MID1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID1
Entrez Gene ID	4281
Full Name	midline 1
Synonyms	BBBG1,FXY,GBBB1,MIDIN,OGS1,OS,OSX,RNF59,TRIM18,XPRF,ZNFXY
General protein information	Preferred Names midline 1 Names E3 ubiquitin-protein ligase Midline-1 Opitz/BBB syndrome RING finger protein 59 RING finger protein Midline-1 RING-type E3 ubiquitin transferase Midline-1 midline 1 RING finger protein putative transcription factor XPRF tripartite motif protein TRIM18 tripartite motif-containing protein 18 zinc finger on X and Y, mouse, homolog of
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp22.2
Summary	The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016].
Disorder MIM:	300552
Disorder Html:	Opitz GBBB syndrome, type I, 300000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005274537.1	XP_005274594.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_017029534.1	XP_016885023.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
NM_001347733.1	NP_001334662.1	E3 ubiquitin-protein ligase Midline-1 isoform 1
NM_033289.1	NP_150631.1	E3 ubiquitin-protein ligase Midline-1 isoform 2
NM_001193279.1	NP_001180208.1	E3 ubiquitin-protein ligase Midline-1 isoform 4
NM_001193278.1	NP_001180207.1	E3 ubiquitin-protein ligase Midline-1 isoform 3
NM_033290.4	NP_150632.1	E3 ubiquitin-protein ligase Midline-1 isoform 1
NM_033289.2	NP_150631.1	E3 ubiquitin-protein ligase Midline-1 isoform 2
XM_006724492.3	XP_006724555.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_017029535.1	XP_016885024.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
XM_017029537.1	XP_016885026.1	E3 ubiquitin-protein ligase Midline-1 isoform X4
XM_017029541.1	XP_016885030.1	E3 ubiquitin-protein ligase Midline-1 isoform X6
NM_001193281.1	NP_001180210.1	E3 ubiquitin-protein ligase Midline-1 isoform 6
XM_011545525.2	XP_011543827.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_011545526.2	XP_011543828.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_011545527.2	XP_011543829.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
NM_001193280.1	NP_001180209.1	E3 ubiquitin-protein ligase Midline-1 isoform 5
NM_001098624.2	NP_001092094.1	E3 ubiquitin-protein ligase Midline-1 isoform 1
NM_001347733.2	NP_001334662.1	E3 ubiquitin-protein ligase Midline-1 isoform 1
XM_005274536.1	XP_005274593.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_017029542.1	XP_016885031.1	E3 ubiquitin-protein ligase Midline-1 isoform X7
NM_000381.3	NP_000372.1	E3 ubiquitin-protein ligase Midline-1 isoform 1
NM_001193277.1	NP_001180206.1	E3 ubiquitin-protein ligase Midline-1 isoform 1
NM_033290.3	NP_150632.1	E3 ubiquitin-protein ligase Midline-1 isoform 1
XM_017029538.1	XP_016885027.1	E3 ubiquitin-protein ligase Midline-1 isoform X4
XM_017029539.1	XP_016885028.1	E3 ubiquitin-protein ligase Midline-1 isoform X4
XM_017029540.1	XP_016885029.1	E3 ubiquitin-protein ligase Midline-1 isoform X5
XM_017029536.1	XP_016885025.1	E3 ubiquitin-protein ligase Midline-1 isoform X3
NM_000381.4	NP_000372.1	E3 ubiquitin-protein ligase Midline-1 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mid1	NP_034927.2
Homo sapiens (human)	MID1	NP_150632.1
Rattus norvegicus (Norway rat)	Mid1	NP_075216.1
Danio rerio (zebrafish)	mid1	XP_002663478.1
Xenopus tropicalis (tropical clawed frog)	mid1	NP_001006876.1
Bos taurus (cattle)	MID1	NP_001179751.1
Gallus gallus (chicken)	MID1	NP_989460.1
	MID1	XP_001093390.1
Canis lupus familiaris (dog)	MID1	XP_548857.2

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNAs miR-19, miR-340, miR-374 and miR-542 regulate MID1 protein expression.
Unterbruner K, Matthes F, Schilling J, Nalavade R, Weber S, Winter J, Krau? S
PloS one13(1)e0190437(2018)

The E3 ubiquitin ligase MID1/TRIM18 promotes atypical ubiquitination of the BRCA2-associated factor 35, BRAF35.
Zanchetta ME, Napolitano LMR, Maddalo D, Meroni G
Biochimica et biophysica acta1864(10)1844-1854(2017 10)

Structural and functional observations of the P151L MID1 mutation reveal alpha4 plays a significant role in X-linked Opitz Syndrome.
Wright KM, Du H, Massiah MA
The FEBS journal284(14)2183-2193(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data reveal a novel role for MID1 and for atypical ubiquitination in balancing BRAF35 presence, and likely its activity, within nuclear and cytoplasmic compartments
Title: The E3 ubiquitin ligase MID1/TRIM18 promotes atypical ubiquitination of the BRCA2-associated factor 35, BRAF35.

P151L MID1 mutation is associated with X-linked Opitz Syndrome.
Title: Structural and functional observations of the P151L MID1 mutation reveal alpha4 plays a significant role in X-linked Opitz Syndrome.

Osx is upregulated in patients with Ossification of the posterior longitudinal ligament.
Title: Ossification of the posterior ligament is mediated by osterix via inhibition of the ?-catenin signaling pathway.

the coiled-coil and COS domain (CC-COS) bind to microtubules, demonstrating for the first time that MID1 can directly associate with the microtubules
Title: Solution structure of the microtubule-targeting COS domain of MID1.

TRAIL regulates MID1 and TSLP, inflammation, fibrosis, smooth muscle hypertrophy, and expression of inflammatory effector chemokines and cytokines in experimental eosinophilic esophagitis.
Title: TNF-related apoptosis-inducing ligand (TRAIL) regulates midline-1, thymic stromal lymphopoietin, inflammation, and remodeling in experimental eosinophilic esophagitis.

