AQP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AQP2
Entrez Gene ID	359
Full Name	aquaporin 2
Synonyms	AQP-CD,WCH-CD
General protein information	Preferred Names aquaporin 2 Names aquaporin-2 ADH water channel AQP-2 aquaporin 2 (collecting duct) aquaporin-CD collecting duct water channel protein water channel protein for renal collecting duct water-channel aquaporin 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.12
Summary	This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008].
Disorder MIM:	107777
Disorder Html:	Diabetes insipidus, nephrogenic, 125800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000486.5	NP_000477.1	aquaporin-2
NM_000486.6	NP_000477.1	aquaporin-2

Pathways from BioSystems

Homologies

	AQP2	XP_001110572.1
Bos taurus (cattle)	AQP2	NP_001094669.1
Pan troglodytes (chimpanzee)	AQP2	XP_001157340.2
Rattus norvegicus (Norway rat)	Aqp2	NP_037041.2
Gallus gallus (chicken)	AQP2	XP_428855.4
Oryza sativa (rice)	Os04g0550950	NP_001174036.1
Oryza sativa (rice)	Os02g0658100	NP_001047632.1
Canis lupus familiaris (dog)	AQP2	XP_543678.2
Mus musculus (house mouse)	Aqp2	NP_033829.3
Homo sapiens (human)	AQP2	NP_000477.1
Arabidopsis thaliana (thale cress)	TIP2;2	NP_193465.1
Arabidopsis thaliana (thale cress)	TIP2;3	NP_199556.1
Xenopus tropicalis (tropical clawed frog)	aqp2	NP_001015749.1
Arabidopsis thaliana (thale cress)	DELTA-TIP	NP_188245.1

Gene Ontology
Bibliography

Related articles in PubMed

The 4.5 A structure of human AQP2.
Schenk AD, Werten PJ, Scheuring S, de Groot BL, M?ller SA, Stahlberg H, Philippsen A, Engel A
Journal of molecular biology350(2)278-89(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that AQP2 binds LIP5 in a AQP2-phosphorylation-dependent manner; phospho-mimicking mutations and phosphorylation reduce thermal stability of AQP2; AQP2 phosphorylation allosterically controls its interaction with LIP5. [AQP2 = aquaporin 2; LIP5 = LYST-interacting protein 5; LYST = lysosomal trafficking regulator protein]
Title: Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5.

It is a predictor for the development of renal insufficiency and death of patients with liver cirrhosis.
Title: Aquaporin-2 excretion in hospitalized patients with cirrhosis: Relation to development of renal insufficiency and mortality.

The present study provides detailed insights into the transport properties of AQP2 with the use of microsecond-scale molecular dynamics simulations, and explains how these channels conduct water molecules while at the same time excluding other molecules.
Title: Microsecond simulation of human aquaporin 2 reveals structural determinants of water permeability and selectivity.

aquaporin-2 regulates serine/threonine phosphatases in renal collecting duct
Title: Serine/threonine phosphatases and aquaporin-2 regulation in renal collecting duct.

Findings indicate that SIRT1 increases AQP2 expression in TNF-alpha-induced IMCD cells via the NF-kappaB-dependent signalling pathway, which might provide novel insight to understanding the renoprotective effects of SIRT1 in kidney diseases.
Title: Sirtuin1 (SIRT1) Regulates Tumor Necrosis Factor-alpha (TNF-?-Induced) Aquaporin-2 (AQP2) Expression in Renal Medullary Collecting Duct Cells Through Inhibiting the NF-?B Pathway.

The following AQP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AQP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu18847	NM_000486.5 Latest version!	Homo sapiens aquaporin 2 (AQP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu109362	NM_000486.6 Latest version!	Homo sapiens aquaporin 2 (AQP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AQP2 ( NM_000486.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000477.1 Homo sapiens aquaporin 2 (AQP2), mRNA.

