asah1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	asah1
Entrez Gene ID	100485700
Full Name	N-acylsphingosine amidohydrolase (acid ceramidase) 1
General protein information	Preferred Names N-acylsphingosine amidohydrolase (acid ceramidase) 1 Names acid ceramidase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940448.4	XP_002940494.1	acid ceramidase
XM_002940448.5	XP_002940494.1	acid ceramidase

Pathways from BioSystems

Homologies

	ASAH1	XP_001098342.1
Caenorhabditis elegans (roundworm)	asah-1	NP_493173.1
Homo sapiens (human)	ASAH1	NP_808592.2
Mus musculus (house mouse)	Asah1	NP_062708.1
Gallus gallus (chicken)	ASAH1	NP_001006453.1
Xenopus tropicalis (tropical clawed frog)	asah1	XP_002940494.1
Canis lupus familiaris (dog)	ASAH1	XP_005630014.1
Bos taurus (cattle)	ASAH1	NP_001068927.1
Rattus norvegicus (Norway rat)	Asah1	NP_445859.2
Pan troglodytes (chimpanzee)	ASAH1	NP_001092030.1
Danio rerio (zebrafish)	asah1b	NP_956871.1

Gene Ontology

The following asah1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asah1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04692	XM_002940448.5 Latest version!	Xenopus tropicalis N-acylsphingosine amidohydrolase (acid ceramidase) 1 (asah1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OXa04692	XM_002940448.4	Xenopus tropicalis N-acylsphingosine amidohydrolase (acid ceramidase) 1 (asah1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asah1 ( XM_002940448.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940494.1 Xenopus tropicalis N-acylsphingosine amidohydrolase (acid ceramidase) 1 (asah1), mRNA.

Price & Availability↑

CloneID	OXa04692
Clone ID Related Accession (Same CDS sequence)	XM_002940448.4 , XM_002940448.5
Accession Version	XM_002940448.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	acid ceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940448.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCTCCGTGCT GCTGCGGCTG CTACTGTGTA TGGTGTTGGC AGCAATAATC 
GGACTCGCAC AGGACGTGCC GCCGTACACA GAGGATTGCA GAAGTGGAAC TTACCCACCC 
ACGGGCCCCA CGTTCAAAGG AAATATATCA TGGTATACTG TAAATCTGGA CCAGGCGCCA 
CAGGAAAGGT GGCAAAAGCT TATAAGTGAA AAGAAAACAG CACTAAACAA TTTAATCCAA 
GCAATTAAAA ATCTGGCAAC GTCATTCTTC CCAGGTGAAA AGATAATACA GCTAGTAGAT 
AAGGAACTGC CTCTCCTTAT TGGAACACTT CCTTCTCCAT TTGGTGAAGA GATTAAAGGA 
GTTGCTGATG CCTCTGGGCT GCCGTTAGGG GAGGTGATGC TATTTAACAT ATTTTATGAA 
GTCTTCACAG TGTGTACTTC CGTTGTGGCA GAGGATAAAT CTGGAAAGTT ATATCATGCC 
AGAAATCTGG ATTTTGGGTT ATTCCTTGGA TGGGATGTAA AGAACAACTC ATGGATGGTC 
ACACAGTTAT TAAGGCCATT GGTTGTTAAT GTGGATTTCC AGAGAAATGG GAAAACGGTT 
TTTGTGTCCA CCAGTTTTGC TGGATATATT GGGATGCTAA CTGGAATGAA ACCTGGCGTC 
TTTTCTCTAA CAATGAATGA GCGCTTCAGC GTTGATGGAG GTTTCATTGG TGTCTTTGAA 
TGGATCCTTG GGAAGCGGGA TGGCATGTGG ATGAGCTTTC TCACTAGAAG TGTACTAGAG 
AATTCAACAA GTTACGAGGA AGCTAAGACT CTCTTATCCA AAACAAAGTT GCTGGCTCCA 
GCGTATTTTA TATTGGGAGG CAACAAATCC GGAGAGGCTT GTGTGATCAC ACGTTCCAGG 
GCGTCCTGCC TTGATATCTG GGAGCTGGAT TTAAAAGAAG GAAGATGGTA TGTCCTGGAA 
ACAAACTATG ACCGCTGGAA GGCACCACTA CCCATTGATA ACAGAAGGGA CCCAGCCATG 
AAATGCATGG GAAGGACTTC TCAACAGAAC ATCTCATTTG GAACCATCTA TGATATGCTT 
TCAACCAAAC CTGTCCTCAA CAAGCTCACT GCATATACAA CACTGATGTC GGTTTCTGAT 
AACAAGCTAG AAGCGTACCT GCGCATCTGC CCAGATCCCT GCATGCCATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940494.1
CDS	123..1316
Translation	MAGGSVLLRLLLCMVLAAIIGLAQDVPPYTEDCRSGTYPPTGPTFKGNISWYTVNLDQAPQERWQKLISEKKTALNNLIQAIKNLATSFFPGEKIIQLVDKELPLLIGTLPSPFGEEIKGVADASGLPLGEVMLFNIFYEVFTVCTSVVAEDKSGKLYHARNLDFGLFLGWDVKNNSWMVTQLLRPLVVNVDFQRNGKTVFVSTSFAGYIGMLTGMKPGVFSLTMNERFSVDGGFIGVFEWILGKRDGMWMSFLTRSVLENSTSYEEAKTLLSKTKLLAPAYFILGGNKSGEACVITRSRASCLDIWELDLKEGRWYVLETNYDRWKAPLPIDNRRDPAMKCMGRTSQQNISFGTIYDMLSTKPVLNKLTAYTTLMSVSDNKLEAYLRICPDPCMPW

