Ndufa2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndufa2
Entrez Gene ID	17991
Full Name	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2
Synonyms	AV000592,B8,C1-B8,CI-B8
General protein information	Preferred Names NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 NADH-ubiquinone oxidoreductase B8 subunit complex I-B8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18\|18 B2
Summary	This gene encodes a subunit of the NADH-ubiquinone oxidoreductase (complex I) enzyme, which is a large, multimeric protein. It is the first enzyme complex in the mitochondrial electron transport chain and catalyzes the transfer of electrons from NADH to the electron acceptor ubiquinone. The proton gradient created by electron transfer drives the conversion of ADP to ATP. The human ortholog of this gene has been characterized, and its structure and redox potential is reported to be similar to that of thioredoxins. It may be involved in regulating complex I activity or assembly via assistance in redox processes. In humans, mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. A pseudogene of this gene is located on chromosome 5. [provided by RefSeq, May 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_010885.5	NP_035015.2	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ndufa2	NP_035015.2
Arabidopsis thaliana (thale cress)	AT5G47890	NP_199600.1
Xenopus tropicalis (tropical clawed frog)	ndufa2	NP_001096247.1
Homo sapiens (human)	NDUFA2	NP_002479.1
Oryza sativa (rice)	Os04g0310500	NP_001052435.2
Canis lupus familiaris (dog)	NDUFA2	XP_535211.1
Rattus norvegicus (Norway rat)	Ndufa2	NP_001099623.1
Gallus gallus (chicken)	NDUFA2	XP_001232016.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003367	XP_314269.3
Pan troglodytes (chimpanzee)	NDUFA2	XP_517976.1
Bos taurus (cattle)	NDUFA2	NP_787009.1
Danio rerio (zebrafish)	ndufa2	NP_001019591.1
Drosophila melanogaster (fruit fly)	CG15434	NP_652341.1
	NDUFA2	XP_001087963.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012823	XP_307497.3

Gene Ontology
Bibliography

Related articles in PubMed

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Cui W, Dai X, Marcho C, Han Z, Zhang K, Tremblay KD, Mager J
Scientific reports637396(2016 Nov)

Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons.
Doucet-Beaupr? H, Gilbert C, Profes MS, Chabrat A, Pacelli C, Gigu?re N, Rioux V, Charest J, Deng Q, Laguna A, Ericson J, Perlmann T, Ang SL, Cicchetti F, Parent M, Trudeau LE, L?vesque M
Proceedings of the National Academy of Sciences of the United States of America113(30)E4387-96(2016 07)

IGF2BP2/IMP2-Deficient mice resist obesity through enhanced translation of Ucp1 mRNA and Other mRNAs encoding mitochondrial proteins.
Dai N, Zhao L, Wrighting D, Kr?mer D, Majithia A, Wang Y, Cracan V, Borges-Rivera D, Mootha VK, Nahrendorf M, Thorburn DR, Minichiello L, Altshuler D, Avruch J
Cell metabolism21(4)609-21(2015 Apr)

Subunit-specific incorporation efficiency and kinetics in mitochondrial complex I homeostasis.
Dieteren CE, Koopman WJ, Swarts HG, Peters JG, Maczuga P, van Gemst JJ, Masereeuw R, Smeitink JA, Nijtmans LG, Willems PH
The Journal of biological chemistry287(50)41851-60(2012 Dec)

Region-specific expression of mitochondrial complex I genes during murine brain development.
Wirtz S, Schuelke M
PloS one6(4)e18897(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors use green fluorescent-tagged complex I subunits to determine the kinetics of assembly. Tagged human NDUFA2 may incorporate into the holo-complex I enzyme as an assembly intermediate or as free monomers. The authors conclude that nuclear-encoded subunits incorporate into the holo-enzyme in a subunit-specific manner.
Title: Subunit-specific incorporation efficiency and kinetics in mitochondrial complex I homeostasis.

The authors determine the NMR structure of the oxidized human NDUFA2 protein, also known as B8, and report that its structure and redox potential is similar to that of thioredoxins. The highly conserved residues on the surface most likely form the protein-protein interaction site of this subunit with the large multimeric complex I.
Title: The oxidized subunit B8 from human complex I adopts a thioredoxin fold.

differentially expressed in the brains of four mice strains; expression pattern follows ethanol preference of the strains [(NADH-ubiquinone oxidoreductase B8 subunit]
Title: Genetics and differential expression of NADH:ubiquinone oxidoreductase B8 subunit in brains of genetic strains of mice differing in voluntary alcohol consumption.

