AMIGO2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AMIGO2
Entrez Gene ID	347902
Full Name	adhesion molecule with Ig like domain 2
Synonyms	ALI1,AMIGO-2,DEGA
General protein information	Preferred Names adhesion molecule with Ig like domain 2 Names amphoterin-induced protein 2 alivin 1 amphoterin induced gene 2 differentially expressed in gastric adenocarcinoma differentially expressed in gastric adenocarcinomas transmembrane protein AMIGO2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.11
Disorder MIM:	615690

mRNA and Protein(s)

mRNA	Protein	Name
XM_005268837.2	XP_005268894.1	amphoterin-induced protein 2 isoform X1
XM_005268837.3	XP_005268894.1	amphoterin-induced protein 2 isoform X1
NM_001143668.1	NP_001137140.1	amphoterin-induced protein 2 precursor
NM_001370299.1	NP_001357228.1	amphoterin-induced protein 2 precursor
NM_181847.4	NP_862830.1	amphoterin-induced protein 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AMIGO2	XP_005636999.1
Xenopus tropicalis (tropical clawed frog)	amigo2	XP_002932865.1
Homo sapiens (human)	AMIGO2	NP_001137140.1
Bos taurus (cattle)	AMIGO2	NP_001192715.1
Mus musculus (house mouse)	Amigo2	NP_001158035.1
Gallus gallus (chicken)	AMIGO2	NP_001186479.1
Pan troglodytes (chimpanzee)	AMIGO2	XP_001163356.1
	AMIGO2	XP_001093749.2
Rattus norvegicus (Norway rat)	Amigo2	NP_877968.2

Gene Ontology
Bibliography

Related articles in PubMed

Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene.
Fontanals-Cirera B, Hasson D, Vardabasso C, Di Micco R, Agrawal P, Chowdhury A, Gantz M, de Pablos-Aragoneses A, Morgenstern A, Wu P, Filipescu D, Valle-Garcia D, Darvishian F, Roe JS, Davies MA, Vakoc CR, Hernando E, Bernstein E
Molecular cell68(4)731-744.e9(2017 Nov)

Amigo2-upregulation in Tumour Cells Facilitates Their Attachment to Liver Endothelial Cells Resulting in Liver Metastases.
Kanda Y, Osaki M, Onuma K, Sonoda A, Kobayashi M, Hamada J, Nicolson GL, Ochiya T, Okada F
Scientific reports743567(2017 Mar)

AMIGO2, a novel membrane anchor of PDK1, controls cell survival and angiogenesis via Akt activation.
Park H, Lee S, Shrestha P, Kim J, Park JA, Ko Y, Ban YH, Park DY, Ha SJ, Koh GY, Hong VS, Mochizuki N, Kim YM, Lee W, Kwon YG
The Journal of cell biology211(3)619-37(2015 Nov)

A genome-wide search for loci interacting with known prostate cancer risk-associated genetic variants.
Tao S, Wang Z, Feng J, Hsu FC, Jin G, Kim ST, Zhang Z, Gronberg H, Zheng LS, Isaacs WB, Xu J, Sun J
Carcinogenesis33(3)598-603(2012 Mar)

Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.
Kahle JJ, Gulbahce N, Shaw CA, Lim J, Hill DE, Barab?si AL, Zoghbi HY
Human molecular genetics20(3)510-27(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study uncovers mechanisms underlying the therapeutic effects of BETi in melanoma and reveals the AMIGO2-PTK7 axis as a targetable pathway for metastatic melanoma.
Title: Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene.

AMIGO2 is an important regulator of the PDK1-Akt pathway.
Title: AMIGO2, a novel membrane anchor of PDK1, controls cell survival and angiogenesis via Akt activation.

Stable expression of a DEGA/AMIGO-2 antisense construct led to altered morphology, increased ploidy, chromosomal instability, decreased cell adhesion/migration, and a nearly complete abrogation of tumorigenicity in nude mice.
Title: DEGA/AMIGO-2, a leucine-rich repeat family member, differentially expressed in human gastric adenocarcinoma: effects on ploidy, chromosomal stability, cell adhesion/migration and tumorigenicity.