Target ORF information:

RefSeq Version	XM_002940448.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis N-acylsphingosine amidohydrolase (acid ceramidase) 1 (asah1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940448.4

ORF Insert Sequence:

ATGGCTGGGG GCTCCGTGCT GCTGCGGCTG CTACTGTGTA TGGTGTTGGC AGCAATAATC 
GGACTCGCAC AGGACGTGCC GCCGTACACA GAGGATTGCA GAAGTGGAAC TTACCCACCC 
ACGGGCCCCA CGTTCAAAGG AAATATATCA TGGTATACTG TAAATCTGGA CCAGGCGCCA 
CAGGAAAGGT GGCAAAAGCT TATAAGTGAA AAGAAAACAG CACTAAACAA TTTAATCCAA 
GCAATTAAAA ATCTGGCAAC GTCATTCTTC CCAGGTGAAA AGATAATACA GCTAGTAGAT 
AAGGAACTGC CTCTCCTTAT TGGAACACTT CCTTCTCCAT TTGGTGAAGA GATTAAAGGA 
GTTGCTGATG CCTCTGGGCT GCCGTTAGGG GAGGTGATGC TATTTAACAT ATTTTATGAA 
GTCTTCACAG TGTGTACTTC CGTTGTGGCA GAGGATAAAT CTGGAAAGTT ATATCATGCC 
AGAAATCTGG ATTTTGGGTT ATTCCTTGGA TGGGATGTAA AGAACAACTC ATGGATGGTC 
ACACAGTTAT TAAGGCCATT GGTTGTTAAT GTGGATTTCC AGAGAAATGG GAAAACGGTT 
TTTGTGTCCA CCAGTTTTGC TGGATATATT GGGATGCTAA CTGGAATGAA ACCTGGCGTC 
TTTTCTCTAA CAATGAATGA GCGCTTCAGC GTTGATGGAG GTTTCATTGG TGTCTTTGAA 
TGGATCCTTG GGAAGCGGGA TGGCATGTGG ATGAGCTTTC TCACTAGAAG TGTACTAGAG 
AATTCAACAA GTTACGAGGA AGCTAAGACT CTCTTATCCA AAACAAAGTT GCTGGCTCCA 
GCGTATTTTA TATTGGGAGG CAACAAATCC GGAGAGGCTT GTGTGATCAC ACGTTCCAGG 
GCGTCCTGCC TTGATATCTG GGAGCTGGAT TTAAAAGAAG GAAGATGGTA TGTCCTGGAA 
ACAAACTATG ACCGCTGGAA GGCACCACTA CCCATTGATA ACAGAAGGGA CCCAGCCATG 
AAATGCATGG GAAGGACTTC TCAACAGAAC ATCTCATTTG GAACCATCTA TGATATGCTT 
TCAACCAAAC CTGTCCTCAA CAAGCTCACT GCATATACAA CACTGATGTC GGTTTCTGAT 
AACAAGCTAG AAGCGTACCT GCGCATCTGC CCAGATCCCT GCATGCCATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asah1 ( XM_002940448.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940494.1 Xenopus tropicalis N-acylsphingosine amidohydrolase (acid ceramidase) 1 (asah1), mRNA.

