ASAH1 gene cDNA ORF clone, Homo sapiens(Human)

mRNA	Protein	Name
NM_004315.5	NP_004306.3	acid ceramidase isoform b
NM_001127505.2	NP_001120977.1	acid ceramidase isoform c
NM_177924.5	NP_808592.2	acid ceramidase isoform a preproprotein
NM_001127505.3	NP_001120977.1	acid ceramidase isoform c
NM_004315.6	NP_004306.3	acid ceramidase isoform b
NM_001363743.1	NP_001350672.1	acid ceramidase isoform d
NM_177924.4	NP_808592.2	acid ceramidase isoform a preproprotein
NM_001363743.2	NP_001350672.1	acid ceramidase isoform d
XM_005273504.3	XP_005273561.1	acid ceramidase isoform X1
NM_177924.3	NP_808592.2	acid ceramidase isoform a preproprotein

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20258	NM_004315.6 Latest version!	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20264	NM_177924.5 Latest version!	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20041	NM_001127505.3 Latest version!	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$230.30 $329.00
OHu42065	XM_005273504.3 Latest version!	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu107073	NM_001363743.2 Latest version!	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu20041	NM_001127505.2	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$230.30 $329.00
OHu20258	NM_004315.5	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu20264	NM_177924.4	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu107073	NM_001363743.1	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu20264	NM_177924.3	Homo sapiens N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

ASAH1 ( NM_004315.6 ) cDNA ORF clone, Homo sapiens(Human) -> NP_004306.3 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20258
Clone ID Related Accession (Same CDS sequence)	NM_004315.5 , NM_004315.6
Accession Version	NM_004315.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	acid ceramidase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA260852.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1, AI241768.1 and AC124242.6. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_004315.5. Transcript Variant: This variant (2) encodes the longest isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.110388.1, SRR1660803.78057.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA 
GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACGTACAGA 
GGTGCAGTTC CATGGTACAC CATAAATCTT GACTTACCAC CCTACAAAAG ATGGCATGAA 
TTGATGCTTG ACAAGGCACC AGTGCTAAAG GTTATAGTGA ATTCTCTGAA GAATATGATA 
AATACATTCG TGCCAAGTGG AAAAATTATG CAGGTGGTGG ATGAAAAATT GCCTGGCCTA 
CTTGGCAACT TTCCTGGCCC TTTTGAAGAG GAAATGAAGG GTATTGCCGC TGTTACTGAT 
ATACCTTTAG GAGAGATTAT TTCATTCAAT ATTTTTTATG AATTATTTAC CATTTGTACT 
TCAATAGTAG CAGAAGACAA AAAAGGTCAT CTAATACATG GGAGAAACAT GGATTTTGGA 
GTATTTCTTG GGTGGAACAT AAATAATGAT ACCTGGGTCA TAACTGAGCA ACTAAAACCT 
TTAACAGTGA ATTTGGATTT CCAAAGAAAC AACAAAACTG TCTTCAAGGC TTCAAGCTTT 
GCTGGCTATG TGGGCATGTT AACAGGATTC AAACCAGGAC TGTTCAGTCT TACACTGAAT 
GAACGTTTCA GTATAAATGG TGGTTATCTG GGTATTCTAG AATGGATTCT GGGAAAGAAA 
GATGTCATGT GGATAGGGTT CCTCACTAGA ACAGTTCTGG AAAATAGCAC AAGTTATGAA 
GAAGCCAAGA ATTTATTGAC CAAGACCAAG ATATTGGCCC CAGCCTACTT TATCCTGGGA 
GGCAACCAGT CTGGGGAAGG TTGTGTGATT ACACGAGACA GAAAGGAATC ATTGGATGTA 
TATGAACTCG ATGCTAAGCA GGGTAGATGG TATGTGGTAC AAACAAATTA TGACCGTTGG 
AAACATCCCT TCTTCCTTGA TGATCGCAGA ACGCCTGCAA AGATGTGTCT GAACCGCACC 
AGCCAAGAGA ATATCTCATT TGAAACCATG TATGATGTCC TGTCAACAAA ACCTGTCCTC 
AACAAGCTGA CCGTATACAC AACCTTGATA GATGTTACCA AAGGTCAATT CGAAACTTAC 
CTGCGGGACT GCCCTGACCC TTGTATAGGT TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004306.3
CDS	161..1396
Translation	MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_004315.6
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004315.6

ORF Insert Sequence:

ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA 
GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACGTACAGA 
GGTGCAGTTC CATGGTACAC CATAAATCTT GACTTACCAC CCTACAAAAG ATGGCATGAA 
TTGATGCTTG ACAAGGCACC AGTGCTAAAG GTTATAGTGA ATTCTCTGAA GAATATGATA 
AATACATTCG TGCCAAGTGG AAAAATTATG CAGGTGGTGG ATGAAAAATT GCCTGGCCTA 
CTTGGCAACT TTCCTGGCCC TTTTGAAGAG GAAATGAAGG GTATTGCCGC TGTTACTGAT 
ATACCTTTAG GAGAGATTAT TTCATTCAAT ATTTTTTATG AATTATTTAC CATTTGTACT 
TCAATAGTAG CAGAAGACAA AAAAGGTCAT CTAATACATG GGAGAAACAT GGATTTTGGA 
GTATTTCTTG GGTGGAACAT AAATAATGAT ACCTGGGTCA TAACTGAGCA ACTAAAACCT 
TTAACAGTGA ATTTGGATTT CCAAAGAAAC AACAAAACTG TCTTCAAGGC TTCAAGCTTT 
GCTGGCTATG TGGGCATGTT AACAGGATTC AAACCAGGAC TGTTCAGTCT TACACTGAAT 
GAACGTTTCA GTATAAATGG TGGTTATCTG GGTATTCTAG AATGGATTCT GGGAAAGAAA 
GATGTCATGT GGATAGGGTT CCTCACTAGA ACAGTTCTGG AAAATAGCAC AAGTTATGAA 
GAAGCCAAGA ATTTATTGAC CAAGACCAAG ATATTGGCCC CAGCCTACTT TATCCTGGGA 
GGCAACCAGT CTGGGGAAGG TTGTGTGATT ACACGAGACA GAAAGGAATC ATTGGATGTA 
TATGAACTCG ATGCTAAGCA GGGTAGATGG TATGTGGTAC AAACAAATTA TGACCGTTGG 
AAACATCCCT TCTTCCTTGA TGATCGCAGA ACGCCTGCAA AGATGTGTCT GAACCGCACC 
AGCCAAGAGA ATATCTCATT TGAAACCATG TATGATGTCC TGTCAACAAA ACCTGTCCTC 
AACAAGCTGA CCGTATACAC AACCTTGATA GATGTTACCA AAGGTCAATT CGAAACTTAC 
CTGCGGGACT GCCCTGACCC TTGTATAGGT TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH1 ( NM_177924.5 ) cDNA ORF clone, Homo sapiens(Human) -> NP_808592.2 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20264
Clone ID Related Accession (Same CDS sequence)	NM_177924.5 , NM_177924.4 , NM_177924.3
Accession Version	NM_177924.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	acid ceramidase isoform a preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA306573.1, DA260852.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1, AI241768.1 and AC124242.6. On Nov 22, 2018 this sequence version replaced NM_177924.4. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 2. The resulting isoform (a) includes a predicted signal peptide, is shorter and has a distinct N-terminus compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.46311.1, SRR5189664.67191.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000637790.2/ ENSP00000490272.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA 
CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA 
AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG 
AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA 
TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC 
GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT 
ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC 
ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG 
CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG 
GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT 
CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT 
CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC 
ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC 
TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA 
TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT 
TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT 
CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA 
AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA 
TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808592.2
CDS	50..1237
Translation	MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_177924.5
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177924.5

ORF Insert Sequence:

ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA 
CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA 
AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG 
AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA 
TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC 
GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT 
ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC 
ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG 
CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG 
GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT 
CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT 
CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC 
ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC 
TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA 
TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT 
TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT 
CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA 
AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA 
TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH1 ( NM_177924.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_808592.2 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20264
Clone ID Related Accession (Same CDS sequence)	NM_177924.5 , NM_177924.4 , NM_177924.3
Accession Version	NM_177924.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	acid ceramidase isoform a preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA306573.1, DA260852.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1 and AI241768.1. On Nov 5, 2015 this sequence version replaced NM_177924.3. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 2. The resulting isoform (a) includes a predicted signal peptide, is shorter and has a distinct N-terminus compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.60368.1, SRR1660803.13604.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154125 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA 
CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA 
AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG 
AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA 
TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC 
GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT 
ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC 
ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG 
CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG 
GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT 
CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT 
CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC 
ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC 
TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA 
TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT 
TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT 
CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA 
AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA 
TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808592.2
CDS	313..1500
Translation	MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_177924.4
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177924.4

ORF Insert Sequence:

ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA 
CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA 
AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG 
AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA 
TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC 
GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT 
ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC 
ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG 
CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG 
GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT 
CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT 
CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC 
ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC 
TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA 
TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT 
TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT 
CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA 
AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA 
TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH1 ( NM_177924.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_808592.2 Homo sapiens N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20264
Clone ID Related Accession (Same CDS sequence)	NM_177924.5 , NM_177924.4 , NM_177924.3
Accession Version	NM_177924.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	acid ceramidase isoform a preproprotein
Comment

ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA 
CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA 
AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG 
AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA 
TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC 
GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT 
ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC 
ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG 
CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG 
GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT 
CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT 
CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC 
ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC 
TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA 
TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT 
TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT 
CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA 
AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA 
TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808592.2
Translation	MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_177924.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177924.3

ORF Insert Sequence:

ATGCCGGGCC GGAGTTGCGT CGCCTTAGTC CTCCTGGCTG CCGCCGTCAG CTGTGCCGTC 
GCGCAGCACG CGCCGCCGTG GACAGAGGAC TGCAGAAAAT CAACCTATCC TCCTTCAGGA 
CCAACGTACA GAGGTGCAGT TCCATGGTAC ACCATAAATC TTGACTTACC ACCCTACAAA 
AGATGGCATG AATTGATGCT TGACAAGGCA CCAGTGCTAA AGGTTATAGT GAATTCTCTG 
AAGAATATGA TAAATACATT CGTGCCAAGT GGAAAAATTA TGCAGGTGGT GGATGAAAAA 
TTGCCTGGCC TACTTGGCAA CTTTCCTGGC CCTTTTGAAG AGGAAATGAA GGGTATTGCC 
GCTGTTACTG ATATACCTTT AGGAGAGATT ATTTCATTCA ATATTTTTTA TGAATTATTT 
ACCATTTGTA CTTCAATAGT AGCAGAAGAC AAAAAAGGTC ATCTAATACA TGGGAGAAAC 
ATGGATTTTG GAGTATTTCT TGGGTGGAAC ATAAATAATG ATACCTGGGT CATAACTGAG 
CAACTAAAAC CTTTAACAGT GAATTTGGAT TTCCAAAGAA ACAACAAAAC TGTCTTCAAG 
GCTTCAAGCT TTGCTGGCTA TGTGGGCATG TTAACAGGAT TCAAACCAGG ACTGTTCAGT 
CTTACACTGA ATGAACGTTT CAGTATAAAT GGTGGTTATC TGGGTATTCT AGAATGGATT 
CTGGGAAAGA AAGATGTCAT GTGGATAGGG TTCCTCACTA GAACAGTTCT GGAAAATAGC 
ACAAGTTATG AAGAAGCCAA GAATTTATTG ACCAAGACCA AGATATTGGC CCCAGCCTAC 
TTTATCCTGG GAGGCAACCA GTCTGGGGAA GGTTGTGTGA TTACACGAGA CAGAAAGGAA 
TCATTGGATG TATATGAACT CGATGCTAAG CAGGGTAGAT GGTATGTGGT ACAAACAAAT 
TATGACCGTT GGAAACATCC CTTCTTCCTT GATGATCGCA GAACGCCTGC AAAGATGTGT 
CTGAACCGCA CCAGCCAAGA GAATATCTCA TTTGAAACCA TGTATGATGT CCTGTCAACA 
AAACCTGTCC TCAACAAGCT GACCGTATAC ACAACCTTGA TAGATGTTAC CAAAGGTCAA 
TTCGAAACTT ACCTGCGGGA CTGCCCTGAC CCTTGTATAG GTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH1 ( NM_001127505.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001120977.1 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA.

