KRT83 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KRT83
Entrez Gene ID	3889
Full Name	keratin 83
Synonyms	EKVP5,HB3,Hb-3,KRTHB3,MNLIX
General protein information	Preferred Names keratin 83 Names keratin, type II cuticular Hb3 K83 hHb3 hair keratin K2.10 hard keratin, type II, 3 keratin 83, type II keratin, hair, basic, 3 type II hair keratin Hb3 type-II keratin Kb23
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.13
Summary	The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB1 and KRTHB6, is found primarily in the hair cortex. [provided by RefSeq, Jul 2008].
Disorder MIM:	602765
Disorder Html:	Monilethrix, 158000 (3); Erythrokeratodermia variabilis et progressiva 5, 617756 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002282.3	NP_002273.3	keratin, type II cuticular Hb3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KRT83	NP_001076854.1
Rattus norvegicus (Norway rat)	Kb23	NP_001008813.1
	KRT83	XP_001093322.1
Homo sapiens (human)	KRT83	NP_002273.3
Pan troglodytes (chimpanzee)	KRT83	XP_003313733.1
Mus musculus (house mouse)	5430421N21Rik	NP_001188252.1

Gene Ontology
Bibliography

Related articles in PubMed

Recessive progressive symmetric erythrokeratoderma results from a homozygous loss-of-function mutation of
Shah K, Ansar M, Mughal ZU, Khan FS, Ahmad W, Ferrara TM, Spritz RA
Journal of medical genetics54(3)186-189(2017 Mar)

Novel KRT83 and KRT86 mutations associated with monilethrix.
van Steensel M, Vreeburg M, Urbina MT, L?pez P, Morice-Picard F, van Geel M
Experimental dermatology24(3)222-4(2015 Mar)

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua R, Di Ilio C, Sacchetta P, Urbani A, Del Boccio P
Molecular bioSystems9(6)1108-16(2013 Jun)

Hair greying is associated with active hair growth.
Choi HI, Choi GI, Kim EK, Choi YJ, Sohn KC, Lee Y, Kim CD, Yoon TJ, Sohn HJ, Han SH, Kim S, Lee JH, Lee YH
The British journal of dermatology165(6)1183-9(2011 Dec)

New consensus nomenclature for mammalian keratins.
Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW
The Journal of cell biology174(2)169-74(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings indicate that at least some cases of autosomal recessive Progressive symmetric erythrokeratoderma (PSEK)and autosomal dominant monilethrix are allelic, respectively resulting from loss-of-function and missense mutations in the KRT83 gene
Title: Recessive progressive symmetric erythrokeratoderma results from a homozygous loss-of-function mutation of

Novel mutations causing monilethrix are reported in KRT81, KRT83, and KRT86.
Title: Novel KRT83 and KRT86 mutations associated with monilethrix.

A heterozygous missense mutation in hHb3 causing the substitution of a glutamic acid by a lysine at position 407 in the helix termination motif defines this particular residue as a trichocyte keratin mutational hotspot associated with monilethrix.
Title: A missense mutation in the type II hair keratin hHb3 is associated with monilethrix.

The KRTHB3 expression uniformly occurred in the midcortex region.
Title: Keratins of the human hair follicle.

The following KRT83 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT83 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu19697	NM_002282.3 Latest version!	Homo sapiens keratin 83 (KRT83), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KRT83 ( NM_002282.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002273.3 Homo sapiens keratin 83 (KRT83), mRNA.