The following MID1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28332	NM_001347733.2 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28332	NM_001193277.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28332	NM_000381.4 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28332	NM_033290.4 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28332	NM_001098624.2 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28332	XM_017029536.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28329	NM_001193278.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu28335	NM_001193280.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu28337	NM_001193279.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$286.30 $409.00
OHu28338	NM_033289.2 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu28339	NM_001193281.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu28329	XM_017029541.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu28337	XM_017029542.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$286.30 $409.00
OHu28338	XM_017029537.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu28338	XM_017029539.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu28338	XM_017029538.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu99314	XM_017029540.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu53923	XM_011545527.2 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53923	XM_017029534.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53923	XM_006724492.3 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53923	XM_005274537.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53923	XM_011545525.2 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53923	XM_011545526.2 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53923	XM_005274536.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu53924	XM_017029535.1 Latest version!	Homo sapiens midline 1 (MID1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu28338	NM_033289.1	Homo sapiens midline 1 (MID1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu28332	NM_001347733.1	Homo sapiens midline 1 (MID1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28332	NM_033290.3	Homo sapiens midline 1 (MID1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28332	NM_000381.3	Homo sapiens midline 1 (MID1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID1 ( NM_001347733.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334662.1 Homo sapiens midline 1 (MID1), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu28332
Clone ID Related Accession (Same CDS sequence)	NM_001347733.1 , NM_033290.4 , NM_001098624.2 , NM_001347733.2 , NM_000381.3 , NM_001193277.1 , NM_033290.3 , XM_017029536.1 , NM_000381.4
Accession Version	NM_001347733.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117406.2 and AC008008.2. Transcript Variant: This variant (10) has an alternate 5' UTR exon, as compared to variant 1. Variants 1-4 and variant 10 encode the same isoform (1). This variant represents use of an alternate long terminal repeat (LTR) promoter and exon sequence, and results in placenta expression. (PMID: 12411602). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF041208.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334662.1
CDS	414..2417
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_001347733.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347733.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_033290.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_150632.1 Homo sapiens midline 1 (MID1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28332
Clone ID Related Accession (Same CDS sequence)	NM_001347733.1 , NM_033290.4 , NM_001098624.2 , NM_001347733.2 , NM_000381.3 , NM_001193277.1 , NM_033290.3 , XM_017029536.1 , NM_000381.4
Accession Version	NM_033290.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA856308.1, AF041210.1 and EF217426.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_033290.3. Transcript Variant: This variant (3) has an alternate 5' UTR exon, as compared to variant 1. Variants 1-4 and variant 10 encode the same isoform (1). Expression was found to be adipose specific. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF041210.1, SRR1803612.256969.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_150632.1
CDS	187..2190
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_033290.4
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033290.4

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_001098624.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092094.1 Homo sapiens midline 1 (MID1), transcript variant 4, mRNA.

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CloneID	OHu28332
Clone ID Related Accession (Same CDS sequence)	NM_001347733.1 , NM_033290.4 , NM_001098624.2 , NM_001347733.2 , NM_000381.3 , NM_001193277.1 , NM_033290.3 , XM_017029536.1 , NM_000381.4
Accession Version	NM_001098624.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC369426.1, BC053626.1 and EF217426.1. On Jul 17, 2010 this sequence version replaced NM_001098624.1. Transcript Variant: This variant (4) has an alternate 5' UTR exon, as compared to variant 1. Variants 1-4 and variant 10 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.228960.1, SRR1660807.138328.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092094.1
CDS	332..2335
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_001098624.2
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098624.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_001347733.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001334662.1 Homo sapiens midline 1 (MID1), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu28332
Clone ID Related Accession (Same CDS sequence)	NM_001347733.1 , NM_033290.4 , NM_001098624.2 , NM_001347733.2 , NM_000381.3 , NM_001193277.1 , NM_033290.3 , XM_017029536.1 , NM_000381.4
Accession Version	NM_001347733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117406.2 and AC008008.2. On Jun 2, 2019 this sequence version replaced NM_001347733.1. Transcript Variant: This variant (10) has an alternate 5' UTR exon, as compared to variant 1. Variants 1-4 and variant 10 encode the same isoform (1). This variant represents use of an alternate long terminal repeat (LTR) promoter and exon sequence, and results in placenta expression. (PMID: 12411602). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.2735632.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334662.1
CDS	431..2434
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_001347733.2
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347733.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_000381.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000372.1 Homo sapiens midline 1 (MID1), transcript variant 1, mRNA.

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CloneID	OHu28332
Clone ID Related Accession (Same CDS sequence)	NM_001347733.1 , NM_033290.4 , NM_001098624.2 , NM_001347733.2 , NM_000381.3 , NM_001193277.1 , NM_033290.3 , XM_017029536.1 , NM_000381.4
Accession Version	NM_000381.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC309977.1, BC053626.1 and EF217426.1. On Jul 17, 2010 this sequence version replaced NM_000381.2. Transcript Variant: This variant (1, also known as alpha) is the longest transcript. Variants 1-4 and variant 10 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.16033.1, SRR1660807.78880.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000372.1
CDS	402..2405
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_000381.3
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000381.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_001193277.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180206.1 Homo sapiens midline 1 (MID1), transcript variant 2, mRNA.