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CloneID	OHu20041
Clone ID Related Accession (Same CDS sequence)	NM_001127505.2 , NM_001127505.3
Accession Version	NM_001127505.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	acid ceramidase isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA260852.1, BC016828.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1 and AI241768.1. On Nov 5, 2015 this sequence version replaced NM_001127505.1. Transcript Variant: This variant (3) contains an alternate in-frame exon and lacks another alternate in-frame exon compared to variant 2. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016828.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA 
GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACTGTCTTC 
CCTGCTGTTA TAAGGTACAG AGGTGCAGTT CCATGGTACA CCATAAATCT TGACTTACCA 
CCCTACAAAA GATGGCATGA ATTGATGCTT GACAAGGCAC CAGTGCCTGG CCTACTTGGC 
AACTTTCCTG GCCCTTTTGA AGAGGAAATG AAGGGTATTG CCGCTGTTAC TGATATACCT 
TTAGGAGAGA TTATTTCATT CAATATTTTT TATGAATTAT TTACCATTTG TACTTCAATA 
GTAGCAGAAG ACAAAAAAGG TCATCTAATA CATGGGAGAA ACATGGATTT TGGAGTATTT 
CTTGGGTGGA ACATAAATAA TGATACCTGG GTCATAACTG AGCAACTAAA ACCTTTAACA 
GTGAATTTGG ATTTCCAAAG AAACAACAAA ACTGTCTTCA AGGCTTCAAG CTTTGCTGGC 
TATGTGGGCA TGTTAACAGG ATTCAAACCA GGACTGTTCA GTCTTACACT GAATGAACGT 
TTCAGTATAA ATGGTGGTTA TCTGGGTATT CTAGAATGGA TTCTGGGAAA GAAAGATGTC 
ATGTGGATAG GGTTCCTCAC TAGAACAGTT CTGGAAAATA GCACAAGTTA TGAAGAAGCC 
AAGAATTTAT TGACCAAGAC CAAGATATTG GCCCCAGCCT ACTTTATCCT GGGAGGCAAC 
CAGTCTGGGG AAGGTTGTGT GATTACACGA GACAGAAAGG AATCATTGGA TGTATATGAA 
CTCGATGCTA AGCAGGGTAG ATGGTATGTG GTACAAACAA ATTATGACCG TTGGAAACAT 
CCCTTCTTCC TTGATGATCG CAGAACGCCT GCAAAGATGT GTCTGAACCG CACCAGCCAA 
GAGAATATCT CATTTGAAAC CATGTATGAT GTCCTGTCAA CAAAACCTGT CCTCAACAAG 
CTGACCGTAT ACACAACCTT GATAGATGTT ACCAAAGGTC AATTCGAAAC TTACCTGCGG 
GACTGCCCTG ACCCTTGTAT AGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120977.1
CDS	198..1367
Translation	MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPTVFPAVIRYRGAVPWYTINLDLPPYKRWHELMLDKAPVPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_001127505.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127505.2

ORF Insert Sequence:

ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA 
GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACTGTCTTC 
CCTGCTGTTA TAAGGTACAG AGGTGCAGTT CCATGGTACA CCATAAATCT TGACTTACCA 
CCCTACAAAA GATGGCATGA ATTGATGCTT GACAAGGCAC CAGTGCCTGG CCTACTTGGC 
AACTTTCCTG GCCCTTTTGA AGAGGAAATG AAGGGTATTG CCGCTGTTAC TGATATACCT 
TTAGGAGAGA TTATTTCATT CAATATTTTT TATGAATTAT TTACCATTTG TACTTCAATA 
GTAGCAGAAG ACAAAAAAGG TCATCTAATA CATGGGAGAA ACATGGATTT TGGAGTATTT 
CTTGGGTGGA ACATAAATAA TGATACCTGG GTCATAACTG AGCAACTAAA ACCTTTAACA 
GTGAATTTGG ATTTCCAAAG AAACAACAAA ACTGTCTTCA AGGCTTCAAG CTTTGCTGGC 
TATGTGGGCA TGTTAACAGG ATTCAAACCA GGACTGTTCA GTCTTACACT GAATGAACGT 
TTCAGTATAA ATGGTGGTTA TCTGGGTATT CTAGAATGGA TTCTGGGAAA GAAAGATGTC 
ATGTGGATAG GGTTCCTCAC TAGAACAGTT CTGGAAAATA GCACAAGTTA TGAAGAAGCC 
AAGAATTTAT TGACCAAGAC CAAGATATTG GCCCCAGCCT ACTTTATCCT GGGAGGCAAC 
CAGTCTGGGG AAGGTTGTGT GATTACACGA GACAGAAAGG AATCATTGGA TGTATATGAA 
CTCGATGCTA AGCAGGGTAG ATGGTATGTG GTACAAACAA ATTATGACCG TTGGAAACAT 
CCCTTCTTCC TTGATGATCG CAGAACGCCT GCAAAGATGT GTCTGAACCG CACCAGCCAA 
GAGAATATCT CATTTGAAAC CATGTATGAT GTCCTGTCAA CAAAACCTGT CCTCAACAAG 
CTGACCGTAT ACACAACCTT GATAGATGTT ACCAAAGGTC AATTCGAAAC TTACCTGCGG 
GACTGCCCTG ACCCTTGTAT AGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH1 ( NM_001127505.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001120977.1 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA.