Price & Availability↑

CloneID	OXa04692
Clone ID Related Accession (Same CDS sequence)	XM_002940448.4 , XM_002940448.5
Accession Version	XM_002940448.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	acid ceramidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940448.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCTCCGTGCT GCTGCGGCTG CTACTGTGTA TGGTGTTGGC AGCAATAATC 
GGACTCGCAC AGGACGTGCC GCCGTACACA GAGGATTGCA GAAGTGGAAC TTACCCACCC 
ACGGGCCCCA CGTTCAAAGG AAATATATCA TGGTATACTG TAAATCTGGA CCAGGCGCCA 
CAGGAAAGGT GGCAAAAGCT TATAAGTGAA AAGAAAACAG CACTAAACAA TTTAATCCAA 
GCAATTAAAA ATCTGGCAAC GTCATTCTTC CCAGGTGAAA AGATAATACA GCTAGTAGAT 
AAGGAACTGC CTCTCCTTAT TGGAACACTT CCTTCTCCAT TTGGTGAAGA GATTAAAGGA 
GTTGCTGATG CCTCTGGGCT GCCGTTAGGG GAGGTGATGC TATTTAACAT ATTTTATGAA 
GTCTTCACAG TGTGTACTTC CGTTGTGGCA GAGGATAAAT CTGGAAAGTT ATATCATGCC 
AGAAATCTGG ATTTTGGGTT ATTCCTTGGA TGGGATGTAA AGAACAACTC ATGGATGGTC 
ACACAGTTAT TAAGGCCATT GGTTGTTAAT GTGGATTTCC AGAGAAATGG GAAAACGGTT 
TTTGTGTCCA CCAGTTTTGC TGGATATATT GGGATGCTAA CTGGAATGAA ACCTGGCGTC 
TTTTCTCTAA CAATGAATGA GCGCTTCAGC GTTGATGGAG GTTTCATTGG TGTCTTTGAA 
TGGATCCTTG GGAAGCGGGA TGGCATGTGG ATGAGCTTTC TCACTAGAAG TGTACTAGAG 
AATTCAACAA GTTACGAGGA AGCTAAGACT CTCTTATCCA AAACAAAGTT GCTGGCTCCA 
GCGTATTTTA TATTGGGAGG CAACAAATCC GGAGAGGCTT GTGTGATCAC ACGTTCCAGG 
GCGTCCTGCC TTGATATCTG GGAGCTGGAT TTAAAAGAAG GAAGATGGTA TGTCCTGGAA 
ACAAACTATG ACCGCTGGAA GGCACCACTA CCCATTGATA ACAGAAGGGA CCCAGCCATG 
AAATGCATGG GAAGGACTTC TCAACAGAAC ATCTCATTTG GAACCATCTA TGATATGCTT 
TCAACCAAAC CTGTCCTCAA CAAGCTCACT GCATATACAA CACTGATGTC GGTTTCTGAT 
AACAAGCTAG AAGCGTACCT GCGCATCTGC CCAGATCCCT GCATGCCATG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940494.1
CDS	110..1303
Translation	MAGGSVLLRLLLCMVLAAIIGLAQDVPPYTEDCRSGTYPPTGPTFKGNISWYTVNLDQAPQERWQKLISEKKTALNNLIQAIKNLATSFFPGEKIIQLVDKELPLLIGTLPSPFGEEIKGVADASGLPLGEVMLFNIFYEVFTVCTSVVAEDKSGKLYHARNLDFGLFLGWDVKNNSWMVTQLLRPLVVNVDFQRNGKTVFVSTSFAGYIGMLTGMKPGVFSLTMNERFSVDGGFIGVFEWILGKRDGMWMSFLTRSVLENSTSYEEAKTLLSKTKLLAPAYFILGGNKSGEACVITRSRASCLDIWELDLKEGRWYVLETNYDRWKAPLPIDNRRDPAMKCMGRTSQQNISFGTIYDMLSTKPVLNKLTAYTTLMSVSDNKLEAYLRICPDPCMPW

