Journal of immunology (Baltimore, Md. : 1950)185(6)3708-17(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

A novel mechanism of lipoteichoic acid (LTA)-induced cytokine induction in human peripheral blood cells involves uptake of LTA and subsequent intracellular recognition driven by Toll-like receptor (TLR)2, TLR6, and CD14.
Title: Internalization and coreceptor expression are critical for TLR2-mediated recognition of lipoteichoic acid in human peripheral blood.

solution structure shows a thioredoxin fold with highest similarities to the human thioredoxin mutant C73S and thioredoxin 2 from Anabeana sp
Title: The oxidized subunit B8 from human complex I adopts a thioredoxin fold.

The following NDUFA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20548	NM_002488.5 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10742	NM_001185012.1 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu20548	NM_002488.4	Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFA2 ( NM_002488.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002479.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20548
Clone ID Related Accession (Same CDS sequence)	NM_002488.5 , NM_002488.4
Accession Version	NM_002488.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC003674.1, AA993683.1 and AC116353.2. On Nov 30, 2019 this sequence version replaced NM_002488.4. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003674.1, BJ995924.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CCGCAGCAAG TCGAGGAGTC GGGGCAAAGC TGGGCCTGCG TGAGATTCGC 
ATCCACTTAT GTCAGCGCTC GCCCGGCAGC CAGGGCGTCA GGGACTTCAT TGAGAAACGC 
TACGTGGAGC TGAAGAAGGC GAATCCCGAC CTACCCATCC TAATCCGCGA ATGCTCCGAT 
GTGCAGCCCA AGCTCTGGGC CCGCTACGCA TTTGGCCAAG AGACGAATGT CCCTTTGAAC 
AACTTCAGTG CTGATCAGGT AACCAGAGCC CTGGAGAACG TTCTAAGTGG TAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002479.1
CDS	48..347
Translation	MAAAAASRGVGAKLGLREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQETNVPLNNFSADQVTRALENVLSGKA

Target ORF information:

RefSeq Version	NM_002488.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002488.5

ORF Insert Sequence:

ATGGCGGCGG CCGCAGCAAG TCGAGGAGTC GGGGCAAAGC TGGGCCTGCG TGAGATTCGC 
ATCCACTTAT GTCAGCGCTC GCCCGGCAGC CAGGGCGTCA GGGACTTCAT TGAGAAACGC 
TACGTGGAGC TGAAGAAGGC GAATCCCGAC CTACCCATCC TAATCCGCGA ATGCTCCGAT 
GTGCAGCCCA AGCTCTGGGC CCGCTACGCA TTTGGCCAAG AGACGAATGT CCCTTTGAAC 
AACTTCAGTG CTGATCAGGT AACCAGAGCC CTGGAGAACG TTCTAAGTGG TAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFA2 ( NM_002488.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002479.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20548
Clone ID Related Accession (Same CDS sequence)	NM_002488.5 , NM_002488.4
Accession Version	NM_002488.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116353.2, BC003674.1 and AA993683.1. This sequence is a reference standard in the RefSeqGene project. On May 29, 2010 this sequence version replaced NM_002488.3. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AA993683.1, BI546882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CCGCAGCAAG TCGAGGAGTC GGGGCAAAGC TGGGCCTGCG TGAGATTCGC 
ATCCACTTAT GTCAGCGCTC GCCCGGCAGC CAGGGCGTCA GGGACTTCAT TGAGAAACGC 
TACGTGGAGC TGAAGAAGGC GAATCCCGAC CTACCCATCC TAATCCGCGA ATGCTCCGAT 
GTGCAGCCCA AGCTCTGGGC CCGCTACGCA TTTGGCCAAG AGACGAATGT CCCTTTGAAC 
AACTTCAGTG CTGATCAGGT AACCAGAGCC CTGGAGAACG TTCTAAGTGG TAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002479.1
CDS	203..502
Translation	MAAAAASRGVGAKLGLREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYAFGQETNVPLNNFSADQVTRALENVLSGKA

Target ORF information:

RefSeq Version	NM_002488.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002488.4

ORF Insert Sequence:

ATGGCGGCGG CCGCAGCAAG TCGAGGAGTC GGGGCAAAGC TGGGCCTGCG TGAGATTCGC 
ATCCACTTAT GTCAGCGCTC GCCCGGCAGC CAGGGCGTCA GGGACTTCAT TGAGAAACGC 
TACGTGGAGC TGAAGAAGGC GAATCCCGAC CTACCCATCC TAATCCGCGA ATGCTCCGAT 
GTGCAGCCCA AGCTCTGGGC CCGCTACGCA TTTGGCCAAG AGACGAATGT CCCTTTGAAC 
AACTTCAGTG CTGATCAGGT AACCAGAGCC CTGGAGAACG TTCTAAGTGG TAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFA2 ( NM_001185012.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171941.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10742
Clone ID Related Accession (Same CDS sequence)	NM_001185012.1
Accession Version	NM_001185012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116353.2, BQ054127.1 and AA993683.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1. This results in a protein with a shorter and distinct C-terminus (isoform 2), compared to isoform 1. It is unknown if isoform 2 is located to the mitochondria and/or if it is functional. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.34326.1, AV705564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CCGCAGCAAG TCGAGGAGTC GGGGCAAAGC TGGGCCTGCG TGAGATTCGC 
ATCCACTTAT GTCAGCGCTC GCCCGGCAGC CAGGGCGTCA GGGACTTCAT TGAGAAACGC 
TACGTGGAGC TGAAGAAGGC GAATCCCGAC CTACCCATCC TAATCCGCGA ATGCTCCGAT 
GTGCAGCCCA AGCTCTGGGC CCGCTACGCC TCCAGGGTGC AGAATAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171941.1
CDS	203..433
Translation	MAAAAASRGVGAKLGLREIRIHLCQRSPGSQGVRDFIEKRYVELKKANPDLPILIRECSDVQPKLWARYASRVQNS

Target ORF information:

RefSeq Version	NM_001185012.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit A2 (NDUFA2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185012.1

ORF Insert Sequence:

ATGGCGGCGG CCGCAGCAAG TCGAGGAGTC GGGGCAAAGC TGGGCCTGCG TGAGATTCGC 
ATCCACTTAT GTCAGCGCTC GCCCGGCAGC CAGGGCGTCA GGGACTTCAT TGAGAAACGC 
TACGTGGAGC TGAAGAAGGC GAATCCCGAC CTACCCATCC TAATCCGCGA ATGCTCCGAT 
GTGCAGCCCA AGCTCTGGGC CCGCTACGCC TCCAGGGTGC AGAATAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Recessive mutations in NDUFA2 cause mitochondrial leukoencephalopathy.
Clinical genetics(2017 Aug)
Perrier S,Gauquelin L,T?treault M,Tran LT,Webb N,Srour M,Mitchell JJ,Brunel-Guitton C,Majewski J,Long V,Keller S,Gambello MJ,Simons C,,Vanderver A,Bernard G

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Nature538(7623)123-126(2016 Oct)
Stroud DA,Surgenor EE,Formosa LE,Reljic B,Frazier AE,Dibley MG,Osellame LD,Stait T,Beilharz TH,Thorburn DR,Salim A,Ryan MT

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Internalization and coreceptor expression are critical for TLR2-mediated recognition of lipoteichoic acid in human peripheral blood.
Journal of immunology (Baltimore, Md. : 1950)185(6)3708-17(2010 Sep)
Bunk S,Sigel S,Metzdorf D,Sharif O,Triantafilou K,Triantafilou M,Hartung T,Knapp S,von Aulock S

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NDUFA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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