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CloneID	OHu20041
Clone ID Related Accession (Same CDS sequence)	NM_001127505.2 , NM_001127505.3
Accession Version	NM_001127505.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	acid ceramidase isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA260852.1, BC016828.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1, AI241768.1 and AC124242.6. On May 31, 2019 this sequence version replaced NM_001127505.2. Transcript Variant: This variant (3) contains an alternate in-frame exon and lacks another alternate in-frame exon compared to variant 2. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016828.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA 
GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACTGTCTTC 
CCTGCTGTTA TAAGGTACAG AGGTGCAGTT CCATGGTACA CCATAAATCT TGACTTACCA 
CCCTACAAAA GATGGCATGA ATTGATGCTT GACAAGGCAC CAGTGCCTGG CCTACTTGGC 
AACTTTCCTG GCCCTTTTGA AGAGGAAATG AAGGGTATTG CCGCTGTTAC TGATATACCT 
TTAGGAGAGA TTATTTCATT CAATATTTTT TATGAATTAT TTACCATTTG TACTTCAATA 
GTAGCAGAAG ACAAAAAAGG TCATCTAATA CATGGGAGAA ACATGGATTT TGGAGTATTT 
CTTGGGTGGA ACATAAATAA TGATACCTGG GTCATAACTG AGCAACTAAA ACCTTTAACA 
GTGAATTTGG ATTTCCAAAG AAACAACAAA ACTGTCTTCA AGGCTTCAAG CTTTGCTGGC 
TATGTGGGCA TGTTAACAGG ATTCAAACCA GGACTGTTCA GTCTTACACT GAATGAACGT 
TTCAGTATAA ATGGTGGTTA TCTGGGTATT CTAGAATGGA TTCTGGGAAA GAAAGATGTC 
ATGTGGATAG GGTTCCTCAC TAGAACAGTT CTGGAAAATA GCACAAGTTA TGAAGAAGCC 
AAGAATTTAT TGACCAAGAC CAAGATATTG GCCCCAGCCT ACTTTATCCT GGGAGGCAAC 
CAGTCTGGGG AAGGTTGTGT GATTACACGA GACAGAAAGG AATCATTGGA TGTATATGAA 
CTCGATGCTA AGCAGGGTAG ATGGTATGTG GTACAAACAA ATTATGACCG TTGGAAACAT 
CCCTTCTTCC TTGATGATCG CAGAACGCCT GCAAAGATGT GTCTGAACCG CACCAGCCAA 
GAGAATATCT CATTTGAAAC CATGTATGAT GTCCTGTCAA CAAAACCTGT CCTCAACAAG 
CTGACCGTAT ACACAACCTT GATAGATGTT ACCAAAGGTC AATTCGAAAC TTACCTGCGG 
GACTGCCCTG ACCCTTGTAT AGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120977.1
CDS	161..1330
Translation	MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPTVFPAVIRYRGAVPWYTINLDLPPYKRWHELMLDKAPVPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_001127505.3
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127505.3

ORF Insert Sequence:

ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA 
GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACTGTCTTC 
CCTGCTGTTA TAAGGTACAG AGGTGCAGTT CCATGGTACA CCATAAATCT TGACTTACCA 
CCCTACAAAA GATGGCATGA ATTGATGCTT GACAAGGCAC CAGTGCCTGG CCTACTTGGC 
AACTTTCCTG GCCCTTTTGA AGAGGAAATG AAGGGTATTG CCGCTGTTAC TGATATACCT 
TTAGGAGAGA TTATTTCATT CAATATTTTT TATGAATTAT TTACCATTTG TACTTCAATA 
GTAGCAGAAG ACAAAAAAGG TCATCTAATA CATGGGAGAA ACATGGATTT TGGAGTATTT 
CTTGGGTGGA ACATAAATAA TGATACCTGG GTCATAACTG AGCAACTAAA ACCTTTAACA 
GTGAATTTGG ATTTCCAAAG AAACAACAAA ACTGTCTTCA AGGCTTCAAG CTTTGCTGGC 
TATGTGGGCA TGTTAACAGG ATTCAAACCA GGACTGTTCA GTCTTACACT GAATGAACGT 
TTCAGTATAA ATGGTGGTTA TCTGGGTATT CTAGAATGGA TTCTGGGAAA GAAAGATGTC 
ATGTGGATAG GGTTCCTCAC TAGAACAGTT CTGGAAAATA GCACAAGTTA TGAAGAAGCC 
AAGAATTTAT TGACCAAGAC CAAGATATTG GCCCCAGCCT ACTTTATCCT GGGAGGCAAC 
CAGTCTGGGG AAGGTTGTGT GATTACACGA GACAGAAAGG AATCATTGGA TGTATATGAA 
CTCGATGCTA AGCAGGGTAG ATGGTATGTG GTACAAACAA ATTATGACCG TTGGAAACAT 
CCCTTCTTCC TTGATGATCG CAGAACGCCT GCAAAGATGT GTCTGAACCG CACCAGCCAA 
GAGAATATCT CATTTGAAAC CATGTATGAT GTCCTGTCAA CAAAACCTGT CCTCAACAAG 
CTGACCGTAT ACACAACCTT GATAGATGTT ACCAAAGGTC AATTCGAAAC TTACCTGCGG 
GACTGCCCTG ACCCTTGTAT AGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH1 ( NM_004315.5 ) cDNA ORF clone, Homo sapiens(Human) -> NP_004306.3 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA.

