RNASEK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNASEK
Entrez Gene ID	440400
Full Name	ribonuclease K
General protein information	Preferred Names ribonuclease K Names ribonuclease kappa RNase kappa RNase kappa-02 isoform ribonuclease, RNase K
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Disorder MIM:	617098

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004333.4	NP_001004333.2	ribonuclease kappa

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RNASEK	XP_003315387.1
Bos taurus (cattle)	RNASEK	NP_001029601.1
Mus musculus (house mouse)	Rnasek	NP_776103.1
Danio rerio (zebrafish)	LOC100534769	XP_003199388.1
Danio rerio (zebrafish)	rnaseka	NP_001038870.1
Homo sapiens (human)	RNASEK	NP_001004333.2
Rattus norvegicus (Norway rat)	Rnasek	NP_001131033.2
Danio rerio (zebrafish)	rnasekb	NP_001038840.1
	RNASEK	XP_002800310.1
Canis lupus familiaris (dog)	RNASEK	XP_005619993.1
Xenopus tropicalis (tropical clawed frog)	LOC100493134	XP_002940100.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of SLC16A11 Variants in 12,811 American Indians: Genotype-Obesity Interaction for Type 2 Diabetes and an Association With RNASEK Expression.
Traurig M, Hanson RL, Marinelarena A, Kobes S, Piaggi P, Cole S, Curran JE, Blangero J, G?ring H, Kumar S, Nelson RG, Howard BV, Knowler WC, Baier LJ, Bogardus C
Diabetes65(2)510-9(2016 Feb)

RNASEK Is a V-ATPase-Associated Factor Required for Endocytosis and the Replication of Rhinovirus, Influenza A Virus, and Dengue Virus.
Perreira JM, Aker AM, Savidis G, Chin CR, McDougall WM, Portmann JM, Meraner P, Smith MC, Rahman M, Baker RE, Gauthier A, Franti M, Brass AL
Cell reports12(5)850-63(2015 Aug)

An atlas of genetic influences on human blood metabolites.
Shin SY, Fauman EB, Petersen AK, Krumsiek J, Santos R, Huang J, Arnold M, Erte I, Forgetta V, Yang TP, Walter K, Menni C, Chen L, Vasquez L, Valdes AM, Hyde CL, Wang V, Ziemek D, Roberts P, Xi L, Grundberg E, , Waldenberger M, Richards JB, Mohney RP, Milburn MV, John SL, Trimmer J, Theis FJ, Overington JP, Suhre K, Brosnan MJ, Gieger C, Kastenm?ller G, Spector TD, Soranzo N
Nature genetics46(6)543-550(2014 Jun)

A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform.
Karousis ED, Sideris DC
PloS one9(5)e96557(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analyses of global gene expression data from adipose tissue, skeletal muscle, and whole blood provide evidence that rs75493593 is associated with RNASEK gene expression, suggesting that RNASEK expression may mediate the effect of genotype on diabetes.
Title: Analysis of SLC16A11 Variants in 12,811 American Indians: Genotype-Obesity Interaction for Type 2 Diabetes and an Association With RNASEK Expression.

RNASEK closely associates with the V-ATPase and is required for its function; its loss prevents the early events of endocytosis and the replication of multiple pathogenic viruses.
Title: RNASEK Is a V-ATPase-Associated Factor Required for Endocytosis and the Replication of Rhinovirus, Influenza A Virus, and Dengue Virus.

