CENPS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CENPS
Entrez Gene ID	378708
Full Name	centromere protein S
Synonyms	APITD1,CENP-S,FAAP16,MHF1
General protein information	Preferred Names centromere protein S Names centromere protein S FANCM associated histone fold protein 1 FANCM-interacting histone fold protein 1 Fanconi anemia-associated polypeptide of 16 kDa apoptosis-inducing TAF9-like domain-containing protein 1 apoptosis-inducing, TAF9-like domain 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.22
Summary	This gene was identified in the neuroblastoma tumor suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumors, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway. The protein is a component of multiple complexes, including the Fanconi anemia (FA) core complex, the APITD1/CENPS complex, and the CENPA-CAD (nucleosome distal) complex. Known functions include an involvement with chromatin associations of the FA core complex, and a role in the stable assembly of the outer kinetochore. Alternative splicing of this gene results in multiple transcript variants. Naturally occurring read-through transcripts also exist between this gene and the downstream cortistatin (CORT) gene, as represented in GeneID:100526739. An APITD1-related pseudogene has been identified on chromosome 7. [provided by RefSeq, Nov 2010].
Disorder MIM:	609130

mRNA and Protein(s)

mRNA	Protein	Name
NM_199294.3	NP_954988.1	centromere protein S
NM_199294.2	NP_954988.1	centromere protein S

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	APITD1	NP_001191858.1
Xenopus tropicalis (tropical clawed frog)	apitd1	NP_001165085.1
Bos taurus (cattle)	APITD1	NP_001033665.1
Danio rerio (zebrafish)	apitd1	NP_001122221.1
Homo sapiens (human)	APITD1-CORT	NP_001257446.1
Homo sapiens (human)	APITD1	NP_954988.1
Pan troglodytes (chimpanzee)	APITD1	NP_001191821.1
Mus musculus (house mouse)	Apitd1	NP_081539.1
Rattus norvegicus (Norway rat)	Apitd1	NP_001191808.1
Gallus gallus (chicken)	APITD1	NP_001191861.1

Gene Ontology
Bibliography

Related articles in PubMed

FANCM-associated proteins MHF1 and MHF2, but not the other Fanconi anemia factors, limit meiotic crossovers.
Girard C, Crismani W, Froger N, Mazel J, Lemhemdi A, Horlow C, Mercier R
Nucleic acids research42(14)9087-95(2014 Aug)

The histone-fold complex MHF is remodeled by FANCM to recognize branched DNA and protect genome stability.
Fox D 3rd, Yan Z, Ling C, Zhao Y, Lee DY, Fukagawa T, Yang W, Wang W
Cell research24(5)560-75(2014 May)

A CENP-S/X complex assembles at the centromere in S and G2 phases of the human cell cycle.
Dornblut C, Quinn N, Monajambashi S, Prendergast L, van Vuuren C, M?nch S, Deng W, Leonhardt H, Cardoso MC, Hoischen C, Diekmann S, Sullivan KF
Open biology4130229(2014 Feb)

The MHF complex senses branched DNA by binding a pair of crossover DNA duplexes.
Zhao Q, Saro D, Sachpatzidis A, Singh TR, Schlingman D, Zheng XF, Mack A, Tsai MS, Mochrie S, Regan L, Meetei AR, Sung P, Xiong Y
Nature communications52987(2014)

Emerging roles for centromere-associated proteins in DNA repair and genetic recombination.
Osman F, Whitby MC
Biochemical Society transactions41(6)1726-30(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The MHF complex, which is a heterotetramer that comprises two MHF1-MHF2 heterodimers, is remodeled by FANCM to favor recognition of branched DNA over dsDNA.
Title: The histone-fold complex MHF is remodeled by FANCM to recognize branched DNA and protect genome stability.

CENP-S is not found in a soluble complex with its binding partner CENP-T but it interacts strongly and specifically with immobilized CENP-T in an in vivo binding assay.
Title: A CENP-S/X complex assembles at the centromere in S and G2 phases of the human cell cycle.

MHF prefers branched DNA over dsDNA because it engages two duplex arms. MHF engages DNA forks or various four-way junctions independent of the junction-site structure. The DNA-binding interface of MHF is important for cellular resistance to DNA damage.
Title: The MHF complex senses branched DNA by binding a pair of crossover DNA duplexes.