Price & Availability↑

CloneID	OHu18847
Clone ID Related Accession (Same CDS sequence)	NM_000486.5
Accession Version	NM_000486.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	aquaporin-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK225940.1, AI792401.1, BC042496.1 and AC025154.31. This sequence is a reference standard in the RefSeqGene project. On Oct 9, 2008 this sequence version replaced NM_000486.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC042496.1, S73196.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGAGC TCCGCTCCAT AGCCTTCTCC AGGGCTGTGT TCGCAGAGTT CCTGGCCACA 
CTCCTCTTCG TCTTCTTTGG CCTCGGCTCT GCCCTCAACT GGCCACAGGC CCTGCCCTCT 
GTGCTACAGA TTGCCATGGC GTTTGGCTTG GGTATTGGCA CCCTGGTACA GGCTCTGGGC 
CACATAAGCG GGGCCCACAT CAACCCTGCC GTGACTGTGG CCTGCCTGGT GGGCTGCCAC 
GTCTCCGTTC TCCGAGCCGC CTTCTACGTG GCTGCCCAGC TGCTGGGGGC TGTGGCCGGA 
GCCGCTCTGC TCCATGAGAT CACGCCAGCA GACATCCGCG GGGACCTGGC TGTCAATGCT 
CTCAGCAACA GCACGACGGC TGGCCAGGCG GTGACTGTGG AGCTCTTCCT GACACTGCAG 
CTGGTGCTCT GCATCTTCGC CTCCACCGAT GAGCGCCGCG GAGAGAACCC GGGCACCCCT 
GCTCTCTCCA TAGGCTTCTC CGTGGCCCTG GGCCACCTCC TTGGGATCCA TTACACCGGC 
TGCTCTATGA ATCCTGCCCG CTCCCTGGCT CCAGCTGTCG TCACTGGCAA ATTTGATGAC 
CACTGGGTCT TCTGGATCGG ACCCCTGGTG GGCGCCATCC TGGGCTCCCT CCTCTACAAC 
TACGTGCTGT TTCCGCCAGC CAAGAGCCTG TCGGAGCGCC TGGCAGTGCT GAAGGGCCTG 
GAGCCGGACA CCGATTGGGA GGAGCGCGAG GTGCGACGGC GGCAGTCGGT GGAGCTGCAC 
TCGCCGCAGA GCCTGCCACG GGGTACCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000477.1
CDS	91..906
Translation	MWELRSIAFSRAVFAEFLATLLFVFFGLGSALNWPQALPSVLQIAMAFGLGIGTLVQALGHISGAHINPAVTVACLVGCHVSVLRAAFYVAAQLLGAVAGAALLHEITPADIRGDLAVNALSNSTTAGQAVTVELFLTLQLVLCIFASTDERRGENPGTPALSIGFSVALGHLLGIHYTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAILGSLLYNYVLFPPAKSLSERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGTKA

Target ORF information:

RefSeq Version	NM_000486.5
Organism	Homo sapiens(human)
Definition	Homo sapiens aquaporin 2 (AQP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000486.5

ORF Insert Sequence:

ATGTGGGAGC TCCGCTCCAT AGCCTTCTCC AGGGCTGTGT TCGCAGAGTT CCTGGCCACA 
CTCCTCTTCG TCTTCTTTGG CCTCGGCTCT GCCCTCAACT GGCCACAGGC CCTGCCCTCT 
GTGCTACAGA TTGCCATGGC GTTTGGCTTG GGTATTGGCA CCCTGGTACA GGCTCTGGGC 
CACATAAGCG GGGCCCACAT CAACCCTGCC GTGACTGTGG CCTGCCTGGT GGGCTGCCAC 
GTCTCCGTTC TCCGAGCCGC CTTCTACGTG GCTGCCCAGC TGCTGGGGGC TGTGGCCGGA 
GCCGCTCTGC TCCATGAGAT CACGCCAGCA GACATCCGCG GGGACCTGGC TGTCAATGCT 
CTCAGCAACA GCACGACGGC TGGCCAGGCG GTGACTGTGG AGCTCTTCCT GACACTGCAG 
CTGGTGCTCT GCATCTTCGC CTCCACCGAT GAGCGCCGCG GAGAGAACCC GGGCACCCCT 
GCTCTCTCCA TAGGCTTCTC CGTGGCCCTG GGCCACCTCC TTGGGATCCA TTACACCGGC 
TGCTCTATGA ATCCTGCCCG CTCCCTGGCT CCAGCTGTCG TCACTGGCAA ATTTGATGAC 
CACTGGGTCT TCTGGATCGG ACCCCTGGTG GGCGCCATCC TGGGCTCCCT CCTCTACAAC 
TACGTGCTGT TTCCGCCAGC CAAGAGCCTG TCGGAGCGCC TGGCAGTGCT GAAGGGCCTG 
GAGCCGGACA CCGATTGGGA GGAGCGCGAG GTGCGACGGC GGCAGTCGGT GGAGCTGCAC 
TCGCCGCAGA GCCTGCCACG GGGTACCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AQP2 ( NM_000486.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_000477.1 Homo sapiens aquaporin 2 (AQP2), mRNA.