Expression of ali1 promotes depolarization-dependent survival of cerebellar granule neurons. Mouse ali1 mapped to a chromosome 15 locus syntenic to candidate loci for familial Alzheimer's disease type 5 and Parkinson's disease 8 on human chromosome 12.
Title: Alivin 1, a novel neuronal activity-dependent gene, inhibits apoptosis and promotes survival of cerebellar granule neurons.

The following AMIGO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMIGO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09903	NM_001143668.1 Latest version!	Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09903	NM_001370299.1 Latest version!	Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09903	NM_181847.4 Latest version!	Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu09903	XM_005268837.3 Latest version!	Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu09903	XM_005268837.2	Homo sapiens adhesion molecule with Ig-like domain 2 (AMIGO2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMIGO2 ( NM_001143668.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137140.1 Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09903
Clone ID Related Accession (Same CDS sequence)	XM_005268837.2 , XM_005268837.3 , NM_001143668.1 , NM_001370299.1 , NM_181847.4
Accession Version	NM_001143668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	amphoterin-induced protein 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC095477.1, AC004010.1, AB079074.1 and BC047595.1. Transcript Variant: This variant (1) includes an alternate exon in the 5' UTR. The same protein is encoded by transcript variants 1-3. ##Evidence-Data-START## Transcript exon combination :: BC095477.1, SRR1803615.222803.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137140.1
CDS	656..2224
Translation	MSLRVHTLPTLLGAVVRPGCRELLCLLMITVTVGPGASGVCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHEAFNTAFTTLAACVASIVLVLLYLYLTPCPCKCKTKRQKNMLHQSNAHSSILSPGPASDASADERKAGAGKRVVFLEPLKDTAAGQNGKVRLFPSEAVIAEGILKSTRGKSDSDSVNSVFSDTPFVAST

Target ORF information:

RefSeq Version	NM_001143668.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143668.1

ORF Insert Sequence:

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMIGO2 ( NM_001370299.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357228.1 Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09903
Clone ID Related Accession (Same CDS sequence)	XM_005268837.2 , XM_005268837.3 , NM_001143668.1 , NM_001370299.1 , NM_181847.4
Accession Version	NM_001370299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	amphoterin-induced protein 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004010.1. On May 4, 2019 this sequence version replaced XM_005268837.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DRR138514.18657.1, AL833007.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357228.1
CDS	669..2237
Translation	MSLRVHTLPTLLGAVVRPGCRELLCLLMITVTVGPGASGVCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHEAFNTAFTTLAACVASIVLVLLYLYLTPCPCKCKTKRQKNMLHQSNAHSSILSPGPASDASADERKAGAGKRVVFLEPLKDTAAGQNGKVRLFPSEAVIAEGILKSTRGKSDSDSVNSVFSDTPFVAST

Target ORF information:

RefSeq Version	NM_001370299.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370299.1

ORF Insert Sequence:

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMIGO2 ( NM_181847.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_862830.1 Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09903
Clone ID Related Accession (Same CDS sequence)	XM_005268837.2 , XM_005268837.3 , NM_001143668.1 , NM_001370299.1 , NM_181847.4
Accession Version	NM_181847.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	amphoterin-induced protein 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC095477.1, AC004010.1, CN397638.1, AY454159.1 and AB079074.1. On Jan 3, 2009 this sequence version replaced NM_181847.3. Transcript Variant: This variant (2) omits an alternate exon from the 5' UTR. The same protein is encoded by transcript variants 1-3. ##Evidence-Data-START## Transcript exon combination :: AY454159.1, BC047595.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_862830.1
CDS	554..2122
Translation	MSLRVHTLPTLLGAVVRPGCRELLCLLMITVTVGPGASGVCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHEAFNTAFTTLAACVASIVLVLLYLYLTPCPCKCKTKRQKNMLHQSNAHSSILSPGPASDASADERKAGAGKRVVFLEPLKDTAAGQNGKVRLFPSEAVIAEGILKSTRGKSDSDSVNSVFSDTPFVAST

Target ORF information:

RefSeq Version	NM_181847.4
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181847.4

ORF Insert Sequence:

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMIGO2 ( XM_005268837.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268894.1 Homo sapiens adhesion molecule with Ig-like domain 2 (AMIGO2), transcript variant X1, mRNA.