It discusses current knowledge of the biological roles of CENP-S and CENP-X and how their dual existence may be a common feature of CCAN (constitutive centromere-associated network) proteins.
Title: Emerging roles for centromere-associated proteins in DNA repair and genetic recombination.

A long, positively charged patch exposed on the surface of the (MHF1-MHF2) complex plays a critical role in double-stranded DNA binding to chromatin.
Title: Structural peculiarities of the (MHF1-MHF2)4 octamer provide a long DNA binding patch to anchor the MHF-FANCM complex to chromatin: a solution SAXS study.

The following CENPS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CENPS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07808	NM_199294.3 Latest version!	Homo sapiens centromere protein S (CENPS), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu07808	NM_199294.2	Homo sapiens centromere protein S (CENPS), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CENPS ( NM_199294.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_954988.1 Homo sapiens centromere protein S (CENPS), transcript variant A, mRNA.

Price & Availability↑

CloneID	OHu07808
Clone ID Related Accession (Same CDS sequence)	NM_199294.3 , NM_199294.2
Accession Version	NM_199294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	centromere protein S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139424.21, BC029430.1 and AL354956.13. On Nov 22, 2018 this sequence version replaced NM_199294.2. Transcript Variant: This variant (A) represents the shorter transcript but encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.87438.1, SRR5189667.273523.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000309048.8/ ENSP00000308583.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGAGG AGGCGGAGAC CGAGGAGCAG CAGCGATTCT CTTACCAACA GAGGCTAAAG 
GCAGCAGTTC ACTATACTGT GGGTTGTCTT TGCGAGGAAG TTGCATTGGA CAAAGAGATG 
CAGTTCAGCA AACAGACCAT TGCGGCCATT TCGGAGCTGA CTTTCCGACA GTGTGAAAAT 
TTTGCCAAAG ACCTTGAAAT GTTTGCAAGA CATGCGAAAA GAACCACAAT TAACACTGAA 
GATGTGAAGC TCTTAGCCAG GAGGAGTAAT TCACTGCTAA AATACATCAC AGACAAAAGT 
GAAGAGATTG CTCAGATTAA CCTAGAACGA AAAGCACAGA AGAAAAAGAA GTCAGAGGAT 
GGAAGCAAAA ATTCAAGGCA GCCAGCAGAG GCTGGAGTGG TGGAAAGTGA GAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954988.1
CDS	86..502
Translation	MEEEAETEEQQRFSYQQRLKAAVHYTVGCLCEEVALDKEMQFSKQTIAAISELTFRQCENFAKDLEMFARHAKRTTINTEDVKLLARRSNSLLKYITDKSEEIAQINLERKAQKKKKSEDGSKNSRQPAEAGVVESEN

Target ORF information:

RefSeq Version	NM_199294.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein S (CENPS), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199294.3

ORF Insert Sequence:

ATGGAGGAGG AGGCGGAGAC CGAGGAGCAG CAGCGATTCT CTTACCAACA GAGGCTAAAG 
GCAGCAGTTC ACTATACTGT GGGTTGTCTT TGCGAGGAAG TTGCATTGGA CAAAGAGATG 
CAGTTCAGCA AACAGACCAT TGCGGCCATT TCGGAGCTGA CTTTCCGACA GTGTGAAAAT 
TTTGCCAAAG ACCTTGAAAT GTTTGCAAGA CATGCGAAAA GAACCACAAT TAACACTGAA 
GATGTGAAGC TCTTAGCCAG GAGGAGTAAT TCACTGCTAA AATACATCAC AGACAAAAGT 
GAAGAGATTG CTCAGATTAA CCTAGAACGA AAAGCACAGA AGAAAAAGAA GTCAGAGGAT 
GGAAGCAAAA ATTCAAGGCA GCCAGCAGAG GCTGGAGTGG TGGAAAGTGA GAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPS ( NM_199294.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_954988.1 Homo sapiens centromere protein S (CENPS), transcript variant A, mRNA.