Data suggest that the Cys6-Cys69 disulfide bond is likely to exist in all RNase kappa family members, including human.
Title: Essential cysteine residues for human RNase ? catalytic activity.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following RNASEK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNASEK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu10905	NM_001004333.4 Latest version!	Homo sapiens ribonuclease K (RNASEK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNASEK ( NM_001004333.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001004333.2 Homo sapiens ribonuclease K (RNASEK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10905
Clone ID Related Accession (Same CDS sequence)	NM_001004333.4
Accession Version	NM_001004333.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	ribonuclease kappa
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM835723.1, AC040977.10, BC051802.1, BC070349.1, AA633512.1 and CD176288.1. On Jan 26, 2011 this sequence version replaced NM_001004333.3. Transcript Variant: This variant (1) encodes the supported protein. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.388602.1, SRR1163658.260604.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGATGGT TGAGGCCGGG GCCACGCCCC CTCTGCCCCC CTGCGAGGGC ATCCTGGGCT 
TTCTCCCACC GCTTTCCGAG CCCGCTTGCA CCTCGGCGAT CCCCGACTCC CTTCTTTATG 
GCGTCGCTCC TGTGCTGTGG GCCGAAGCTG GCCGCCTGCG GCATCGTCCT CAGCGCCTGG 
GGAGTGATCA TGTTGATAAT GCTCGGAATA TTTTTCAATG TCCATTCCGC TGTGTTGATT 
GAGGACGTTC CCTTCACGGA GAAAGATTTT GAGAATGGCC CCCAGAACAT ATACAACCTT 
TACGAGCAAG TCAGCTACAA CTGTTTCATC GCTGCAGGCC TTTACCTCCT CCTCGGAGGC 
TTCTCTTTCT GCCAAGTTCG GCTCAATAAG CGCAAGGAAT ACATGGTGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004333.2
CDS	151..564
Translation	MGWLRPGPRPLCPPARASWAFSHRFPSPLAPRRSPTPFFMASLLCCGPKLAACGIVLSAWGVIMLIMLGIFFNVHSAVLIEDVPFTEKDFENGPQNIYNLYEQVSYNCFIAAGLYLLLGGFSFCQVRLNKRKEYMVR

Target ORF information:

RefSeq Version	NM_001004333.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonuclease K (RNASEK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004333.4

ORF Insert Sequence:

ATGGGATGGT TGAGGCCGGG GCCACGCCCC CTCTGCCCCC CTGCGAGGGC ATCCTGGGCT 
TTCTCCCACC GCTTTCCGAG CCCGCTTGCA CCTCGGCGAT CCCCGACTCC CTTCTTTATG 
GCGTCGCTCC TGTGCTGTGG GCCGAAGCTG GCCGCCTGCG GCATCGTCCT CAGCGCCTGG 
GGAGTGATCA TGTTGATAAT GCTCGGAATA TTTTTCAATG TCCATTCCGC TGTGTTGATT 
GAGGACGTTC CCTTCACGGA GAAAGATTTT GAGAATGGCC CCCAGAACAT ATACAACCTT 
TACGAGCAAG TCAGCTACAA CTGTTTCATC GCTGCAGGCC TTTACCTCCT CCTCGGAGGC 
TTCTCTTTCT GCCAAGTTCG GCTCAATAAG CGCAAGGAAT ACATGGTGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of SLC16A11 Variants in 12,811 American Indians: Genotype-Obesity Interaction for Type 2 Diabetes and an Association With RNASEK Expression.
Diabetes65(2)510-9(2016 Feb)
Traurig M,Hanson RL,Marinelarena A,Kobes S,Piaggi P,Cole S,Curran JE,Blangero J,G?ring H,Kumar S,Nelson RG,Howard BV,Knowler WC,Baier LJ,Bogardus C

RNASEK Is a V-ATPase-Associated Factor Required for Endocytosis and the Replication of Rhinovirus, Influenza A Virus, and Dengue Virus.
Cell reports12(5)850-63(2015 Aug)
Perreira JM,Aker AM,Savidis G,Chin CR,McDougall WM,Portmann JM,Meraner P,Smith MC,Rahman M,Baker RE,Gauthier A,Franti M,Brass AL

An atlas of genetic influences on human blood metabolites.
Nature genetics46(6)543-550(2014 Jun)
Shin SY,Fauman EB,Petersen AK,Krumsiek J,Santos R,Huang J,Arnold M,Erte I,Forgetta V,Yang TP,Walter K,Menni C,Chen L,Vasquez L,Valdes AM,Hyde CL,Wang V,Ziemek D,Roberts P,Xi L,Grundberg E,,Waldenberger M,Richards JB,Mohney RP,Milburn MV,John SL,Trimmer J,Theis FJ,Overington JP,Suhre K,Brosnan MJ,Gieger C,Kastenm?ller G,Spector TD,Soranzo N

A subtle alternative splicing event gives rise to a widely expressed human RNase k isoform.
PloS one9(5)e96557(2014)
Karousis ED,Sideris DC

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RNASEK cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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