Target ORF information:

RefSeq Version	XM_002940448.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis N-acylsphingosine amidohydrolase (acid ceramidase) 1 (asah1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940448.5

ORF Insert Sequence:

ATGGCTGGGG GCTCCGTGCT GCTGCGGCTG CTACTGTGTA TGGTGTTGGC AGCAATAATC 
GGACTCGCAC AGGACGTGCC GCCGTACACA GAGGATTGCA GAAGTGGAAC TTACCCACCC 
ACGGGCCCCA CGTTCAAAGG AAATATATCA TGGTATACTG TAAATCTGGA CCAGGCGCCA 
CAGGAAAGGT GGCAAAAGCT TATAAGTGAA AAGAAAACAG CACTAAACAA TTTAATCCAA 
GCAATTAAAA ATCTGGCAAC GTCATTCTTC CCAGGTGAAA AGATAATACA GCTAGTAGAT 
AAGGAACTGC CTCTCCTTAT TGGAACACTT CCTTCTCCAT TTGGTGAAGA GATTAAAGGA 
GTTGCTGATG CCTCTGGGCT GCCGTTAGGG GAGGTGATGC TATTTAACAT ATTTTATGAA 
GTCTTCACAG TGTGTACTTC CGTTGTGGCA GAGGATAAAT CTGGAAAGTT ATATCATGCC 
AGAAATCTGG ATTTTGGGTT ATTCCTTGGA TGGGATGTAA AGAACAACTC ATGGATGGTC 
ACACAGTTAT TAAGGCCATT GGTTGTTAAT GTGGATTTCC AGAGAAATGG GAAAACGGTT 
TTTGTGTCCA CCAGTTTTGC TGGATATATT GGGATGCTAA CTGGAATGAA ACCTGGCGTC 
TTTTCTCTAA CAATGAATGA GCGCTTCAGC GTTGATGGAG GTTTCATTGG TGTCTTTGAA 
TGGATCCTTG GGAAGCGGGA TGGCATGTGG ATGAGCTTTC TCACTAGAAG TGTACTAGAG 
AATTCAACAA GTTACGAGGA AGCTAAGACT CTCTTATCCA AAACAAAGTT GCTGGCTCCA 
GCGTATTTTA TATTGGGAGG CAACAAATCC GGAGAGGCTT GTGTGATCAC ACGTTCCAGG 
GCGTCCTGCC TTGATATCTG GGAGCTGGAT TTAAAAGAAG GAAGATGGTA TGTCCTGGAA 
ACAAACTATG ACCGCTGGAA GGCACCACTA CCCATTGATA ACAGAAGGGA CCCAGCCATG 
AAATGCATGG GAAGGACTTC TCAACAGAAC ATCTCATTTG GAACCATCTA TGATATGCTT 
TCAACCAAAC CTGTCCTCAA CAAGCTCACT GCATATACAA CACTGATGTC GGTTTCTGAT 
AACAAGCTAG AAGCGTACCT GCGCATCTGC CCAGATCCCT GCATGCCATG GTGA