DPPA5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DPPA5
Entrez Gene ID	340168
Full Name	developmental pluripotency associated 5
Synonyms	ESG1
General protein information	Preferred Names developmental pluripotency associated 5 Names developmental pluripotency-associated 5 protein embryonal stem cell specific gene 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q13
Summary	This gene encodes a protein that may function in the control of cell pluripotency and early embryogenesis. Expression of this gene is a specific marker for pluripotent stem cells. Pseudogenes of this gene are located on the short arm of chromosome 10 and the long arm of chromosomes 14 and 19. [provided by RefSeq, Dec 2010].
Disorder MIM:	611111

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025290.3	NP_001020461.1	developmental pluripotency-associated 5 protein
NM_001025290.2	NP_001020461.1	developmental pluripotency-associated 5 protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DPPA5	XP_003311448.1
Homo sapiens (human)	DPPA5	NP_001020461.1
	LOC715759	XP_001112340.1
Canis lupus familiaris (dog)	DPPA5	XP_003639485.1
Mus musculus (house mouse)	Dppa5a	NP_079550.2

Gene Ontology
Bibliography

Related articles in PubMed

DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover.
Qian X, Kim JK, Tong W, Villa-Diaz LG, Krebsbach PH
Stem cells (Dayton, Ohio)34(3)588-600(2016 Mar)

Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC.
Aoki T, Ohnishi H, Oda Y, Tadokoro M, Sasao M, Kato H, Hattori K, Ohgushi H
Tissue engineering. Part A16(7)2197-206(2010 Jul)

Gene birth, death, and divergence: the different scenarios of reproduction-related gene evolution.
Tian X, Pascal G, Fouch?court S, Pontarotti P, Monget P
Biology of reproduction80(4)616-21(2009 Apr)

The role of promoter CpG methylation in the epigenetic control of stem cell related genes during differentiation.
Dansranjavin T, Krehl S, Mueller T, Mueller LP, Schmoll HJ, Dammann RH
Cell cycle (Georgetown, Tex.)8(6)916-24(2009 Mar)

Genome-wide profiling of DNA methylation reveals a class of normally methylated CpG island promoters.
Shen L, Kondo Y, Guo Y, Zhang J, Zhang L, Ahmed S, Shu J, Chen X, Waterland RA, Issa JP
PLoS genetics3(10)2023-36(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our study provides new insight into the function of DPPA5 and NANOG regulation in human pluripotent stem cell .
Title: DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover.

Dppa5 is specifically and differentially expressed in human cells that have pluripotency and can be used as a marker of pluripotent stem cells.
Title: Identification of developmental pluripotency associated 5 expression in human pluripotent stem cells.

The following DPPA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPPA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04356	NM_001025290.3 Latest version!	Homo sapiens developmental pluripotency associated 5 (DPPA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu04356	NM_001025290.2	Homo sapiens developmental pluripotency associated 5 (DPPA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPPA5 ( NM_001025290.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020461.1 Homo sapiens developmental pluripotency associated 5 (DPPA5), mRNA.

Price & Availability↑

CloneID	OHu04356
Clone ID Related Accession (Same CDS sequence)	NM_001025290.3 , NM_001025290.2
Accession Version	NM_001025290.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated 5 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC137549.1 and AI365263.1. On Nov 22, 2018 this sequence version replaced NM_001025290.2. ##Evidence-Data-START## Transcript exon combination :: BX092581.1, BC137549.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370370.4/ ENSP00000359396.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAACTC TCCCGGCACG TAGACATATC CCGCCGTGGG TGAAAGTTCC CGAAGACCTG 
AAAGATCCAG AGGTGTTCCA GGTCCAGACG CGGCTGCTGA AAGCCATTTT CGGCCCGGAC 
GGATCTCGAA TCCCTTACAT CGAGCAGGTG AGCAAGGCCA TGCTCGAGCT GAAGGCTCTG 
GAGTCTTCAG ACCTCACCGA GGTCGTGGTT TACGGCTCCT ATTTGTACAA GCTCCGGACC 
AAGTGGATGC TCCAGTCCAT GGCTGAGTGG CACCGCCAGC GCCAGGAGCG AGGGATGCTC 
AAACTTGCCG AAGCCATGAA TGCCCTCGAA CTAGGCCCTT GGATGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020461.1
CDS	52..402
Translation	MGTLPARRHIPPWVKVPEDLKDPEVFQVQTRLLKAIFGPDGSRIPYIEQVSKAMLELKALESSDLTEVVVYGSYLYKLRTKWMLQSMAEWHRQRQERGMLKLAEAMNALELGPWMK

Target ORF information:

RefSeq Version	NM_001025290.3
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 5 (DPPA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025290.3

ORF Insert Sequence:

ATGGGAACTC TCCCGGCACG TAGACATATC CCGCCGTGGG TGAAAGTTCC CGAAGACCTG 
AAAGATCCAG AGGTGTTCCA GGTCCAGACG CGGCTGCTGA AAGCCATTTT CGGCCCGGAC 
GGATCTCGAA TCCCTTACAT CGAGCAGGTG AGCAAGGCCA TGCTCGAGCT GAAGGCTCTG 
GAGTCTTCAG ACCTCACCGA GGTCGTGGTT TACGGCTCCT ATTTGTACAA GCTCCGGACC 
AAGTGGATGC TCCAGTCCAT GGCTGAGTGG CACCGCCAGC GCCAGGAGCG AGGGATGCTC 
AAACTTGCCG AAGCCATGAA TGCCCTCGAA CTAGGCCCTT GGATGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPPA5 ( NM_001025290.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020461.1 Homo sapiens developmental pluripotency associated 5 (DPPA5), mRNA.

Price & Availability↑

CloneID	OHu04356
Clone ID Related Accession (Same CDS sequence)	NM_001025290.3 , NM_001025290.2
Accession Version	NM_001025290.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	developmental pluripotency-associated 5 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC137549.1 and AI365263.1. On Dec 15, 2010 this sequence version replaced NM_001025290.1. ##Evidence-Data-START## Transcript exon combination :: BC137549.1, BX092581.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGGGAACTC TCCCGGCACG TAGACATATC CCGCCGTGGG TGAAAGTTCC CGAAGACCTG 
AAAGATCCAG AGGTGTTCCA GGTCCAGACG CGGCTGCTGA AAGCCATTTT CGGCCCGGAC 
GGATCTCGAA TCCCTTACAT CGAGCAGGTG AGCAAGGCCA TGCTCGAGCT GAAGGCTCTG 
GAGTCTTCAG ACCTCACCGA GGTCGTGGTT TACGGCTCCT ATTTGTACAA GCTCCGGACC 
AAGTGGATGC TCCAGTCCAT GGCTGAGTGG CACCGCCAGC GCCAGGAGCG AGGGATGCTC 
AAACTTGCCG AAGCCATGAA TGCCCTCGAA CTAGGCCCTT GGATGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020461.1
CDS	52..402
Translation	MGTLPARRHIPPWVKVPEDLKDPEVFQVQTRLLKAIFGPDGSRIPYIEQVSKAMLELKALESSDLTEVVVYGSYLYKLRTKWMLQSMAEWHRQRQERGMLKLAEAMNALELGPWMK

Target ORF information:

RefSeq Version	NM_001025290.2
Organism	Homo sapiens(human)
Definition	Homo sapiens developmental pluripotency associated 5 (DPPA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025290.2

ORF Insert Sequence:

ATGGGAACTC TCCCGGCACG TAGACATATC CCGCCGTGGG TGAAAGTTCC CGAAGACCTG 
AAAGATCCAG AGGTGTTCCA GGTCCAGACG CGGCTGCTGA AAGCCATTTT CGGCCCGGAC 
GGATCTCGAA TCCCTTACAT CGAGCAGGTG AGCAAGGCCA TGCTCGAGCT GAAGGCTCTG 
GAGTCTTCAG ACCTCACCGA GGTCGTGGTT TACGGCTCCT ATTTGTACAA GCTCCGGACC 
AAGTGGATGC TCCAGTCCAT GGCTGAGTGG CACCGCCAGC GCCAGGAGCG AGGGATGCTC 
AAACTTGCCG AAGCCATGAA TGCCCTCGAA CTAGGCCCTT GGATGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover.
Stem cells (Dayton, Ohio)34(3)588-600(2016 Mar)
Qian X,Kim JK,Tong W,Villa-Diaz LG,Krebsbach PH

Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC.
Tissue engineering. Part A16(7)2197-206(2010 Jul)
Aoki T,Ohnishi H,Oda Y,Tadokoro M,Sasao M,Kato H,Hattori K,Ohgushi H

Gene birth, death, and divergence: the different scenarios of reproduction-related gene evolution.
Biology of reproduction80(4)616-21(2009 Apr)
Tian X,Pascal G,Fouch?court S,Pontarotti P,Monget P

The role of promoter CpG methylation in the epigenetic control of stem cell related genes during differentiation.
Cell cycle (Georgetown, Tex.)8(6)916-24(2009 Mar)
Dansranjavin T,Krehl S,Mueller T,Mueller LP,Schmoll HJ,Dammann RH

Genome-wide profiling of DNA methylation reveals a class of normally methylated CpG island promoters.
PLoS genetics3(10)2023-36(2007 Oct)
Shen L,Kondo Y,Guo Y,Zhang J,Zhang L,Ahmed S,Shu J,Chen X,Waterland RA,Issa JP

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DPPA5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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