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CloneID	OHu20258
Clone ID Related Accession (Same CDS sequence)	NM_004315.5 , NM_004315.6
Accession Version	NM_004315.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	acid ceramidase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA260852.1, AK289481.1, AY305384.1, AK222780.1, BU633536.1 and AI241768.1. This sequence is a reference standard in the RefSeqGene project. On Nov 5, 2015 this sequence version replaced NM_004315.4. Transcript Variant: This variant (2) encodes the longest isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.110417.1, SRR1660809.122258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA 
GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACGTACAGA 
GGTGCAGTTC CATGGTACAC CATAAATCTT GACTTACCAC CCTACAAAAG ATGGCATGAA 
TTGATGCTTG ACAAGGCACC AGTGCTAAAG GTTATAGTGA ATTCTCTGAA GAATATGATA 
AATACATTCG TGCCAAGTGG AAAAATTATG CAGGTGGTGG ATGAAAAATT GCCTGGCCTA 
CTTGGCAACT TTCCTGGCCC TTTTGAAGAG GAAATGAAGG GTATTGCCGC TGTTACTGAT 
ATACCTTTAG GAGAGATTAT TTCATTCAAT ATTTTTTATG AATTATTTAC CATTTGTACT 
TCAATAGTAG CAGAAGACAA AAAAGGTCAT CTAATACATG GGAGAAACAT GGATTTTGGA 
GTATTTCTTG GGTGGAACAT AAATAATGAT ACCTGGGTCA TAACTGAGCA ACTAAAACCT 
TTAACAGTGA ATTTGGATTT CCAAAGAAAC AACAAAACTG TCTTCAAGGC TTCAAGCTTT 
GCTGGCTATG TGGGCATGTT AACAGGATTC AAACCAGGAC TGTTCAGTCT TACACTGAAT 
GAACGTTTCA GTATAAATGG TGGTTATCTG GGTATTCTAG AATGGATTCT GGGAAAGAAA 
GATGTCATGT GGATAGGGTT CCTCACTAGA ACAGTTCTGG AAAATAGCAC AAGTTATGAA 
GAAGCCAAGA ATTTATTGAC CAAGACCAAG ATATTGGCCC CAGCCTACTT TATCCTGGGA 
GGCAACCAGT CTGGGGAAGG TTGTGTGATT ACACGAGACA GAAAGGAATC ATTGGATGTA 
TATGAACTCG ATGCTAAGCA GGGTAGATGG TATGTGGTAC AAACAAATTA TGACCGTTGG 
AAACATCCCT TCTTCCTTGA TGATCGCAGA ACGCCTGCAA AGATGTGTCT GAACCGCACC 
AGCCAAGAGA ATATCTCATT TGAAACCATG TATGATGTCC TGTCAACAAA ACCTGTCCTC 
AACAAGCTGA CCGTATACAC AACCTTGATA GATGTTACCA AAGGTCAATT CGAAACTTAC 
CTGCGGGACT GCCCTGACCC TTGTATAGGT TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004306.3
CDS	198..1433
Translation	MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_004315.5
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004315.5

ORF Insert Sequence:

ATGAACTGCT GCATCGGGCT GGGAGAGAAA GCTCGCGGGT CCCACCGGGC CTCCTACCCA 
AGTCTCAGCG CGCTTTTCAC CGAGGCCTCA ATTCTGGGAT TTGGCAGCTT TGCTGTGAAA 
GCCCAATGGA CAGAGGACTG CAGAAAATCA ACCTATCCTC CTTCAGGACC AACGTACAGA 
GGTGCAGTTC CATGGTACAC CATAAATCTT GACTTACCAC CCTACAAAAG ATGGCATGAA 
TTGATGCTTG ACAAGGCACC AGTGCTAAAG GTTATAGTGA ATTCTCTGAA GAATATGATA 
AATACATTCG TGCCAAGTGG AAAAATTATG CAGGTGGTGG ATGAAAAATT GCCTGGCCTA 
CTTGGCAACT TTCCTGGCCC TTTTGAAGAG GAAATGAAGG GTATTGCCGC TGTTACTGAT 
ATACCTTTAG GAGAGATTAT TTCATTCAAT ATTTTTTATG AATTATTTAC CATTTGTACT 
TCAATAGTAG CAGAAGACAA AAAAGGTCAT CTAATACATG GGAGAAACAT GGATTTTGGA 
GTATTTCTTG GGTGGAACAT AAATAATGAT ACCTGGGTCA TAACTGAGCA ACTAAAACCT 
TTAACAGTGA ATTTGGATTT CCAAAGAAAC AACAAAACTG TCTTCAAGGC TTCAAGCTTT 
GCTGGCTATG TGGGCATGTT AACAGGATTC AAACCAGGAC TGTTCAGTCT TACACTGAAT 
GAACGTTTCA GTATAAATGG TGGTTATCTG GGTATTCTAG AATGGATTCT GGGAAAGAAA 
GATGTCATGT GGATAGGGTT CCTCACTAGA ACAGTTCTGG AAAATAGCAC AAGTTATGAA 
GAAGCCAAGA ATTTATTGAC CAAGACCAAG ATATTGGCCC CAGCCTACTT TATCCTGGGA 
GGCAACCAGT CTGGGGAAGG TTGTGTGATT ACACGAGACA GAAAGGAATC ATTGGATGTA 
TATGAACTCG ATGCTAAGCA GGGTAGATGG TATGTGGTAC AAACAAATTA TGACCGTTGG 
AAACATCCCT TCTTCCTTGA TGATCGCAGA ACGCCTGCAA AGATGTGTCT GAACCGCACC 
AGCCAAGAGA ATATCTCATT TGAAACCATG TATGATGTCC TGTCAACAAA ACCTGTCCTC 
AACAAGCTGA CCGTATACAC AACCTTGATA GATGTTACCA AAGGTCAATT CGAAACTTAC 
CTGCGGGACT GCCCTGACCC TTGTATAGGT TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH1 ( XM_005273504.3 ) cDNA ORF clone, Homo sapiens(Human) -> XP_005273561.1 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu42065
Clone ID Related Accession (Same CDS sequence)	XM_005273504.3
Accession Version	XM_005273504.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	acid ceramidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC AGTGGACAGA GGACTGCAGA AAATCAACCT ATCCTCCTTC AGGACCAACG 
TACAGAGGTG CAGTTCCATG GTACACCATA AATCTTGACT TACCACCCTA CAAAAGATGG 
CATGAATTGA TGCTTGACAA GGCACCAGTG CTAAAGGTTA TAGTGAATTC TCTGAAGAAT 
ATGATAAATA CATTCGTGCC AAGTGGAAAA ATTATGCAGG TGGTGGATGA AAAATTGCCT 
GGCCTACTTG GCAACTTTCC TGGCCCTTTT GAAGAGGAAA TGAAGGGTAT TGCCGCTGTT 
ACTGATATAC CTTTAGGAGA GATTATTTCA TTCAATATTT TTTATGAATT ATTTACCATT 
TGTACTTCAA TAGTAGCAGA AGACAAAAAA GGTCATCTAA TACATGGGAG AAACATGGAT 
TTTGGAGTAT TTCTTGGGTG GAACATAAAT AATGATACCT GGGTCATAAC TGAGCAACTA 
AAACCTTTAA CAGTGAATTT GGATTTCCAA AGAAACAACA AAACTGTCTT CAAGGCTTCA 
AGCTTTGCTG GCTATGTGGG CATGTTAACA GGATTCAAAC CAGGACTGTT CAGTCTTACA 
CTGAATGAAC GTTTCAGTAT AAATGGTGGT TATCTGGGTA TTCTAGAATG GATTCTGGGA 
AAGAAAGATG TCATGTGGAT AGGGTTCCTC ACTAGAACAG TTCTGGAAAA TAGCACAAGT 
TATGAAGAAG CCAAGAATTT ATTGACCAAG ACCAAGATAT TGGCCCCAGC CTACTTTATC 
CTGGGAGGCA ACCAGTCTGG GGAAGGTTGT GTGATTACAC GAGACAGAAA GGAATCATTG 
GATGTATATG AACTCGATGC TAAGCAGGGT AGATGGTATG TGGTACAAAC AAATTATGAC 
CGTTGGAAAC ATCCCTTCTT CCTTGATGAT CGCAGAACGC CTGCAAAGAT GTGTCTGAAC 
CGCACCAGCC AAGAGAATAT CTCATTTGAA ACCATGTATG ATGTCCTGTC AACAAAACCT 
GTCCTCAACA AGCTGACCGT ATACACAACC TTGATAGATG TTACCAAAGG TCAATTCGAA 
ACTTACCTGC GGGACTGCCC TGACCCTTGT ATAGGTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273561.1
CDS	140..1261
Translation	MEAQWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	XM_005273504.3
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273504.3

ORF Insert Sequence:

ATGGAGGCGC AGTGGACAGA GGACTGCAGA AAATCAACCT ATCCTCCTTC AGGACCAACG 
TACAGAGGTG CAGTTCCATG GTACACCATA AATCTTGACT TACCACCCTA CAAAAGATGG 
CATGAATTGA TGCTTGACAA GGCACCAGTG CTAAAGGTTA TAGTGAATTC TCTGAAGAAT 
ATGATAAATA CATTCGTGCC AAGTGGAAAA ATTATGCAGG TGGTGGATGA AAAATTGCCT 
GGCCTACTTG GCAACTTTCC TGGCCCTTTT GAAGAGGAAA TGAAGGGTAT TGCCGCTGTT 
ACTGATATAC CTTTAGGAGA GATTATTTCA TTCAATATTT TTTATGAATT ATTTACCATT 
TGTACTTCAA TAGTAGCAGA AGACAAAAAA GGTCATCTAA TACATGGGAG AAACATGGAT 
TTTGGAGTAT TTCTTGGGTG GAACATAAAT AATGATACCT GGGTCATAAC TGAGCAACTA 
AAACCTTTAA CAGTGAATTT GGATTTCCAA AGAAACAACA AAACTGTCTT CAAGGCTTCA 
AGCTTTGCTG GCTATGTGGG CATGTTAACA GGATTCAAAC CAGGACTGTT CAGTCTTACA 
CTGAATGAAC GTTTCAGTAT AAATGGTGGT TATCTGGGTA TTCTAGAATG GATTCTGGGA 
AAGAAAGATG TCATGTGGAT AGGGTTCCTC ACTAGAACAG TTCTGGAAAA TAGCACAAGT 
TATGAAGAAG CCAAGAATTT ATTGACCAAG ACCAAGATAT TGGCCCCAGC CTACTTTATC 
CTGGGAGGCA ACCAGTCTGG GGAAGGTTGT GTGATTACAC GAGACAGAAA GGAATCATTG 
GATGTATATG AACTCGATGC TAAGCAGGGT AGATGGTATG TGGTACAAAC AAATTATGAC 
CGTTGGAAAC ATCCCTTCTT CCTTGATGAT CGCAGAACGC CTGCAAAGAT GTGTCTGAAC 
CGCACCAGCC AAGAGAATAT CTCATTTGAA ACCATGTATG ATGTCCTGTC AACAAAACCT 
GTCCTCAACA AGCTGACCGT ATACACAACC TTGATAGATG TTACCAAAGG TCAATTCGAA 
ACTTACCTGC GGGACTGCCC TGACCCTTGT ATAGGTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH1 ( NM_001363743.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001350672.1 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu107073
Clone ID Related Accession (Same CDS sequence)	NM_001363743.1 , NM_001363743.2
Accession Version	NM_001363743.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	acid ceramidase isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124242.6. On May 29, 2018 this sequence version replaced XM_024447153.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.124746.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267760, SAMN03267764 [ECO:0000348] ##Evidence-Data-END##