Price & Availability↑

CloneID	OHu07808
Clone ID Related Accession (Same CDS sequence)	NM_199294.3 , NM_199294.2
Accession Version	NM_199294.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	centromere protein S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139424.21, BC029430.1, AL354956.13 and BE858209.1. On Jul 31, 2010 this sequence version replaced NM_199294.1. Transcript Variant: This variant (A) represents the shorter transcript but encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ433597.1, SRR1163658.189533.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGG AGGCGGAGAC CGAGGAGCAG CAGCGATTCT CTTACCAACA GAGGCTAAAG 
GCAGCAGTTC ACTATACTGT GGGTTGTCTT TGCGAGGAAG TTGCATTGGA CAAAGAGATG 
CAGTTCAGCA AACAGACCAT TGCGGCCATT TCGGAGCTGA CTTTCCGACA GTGTGAAAAT 
TTTGCCAAAG ACCTTGAAAT GTTTGCAAGA CATGCGAAAA GAACCACAAT TAACACTGAA 
GATGTGAAGC TCTTAGCCAG GAGGAGTAAT TCACTGCTAA AATACATCAC AGACAAAAGT 
GAAGAGATTG CTCAGATTAA CCTAGAACGA AAAGCACAGA AGAAAAAGAA GTCAGAGGAT 
GGAAGCAAAA ATTCAAGGCA GCCAGCAGAG GCTGGAGTGG TGGAAAGTGA GAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954988.1
CDS	417..833
Translation	MEEEAETEEQQRFSYQQRLKAAVHYTVGCLCEEVALDKEMQFSKQTIAAISELTFRQCENFAKDLEMFARHAKRTTINTEDVKLLARRSNSLLKYITDKSEEIAQINLERKAQKKKKSEDGSKNSRQPAEAGVVESEN

Target ORF information:

RefSeq Version	NM_199294.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centromere protein S (CENPS), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199294.2

ORF Insert Sequence:

ATGGAGGAGG AGGCGGAGAC CGAGGAGCAG CAGCGATTCT CTTACCAACA GAGGCTAAAG 
GCAGCAGTTC ACTATACTGT GGGTTGTCTT TGCGAGGAAG TTGCATTGGA CAAAGAGATG 
CAGTTCAGCA AACAGACCAT TGCGGCCATT TCGGAGCTGA CTTTCCGACA GTGTGAAAAT 
TTTGCCAAAG ACCTTGAAAT GTTTGCAAGA CATGCGAAAA GAACCACAAT TAACACTGAA 
GATGTGAAGC TCTTAGCCAG GAGGAGTAAT TCACTGCTAA AATACATCAC AGACAAAAGT 
GAAGAGATTG CTCAGATTAA CCTAGAACGA AAAGCACAGA AGAAAAAGAA GTCAGAGGAT 
GGAAGCAAAA ATTCAAGGCA GCCAGCAGAG GCTGGAGTGG TGGAAAGTGA GAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FANCM-associated proteins MHF1 and MHF2, but not the other Fanconi anemia factors, limit meiotic crossovers.
Nucleic acids research42(14)9087-95(2014 Aug)
Girard C,Crismani W,Froger N,Mazel J,Lemhemdi A,Horlow C,Mercier R

The histone-fold complex MHF is remodeled by FANCM to recognize branched DNA and protect genome stability.
Cell research24(5)560-75(2014 May)
Fox D 3rd,Yan Z,Ling C,Zhao Y,Lee DY,Fukagawa T,Yang W,Wang W

A CENP-S/X complex assembles at the centromere in S and G2 phases of the human cell cycle.
Open biology4130229(2014 Feb)
Dornblut C,Quinn N,Monajambashi S,Prendergast L,van Vuuren C,M?nch S,Deng W,Leonhardt H,Cardoso MC,Hoischen C,Diekmann S,Sullivan KF

The MHF complex senses branched DNA by binding a pair of crossover DNA duplexes.
Nature communications52987(2014)
Zhao Q,Saro D,Sachpatzidis A,Singh TR,Schlingman D,Zheng XF,Mack A,Tsai MS,Mochrie S,Regan L,Meetei AR,Sung P,Xiong Y

Emerging roles for centromere-associated proteins in DNA repair and genetic recombination.
Biochemical Society transactions41(6)1726-30(2013 Dec)
Osman F,Whitby MC

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CENPS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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