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CloneID	OHu28332
Clone ID Related Accession (Same CDS sequence)	NM_001347733.1 , NM_033290.4 , NM_001098624.2 , NM_001347733.2 , NM_000381.3 , NM_001193277.1 , NM_033290.3 , XM_017029536.1 , NM_000381.4
Accession Version	NM_001193277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA435613.1, AF035360.1 and EF217426.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon, as compared to variant 1. Variants 1-4 and variant 10 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.82712.1, AF035360.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180206.1
CDS	191..2194
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_001193277.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193277.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_033290.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_150632.1 Homo sapiens midline 1 (MID1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28332
Clone ID Related Accession (Same CDS sequence)	NM_001347733.1 , NM_033290.4 , NM_001098624.2 , NM_001347733.2 , NM_000381.3 , NM_001193277.1 , NM_033290.3 , XM_017029536.1 , NM_000381.4
Accession Version	NM_033290.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA856308.1, AF041210.1 and EF217426.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2010 this sequence version replaced NM_033290.2. Transcript Variant: This variant (3) has an alternate 5' UTR exon, as compared to variant 1. Variants 1-4 and variant 10 encode the same isoform (1). Expression was found to be adipose specific. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC053626.1, SRR1803612.80173.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_150632.1
CDS	194..2197
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_033290.3
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033290.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_000381.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000372.1 Homo sapiens midline 1 (MID1), transcript variant 1, mRNA.

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CloneID	OHu28332
Clone ID Related Accession (Same CDS sequence)	NM_001347733.1 , NM_033290.4 , NM_001098624.2 , NM_001347733.2 , NM_000381.3 , NM_001193277.1 , NM_033290.3 , XM_017029536.1 , NM_000381.4
Accession Version	NM_000381.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC309977.1, BC053626.1 and EF217426.1. On Jul 1, 2019 this sequence version replaced NM_000381.3. Transcript Variant: This variant (1, also known as alpha) is the longest transcript. Variants 1-4 and variant 10 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.16033.1, SRR1660807.78880.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000372.1
CDS	353..2356
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_000381.4
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000381.4

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_017029536.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885025.1 Homo sapiens midline 1 (MID1), transcript variant X9, mRNA.

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CloneID	OHu28332
Clone ID Related Accession (Same CDS sequence)	NM_001347733.1 , NM_033290.4 , NM_001098624.2 , NM_001347733.2 , NM_000381.3 , NM_001193277.1 , NM_033290.3 , XM_017029536.1 , NM_000381.4
Accession Version	XM_017029536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885025.1
CDS	630..2633
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_017029536.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029536.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTATG CCATTGGTCT TGCTTACAAA 
TCAGCCCCGA AGCATGAATG GATTGGGAAG AACTCTGCTT CCTGGGCGCT CTGCCGCTGC 
AACAATAACT GGGTGGTGAG ACACAATAGC AAGGAAATCC CCATTGAGCC TGCCCCCCAC 
CTCCGGCGCG TGGGCATCCT GCTGGACTAT GATAACGGCT CTATCGCCTT TTATGATGCT 
TTGAACTCCA TCCACCTCTA CACCTTCGAC GTCGCATTTG CGCAGCCTGT TTGCCCCACC 
TTCACCGTGT GGAACAAGTG TCTGACGATT ATCACTGGGC TCCCTATCCC AGACCATTTG 
GACTGCACAG AGCAGCTGCC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_001193278.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180207.1 Homo sapiens midline 1 (MID1), transcript variant 6, mRNA.

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CloneID	OHu28329
Clone ID Related Accession (Same CDS sequence)	NM_001193278.1 , XM_017029541.1
Accession Version	NM_001193278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF035360.1, AY539994.1 and AI371111.1. Transcript Variant: This variant (6) has an additional in-frame exon in the CDS and also lacks several 3' exons but has an alternate 3' segment, as compared to variant 1. The resulting isoform (3) has an additional segment in the middle region and has a shorter and different C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY539994.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
GAGTATCTCA TGGCCTATTT ATTTCTGTCT TTGGTGGCTT TCTTCAGATT GATTGCATTC 
CTGAATTTAA ACTTCAAAGG AAGACAAGGA AGGAAAGAAC ACACAATATT TATTGACTTG 
TCTTTCTTGA ACAAAAAAGT GATAGCACTC ACTTGTACTA CATTAGTAAA AACAGCTGGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180207.1
CDS	57..1679
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSEYLMAYLFLSLVAFFRLIAFLNLNFKGRQGRKEHTIFIDLSFLNKKVIALTCTTLVKTAG

Target ORF information:

RefSeq Version	NM_001193278.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193278.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
GAGTATCTCA TGGCCTATTT ATTTCTGTCT TTGGTGGCTT TCTTCAGATT GATTGCATTC 
CTGAATTTAA ACTTCAAAGG AAGACAAGGA AGGAAAGAAC ACACAATATT TATTGACTTG 
TCTTTCTTGA ACAAAAAAGT GATAGCACTC ACTTGTACTA CATTAGTAAA AACAGCTGGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_001193280.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180209.1 Homo sapiens midline 1 (MID1), transcript variant 8, mRNA.

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CloneID	OHu28335
Clone ID Related Accession (Same CDS sequence)	NM_001193280.1
Accession Version	NM_001193280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF035360.1, AY540023.1 and AI371111.1. Transcript Variant: This variant (8) has an alternate splice site in the CDS and also lacks several 3' exons but has an alternate 3' segment, as compared to variant 1. The resulting isoform (5) lacks an internal segment in the N-terminal region and has a shorter and different C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY540023.1, BF204562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTGAGTATC TCATGGCCTA TTTATTTCTG 
TCTTTGGTGG CTTTCTTCAG ATTGATTGCA TTCCTGAATT TAAACTTCAA AGGAAGACAA 
GGAAGGAAAG AACACACAAT ATTTATTGAC TTGTCTTTCT TGAACAAAAA AGTGATAGCA 
CTCACTTGTA CTACATTAGT AAAAACAGCT GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180209.1
CDS	57..1412
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSEYLMAYLFLSLVAFFRLIAFLNLNFKGRQGRKEHTIFIDLSFLNKKVIALTCTTLVKTAG

Target ORF information:

RefSeq Version	NM_001193280.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193280.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTGAGTATC TCATGGCCTA TTTATTTCTG 
TCTTTGGTGG CTTTCTTCAG ATTGATTGCA TTCCTGAATT TAAACTTCAA AGGAAGACAA 
GGAAGGAAAG AACACACAAT ATTTATTGAC TTGTCTTTCT TGAACAAAAA AGTGATAGCA 
CTCACTTGTA CTACATTAGT AAAAACAGCT GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_001193279.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180208.1 Homo sapiens midline 1 (MID1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu28337
Clone ID Related Accession (Same CDS sequence)	NM_001193279.1 , XM_017029542.1
Accession Version	NM_001193279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF035360.1, AY540022.1 and AI371111.1. Transcript Variant: This variant (7) lacks several 3' exons but has an alternate 3' segment, as compared to variant 1. The resulting isoform (4) has a shorter and different C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY540022.1, SRR1660805.312163.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTGAG TATCTCATGG CCTATTTATT TCTGTCTTTG 
GTGGCTTTCT TCAGATTGAT TGCATTCCTG AATTTAAACT TCAAAGGAAG ACAAGGAAGG 
AAAGAACACA CAATATTTAT TGACTTGTCT TTCTTGAACA AAAAAGTGAT AGCACTCACT 
TGTACTACAT TAGTAAAAAC AGCTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180208.1
CDS	57..1526
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSEYLMAYLFLSLVAFFRLIAFLNLNFKGRQGRKEHTIFIDLSFLNKKVIALTCTTLVKTAG

Target ORF information:

RefSeq Version	NM_001193279.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193279.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTGAG TATCTCATGG CCTATTTATT TCTGTCTTTG 
GTGGCTTTCT TCAGATTGAT TGCATTCCTG AATTTAAACT TCAAAGGAAG ACAAGGAAGG 
AAAGAACACA CAATATTTAT TGACTTGTCT TTCTTGAACA AAAAAGTGAT AGCACTCACT 
TGTACTACAT TAGTAAAAAC AGCTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_033289.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_150631.1 Homo sapiens midline 1 (MID1), transcript variant 5, mRNA.

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CloneID	OHu28338
Clone ID Related Accession (Same CDS sequence)	NM_033289.1 , NM_033289.2 , XM_017029537.1 , XM_017029538.1 , XM_017029539.1
Accession Version	NM_033289.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC309977.1, AK226104.1 and EF217426.1. Transcript Variant: This variant (5) has an alternate splice site in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal segment in the N-terminal region, as compared to the isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.222902.1, SRR1803616.44620.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_150631.1
CDS	402..2291
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_033289.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033289.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_033289.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_150631.1 Homo sapiens midline 1 (MID1), transcript variant 5, mRNA.

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CloneID	OHu28338
Clone ID Related Accession (Same CDS sequence)	NM_033289.1 , NM_033289.2 , XM_017029537.1 , XM_017029538.1 , XM_017029539.1
Accession Version	NM_033289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC309977.1, AK226104.1 and EF217426.1. On Jun 2, 2019 this sequence version replaced NM_033289.1. Transcript Variant: This variant (5) has an alternate splice site in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal segment in the N-terminal region, as compared to the isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.44620.1, SRR1803611.138190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_150631.1
CDS	353..2242
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	NM_033289.2
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033289.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( NM_001193281.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180210.1 Homo sapiens midline 1 (MID1), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu28339
Clone ID Related Accession (Same CDS sequence)	NM_001193281.1
Accession Version	NM_001193281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF035360.1 and AY539988.1. Transcript Variant: This variant (9) has an alternate splice site in the CDS and also lacks multiple 3' exons but has an alternate 3' exon, as compared to variant 1. The resulting isoform (6) lacks an internal segment in the N-terminal region and has a shorter and different C-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY539988.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AACTGGAATG TTCTGTACTG 
TTTCCCAGAG TTGCCCAATG GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180210.1
CDS	57..743
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVELECSVLFPRVAQWG

Target ORF information:

RefSeq Version	NM_001193281.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193281.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AACTGGAATG TTCTGTACTG 
TTTCCCAGAG TTGCCCAATG GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_017029541.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885030.1 Homo sapiens midline 1 (MID1), transcript variant X14, mRNA.

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CloneID	OHu28329
Clone ID Related Accession (Same CDS sequence)	NM_001193278.1 , XM_017029541.1
Accession Version	XM_017029541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
GAGTATCTCA TGGCCTATTT ATTTCTGTCT TTGGTGGCTT TCTTCAGATT GATTGCATTC 
CTGAATTTAA ACTTCAAAGG AAGACAAGGA AGGAAAGAAC ACACAATATT TATTGACTTG 
TCTTTCTTGA ACAAAAAAGT GATAGCACTC ACTTGTACTA CATTAGTAAA AACAGCTGGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885030.1
CDS	515..2137
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSEYLMAYLFLSLVAFFRLIAFLNLNFKGRQGRKEHTIFIDLSFLNKKVIALTCTTLVKTAG

Target ORF information:

RefSeq Version	XM_017029541.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029541.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
GAGTATCTCA TGGCCTATTT ATTTCTGTCT TTGGTGGCTT TCTTCAGATT GATTGCATTC 
CTGAATTTAA ACTTCAAAGG AAGACAAGGA AGGAAAGAAC ACACAATATT TATTGACTTG 
TCTTTCTTGA ACAAAAAAGT GATAGCACTC ACTTGTACTA CATTAGTAAA AACAGCTGGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_017029542.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885031.1 Homo sapiens midline 1 (MID1), transcript variant X15, mRNA.