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CloneID	OHu09903
Clone ID Related Accession (Same CDS sequence)	XM_005268837.2 , XM_005268837.3 , NM_001143668.1 , NM_001370299.1 , NM_181847.4
Accession Version	XM_005268837.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	amphoterin-induced protein 2 isoform X1
Comment

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005268894.1

Translation

MSLRVHTLPTLLGAVVRPGCRELLCLLMITVTVGPGASGVCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHEAFNTAFTTLAACVASIVLVLLYLYLTPCPCKCKTKRQKNMLHQSNAHSSILSPGPASDASADERKAGAGKRVVFLEPLKDTAAGQNGKVRLFPSEAVIAEGILKSTRGKSDSDSVNSVFSDTPFVAST

Target ORF information:

RefSeq Version	XM_005268837.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens adhesion molecule with Ig-like domain 2 (AMIGO2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268837.2

ORF Insert Sequence:

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMIGO2 ( XM_005268837.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268894.1 Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu09903
Clone ID Related Accession (Same CDS sequence)	XM_005268837.2 , XM_005268837.3 , NM_001143668.1 , NM_001370299.1 , NM_181847.4
Accession Version	XM_005268837.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	amphoterin-induced protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268837.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268894.1
CDS	1536..3104
Translation	MSLRVHTLPTLLGAVVRPGCRELLCLLMITVTVGPGASGVCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHEAFNTAFTTLAACVASIVLVLLYLYLTPCPCKCKTKRQKNMLHQSNAHSSILSPGPASDASADERKAGAGKRVVFLEPLKDTAAGQNGKVRLFPSEAVIAEGILKSTRGKSDSDSVNSVFSDTPFVAST

Target ORF information:

RefSeq Version	XM_005268837.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adhesion molecule with Ig like domain 2 (AMIGO2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268837.3

ORF Insert Sequence:

ATGTCGTTAC GTGTACACAC TCTGCCCACC CTGCTTGGAG CCGTCGTCAG ACCGGGCTGC 
AGGGAGCTGC TGTGTTTGCT GATGATCACA GTGACTGTGG GCCCTGGTGC CTCTGGGGTG 
TGCCCCACCG CTTGCATCTG TGCCACTGAC ATCGTCAGCT GCACCAACAA AAACCTGTCC 
AAGGTGCCTG GGAACCTTTT CAGACTGATT AAGAGACTGG ACCTGAGTTA TAACAGAATT 
GGGCTTCTGG ATTCTGAGTG GATTCCAGTA TCGTTTGCAA AGCTGAACAC CCTAATTCTT 
CGTCATAACA ACATCACCAG CATTTCCACG GGCAGTTTTT CCACAACTCC AAATTTGAAG 
TGTCTTGACT TATCGTCCAA TAAGCTGAAG ACGGTGAAAA ATGCTGTATT CCAAGAGTTG 
AAGGTTCTGG AAGTGCTTCT GCTTTACAAC AATCACATAT CCTATCTCGA TCCTTCAGCG 
TTTGGAGGGC TCTCCCAGTT GCAGAAACTC TACTTAAGTG GAAATTTTCT CACACAGTTT 
CCGATGGATT TGTATGTTGG AAGGTTCAAG CTGGCAGAAC TGATGTTTTT AGATGTTTCT 
TATAACCGAA TTCCTTCCAT GCCAATGCAC CACATAAATT TAGTGCCAGG AAAACAGCTG 
AGAGGCATCT ACCTTCATGG AAACCCATTT GTCTGTGACT GTTCCCTGTA CTCCTTGCTG 
GTCTTTTGGT ATCGTAGGCA CTTTAGCTCA GTGATGGATT TTAAGAACGA TTACACCTGT 
CGCCTGTGGT CTGACTCCAG GCACTCGCGT CAGGTACTTC TGCTCCAGGA TAGCTTTATG 
AATTGCTCTG ACAGCATCAT CAATGGTTCC TTTCGTGCGC TTGGCTTTAT TCATGAGGCT 
CAGGTCGGGG AAAGACTGAT GGTCCACTGT GACAGCAAGA CAGGTAATGC AAATACGGAT 
TTCATCTGGG TGGGTCCAGA TAACAGACTG CTAGAGCCGG ATAAAGAGAT GGAAAACTTT 
TACGTGTTTC ACAATGGAAG TCTGGTTATA GAAAGCCCTC GTTTTGAGGA TGCTGGAGTG 
TATTCTTGTA TCGCAATGAA TAAGCAACGC CTGTTAAATG AAACTGTGGA CGTCACAATA 
AATGTGAGCA ATTTCACTGT AAGCAGATCC CATGCTCATG AGGCATTTAA CACAGCTTTT 
ACCACTCTTG CTGCTTGCGT GGCCAGTATC GTTTTGGTAC TTTTGTACCT CTATCTGACT 
CCATGCCCCT GCAAGTGTAA AACCAAGAGA CAGAAAAATA TGCTACACCA AAGCAATGCC 
CATTCATCGA TTCTCAGTCC TGGCCCCGCT AGTGATGCCT CCGCTGATGA ACGGAAGGCA 
GGTGCAGGTA AAAGAGTGGT GTTTTTGGAA CCCCTGAAGG ATACTGCAGC AGGGCAGAAC 
GGGAAAGTCA GGCTCTTTCC CAGCGAGGCA GTGATAGCTG AGGGCATCCT AAAGTCCACG 
AGGGGGAAAT CTGACTCAGA TTCAGTCAAT TCAGTGTTTT CTGACACACC TTTTGTGGCG 
TCCACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene.
Molecular cell68(4)731-744.e9(2017 Nov)
Fontanals-Cirera B,Hasson D,Vardabasso C,Di Micco R,Agrawal P,Chowdhury A,Gantz M,de Pablos-Aragoneses A,Morgenstern A,Wu P,Filipescu D,Valle-Garcia D,Darvishian F,Roe JS,Davies MA,Vakoc CR,Hernando E,Bernstein E

Amigo2-upregulation in Tumour Cells Facilitates Their Attachment to Liver Endothelial Cells Resulting in Liver Metastases.
Scientific reports743567(2017 Mar)
Kanda Y,Osaki M,Onuma K,Sonoda A,Kobayashi M,Hamada J,Nicolson GL,Ochiya T,Okada F

AMIGO2, a novel membrane anchor of PDK1, controls cell survival and angiogenesis via Akt activation.
The Journal of cell biology211(3)619-37(2015 Nov)
Park H,Lee S,Shrestha P,Kim J,Park JA,Ko Y,Ban YH,Park DY,Ha SJ,Koh GY,Hong VS,Mochizuki N,Kim YM,Lee W,Kwon YG

A genome-wide search for loci interacting with known prostate cancer risk-associated genetic variants.
Carcinogenesis33(3)598-603(2012 Mar)
Tao S,Wang Z,Feng J,Hsu FC,Jin G,Kim ST,Zhang Z,Gronberg H,Zheng LS,Isaacs WB,Xu J,Sun J

Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.
Human molecular genetics20(3)510-27(2011 Feb)
Kahle JJ,Gulbahce N,Shaw CA,Lim J,Hill DE,Barab?si AL,Zoghbi HY

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AMIGO2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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