ATGCTTGACA AGGCACCAGT GCTAAAGGTT ATAGTGAATT CTCTGAAGAA TATGATAAAT 
ACATTCGTGC CAAGTGGAAA AATTATGCAG GTGGTGGATG AAAAATTGCC TGGCCTACTT 
GGCAACTTTC CTGGCCCTTT TGAAGAGGAA ATGAAGGGTA TTGCCGCTGT TACTGATATA 
CCTTTAGGAG AGATTATTTC ATTCAATATT TTTTATGAAT TATTTACCAT TTGTACTTCA 
ATAGTAGCAG AAGACAAAAA AGGTCATCTA ATACATGGGA GAAACATGGA TTTTGGAGTA 
TTTCTTGGGT GGAACATAAA TAATGATACC TGGGTCATAA CTGAGCAACT AAAACCTTTA 
ACAGTGAATT TGGATTTCCA AAGAAACAAC AAAACTGTCT TCAAGGCTTC AAGCTTTGCT 
GGCTATGTGG GCATGTTAAC AGGATTCAAA CCAGGACTGT TCAGTCTTAC ACTGAATGAA 
CGTTTCAGTA TAAATGGTGG TTATCTGGGT ATTCTAGAAT GGATTCTGGG AAAGAAAGAT 
GTCATGTGGA TAGGGTTCCT CACTAGAACA GTTCTGGAAA ATAGCACAAG TTATGAAGAA 
GCCAAGAATT TATTGACCAA GACCAAGATA TTGGCCCCAG CCTACTTTAT CCTGGGAGGC 
AACCAGTCTG GGGAAGGTTG TGTGATTACA CGAGACAGAA AGGAATCATT GGATGTATAT 
GAACTCGATG CTAAGCAGGG TAGATGGTAT GTGGTACAAA CAAATTATGA CCGTTGGAAA 
CATCCCTTCT TCCTTGATGA TCGCAGAACG CCTGCAAAGA TGTGTCTGAA CCGCACCAGC 
CAAGAGAATA TCTCATTTGA AACCATGTAT GATGTCCTGT CAACAAAACC TGTCCTCAAC 
AAGCTGACCG TATACACAAC CTTGATAGAT GTTACCAAAG GTCAATTCGA AACTTACCTG 
CGGGACTGCC CTGACCCTTG TATAGGTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350672.1
CDS	193..1185
Translation	MLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_001363743.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363743.1

ORF Insert Sequence:

ATGCTTGACA AGGCACCAGT GCTAAAGGTT ATAGTGAATT CTCTGAAGAA TATGATAAAT 
ACATTCGTGC CAAGTGGAAA AATTATGCAG GTGGTGGATG AAAAATTGCC TGGCCTACTT 
GGCAACTTTC CTGGCCCTTT TGAAGAGGAA ATGAAGGGTA TTGCCGCTGT TACTGATATA 
CCTTTAGGAG AGATTATTTC ATTCAATATT TTTTATGAAT TATTTACCAT TTGTACTTCA 
ATAGTAGCAG AAGACAAAAA AGGTCATCTA ATACATGGGA GAAACATGGA TTTTGGAGTA 
TTTCTTGGGT GGAACATAAA TAATGATACC TGGGTCATAA CTGAGCAACT AAAACCTTTA 
ACAGTGAATT TGGATTTCCA AAGAAACAAC AAAACTGTCT TCAAGGCTTC AAGCTTTGCT 
GGCTATGTGG GCATGTTAAC AGGATTCAAA CCAGGACTGT TCAGTCTTAC ACTGAATGAA 
CGTTTCAGTA TAAATGGTGG TTATCTGGGT ATTCTAGAAT GGATTCTGGG AAAGAAAGAT 
GTCATGTGGA TAGGGTTCCT CACTAGAACA GTTCTGGAAA ATAGCACAAG TTATGAAGAA 
GCCAAGAATT TATTGACCAA GACCAAGATA TTGGCCCCAG CCTACTTTAT CCTGGGAGGC 
AACCAGTCTG GGGAAGGTTG TGTGATTACA CGAGACAGAA AGGAATCATT GGATGTATAT 
GAACTCGATG CTAAGCAGGG TAGATGGTAT GTGGTACAAA CAAATTATGA CCGTTGGAAA 
CATCCCTTCT TCCTTGATGA TCGCAGAACG CCTGCAAAGA TGTGTCTGAA CCGCACCAGC 
CAAGAGAATA TCTCATTTGA AACCATGTAT GATGTCCTGT CAACAAAACC TGTCCTCAAC 
AAGCTGACCG TATACACAAC CTTGATAGAT GTTACCAAAG GTCAATTCGA AACTTACCTG 
CGGGACTGCC CTGACCCTTG TATAGGTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASAH1 ( NM_001363743.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001350672.1 Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu107073
Clone ID Related Accession (Same CDS sequence)	NM_001363743.1 , NM_001363743.2
Accession Version	NM_001363743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Homo sapiens(human)
Product	acid ceramidase isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124242.6. On May 31, 2019 this sequence version replaced NM_001363743.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.124746.1, SRR1803616.178239.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267760, SAMN03267764 [ECO:0000348] ##Evidence-Data-END##

