DELTA-TIP cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DELTA-TIP
Entrez Gene ID	820870
Full Name	delta tonoplast integral protein
Synonyms	AQP1,ATTIP2;1,DELTA-TIP1,TIP2;1,delta tonoplast integral protein
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_112495.4	NP_188245.1	delta tonoplast integral protein

Pathways from BioSystems

Homologies

	AQP2	XP_001110572.1
Bos taurus (cattle)	AQP2	NP_001094669.1
Pan troglodytes (chimpanzee)	AQP2	XP_001157340.2
Rattus norvegicus (Norway rat)	Aqp2	NP_037041.2
Gallus gallus (chicken)	AQP2	XP_428855.4
Oryza sativa (rice)	Os04g0550950	NP_001174036.1
Oryza sativa (rice)	Os02g0658100	NP_001047632.1
Canis lupus familiaris (dog)	AQP2	XP_543678.2
Mus musculus (house mouse)	Aqp2	NP_033829.3
Homo sapiens (human)	AQP2	NP_000477.1
Arabidopsis thaliana (thale cress)	TIP2;2	NP_193465.1
Arabidopsis thaliana (thale cress)	TIP2;3	NP_199556.1
Xenopus tropicalis (tropical clawed frog)	aqp2	NP_001015749.1
Arabidopsis thaliana (thale cress)	DELTA-TIP	NP_188245.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The relatively wide pore and the polar nature of the selectivity filter clarify the ammonia permeability.
Title: Crystal Structure of an Ammonia-Permeable Aquaporin.

Data show that the highly abundant tonoplast aquaporins (AQPs) isoforms AtTIP1;1, AtTIP1;2, and AtTIP2;1 facilitate the emergence of new lateral root primordia (LRPs).
Title: Tonoplast Aquaporins Facilitate Lateral Root Emergence.

unlike for TIP1;1, trafficking of markers for TIP3;1 and TIP2;1 is insensitive to Brefeldin A in Arabidopsis hypocotyls
Title: A small molecule inhibitor partitions two distinct pathways for trafficking of tonoplast intrinsic proteins in Arabidopsis.

AtTIP2;1 and AtTIP2;3 can mediate the extracytosolic transport of methyl-NH2 and NH3 across the tonoplast membrane and may thus participate in vacuolar ammonium compartmentation. [TIP2;1]
Title: Tonoplast intrinsic proteins AtTIP2;1 and AtTIP2;3 facilitate NH3 transport into the vacuole.

The following DELTA-TIP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DELTA-TIP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb11322	NM_112495.4 Latest version!	Arabidopsis thaliana delta tonoplast integral protein (DELTA-TIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DELTA-TIP ( NM_112495.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_188245.1 Arabidopsis thaliana delta tonoplast integral protein (DELTA-TIP), mRNA.

Price & Availability↑

CloneID	OAb11322
Clone ID Related Accession (Same CDS sequence)	NM_112495.4
Accession Version	NM_112495.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	delta tonoplast integral protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_112495.3.

ATGGCTGGAG TTGCCTTTGG TTCCTTTGAT GATTCATTCA GCTTGGCTTC TCTAAGAGCT 
TACCTCGCTG AGTTCATCTC CACTTTACTC TTTGTTTTCG CTGGTGTTGG CTCTGCCATT 
GCCTACGCAA AGCTGACGTC GGACGCTGCT CTTGATACAC CGGGACTAGT GGCCATCGCG 
GTTTGTCATG GTTTTGCTCT CTTCGTGGCC GTTGCAATCG GAGCCAACAT CTCCGGTGGC 
CATGTGAACC CAGCCGTCAC TTTTGGTCTT GCTGTCGGTG GTCAAATCAC AGTCATCACC 
GGAGTTTTCT ACTGGATCGC TCAGCTTCTC GGCTCCACCG CCGCTTGTTT CCTCCTTAAA 
TACGTCACCG GTGGATTGGC GGTTCCAACC CACAGCGTTG CGGCTGGACT AGGATCGATA 
GAAGGAGTAG TGATGGAGAT CATCATCACC TTCGCTTTGG TCTACACTGT CTACGCCACC 
GCCGCTGATC CCAAGAAGGG TTCTCTCGGA ACCATCGCTC CTCTCGCCAT TGGTCTTATC 
GTTGGTGCCA ACATCCTCGC CGCCGGTCCA TTCTCCGGTG GATCCATGAA CCCAGCACGT 
TCCTTTGGAC CAGCTGTTGC TGCCGGAGAC TTCTCTGGTC ACTGGGTCTA CTGGGTTGGA 
CCACTCATCG GTGGTGGACT TGCCGGACTT ATCTACGGAA ATGTCTTCAT GGGTTCTTCC 
GAACATGTTC CTCTTGCTTC TGCTGATTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_188245.1
CDS	175..927
Translation	MAGVAFGSFDDSFSLASLRAYLAEFISTLLFVFAGVGSAIAYAKLTSDAALDTPGLVAIAVCHGFALFVAVAIGANISGGHVNPAVTFGLAVGGQITVITGVFYWIAQLLGSTAACFLLKYVTGGLAVPTHSVAAGLGSIEGVVMEIIITFALVYTVYATAADPKKGSLGTIAPLAIGLIVGANILAAGPFSGGSMNPARSFGPAVAAGDFSGHWVYWVGPLIGGGLAGLIYGNVFMGSSEHVPLASADF

Target ORF information:

RefSeq Version	NM_112495.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana delta tonoplast integral protein (DELTA-TIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_112495.4

ORF Insert Sequence:

ATGGCTGGAG TTGCCTTTGG TTCCTTTGAT GATTCATTCA GCTTGGCTTC TCTAAGAGCT 
TACCTCGCTG AGTTCATCTC CACTTTACTC TTTGTTTTCG CTGGTGTTGG CTCTGCCATT 
GCCTACGCAA AGCTGACGTC GGACGCTGCT CTTGATACAC CGGGACTAGT GGCCATCGCG 
GTTTGTCATG GTTTTGCTCT CTTCGTGGCC GTTGCAATCG GAGCCAACAT CTCCGGTGGC 
CATGTGAACC CAGCCGTCAC TTTTGGTCTT GCTGTCGGTG GTCAAATCAC AGTCATCACC 
GGAGTTTTCT ACTGGATCGC TCAGCTTCTC GGCTCCACCG CCGCTTGTTT CCTCCTTAAA 
TACGTCACCG GTGGATTGGC GGTTCCAACC CACAGCGTTG CGGCTGGACT AGGATCGATA 
GAAGGAGTAG TGATGGAGAT CATCATCACC TTCGCTTTGG TCTACACTGT CTACGCCACC 
GCCGCTGATC CCAAGAAGGG TTCTCTCGGA ACCATCGCTC CTCTCGCCAT TGGTCTTATC 
GTTGGTGCCA ACATCCTCGC CGCCGGTCCA TTCTCCGGTG GATCCATGAA CCCAGCACGT 
TCCTTTGGAC CAGCTGTTGC TGCCGGAGAC TTCTCTGGTC ACTGGGTCTA CTGGGTTGGA 
CCACTCATCG GTGGTGGACT TGCCGGACTT ATCTACGGAA ATGTCTTCAT GGGTTCTTCC 
GAACATGTTC CTCTTGCTTC TGCTGATTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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DELTA-TIP cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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