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CloneID	OHu28337
Clone ID Related Accession (Same CDS sequence)	NM_001193279.1 , XM_017029542.1
Accession Version	XM_017029542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTGAG TATCTCATGG CCTATTTATT TCTGTCTTTG 
GTGGCTTTCT TCAGATTGAT TGCATTCCTG AATTTAAACT TCAAAGGAAG ACAAGGAAGG 
AAAGAACACA CAATATTTAT TGACTTGTCT TTCTTGAACA AAAAAGTGAT AGCACTCACT 
TGTACTACAT TAGTAAAAAC AGCTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885031.1
CDS	515..1984
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSEYLMAYLFLSLVAFFRLIAFLNLNFKGRQGRKEHTIFIDLSFLNKKVIALTCTTLVKTAG

Target ORF information:

RefSeq Version	XM_017029542.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029542.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTGAG TATCTCATGG CCTATTTATT TCTGTCTTTG 
GTGGCTTTCT TCAGATTGAT TGCATTCCTG AATTTAAACT TCAAAGGAAG ACAAGGAAGG 
AAAGAACACA CAATATTTAT TGACTTGTCT TTCTTGAACA AAAAAGTGAT AGCACTCACT 
TGTACTACAT TAGTAAAAAC AGCTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_017029537.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885026.1 Homo sapiens midline 1 (MID1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu28338
Clone ID Related Accession (Same CDS sequence)	NM_033289.1 , NM_033289.2 , XM_017029537.1 , XM_017029538.1 , XM_017029539.1
Accession Version	XM_017029537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885026.1
CDS	515..2404
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_017029537.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029537.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_017029538.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885027.1 Homo sapiens midline 1 (MID1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu28338
Clone ID Related Accession (Same CDS sequence)	NM_033289.1 , NM_033289.2 , XM_017029537.1 , XM_017029538.1 , XM_017029539.1
Accession Version	XM_017029538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885027.1
CDS	214..2103
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_017029538.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029538.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_017029539.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885028.1 Homo sapiens midline 1 (MID1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu28338
Clone ID Related Accession (Same CDS sequence)	NM_033289.1 , NM_033289.2 , XM_017029537.1 , XM_017029538.1 , XM_017029539.1
Accession Version	XM_017029539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885028.1
CDS	376..2265
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_017029539.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029539.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAGTCAATGC ATCACGTCAA 
GAAGCCAAAT TGACAGAGGA GTGTGATCTT CTCATTGAGA TCATTCAGCA AAGACGACAG 
ATTATTGGAA CCAAGATCAA AGAAGGGAAG GTGATGAGGC TTCGCAAACT GGCTCAGCAG 
ATTGCAAACT GCAAACAGTG CATTGAGCGG TCAGCATCAC TCATCTCCCA AGCGGAACAC 
TCTCTGAAGG AGAATGATCA TGCGCGTTTC CTACAGACTG CTAAGAATAT CACCGAGAGA 
GTCTCCATGG CAACTGCATC CTCCCAGGTT CTAATTCCTG AAATCAACCT CAATGACACA 
TTTGACACCT TTGCCTTAGA TTTTTCCCGA GAGAAGAAAC TGCTAGAATG TCTGGATTAC 
CTTACAGCTC CCAACCCTCC CACAATTAGA GAAGAGCTCT GCACAGCTTC ATATGACACC 
ATCACTGTGC ATTGGACCTC CGATGATGAG TTCAGCGTGG TCTCCTACGA GCTCCAGTAC 
ACCATATTCA CCGGACAAGC CAACGTCGTT AGTCTGTGTA ATTCGGCTGA TAGCTGGATG 
ATAGTACCCA ACATCAAGCA GAACCACTAC ACGGTGCACG GTCTGCAGAG CGGCACCAAG 
TACATCTTCA TGGTCAAGGC CATCAACCAG GCGGGCAGCC GCAGCAGTGA GCCTGGGAAG 
TTGAAGACAA ACAGCCAACC ATTTAAACTG GATCCCAAAT CTGCTCATCG AAAACTGAAG 
GTGTCCCATG ATAACTTGAC AGTAGAACGT GATGAGTCAT CATCCAAGAA GAGTCACACA 
CCTGAACGCT TCACCAGCCA GGGGAGCTAT GGAGTAGCTG GAAATGTGTT TATTGATAGT 
GGCCGGCATT ATTGGGAAGT GGTCATAAGT GGAAGCACAT GGTATGCCAT TGGTCTTGCT 
TACAAATCAG CCCCGAAGCA TGAATGGATT GGGAAGAACT CTGCTTCCTG GGCGCTCTGC 
CGCTGCAACA ATAACTGGGT GGTGAGACAC AATAGCAAGG AAATCCCCAT TGAGCCTGCC 
CCCCACCTCC GGCGCGTGGG CATCCTGCTG GACTATGATA ACGGCTCTAT CGCCTTTTAT 
GATGCTTTGA ACTCCATCCA CCTCTACACC TTCGACGTCG CATTTGCGCA GCCTGTTTGC 
CCCACCTTCA CCGTGTGGAA CAAGTGTCTG ACGATTATCA CTGGGCTCCC TATCCCAGAC 
CATTTGGACT GCACAGAGCA GCTGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_017029540.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885029.1 Homo sapiens midline 1 (MID1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu99314
Clone ID Related Accession (Same CDS sequence)	XM_017029540.1
Accession Version	XM_017029540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885029.1
CDS	515..2173
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTW

Target ORF information:

RefSeq Version	XM_017029540.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029540.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAGTCA ATGCATCACG TCAAGAAGCC 
AAATTGACAG AGGAGTGTGA TCTTCTCATT GAGATCATTC AGCAAAGACG ACAGATTATT 
GGAACCAAGA TCAAAGAAGG GAAGGTGATG AGGCTTCGCA AACTGGCTCA GCAGATTGCA 
AACTGCAAAC AGTGCATTGA GCGGTCAGCA TCACTCATCT CCCAAGCGGA ACACTCTCTG 
AAGGAGAATG ATCATGCGCG TTTCCTACAG ACTGCTAAGA ATATCACCGA GAGAGTCTCC 
ATGGCAACTG CATCCTCCCA GGTTCTAATT CCTGAAATCA ACCTCAATGA CACATTTGAC 
ACCTTTGCCT TAGATTTTTC CCGAGAGAAG AAACTGCTAG AATGTCTGGA TTACCTTACA 
GCTCCCAACC CTCCCACAAT TAGAGAAGAG CTCTGCACAG CTTCATATGA CACCATCACT 
GTGCATTGGA CCTCCGATGA TGAGTTCAGC GTGGTCTCCT ACGAGCTCCA GTACACCATA 
TTCACCGGAC AAGCCAACGT CGTTAGTCTG TGTAATTCGG CTGATAGCTG GATGATAGTA 
CCCAACATCA AGCAGAACCA CTACACGGTG CACGGTCTGC AGAGCGGCAC CAAGTACATC 
TTCATGGTCA AGGCCATCAA CCAGGCGGGC AGCCGCAGCA GTGAGCCTGG GAAGTTGAAG 
ACAAACAGCC AACCATTTAA ACTGGATCCC AAATCTGCTC ATCGAAAACT GAAGGTGTCC 
CATGATAACT TGACAGTAGA ACGTGATGAG TCATCATCCA AGAAGAGTCA CACACCTGAA 
CGCTTCACCA GCCAGGGGAG CTATGGAGTA GCTGGAAATG TGTTTATTGA TAGTGGCCGG 
CATTATTGGG AAGTGGTCAT AAGTGGAAGC ACATGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005274537.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274594.1 Homo sapiens midline 1 (MID1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu53923
Clone ID Related Accession (Same CDS sequence)	XM_005274537.1 , XM_017029534.1 , XM_006724492.3 , XM_011545525.2 , XM_011545526.2 , XM_011545527.2 , XM_005274536.1
Accession Version	XM_005274537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274594.1
CDS	191..2347
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005274537.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274537.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_017029534.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885023.1 Homo sapiens midline 1 (MID1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu53923
Clone ID Related Accession (Same CDS sequence)	XM_005274537.1 , XM_017029534.1 , XM_006724492.3 , XM_011545525.2 , XM_011545526.2 , XM_011545527.2 , XM_005274536.1
Accession Version	XM_017029534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885023.1
CDS	380..2536
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_017029534.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029534.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_006724492.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006724555.1 Homo sapiens midline 1 (MID1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu53923
Clone ID Related Accession (Same CDS sequence)	XM_005274537.1 , XM_017029534.1 , XM_006724492.3 , XM_011545525.2 , XM_011545526.2 , XM_011545527.2 , XM_005274536.1
Accession Version	XM_006724492.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006724492.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006724555.1
CDS	813..2969
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_006724492.3
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006724492.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_011545525.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543827.1 Homo sapiens midline 1 (MID1), transcript variant X1, mRNA.

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CloneID	OHu53923
Clone ID Related Accession (Same CDS sequence)	XM_005274537.1 , XM_017029534.1 , XM_006724492.3 , XM_011545525.2 , XM_011545526.2 , XM_011545527.2 , XM_005274536.1
Accession Version	XM_011545525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545525.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543827.1
CDS	515..2671
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_011545525.2
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545525.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_011545526.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543828.1 Homo sapiens midline 1 (MID1), transcript variant X3, mRNA.

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CloneID	OHu53923
Clone ID Related Accession (Same CDS sequence)	XM_005274537.1 , XM_017029534.1 , XM_006724492.3 , XM_011545525.2 , XM_011545526.2 , XM_011545527.2 , XM_005274536.1
Accession Version	XM_011545526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543828.1
CDS	550..2706
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_011545526.2
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545526.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_011545527.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543829.1 Homo sapiens midline 1 (MID1), transcript variant X6, mRNA.

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CloneID	OHu53923
Clone ID Related Accession (Same CDS sequence)	XM_005274537.1 , XM_017029534.1 , XM_006724492.3 , XM_011545525.2 , XM_011545526.2 , XM_011545527.2 , XM_005274536.1
Accession Version	XM_011545527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543829.1
CDS	460..2616
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_011545527.2
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545527.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_005274536.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005274593.1 Homo sapiens midline 1 (MID1), transcript variant X4, mRNA.