ATGCTTGACA AGGCACCAGT GCTAAAGGTT ATAGTGAATT CTCTGAAGAA TATGATAAAT 
ACATTCGTGC CAAGTGGAAA AATTATGCAG GTGGTGGATG AAAAATTGCC TGGCCTACTT 
GGCAACTTTC CTGGCCCTTT TGAAGAGGAA ATGAAGGGTA TTGCCGCTGT TACTGATATA 
CCTTTAGGAG AGATTATTTC ATTCAATATT TTTTATGAAT TATTTACCAT TTGTACTTCA 
ATAGTAGCAG AAGACAAAAA AGGTCATCTA ATACATGGGA GAAACATGGA TTTTGGAGTA 
TTTCTTGGGT GGAACATAAA TAATGATACC TGGGTCATAA CTGAGCAACT AAAACCTTTA 
ACAGTGAATT TGGATTTCCA AAGAAACAAC AAAACTGTCT TCAAGGCTTC AAGCTTTGCT 
GGCTATGTGG GCATGTTAAC AGGATTCAAA CCAGGACTGT TCAGTCTTAC ACTGAATGAA 
CGTTTCAGTA TAAATGGTGG TTATCTGGGT ATTCTAGAAT GGATTCTGGG AAAGAAAGAT 
GTCATGTGGA TAGGGTTCCT CACTAGAACA GTTCTGGAAA ATAGCACAAG TTATGAAGAA 
GCCAAGAATT TATTGACCAA GACCAAGATA TTGGCCCCAG CCTACTTTAT CCTGGGAGGC 
AACCAGTCTG GGGAAGGTTG TGTGATTACA CGAGACAGAA AGGAATCATT GGATGTATAT 
GAACTCGATG CTAAGCAGGG TAGATGGTAT GTGGTACAAA CAAATTATGA CCGTTGGAAA 
CATCCCTTCT TCCTTGATGA TCGCAGAACG CCTGCAAAGA TGTGTCTGAA CCGCACCAGC 
CAAGAGAATA TCTCATTTGA AACCATGTAT GATGTCCTGT CAACAAAACC TGTCCTCAAC 
AAGCTGACCG TATACACAAC CTTGATAGAT GTTACCAAAG GTCAATTCGA AACTTACCTG 
CGGGACTGCC CTGACCCTTG TATAGGTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350672.1
CDS	193..1185
Translation	MLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW

Target ORF information:

RefSeq Version	NM_001363743.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-acylsphingosine amidohydrolase 1 (ASAH1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363743.2

ORF Insert Sequence:

ATGCTTGACA AGGCACCAGT GCTAAAGGTT ATAGTGAATT CTCTGAAGAA TATGATAAAT 
ACATTCGTGC CAAGTGGAAA AATTATGCAG GTGGTGGATG AAAAATTGCC TGGCCTACTT 
GGCAACTTTC CTGGCCCTTT TGAAGAGGAA ATGAAGGGTA TTGCCGCTGT TACTGATATA 
CCTTTAGGAG AGATTATTTC ATTCAATATT TTTTATGAAT TATTTACCAT TTGTACTTCA 
ATAGTAGCAG AAGACAAAAA AGGTCATCTA ATACATGGGA GAAACATGGA TTTTGGAGTA 
TTTCTTGGGT GGAACATAAA TAATGATACC TGGGTCATAA CTGAGCAACT AAAACCTTTA 
ACAGTGAATT TGGATTTCCA AAGAAACAAC AAAACTGTCT TCAAGGCTTC AAGCTTTGCT 
GGCTATGTGG GCATGTTAAC AGGATTCAAA CCAGGACTGT TCAGTCTTAC ACTGAATGAA 
CGTTTCAGTA TAAATGGTGG TTATCTGGGT ATTCTAGAAT GGATTCTGGG AAAGAAAGAT 
GTCATGTGGA TAGGGTTCCT CACTAGAACA GTTCTGGAAA ATAGCACAAG TTATGAAGAA 
GCCAAGAATT TATTGACCAA GACCAAGATA TTGGCCCCAG CCTACTTTAT CCTGGGAGGC 
AACCAGTCTG GGGAAGGTTG TGTGATTACA CGAGACAGAA AGGAATCATT GGATGTATAT 
GAACTCGATG CTAAGCAGGG TAGATGGTAT GTGGTACAAA CAAATTATGA CCGTTGGAAA 
CATCCCTTCT TCCTTGATGA TCGCAGAACG CCTGCAAAGA TGTGTCTGAA CCGCACCAGC 
CAAGAGAATA TCTCATTTGA AACCATGTAT GATGTCCTGT CAACAAAACC TGTCCTCAAC 
AAGCTGACCG TATACACAAC CTTGATAGAT GTTACCAAAG GTCAATTCGA AACTTACCTG 
CGGGACTGCC CTGACCCTTG TATAGGTTGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ASAH1
Entrez Gene ID	427
Full Name	N-acylsphingosine amidohydrolase 1
Synonyms	AC,ACDase,ASAH,PHP,PHP32,SMAPME
General protein information	Preferred Names N-acylsphingosine amidohydrolase 1 Names acid ceramidase N-acylsphingosine amidohydrolase (acid ceramidase) 1 acid CDase acylsphingosine deacylase putative 32 kDa heart protein
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 8 Map Location: 8p22
Summary	This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. [provided by RefSeq, Oct 2015].
Disorder MIM:	613468
Disorder Html:	Farber lipogranulomatosis, 228000 (3); Spinal muscular atrophy with progressive myoclonic epilepsy, 159950 (3)

	ASAH1	XP_001098342.1
Caenorhabditis elegans (roundworm)	asah-1	NP_493173.1
Homo sapiens (human)	ASAH1	NP_808592.2
Mus musculus (house mouse)	Asah1	NP_062708.1
Gallus gallus (chicken)	ASAH1	NP_001006453.1
Xenopus tropicalis (tropical clawed frog)	asah1	XP_002940494.1
Canis lupus familiaris (dog)	ASAH1	XP_005630014.1
Bos taurus (cattle)	ASAH1	NP_001068927.1
Rattus norvegicus (Norway rat)	Asah1	NP_445859.2
Pan troglodytes (chimpanzee)	ASAH1	NP_001092030.1
Danio rerio (zebrafish)	asah1b	NP_956871.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ASAH1 cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

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