Price & Availability↑

CloneID	OHu109362
Clone ID Related Accession (Same CDS sequence)	NM_000486.6
Accession Version	NM_000486.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	aquaporin-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025154.31, AK225940.1, AI792401.1 and BC042496.1. On Dec 27, 2019 this sequence version replaced NM_000486.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC042496.1, S73196.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGAGC TCCGCTCCAT AGCCTTCTCC AGGGCTGTGT TCGCAGAGTT CCTGGCCACA 
CTCCTCTTCG TCTTCTTTGG CCTCGGCTCT GCCCTCAACT GGCCACAGGC CCTGCCCTCT 
GTGCTACAGA TTGCCATGGC GTTTGGCTTG GGTATTGGCA CCCTGGTACA GGCTCTGGGC 
CACATAAGCG GGGCCCACAT CAACCCTGCC GTGACTGTGG CCTGCCTGGT GGGCTGCCAC 
GTCTCCGTTC TCCGAGCCGC CTTCTACGTG GCTGCCCAGC TGCTGGGGGC TGTGGCCGGA 
GCCGCTCTGC TCCATGAGAT CACGCCAGCA GACATCCGCG GGGACCTGGC TGTCAATGCT 
CTCAGCAACA GCACGACGGC TGGCCAGGCG GTGACTGTGG AGCTCTTCCT GACACTGCAG 
CTGGTGCTCT GCATCTTCGC CTCCACCGAT GAGCGCCGCG GAGAGAACCC GGGCACCCCT 
GCTCTCTCCA TAGGCTTCTC TGTGGCCCTG GGCCACCTCC TTGGGATCCA TTACACCGGC 
TGCTCTATGA ATCCTGCCCG CTCCCTGGCT CCAGCTGTCG TCACTGGCAA ATTTGATGAC 
CACTGGGTCT TCTGGATCGG ACCCCTGGTG GGCGCCATCC TGGGCTCCCT CCTCTACAAC 
TACGTGCTGT TTCCGCCAGC CAAGAGCCTG TCGGAGCGCC TGGCAGTGCT GAAGGGCCTG 
GAGCCGGACA CCGATTGGGA GGAGCGCGAG GTGCGACGGC GGCAGTCGGT GGAGCTGCAC 
TCGCCGCAGA GCCTGCCACG GGGTACCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000477.1
CDS	95..910
Translation	MWELRSIAFSRAVFAEFLATLLFVFFGLGSALNWPQALPSVLQIAMAFGLGIGTLVQALGHISGAHINPAVTVACLVGCHVSVLRAAFYVAAQLLGAVAGAALLHEITPADIRGDLAVNALSNSTTAGQAVTVELFLTLQLVLCIFASTDERRGENPGTPALSIGFSVALGHLLGIHYTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAILGSLLYNYVLFPPAKSLSERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGTKA

Target ORF information:

RefSeq Version	NM_000486.6
Organism	Homo sapiens(human)
Definition	Homo sapiens aquaporin 2 (AQP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000486.6

ORF Insert Sequence:

ATGTGGGAGC TCCGCTCCAT AGCCTTCTCC AGGGCTGTGT TCGCAGAGTT CCTGGCCACA 
CTCCTCTTCG TCTTCTTTGG CCTCGGCTCT GCCCTCAACT GGCCACAGGC CCTGCCCTCT 
GTGCTACAGA TTGCCATGGC GTTTGGCTTG GGTATTGGCA CCCTGGTACA GGCTCTGGGC 
CACATAAGCG GGGCCCACAT CAACCCTGCC GTGACTGTGG CCTGCCTGGT GGGCTGCCAC 
GTCTCCGTTC TCCGAGCCGC CTTCTACGTG GCTGCCCAGC TGCTGGGGGC TGTGGCCGGA 
GCCGCTCTGC TCCATGAGAT CACGCCAGCA GACATCCGCG GGGACCTGGC TGTCAATGCT 
CTCAGCAACA GCACGACGGC TGGCCAGGCG GTGACTGTGG AGCTCTTCCT GACACTGCAG 
CTGGTGCTCT GCATCTTCGC CTCCACCGAT GAGCGCCGCG GAGAGAACCC GGGCACCCCT 
GCTCTCTCCA TAGGCTTCTC TGTGGCCCTG GGCCACCTCC TTGGGATCCA TTACACCGGC 
TGCTCTATGA ATCCTGCCCG CTCCCTGGCT CCAGCTGTCG TCACTGGCAA ATTTGATGAC 
CACTGGGTCT TCTGGATCGG ACCCCTGGTG GGCGCCATCC TGGGCTCCCT CCTCTACAAC 
TACGTGCTGT TTCCGCCAGC CAAGAGCCTG TCGGAGCGCC TGGCAGTGCT GAAGGGCCTG 
GAGCCGGACA CCGATTGGGA GGAGCGCGAG GTGCGACGGC GGCAGTCGGT GGAGCTGCAC 
TCGCCGCAGA GCCTGCCACG GGGTACCAAG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The 4.5 A structure of human AQP2.
Journal of molecular biology350(2)278-89(2005 Jul)
Schenk AD,Werten PJ,Scheuring S,de Groot BL,M?ller SA,Stahlberg H,Philippsen A,Engel A

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AQP2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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