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CloneID	OHu53923
Clone ID Related Accession (Same CDS sequence)	XM_005274537.1 , XM_017029534.1 , XM_006724492.3 , XM_011545525.2 , XM_011545526.2 , XM_011545527.2 , XM_005274536.1
Accession Version	XM_005274536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005274593.1
CDS	214..2370
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_005274536.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005274536.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGGTGA ATATGTACTG TGTGACCGAT GACCAGTTAA TCTGTGCCTT GTGTAAACTG 
GTTGGGCGGC ACCGCGATCA TCAGGTGGCA GCTTTGAGTG AGCGCTATGA CAAATTGAAG 
CAAAACTTAG AGAGTAACCT CACCAACCTT ATTAAGAGGA ACACAGAACT GGAGACCCTT 
TTGGCTAAAC TCATCCAAAC CTGTCAACAT GTTGAAACTG TAAAGGATCA GAAAGTCATT 
GGCAGAGAGA AGCAGCAGTA TTACCCTAGG AATAGGAGAA AGTGGGTCAG CAACTCCCTA 
CAGGCAAGAC TGATGGGGAG AAGATGCATT TCAAGACGCG AAAGAAAACT GACTCTAGAT 
GTAGAGAAGG TCAATGCATC ACGTCAAGAA GCCAAATTGA CAGAGGAGTG TGATCTTCTC 
ATTGAGATCA TTCAGCAAAG ACGACAGATT ATTGGAACCA AGATCAAAGA AGGGAAGGTG 
ATGAGGCTTC GCAAACTGGC TCAGCAGATT GCAAACTGCA AACAGTGCAT TGAGCGGTCA 
GCATCACTCA TCTCCCAAGC GGAACACTCT CTGAAGGAGA ATGATCATGC GCGTTTCCTA 
CAGACTGCTA AGAATATCAC CGAGAGAGTC TCCATGGCAA CTGCATCCTC CCAGGTTCTA 
ATTCCTGAAA TCAACCTCAA TGACACATTT GACACCTTTG CCTTAGATTT TTCCCGAGAG 
AAGAAACTGC TAGAATGTCT GGATTACCTT ACAGCTCCCA ACCCTCCCAC AATTAGAGAA 
GAGCTCTGCA CAGCTTCATA TGACACCATC ACTGTGCATT GGACCTCCGA TGATGAGTTC 
AGCGTGGTCT CCTACGAGCT CCAGTACACC ATATTCACCG GACAAGCCAA CGTCGTTAGT 
CTGTGTAATT CGGCTGATAG CTGGATGATA GTACCCAACA TCAAGCAGAA CCACTACACG 
GTGCACGGTC TGCAGAGCGG CACCAAGTAC ATCTTCATGG TCAAGGCCAT CAACCAGGCG 
GGCAGCCGCA GCAGTGAGCC TGGGAAGTTG AAGACAAACA GCCAACCATT TAAACTGGAT 
CCCAAATCTG CTCATCGAAA ACTGAAGGTG TCCCATGATA ACTTGACAGT AGAACGTGAT 
GAGTCATCAT CCAAGAAGAG TCACACACCT GAACGCTTCA CCAGCCAGGG GAGCTATGGA 
GTAGCTGGAA ATGTGTTTAT TGATAGTGGC CGGCATTATT GGGAAGTGGT CATAAGTGGA 
AGCACATGGT ATGCCATTGG TCTTGCTTAC AAATCAGCCC CGAAGCATGA ATGGATTGGG 
AAGAACTCTG CTTCCTGGGC GCTCTGCCGC TGCAACAATA ACTGGGTGGT GAGACACAAT 
AGCAAGGAAA TCCCCATTGA GCCTGCCCCC CACCTCCGGC GCGTGGGCAT CCTGCTGGAC 
TATGATAACG GCTCTATCGC CTTTTATGAT GCTTTGAACT CCATCCACCT CTACACCTTC 
GACGTCGCAT TTGCGCAGCC TGTTTGCCCC ACCTTCACCG TGTGGAACAA GTGTCTGACG 
ATTATCACTG GGCTCCCTAT CCCAGACCAT TTGGACTGCA CAGAGCAGCT GCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID1 ( XM_017029535.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885024.1 Homo sapiens midline 1 (MID1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu53924
Clone ID Related Accession (Same CDS sequence)	XM_017029535.1
Accession Version	XM_017029535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAACTGTAAA GGATCAGAAA 
GTCATTGGCA GAGAGAAGCA GCAGTATTAC CCTAGGAATA GGAGAAAGTG GGTCAGCAAC 
TCCCTACAGG CAAGACTGAT GGGGAGAAGA TGCATTTCAA GACGCGAAAG AAAACTGACT 
CTAGATGTAG AGAAGGTCAA TGCATCACGT CAAGAAGCCA AATTGACAGA GGAGTGTGAT 
CTTCTCATTG AGATCATTCA GCAAAGACGA CAGATTATTG GAACCAAGAT CAAAGAAGGG 
AAGGTGATGA GGCTTCGCAA ACTGGCTCAG CAGATTGCAA ACTGCAAACA GTGCATTGAG 
CGGTCAGCAT CACTCATCTC CCAAGCGGAA CACTCTCTGA AGGAGAATGA TCATGCGCGT 
TTCCTACAGA CTGCTAAGAA TATCACCGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
GTTCTAATTC CTGAAATCAA CCTCAATGAC ACATTTGACA CCTTTGCCTT AGATTTTTCC 
CGAGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTTACAG CTCCCAACCC TCCCACAATT 
AGAGAAGAGC TCTGCACAGC TTCATATGAC ACCATCACTG TGCATTGGAC CTCCGATGAT 
GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAACGTC 
GTTAGTCTGT GTAATTCGGC TGATAGCTGG ATGATAGTAC CCAACATCAA GCAGAACCAC 
TACACGGTGC ACGGTCTGCA GAGCGGCACC AAGTACATCT TCATGGTCAA GGCCATCAAC 
CAGGCGGGCA GCCGCAGCAG TGAGCCTGGG AAGTTGAAGA CAAACAGCCA ACCATTTAAA 
CTGGATCCCA AATCTGCTCA TCGAAAACTG AAGGTGTCCC ATGATAACTT GACAGTAGAA 
CGTGATGAGT CATCATCCAA GAAGAGTCAC ACACCTGAAC GCTTCACCAG CCAGGGGAGC 
TATGGAGTAG CTGGAAATGT GTTTATTGAT AGTGGCCGGC ATTATTGGGA AGTGGTCATA 
AGTGGAAGCA CATGGTATGC CATTGGTCTT GCTTACAAAT CAGCCCCGAA GCATGAATGG 
ATTGGGAAGA ACTCTGCTTC CTGGGCGCTC TGCCGCTGCA ACAATAACTG GGTGGTGAGA 
CACAATAGCA AGGAAATCCC CATTGAGCCT GCCCCCCACC TCCGGCGCGT GGGCATCCTG 
CTGGACTATG ATAACGGCTC TATCGCCTTT TATGATGCTT TGAACTCCAT CCACCTCTAC 
ACCTTCGACG TCGCATTTGC GCAGCCTGTT TGCCCCACCT TCACCGTGTG GAACAAGTGT 
CTGACGATTA TCACTGGGCT CCCTATCCCA GACCATTTGG ACTGCACAGA GCAGCTGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885024.1
CDS	515..2557
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKQNLESNLTNLIKRNTELETLLAKLIQTCQHVETVKDQKVIGREKQQYYPRNRRKWVSNSLQARLMGRRCISRRERKLTLDVEKVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Target ORF information:

RefSeq Version	XM_017029535.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 1 (MID1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029535.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGC CCTATTTGTC TGGAGCTCTT TGAGGACCCT 
CTTCTACTGC CCTGCGCACA CAGCCTCTGC TTCAACTGCG CCCACCGCAT CCTAGTATCA 
CACTGTGCCA CCAACGAGTC TGTGGAGTCC ATCACCGCCT TCCAGTGCCC CACCTGCCGG 
CATGTCATCA CCCTCAGCCA GCGAGGTCTA GACGGGCTCA AGCGCAACGT CACCCTACAG 
AACATCATCG ACAGGTTCCA GAAAGCATCA GTGAGCGGGC CCAACTCTCC CAGCGAGACC 
CGTCGGGAGC GGGCCTTTGA CGCCAACACC ATGACCTCCG CCGAGAAGGT CCTCTGCCAG 
TTTTGTGACC AGGATCCTGC CCAGGACGCT GTGAAGACCT GTGTCACTTG TGAAGTATCC 
TACTGTGACG AGTGCCTGAA AGCCACTCAC CCGAATAAGA AGCCCTTTAC AGGCCATCGT 
CTGATTGAGC CAATTCCGGA CTCTCACATC CGGGGGCTGA TGTGCTTGGA GCATGAGGAT 
GAGAAGCAAA ACTTAGAGAG TAACCTCACC AACCTTATTA AGAGGAACAC AGAACTGGAG 
ACCCTTTTGG CTAAACTCAT CCAAACCTGT CAACATGTTG AAACTGTAAA GGATCAGAAA 
GTCATTGGCA GAGAGAAGCA GCAGTATTAC CCTAGGAATA GGAGAAAGTG GGTCAGCAAC 
TCCCTACAGG CAAGACTGAT GGGGAGAAGA TGCATTTCAA GACGCGAAAG AAAACTGACT 
CTAGATGTAG AGAAGGTCAA TGCATCACGT CAAGAAGCCA AATTGACAGA GGAGTGTGAT 
CTTCTCATTG AGATCATTCA GCAAAGACGA CAGATTATTG GAACCAAGAT CAAAGAAGGG 
AAGGTGATGA GGCTTCGCAA ACTGGCTCAG CAGATTGCAA ACTGCAAACA GTGCATTGAG 
CGGTCAGCAT CACTCATCTC CCAAGCGGAA CACTCTCTGA AGGAGAATGA TCATGCGCGT 
TTCCTACAGA CTGCTAAGAA TATCACCGAG AGAGTCTCCA TGGCAACTGC ATCCTCCCAG 
GTTCTAATTC CTGAAATCAA CCTCAATGAC ACATTTGACA CCTTTGCCTT AGATTTTTCC 
CGAGAGAAGA AACTGCTAGA ATGTCTGGAT TACCTTACAG CTCCCAACCC TCCCACAATT 
AGAGAAGAGC TCTGCACAGC TTCATATGAC ACCATCACTG TGCATTGGAC CTCCGATGAT 
GAGTTCAGCG TGGTCTCCTA CGAGCTCCAG TACACCATAT TCACCGGACA AGCCAACGTC 
GTTAGTCTGT GTAATTCGGC TGATAGCTGG ATGATAGTAC CCAACATCAA GCAGAACCAC 
TACACGGTGC ACGGTCTGCA GAGCGGCACC AAGTACATCT TCATGGTCAA GGCCATCAAC 
CAGGCGGGCA GCCGCAGCAG TGAGCCTGGG AAGTTGAAGA CAAACAGCCA ACCATTTAAA 
CTGGATCCCA AATCTGCTCA TCGAAAACTG AAGGTGTCCC ATGATAACTT GACAGTAGAA 
CGTGATGAGT CATCATCCAA GAAGAGTCAC ACACCTGAAC GCTTCACCAG CCAGGGGAGC 
TATGGAGTAG CTGGAAATGT GTTTATTGAT AGTGGCCGGC ATTATTGGGA AGTGGTCATA 
AGTGGAAGCA CATGGTATGC CATTGGTCTT GCTTACAAAT CAGCCCCGAA GCATGAATGG 
ATTGGGAAGA ACTCTGCTTC CTGGGCGCTC TGCCGCTGCA ACAATAACTG GGTGGTGAGA 
CACAATAGCA AGGAAATCCC CATTGAGCCT GCCCCCCACC TCCGGCGCGT GGGCATCCTG 
CTGGACTATG ATAACGGCTC TATCGCCTTT TATGATGCTT TGAACTCCAT CCACCTCTAC 
ACCTTCGACG TCGCATTTGC GCAGCCTGTT TGCCCCACCT TCACCGTGTG GAACAAGTGT 
CTGACGATTA TCACTGGGCT CCCTATCCCA GACCATTTGG ACTGCACAGA GCAGCTGCCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNAs miR-19, miR-340, miR-374 and miR-542 regulate MID1 protein expression.
PloS one13(1)e0190437(2018)
Unterbruner K,Matthes F,Schilling J,Nalavade R,Weber S,Winter J,Krau? S

The E3 ubiquitin ligase MID1/TRIM18 promotes atypical ubiquitination of the BRCA2-associated factor 35, BRAF35.
Biochimica et biophysica acta1864(10)1844-1854(2017 10)
Zanchetta ME,Napolitano LMR,Maddalo D,Meroni G

Structural and functional observations of the P151L MID1 mutation reveal alpha4 plays a significant role in X-linked Opitz Syndrome.
The FEBS journal284(14)2183-2193(2017 Jul)
Wright KM,Du H,Massiah MA

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MID1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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