Price & Availability↑

CloneID	OHu19697
Clone ID Related Accession (Same CDS sequence)	NM_002282.3
Accession Version	NM_002282.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	keratin, type II cuticular Hb3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Michael Rogers. The reference sequence was derived from AC121757.8 and BC069546.1. This sequence is a reference standard in the RefSeqGene project. On or before Feb 9, 2014 this sequence version replaced XM_006709960.1, XM_003846559.2, NM_002282.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: X99141.1, BC128063.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145313, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000293670.3/ ENSP00000293670.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCTGTG GCTTCAACTC CATAGGCTGT GGGTTCCGCC CTGGAAACTT CAGCTGTGTC 
TCTGCCTGCG GGCCCCGGCC AAGCCGCTGC TGCATCACCG CCGCCCCCTA CCGCGGCATC 
TCCTGCTACC GCGGCCTCAC CGGGGGCTTT GGCAGCCACA GCGTGTGCGG GGGCTTCCGC 
GCCGGCTCCT GCGGACGCAG CTTCGGCTAC CGCTCCGGGG GCGTGTGCGG ACCCAGCCCC 
CCATGCATCA CCACCGTGTC GGTCAACGAG AGCCTCCTCA CGCCCCTCAA CCTGGAGATA 
GACCCCAACG CGCAGTGCGT GAAGCAGGAG GAGAAGGAGC AGATCAAGTC CCTCAACAGC 
AGATTCGCGG CCTTCATCGA CAAGGTGCGC TTCCTGGAGC AGCAGAACAA GCTGCTGGAG 
ACAAAGCTGC AGTTCTACCA AAACCGCGAG TGCTGCCAGA GTAACCTGGA GCCCCTGTTT 
GCTGGCTACA TCGAGACTCT GCGGCGGGAG GCCGAGTGCG TGGAGGCTGA CAGTGGGAGG 
CTGGCCTCAG AGCTCAACCA CGTGCAGGAG GTGCTGGAGG GCTACAAGAA GAAGTATGAA 
GAAGAAGTAG CACTTCGAGC CACAGCAGAG AACGAGTTTG TGGCTCTAAA GAAGGATGTG 
GACTGCGCCT ACCTCCGCAA GTCAGACCTG GAGGCCAACG TGGAGGCCCT GATCCAGGAG 
ATTGACTTCC TGAGGCGGCT GTACGAGGAG GAGATCCGCA TTCTCCAATC CCACATCTCA 
GACACCTCCG TGGTTGTCAA GCTGGACAAC AGCCGGGACC TGAACATGGA CTGCATCGTT 
GCCGAGATCA AGGCACAGTA TGATGACATT GCCACCCGTA GCCGGGCTGA GGCCGAGTCC 
TGGTATCGCA GCAAGTGTGA GGAGATGAAG GCCACAGTGA TCAGGCACGG GGAGACCCTG 
CGCCGCACCA AGGAGGAGAT CAACGAGCTG AACCGCATGA TCCAGAGGCT GACAGCCGAG 
GTGGAGAATG CCAAGTGCCA GAACTCCAAG CTGGAAGCTG CGGTGGCCCA GTCTGAGCAG 
CAGGGTGAGG CGGCCCTCAG TGATGCCCGC TGCAAGCTGG CCGAGCTGGA GGGCGCCCTG 
CAGAAGGCCA AGCAAGACAT GGCCTGCCTG ATCAGGGAGT ACCAGGAGGT GATGAACTCC 
AAGCTAGGCC TGGATATCGA GATCGCCACC TACAGGCGCC TGCTGGAGGG CGAGGAGCAG 
AGGCTGTGTG AAGGTGTTGA AGCTGTGAAT GTCTGTGTCA GCAGCTCCCG GGGTGGGGTT 
GTGTGCGGGG ATCTCTGCGT GTCGGGCTCC CGGCCGGTGA CGGGCAGCGT CTGCAGTGCC 
CCCTGCAACG GGAACCTGGT GGTGAGCACT GGTTTGTGCA AGCCCTGTGG CCAGCTGAAC 
ACCACCTGTG GAGGGGGCTC CTGCGGCCAG GGGAGGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002273.3
CDS	64..1545
Translation	MTCGFNSIGCGFRPGNFSCVSACGPRPSRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFAGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEIRILQSHISDTSVVVKLDNSRDLNMDCIVAEIKAQYDDIATRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGVEAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNLVVSTGLCKPCGQLNTTCGGGSCGQGRH

Target ORF information:

RefSeq Version	NM_002282.3
Organism	Homo sapiens(human)
Definition	Homo sapiens keratin 83 (KRT83), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002282.3

ORF Insert Sequence:

ATGACCTGTG GCTTCAACTC CATAGGCTGT GGGTTCCGCC CTGGAAACTT CAGCTGTGTC 
TCTGCCTGCG GGCCCCGGCC AAGCCGCTGC TGCATCACCG CCGCCCCCTA CCGCGGCATC 
TCCTGCTACC GCGGCCTCAC CGGGGGCTTT GGCAGCCACA GCGTGTGCGG GGGCTTCCGC 
GCCGGCTCCT GCGGACGCAG CTTCGGCTAC CGCTCCGGGG GCGTGTGCGG ACCCAGCCCC 
CCATGCATCA CCACCGTGTC GGTCAACGAG AGCCTCCTCA CGCCCCTCAA CCTGGAGATA 
GACCCCAACG CGCAGTGCGT GAAGCAGGAG GAGAAGGAGC AGATCAAGTC CCTCAACAGC 
AGATTCGCGG CCTTCATCGA CAAGGTGCGC TTCCTGGAGC AGCAGAACAA GCTGCTGGAG 
ACAAAGCTGC AGTTCTACCA AAACCGCGAG TGCTGCCAGA GTAACCTGGA GCCCCTGTTT 
GCTGGCTACA TCGAGACTCT GCGGCGGGAG GCCGAGTGCG TGGAGGCTGA CAGTGGGAGG 
CTGGCCTCAG AGCTCAACCA CGTGCAGGAG GTGCTGGAGG GCTACAAGAA GAAGTATGAA 
GAAGAAGTAG CACTTCGAGC CACAGCAGAG AACGAGTTTG TGGCTCTAAA GAAGGATGTG 
GACTGCGCCT ACCTCCGCAA GTCAGACCTG GAGGCCAACG TGGAGGCCCT GATCCAGGAG 
ATTGACTTCC TGAGGCGGCT GTACGAGGAG GAGATCCGCA TTCTCCAATC CCACATCTCA 
GACACCTCCG TGGTTGTCAA GCTGGACAAC AGCCGGGACC TGAACATGGA CTGCATCGTT 
GCCGAGATCA AGGCACAGTA TGATGACATT GCCACCCGTA GCCGGGCTGA GGCCGAGTCC 
TGGTATCGCA GCAAGTGTGA GGAGATGAAG GCCACAGTGA TCAGGCACGG GGAGACCCTG 
CGCCGCACCA AGGAGGAGAT CAACGAGCTG AACCGCATGA TCCAGAGGCT GACAGCCGAG 
GTGGAGAATG CCAAGTGCCA GAACTCCAAG CTGGAAGCTG CGGTGGCCCA GTCTGAGCAG 
CAGGGTGAGG CGGCCCTCAG TGATGCCCGC TGCAAGCTGG CCGAGCTGGA GGGCGCCCTG 
CAGAAGGCCA AGCAAGACAT GGCCTGCCTG ATCAGGGAGT ACCAGGAGGT GATGAACTCC 
AAGCTAGGCC TGGATATCGA GATCGCCACC TACAGGCGCC TGCTGGAGGG CGAGGAGCAG 
AGGCTGTGTG AAGGTGTTGA AGCTGTGAAT GTCTGTGTCA GCAGCTCCCG GGGTGGGGTT 
GTGTGCGGGG ATCTCTGCGT GTCGGGCTCC CGGCCGGTGA CGGGCAGCGT CTGCAGTGCC 
CCCTGCAACG GGAACCTGGT GGTGAGCACT GGTTTGTGCA AGCCCTGTGG CCAGCTGAAC 
ACCACCTGTG GAGGGGGCTC CTGCGGCCAG GGGAGGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Recessive progressive symmetric erythrokeratoderma results from a homozygous loss-of-function mutation of
Journal of medical genetics54(3)186-189(2017 Mar)
Shah K,Ansar M,Mughal ZU,Khan FS,Ahmad W,Ferrara TM,Spritz RA

Novel KRT83 and KRT86 mutations associated with monilethrix.
Experimental dermatology24(3)222-4(2015 Mar)
van Steensel M,Vreeburg M,Urbina MT,L?pez P,Morice-Picard F,van Geel M

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Molecular bioSystems9(6)1108-16(2013 Jun)
Pieragostino D,Agnifili L,Fasanella V,D'Aguanno S,Mastropasqua R,Di Ilio C,Sacchetta P,Urbani A,Del Boccio P

Hair greying is associated with active hair growth.
The British journal of dermatology165(6)1183-9(2011 Dec)
Choi HI,Choi GI,Kim EK,Choi YJ,Sohn KC,Lee Y,Kim CD,Yoon TJ,Sohn HJ,Han SH,Kim S,Lee JH,Lee YH

New consensus nomenclature for mammalian keratins.
The Journal of cell biology174(2)169-74(2006 Jul)
Schweizer J,Bowden PE,Coulombe PA,Langbein L,Lane EB,Magin TM,Maltais L,Omary MB,Parry DA,Rogers MA,Wright MW

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KRT83 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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