The following Ndufa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16401	NM_010885.5 Latest version!	Mus musculus NADH:ubiquinone oxidoreductase subunit A2 (Ndufa2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufa2 ( NM_010885.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035015.2 Mus musculus NADH:ubiquinone oxidoreductase subunit A2 (Ndufa2), mRNA.

Price & Availability↑

CloneID	OMu16401
Clone ID Related Accession (Same CDS sequence)	NM_010885.5
Accession Version	NM_010885.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV482131.1, AK003608.1 and AC027740.11. On May 30, 2013 this sequence version replaced NM_010885.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ746689.1, CD769118.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 23038253; reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTG CCGCTGCTAG CCGAGCGGTC GGCGCAAAGC TGGGGTTGCG TGAGATTCGC 
GTTCACTTAT GCCAGCGTTC CCCAGGCAGC CAGGGTGTGA GGGATTTCAT CGTGCAACGG 
TACGTGGAGC TGAAGAAGGC GCACCCCAAC CTGCCCATTC TGATCCGCGA ATGCTCGGAG 
GTGCAGCCCA AGCTTTGGGC CCGCTATGCT TTTGGCCAAG AGAAGACGGT GTCTCTGAAC 
AATCTGAGTG CTGATGAGGT AACCAGAGCC ATGCAGAATG TGCTAAGCGG CAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035015.2
CDS	68..367
Translation	MAAAAASRAVGAKLGLREIRVHLCQRSPGSQGVRDFIVQRYVELKKAHPNLPILIRECSEVQPKLWARYAFGQEKTVSLNNLSADEVTRAMQNVLSGKA

Target ORF information:

RefSeq Version	NM_010885.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus NADH:ubiquinone oxidoreductase subunit A2 (Ndufa2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010885.5

ORF Insert Sequence:

ATGGCGGCTG CCGCTGCTAG CCGAGCGGTC GGCGCAAAGC TGGGGTTGCG TGAGATTCGC 
GTTCACTTAT GCCAGCGTTC CCCAGGCAGC CAGGGTGTGA GGGATTTCAT CGTGCAACGG 
TACGTGGAGC TGAAGAAGGC GCACCCCAAC CTGCCCATTC TGATCCGCGA ATGCTCGGAG 
GTGCAGCCCA AGCTTTGGGC CCGCTATGCT TTTGGCCAAG AGAAGACGGT GTCTCTGAAC 
AATCTGAGTG CTGATGAGGT AACCAGAGCC ATGCAGAATG TGCTAAGCGG CAAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Towards Functional Annotation of the Preimplantation Transcriptome: An RNAi Screen in Mammalian Embryos.
Scientific reports637396(2016 Nov)
Cui W,Dai X,Marcho C,Han Z,Zhang K,Tremblay KD,Mager J

Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons.
Proceedings of the National Academy of Sciences of the United States of America113(30)E4387-96(2016 07)
Doucet-Beaupr? H,Gilbert C,Profes MS,Chabrat A,Pacelli C,Gigu?re N,Rioux V,Charest J,Deng Q,Laguna A,Ericson J,Perlmann T,Ang SL,Cicchetti F,Parent M,Trudeau LE,L?vesque M

IGF2BP2/IMP2-Deficient mice resist obesity through enhanced translation of Ucp1 mRNA and Other mRNAs encoding mitochondrial proteins.
Cell metabolism21(4)609-21(2015 Apr)
Dai N,Zhao L,Wrighting D,Kr?mer D,Majithia A,Wang Y,Cracan V,Borges-Rivera D,Mootha VK,Nahrendorf M,Thorburn DR,Minichiello L,Altshuler D,Avruch J

Subunit-specific incorporation efficiency and kinetics in mitochondrial complex I homeostasis.
The Journal of biological chemistry287(50)41851-60(2012 Dec)
Dieteren CE,Koopman WJ,Swarts HG,Peters JG,Maczuga P,van Gemst JJ,Masereeuw R,Smeitink JA,Nijtmans LG,Willems PH

Region-specific expression of mitochondrial complex I genes during murine brain development.
PloS one6(4)e18897(2011 Apr)
Wirtz S,Schuelke M

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Ndufa2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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